American Journal of Medical Genetics 53264-273 (1994)

Encomium: Theodore Puck, a Life in Biophysics Applied to Medicine

Arthur Robinson University of Colorado Health Sciences Center, National Jewish Center for Immunology and Respiratory Medicine, Eleanor Roosevelt Institute, Denver, Colorado

Ted Puck is a unique and outstanding individual. The accompanying autobiography is testament to the amount and variety of significant scientific work he has produced. There is no question that he has made semi- nal contributions to the understanding of human ge- netics, molecular biology, and health. As described in his autobiography, from the time he received his Ph.D. in 1940 he has been dedicated to the application of the quantitative principles of the physical sciences to prob- lems of human biology, particularly those involving medicine. As an example of this desire to have an im- pact on medicine, in 1959 he offered me, a practicing pediatrician, a faculty position in his department. I was the only M.D. in the department, and we have collabo- rated closely and fruitfully ever since.

An unusual thing about Ted is that at his current age of 78 he still continues to work excitedly on major prob- lems of biology which affect medicine. At a time when most molecular biologists are isolating new genes and relating them to specific diseases, important as that is, Ted is trying t o quantitate and define the major prob- lems of mutagenesis, differentiation, and carcinogene- sis, all of which have a tremendous influence on human health.

One of his most creative insights, which is the basis of what we today call somatic cell genetics, is his devel- opment of the “mammalian cell as a micro-organism.’’ He has been aptly described as the “inventor of somatic cell genetics” [Editorial, Nature New Biology, 243:65, May 16, 19731.

He has been constantly devoted to improving the quality of medical and graduate education. For years he ran a course for medical students in biophysics which conveyed to them the excitement and the rigors of the “new biology” of the 1960s. Both locally and na- tionally he has constantly pressed for modernizing medical education with greater emphasis on preventive medicine and the use of quantitative understanding rather than empiricism in diagnosis and treatment of disease.

Received for publication April 20, 1994. Address reprint requests to Arthur Robinson, M.D., National

Jewish Center for Immunology and Respiratory Medicine, 1400 Jackson Street, Denver, CO 80206.

0 1994 Wiley-Liss, Inc.

Remarkable about Ted is his breadth of interests. His continuing interest and involvement in the problems of education in general in this country are evidenced by his membership in the Paideia Associates for Examina- tion of Primary and Secondary Schooling in America and his membership on the Editorial Board of the En- cyclopaedia Britannica. Since 1972 he has been a fellow of the American Academy of Arts and Sciences, an honor not given lightly to a scientist.

I believe Ted has received every significant award for biological science available in this country with the ex- ception of the Nobel prize, and one might well question why he has not received that.

Finally, Ted has always distinguished himself as a warm, generous, and approachable human being. He reads widely, skis intensively, is a marvelous father to his 3 daughters (all M.D.s involved in clinical and re- search medicine), and a wonderful grandfather.

In sum, I have been talking about a remarkable hu- man being with whom I am proud to have had a friend- ship and a professional relationship.

BIBLIOGRAPHY Franck J, French CS, Puck TT (1941): The fluorescence of chlorophyll

and photosynthesis. J Phys Chem 45:1268-1300. Robertson OH, Loosli CG, Puck TT, Bigg E, Miller BF (1941): The pro-

tection of mice against infection with airborne influenza virus by means of propylene glycol vapor. Science 94:612413.

Puck TT (1942): A method for determining the concentration of propy- lene glycol vapor in air. Science 95178.

Robertson OH, Bigg E, Puck TT, Miller BF, Baker Z (1942): Steriliza- tion of air by means of germicidal aerosol mists and vapors. Am J Adv Sci 17:271-280.

Robertson OH, Bigg E, Puck TT, Miller BF (1942): The bactericidal ac- tion of propylene glycol vapor on microorganisms suspended in air I. J Exp Med 75593-610.

Loosli CG, Robertson OH, Puck TT (1943): The production of experi- mental influenza in mice by inhalation of atmospheres containing influenza virus dispersed as fine droplets. J Infect Dis 72:142-153.

Moulton S, Puck TT, Lemon HM (1943): An apparatus for determina- tion of the bacterial content of air. Science 975-52 .

Puck TT, Robertson OH, Lemon HM (1943): The bactericidal action of propylene glycol vapor on microorganisms suspended in air. 11. The influence of various factors on the activity of vapor. J Exp Med 78:387406.

Robertson OH, Puck TT, Lemon HF, Loosli CG (1943): The lethal ef- fect of triethylene glycol vapor on air-borne bacteria and influenza virus. Science 97:142-144.

Wise H, Puck TT, Stral HM (1943): A rapid colorimetric method for the determination of glycols in air. J Biol Chem 150:61-67.

Theodore Puck 265

Hamburger M Jr , Puck TT, Hamburger VG, Johnson MA (1944): Stud- ies on the transmission of hemolytic streptococcus infections. 111. Hemolytic streptococci in the air, floor dust, and bedclothing of hospital wards and their relation to cross infection. J Infect Dis 75:79-94.

Puck TT, Wise H, Robertson OH (1944): A device for automatically controlling the concentration of glycol vapors in the air. J Exp Med 80:377-381.

Robertson OH, Hamburger M, Loosli C, Puck TT, Lemon H, Wise H (1944): A study of the nature and control of air-borne infection in army camps. JAMA 126:993-999.

Robertson OH, Puck TT, Loosli CG, Hamburger M, Lemon H, Wise H (1944): A new approach to the control of air-borne infection. Trans Assoc Am Physicians lviii:171-174.

Hamburger M, Puck TT, Robertson OH (1945): The effect of triethyl- ene glycol and air-borne beta hemolytic streptococci in hospital wards. I. J Infect Dis 76:208-215.

Hamburger M, Hurst V, Robertson OH, Puck TT (1945): The effect of triethvlene dvcol vauor on air-borne beta hemolvtic streotococci in hospital wa&. 111. ?he action of glycol vapors i t low relative hu- midities. J Infect Dis 77:177-189.

Puck TT, Hamburger M, Robertson OH, Hurst V (1945): The effect of triethylene glycol vapor on air-borne hemolytic streptococci in hos- pital wards. 11. The combined action of glycol vapor and dust con- trol measures. J Infect Dis 76:216-225.

Loosii CG, Wise H, Lemon HM, Puck TT, Robertson OH (1946): The oil treatment of bedclothes for the control of dust-borne infection. 11. The use of triton oil emulsion (T-13) as a routine laundry pro- cedure. Am J Hyg 43:105-119.

Puck TT, Robertson OH, Wise H, Loosli CG, Lemon HM (1946): The oil treatment of bedclothes for the control of dust-borne infection. I. Principles underlying the development and use of a satisfactory oil-in-water emulsion. Am J Hyg 43:91-104.

Puck TT, Wise H (1946): Studies in vapor-liquid equilibria. I. A new dynamic method for the determination of vapor pressures of liq- uids. J Phys Chem 50:329-339.

Puck TT, Chaney AL (1946): The dispersal and control of triethylene glycol vapor for aerial disinfection. Indust Hyg Assoc Q 7:lO-16.

Robertson OH, Puck TT, Wise H (1946): The construction and opera- tion of experimental rooms for the study of air-borne infection. J Exp Med 84:559-567.

Loosli CG, Smith MHD, Gauld R, Robertson OH, Puck TT (1947): Con- trol of cross infections in infants’ wards by the use of triethylene glycol vapor. Am J Public Health 37:1385-1398.

Puck TT (1947): The mechanism of aerial disinfection by glycols and other chemical agents. 11. An analysis of the factors governing the efficiency of chemical disinfection of the air. J Exp Med 85:741- 757.

Puck TT (1947): The mechanism of aerial disinfection by glycols and other chemical agents. I. Demonstration that the germicidal action occurs through the agency of the vapor phases. J Exp Med 85: 729-739.

Robertson OH, Loosli CG, Puck TT, Wise H, Lemon HM, Lester W (1947): Tests for the chronic toxicity of propylene glycol and tri- ethylene glycol on monkeys and rats by vapor inhalation and oral administration. J Pharmacol Exp Ther 9552-76.

Wise H, Puck TT (1947): Saturation concentration of triethylene gly- col vapor a t various relative humidities and temperatures. Science 105:556-557.

Dunklin E, Puck TT (1948): The lethal effect of relative humidity on air-borne bacteria. J Exp Med 8587-101.

Puck TT (1948): An automatic dewpoint meter for the determination of condensible vapors. Rev Sci Instruments 19:16-23.

Robertson OH, Appel EM, Puck TT, Lemon HM, Ritter MH (1948): A study of the bactericidal activity in vitro of certain glycols and closely related compounds. J Infect Dis 83:124-137.

Robertson OH, Dunklin EW, Puck TT, Lester W Jr. (1948): The influ- ence of relative humidity on the survival of respiratory pathogens dispersed into the environment. Trans Assoc Am Physicians lvi:280-287.

Lester W Jr , Robertson OH, Puck TT, Wise H (1949): The rate of bac- tericidal action on triethylene glycol vapor on microorganisms dis- persed into the air in small droplets. Am J Hyg 50:175-188.

Puck TT, Marcus PI (1949): Quantitative growth of animal cells in vitro. Postgraduate course presented by the Department of Bio- physics, University of Colorado School of Medicine, Denver, CO (unpublished).

Puck TT (1949): A reversible transformation of T1 bacteriophage. J Bacteriol 57:647-655.

Wise H, Puck TT, Failey CR (1950): Studies in vapor-liquid equilibria. 11. The binary system triethylene glycol-water. J Phys Coll Chem 54:734-741.

Garen A, Puck TT (1951): The first two steps of the invasion of host cells by bacterial viruses. 11. J Exp Med 94:177-189.

Puck TT, Garen A, Cline J (1951): The mechanism ofvirus attachment to host cells. I. The role of ions in the primary reaction. J Exp Med 93:65-88.

Puck TT (1951): Nature of virus-host specificity. Science 117:685 (Abstract).

Nitowsky HM, Puck TT (1952): A modified radioiodine test for thyroid function. J Lab Clin Med 393824-836.

