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Elucidating the identity and physiology of sulfate-reducing microorganisms in the hidden sulfur cycle of freshwater habitats Dissertation zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften (Dr.rer.nat) vorgelegt von Susanne Wörner an der Mathematisch- Naturwissenschaftliche Sektion Fachbereich Biologie Konstanz, 2019 Konstanzer Online-Publikations-System (KOPS) URL: http://nbn-resolving.de/urn:nbn:de:bsz:352-2-1ctholi8hx1nr9

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Page 1: Elucidating the identity and physiology of sulfate

Elucidating the identity and physiology of sulfate-reducing

microorganisms in the hidden sulfur cycle

of freshwater habitats

Dissertation zur Erlangung des

akademischen Grades eines

Doktors der Naturwissenschaften (Dr.rer.nat)

vorgelegt von

Susanne Wörner

an der

Mathematisch- Naturwissenschaftliche Sektion

Fachbereich Biologie

Konstanz, 2019

Konstanzer Online-Publikations-System (KOPS) URL: http://nbn-resolving.de/urn:nbn:de:bsz:352-2-1ctholi8hx1nr9

Page 2: Elucidating the identity and physiology of sulfate

Tag der mündlichen Prüfung: 24. Juli 2019

1. Referent: Prof. Dr. Bernhard Schink

2. Referent: Prof. Dr. Michael Pester

Page 3: Elucidating the identity and physiology of sulfate

Danksagung

Zuallerst möchte ich meinem Betreuer Prof. Dr. Michael Pester für die Möglichkeit danken, an

diesen unterschiedlichen und sehr interessanten Projekten zu arbeiten, sowie für die zahlreichen

konstruktiven Diskussionen, Ratschläge und unermüdliche Unterstützung während meiner

Arbeit.

Besonderer Dank gilt auch Prof. Dr. Bernhard Schink, für die Möglichkeit am Lehrstuhl für

Limnologie und Mikrobielle Ökologie diese Arbeit durchführen zu können und für die vielen

lohnenden Ideen und Diskussionen.

Ich möchte mich auch bei Herrn Prof. Dr. Andreas Kappler für die Übernahme des

Prüfungskomitees bedanken.

Bei Sylke Wiechmann möchte ich mich für ihre selbstverständliche Hilfe bei jeglichen Fragen

und Problemen bedanken.

Allen anderen Mitarbeitern des Lehrstuhls Schink und der Arbeitsgruppe Schleheck danke ich

für ihre Unterstützung und Hilfsbereitschaft, sowie der nicht endenden Versorgung mit Kuchen.

Ferner möchte ich allen Studenten, die mich während meiner Arbeit begleitet haben danken,

ganz besonders Evgenia Isaeva und Dominik Stickel, die mir während ihrer Bachelorarbeiten

eine große Hilfe waren.

Meiner Familie und Freunden, besonders Walter, Sebastian, Matthias und Diana möchte ich

dafür danken, dass sie mir in jeglicher Lage den Rücken stärkten.

Ganz besonders möchte ich mich bei Jochi für die unendliche Unterstützung, Ermutigung und

Motivation bedanken.

Page 4: Elucidating the identity and physiology of sulfate

Table of Content

Chapter 1 General Introduction

Freshwater habitats – lakes and wetlands 1

Polymer degradation in anoxic environments 2

Sulfur cycle in freshwater habitats 4

Chapter 2 Gypsum amendment to rice paddy soil stimulated bacteria involved in sulfur

cycling but largely preserved the phylogenetic composition of the total bacterial

community

Abstract 8

Introduction 8

Results and Discussion 10

Supplementary Information 19

Chapter 3 The microbial succession of anaerobic chitin degradation in freshwater sediments

Abstract 20

Introduction 21

Results 23

Discussion 31

Materials and Methods 34

Supplementary Information 39

Chapter 4 The active sulfate-reducing microbial community in littoral sediment of

oligotrophic Lake Constance

Abstract 61

Introduction 62

Results 64

Discussion 72

Materials and Methods 75

Supplementary Information 79

Chapter 5 General Discussion and Outlook 80

Chapter 6 Summary 85

Chapter 7 Zusammenfassung 87

Chapter 8 Record of Achievement 89

References 90

List of Publications 107

Page 5: Elucidating the identity and physiology of sulfate

General Introduction

1

Chapter 1

General Introduction

Freshwater habitats - lakes and wetlands

Freshwater habitats, including limnic and wetland (rice fields) environments, are important

ecosystems in the global carbon cycle (Cole et al., 2007). Natural (swamps, peat, bogs, marches)

and human-made (rice fields) wetlands cover 6% of the Earth´s surface (Junk et al., 2013), of

which 0.9 Pg CO2 and 0.2 Pg CH4 are released annually into the atmosphere (Mitsch, 2016). At

the same time, wetlands sequester CO2 while burying and storing organic carbon in their

sediments and act as carbon sink (Duarte and Prairie, 2005). These terrestrial environments are

permanently or only temporarily covered with water and follow therefore oxic and anoxic

conditions, to which the microbial community has to adapt as response to these changing

environmental conditions (Brune et al., 2000). As far as the soil is covered with water, a distinct

layering of oxic and anoxic zones emerge with steep redox conditions as in anoxic sediments.

In addition to these temporal or seasonal oxygen changes, plant vegetation provides micro-oxic

zones in the anoxic soil on the rhizosphere by their aerenchyma (Jackson and Armstrong, 1999)

that is necessary for root respiration (Brune et al., 2000; Ström et al., 2005). This enables oxic

and anoxic turnover processes on the rhizosphere as well as the re-oxidation of reduced terminal

electron acceptors and the oxidation of CH4 by aerobic methanotrophic bacteria (Brune et al.,

2000; Dedysh, 2011). The vegetation of wetlands also highly contributes to the carbon flow

within these ecosystems either by dead plant material (cellulose) or by photosynthesized carbon

that is released as root exudates on the rhizosphere. These released compounds can count for

up to 25% of their net fixed carbon (Jones et al., 2009). Most of these root exudates are soluble

compounds, such as sugars, amino acids, short chain fatty acids, phenolic compounds that can

directly be used by microorganisms as carbon and energy source in these habitats (Bais et al.,

2006). The high impact of root exudates alters the microbial communities, inhabiting the

rhizosphere and leads even to distinct microbial communities on the endosphere, rhizoplane

and rhizosphere that are different to the bulk soil (Edwards et al., 2015).

Lakes cover 3.7% of the Earth´s surface (Verpoorter et al., 2014) and are, in contrast to most

wetlands, permanently covered with water and comprise a waterfilled sediment, resulting in

more constant environmental conditions of aerobic or anaerobic degradation processes of

organic matter. CH4 released from lakes contribute between 6 and 36 Tg carbon annually

Page 6: Elucidating the identity and physiology of sulfate

General Introduction

2

(Bastviken et al., 2004) and 0.11 Pg carbon by CO2 (Cole et al., 2007). Carbon availability is

limited in most freshwater habitats and dissolved organic and inorganic carbon (DOC and DIC)

are the major carbon inputs, followed by particulate organic carbon (POC) (Tranvik, 2009). In

addition, primary production of microalgae and phytoplankton can seasonally contribute to the

carbon intake mainly in the water column (Weisse et al., 1990). For anerobic microorganisms

within the sediment, carbon availability is much more limited and depends mainly on the

sedimentation of organic particles such as dead organisms, larval cases or the shells of

crustaceans after ecdysis (Kolmakova et al., 2019) . Soluble organic compounds are most likely

readily used by the aerobic microbial community, leaving complex organic compounds as the

main available carbon source to the microbial community in the anoxic sediment. Little is

known about the anaerobic polymer-hydrolyzing microbial community in Lake Constance

sediment although it is the major step of organic matter degradation.

Polymer degradation in anoxic environments

The degradation of polymeric compounds needs the initial hydrolysis of branched and complex

structures to linear compounds that can be shortened to oligomers and imported into cells to get

excess to the soluble compounds. In the absence of oxygen, the formation of energy (ATP) is

the most limiting factor during substrate use, resulting in specialists of distinct pathways of a

restricted number of substrates. To decomplexify organic polymeric compounds, the enzymatic

activity of endoenzymes is necessary that randomly cleave the polymer, leading to linear

structures of different length. This metabolic trait is not widespread in anaerobic

microorganisms and is found mainly within the Clostridia (Bayer et al., 2004). The ability to

use oligomeric compounds is much more wide-spread and serve as direct substrate by importing

the oligomers into the cell or by the sequential hydrolysis of mono- or dimeric structures by

exoenzyme activities. So far, there is no defined terminology of polymer-hydrolyzing

microorganisms that either can hydrolyze complex organic compounds or these

microorganisms that solely metabolize oligomeric structures (Figure 1), which cannot not use

complex compounds in the absence of polymer-hydrolyzing members or those that profit from

the depolymerization of hydrolytic microorganisms while using oligomeric compounds. By

contrast, polymer-hydrolyzing microorganisms could short-cut the degradation pathway

depicted in Figure 1 and can perform step 1 and 2 in a combined reaction (Sorokin et al., 2014).

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General Introduction

3

In general, all polymer-hydrolyzing microorganisms can use the downstream compounds

(oligomers, dimers, monomers) and the direct import of oligomeric structures is more common

in anaerobic microorganisms than the complete hydrolysis outside the cell (Li and Roseman,

2004). Polymer depolymerization is mediated mainly by fermenting microorganisms (e.g.

Clostridia) and gain energy (ATP) primarily by substrate level phosphorylation, while these

microorganisms disproportionate energy-rich organic compounds (Thauer et al., 1977).

Resulting fermentation products differ between species and the environmental conditions.

Typical fermentation products are short-chained organic acids (butyrate, propionate, lactate,

succinate, acetate), alcohols (e.g. EtOH), H2, formate and CO2 that are released in the

surrounding environment. Depending on the prevalent hydrogen concentration (partial

pressure), fermentation products vary within one species, while these microorganisms have to

switch to alternative pathways which are more favorable (Schink, 1997). In an active and intact

microbial food web, with low carbon intake and a fast turnover (scavenge) of H2, many primary

fermenters can change their pathway to an almost exclusive acetate/CO2/H2 production and

profit by a higher energy yield than by the release of longer organic acids. Secondary fermenters

can use the more energy rich fermentation products (e.g. succinate, EtOH, lactate) directly

Figure 1: Degradation steps during anaerobic polymer turnover under fermentative and

methanogenic conditions. 1: Polymer-hydrolyzing microorganisms, 2: Saccharolytic primary

fermenter, 3: Secondary fermenters, 4: Methanogens, 5. Homoacetogens. Modified from

Schink (1997).

Page 8: Elucidating the identity and physiology of sulfate

General Introduction

4

(Schink et al., 1987), whereas the oxidation of propionate or butyrate is an endergonic reaction

under standard conditions and needs therefore the cooperation of a syntrophic partner that keep

the H2 pool low (Müller et al., 2010). Fermentation products of secondary fermenters are mainly

acetate, H2-CO2 or formate. In the absence of alternative electron acceptors, methanogenesis is

the terminal mineralization step using either H2 -CO2 or acetate (Conrad, 1999). In the presence

of alternative electron acceptors, anaerobic respirations can occur, following a sequential

reduction chain in which electron acceptors with a higher redox potential are reduced first: O2,

NO3-, Mn(IV), Fe(III), SO4

2-, CO2 (Peters and Conrad, 1996). In Lake Constance sediment,

methanogenesis was reported to be the major terminal mineralization step during carbon

degradation (Thebrath et al., 1993). But the high sulfate reduction rates, occurring in this

habitat, indicated a high impact of sulfate reduction during anaerobic carbon turnover as well

(Bak and Pfennig, 1991a).

Sulfur cycle in freshwater habitats

Sulfur is an important element of all living species and accounts for approximately 1% of its

dry weight, where it serves as structural and enzymatic function in amino acids and many

enzymes, respectively. In nature, sulfur compounds are present in different oxygenated forms

from the most oxidized sulfate (+6) to sulfide (-2) as the most reduced form (Lucheta and

Lambais, 2012), (Figure 2). Oxidation of reduced sulfur species can occur by chemical reactions

with oxygen or by biological microbial processes of sulfur-oxidizing microorganisms to

elemental sulfur, thiosulfate or sulfate (Ghosh and Dam, 2009). Sulfide is oxidized by

chemolithotrophic sulfur-oxidizing microorganisms (e. g. Thiobacillus) under oxic or by

phototrophic microorganisms (e.g. Chromatium) under anoxic conditions, to sulfur or sulfate

(Vishniac and Santer, 1957; Trüper et al., 1982). Elemental sulfur can be either reduced back

to sulfide by e.g. Desulfurmonas (Gebhardt et al., 1985) or oxidized to sulfate (Trüper et al.,

1982). The oxidation of sulfide to thiosulfate can be mediated by Cyanobacteria during

anaerobic photosynthesis of sulfide (de Wit and van Gemerden, 1987). Photo- and

chemolithotrophic Alphaproteobacteria oxidize thiosulfate to sulfate (e.g. Paracoccus)

(Friedrich et al., 2005). In addition, thiosulfate, elemental sulfur and sulfite can also be

disproportionated to sulfate and sulfide (e.g. Desulfovibrio) (Canfield and Thamdrup, 1994;

Habicht et al., 1998). Sulfite, however, is a central intermediate during dissimilatory sulfate

reduction, which is further reduced to sulfide via the dissimilatory sulfite reductase (dsr) in

sulfate-reducing microorganisms (SRM). Different Proteobacteria and Chlorobi use this

Page 9: Elucidating the identity and physiology of sulfate

General Introduction

5

enzyme in reverse (rdsr), while these microorganisms oxidize sulfide to sulfite (Loy et al.,

2009).

