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Intra-host speciation in the

Lamellodiscus ignoratus

morphotype?

Timothe Poisot Olivier Verneau Yves Desdevises

Universit de Montpellier Facult des Sciences

August 2008

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Monogeneans : gill parasites

Haptor

Anterior

sucks

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Monogeneans : gill parasites

Eggs

Larvae Activ

eho

stsearch

Haptor

Anterior

sucks

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Evolution ofLamellodiscus

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Evolution ofLamellodiscus

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Evolution ofLamellodiscus

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Evolution ofLamellodiscus

Cospeciation + intra-host speciation + ?3

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Hypotheses forLamellodiscus evolution

Host-switch followed by speciation

Suggested for Gyrodactylus

Thought important for Lamellodiscus

Intra-host speciation

Suggested for Dactylogyrus

Yet to study for Lamellodiscus

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Methods

Haptor (fixation organ)

18S 5.8S[]

ITS1

tRNA-Trp Cox1 tRNA-Thr 16S

Variable in size (1kb)

Desdevises et coll. 2000

Littlewood et coll. 1997

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Why the ignoratus morphotype?

L. ignoratus

L. neifari

L. confusus

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Why the ignoratus morphotype?

L. ignoratus

L. neifari

L. confusus

6 5 4 3 2 1 0 1 2 3 4

2.0

1.5

1.

0

0.5

0.0

0.5

1.0

1.5

2.0

2.5

Axe 1: 71.1 %

Axe2:8.5

4

%

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Genetic similarity (%)

18S Its1 Cox1

L. ignoratus

L. neifari

Ignoratus-like

95,7 86 76

99,8 85 91

97,3 86 79

18S Mouse Chicken Xenopus Nematode

Human 99,2 96,4 95,9 87,1

18S

Mean ( species) : 94.3%L. fraternus/L. ergensi: 99.4%

L. ignoratus/L. virgula : 94.3%

L. parisi/L. elegans : 90.8%

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18S rDNA (Maximum likelihood + Bayesian)

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18S rDNA (Maximum likelihood + Bayesian)

Ignoratus

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( )

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Its1 (ML + Bayesian)

?

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I 1 (ML B i )

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Its1 (ML + Bayesian)

Ignoratus

?

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H th

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Hypotheses

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H th

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Hypotheses

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H th

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Hypotheses

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C l i

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Conclusions

Genetic variability of Lamellodiscus is high

Confirmed by new sequences (Cox1)

Potential under-estimation of the real diversity of the genus

Theignoratus morphotype

New species are not genetically distinguishable

Paraphyletic species, or greater diversitythan expected?

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Thank you for your attention

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C 1 ti ti f t ti

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Cox1 : estimation of saturation

0.00 0.05 0.10 0.15 0.20 0.25

0.0

0.1

0.2

0.3

0.4

Ti

Tv

0.00 0.05 0.10 0.15 0.20 0.25

0.0

0.1

0.2

0.3

0.4

Ti

Tv

0.00 0.05 0.10 0.15 0.20 0.25

0.0

0.1

0.2

0.3

0.4

Ti

Tv

Triplet 1 & 2 3

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Principal components anal sis

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Principal components analysis

!6 !5 !4 !3 !2 !1 0 1 2 3 4

!2.5

!1.0

0.5

1.5

L. elegans

Axe 1: 74.5 %

Axe2:9.2

8%

!

!!

!8 !7 !6 !5 !4 !3 !2 !1 0 1 2 3

!2.5

!1.0

0.5

1.5

L. ergensi

Axe 1: 66.7 %

Axe2:10.7%

!

!

!!6 !5 !4 !3 !2 !1 0 1 2 3 4

!2.0

!0.5

1.0

2.0

L. ignoratus

Axe 1: 71.1 %

Axe2:8.5

4%

!4 !3 !2 !1 0 1 2 3 4 5

!2.5

!1.0

0.5

2.0

L. kechemirae

Axe 1: 54.5 %

Axe2:15.3%

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Ignoratus like

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Ignoratus-like

L. neifari

L. falcus

L. ignoratus