Puck TT (1952): Discussion of Zirkle’s and Tobias’ papers. In: “Sym- posium on Radiobiology.” New York: John Wiley, pp 384-386.

Puck TT, Wasserman K, Fishman AP (1952): Some effects of inorganic ions on the active transport of phenol red by isolated kidney tubules of the flounder. J Cell Comp Physiol40:73-88.

Tolmach LJ, Puck TT (1952): Mechanism of virus attachment to host cells. 111. J Am Chem Sac 774:5551-5552.

Levine S, Puck TT, Sagik BP (1953): An absolute method for assay of virus hemaglutinins. J Exp Med 98:521-531.

Puck TT, Sagik B (1953): Virus and cell interaction with ion exchang- ers. J Exp Med 972307-820.

Puck TT (1953): The first steps of virus invasion. Cold Spring Harb Symp Quant Biol18:149-154.

Puck TT (1953): Biophysics and modern medicine. Colo Q 2:157-169. Puck TT, Tolmach LJ (1954): Mechanism of virus attachment to host

cells. IV. Physicochemical studies of virus and cell surface groups. Arch Biochem Biophys 51:229-245.

Puck TT, Lee HH (1954): Mechanism of cell-wall penetration of viruses. I. An increase in host-cell permeability induced by bacte- riophase infection. J Exp Med 99:481494.

Puck TT, Lee HH (1954): Lysis of cells by viruses and synthetic poly- electrolytes. Microbial Genetics Bulletin 9: 13-16.

Puck TT (1954): Cell attachment and penetration by viruses. In “The Dynamics of Virus and Rickettsia1 Infection”. Edited by Hartman, F.W., Horsfall, F.L. and Kidd, J.G. New York: The Blakiston Co., pp. 16-29.

Sagik B, Puck TT, Levine S (1954): Quantitative aspects of the spon- taneous elution of influenza virus from red cells. J Exp Med 99: 251-260.

Puck TT, Lee HH (1955): Mechanism of cell-wall penetration by viruses. 11. Demonstration of a cyclic permeability change accom- panying virus infection of E . coli B cells. J Exp Med 101:151-175.

Puck TT, Marcus PI (1955): A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: The use of X-irradiated cells to supplying conditioning factors. Proc Natl Acad Sci USA 41:432437.

Fisher HW, Puck TT (19561: On the functions of X-irradiated “feeder” cells in’supporting growth of single mammalian cells. Proc Natl Acad Sci USA 42:900-906.

Marcus PI, Cieciura SJ, Puck TT (1956): Clonal growth in vitro of ep- ithelial cells from normal human tissues. J Exp Med 104:615-630.

Puck TT, Marcus PI, Cieciura SJ (1956): Clonal growth of mammalian cells in vitro-Growth characteristics of colonies from single HeLa cells with and without a “feeder” layer. J Exp Med 103:273-284.

Puck TT, Marcus PI (1956): Action of X-rays on mammalian cells. J Exp Med 103:653-666.

Puck TT, Fisher HW (1956): Genetics of somatic mammalian cells. I. Demonstration of the existence of mutants with different growth requirement in a human cancer cell strain (HeLa). J Exp Med 104:427433.

Puck TT (1956): The growth and genetics of somatic mammalian cells. 23rd Ross Pediatric Research Conference, Winston-Salem, NC, November 8-9 (Abstract).

266 Robinson

Cieciura SJ, Marcus PI, Puck TT (1957): The use of X-irradiated HeLa cell giants to detect latent virus in mammalian cells. Virology 3:426-427.

Puck TT (1957): The effects of radiation on single cells in tissue cul- ture. Radiat Res 7: (Abstract).

Puck TT (1957): Single human cells in vitro. Sci Am 197:91-100. Puck TT (1957): The genetics of somatic mammalian cells. Adv Biol

Med Phys 5:75-101. Puck TT, Cieciura SJ, Fisher HW (1957): Clonal growth in vitro of hu-

man cells with fibroblastic morphology: Comparison growth and genetic characteristics of single epithelioid and fibroblast-like cells from a variety of human organs. J Exp Med 106:145-158.

Puck TT, Morkovin D, Marcus PI, Cieciura SJ (1957): Action ofx-rays on mammalian cells. 11. Survival curves of cells from normal hu- man tissues. J Exp Med 106:485-500.

Puck TT (1957): The mammalian cell as microorganism. Symposium on Growth and Development. In Rudnick D (ed): “Rhythmic and Synthetic Processes in Growth.” Princeton, NJ: Princeton Univer- sity Press, pp 3-17.

Puck TT (1957): The mammalian cell as an independent organism. Part 111. Properties acquired by cells maintained in continuous cul- ture. Ann N Y Acad Sci 5:291-298.

Puck TT (1957): Clones from single mammalian cells. In “4th Interna- tional Poliomyelitis Conference.” Geneva, Switzerland, July 8-12, pp 248-256.

Puck TT (1957): Single cell plating techniques applied to radiobiologic studies in mammalian cells. FASEB J 16: (Abstract).

Sat0 G, Fisher HW, Puck TT (1957): Molecular growth requirements of single mammalian cells. Science 126:961-964.

Puck TT, Cieciura SJ (1957): Studies on the virus carrier state in mammalian cells. In: “Proceedings of the Symposium on Latency and Masking in Viral and Rickettsia1 Infections.” Minneapolis: Burgess Publishing Company, pp 74-79.

Fisher HW, Puck TT, Sat0 G (1958): Molecular growth requirements of single mammalian cells: The action of fetuin in promoting cell attachment to glass. Proc Natl Acad Sci USA 44:PlO.

Lee HH, Puck TT (1958): Single cell techniques in the study of ultra- violet irradiation of mammalian cells. Radiat Res 9: (Abstract).

Marcus PI, Puck TT (1958): The titration of mammalian cell killing by viruses. Bact Proc (Abstract).

Marcus PI, Puck TT (1958): The cell interaction of animal viruses. I. Titration of cell-killing by viruses. Virology 6:405423.

Morkovin D, Puck TT (1958): Single cell techniques in the study of ra- dio-protective action. Radiat Res 9: (Abstract).

Puck TT (1958): Growth and genetics of somatic mammalian cells in vitro. J Cell Comp Physiol 52:287-311.

Puck TT (1958): Action of radiation on mammalian cells. 111. Rela- tionship between reproductive death and induction of chromosome anomalies of X-irradiation of euploid human cells in vitro. Proc Natl Acad Sci USA 44:772-780.

Puck TT (1958): Quantitative measurement of virus action by cell plating technics. In Pollard (ed): “Perspectives in Virology.” pp 88-102.

Puck TT (1958): Quantitative colonial growth of single mammalian cells. Ann N Y Acad Sci 71:1141-1142.

Puck TT, Cieciura SJ, Robinson A (1958): Genetics of somatic mam- malian cells. 111. Long-term cultivation of euploid cells from hu- man and animal subjects. J Exp Med 108:945-956.

Tjio JH, Puck TT (1958): Genetics of somatic mammalian cells. 11. Chromosomal constitution of cells in tissue culture. J Exp Med 108:259-268.

Tjio JH, Puck TT (1958): The somatic chromosomes of man. Proc Natl Acad Sci USA 44:1229-1237.

Fisher HF, Puck TT, Sat0 GA (1959): A “defined” medium for quanti- tative growth of single S3 HeLa cells. Animal Cell Information Ser- vice Newsletter 1: (Abstract).

Fisher HW, Puck TT, Sat0 G (1959): Molecular growth requirements of single mammalian cells. 111. Quantitative colonial growth of sin- gle S3 cells in a medium containing synthetic small molecular con- stituents and two purified protein fractions. J Exp Med 109:649- 660.

Puck TT (1959): Genetic studies on somatic mammalian cells. In “Pro- ceedings of the X International Congress of Genetics 11.” (Abstract).

Puck TT, Robinson A, Tjio J H (1959): The chromosomal constitution of human subjects with genetic diseases 11. Treacher-Collins syn- drome (mandibulo-facial dysostosis). Animal Cell Information Ser- vice Newsletter 1:2 (Abstract).

Puck TT (1959): Lethal effects of radiation on cell reproduction. Radiat Res 11:462.

Puck TT, Robinson A, Tjio J H (1959): The genetic defect in testicular feminization. Animal Cell Information Service Newsletter 1:3 (Abstract).

Puck TT (1959): Radiation effects on mammalian cells in vitro. Animal Cell Information Service Newsletter 1:3 (Abstract).

Puck TT (1959): Quantitative studies on mammalian cells in vitro. Rev Modern Phys 31:433448.

Puck TT (1959): Single mammalian cells. In “Squibb Centennial Lec- tures, 1958-1959.” New York: G.P. Putnam and Sons, pp 1-12.

Puck TT (1959): Cytogenetic studies on human somatic cells in vitro. Science 130:1423-1424.

Puck TT (1959): Memorial to Dr. Raymond R. Lanier. Bulletin, Uni- versity of Colorado School of Medicine 13-15.

Robinson A, Puck TT, Tjio JH (1959): Studies of cells cultivated in vitro from individuals with genetic diseases. “Meeting of Society for Pediatric Research.” Buck Hill Falls, PA, May 6-9 (Abstract).

Tjio JH, Puck TT, Robinson A (1959): Chromosomes ofhuman subjects with genetic defects. Animal Cell Information Service Newsletter 1: (Abstract).

Tjio JH, Puck TT, Robinson A (1959): The somatic chromosomal con- stitution of some human subjects with genetic defects. Proc Natl Acad Sci USA 45:1008-1016.

Fisher HW, Puck TT (1960): Further studies on fetuin: Amino acid and sugar constituents; action of antibodies. Animal Cell Information Service Newsletter 2:2 (Abstract).

Fisher HW, Puck TT (1960): Biophysical studies on fetuin. In “4th An- nual Meeting of Biophysical Society.” Philadelphia, PA, February (Abstract).

Fisher HW, Ham RG, Puck TT (1960): Macromolecular growth requirements of single, diploid, mammalian cells. FASEB J 19: (Abstract).