Sulfate reduction occurs either by an assimilatory reaction, where sulfate is metabolized to use

it as sulfur source or by the dissimilatory sulfate reaction cascade, in which energy is conserved

during anaerobic respiration. These sulfate-reducing microorganisms (SRM) can be found

within the bacterial lineages Deltaproteobacteria, Clostridia, Nitrospirae,

Thermodesulfobiaceae, Thermodesulfobacteria and the archaeal phyla Euryarchaeota and

Crenarchaeota (Muyzer and Stams, 2008). However, although SRM are represented by

different phylogenetic lineages, all known SRM share a conserved pathway of sulfate reduction,

including ATP-sulfurylase (sat), adenylyl-sulfate reductase (apr) and dissimilatory sulfite

reductase (dsr) (Rabus et al., 2006). This conserved pathway enabled the use of the

dissimilatory (bi-)sulfite reductase (dsrAB) as phylogenetic marker gene for SRM, and reveled

Figure 2: Shown is a selection of most important pathways of microbial processes within the

sulfur cycle. Oxidation state of respective sulfur compounds are given in brackets. For

thiosulfate, the oxidation states of the two sulfur are given separately. Modified from Pester et

al. (2012) .

Page 10: Elucidating the identity and physiology of sulfate

General Introduction

6

in a comprehensive study of this dsrAB gene sequence at least 13 novel uncultured dsrAB

lineages without cultured representatives (Pester et al., 2012; Müller et al., 2015). Two of these

uncultured family-level dsrAB lineages, uncultured family-lineage 8 and 13, could recently be

affiliated to the phyla Acidobacteria and Nitrospirae, respectively (Hausmann et al., 2018;

Zecchin et al., 2018). Additional metagenome analyses revealed the genetic capacity of sulfate-

or sulfite reduction in phyla that were so far not considered as members of the SRM, e.g.

Chloroflexi, Armatimonadetes, Ignavibacteria and Verrucomicrobia (Wasmund et al., 2016;

Anantharaman et al., 2018).

Known sulfate-reducing microorganisms are highly versatile in their use of substrates and are

able to use diverse short chain fatty acids, ethanol, aromatic compounds and H2-CO2 (Muyzer

and Stams, 2008; Rabus et al., 2013). In marine environments, where high sulfate

concentrations of up to 28 mM or higher occurs, sulfate reduction was reported as an important

process during anaerobic carbon mineralization (Jørgensen, 1982). Freshwater habitats are

usually low in sulfate (µM range) and therefore considered as mainly methanogenic

environments. Despite these low sulfate concentrations, it was repeatedly observed that sulfate

reduction rates in freshwater habitats were as high as in marine sediments (Bak and Pfennig,

1991a; Pester et al., 2012). This lead to the conclusion that the low sulfur pool had to undergo

a fast turnover of reduced and oxidized states in these habitats (Pester et al., 2012). The ongoing

sulfate reduction in these habitats showed that sulfate never depleted and that the reduced sulfur

species have to be re-oxidized as fast as sulfate is reduced, under both oxic and anoxic

conditions. The authors proposed the presence of a cryptic sulfur cycle within these habitats,

where reduced sulfur compounds are re-oxidized by the parallel reduction of either oxygen in

oxic or the reduction of iron(III), humic acids (Quinones) or nitrate in anoxic habitats (Figure

2) (Pester et al., 2012). This theoretical model has not been investigated so far and little is

known about the involved microbial communities during the reductive and oxidative branch of

this cycle.

Aims of this dissertation

In this study, the reductive part of the cryptic sulfur cycle was investigated in more detail. Here,

we wanted to understand the contribution of SRM during natural occurring carbon cycles in

different freshwater habitats – rice paddy soil and lake sediment. In both habitats, we wanted

to understand the prevailing carbon sources for SRM and investigated therefore the carbon flow,

including fermentation products and released gases. In rice fields, carbon flow was investigated

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General Introduction

7

by photosynthesized CO2 that was released as root exudates and in Lake Constance sediment

by the anaerobic degradation pathway of chitin in sediment slurries. In both environments, the

SRM community was investigated by next generation sequencing methods to detect the native

occurring conditions and SRM community in relation to SRM alterations during carbon flow

under as far as possible natural conditions.

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Chapter 2

8

Chapter 2

Gypsum amendment to rice paddy soil stimulated bacteria involved in

sulfur cycling but largely preserved the phylogenetic composition of the

total bacterial community

Susanne Wörner, Sarah Zecchin, Jianguo Dan, Nadezhda Hristova Todorova, Alexander Loy,

Ralf Conrad, Michael Pester

Published in Environmental Microbiology Reports (2016) 8(3), 413-423.

Abstract

Rice paddies are indispensable for human food supply but emit large amounts of the greenhouse

gas methane. Sulfur cycling occurs at high rates in these water‐submerged soils and controls

methane production, an effect that is increased by sulfate‐containing fertilizers or soil

amendments. We grew rice plants until their late vegetative phase with and without gypsum

(CaSO4·2H2O) amendment and identified responsive bacteria by 16S rRNA gene amplicon

sequencing. Gypsum amendment decreased methane emissions by up to 99% but had no major

impact on the general phylogenetic composition of the bacterial community. It rather selectively

stimulated or repressed a small number of 129 and 27 species‐level operational taxonomic units

(OTUs) (out of 1883–2287 observed) in the rhizosphere and bulk soil, respectively. Gypsum‐

stimulated OTUs were affiliated with several potential sulfate‐reducing (Syntrophobacter,

Desulfovibrio, unclassified Desulfobulbaceae, unclassified Desulfobacteraceae) and sulfur‐

oxidizing taxa (Thiobacillus, unclassified Rhodocyclaceae), while gypsum‐repressed OTUs

were dominated by aerobic methanotrophs (Methylococcaceae). Abundance correlation

networks suggested that two abundant (>1%) OTUs (Desulfobulbaceae, Rhodocyclaceae) were

central to the reductive and oxidative parts of the sulfur cycle.

Introduction

Rice agriculture provides food for more than 50% of the world's human population

(http://irri.org). At the same time, rice paddies are major sources of the greenhouse gas methane,

contributing 10%–15% of its annual emission to the atmosphere (Houweling et al., 1999;

Wuebbles and Hayhoe, 2002; Conrad, 2009). After soil flooding, a sequential activation of the

redox processes nitrate, iron, and then sulfate reduction can be typically observed with

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Chapter 2

9

fermentation coupled to methanogenesis being the last but long‐lasting degradation process to

become active (e.g., (Achtnich et al., 1995a; Yao et al., 1999; Liesack et al., 2000)). Within the

first days after flooding, sulfate concentrations in rice paddy soil typically decrease from 0.4–

5 mM down to steady state concentrations of 1–99 µM (Achtnich et al., 1995a; Wind and

Conrad, 1997; Yao et al., 1999; Lueders and Friedrich, 2000). However, even after 13 weeks

of flooding high sulfate reduction rates (SRR) were measured in the vicinity of rice roots (200–

500 nmol cm−3 d−1) and in anoxic bulk soil (20–97 nmol cm−3 d−1) (Wind and Conrad, 1997).

To explain these high SRRs, an intense re-oxidation of reduced sulfur back to sulfate has been

proposed (Freney et al., 1982; Lefroy et al., 1992; Wind and Conrad, 1997; Pester et al., 2012).

This sulfur cycling can occur at oxic‐anoxic interfaces such as rice roots and the upper soil

layers. However, it apparently runs also under anoxic conditions (Pester et al., 2012), where

reduced sulfur species may be re-oxidized with the help of iron minerals (Hansel et al., 2015)

or redox‐active parts of humic material such as quinone moieties (Heitmann and Blodau, 2006;

Yu et al., 2015) as shown for other freshwater habitats.

Sulfate reduction is energetically more favorable than fermentative processes and

methanogenesis (Muyzer and Stams, 2008). As such, sulfate amendment to rice paddy fields

has been suggested as one of several strategies to mitigate methane emissions (Schütz et al.,

1989; Lindau et al., 1993; van der Gon and Neue, 1994; Yagi et al., 1997; van der Gon et al.,

2001). In addition, sulfate can be applied to rice paddy fields to meet the biological S demand

of rice plants in form of the common fertilizers (NH4)2SO4 and phosphogypsum or as soil

additive to sodic and/or alkaline soils in form of gypsum (CaSO4·2H2O). Described application

rates of such sulfate amendments range from 0.5–6.7 Mg ha−1 and were leading to a reduction

of methane emissions by 10%–71% (van der Gon et al., 2001). At the same time, there was no

reported adverse effect on rice growth (van der Gon et al., 2001) or yield (Wassmann et al.,

2000) by produced H2S, which was likely either immediately re‐oxidized to sulfate within the

oxidative branch of the sulfur cycle or immobilized in the fractions of acid volatile sulfide

(mainly FeS) or chromium reducible sulfide (mainly FeS2) (Wind and Conrad, 1995; Wind and

Conrad, 1997). In addition, several million hectares of rice paddy soils are acid sulfate soils and

(coastal) saline soils with naturally high sulfate contents (van der Gon et al., 2001). The effect

mediated by increased soil sulfate contents is predicted to become even more pronounced

because human population growth coupled to increasing energy consumption in Asia and other

regions is predicted to increase global sulfur deposition on rice paddies by acid rain (Gauci et

al., 2004; Gauci et al., 2008).

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10

Previous work identified sulfate‐reducing microorganisms (SRM) belonging to the

Desulfobacteraceae, Desulfobulbaceae, Desulfovibrionaceae, Syntrophobacteraceae and

Peptococcaceae in rice paddy soil using cultivation, 16S rRNA gene‐targeted quantitative PCR,

and metaproteogenomic approaches (Wind and Conrad, 1995; Wind et al., 1999; Scheid and

Stubner, 2001; Stubner, 2002; Scheid et al., 2004; Stubner, 2004; Lu et al., 2006; Knief et al.,

2011). Isolation of rice paddy SRM on a wide range of substrates including H2/CO2, acetate,

propionate, butyrate, iso‐butyrate, caproate, pimelate, acetone and benzoate (Wind et al., 1999)

indicated their potential role in the turnover of organic matter degradation intermediates. Less

is known about sulfur‐oxidizing microorganisms in rice paddy soil. Cultivation‐based

approaches have identified facultative chemolithoautotrophic Alphaproteobacteria (genera

Ancylobacter, Xanthobacter and Bosea) and obligately chemolithoautotrophic

Betaproteobacteria (Thiobacillus) to be able to oxidize various reduced sulfur species (FeS, S0,

S2 , S4 ) in rice paddy soil (Stubner et al., 1998); with a high potential for thiosulfate

consumption of up to 1.9 µmol cm−3 d−1 (Wind and Conrad, 1995).

In this work, we show for the first time the impact of sulfate amendment to the overall bacterial

soil community in an intact soil‐rice plant system. In addition, we provide evidence for the in

situ activity of previously identified sulfate reducing and sulfur oxidizing bacteria under the

applied conditions and tested for their dependence on plant root exudates. To discern the effect

of sulfate only, we chose gypsum as a commonly applied soil additive and compared its effect

to unamended controls.

Results and Discussion

Gypsum amendment to paddy soil strongly decreased methanogenesis

Rice paddy microcosms were established in the greenhouse at a natural day‐night cycle (Frenzel

et al., 1992) for 58–59 days until the late vegetative phase of rice plants (Supporting Information

Fig. S1). The flooded soil was separated into a rhizosphere and bulk soil compartment by a 24-

µm mesh bag that prevented root penetration. The rhizosphere included both micro‐oxic and

anoxic sites as O2 typically diffuses in the mm‐scale from the rice root into the soil, whereas

the bulk soil was completely anoxic (Gilbert and Frenzel, 1998). To stimulate microorganisms

involved in sulfur cycling within rice paddies, soil in half of the microcosms was supplemented

with 0.15% (w/w) gypsum (CaSO4·2H2O) before flooding and rice seeding. Monitoring of

biogeochemical parameters in the soil porewater and in the headspace above microcosms

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11

started after rice roots were considered to fill a considerable part of the rhizosphere

compartment (day 35) and continued until the end of the experiment (day 59). Porewater sulfate

concentrations in gypsum amended microcosms were on average 3.5 and 2.6 mM in the

rhizosphere and bulk soil at day 35 and gradually decreased over time to 1.8 and 0.5 mM at day

59, respectively (Supporting Information Fig. S2A). Besides active sulfate turnover, this likely

mirrors a partial flow of sulfur from active sulfur (re)cycling into the largely immobilized

fractions of total reduced inorganic sulfur (e.g., FeS and FeS2) and carbon‐bound sulfur over

time (Wind and Conrad, 1997). In control microcosms not amended with gypsum, sulfate

concentrations fluctuated around 0.05 mM in the rhizosphere and were most often below the

detection limit in the bulk soil (Supporting Information Fig. S2A).

Gypsum amendment had a major impact on methane emission from rice microcosms. While in

control microcosms methane emission gradually increased from 1.4 mg m−2 h−1 at day 35 to 4.8

mg m−2 h−1 at day 59, it was drastically reduced in gypsum‐amended microcosms to an average

of 0.05 mg m−2 h−1 and showed no obvious increase or decrease over time (Supporting

Information Fig. S2B). This strong repression of methanogenesis was in accordance to previous

findings (Schütz et al., 1989; Lindau et al., 1993; van der Gon and Neue, 1994; Lindau et al.,

1998). The effect of gypsum was corroborated by methane measurements in soil porewater. In

control microcosms, average methane concentration of 487 and 102 µM were observed in the

bulk soil and rhizosphere, respectively, while the corresponding concentrations in gypsum‐

amended microcosms were 52 and 30 µM (Supporting Information Fig. S3A). Contrary to

methane, porewater concentration of carbon dioxide did not differ between different treatments

and fluctuated between 2.5 and 4.3 mM in the rhizosphere and 2.6 and 5.6 mM in the bulk soil

(Supporting Information Fig. S3B), indicating no adverse effect of gypsum on overall organic

matter mineralization.