Gamewell R, Puck TT (1960): A rapid micro method for titration of agents which affect active transport of vital stain by mammalian cells. Animal Cell Information Service Newsletter 2: (Abstract).

Lee HH, Puck TT (1960): The action of ultraviolet radiation on mam- malian cells as studied by single cell techniques. Radiat Res 12: 340-348.

Oda M, Puck TT (1960): Loss of sensitization of mammalian cells treated with specific antibody. Animal Cell Information Service Newsletter 2: (Abstract).

Puck TT, Engelberg J (1960): Application of single cell survival curves for radiation processes in vivo. Radiat Res 12: (Abstract).

Puck TT (1960): Summary of informal discussions. Cancer Res 20:771-772.

Puck TT (1960): Reversible mitotic lag induced by x-irradiation of mammalian cells. Animal Cell Information Service Newsletter 2: (Abstract).

Puck TT (1960): The survival curve of normal human cells with re- spect to x-irradiation. Animal Cell Information Service Newsletter 2: (Abstract).

Puck TT, Robinson A (1960): Additional chromosomal satellites in man. Animal Cell Information Service Newsletter (Abstract).

Puck TT (1960): Quantitative mammalian cell culture: A summary of methods used in the Department of Biophysics of the University of Colorado Medical Center, Denver, CO, pp. 1-60 (unpublished).

Puck TT, Robinson A, Tjio J H (1960): Familial primary amenorrhea due to testicular feminization: Evidence of a human sex-linked gene affecting sex differentiation. Proc SOC Exp Biol Med 103: 192-196.

Puck TT (1960): Radiation and the human cell. Sci Am 202:142-149. Puck TT, Oda M (1960): Antibody specificity of mammalian cells

grown in vitro. FASEB J 19: (Abstract).

Theodore Puck 267

Puck TT (1960): In vitro studies on the radiation biology of mam- malian cells. Prog Biophys Mol Biol 10:237-258.

Puck TT (1960): The action of radiation on mammalian cells. Natural- ist 94:95-109.

Puck TT (1960): Leo Szilard and the science of the twentieth century. Humanist 4:195-200.

Robinson A, Puck TT, Tjio J H (1960): Some applications of human chromosomal analysis to problems in medicine. Am J Dis Child 100:777-778.

Robinson A, Book JA, Chu EHY, Ford CE, Franccaro M, Harnden DG, Hsu TC, Hungerford DA, Lejeune J , Levan A, Makino S, Puck TT, Tjio J H (1960): A proposed standard system of nomenclature of hu- man mitotic chromosomes. JAMA 174:159-162.

Tjio JH, Puck TT, Robinson A (1960): The human chromosomal satel- lites in normal persons and in two patients with Marfan’s syn- drome. Proc Natl Acad Sci USA 46532-539.

Yamada M, Puck TT (1960): Reversible mitotic lag induced by x-irra- diation of mammalian cells. 11. Animal Cell Information Service Newsletter 3: (Abstract).

Fisher HW, OBrien D, Puck TT (1961): The amino acid constitution of fetuin. FASEB J 20:150 (Abstract).

Oda M, Puck TT (1961): The interaction of mammalian cells with an- tibodies. J Exp Med 113599-610.

Puck TT (1961): Quantitation of growth of mammalian cells. In: “The Harvey Lecture.” New York Academic Press, Inc., pp 1-12.

Puck TT (1961): Cellular aspects of irradiation and aging in mammals. FASEB J 20:31-34.

Puck TT (1961): Mammalian cell growth in tissue culture. In Zarrow MX(ed): “Growth in Living Systems.” New York Basic Books, Inc., pp. 181-196.

Puck TT, Yamada M (1961): The mechanism of reversible mitotic in- hibition produced by X-irradiation of mammalian cells in vitro. FASEB J 20:150 (Abstract).

Puck TT (1961): Ionizing radiation and medicine. Modern Med 29:64-74.

Puck TT (1961): Chromosomes and tissue culture. Voice Am Forum Lectures Biol Series 13:l-7.

Robinson A, Puck TT (1961): The sampling of human somatic cells for biochemical and cytogenetic studies. SOC Pediatr Res (Abstract).

Yamada M, Puck TT (1961): Action of radiation on mammalian cells. IV. Reversible mitotic lag in the S3 HeLa cell produced by low doses of X-rays. Proc Natl Acad Sci USA 47:1181-1191.

Fisher HW, OBrien D, Puck TT (1962): The hydrolytic products of fetuin. Arch Biochem Biophys 99:241-248.

Ham RG, Puck TT (1962): A regulated incubator controlling C 0 2 con- centration, humidity and temperature, for use in animal cell cul- ture. Proc SOC Exp Biol Med 8:67-71.

Ham RG, Puck TT (1962): Quantitative colonial growth of isolated mammalian cells. Methods Enzymol 590-119.

Puck TT (1962): Cell culture studies applied to human genetics. In Burdette WJ (ed): “Methodology in Human Genetics.” San Fran- cisco: Holden-Day, Inc., pp. 260-271.

Puck TT, Waldren C, Smith L (1962): Action of fetuin A on the stretch- ing reaction of mammalian cells. Animal Cell Information Service Newsletter 3:9 (Abstract).

Puck TT (1962): Cell accumulation as a result of addition of a specific inhibitor to a random culture. Animal Cell Information Service Newsletter 3: 14.

Puck TT, Yamada M (1962): Chromosomal dynamics in irradiated mammalian cells. Radiat Res 16589 (Abstract).

Puck TT, Yamada M (1962): Comparison of the effects of sub-lethal and supra-lethal x-irradiation on mammalian cells. In “Biophysi- cal Society Meeting.” Washington, D.C., February 14-16 (Ab- stract).

Puck TT (1962): Special responsibilities of the medical school in view of the biological revolution In Comroe J H Jr (ed): “Research and Medical Education.” Evanston, IL: Association of American Med- ical Colleges, pp 217-221.

Puck TT, Steffen J (1963): Life cycle analysis of mammalian cells. I. A method for localizing metabolic events within the life cycle, and its application to the action of colcemide and sub-lethal doses of X- irradiation. Biophys J 3:379-397.

Puck TT, Robinson A, Barnicot NA, Bender MA, Carr DH, Chu EHY, Ferguson-Smith A, Ford CE, Fraccaro M, German J, Hamerton JL, Hirschhorn K, Jacobs PA, Lejeune J, Lenz W, Lindsten J, Miller OJ, Moorhead PS, Patau K, Penrose LS, Schmid W, Stern C, Tjio JH, White MJD (1963): The London Conference on the normal hu- man karyotype. Cytogenetics 2264-268.

Puck TT (1963): Life cycle analysis of mammalian cells in vitro. In “American Society of Cell Biology Meeting.” November (Abstract).

Fisher HW, Puck TT (1964): Protein biosynthesis by mammalian cells cultured in vitro. Biophysical Society Annual Meeting, February 24, 1965. Animal Cell Information Service Newsletter 5:11 (Abstract).

Puck TT (1964): Phasing, mitotic delay, and chromosomal aberrations in mammalian cells. lOlst Annual Meeting of the National Acad- emy of Sciences, Washington, D.C., April 27-29. Science 114565466 (Abstract).

Puck TT (1964): Studies of the life cycle of mammalian cells. Cold Spring Harb Symp Quant Biol29:167-176.

Puck TT, Robinson A (1964): Life cycle analysis in mammalian cells. In “3rd Mammalian Cytology and Somatic Cell Genetics Confer- ences.’’ San Juan, Puerto Rico, November 12-14 (Abstract).

Puck TT (1964): Cellular interpretation of aspects of the acute mam- malian radiation syndrome. Symposium Int SOC Cell Biol3:63-77.

Puck TT (1964): Cellular aspects of the mammalian radiation syn- drome. I. Nucleated cell depletion in the bone marrow. Proc Natl Acad Sci USA 52:152-160.

Puck TT (1964): Life-cycle analysis of mammalian cells. In “Biophysi- cal Society 8th Annual Meeting.” Chicago, IL, February 26-28 (Ab- stract).

Puck TT, Sanders P, Petersen D (1964): Life cycle analysis of mam- malian cells, 11. Cells from the Chinese hamster ovary grown in suspension culture. Biophys J 4:441450.

Puck T, Robinson A (1965): Life cycle analysis of human lymphocytes. 35th Annual Meeting Society for Pediatric Research, Philadelphia, PA, May 4-6, 1965. J Pediatr 67:934 (Abstract).

Puck TT (1965): Cell turnover in mammalian tissues. I. Use of cell de- pletion measurements to estimate X-ray reproductive survival curves. Proc Natl Acad Sci USA 54:1797-1803.

Puck TT (1965): Cell turnover time in mouse tissues. 13th Annual Meeting Radiation Research Society, Philadelphia, PA, May 23-26, 1965. Radiat Res 25:230 (Abstract).

Puck TT (1965): Human implications of the new biology. In: “Univer- sity of Denver, Centennial Symposium,” October 10,1964. Denver: Big Mountain Press, pp 58-74.

Puck TT (1965): Reversible G2 lag of x-irradiated mammalian cells. 1965 Meeting of the National Academy of Sciences. Science 148:667 (Abstract).

Robinson A, Puck TT (1965): Sex chromatin studies in newborns: Pre- sumptive evidence for external factors in human nondisjunction. Science 148:83-85.

Kao FT, Puck TT (1966): Genetic-biochemical studies on mammalian cells with nutritional markers. In “International Congress of Human Genetics.” Chicago, IL (Abstract).

Puck TT, Robinson A, Barr ML, et al. (1966): Chicago Conference: Standardization in human cytogenetics. In Bergsma D (ed): “Birth Defects Original Article Series.” Chicago: National Foundation March of Dimes, pp 1-21.

Puck TT (1966): Cellular aspects of the mammalian radiation syn- drome. Radiat Res 27:272-283.

Puck TT (1966): Life cycle analysis of drug action on mammalian cells. In “Biophysical Society Meeting.” Boston, MA, February (Ab- stract).

Puck TT (1966): Cell depletion and turnover rates in mouse tissues. Radiation Research Society Meeting, Coronado, CA, march, 1966. Radiat Res 27504 (Abstract).

Puck TT, Waldren CA, Tjio J H (1966): Some data bearing on the long term growth of mammalian cells in vitro. In Krohn P (ed): “Topics in the Biology of Aging.” New York: Interscience Publishers, pp 101-123.

Puck TT, Waldren CA (1966): On the relationship between fetuin and the alpha-globulin growth factor of adult mammalian sera. In “6th Annual Meeting of the American Society for Cell Biology.” Hous- ton, TX, November 17-19 (Abstract).

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Puck TT (1966): The mammalian cell. In Cairns J , Stent GS, Watson JD (eds): “Phage and the Origins of Molecular Biology.” Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, pp 275-286.

Puck TT (1966): The scientific revolution in the 20th century. Center for the Study of Democratic Institutions, Santa Barbara, CA, May 4 (Abstract).

Robinson A, Puck TT (1966): The epidemiology of non-disjunction. “American Pediatric Society Annual Meeting, Atlantic City, NJ, April 28. J Pediatr 69:895 (Abstract).

Robinson A, Puck TT (1966): Chromosomal non-disjunction in neo- nates. In “International Congress of Human Genetics.” Chicago, IL (Abstract).

Robinson A, Puck TT (1966): Infective hepatitis and Down’s syndrome. Lancet 1:313 (Abstract).

Tobey RA, Peterson DF, Anderson EC, Puck TT (1966): Life cycle analysis of mammalian cells. 111. The inhibition of division in Chi- nese hamster cells by puromycin and actinomycin. Biophys J

Kao FT, Puck TT (1967): Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the re- quirement for proline. Genetics 55:513-524.

Kao FT, Puck TT (1967): Partial reversion of proline deficiency in a Chinese hamster cell auxotroph. Genetics Society of America, Stanford University, CA, August 31-September 2 (Abstract).

Puck TT, Waldren C, Jones C (1967): The role of fetuin and albumin in mammalian cell growth in vitro. J Cell Biol35:108A (Abstract).

Puck TT, Hill HZ (1967): Enzyme kinetics in mammalian cells. I. Rate constants for metabolism in erythrocytes of normal, galactosemic and heterozygous objects. Proc Natl Acad Sci USA 57:1676-1683.

Puck TT (1967): The biological sciences. In: “The Great Ideas Today.” Chicago: Encyclopaedia Britannica, Inc., pp 196-237.

Puck TT, Kao FT (1967): Genetics of somatic mammalian cells. V. Treatment with 5-bromodeoxyuridine and visible light for isola- tion of nutritionally deficient mutants. Proc Natl Acad Sci USA 58:1227-1234.

Puck TT, Hill HZ (1967): Kinetics of galactose metabolism in mam- malian cells. In “Biophysical Society Annual Meeting.” Houston, TX, February (Abstract).

Puck TT (1967): Improved measurement of Cell depletion and turnover in the bone marrow of irradiated mice. 15th Annual Meeting of the Radiation Research Society, San Juan, Puerto Rico, May 7-11. Radiat Res 31:599 (Abstract).

Puck TT (1967): Preface. In Adler MJ (ed): “The Difference of Man and the Difference it Makes.” New York: Holt, Rinehart and Winston, 1967.

Puck TT, Kao FT (1967): Single-hit survival curves for BUdR-treated Chinese hamster cells illuminated with visible light. Genetics So- ciety of America, Stanford University, CA, August 31-September 2 (Abstract).

Puck TT (1967): Culture tissue. In Lapedes DN (ed): “McGraw-Hill Encyclopedia of Science and Technology.” McGraw-Hill, pp 617- 622.

Robinson A, Puck TT (1967): Studies on chromosomal non-disjunction in man. 11. Am J Hum Genet 19:112-129.

Robinson A, Puck TT (1967): Medicine and the human chromosomes. In “Psychopathology of Mental Development, Part 1. Genetics and Neurophysiiology.” New York: Grunne and Stratton, Inc., pp 1-14.

Robinson A, Goad W, Puck TT, Harris J (1967): Chromosomal non-dis- junction in neonates. In “American Society of Human Genetics Meeting.” December (Abstract).

Tourian A, Puck TT (1967): Studies in phenylketonuria I: In vitro con- version of phenylalanine to tyrosine by tissue homogenates and by single cell suspensions. American Academy of Neurology, San Francisco, CA, April (Abstract).

Hill HZ, Puek TT (1968): Enzyme chain analysis in mammalian cells. Biophys SOC J Abstracts 9A163 (Abstract).

Jones C, Puck TT, Waldren C, Yew F (1968): Mammalian cell growth proteins 11. 8th Annual Meeting American Society Cell Biology, Boston, MA, November 11-14,1968. J Cell Biol39:69A (Abstract).

Kao FT, Puck TT (1968): Measurement of mutagenesis in mammalian cells. “Genetics Society of America Meeting.” Boston, MA, Novem- ber 10-13 (Abstract).

61567-581.

Kao FT, Puck TT (1968): Genetics of somatic mammalian cells. VII. In- duction and isolation of nutritional mutants in Chinese hamster cells. Proc Natl Acad Sci USA 60:1275-1281.

Kao FT, Puck TT (1968): Isolation of nutritionally deficient mutants of Chinese hamster cells. In “XI1 International Congress of Genet- ics.” Tokyo, Japan, August 19-28 (Abstract).

Kao FT, Puck TT (1968): X-ray mutagenesis in mammalian cells. Bio- physical Society Annual Meeting, Pittsburgh, PA, February. Bio- phys SOC J Abstracts 9:A163 (Abstract).

Puck TT, Kao FT (1968): Mutagenesis in mammalian cells in vitro. Autumn Meeting of National Academy of Sciences, Pasadena, CA. Proc Natl Acad Sci USA 61:1153 (Abstract).

Puck TT, Kao FT (1968): Genetics of somatic mammalian cells. VI. Use of an antimetabolite in analysis of gene multiplicity. Proc Natl Acad Sci USA 609%-568.

Puck TT, Waldren CA, Jones C (1968): Mammalian cell growth pro- teins, I. Growth stimulation by fetuin. Proc Natl Acad Sci USA 59: 192-199.

Puck TT, Hill HZ (1968): Galactose enzyme kinetics in normal, galac- tosemic and heterozygous mammalian cells. Genetics Society of America Meeting, Boston, MA, November 10-13. Genetics 60:213 (Abstract).

Puck TT (1968): Biochemical and genetic studies on mammalian cells in vitro. “Oholo” Conference, Tel Aviv, Israel, March 31-April 3. Publ. Symposium on Growth and Differentiation of Cells in vitro. (Abstract).

Puck TT, Robinson A (1968): Some perspectives in human cytogenet- ics. In Cooke RE (ed): “The Biological Basis of Pediatric Practice.” pp. 1407-1422.

Robinson A, Goad W, Puck TT (1968): Chromosomal non-disjunction in neonates. In “American Society of Human Genetics Meeting.” Austin, TX, October 10-13 (Abstract).

Cox DM, Puck TT (1969): Chromosomal non-disjunction: The action of colcemid on Chinese hamster cells in uitro. Cytogenetics 8:158- 169.

Hill HZ, Puck TT (1969): Kinetics of galactose metabolism in mam- malian cells 111. In “Biophysical Society Meeting.” Los Angeles, CA, February 26-March 1 (Abstract).

Kao FT, Johnson RT, Puck TT (1969): Genetics of somatic mammalian cells. VIII. Complementation analysis on virus-fused Chinese hamster cells with nutritional markers. Science 164:312-314.

Kao FT, Chasin L, Puck TT (1969): Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Proc Natl Acad Sci USA 64:1284-1291.

Kao FT, Puck TT, Chasin L (1969): Single gene mutations in mam- malian somatic cell. In “National Academy of Sciences Autumn Meeting.” Hanover, NH, October 13-15 (Abstract).

Kao FT, Chasin L, Puck TT (1969): Identification of complementation classes and biochemical analysis in thirteen glycine-requiring Chinese hamster cell mutants. In “9th Annual Meeting of the American Society for Cell Biology.” Detroit, MI, November 6-8 (Abstract).

Kao FT, Puck TT (1969): Genetics of somatic mammalian cells, I X Quantitation of mutagenesis by physical and chemical agents. J Cell Physiol 74:245-258.

Puck TT (1969): Possible applications of mammalian cell methodolo- gies to problems in psychobiology. In “Winter Conference on Brain Research.” Snowmass at Aspen, CO, January 12-16 (Abstract).

Robinson A, Goad WB, Puck TT, Harris J S (1969): Studies on chro- mosomal non-disjunction in man. 111. Am J Hum Genet 21:466- 485.

Robinson A, Puck TT (1969): Chromosomal non-disjunction in neonates. In “3rd International Congress of Cogenital Malforma- tions.” Netherlands, September 8-12 (Abstract).

Tourian A, Goddard J , Puck TT (1969): Phenylalanine hydroxylase ac- tivity in mammalian cells. J Cell Physiol 73:159-170.

Fulwyler ML, Puck TT, Wuthier P (1970): Electric cell separation: Vol- ume distribution of colony-forming stem cells in mouse bone mar- row. In “Biophysical Society Meeting.” Baltimore, MD, February 2 5-2 7 (Abstract).

Hill HZ, Puck TT (1970): Enzyme kinetics in mammalian cells. 11. Si- multaneous determination of rate constants for the first three steps of galactose metabolism in red cells. J Cell Physiol 75:49-56.

Theodore Puck 269

Kao FT, Puck TT (1970): Genetics of somatic mammalian cells: Link- age studies with human-Chinese hamster cell hybrids. Nature 228:329-332.

Kao FT, Puck TT, Jones C (1970): In vitro linkage studies using hu- man-Chinese hamster cell hybrids. In “American Society for Cell Biology Meeting.” San Diego, CA, November 19-21 (Abstract).

Kao FT, Puck TT (1970): A quantitative system for measurement of mutagenesis in mammalian cells. In “1st Annual Meeting of the Environmental Mutagen Society.” Washington, D.C., March 22-25 (Abstract).

Puck TT (1970): Some considerations on genetic analysis at the single gene level in man. Ann N Y Acad Sci 171:406-421.