Gypsum amendment largely preserved the bacterial community composition in rice paddy soil

To analyze the effect of gypsum on the total bacterial community, rhizosphere and bulk soil

samples were obtained from four microcosms per treatment at the end of the incubation. From

each soil sample, DNA was extracted in triplicates with each replicate being separately analyzed

by 454 amplicon sequencing of bacterial 16S rRNA genes (detailed in Supplementary

Information). In addition, the original soil inoculum was overlaid with water for three days and

thereafter analyzed in triplicates as well (denoted as T0). Species richness roughly doubled from

freshly flooded soil (1023 observed operational taxonomic units – OTUs – at 97% sequence

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identity) to established rhizosphere and bulk soil communities (1883–2287 OTUs). However,

there was no pronounced difference in species richness between the rhizosphere and bulk soil

communities and among the different treatments, with a maximum of 4906 and 7412 species‐

level OTUs as estimated by the Chao1 and ACE richness indices, respectively (Table S1).

Figure 1. Beta‐diversity of bacterial communities in rice plant microcosms. Separation of

bacterial communities in the analyzed soil habitats/treatments according to a principal

coordinate analysis (PCoA) based on the weighted unifrac metric and a non‐metric

multidimensional scaling (NMDS) analysis based on Bray–Curtis distances (stress = 0.06).

Individual points represent sequenced soil replicates, that is, three replicates obtained from four

different microcosms each per treatment (12 samples per treatment and soil compartment).

Freshly flooded soil was analyzed in triplicates only. Sampling depth was standardized using

the relative log expression (RLE) method as described by Anders and Huber (2010) and

implemented in the R package edgeR 3.6.8 (Robinson et al., 2010). PCoA and NMDS plots

were generated in the R package phyloseq 1.8.2 (McMurdie and Holmes, 2013).

Separation of bacterial community composition according to gypsum treatment was absent for

rhizosphere and bulk soil in a PCoA analysis based on weighted unifrac, which is a measure of

community dissimilarity that accounts for the phylogenetic relatedness of OTUs. On the

contrary, NMDS analysis based on Bray–Curtis distances, which ranks samples according to

OTU abundance only, revealed a clear effect of gypsum in the rhizosphere, whereas this effect

was less pronounced but still discernable for bulk soil samples (Fig. 1). In combination, both

analyses indicated that phylogenetically related OTUs lead to a separation in the NMDS plot,

while the general phylogenetic composition was preserved.

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Bacterial community composition was also clearly distinct between the rhizosphere, the bulk

soil, and the freshly flooded soil, which likely mirrors the different physicochemical parameters

shaping the three habitat types (Fig. 1). Major differences between the freshly flooded soil and

soils in the established microcosms were a higher relative abundance of Firmicutes (60%) and

Betaproteobacteria (20%) at T0 as compared with the rhizosphere and bulk soils (4%–12% and

6%–13%, respectively, Supporting Information Fig. S4). Rhizosphere and bulk soils mainly

differed in relative abundances of Alphaproteobacteria (7%–8% versus 4%),

Betaproteobacteria (12%–13% versus 6%–7%), Gammaproteobacteria (4%–6% versus 2%),

Acidobacteria (13%–15% versus 27%), Actinobacteria (1%–2% versus 4%–5%), Chloroflexi

(2% versus 3%–4%), and Cyanobacteria (8%–9% versus 1%–2%) (Supporting Information

Fig. S4).

Sulfate‐reducing Deltaproteobacteria responded strongest to gypsum amendment

To identify which OTUs were stimulated or repressed by gypsum, we tested for their

differential abundance in the treatments with and without gypsum using a general linear model

as implemented in the R package edgeR 3.6.8 (Robinson et al., 2010; Lund Steven et al., 2012).

In the rhizosphere, 66 OTUs were stimulated and 63 OTUs were repressed by gypsum spanning

at least 15 different bacterial phyla. The top ten positive responders were dominated by sulfate‐

reducing taxa, including two unclassified Desulfobulbaceae OTUs (OTU 0085 and 0738), two

Desulfovibrio OTUs (OTU 0575 and 0646), and two Syntrophobacter OTUs (OTU 0233 and

1363); all having a log2‐fold change in relative abundance of ≥2.9 (Fig. 2, Supporting

Information Table S2). Contrary, negative responders were dominated by methane‐oxidizing

Gammaproteobacteria including two Methylomonas OTUs (OTU 0271 and 0556), two

Methylobacter OTUs (OTU 0617 and 0747), one Methylosarcina OTU 0926, and two

Methylococcaceae OTUs (OTU 0212 and 1457); all having a log2‐fold change ≤–3.2

(Supporting Information Fig. 2 and Table S2). The observed decline of aerobic methanotrophs

was very likely linked to the decreased availability of methane under gypsum amendment as

their major energy substrate (Chistoserdova and Lidstrom, 2013).

To identify the subset of responding OTUs that strongly correlated in their abundance increase

upon gypsum treatment in each of the microcosms (in comparison to competitive exclusion or

neutral association), we performed an association network analysis. For the positive responders

in the rhizosphere, this revealed a separation into one large network and several smaller ones,

with the later consisting of 2–4 correlating response OTUs (Fig. 3). Most OTUs that were

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affiliated to sulfate‐reducing Deltaproteobacteria were part of the large response network.

Here, three unclassified Desulfobulbaceae OTUs (OTUs 0085, 0738, 2375) and one

unclassified Desulfobacteraceae OTU (OTU 1878) showed the highest connectivity (7–9 and

6 edges, respectively) to other OTUs and thus were situated in the center of the network. All

uunclassified Desulfobulbaceae OTUs shared Desulfobacterium catecholicum as their closest

cultured relative (95%–97% 16S rRNA identity, Supporting Information Fig. S5). D.

catecholicum can oxidize organic compounds completely to CO2 and has a very versatile

metabolism, ranging from the degradation of aromatic compounds and typical fermentation

intermediates including acetate to lithoautotrophic growth on H2 and CO2 (Szewzyk and

Pfennig, 1987). This metabolic versatility may explain the placement of related

Desulfobulbaceae OTUs at the center of the response network (Fig. 3). In contrast, OTUs

affiliated to Syntrophobacter (OTUs 0233, 3246, 1363, 1057), unclassified

Syntrophobacteraceae (OTUs 0066, 0122, 0737) and Desulfovibrio (OTUs 0646, 0572, 1282)

were situated rather in the periphery of the response network showing a lower degree of

correlating response to other OTUs. Syntrophobacter and Desulfovibrio spp. typically lack the

ability to utilize acetate and in the case of Syntrophobacter spp. often also H2/formate

(Wallrabenstein et al., 1994; Harmsen et al., 1998; Chen et al., 2005; Rabus et al., 2013).

However, both SRM taxa could be involved in the degradation of primary fermentation

intermediates since Desulfovibrio spp. are well adapted to degrade lactate and ethanol and

Syntrophobacter spp. are typical propionate degraders. Desulfovibrio spp. are also well known

to form stable syntrophic associations with other sulfate reducers and/or fermenters

(Wallrabenstein et al., 1994; Krumholz et al., 2015). Therefore, at least some of the responding

Desulfovibrio OTUs could also be involved in H2 or formate scavenging. This is supported by

previous work, which showed that sulfate reduction is coupled to H2 turnover in rice paddy soil

(Achtnich et al., 1995b).

At the very center of the rhizosphere response network, an unclassified Rhodocyclaceae OTU

(OTU 0019) was situated that was closely related to the sulfur‐oxidizing Sulfuritalea

hydrogenivorans (Kojima and Fukui, 2011) (97% 16S rRNA sequence identity, Supporting

Information Fig. S6) and showed a high connectivity to eight SRM‐affiliated OTUs (3

unclassified Desulfobulbaceae, 2 unclassified Syntrophobacteraceae, 2 Desulfovibrio, 1

unclassified Desulfobacteraceae). S. hydrogenivorans was described to utilize thiosulfate and

elemental S0 under anoxic conditions with nitrate as electron acceptor (Kojima and Fukui,

2011). The high relative abundance of the related Rhodocyclaceae OTU 0019 in the gypsum‐

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Figure 2. Taxonomic classification of 16S rRNA gene OTUs that significantly responded to

gypsum treatment in the rhizosphere and/or bulk soil compartment (FDR‐corrected P‐

value < 0.05). Each dot represents an OTU at the 97% identity level. Only OTUs that were

detected by at least 10 reads and were present in the rhizosphere or bulk soil of at least four

microcosms entered statistical analysis to discriminate against PCR and sequencing artifacts.

Sampling depth was standardized using the relative log expression (RLE) method as described

by Anders and Huber (2010) and implemented in the R package edgeR 3.6.8 (Robinson et al.,

2010). To test for differential abundance of OTUs between the different treatments, a general

linear model in combination with quasilikelihood shrunken dispersion estimates was applied as

implemented in edgeR (Lund Steven et al., 2012).

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treated rhizosphere (1.2 ± 0.4%) as compared to the control (0.18 ± 0.04%) (Supporting

Information Table S2) may further indicate a central role of this OTU in the oxidative branch

of rice paddy sulfur cycling. In addition, the two OTUs 0053 and 0623, both members of the

betaproteobacterial, sulfur‐oxidizing genus Thiobacillus (Muyzer et al., 2013), were identified

to respond positively towards gypsum. Thiobacillus spp. are known to oxidize a range of

reduced sulfur compounds (H2S, FeS2, S2 , polythionates) using O2 or as electron

acceptor (Subletta, 1987; Wood and Kelly, 1988; Drobner et al., 1992; Robertson and Kuenen,

2006; Bosch et al., 2012). In addition, two obligate chemolithoautotrophic Thiobacillus strains

that could utilize elemental S0, thiosulfate and tetrathionate were isolated from the same rice

paddy soil previously (Stubner et al., 1998). However, in contrast to Rhodocyclaceae OTU 0019

there was no strongly correlating abundance increase of SRM and Thiobacillus OTUs in

response to gypsum treatment indicating no tight linkage between these different bacteria (Fig.

3).

A smaller number of OTUs responded to gypsum in the bulk soil compartment, which reflects

the less pronounced separation of the respective microbial communities in the NMDS plot.

Among the 14 positive responders to gypsum, seven OTUs represented sulfate‐reducing taxa

within the Deltaproteobacteria (four Syntrophobacter OTUs, two unclassified

Syntrophobacteraceae OTUs, and one unclassified Desulfobulbaceae OTU) and one OTU

represented the sulfur‐oxidizing genus Thiobacillus. There was no clear trend towards a specific

metabolic group among the 13 negative responders; most belonged to the phyla Firmicutes and

Acidobacteria (Fig. 2). In contrast to the rhizosphere, abundance correlation networks of OTUs

responding positively to gypsum in bulk soil had a very low connectivity (≤4 edges, data not

shown) and were therefore not further analyzed. However, it was interesting to observe that

Desulfobulbaceae OTU 0085, which was among the top positive responders in the rhizosphere

(see above), was also one of the dominant positive responders in the bulk soil (Fig. 2,

Supporting Information Table S2). This OTU was remarkable in respect to its high relative

abundance compared to other OTUs, constituting 1.4 ± 0.3% in the rhizosphere and

0.14 ± 0.05% in the bulk soil under gypsum treatment. In comparison, other SRM‐affiliated

OTUs that responded positively to gypsum had a relative abundance of 0.08%–0.42% in the

rhizosphere and 0.06%–0.16% in the bulk soil (Supporting Information Table S2). Based on

this comparably high relative abundance and the central positioning in the rhizosphere response

network, a dominant role of Desulfobulbaceae OTU 0085 in sulfate reduction was indicated

under the applied conditions.

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Figure 3. Association network analysis visualizing strongly correlating abundance shifts

(r > 0.95, FDR‐corrected P‐value < 0.05) of OTUs responding positively to gypsum

amendment in the rhizosphere. The ID number of responding OTUs is displayed within or

besides representing circles. The strength of response towards gypsum is indicated by circle

size. Colours were chosen to give the best possible visual separation among different taxa (but

do not correspond to Fig. 2). Details on individual response OTUs can be found in Supporting

Information Table S2. To stabilize variance heterogeneity of OTU abundance data, regularized

log transformation was applied as implemented in the DESeq2 package (Love et al., 2014).

Resulting variance homogeneity was verified with Levene‘s test (P < 0.05). Transformed OTU

abundances of responsive OTUs were correlated pairwise to each other using Pearson

correlation. Significant (FDR‐corrected P‐value < 0.05) correlations with a correlation

coefficient r > 0.95 were used for network construction in the R package igraph 0.7.1 (Csárdi

and Nepusz, 2006). Plots were generated using the R package ggplot2 1.0.1 (Wickham, 2009).

In contrast to previous findings (Stubner, 2002; Scheid et al., 2004), Gram‐positive SRM

belonging to the genera Desulfosporosinus and Desulfotomaculum were not stimulated by

gypsum in our plant microcosms. Desulfotomaculum spp. were previously shown to be

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abundant in rice paddy soil, constituting 0.5% and 2% of the bacterial community in the bulk

soil and rhizosphere compartment, respectively (Stubner, 2002). However, not a single 16S

rRNA gene sequence in our dataset affiliated to this genus (data not shown), not even in the

freshly flooded soil that was dominated by members of the Firmicutes (Supporting Information

Fig. S4). Other members of the family Peptococcaceae were well detected by our approach

(data not shown) and the applied primers provide a good coverage of the genus

Desulfotomaculum (Quast et al., 2013). This hints either towards the absence of these SRM in

the soil used to initiate the experiment or a very low abundance that was below the detection

limit of our sequencing approach.

Root exudate‐based stable isotope probing

In the last 7–8 days of greenhouse incubations, two gypsum‐amended and two control

microcosms were incubated under an atmosphere that was labeled with 0.35% 13CO2 every half

hour during daytime. Two additional gypsum‐amended and two control microcosms served as

12C‐controls. One microcosm from each 13CO2‐treatment was analyzed for the isotopic

composition of emitted methane to determine the degree of 13C‐incorporation after 13CO2

assimilation by the plant and subsequent release of 13C‐labeled root exudates. Rapid labeling of

emitted methane after one day (Supporting Information Fig. S7) corroborated previous findings

(Lu and Conrad, 2005; Hernández et al., 2015) that rice plant photosynthesis is tightly coupled

to below‐ground organic carbon turnover including methanogenesis. Gypsum treatment had a

considerable effect on the degree of 13C‐incorporation into methane. While in the control

microcosm at least 10% of total methane produced was indicated to be plant‐derived carbon

over a period of eight days, it was only 4% in the gypsum‐amended microcosm (Supporting

Information Fig. S7). This indicated that microorganisms involved in sulfate reduction at least

partly competed with methanogenic archaea that are syntrophically linked to fermenters for

freshly produced plant‐derived carbon. However, RNA stable isotope probing of the bacterial

community in the rhizosphere revealed no differential labeling of microorganisms stimulated

by the gypsum treatment only (experimental procedure detailed in Supporting Information).

Microorganisms that were labeled both in the gypsum‐treated and the control rhizosphere

belonged to unclassified Burkholderiales and/or group‐3 Acidobacteria as well as unclassified

Geobacteraceae and/or subsection IV Cyanobacteria (Supporting Information Fig. S8). Most

likely, SRM were not detected against the background of aerobic microorganisms that are

affiliated to the micro‐oxic rhizosphere and are often labeled to a large extent in root‐exudate

labeling studies (Lu et al., 2006; Hernández et al., 2015).

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Conclusion

Sulfate amendment to rice paddy soil in the form of fertilizers or gypsum has been proposed as

one of several possible mitigation strategies to reduce methane emissions from rice paddy fields

(Schütz et al., 1989; Lindau et al., 1993; van der Gon and Neue, 1994; Yagi et al., 1997; van

der Gon et al., 2001). In addition, freshwater wetlands such as rice paddies are faced nowadays

with increased S deposition caused by extensive mining for fossil fuels and associated

combustion (Gauci et al., 2004) with an increasing trend in Asia, South America, and Africa

(Shah et al., 2000; Vallack et al., 2001). Here, we show that the application of gypsum to rice

plant microcosms largely preserved the bacterial community structure in the rhizosphere and

bulk soil while simultaneously reduced methane emissions by up to 99%. This effect coincided

with the stimulation of a small number of 66 OTUs in the rhizosphere and 14 OTUs in the bulk

soil, which were dominated in their response by sulfate reducing Deltaproteobacteria. Presence

of sulfate‐reducing Deltaproteobacteria in rice paddy soil has been previously established by

molecular snapshot analyses (Scheid and Stubner, 2001; Stubner, 2004; Lu et al., 2006; Knief

et al., 2011) and their potential activity by laboratory enrichment cultures (Wind et al., 1999).

Here, we provide evidence that these taxa (Desulfobulbaceae, Desulfobacteraceae,

Syntrophobacter, and Desulfovibrio) also respond in situ to increased sulfate availability. We

further identified an unclassified Rhodocyclaceae OTU and two Thiobacillus OTUs to be likely

part of the oxidative branch of sulfur cycling in rice paddy soil, which is besides Fe(II)

important in scavenging H2S produced by SRM that would otherwise function as a toxin to rice

plants (Lamers et al., 2013) and the associated microbial community.

Acknowledgement

We thank Melanie Klose for technical support and Bela Hausmann for advice using R. This

research was financed by the German Research Foundation (DFG, PE 2147/1‐1 to MP), the

European Union (FP7‐People‐2013‐CIG, Grant No PCIG14‐GA‐2013‐630188 to MP and

WETLANET, FP7 CSA‐support action, GA 229802 to NT), the Austrian Science Fund (FWF,

P23117‐B17 to MP and AL), the LOEWE Center for Synthetic Microbiology (RC) and the Max

Planck Society (JD).

Supplemental Material

The Supplementary Material for this article can be found online at:

https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1758-

2229.12413&file=emi412413-sup-0001-suppinfo1.pdf

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Chapter 3

The microbial succession of anaerobic chitin degradation in freshwater

sediments

Susanne Wörner and Michael Pester

Submitted in Applied and Environmental Microbiology (2019)

Abstract

Chitin is massively produced by freshwater plankton species as structural element of their

exoskeleton or cell wall. At the same time, chitin does not accumulate in the pre-dominantly

anoxic sediments, underlining its importance as carbon and nitrogen source for sedimentary

microorganisms. We studied chitin degradation in littoral sediment of Lake Constance as

Central Europe’s third largest lake. Turnover of the chitin analog methylumbelliferyl-N,N-

diacetylchitobioside (MUF-DC) was highest in the upper oxic sediment layer with 5.4 nmol

MUF-DC h-1 (g sediment dry weight)-1. In the underlying anoxic sediment layers, chitin

hydrolysis decreased with depth from 1.1 to 0.08 nmol MUF-DC h-1 (g sediment dry weight)-1.

Bacteria involved in chitin degradation were identified by 16S rRNA (gene) amplicon

sequencing of anoxic microcosms incubated in the presence of chitin as compared to

microcosms amended either with N-Acetylglucosamine as the monomer of chitin or no

substrate. Chitin degradation was driven by a succession of bacteria responding specifically to

chitin only. The early phase (0‒9 days) was dominated by Chitinivibrio spp. (Fibrobacteres).

The intermediate phase (9-21 days) was characterized by a higher diversity of chitin responders,

including besides Chitinivibrio spp. also members of the phyla Bacteroidetes, Proteobacteria,

Spirochaetes, and Chloroflexi. In the late phase (21-43 days), the Chitinivibrio populations

broke down at a parallel strong increase of Ruminiclostridium spp. (formerly Clostridium

cluster III, Firmicutes), which became the dominating chitin responders. Our study provides

quantitative insights into anaerobic chitin degradation in lake sediments and linked this to a

model of microbial succession associated with this activity.

Importance

Chitin is the most abundant biopolymer in aquatic environments with direct impact on the

carbon and nitrogen cycle. Despite its massive production as structural element of crustaceans,

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insects, or algae, it does not accumulate as detritus in sediments. However, little is known about

its turnover rate in pre-dominantly anoxic freshwater sediments and the microorganisms

involved in this activity. Here, we could prove that chitin is readily turned over under anoxic

conditions and link this to a succession of the responsible microbial community over a period

of 43 days. While bacteria affiliated to members of the Fibrobacteres and Firmicutes with

proven chitinolytic activity were driving the early and late phase of chitin degradation,

respectively, a more diverse community was involved in chitin degradation in the intermediate

phase. Our study provides quantitative insights into the microbial ecology driving anaerobic

chitin degradation in freshwater sediments.

Introduction

In freshwater environments, chitin is an important and abundant biopolymer with an estimated

annual production of 28 × 106 tons (Gooday, 1990; Cauchie, 2002). Chitin serves as structural

element of the exoskeleton of arthropods (e.g., crustaceans within the zooplankton and aquatic

insects), the cell wall of fungi, and certain algae and protozoa (Gooday, 1990). Its polymeric

structure consists of linked amino-sugar subunits with the disaccharide N,N-diacetylchitobiose

(GlcNAc)2 as the structural subunit and ß-1,4-N-acetyl-D-glucosamine (GlcNAc) as the

monomeric compound. Despite its high annual production, chitin does not accumulate in nature

(Beier and Bertilsson, 2013). This efficient turnover (50–75%) is also observed in freshwater

sediments (Beier and Bertilsson, 2013) and indicates chitin as an important carbon and nitrogen

source for microorganisms in these habitats (Gooday, 1990; Cauchie, 2002).

Cultured microorganisms that hydrolyze chitin and use it as a growth substrate under aerobic

conditions are found within the Beta-, Gamma- and Deltaproteobacteria, Firmicutes and

Actinobacteria, whereas most anaerobic chitinolytic microorganisms are found within the

Clostridia and Fibrobacteres (Evvyernie et al., 2000; Šimůnek et al., 2012; Brzezinska et al.,

2014; Garcia and Müller, 2014; Sorokin et al., 2014). However, chitinase genes have also been

found to be encoded by members of the Acidobacteria, Bacteroidetes, Cyanobacteria,

Spirochaetes and Chloroflexi (Gao et al., 2003; Karlsson and Stenlid, 2009). Chitin

depolymerization occurs first by cleavage of the polymer into water-soluble oligomers,

followed by splitting of these oligomers into dimers and finally the dimers into monomers. This

process involves endo- and exochitinases. Endochitinases randomly hydrolyze chitin, releasing

a mixture of oligomers of different length for further depolymerization. Exochitinases remove

mono- or disaccharides from the non-reducing end of chitin oligomers or chitin. In addition, β-

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N-acetylhexosaminidases (chitobiosidase) hydrolyze the dimer N,N-diacetylchitobiose

(Gooday, 1990; Dahiya et al., 2006; Karlsson and Stenlid, 2009).

Microbial polymer degradation is best understood for cellulose but the high structural similarity

of chitin and cellulose likely results in similar steps of the chitinolytic and cellulolytic pathways.

Under oxic conditions, bacteria secrete a mixture of soluble hydrolyzing enzymes that act

synergistically on the polymer (Wilson, 2008). Under anoxic conditions, microorganisms

(mainly Clostridia) were shown to be rather attached to the polymeric fiber and produce large

multienzyme complexes that are cell-associated outside the cell (Bayer et al., 2004). These

complexes are well-studied in cellulolytic microorganisms, but investigations of such

complexes are still missing in chitinolytic microorganisms (Stoykov et al., 2015; Bai et al.,

2016). The cell-bound enzyme complexes prevent cross-feeding of breakdown products and

enable at the same time a much higher turnover rate than a combination of different secreted

enzymes. Most of these hydrolytic microorganisms transport oligomers into the cell (Li and

Roseman, 2004), which are than cleaved to the monomeric structure and metabolized. This

cleavage is often mediated by phosphorylases rather than by hydrolytic cleavage, conserving

the energy in the linkage (Lou et al., 1997; Park et al., 2000; Kim et al., 2002; Zhang and Lynd,

2004). Another mechanism of anaerobic polymer turnover was observed in the phylum

Fibrobacteres, with all cultured members being specialized on anaerobic polymer degradation

(Suen et al., 2011; Ransom-Jones et al., 2012; Abdul Rahman et al., 2016). These bacteria also

attach to the polymeric substance and even lose their ability to hydrolyze the polymer if the

association is disturbed. Fibrobacteres members of the genera Chitinivibrio and

Chitinispirillum are specialists of chitin degradation, with Chitinivibrio spp. being even unable

to use polymeric chains shorter than (GlcNAc)n=6. All members transport polymer chains of

different length through the outer membrane into the periplasmatic space, where these

oligomers are cleaved and subsequently metabolized in the cell (Sorokin et al., 2014; Sorokin

et al., 2016) .

Environmental studies on chitin degradation focused mainly on soils and peatlands under

aerobic conditions. Here, members of the Beta- and Gammaproteobacteria, Acidobacteria and

Bacteroidetes were shown to be involved in chitin degradation (Kielak et al., 2013; Cretoiu et

al., 2014; Ivanova et al., 2016). The potential to degrade chitin under anaerobic conditions is

less well studied. In anoxic slurries of agricultural soil, which were investigated using chiA

(encoding chitinase A) as a functional marker gene, a large diversity of chiA with unknown

affiliation was detected but also chiA related to Beta- and Gammaproteobacteria (Wieczorek

et al., 2014). In contrast, a study focusing on anoxic wetland soil identified also members of the

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Firmicutes, Acidobacteria, Verrucomicrobia, and Alpha-, Beta-, Gamma-, and

Deltaproteobacteria to responded specifically to chitin amendment (Dai et al., 2016). In this

study, we investigated the microbial community responsible for the turnover of chitin and its

monomer GlcNAc under anoxic conditions in littoral sediment of oligotrophic Lake Constance.

We combined activity assays of chitin turnover with bacterial 16S rRNA gene and 16S rRNA

cDNA amplicon sequencing and could show that anaerobic chitin degradation occurs readily

and is mediated by a succession of different microbial taxa.

Results

Chitin is readily hydrolyzed in oxic and anoxic sediment layers

Chitinase activity could be measured in both, oxic and anoxic sediment layers, by following the

turnover of the chitin analog methylumbelliferyl-N,N-diacetylchitobioside (MUF-DC). Highest

chitinase activities were measured in the upper oxic sediment layer with 5.4 nmol MUF-DC h-1

and (g sediment dry weight)-1 (Figure 1). In anoxic sediment layers, chitin hydrolysis rates

showed a decreasing depth profile. Highest activity was measured 1 cm below surface with an

activity of 1.1 nmol MUF-DC h-1 and (g sediment dry weight)-1 and a decreasing hydrolysis

rate down to 5 cm below surface with a chitinase activity of 0.08 nmol MUF-DC h-1 and (g

sediment dry weight)-1.

Figure 1. Depth profile of chitinase activities in littoral sediment of Lake Constance. Chitin

hydrolysis rates were determined by measuring the turnover of the chitin analog

methylumbelliferyl-N,N-diacetylchitobioside (MUF-DC). Mean and one standard deviation are

shown for the respective sediment depths (n=3).

-5

-4

-3

-2

-1

0

1

0 2 4 6 8

Sed

imen

t d

epth

(cm

)

Chitinase activity

(nmol MUF-DC h-1 [g sediment dry weight] -1)

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In further experiments, we concentrated on chitin degradation under anoxic conditions. Anoxic

sediment microcosms were used to investigate the degradation of chitin and its monomer

GlcNAc. Release of ammonium, measured as an indicator for amino sugar degradation,

indicated a total chitin turnover of 2.4 mM (142 µmol) within the 43 days of incubation when

subtracting the amount of ammonium that accumulated in the no-substrate control (Figure 2,

Table 1). This translated into a turnover rate of 4.4 nmol chitin h-1 and (g sediment dry weight)-

1. Accumulation of GlcNAc due to chitin hydrolysis was not detected in chitin-amended

Figure 2. Time course of substrate amendment and product formation in chitin- or GlcNAc-

amended microcosms and their respective controls. The gap after the first seven days represents

the shift from pre-incubation to the single chitin or first GlcNAc amendment. Arrows indicate

days of sediment sampling. The mean and one standard deviation are shown, n=3. Some error

bars are smaller than the symbol size.