Puck TT (1970): Mammalian cell genetic studies in vitro. In “Confer- ence on Evaluation Mutagenicity of Drugs and Other Chemical Agents.” Washington, D.C., November 4-6 (Abstract).

Puck TT (1970); Biochemical genetic studies on mammalian cells in vitro. In: “Control Mechanisms in Expression of Cellular Pheno- types.” New York:Academic Press, pp 135-146.

Yew FF, Jones C, Waldren CA, Puck TT (1970): Fetal specific proteins from fetal bovine serum. In “Biophysical Society 14th Annual Meeting.” Baltimore, MD, February 25-27 (Abstract).

Hsie AW, Puck TT (1971): Morphological transformation of Chinese hamster cells by dibutyryl adnosine cyclic 3’:5’-monophosphate and testosterone. Proc Natl Acad Sci USA 68:358-361.

Hsie AW, Jones C, Puck TT (1971): Further changes in differentiation state accompanying the conversion of Chinese hamster cells to fi- broblast form by dibutyryl adenosine cyclic 3’5’-monophosphate and testosterone. Proc Natl Acad Sci USA 68:1648-1652.

Hsie AW, Jones C, Puck TT (1971): Changes in differentiation state accompanying the conversion of Chinese hamster cells to fibrob- lastic form by dibutyryl cyclic 3’:5”-monophosphate and hormones, cyclic AMP. In “Meeting of the American Society for Cell Biology.” New Orleans, LA, November 18-20 (Abstract).

Kao FT, Puck TT (1971): Linkage analysis in human somatic cell by hybridization with selected Chinese hamster mutants. In “4th In- ternational Congress of Human Genetics.” Paris, France, Septem- ber 6-11 (Abstract).

Kao FT, Puck TT (1971): Genetics of somatic mammalian cells. XII. Mutagenesis by carcinogenic nitroso compounds. J Cell Physiol 78:139-144.

Kao FT, Puck TT (1971): Identification of eight genes in Chinese ham- ster auxotrophic mutants by cell hybridization and complementa- tion analysis. In “Meeting of the American Society for Cell Biol- ogy.” New Orleans, LA, November 18-20 (Abstract).

Puck TT (1971): Biochemical and genetic studies on mammalian cells. In Vitro 7:115-119.

Puck TT (1971): Mutation in mammalian cell cultures. In “4th Inter- national Congress of Human Genetics.” Paris, France, September 6-11 (Abstract).

Puck TT, Wuthier P, Jones C, Kao FT (1971): Genetics of somatic mammalian cells: Lethal antigens as genetic markers for study of human linkage groups. Proc Natl Acad Sci USA 68:3102-3106.

Hill HZ, Puck TT (1972): Incorporation of ‘‘C-galactose into tissue cul- ture cells of various origins. In “Mammalian Cytogenetics and So- matic Cell Genetics Conference.” Snowmass, CO, January 18-20 (Abstract).

Hsie AW, Puck TT (1972): Production of variants with respect to re- verse transformation in cultured Chinese hamster cells. American Society for Cell Biology Meeting.” St. Louis, MO, November 8-11. J Cell Biol 55:118A (Abstract).

Jones C , Wuthier P, Kao FT, Puck TT (1972): Genetics of somatic mammalian cells. XV. Evidence for linkage between human genes for lactic dehydrogenase B and serine hydroxymethylase. J Cell Physiol 80:291-298.

Kao FT, Puck TT (1972): Genetics of somatic mammalian cells. XIV. Genetic analysis in vitro of auxotrophic mutants. J Cell Physiol

Kao FT, Puck TT (1972): Genetics of somatic mammalian cells: Demonstration of a human esterase activator gene linked to the AdeB gene (XVI in series). Proc Natl Acad Sci USA 69:3273-3277.

Kao FT, Puck TT (1972): Linkage between a human adenine gene and a gene regulating esterase activity. In “American Society for Cell Biology Meeting.” St. Louis, MO, November 8-11 (Abstract).

80:41-50.

Puck TT (1972): “The Mammalian Cell as a Micro-Organism: Genetics and Biochemical Studies in vitro”. San Francisco:Holden-Day, Inc., pp 1-219.

Puck TT, Waldren CA, Hsie AW (1972): Membrane dynamics in the action of dibutyryl adenosine cyclic 3’5’-monophosphate and testosterone on mammalian cells. Proc Natl Acad Sci USA 69: 1943-1947.

Puck TT (1972): Foreword. In Thomas C (ed): “Perspectives in Cyto- genetics.”

Puck TT (1972): What can the new biology offer man? In: “Biology in Human Affairs.” pp 111-123.

Wuthier PC, Jones C, Puck TT (1972): Immunologic analysis of lethal antigens in somatic mammalian cells and cell-hybrids. In “Ameri- can Society for Cell Biology Meeting.” St. Louis, MO, November 8-1 1 (Abstract).

Hill HZ, Puck TT (1973): Detection of inborn errors of metabolism: Galactosemia. Science 179:1136-1139.

Jones C, Puck TT (1973): Genetics of somatic mammalian cells. XVII. Induction and isolation of Chinese hamster cell mutants requiring serine. J Cell Physiol 81:299-304.

Kao FT, Puck TT (1973): Quantitation of mutagenesis by the BudR- visible light technique. (Abstract).

Kauvar AJ, Wenger L, Puck TT (1973): The measurement of cell ad- hesivity in vitro. ICRS, November (Abstract).

Puck TT (1973): Somatic cell genetic methods applicable to neurobio- logical research. In “Molecular Neurobiology Symposium, the Given Institute of Pathobiology.” Aspen, CO, June 18-20 (Abstract).

Puck TT, Wenger L (1973): Substitution of testololactone for testos- terone in the “reverse transformation” of Chinese hamster cells. International Research Communications System.

Puck TT, Jones C (1973): Cyclic AMP and microtubular dynamics in cultured mammalian cells. In Braun W, Lichtenstein LM, Parker CW (eds): “Cyclic AMP, Cell Growth and the Immune Response.”

Puck TT, Jones C, Wuthier P (1973): Genetics of mammalian cell sur- face antigens. In “American Society for Cell Biology Meeting.” Mi- ami Beach, FL, November 14-17 (Abstract).

Puck TT (1973): Genetic-biochemical studies in mammalian cells. In “Fifth Annual Winter Symposia, University of Miami and the Pa- panicolaou Cancer Research Institute.” Miami, FL, January 14-19 (Abstract).

Puck TT (1973): Genetic biochemical studies on the mammalian cell surface. In: “The Role of Cyclic Nucleotides in Carcinogenesis.” New York: Academic Press, pp 283-302.

Puck TT, Kao FT (1973): Sufficient criteria for mutagenesis in somatic mammalian cells. Mutat Res 21:230-231 (Abstract).

Puck TT (1973): Genetic biochemical studies on cell surface phenom- ena (Abstract).

Robinson A, Goad W, Puck TT (1973): Epidemiology of chromosomal aneuploidy. In “American Pediatrics Society Meeting.” San Fran- cisco, CA, May 19 (Abstract).

Wuthier P, Jones C, Puck TT (1973): Surface antigens of mammalian cells as genetic markers. 11. J Exp Med 183:229-244.

Jones C, Wuthier P, Puck TT (1974): Application of somatic cell ge- netics to surface antigens. In Moscona AA (ed): “The Cell Surface in Development.” New York: John Wiley and Sons, pp 283-296.

Kao FT, Puck TT (1974): Induction and isolation of auxotrophic mutants in mammalian cells. In Prescott D (ed): “Methods in Cell Biology, Vol. VIII.” New York: Academic Press, Inc., pp 23-39.

Patterson D, Kao FT, Puck TT (1974): Genetics of somatic mammalian cells: Biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism. Proc Natl Acad Sci USA 71:2057- 2061.

Porter KR, Puck TT, Hsie AW, Kelley D (1974): An electron micro- scope study of the effects of dibutyryl cyclic AMP on Chinese ham- ster ovary cells. Cell 2:145-162.

Puck TT (1974): The action of cyclic AMP on mammalian cell in vitro. In “Biochemistry and Biophysics Meeting.” Minneapolis, MN, June 2-7 (Abstract).

Puck TT (1974): Hybridization and somatic cell genetics. In Davidson RL, de la Cruz F (eds): “Somatic Cell Hybridization.” New York Raven Press, pp 27-34.

pp. 338-348.

270 Robinson

Puck TT (1974): Genetics of somatic mammalian cells. In: “Genetics Today.” New YorkCRM Books, Random House, Inc., pp 47-60.

Puck TT (1974): Absence of a demonstrable cGMP effect on the CAMP- induced reverse transformation of Chinese hamster ovary (CHO) cells. J Cell Biol63:275A (Abstract).

Jones C, Wuthier P, Puck TT (1975): Genetics of somatic cell surface antigens. 111. Further analysis of the AL marker. Somat Cell Genet

Kao FT, Jones C, Puck TT (1975): Human-Chinese hamster cell hy- brids containing single human chromosomes. American Society for Cell Biology, San Juan, Puerto Rico, November 11-14 (Abstract).

Kao FT, Puck TT (1975): Mutagenesis and genetic analysis with Chi- nese hamster auxotrophic cell markers. Genetics 79:343-352.

Moore B, Jones C, Puck TT (1975): Immunological correspondence be- tween the a, human cell surface antigen and glycophorin. Ameri- can Society for Cell Biology, San Juan, Puerto Rico, November 11-14, 1975. J Cell Biol67:292A (Abstract).

Puck TT, Jones C, Kao FT, Patterson D, Waldren CA (1975): Use of Chinese hamster cells for human genetic analysis. American Soci- ety of Human Genetics, Baltimore, MD, October 8-11 (Abstract).

Puck TT (1975): Somatic cell genetics and its human applications. In Meints RH, Davies E (eds): “Control Mechanisms in Develop- ment.” New York: Plenum Press.

Dahl RH, Morrissey A, Puck TT, Morse ML (1976): Carboyhydrate en- ergy sources for Chinese hamster cells in culture (39521). Proc SOC Exp Biol Med 153:251-253.