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microcosms. Among the tested substances butyrate, propionate, lactate, acetate, and formate,

chitin turnover resulted only in a small accumulation of acetate with a concentration of 97 ± 87

µM at the end of the incubation. CH4 accumulated in all microcosms, which was 3.6-fold higher

in the chitin-amended microcosms as compared to the respective control without external

substrate (Figure 2). An electron balance of the estimated chitin hydrolysis (32 e- per GlcNAc-

equivalent) revealed a recovery of 60% in CH4 (8 e- per molecule) and 1% in acetate (8 e- per

molecule) (Table 1).

Table 1. Overview of the sum of supplemented substrates and recovered products at the end of

the four different incubation setups. For each setup, the average and standard deviation of three

replicates are shown.

Substrates / Products (µmol)

Incubation line chitin Incubation line GlcNAc

+ chitin - chitin + GlcNAc - GlcNAc

Total amended amino-sugars 678 - 283 ± 11 -

Acetate 6 ± 5 n.d. 148 ± 16 n.d.

Propionate n.d. n.d. 6 ± 1 n.d.

Hydrogen n.d. n.d. n.d. n.d.

Methane 459 ± 42 128 ± 4 464 ± 22 166 ± 8

Total ammonium 167 ± 8 25 ± 2 229 ± 64 45 ± 6

n.d. – not detected

In GlcNAc-amended microcosms, periodically supplied GlcNAc was readily consumed

without apparent delay within three to four days. This translated in an average GlcNAc

degradation rate of 14.0 nmol GlcNAc h-1 and (g sediment dry weight)-1. Degradation resulted

in an accumulation of acetate to 2.7 ± 0.3 mM towards the end of the incubation; traces of

propionate with an end concentration of 100 ± 26 µM could be measured as well. Also in

GlcNAc-amended microcosms, CH4 was a major degradation product, which showed a 4.3-fold

higher accumulation in GlcNAc-amended microcosms as compared to the respective controls

without external substrate (Figure 2). Although ammonium accumulated steadily as well, only

65% could be recovered as would be expected from the total GlcNAc turnover (Table 1). An

electron balance of the degraded GlcNAc (32 e- per molecule) revealed a recovery of 26% in

CH4 (8 e-), 14% in acetate (8 e- per molecule), and 1% in propionate (14 e- per molecule) (Table

1).

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Chitin amendment altered a minor portion of bacterial beta diversity

At the onset of the microcosm experiment, the sediment harbored 14,262 ± 496 bacterial

species-level OTUs (mean ± st. dev.) when rarefied to an even sequencing depth of 68,826

reads per replicate (Figure S2) and 1.2 ± 0.4 × 108 16S rRNA gene copies (g sediment dry

weight)-1 (Figure S3). Both parameters stayed rather stable throughout the incubation period.

At the end of the individual incubation lines, measured alpha diversity was still at 12,797 ± 754

OTUs (Figure S2). Also total 16S rRNA gene copy numbers remained highly stable within 17

and 21 days for GlcNAc- and chitin-amended treatments, respectively. At the end of the

incubation time, GlcNAc-amended treatments revealed a slight increase (9.5 ± 2.8 × 108) and

chitin-amended treatments a slight decrease (6.2 ± 0.3 × 107) in 16S rRNA gene copy numbers

(Figure S3). Good´s coverage revealed that on average 91% of the bacterial alpha diversity was

covered with the following phyla representing each more than 1% relative abundance of the

detected 16S rRNA genes: Proteobacteria (classes Alpha-, Beta-, Gamma-, and

Deltaproteobacteria), Bacteroidetes, Chloroflexi, Actinobacteria, Acidobacteria,

Verrucomicrobia, Planctomycetes, Aminicenantes, Cyanobacteria/Chloroplast,

Ignavibacteriae, and unclassified bacteria. In the GlcNAc incubation line, Firmicutes and

Parcubacteria were in addition part of the highly abundant initial microbial community (Table

S1).

Temporal changes in beta diversity were analyzed using the weighted unifrac metric (Lozupone

et al., 2011) and visualized by a Principal Coordinate Analysis (PCoA). Starting communities

were highly similar within the individual incubation lines, both at the 16S rRNA and 16S rRNA

gene level. However, between the chitin and GlcNAc incubation line a slightly different

bacterial community was evident, which likely mirrored the different starting sediments (Figure

3). In all treatments, a slight segregation occurred after the onset of the experiment. Thereafter,

the bacterial communities in the chitin and GlcNAc controls (no substrate addition) showed no

further separation of the bacterial community over time, both on the 16S rRNA and 16S rRNA

gene level. On the contrary, substrate-amended microcosms showed a continuous separation

between all sampling days from the initial community at both the 16S rRNA gene and 16S

rRNA level, respectively. Differences between the chitin- and GlcNAc-amended microcosms

were more pronounced at the 16S rRNA than at the 16S rRNA gene level (Figure 3). The

observed shifts in community composition were significant (p-value < 0.05) in both substrate-

amended incubation lines as revealed by a PERMANOVA analysis. In chitin-amended

microcosms, 28% (31%) of the variation were explained by incubation time and 12% (10%) by

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chitin addition at the 16S rRNA (gene) level. Similar, in GlcNAc-amended microcosms, 26%

(28%) were explained by time and 19% (16%) by GlcNAc addition at the 16S rRNA (gene)

level.

Figure 3. Time-resolved beta-diversity of bacterial communities in the various microcosm

setups according to a principal coordinate analysis (PCoA) based on the weighted unifrac

metric. Segregation of the bacterial community over time is shown for the 16S rRNA gene and

16S rRNA amplicon survey separately. Connected points of the same color represent biological

replicates (n=3).

Chitin degradation triggers a succession of different responders

OTUs responsible for chitin-specific differences in beta-diversity were identified by significant

changes at the 16S rRNA or 16S rRNA gene level in response to chitin treatment over time. Of

the total bacterial community, 203 OTUs representing 13 phyla and unclassified bacteria

showed a significant response towards chitin amendment within the 43 days of incubation

(Table S2). In sum, responding OTUs steadily increased from 0.5 to 12.6% at the 16S rRNA

and from 0.5 to 8.0% at the 16S rRNA gene level throughout the incubation period. However,

there was a clear time-dependent succession of different response groups underlying this steady

increase, which was best evident at the 16S rRNA level (Figure 4). Nine days after chitin

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amendment, 30 OTUs affiliated to unclassified bacteria, Bacteroidetes, Spirochaetes,

Proteobacteria, Fibrobacteres, and Chloroflexi (decreasing number of OTUs) responded at the

16S rRNA level (Table S2). The two Chitinivibrio OTUs 318 and 1405 (Fibrobacteres, 1.4%

of all 16S rRNA copies) dominated this early response, followed by a pronounced decrease of

their 16S rRNA relative abundance towards the end of the incubation time (Figure 4). After 21

days of incubation, 68 additional OTUs responded at the 16S rRNA level. Besides the phyla

mentioned above, these intermediate responders represented the Acidobacteria,

Ignavibacteriae, Parcubacteria, Firmicutes and Cyanobacteria/Chloroplast (decreasing

number of OTUs). Transcriptionally most active intermediate responders were dominated by

unclassified bacteria and members of the phyla Bacteroidetes, Proteobacteria, Spirochaetes,

and Chloroflexi (in decreasing order) with none of the individual OTUs having distinctly more

Figure 4. Temporal changes of OTUs (summarized by phylum) at the 16S rRNA level that

significantly responded (FDR-corrected p-value < 0.05) to chitin over time and as compared to

the no-substrate control. Only phyla that corresponded in sum of their responding OTUs to

≥0.9% of bacterial 16S rRNA copies are shown for clarity. For details please refer to Table S2.

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16S rRNA copies than the others. The only phyla that harbored responders after 43 days only

were the Verrucomicrobia, Planctomycetes, and Candidate phylum BRC1 (Table S2).

However, in terms of relative 16S rRNA copy numbers, these late responders were clearly

dominated by the Clostridium cluster III OTU72 and OTU512 (Firmicutes), which together

represented 4.7% of all bacterial 16S rRNAs. With the exception of members of the order

Myxococcales (Deltaproteobacteria), which significantly responded at the 16S rRNA level

only, the described succession at the 16S rRNA level was largely mirrored at the 16S rRNA

gene level as well (Figure 4).

GlcNAc responders are clearly distinct from chitin responders

In the GlcNAc incubation line, 198 OTUs responded positively to regularly amended GlcNAc

over time and were represented by 13 phyla and unclassified bacteria (Table S3). During the

total incubation period of 27 days, responding OTUs increased in sum from 0.3 to 13.8% at the

16S rRNA and from 0.5 to 10.3% at the 16S rRNA gene level, respectively (Table S3). Again,

responses were strongest at the 16S rRNA level. However, in contrast to the chitin treatment,

the overall response was driven by members of just one phylum, the Proteobacteria (Figure 5).

Here, 85 response OTUs affiliated to the Proteobacteria accounted for 4.3%, 10.9% and 10.8%

of all 16S rRNA copy numbers after 7, 17 and 27 days, respectively (Table S3). Among these

OTUs, Uliginosibacterium OTU50 (Betaproteobacteria) clearly dominated the early response

after 7 days (2.5% of total 16S rRNA copy numbers). After 17 days, Azospirillum OTU85 and

unclassified OTU364 (both Alphaproteobacteria) as well as Rhodocyclaceae OTU66

(Betaproteobacteria) sharply increased in relative 16S rRNA copy numbers as well (1.1-2.1%

per OTU) but were still less abundant than Uliginosibacterium OTU50 (2.9%). Only after 27

days, Uliginosibacterium OTU50 declined (1.4%) and was outnumbered in relative 16S rRNA

copy numbers by Azospirillum OTU85 (3.3%) and Rhodocyclaceae OTU66 (2.3%).

Besides the Proteobacteria, 16 OTUs affiliated to Bacteroidetes, Fusobacteria, Firmicutes,

Spirochaetes, unclassified bacteria, and Cyanobacteria/Chloroplast (decreasing number of

OTUs) responded at the 16S rRNA level after 7 days of incubation as well (Table S3). Here,

members of the Fusobacteria (OTUs 139, 344 and 9344) constituted the second most dominant

response group after the Proteobacteria (1.6% of all 16S rRNA copies) but strongly declined

thereafter again (Figure 5). After 17 days of incubation, 35 additional OTUs responded at the

16S rRNA level and were affiliated, besides the phyla mentioned above, to the phyla

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Verrucomicrobia, Acidobacteria, Chloroflexi and Lentisphaerae (decreasing OTU numbers).

In terms of relative 16S rRNA copies, intermediate responders outside the Proteobacteria were

dominated by unclassified bacteria and members of the phyla Bacteroidetes, Fusobacteria and

Firmicutes (in decreasing order) with none of the individual OTUs being distinctly more

abundant than the others. Phyla that harbored responders after 27 days only were the phyla

Saccharibacteria and Parcubacteria and both were represented by just one OTU (Table S3).

However, in terms of relative 16S rRNA copy numbers, late responders outside the

Proteobacteria were dominated again by members of unclassified bacteria, Bacteroidetes, and

Firmicutes, which showed all an increasing response towards the end of the incubation time.

Figure 5. Temporal changes of OTUs (summarized by phylum) at the 16S rRNA level that

significantly responded (FDR-corrected p-value < 0.05) to GlcNAc over time and as compared

to the no-substrate control. Only phyla that corresponded in sum of their responding OTUs to

≥0.9% of bacterial 16S rRNA copies are shown for clarity. For details please refer to Table S3.

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Among the positively responding OTUs in chitin- (203) and GlcNAc- (198) amended

microcosms, only 23 OTUs responded under both treatments (7 Bacteroidetes, 2 Chloroflexi, 2

Firmicutes, 1 Betaproteobacterium, 1 Epsilonproteobacterium, 1 Cyanobacterium, 1

unclassified Proteobacterium, 1 unclassified Parcubacterium, and 7 unclassified bacteria)

(Table S4). None of these OTUs reached more than 0.27% (0.38%) of all 16S rRNA (gene)

copies at any time point of the respective treatments (Tables S2 and S3). In addition, members

of the phyla Spirochaetes, Acidobacteria and Planctomycetes that responded in either chitin-

or GlcNAc-amended treatments were represented by different OTUs within the respective

phyla.

Discussion

Anaerobic chitin turnover in incubations of littoral lake sediment could be readily observed

without any delay (Figure 2), which indicated the presence of indigenous microorganisms well

adapted to this substrate in Lake Constance. The observed rates were well comparable to chitin

turnover rates measured in aerobically incubated sediments and soils published previously

(Beier and Bertilsson, 2013). Depth-resolved chitinase activity, as measured by MUF-DC

hydrolyzing chitobiosidase, showed its highest activity in the upper oxic sediment layer and a

decreasing hydrolysis rate in anoxic sediment with depth. This depth dependence was observed

in other freshwater lakes as well (Köllner et al., 2012) and might reflect besides decreasing

chitin availability also different polymer turnover strategies in oxic as compared to anoxic

environments. Aerobic bacteria typically secrete soluble exo- and endochitinases in concert

with chitobiosidase to the environment that act synergistically on chitin hydrolysis to the

monomer GlcNAc, which is then taken up by the cell (Suzuki et al., 1998; Suzuki et al., 2002).