Goad WB, Robinson A, Puck TT (1976): Incidence of aneuploidy in a human population. Am J Hum Genet 28:62-68.

Jones C, Kao FT, Moore B, Patterson D, Puck TT (1976): Human gene locations achieved by means of human-Chinese hamster (CHO-K1) auxotrophic cell hybridization. Cytogenet Cell Genet 16:387-390.

Kao FT, Jones C, Puck TT (1976): Genetics of somatic mammalian cells: Genetic, immunologic and biochemical analysis with Chinese hamster hybrids containing selected human chromosomes. Proc Natl Acad Sci USA 73:193-197.

Moore E, Jones C, Puck TT (1976): Cell surface antigens. IV. Immunological correspondence between glycophorin and the a1 human cell surface antigen. Cytogenet Cell Genet 17:89-97.

Puck TT (1976): Mammalian cell regulation. In Fenoglio CM, King DW (eds): “Advances in Pathobiology IV. Cancer Biology, Etiology and Therapy.” New York: Stratton Intercontinental Book Corp., pp 72-80.

Puck TT (1976): Somatic cell biology and the medical curriculum. In Purcell E (ed): “Recent Trends in Medical Education in the U.S.” New York: Josiah Macy, Jr. Foundation, pp 144-150.

Puck TT (1976): Some suggestions on the role of schools of public health in the coming period. (UnPublished).

Jones C, Puck TT (1977): Further studies on hybrid cell-surface anti- gens associated with human chromosome 11. Somat Cell Genet 3:407420.

Kao FT, Jones C, Puck TT (1977): Regional mapping on human chro- mosome 11 of five loci: Three human cell surface antigen markers, LDH-A and ACP-2. In “Annual Meeting of the American Society of Human Genetics.” San Diego, CA, October 19-22 (Abstract).

Kao FT, Jones C, Puck TT (1977): Genetics of cell surface antigens. VII. Regional mapping of three components on the human cell sur- face antigen complex, AL, on chromosome 11. Somat Cell Genet 3:421429.

Meek WD, Porter KR, Puck TT (1977): Scanning electron microscopy (SEM) studies on the effect of cyclic AMP upon dendrite-like ex- tension in a Chinese hamster ovary-Chinese hamster brain hybrid. “American Society of Cell Biology Annual Meeting.” San Diego, CA, November 15-19 (Abstract).

Puck TT (1977): Somatic cell genetics in problems related to differen- tiation. In Borek C, Fenoglio CM, King DW (eds): “Cancer Biology IV. Differentiation and Carcinogenesis.” New York: Stratton Books, pp 4-17.

Puck TT (1977): Cyclic AMP, the microtubule-microfilament system, and cancer. Proc Natl Acad Sci USA 74:44914495.

Puck TT (1977): Studies in mammalian cell regulation: Cell surface antigens and the action of cyclic AMP. In Ts’o POP (ed): “The Mol- ecular Biology of the Mammalian Genetic Apparatus, Vol. 11, Part B.” Amsterdam, Ny: North Holland Publishing C., pp 171-180.

1:235-246.

Puck TT (1977): Cyclic AMP, microtubules, microfibrils, and cancer. In Wilcox G, Abelson J, Fox CF (eds): “Eukaryotic Genetics System: ICN UCLA Symposium on Molecular and Cellular Biology VIII.” New York: Academic Press, pp 399-111.

Puck TT (1977): The Gordon Wilson Lecture: The new cell biology and its implications for medicine. Trans Am Clin Climat Assoc 89: 77-84.

Rao PM, Wilson B, Puck TT (1977): Premature chromosome conden- sation and cell cycle analysis. J Cell Physiol 91:131-141.

Waldren C, Jones C, Puck TT (1977): Production of single gene muta- tion, deletion and chromosome loss in the human-Chinese hamster ovary cell hybrid containing human chromosome 11. In “American Society of Cell Biology Annual Meeting.” San Diego, CA, Novem- ber, 1977. J Cell Biol75:393A (Abstract).

Kao FT, Jones C, Law ML, Puck TT (1978): Regional assignment of genes on human chromosomes 11 and 12. Cytogenet Cell Genet 22:474-477.

Kao FT, Faik P, Puck TT (1978): Isolation and characterization of cell hybrids formed between normal human brain cells and Chinese hamster auxotrophic mutants. In “14th International Congress of Genetics.” Moscow, USSR, August 21-30 (Abstract).

Meek WD, Porter KR, Puck TT (1978): Neurite formation following treatment with dibutyryl cyclic AMP of a Chinese hamster ovary (CHO) X Chinese hamster brain cell hybrid. 11. A transmission electron microscopy (TEM) study. J Cell Biol 79:295a (Abstract).

Puck TT (1978): The genetics of somatic mammalian cells. In Cohen BH, Lilienfeld AM, Huang DC (eds): “Genetic Issues in Public Health and Medicine.” Charles C. Thomas, pp 50-59.

Puck TT, Kao FT (1978): Mammalian cell genetic studies in relation- ship to cancer. In “10th International Cancer Congress.” Houston, TX, May 22-29 (Abstract).

Robinson A, Goad WB, Puck TT (1978): Epidemiology of chromosomal aneuploidy. In Cohen BH, Lilienfeld AM, Huang DC (eds): “Ge- netic Issues in Public Health.” Charles C. Thomas, pp 206-214.

Storrie B, Puck TT, Wenger L (1978): The role of butyrate in the re- verse transformation reaction in mammalian cells. J Cell Physiol 94:69-75.

Gusella J , Housman D, Kao FT, Jones C, Puck TT, Varsanyi-Breiner A, Keys C, Orkin S (1979): Precise localization of the human B-glo- bin gene complex on chromosome 11. Proc Natl Acad Sci USA

Housman D, Gusella J , Kao FT, Jones C, Breiner A, Keys C, Orkin S, Puck TT (1979): Regional mapping of human structural gene for hemoglobin p on chromosome 11 using restriction endonuclease mapping and a regional clone panel. (Abstract).

Jones C, Puck TT (1979): Immunogenetics of mammalian cell surface macromolecules. In Monroe A, Friedlander M, Moscoa A A (eds): “Current Topics in Developmental Biology.” pp. 89-115.

Kao FT, Faik P, Puck TT (1979): Extension of branching processes from hybrids of brain and Chinese hamster ovary cells. Exp Cell Res 122:83-91.

McConkey EH, Kao FT, Puck TT (1979): Gene products of specific chromosomes: A new approach. Am J Hum Genet 31:54A (Abstract).

Meek W, Puck TT (1979): Role of microfibrillar system in knob action of transformed cells. J Supramol Structure 12:335-354.

Meek WD, Puck TT (1979): Role of the microfibrillar system in knob action of transformed cells. In: “ICN-UCLA Symposia, Molecular Biology Institute.” Los Angeles: University of California (Abstract).

Puck TT (1979): Studies on cell transformation. Somat Cell Genet 5:973-990.

Puck TT (1979): Cyclic AMP, the microfibrillar system, and cell regu- lation. (Abstract).

Puck TT (1979): Historical perspective on mutation studies with so- matic mammalian cells. In “Banbury Report 11: Mammalian Cell Mutagenesis, The Maturation of Test Systems.” Cold Spring Har- bor, Ny: Cold Spring Harbor Laboratory, pp 3-12.

Waldren C, Jones C, Puck TT (1979): Measurement of mutagenesis in mammalian cells. Proc Natl Acad Sci USA 76:1358-1362.

Gusella JF, Keys C, Varsanyi-Breiner A, Kao FT, Jones C, Puck TT, Housman D (1980): Isolation and localization of DNA segments from specific human chromosomes. Proc Natl Acad Sci USA 77:2829-2833.

76:5239-5243.

Theodore Puck 271

Jones C, Puck TT, Kao FT (1980): Immunologic studies related to ma- lignancy. ICRDB special listing on tumor-related antigens: Their identification, isolation, and characterization, February 25 (Abstract).

Kao FT, Jones C, Puck TT, Law ML, Gusella J, Keys C, Varsanyi- Breiner A, Housman D (1980): Human DNA segments as genetic markers (Abstract).

Meek WD, Porter KR, Puck TT (1980): The ultrastructure of process formation following treatment with dibutyryl cyclic AMP of a Chi- nese hamster ovary x Chinese hamster brain cell hybrid. Exp Cell Res 126:359-374.

Nielson SE. Puck TT (1980): Surface deDosition of fibronectin in the course of reverse transformation of C?HO cells of cyclic AMP. Proc Natl Acad Sci USA 77:985-989.

Puck TT, Meek WD, Nielson SE, Erikson RL (1980): Morphological ev- idence for cyclic AMP-induced reverse transformation in vole cells infected with avian sarcoma virus (ASV). ASCB Meeting, Cincin- nati, OH, November 14-18, 1980. J Cell Biol87:314A (Abstract).

Puck TT (1980): The role of the cell surface and microfibrils in trans- formation. In Borek C, Fenglio C, Kmg DW (eds): “Advances in Pathobiology.” New York: Theime-Stratton, Inc., pp 331-349.

Puck TT (1980): Cyclic AMP, microfibrils, and cancer. (Abstract). Scoggin CH, Davidson JN, Patterson D, Puck TT (1980): Two-dimen-

sional electrophoresis of proteins representing gene expression by chromosomes 21 and 11. Clin Res 28:290A (Abstract).

Gabrielson EW, Puck TT, Scoggin CH (1981): Reverse transformation of malignant cells is associated with increased phosphorylation of cellular proteins. Western Society for Clinical Research, February p6 (Abstract).

Puck TT (1981): The mapping of the human genes and its implications in developmental disease. In Hill RB (ed): “Environmental Pathol- ogy: Elements of a Curriculum of Students in Medicine.” pp 97-110.

Puck TT, Erikson RL, Meek WD, Nielson SE (1981): Reverse trans- formation of vole cells transformed by avian sarcoma virus con- taining the src gene. AACR Meeting, April 30-May 2 (Abstract).

Puck TT, Erikson RL, Meek WD, Nielson SE (1981): Reverse trans- formation of vole cells transformed by avian sarcoma virus con- taining the src gene. J Cell Physiol 107:399412.