In contrast, most anaerobic polymer-hydrolyzing bacteria take up oligomeric structures into the

cell, where these are further depolymerized to mono- and disaccharides (Wilson, 2008).

Therefore, the employed enzymatic assay may have only captured extracellular chitobiosidase

activity outside the cell, leading to an underestimation of the actual anaerobic chitin hydrolysis

rate. This was reflected in the 4-fold higher chitin degradation rate in anoxic microcosms [4.4

nmol chitin h-1 and (g sediment dry weight)-1], which was estimated by the release of ammonia

from the amino-sugar, as compared to the chitobiosidase activity in fresh anoxic sediment

slurries.

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Chitin was efficiently mineralized to CH4 (and CO2, data not shown), as was also evident from

no transient accumulation of GlcNAc or possible fermentation intermediates with the exception

of a small amount of acetate (Figure 2). The electron balance indicated that about 60% of chitin

degradation could be attributed to the methanogenic degradation network, which typically

includes polymer hydrolysis, primary and secondary fermentations, and hydrogenotrophic

and/or acetoclastic methanogenesis (Schink and Stams, 2013). Microorganisms involved in

anaerobic respirations likely contributed to the remaining overall mineralization of chitin to

CO2. In Lake Constance sediments, activities of nitrate- (Hauck et al., 2001), Fe(III)- (Gerhardt

et al., 2005), humic acid- (Kappler et al., 2004) and sulfate-reducing microorganisms (Bak and

Pfennig, 1991a; b; Wörner and Pester, 2019) have been previously observed.

Chitin degradation in anoxic sediment microcosms could be linked to a continuous change of

bacterial beta diversity over time (Figure 3), which was attributed to a minor portion of the

overall community (see PERMANOVA results). This succession of the transcriptionally active

chitin-degrading bacterial community was also evident at the individual OTU level (Figure 4).

Three distinct phases could be identified. The early phase (0‒9 days) was clearly dominated by

two Chitinivibrio OTUs, which are members of the Fibrobacteres – a phylum that encompasses

polymer hydrolysis as a phylogenetically widespread trait (Abdul Rahman et al., 2016). The

only cultured representative of the genus Chitinivibrio (Chitinivibrio alkaliphilus) is an

obligately alkaliphilic bacterium isolated from an alkaline soda lake and strictly depends on the

polymer chitin as growth substrate, which it ferments to H2, acetate, glycerol, ethanol, and

formate (Sorokin et al., 2014). C. alkaliphilus grows by attaching to chitin and cells lyse

massively after chitin is degraded (Sorokin et al., 2014). This lifestyle conforms well to our

observations with both responding Chitinivibrio OTUs, which responded strongly in the early

phase followed by a complete breakdown of the population towards the end of the incubation.

In addition, our results show that metabolically active representatives of the genus Chitinivibrio

are not restricted to extreme environments like alkaline soda lakes but also play an important

role in chitin degradation in freshwater lakes.

The intermediate succession phase (9-21 days) of chitin degradation was characterized by a

higher diversity of dominant responders, which represented not only the Chitinivibrio OTUs of

the early phase but also unclassified bacteria and members of the phyla Bacteroidetes,

Proteobacteria, Spirochaetes, and Chloroflexi (Figure 4). The Bacteroidetes are known to

harbor polymer-hydrolyzing members, which are active under aerobic and anaerobic conditions

(Krieg et al., 2010). However, since the classification of responding Bacteroidetes OTUs was

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restricted to the phylum level little can be said whether the responding OTUs were directly

involved in chitin degradation or only indirectly by cross-feeding on the intermediate or

metabolic end products of the primary hydrolyzing bacteria. Proteobacteria, especially

members of the order Myxococcales, represented another dominant group of intermediate

responders. Since members of the Myxococcales (suborder Sorangiineae) are known to degrade

cellulose under aerobic conditions (Garcia et al., 2010), a primary involvement of the

responding Myxococcales in chitin hydrolysis and degradation seems feasible. The responding

members of the phylum Spirochaetes represent an interesting case. Although only one cultured

member of the Spirochaetes has so far been observed to hydrolyze polymeric structures in pure

culture (Aksenova et al., 1992), their association with hydrolyzing bacteria and the presence of

chitinolytic enzymes in their genomes indicate that these microorganisms have an important

impact during anaerobic polymer turnover (Pohlschroeder et al., 1994; Warnecke et al., 2007).

Supporting this notion, a recent study showed compelling evidence that members of the

spirochaetal genus Treponema are involved in hemicellulose degradation in the gut of wood-

feeding termites (Tokuda et al., 2018). Last but not least, most of the intermediate responders

that affiliated to the phylum Chloroflexi grouped within the family Anaerolineaceae, which has

only four cultured representatives that can grow anaerobically on different monosaccharides as

well as dimeric, oligomeric or polymeric structures (Yamada et al., 2006). However, our study

would be the first to indicate an involvement in anaerobic chitin degradation.

The late succession phase (21-43 days) of chitin degradation was characterized by a complete

breakdown of the responding Fibrobacteres and a strong increase of Firmicutes affiliated to the

Clostridium cluster III. While the latter became the dominating late responders, members of the

intermediate responders either increased further in their relative 16S rRNA (gene) copy

numbers or stayed relatively stable (Figure 4, Table S2). Clostridium cluster III was reclassified

as the genus Ruminiclostridium (Ludwig et al., 2009; Yutin and Galperin, 2013).

Ruminiclostridium spp. contain many polymer-hydrolyzing members that can use cellulose,

xylan or chitin as carbon and energy source (Leschine, 1995; Chin et al., 1998; Reguera and

Leschine, 2001). For example, Ruminiclostridium cellulolyticum, Ruminiclostridium

cellobioparum, and Ruminiclostridium hungatei have all proven chitinolytic activity under

anaerobic conditions (Reguera and Leschine, 2001; Zhang et al., 2018). As such, the responding

members of Clostridium cluster III (Ruminiclostridium spp.) were very likely directly involved

in chitin hydrolysis and turnover in the late phase of chitin degradation in our incubations.

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The specific microbial succession during anaerobic chitin degradation could be corroborated

by our control incubations using GlcNAc as the monomeric structure of chitin as substrate.

Here, OTUs that responded strongly under chitin amendment typically showed no significant

response in terms of their 16S rRNA (gene) copy numbers. This indicated that these

microorganisms clearly profit from chitin to a higher extent than from its monomer GlcNAc.

This was especially true for OTUs of the genera Chitinivibrio and Ruminiclostridium

(Clostridium cluster III), which are known to harbor chitinolytic species (Reguera and

Leschine, 2001; Sorokin et al., 2014) and were most responsive towards chitin, but showed no

response to the monomer GlcNAc. Vice versa, OTUs that strongly responded to the monomeric

compound GlcNAc, mainly Alpha- and Betaproteobacteria, showed no response towards

chitin. This also indicated once more that, if anything, only a small amount of the monomeric

compound GlcNAc is released into the environment during anaerobic chitin degradation. In

addition, no other intermediate degradation products accumulated to a large extent during the

incubation period. This reflects that the microorganisms involved in anaerobic chitin

degradations act very efficiently together resulting in a complete mineralization of this natural

and important polymer. In summary, our results provide comprehensive insights at which rate

chitin is degraded under anoxic conditions and link this to a model of microbial succession

associated with this activity.

Materials and Methods

Sediment and lake water sampling

Lake Constance is Central Europe’s third largest lake being situated in the pre-alpine region

between Germany, Austria, and Switzerland. It has currently an oligotrophic status and a warm-

monomictic mixing regime (Güde and Straile, 2016). Littoral sediment was sampled on

September 15, 2015, for the setup of microcosm experiments and on May 3, 2017, for chitinase

activity measurements. Sediment was taken from the littoral area of the Mainau Bay in Lake

Constance (N47° 42' 7'' E9° 11' 43''), Germany. All sediment cores were sampled using push

cores at 2 m water depth with plastic tubes of 80 mm inner diameter. The sediment structure

and layering could be preserved in the cores, spanning a depth of 0-30 cm and was covered with

the overlaying lake water.

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Chitin hydrolysis rates in lake sediment

Chitinase activity measurements were performed after the procedure described by Köllner et

al. (2012). In brief, sediment samples were taken in triplicates from the surface as well as at 1,

2, 3 and 5 cm below surface. Samples (0.5 g fresh weight) of each layer were immediately

transferred into 5-ml serum vials and flushed with N2 gas to provide anoxic conditions. Lake

water overlaying the sampled sediment cores was filter-sterilized with pore size 5 µm and 0.1

µm (Merck Millipore Ltd., Tullagreen, Ireland) by a peristaltic pump (Ismatec Ecoline

peristaltic pump, Cole-Parmer, Wertheim, Germany), autoclaved, made anoxic and treated with

formalin (Merck, Schuchardt, Hohenbrunn, Germany) to a final concentration of 0.25%.

Methylum-belliferyl-N,N´-diacetylchitobioside (MUF-DC, Sigma-Aldrich, St. Louis, US) was

dissolved in 100% dimethylformamide (DMF, Merck, Darmstadt, Germany). 1.5 ml of treated

lake water and 25 µM MUF-DC (end concentration) were amended under a constant flow of

100% N2 to the sediment in the 5-ml serum glass vials. Preparation of the slurries with surface

sediment were performed by the same procedure but under air. All slurries were incubated at

15°C for 23h in the dark without agitation. After incubation, slurries were mixed by shaking

and opened. 300 µl of the slurry were transferred to a 1.5-mL Eppendorf tube and mixed with

30 µl ammonium-glycine buffer (0.05 M glycine, 0.2 M ammonium hydroxide, adjusted to a

pH of 10.5 with NaOH) to stop chitinase activity. The mixture was incubated for 5 min at room

temperature to allow for sedimentation of particles. 200 µl of the supernatant were then

analyzed in a Varioskan Flash (Thermo Fisher Scientific, Waltham, USA) by applying an initial

shaking step (600 rpm) for 5 sec and thereafter a fluorescence measurement using an excitation

at 360 nm and measuring the emitted fluorescence at 460 nm. 1 g sediment fresh weight

corresponded to 0.62 g sediment dry weight as determined by drying the fresh sediment for two

weeks at 60°C.

Microcosm set up

Microcosms were setup on the same day as sediment sampling using the sediment layer of 1-3

cm below surface. For chitin and GlcNAc incubation lines, three sediment cores were sampled

each, homogenized under a constant stream of 100% N2, and divided into 6 150-ml glass bottles

by 50-g portions. Sediments were covered with 40 ml of anoxic, filter-sterilized (0.2 µm) lake

water that originated from the push core sampling and the headspace was flushed with 100%

N2 gas. Thereafter, microcosms were sampled for their initial sediment, sealed with butyl-

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rubber stoppers, and pre-incubated for seven days at 15°C to deplete internal substrates. After

pre-incubation, each incubation line was split into two separate sets of triplicates. For the chitin

incubation line, the first set of triplicates received once 150 mg purified chitin (setup “with

chitin”). The second set of triplicates served as control without external substrates (setup

“without chitin”). In the GlcNAc incubation line, the first set of triplicates received every 3-4

days a filter-sterilized (0.2 µm) GlcNAc solution (Fluka, Waltham, MA, USA) at a final

concentration of ca. 400 µM (setup “with GlcNAc”). The second set of triplicates served again

as control without external substrates (setup “without GlcNAc”) (Fig. S1).

To ensure that chitin was the only external substrate supplied to the chitin incubation setup,

commercially obtained chitin from crab shells (Sigma-Aldrich, Steinheim, Germany) was

purified using the following procedure before application: 5 g of crab shell chitin was dissolved

in 50 ml ice-cold HCl (25%) and stirred for 30 min. Thereafter, 250 ml of deionized, autoclaved

water was added and the suspension filtered through a 30 or 70 µm mesh (Franz Eckert GmbH,

Waldkirch, Germany). The chitin remaining on the filter was transferred to a glass vial and

washed once by the addition of 100 ml 50% ethanol (v/v). Thereafter, chitin was washed 4

times with 100 ml deionized, autoclaved water and filtered through a paper coffee filter. Again,

the chitin remaining on the coffee filter was transferred to a glass vial and washed again with

deionized, autoclaved water. Thereafter, the suspension was titrated towards neutral pH with 1

M NaOH. The suspension was filtered again through a paper coffee filter, chitin was removed

from the filter and lyophilized.

GlcNAc concentrations and degradation products of chitin and GlcNAc were monitored for

each microcosm every time the “with GlcNAc”-setup replicates were supplemented with

substrate. Gas samples from the headspace (200 µl) were monitored for accumulation of CH4

by gas chromatography (6000 Vega Series 2 GC, Carlo Erba, Italy), using a 45/60 carboxen

1000 column (Supleco, Oberhaching, Germany) operated at 120°C and a thermal conductivity

detector. 100% nitrogen gas was used as carrier gas at a column pressure of 60 kPa. The

injection port and detector were heated to 150°C and 180°C, respectively. Liquid samples (500

µl) were centrifuged (4°C, 14,000 × g, 5 min) and the supernatant was stored at -20°C until

analysis. Before analysis, liquid samples were centrifuged again (4°C, 14,000 × g, 5 min) and

the supernatant was used for measurements. Total ammonium (NH3 + NH4+) concentrations

were determined photometrically by a hypochlorite and phenol based method (Chaney and

Marbach, 1962). Formate, acetate, propionate, butyrate, lactate and GlcNAc were monitored by

HPLC (Shimadzu, Munich, Germany) equipped with an Aminex HPX87H column (BioRad,

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Munich, Germany) heated to 45°C with 10 mM H3PO4 as eluent at a flow rate of 1 ml min-1.

Analytes were detected with a photo diode array detector at 200 nm (Shimadzu). The detection

limit of all analyzed compounds was 10 µM.