Puck TT (1981): Cyclic AMP and the src gene in cancer. (Abstract). Puck TT (1981): Some new developments in genetic analysis of so-

matic mammalian cells. In: “Control of Cellular Division and Development: Part B.” pp. 393403.

Puck TT (1981): Somatic cell genetics and genetic biochemistry. (Un- Published)

Scoggin CH, Gabrielson E, Davidson JN, Jones C, Patterson D, Puck TT (1981): Two-dimensional electrophoresis of human-CHO cell hybrids containing human chromosome 11. Somat Cell Genet 7:389-398.

Scoggin CH, Gabrielson E, Davidson JN, Jones C, Patterson D, Kao FT, Puck TT (1981): Gene expression by two-dimensional gel analysis of peptides from mapped loci on human chromosomes. The 32nd Annual Meeting of the American Society of Human Genetics, Dallas, TX, October 28-31. Am J Hum Genet 33:149A (Abstract).

Gabrielson EG, Scoggin C, Puck TT (1982): Phosphorylation changes induced by cyclic AMP derivatives in the CHO cell and selected mutants. Exp Cell Res 142:63-68.

Gusella JF, Jones C, Kao FT, Housman D, Puck TT (1982): Genetic fine structure mapping in human chromosome 11 by use of repeti- tive DNA sequences. Proc Natl Acad Sci USA 79:7804-7808.

Puck TT (1982): Somatic cell genetics and its application to medicine (Abstract).

Puck TT (1982): Tissue culture. In: “McGraw-Hill Encyclopedia of Sci- ence and Technology”. New York: McGraw-Hill Book Company,

Puck TT, Kao FT (1982): Somatic cell genetics and its application to medicine. Annu Rev Genet 16:255-271.

Puck TT (1982): Environmental pathology. Radiation biology in mam- malian cell systems. In: “An Evolving Field.” New York: Alan R. Liss, Inc., pp 77-95.

Rumsby G, Puck TT (1982): Ornithine decarboxylase induction and the cytoskeleton in normal and transformed cells. J Cell Physiol

pp 739-744.

1111133-139.

Waldren CA, Puck TT, Cram S (1982): The use of fluorescence acti- vated cell sorting (FACS) to reduce the background and increase the resolving power of the AL mutagen assay. Environ Mutagen 5103 (Abstract).

Nielson SE, Rumsby G, Davidson J, Mockus M, Kao FT, Jones C, Cleveland D, Aiba I, Puck TT (1983): Mapping studies on human genes. 7th International Workshop on Human Gene Mapping, Los Angeles, CA, August 21-26. (Abstract).

Puck TT (1983): Human gene mapping, exposure and expression. In Caskey CT, White RL (eds): “Banbury Report 14.” Cold Spring Harbor, Ny: Cold Spring Harbor Laboratory, pp 205-213.

Puck TT (1983): Tissue culture. In: “Encyclopaedia Britannica.” Chicago: Encyclopaedia Britannica, pp 438442.

Puck TT, Marcus PI, Cieciura SJ (1983): Clonal growth of mammalian cells in vitro: Growth characteristics of colonies from single HeLa cells with and without a “feeder” layer. Current Contents, Citation Classics January 17: (Abstract).

Puck TT (1983): Somatic cell genetics and its human implications. In “Annual Meeting of the American Association for the Advance- ment of Science.” Detroit, MI, May 28. (Abstract).

Puck TT (1983): The coming era in medicine. In “Frontiers of Medicine Conference.” Denver, CO, November 19. (Abstract).

Puck TT (1983): Somatic cell genetics and the radiation biology of mammalian cells. Annual Meeting Radiation Research Society, Orlando, FL, March 26 (Abstract).

Puck TT (1983): Human gene mapping by deletion analysis. LANIJGenes, Chromosomes and Flow Cytometry, Santa Fe, NM, October 23-26. (Abstract).

Schonberg S, Patterson D, Puck TT (1983): Resistance of Chinese hamster ovary cell chromatin to endonuclease digestion I. Rever- sal by CAMP. Exp Cell Res 145:57-62.

Waldren CA, Puck TT, Cram S (1983): Improved mutagenesis mea- surement by FACS. LANWGenes, Chromosomes and Flow Cytom- etry, Santa Fe, NM, October 23-26 (Abstract).

Ashall F, Puck TT (1984): Cytoskeletal involvement in CAMP-induced sensitization of chromatin to nuclease digestion in transformed Chinese hamster ovary K1 cells. Proc Natl Acad Sci USA 81:5 145-5149.

Cheung P, Kao FT, Law ML, Jones C, Puck TT, Chan L (1984): Local- ization of the structural gene for human apolipoprotein A-I on the long arm of human chromosome 11. Proc Natl Acad Sci USA 81:508-51 1.

Cowan D, Puck TT (1984): The teaching of science. (Unpublished) Cowan D, Puck TT (1984): What is science? In: “The Paideia Program:

An Education Syllabus.” New York: MacMillan Publishing Com- pany, pp 8 6 1 0 8 .

Law ML, Kao FT, Jones C, Puck TT (1984): Use of cloned repetitive DNA sequences for mapping human chromosomes. 7th Interna- tional Workshop for Human Gene Mapping, Los Angeles, CA, Au- gust 21-26, 1983. Cytogenet Cell Genet 37519-520 (Abstract).

Puck TT (1984): Transformation and reverse transformation in mam- malian cells. Adv Viral Oncol4:197-216.

Puck TT (1984): The biology of cell transformation to malignancy. Physician’s Journal Update-Segment on Oncogenes. Aired on LIFETIME, April 25,28,29. (Unpublished)

Puck TT (1984): The teaching of science in a democracy. (UnPub- lished)

Puck TT (1985): Quantitation of mutagenesis in mammalian cells. (Abstract).

Puck TT (1985): Development of the Chinese hamster ovary (CHO) cell for use in somatic cell genetics. In Gottesman M (ed): “Molecu- lar Cell Genetics.” pp 37-64.

Puck TT (1985): The new biology and its human implications. In: “The Great Ideas Today.” Chicago: Encyclopaedia Britannica, Inc., pp 52-78.

Puck TT (1985): Consideration of measurements and calculations in- volved in assessment of the effects of human exposure to low doses of ionizing radiation and other mutagens. Let Math Phys 10: 225-230.

Meek W, Nielson S, Puck TT (1986): Studies on cytoskeletal structure and function. (Abstract).

Puck TT, Waldren CW, Jones C (1986): Quantitative measurement of mutation in mammalian cells. American Society of Human Genet- ics Meeting, Philadelphia, PA, November 2-5. (Abstract).

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Puck TT (1986): Declaration of Independence. (UnPublished). Puck TT (1986): Cellular and molecular studies in human chromoso-

mal diseases. In Ratcliffe ST, Paul N (eds): “Prospective Studies on Children with Sex Chromosome Aneuploidy.” New York: Alan R. Liss, Inc., pp 307-312.

Waldren C, Correll L, Sognier M, Puck TT (1986): The measurement of low levels of X-ray mutagenesis in relation to human disease. Proc Natl Acad Sci USA 83:48394843.

Anderson HL, Puck TT, Shera EB (1987): A new apparatus for direct counting of B-rays from 2-D protein gels: Measurement of changes in protein synthesis due to changes in density of CHO cells. Proc Natl Acad Sci USA 84:47494753.

Law ML, Kao FT, Wei Q, Hartz JA, Green GL, Zarucki-Schulz T, Con- neely OM, Puck TT, O’Malley BW, Horwitz KB (1987): The prog- esterone receptor gene maps to human chromosome band llq13, site of the mammary oncogene int-2. Proc Natl Acad Sci USA 84:2877-2881.

Puck TT (1987): Reverse transformation as a model for cytoskeletal control of gene exposure. Differentiation of Normal and Neoplastic Cells Meeting, Boulder, CO, August 3-7. (Abstract).

Puck TT (1987): Genetic regulation of growth control: Role of cyclic AMP and cell cytoskeleton. Somat Cell Mol Genet 13:451457.

Puck TT, Waldren CA (1987): Mutation in mammalian cells: Theory and implications. Somat Cell Mol Genet 13:405409.

Puck TT, Davidson RL (1987): Conference proceedings: The next 25 years in human genetics. Somat Cell Mol Genet 13:359-360.

Puck TT (1987): The new biology and its human implications. In “Emerging Syntheses in Science.” Addison-Wesley Publishing Company, 1987.

Puck TT (1987): The nature, treatment and prevention of cancer. The 1987 Aspen Cancer Conference, Aspen, CO, July 20-22. Toxicol Pathol 16:64-66 (Abstract).

Waldren C, Puck TT (1987): Steps toward experimental measurement of total mutations relevant to human disease. Somat Cell Mol Genet 13:411414.

Waldren C, Jones C, Puck TT (1987): A new approach to measurement of mutagenesis in mammalian cells. Annual Aspen Lung Confer- ence, Aspen, CO, June 11-14. Chest 91S:45S (Abstract).

Waldren CA, Bedford JS, Hei T, Puck TT (1987): Analysis of mutage- nesis in mammalian cells. In “8th International Congress of Radi- ation Research.” Edinburgh, Scotland, July 19-24. (Abstract).

Ashall F, Sullivan N, Puck TT (1988): Specificity of the CAMP-induced gene exposure reaction in CHO cells. Proc Natl Acad Sci USA 85:3908-3912.

Chan DCF, Puck TT (1988): In vivo thiophosphorylation is not equiv- alent to phosphorylation: A high resolution two-dimensional gel electrophoresis study. 32nd Annual Meeting of the Biophysical Society, Phoenix, AZ, February 28-March 3. Biophys J 53:300a (Abstract).

Parker R, Waldren C, Hei TK, Wong DF, Puck TT (1988): Analysis of mutant frequency curves and survival curves applied to the AL hy- brid cell system. J Theor Biol 132:113-117.

Puck TT, Waldren C (1988): Measurement of mutation in mammalian cells. Environmental Mutagen Society 19th Annual Scientific Meeting, Charleston, SC, March 27-31. Environ Mol Mutagen 11234 (Abstract).

Puck TT (1988): Measurement of mutagenesis in mammalian cells. In “Meeting of Somatic Cell Genetics Society.” Santa Fe, NM, Janu- ary 13 (Abstract).

Puck TT (1988): Mutational disease in man. Los Alamos Colloquium, Los Alamos, NM, March 3. (Abstract).

Waldren CA, Puck TT (1988): Measurement of mutation yields. In “The Fifth Health Effects Institute Annual Conference.” Colorado Springs, CO, April 17-20 (Abstract).

Chan D, Goate A, Puck TT (1989): Involvement of vimentin in the re- verse transformation reaction. Proc Natl Acad Sci USA 86:2747- 2751.

Curlander JB, Meech SJ, Odom LF, McGavran L, Puck TT (1989): Dif- ferences in colchicine induced aneuploidy in transformed Chinese hamster ovary (CHO) cell lines and primary cultures of human kidney epithelian cells. Annual Meeting Western Student Medical Research Committee, Carmel, CA, February 7-10. Clin Res 37:221A (Abstract).

Krystosek A, Puck TT (1989): Genome exposure in reverse transfor- mation. 29th Annual Meeting of the American Society for Cell Bi- ology, Houston, TX, November 5-9. J Cell Biol109:231a (Abstract).

Law ML, Xu YW, Gao JZ, Puck TT (1989): A human repetitive se- quence behaves as a regulatory element. ERICR Scientific Poster Session, Denver, CO, January 17. (Abstract).

Law ML, Gao J , Puck TT (1989): A nuclear protein associated with hu- man cancer cells binds preferentially to a human repetitive DNA sequence. American Society of Human Genetics 40th Annual Meeting, Baltimore, MD, November 11-15. Am J Hum Genet 45: A26 (Abstract).

Law ML, Gao J , Puck TT (1989): A nuclear protein associated with hu- man cancer cells binds preferentially to a human repetitive DNA sequence. Proc Natl Acad Sci USA 863472-8476,

Matsukura M, Willey J , Miyashita M, Taffe B, Waldren C, Puck TT, Harris CC (1989): Detection of direct mutagenicity of cigarette smoke condensate in the AL hybrid cell mutagenesis assay. Amer- ican Association for Cancer Research Annual Meeting, San Fran- cisco, CA, May 24-27. Proc Am Assoc Canc Res 30:145 (Abstract).

Miranti C, Evans R, Puck TT (1989): Protein synthesis and phospho- rylation resulting from treatment of CHO-K1 cells with CAMP de- rivatives. The Fourth Aspen Cancer Conference, Aspen, CO, July 23-26. Toxicol Pathol 17:830-831 (Abstract).

Puck TT (1989): The teaching of science in a democracy. (UnPub- lished).

Puck TT, Miranti C (1989): Reverse transformation, the cytoskeleton and genome regulation. The Fourth Aspeen Cancer Conference, Aspen, CO, July 23-26. Toxicol Pathol 172335 (Abstract).

Puck TT (1989): The effects of radiation on the immunohematopoietic system. American College of Physicians, Colorado Chapter Scien- tific Meeting, Colorado Springs, CO, January 19-21. (Abstract).

Puck TT (1989): Mammalian cell genetic regulation and the nature of cancer. In: “A Memorial Colloquium Honoring Herbert L. Ander- son, August 31, 1988, Los Alamos National Laboratory.” Los Alamos, NM: Los Alamos National Laboratory, pp 9-15.

Waldren C, Bedford J , Hei T, Parker R, Puck T (1989): Mutation on studies in human-hamster hybrid (A,) cells. In “Radiation Re- search Society 37th Annual Meeting.” Seattle, WA, March 18-23. (Abstract).

Waldren C, Harvey W, Snead D, Webb P, Puck T (1989): Mutagenesis and human disease. In “ERICR Scientific Poster Session.” Denver, CO, January 17 (Abstract).

Waldren C, Puck TT (1989): Measurement of mutagenesis by exhaust chemicals using the AL cell system. In “Sixth Health Effects Insti- tute Annual Conference.” Cambridge, MA, April 8-12 (Abstract).

Bunn PA, Dienhart DG, Chan D, Puck TT, Tagawa M, Jewett PB, Braunchweiger E (1990): Neuropeptide stimulation of calcium flux in human lung cancer cells: Delineation of alternative pathways. Proc Natl Acad Sci USA 87:2162-2166.

Dienhart DG, Chan D, Puck TT, Tagawa M, Jewett PB, Braunch- weiger E, Bunn PA (1990): Multiple neuropeptides increase intra- cellular calcium concentrations via separate but related second messenger pathways in small cell lung cancer. Annual Meeting Western Student Medical Research Committee, Carmel, CA, Feb- ruary 5-9 (Abstract).

Haag MM, Halle K, Krystosek A, Arenson E, Puck TT (1990): Growth control and genome exposure in neuroectodermal cell lines. Inter- national Pediatric Neuro-Oncology Symposium, Philadelphia, PA, May 24-26 (Abstract).

Krystosek A, Puck TT (1990): The spatial distribution of exposed nu- clear DNA in normal cancer, and reverse-transformed cells. Proc Natl Acad Sci USA 87:6560-6564.

Miranti C, Puck TT (1990): Gene regulation in reverse transforma- tion: Cyclic AMP-induced actin homolog in CHO cells. Somat Cell Mol Genet 16:67-787.

Puck TT, Krystosek A, Chan D (1990): Genome regulation in mam- malian cells. Somat Cell Mol Genet 16:257-265.

Waldren C, Sinclair J , Wood S, Puck T, Webb P, Doolittle D, Smith C, Ueno A (1990): Mutation analysis by AJprimary embryonic chick hepatocyte (AJpech) method: Application to mutagens in cooked food. In “Environmental Mutagen Society Annual Meeting.” Albu- querque, NM, March 25-29 (Abstract).

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Matsukura N, Willey J , Miayashita M, Taffe B, Hoffman D, Waldren C, Puck TT, Harris CC (1991): Detection of direct mutagenicity of cigarette smoke condensate in mammalian cells. Carcinogenesis 12:685-689.

Puck TT, Krystosek A (1991): The role of genome exposure in cancer. In “The Sixth Aspen Cancer Conference.” Aspen, GO, July 21-25 (Abstract).

Puck TT, Bartholdi M, Krystosek A, Johnson R, Haag MM (1991): Confocal microscopy of genome exposure in normal, cancer, and re- verse transformed cells. Somat Cell Mol Genet 17:489-503.

Shibuya M, Fox M, Tatsumi K, Puck T, Bell-Prince C, Waldren C (1991): Flow cytometric analysis of mutations in genes for human cell surface antigens. In “XV Congress of the International Society for Analytical Cytology.” Bergen, Norway, August 25-30 (Abstract).

Waldren CA, Harvey WF, Puck TT, Ueno A, Doolittle DJ, Smith CJ, Wood S, Sinclair JF (1991): Quantitation of mutation induced in a human gene (al) of AL human-hamster hybrid cells by 3-methyl- cholanthrene activated by cocultivated chick hepatocytes: AJPECH assay system. Cancer Res (In Press).

Gurley LR, Jandacek AL, Valdez JG, Puck TT (1992): Cyclic AMP in- duction of apoptosis in synchronized CHO cells. In “32nd Annual Meeting of the American Society for Cell Biology.” Denver, CO, November 15-19 (Abstract).

Krystosek A, Griffin P, Puck TT (1992): Cancer diagnostic signifi- cance of a new DNA-labeling method. In “Fifth Annual Can- cer Biotechnology Symposium.” Fort Collins, CO, September 22 (Abstract).

Puck TT, Johnson R, Krystosek A (1992): Functional interpretation of interphase chromosome domains. In “American Society for Cell Biology Thirty-Second Annual Meeting.” Denver, CO, November 15-19 (Abstract).

Puck TT, Krystosek A (1992): The role of the cytoskeleton in genome regulation and cancer. Int Rev Cytol 132:75-108.

Puck TT, Krystosek A (1992): Genome exposure in cancer. In “Fourth Annual Structural Biology Symposium.” Santa Fe, NM, April 24 (Abstract).

Puck TT, Krystosek A, Johnson R (1992): The genome exposure defect in cancer. 42nd Annual Meeting of the American Society of Human Genetics, San Francisco, CA, November 9-13. Am J Hum Genet 51S:A279 (Abstract).

Gurley LR, Jandacek AL, Valdez JG, Puck TT (1993): Br-cAMP in- duction of apoptosis in synchronized CHO cells: Potential implica- tions concerning beta agonists. In “32nd Annual Meeting of the So- ciety of Toxicology.” New Orleans, LA, March 14-18 (Abstract).

Puck TT, Krystosek A (1993): Reverse transformation, genome expo- sure, and cancer. In Vande Woude GF, Klein G (eds): “Advances in Cancer Research.” San Diego: Academic Press, Inc., pp 125-151.

Puck TT (1993): Mutation measurement and the prevention of cancer. In “Research Professors’ Meeting of the American Cancer Society.” Santa Cruz, CA, April 1 4 1 6 (Abstract).

Puck TT, Morse H, Johnson R, Waldren CA (1993): Caffeine enhanced measurement of mutagenesis by low levels of gamma-irradiation in human lymphocytes. Somat Cell Mol Genet 19:423429.

Puck TT, Krystosek A, Marcom K, Johnson R, Webb P, Griffin P (1993): Cancer and the genome exposure reaction. DOE’S Human Genome Workshop, Santa Fe, NM, February 7-11 (Abstract).

Puck TT (1993): Genomic instability 11. In “Eighth Aspen Cancer Con- ference.” Aspen, CO, July 18-22 (Abstract).

Haag MM, Krystosek A, Arenson E, Puck TT (1994): Reverse Trans- formation and Genome Exposure in the C6 Glial Tumor Cell Line. Cancer Invest 12:3345.

Puck TT (1994): Living History Biography. Am J Med Genet (in press). Puck TT (1994): The cytoskeleton and supporting structures: Oppor-

tunities for drug development in cancer. Cancer Progress 94 Con- ference, New York, NY, February 28-March 1 (Abstract).