Nucleic acid extraction and qPCR analysis

Sediment in the microcosms was sampled at four different time points. First samples were taken

right at the onset of the experiment as described above (initial sediment). The other samples

were collected after 9, 21 and 43 days of chitin amendment for chitin-amended and control

microcosms in the chitin incubation line. For the GlcNAc incubation line, samples were taken

after 7, 17 and 27 days after the first GlcNAc amendment, again both for substrate-amended

and control microcosms. Sediment samples were frozen immediately in liquid N2 and stored at

-60°C until further processing. RNA and DNA were extracted using the RNA PowerSoil® Total

RNA Isolation Kit in combination with the RNA PowerSoil® DNA elution Accessory Kit (Mo

Bio Laboratories Inc., Carlsbad, CA, USA). Remaining DNA in RNA extracts was removed

with the TURBO DNA-free Kit (Ambion, Thermo Fisher Scientific, Darmstadt, Germany).

Reverse transcription of RNA into cDNA was done using SuperScriptIII (life Technologies,

Darmstadt, Germany). Remaining RNA in DNA extracts was removed with RNase ONE

(Promega, Mannheim, Germany). Thereafter, RNA and DNA were quantified using Ribo- and

PicoGreen (Life Technologies), respectively. Quantitative PCR (qPCR) of total bacterial and

archaeal 16S rRNA genes was performed as described in (Wörner and Pester, 2019).

Amplicon sequencing and statistical analysis

Bacterial 16S rRNA genes and cDNA (V3-V4 region) were amplified using the universal

primer set 341F (CCT ACG GGN GGC WGC AG) and 802R (GAC TAC HVG GGT ATC

TAA TCC) as detailed in (Wörner and Pester, 2019). Sequence reads were quality controlled

and subjected to de novo chimera filtering (UCHIME, Edgar et al. (2011)) using Mothur v.

1.38.1 (Schloss, 2009). 19,421,489 high-quality reads remained and formed 408,146 species-

level OTUs (97% sequence identity). Each sample contained on average 16,581 ± 2,011 (mean

± st. dev.) OTUs. Taxonomic identity was assigned with the RDP Classifier (Wang et al., 2007)

and the RDP 16S rRNA training set 16 using a confidence threshold of 0.80.

Alpha diversity was compared among samples by rarefying all replicates to an even sequencing

depth in Mothur v. 1.38.1 (Schloss, 2009). All further analyses were performed without

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rarefaction using R, version 3.3.2 (R Core Team, 2016). To filter against remaining sequencing

artefacts and to enable meaningful statistical analysis, only OTUs detected by at least three

amplicon reads in at least three different replicates were analyzed. Differences in beta diversity

were analyzed using the weighted unifrac distance (Lozupone et al., 2011) in combination with

a principal coordinate analysis (PCoA) in the R phyloseq package 3.3.2 (McMurdie and

Holmes, 2013). In parallel, differences in beta diversity were tested for significance using a

permutational analysis of variance (PERMANOVA) of the weighted unifrac distances in the R

vegan package 2.5.1 (Oksanen et al., 2013). The package edgeR 3.16.5 (Robinson et al., 2010;

McCarthy et al., 2012) was used to test for significant (logFC ≥ 2, FDR-corrected p-value

<0.05) changes of relative OTU abundances according to both incubation time and incubation

line.

Sequence data availability

Amplicon data of 16S rRNA genes and 16S rRNA cDNA were deposited at the Sequence

Read Archive at NCBI (Leinonen et al., 2011) under the bioproject number PRJNA495895.

Acknowledgements

This project was funded by the Deutsche Forschungsgemeinschaft DFG (PE2147/1-1) and the

European Union (FP7-People-2013-CIG, Grant No PCIG14-GA-2013-630188). We thank

Evgenia Isaeva, Dominic Stickel, Sylke Wiechmann, and Alfred Sulger for technical support

and Bernhard Schink for providing lab space and inspiring discussions.

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Supplemental Material

Figure S1. Overview of the experimental set up. Time points of substrate amendment are shown

in green bars. Blue bars indicate sampling days for gas headspace (CH4 and H2) and liquid

sapmples (GlcNAc, short-chained fatty acids, total ammonium). Black bars indicate sampling

days for sediment slurries used for DNA and RNA extractions.

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Figure S2. Time-resolved changes in the number of observed bacterial species-level OTUs in

the individual treatments (16S rRNA gene analysis, 97% identity) when rarefied to an even

sequencing depth of 68,826 reads per replicate (n=3).

Figure S3. Abundance of total bacterial and archaeal 16S rRNA genes per gram sediment (dry

weight) in the various microcosm setups as revealed by qPCR. The mean and one standard

deviation are given, n = 3. Some error bars are smaller than the symbol size.

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Table S1. Overview of the total bacterial community at the phylum level (for Proteobacteria

class level) in the initial littoral sediment in the chitin (Table S1a) or GlcNAc (Table S1b)

incubation line.

16S rRNA genes (initial sediment)

Domain Phylum/Class (Proteobacteria) mean std

Bacteria unclassified 16.83 ± 0.95

Bacteria Cyanobacteria/Chloroplast 13.06 ± 1.00

Bacteria Chloroflexi 8.47 ± 0.19

Bacteria Gammaproteobacteria 8.31 ± 0.54

Bacteria Planctomycetes 7.77 ± 0.63

Bacteria Betaproteobacteria 7.46 ± 0.48

Bacteria Verrucomicrobia 7.25 ± 0.53

Bacteria Deltaproteobacteria 7.01 ± 0.50

Bacteria Bacteroidetes 6.98 ± 0.46

Bacteria Alphaproteobacteria 3.94 ± 0.36

Bacteria Acidobacteria 2.91 ± 0.25

Bacteria Actinobacteria 2.32 ± 0.45

Bacteria Ignavibacteriae 2.19 ± 0.17

Bacteria Aminicenantes 1.01 ± 0.10

Bacteria Parcubacteria 0.89 ± 0.10

Bacteria Firmicutes 0.81 ± 0.06

Bacteria Proteobacteria unclassified 0.54 ± 0.04

Bacteria Spirochaetes 0.48 ± 0.03

Bacteria Latescibacteria 0.35 ± 0.04

Bacteria candidate division ZB4 0.33 ± 0.04

Bacteria Gemmatimonadetes 0.16 ± 0.02

Bacteria Armatimonadetes 0.16 ± 0.01

Bacteria Hydrogenedentes 0.14 ± 0.02

Bacteria Candidatus Saccharibacteria 0.13 ± 0.01

Bacteria Deinococcus-Thermus 0.12 ± 0.01

Bacteria Lentisphaerae 0.09 ± 0.01

Bacteria Chlamydiae 0.08 ± 0.01

Bacteria Deferribacteres 0.07 ± 0.01

Bacteria BRC1 0.04 ± 0.01

Bacteria Cloacimonetes 0.04 ± 0.01

Bacteria SR2 0.03 ± 0.00

Bacteria Elusimicrobia 0.01 ± 0.00

unknown unclassified 0.01 ± 0.00

Bacteria Fusobacteria 0.01 ± 0.00

Bacteria Epsilonproteobacteria 0.004 ± 0.003

Bacteria Nitrospirae 0.004 ± 0.002

Archaea unclassified 0.003 ± 0.002

Bacteria Caldiserica 0.001 ± 0.002

Bacteria Microgenomates 0.001 ± 0.001

Bacteria Fibrobacteres 0.001 ± 0.001

Table S1a.

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16S rRNA genes (initial sediment)

Domain Phylum/Class (Proteobacteria) mean std

Bacteria unclassified 20.15 ± 1.02

Bacteria Chloroflexi 9.96 ± 0.44

Bacteria Cyanobacteria/Chloroplast 9.38 ± 0.62

Bacteria Gammaproteobacteria 8.16 ± 0.61

Bacteria Deltaproteobacteria 8.03 ± 0.56

Bacteria Bacteroidetes 7.55 ± 0.33

Bacteria Verrucomicrobia 6.97 ± 0.21

Bacteria Planctomycetes 6.59 ± 0.38

Bacteria Betaproteobacteria 6.40 ± 0.28

Bacteria Acidobacteria 2.84 ± 0.21

Bacteria Ignavibacteriae 2.77 ± 0.14

Bacteria Alphaproteobacteria 2.71 ± 0.23

Bacteria Actinobacteria 2.11 ± 0.32

Bacteria Aminicenantes 1.34 ± 0.18

Bacteria Parcubacteria 1.08 ± 0.15

Bacteria Firmicutes 1.05 ± 0.14

Bacteria Spirochaetes 0.58 ± 0.02

Bacteria Proteobacteria unclassified 0.46 ± 0.04

Bacteria Latescibacteria 0.43 ± 0.08

Bacteria candidate division WPS-2 0.19 ± 0.01

Bacteria Hydrogenedentes 0.17 ± 0.03

Bacteria Armatimonadetes 0.17 ± 0.02

Bacteria Lentisphaerae 0.14 ± 0.02

Bacteria Gemmatimonadetes 0.12 ± 0.02

Bacteria Chlamydiae 0.10 ± 0.02

Bacteria Candidatus Saccharibacteria 0.09 ± 0.01

Bacteria Deferribacteres 0.09 ± 0.02

Bacteria candidate division WPS-1 0.08 ± 0.01

Bacteria Deinococcus-Thermus 0.08 ± 0.01

Bacteria BRC1 0.06 ± 0.02

Bacteria Cloacimonetes 0.06 ± 0.01

Bacteria SR1 0.03 ± 0.00

Bacteria Elusimicrobia 0.02 ± 0.00

Bacteria Omnitrophica 0.02 ± 0.00

Bacteria Microgenomates 0.013 ± 0.001

Bacteria Epsilonproteobacteria 0.012 ± 0.003

Bacteria Nitrospirae 0.008 ± 0.001

Bacteria Fusobacteria 0.006 ± 0.002

Archaea unclassified 0.005 ± 0.003

unknown unclassified 0.004 ± 0.003

Bacteria candidate division ZB3 0.002 ± 0.001

Bacteria Fibrobacteres 0.001 ± 0.001

Table S1b.

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Table S2. Summary of all 16S rRNA (gene) OTUs at the approximate species-level (97% identity), which significantly responded (logFC>2, FDR-

corrected p-value <0.05) to chitin amendment as compared to the initial sediment and the respective timepoint in the no-substrate incubation. For each

OTU the phylogenetic affiliation, the logFC, the average relative abundance (n=3) in the individual treatments and timepoints at the 16S rRNA gene

and 16S rRNA level as well as the representative sequence is given.

LogFC value of significantly responding OTUs Mean relative abundance (%) - 16S rRNA genes Mean relative abundance (%) - 16S rRNA

16S rRNA genes 16S rRNA with chitin without chitin with chitin without chitin

OTU Phylum Class Order Family Genus 9 d 21 d

43 d 9 d

21 d

43 d

Initial sediment 9 d 21 d 43 d

Initial sediment 9 d 21 d 43 d

Initial sediment 9 d 21 d 43 d

Initial sediment 9 d 21 d 43 d

Otu0007996 Acidobacteria Acidobacteria_Gp17 Gp17 unclassified unclassified - - - - - 2.3 0.0003 0.0012 0.0015 0.0030 0.0007 0.0016 0.0004 0.0011 0.0004 0.0018 0.0029 0.0063 0.0006 0.0012 0.0009 0.0004

Otu0000628 Acidobacteria Holophagae Holophagales Holophagaceae Geothrix - 4.3 6.1 - 3.7 4.5 0.0015 0.0166 0.0487 0.1636 0.0025 0.0072 0.0088 0.0072 0.0043 0.0480 0.0705 0.1162 0.0031 0.0137 0.0102 0.0056

Otu0008421 Acidobacteria Holophagae Holophagales Holophagaceae Geothrix - - 3.3 - 2.1 2.4 0.0000 0.0003 0.0007 0.0095 0.0000 0.0000 0.0012 0.0004 0.0007 0.0019 0.0075 0.0079 0.0003 0.0020 0.0007 0.0000

Otu0002518 Acidobacteria Holophagae Holophagales Holophagaceae Holophaga - - 3.0 - - - 0.0010 0.0083 0.0113 0.0153 0.0010 0.0052 0.0059 0.0025 0.0056 0.0174 0.0235 0.0181 0.0022 0.0156 0.0087 0.0022

Otu0001732 Acidobacteria Holophagae Holophagales Holophagaceae unclassified - 3.2 3.4 - 4.6 3.4 0.0004 0.0038 0.0131 0.0144 0.0007 0.0006 0.0004 0.0004 0.0029 0.0258 0.0969 0.0389 0.0025 0.0083 0.0024 0.0020

Otu0002001 Acidobacteria Holophagae Holophagales Holophagaceae unclassified - - - - - 2.7 0.0021 0.0127 0.0199 0.0215 0.0013 0.0058 0.0053 0.0055 0.0038 0.0285 0.0172 0.0280 0.0028 0.0090 0.0117 0.0044

Otu0002350 Acidobacteria Holophagae Holophagales Holophagaceae unclassified - - 2.5 - 2.4 2.0 0.0013 0.0062 0.0109 0.0116 0.0009 0.0034 0.0023 0.0020 0.0049 0.0265 0.0313 0.0217 0.0018 0.0079 0.0042 0.0016

Otu0012233 Acidobacteria Holophagae Holophagales Holophagaceae unclassified - - 3.2 - - 3.2 0.0000 0.0000 0.0004 0.0091 0.0000 0.0006 0.0000 0.0004 0.0007 0.0000 0.0019 0.0162 0.0000 0.0003 0.0000 0.0000

Otu0005421 Bacteroidetes Sphingobacteriia Sphingobacteriales Sphingobacteriaceae unclassified - - - - 3.8 - 0.0006 0.0036 0.0030 0.0019 0.0000 0.0043 0.0011 0.0009 0.0000 0.0095 0.0164 0.0044 0.0000 0.0026 0.0023 0.0004

Otu0000121 Bacteroidetes unclassified unclassified unclassified unclassified - - - - - 2.9 0.0630 0.0749 0.1178 0.2749 0.0703 0.0925 0.0821 0.1018 0.0212 0.0385 0.0895 0.1515 0.0174 0.0283 0.0244 0.0244

Otu0000312 Bacteroidetes unclassified unclassified unclassified unclassified - - - - - 2.9 0.0449 0.0520 0.0633 0.1488 0.0333 0.0630 0.0454 0.0709 0.0089 0.0191 0.0388 0.0674 0.0113 0.0132 0.0145 0.0135

Otu0000641 Bacteroidetes unclassified unclassified unclassified unclassified - - - - - 2.0 0.0161 0.0211 0.0265 0.0566 0.0156 0.0223 0.0214 0.0260 0.0074 0.0129 0.0214 0.0305 0.0097 0.0125 0.0091 0.0072

Otu0000945 Bacteroidetes unclassified unclassified unclassified unclassified - - 5.4 - 2.6 3.9 0.0010 0.0009 0.0004 0.0899 0.0006 0.0019 0.0006 0.0053 0.0051 0.0005 0.0363 0.0853 0.0003 0.0006 0.0016 0.0004

Otu0001124 Bacteroidetes unclassified unclassified unclassified unclassified - - 3.7 - - - 0.0061 0.0033 0.0050 0.0860 0.0037 0.0064 0.0066 0.0068 0.0278 0.0466 0.0051 0.0420 0.0028 0.0281 0.0560 0.0274

Otu0001265 Bacteroidetes unclassified unclassified unclassified unclassified 4.3 5.3 - 4.6 6.2 2.9 0.0010 0.0405 0.0869 0.0062 0.0007 0.0067 0.0027 0.0000 0.0018 0.0717 0.2244 0.0193 0.0016 0.0099 0.0031 0.0000

Otu0001780 Bacteroidetes unclassified unclassified unclassified unclassified - - 4.2 - 2.7 4.4 0.0026 0.0034 0.0107 0.0613 0.0046 0.0049 0.0040 0.0044 0.0021 0.0046 0.0205 0.0654 0.0028 0.0030 0.0015 0.0040

Otu0001871 Bacteroidetes unclassified unclassified unclassified unclassified - - - - - 2.4 0.0031 0.0042 0.0093 0.0262 0.0036 0.0034 0.0063 0.0071 0.0107 0.0155 0.0314 0.0558 0.0059 0.0096 0.0090 0.0105

Otu0002159 Bacteroidetes unclassified unclassified unclassified unclassified 2.9 5.7 - 4.8 7.2 - 0.0000 0.0075 0.0581 0.0000 0.0000 0.0006 0.0000 0.0000 0.0000 0.0327 0.1781 0.0006 0.0006 0.0003 0.0007 0.0000

Otu0002465 Bacteroidetes unclassified unclassified unclassified unclassified - 3.4 5.0 3.2 4.8 5.6 0.0004 0.0041 0.0142 0.0443 0.0009 0.0006 0.0008 0.0008 0.0010 0.0205 0.0624 0.1031 0.0015 0.0029 0.0014 0.0028

Otu0002784 Bacteroidetes unclassified unclassified unclassified unclassified - - 3.1 - - - 0.0014 0.0011 0.0064 0.0190 0.0013 0.0040 0.0020 0.0038 0.0015 0.0052 0.0093 0.0117 0.0012 0.0026 0.0022 0.0057

Otu0003134 Bacteroidetes unclassified unclassified unclassified unclassified - - 4.0 - 3.4 3.8 0.0009 0.0027 0.0155 0.0326 0.0010 0.0028 0.0027 0.0028 0.0012 0.0061 0.0235 0.0285 0.0003 0.0044 0.0022 0.0020

Otu0003549 Bacteroidetes unclassified unclassified unclassified unclassified 3.3 4.3 - 3.5 4.9 - 0.0000 0.0098 0.0218 0.0004 0.0000 0.0018 0.0010 0.0016 0.0000 0.0125 0.0352 0.0000 0.0000 0.0020 0.0011 0.0023

Otu0004398 Bacteroidetes unclassified unclassified unclassified unclassified - 4.7 - 2.4 6.0 - 0.0000 0.0012 0.0279 0.0015 0.0000 0.0000 0.0000 0.0000 0.0007 0.0091 0.1254 0.0028 0.0003 0.0000 0.0000 0.0000

Otu0004412 Bacteroidetes unclassified unclassified unclassified unclassified - - 4.5 - - 5.1 0.0000 0.0003 0.0003 0.0238 0.0003 0.0009 0.0000 0.0004 0.0004 0.0004 0.0038 0.0497 0.0000 0.0009 0.0015 0.0017

Otu0004457 Bacteroidetes unclassified unclassified unclassified unclassified - - - - 3.2 4.0 0.0007 0.0000 0.0042 0.0048 0.0013 0.0003 0.0000 0.0004 0.0015 0.0037 0.0237 0.0394 0.0006 0.0009 0.0019 0.0006

Otu0005429 Bacteroidetes unclassified unclassified unclassified unclassified - - 3.1 - 3.2 5.3 0.0000 0.0009 0.0035 0.0082 0.0000 0.0000 0.0008 0.0000 0.0004 0.0000 0.0140 0.0560 0.0003 0.0003 0.0008 0.0009

Otu0005536 Bacteroidetes unclassified unclassified unclassified unclassified - - - - 3.5 - 0.0004 0.0031 0.0038 0.0000 0.0003 0.0033 0.0020 0.0000 0.0004 0.0093 0.0165 0.0000 0.0000 0.0088 0.0025 0.0000

Otu0006621 Bacteroidetes unclassified unclassified unclassified unclassified - 2.4 - 3.3 4.1 2.3 0.0004 0.0022 0.0070 0.0022 0.0000 0.0000 0.0000 0.0000 0.0003 0.0150 0.0260 0.0057 0.0003 0.0018 0.0004 0.0003

Otu0007366 Bacteroidetes unclassified unclassified unclassified unclassified - - - - - 2.0 0.0000 0.0003 0.0008 0.0013 0.0003 0.0000 0.0000 0.0000 0.0016 0.0015 0.0045 0.0093 0.0009 0.0009 0.0015 0.0007

Otu0008764 Bacteroidetes unclassified unclassified unclassified unclassified - - - - 2.7 3.6 0.0000 0.0003 0.0007 0.0056 0.0000 0.0000 0.0003 0.0000 0.0000 0.0043 0.0068 0.0131 0.0003 0.0003 0.0004 0.0000

Otu0008950 Bacteroidetes unclassified unclassified unclassified unclassified - - 2.9 - - 3.9 0.0000 0.0000 0.0007 0.0069 0.0011 0.0003 0.0000 0.0000 0.0004 0.0017 0.0066 0.0205 0.0003 0.0006 0.0004 0.0016

Otu0009674 Bacteroidetes unclassified unclassified unclassified unclassified - 2.8 - - 4.3 - 0.0000 0.0000 0.0070 0.0036 0.0000 0.0000 0.0000 0.0000 0.0000 0.0007 0.0228 0.0043 0.0000 0.0000 0.0000 0.0000

Otu0013428 Bacteroidetes unclassified unclassified unclassified unclassified - - - - 2.4 2.8 0.0000 0.0000 0.0006 0.0021 0.0003 0.0000 0.0000 0.0004 0.0008 0.0008 0.0093 0.0109 0.0003 0.0003 0.0015 0.0004

Otu0025199 Bacteroidetes unclassified unclassified unclassified unclassified - - - - 2.8 - 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0021 0.0074 0.0000 0.0000 0.0000 0.0000 0.0000

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Otu0007145 BRC1 unclassified unclassified unclassified unclassified - - 2.5 - - - 0.0008 0.0024 0.0027 0.0088 0.0018 0.0027 0.0036 0.0009 0.0011 0.0009 0.0011 0.0047 0.0018 0.0000 0.0010 0.0007

Otu0001022 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae Longilinea - - - - - 2.5 0.0040 0.0097 0.0048 0.0214 0.0064 0.0065 0.0073 0.0065 0.0117 0.0205 0.0302 0.0674 0.0092 0.0148 0.0153 0.0169

Otu0001945 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae Longilinea - 2.7 5.0 - 3.4 5.5 0.0011 0.0029 0.0128 0.0662 0.0010 0.0003 0.0000 0.0000 0.0033 0.0052 0.0471 0.1932 0.0006 0.0006 0.0011 0.0014

Otu0003087 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae Longilinea - - 2.6 - - 2.6 0.0022 0.0035 0.0083 0.0175 0.0022 0.0023 0.0012 0.0022 0.0037 0.0065 0.0165 0.0270 0.0009 0.0047 0.0023 0.0016

Otu0008257 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae Longilinea - - 3.2 - - 2.4 0.0003 0.0010 0.0018 0.0114 0.0003 0.0003 0.0000 0.0000 0.0011 0.0003 0.0004 0.0108 0.0000 0.0006 0.0000 0.0004

Otu0000209 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - - - - 2.7 0.1010 0.0868 0.1076 0.1452 0.1030 0.0864 0.0973 0.1170 0.0058 0.0074 0.0102 0.0422 0.0040 0.0100 0.0022 0.0009

Otu0000872 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - 5.3 5.2 - 5.9 4.6 0.0022 0.0091 0.1266 0.1152 0.0015 0.0031 0.0026 0.0024 0.0008 0.0060 0.1176 0.0445 0.0006 0.0009 0.0004 0.0003

Otu0001154 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - 4.3 7.1 - 5.0 7.3 0.0011 0.0027 0.0425 0.2937 0.0024 0.0006 0.0018 0.0014 0.0000 0.0041 0.0389 0.1752 0.0000 0.0003 0.0000 0.0010

Otu0001328 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - - 2.3 - - 0.0261 0.0180 0.0320 0.0165 0.0168 0.0139 0.0168 0.0228 0.0012 0.0101 0.0052 0.0008 0.0000 0.0017 0.0068 0.0015

Otu0001400 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - - - 2.3 - 0.0098 0.0083 0.0157 0.0389 0.0120 0.0171 0.0134 0.0185 0.0026 0.0047 0.0173 0.0260 0.0033 0.0058 0.0031 0.0070

Otu0001485 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - 2.9 7.0 - 3.9 7.4 0.0000 0.0009 0.0078 0.1409 0.0003 0.0015 0.0000 0.0004 0.0003 0.0010 0.0233 0.2437 0.0000 0.0006 0.0000 0.0003

Otu0002006 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - 5.1 6.9 - 4.4 5.3 0.0000 0.0011 0.0384 0.1355 0.0000 0.0012 0.0004 0.0008 0.0004 0.0016 0.0339 0.0585 0.0000 0.0000 0.0000 0.0004

Otu0002147 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - - - - 5.7 0.0012 0.0010 0.0030 0.0140 0.0024 0.0036 0.0019 0.0089 0.0004 0.0000 0.0264 0.0738 0.0003 0.0009 0.0004 0.0006

Otu0002537 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - 3.6 - - 4.2 0.0011 0.0017 0.0046 0.0243 0.0014 0.0013 0.0004 0.0000 0.0019 0.0035 0.0079 0.0503 0.0003 0.0026 0.0000 0.0006

Otu0003141 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - 3.8 - - 3.9 0.0027 0.0033 0.0092 0.0473 0.0019 0.0016 0.0014 0.0013 0.0007 0.0035 0.0090 0.0262 0.0009 0.0006 0.0011 0.0004

Otu0003178 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - 2.4 - - 2.4 0.0021 0.0029 0.0054 0.0150 0.0048 0.0040 0.0007 0.0028 0.0049 0.0046 0.0191 0.0286 0.0036 0.0047 0.0028 0.0029

Otu0003216 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - 4.2 - - - - 0.0011 0.0015 0.0389 0.0009 0.0100 0.0024 0.0012 0.0016 0.0000 0.0042 0.0041 0.0015 0.0000 0.0015 0.0021 0.0011

Otu0003489 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - 3.1 4.4 - 3.6 4.1 0.0010 0.0020 0.0171 0.0430 0.0003 0.0010 0.0004 0.0008 0.0003 0.0028 0.0185 0.0244 0.0003 0.0003 0.0000 0.0007

Otu0003605 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - - - - 3.3 0.0072 0.0003 0.0004 0.0000 0.0000 0.0007 0.0003 0.0000 0.0000 0.0027 0.0051 0.0099 0.0012 0.0014 0.0028 0.0013

Otu0004794 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - - - - 2.8 0.0007 0.0009 0.0016 0.0023 0.0018 0.0021 0.0026 0.0016 0.0015 0.0022 0.0082 0.0164 0.0009 0.0035 0.0032 0.0016

Otu0005012 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - 2.5 - 2.2 4.0 0.0003 0.0006 0.0018 0.0066 0.0000 0.0003 0.0000 0.0000 0.0015 0.0029 0.0112 0.0380 0.0009 0.0021 0.0019 0.0013

Otu0005357 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - 4.3 - - 3.0 0.0000 0.0006 0.0040 0.0204 0.0003 0.0025 0.0000 0.0029 0.0015 0.0015 0.0041 0.0187 0.0009 0.0012 0.0014 0.0003

Otu0005372 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - - - - 2.2 0.0023 0.0015 0.0038 0.0021 0.0022 0.0039 0.0008 0.0004 0.0000 0.0000 0.0019 0.0041 0.0000 0.0006 0.0000 0.0000

Otu0006615 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - - - - 2.5 0.0018 0.0007 0.0018 0.0067 0.0029 0.0025 0.0014 0.0021 0.0004 0.0026 0.0028 0.0068 0.0006 0.0018 0.0018 0.0000

Otu0008128 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - 3.9 - - 3.7 0.0003 0.0000 0.0007 0.0195 0.0000 0.0000 0.0000 0.0000 0.0012 0.0000 0.0030 0.0278 0.0000 0.0000 0.0000 0.0000

Otu0008871 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - - - - 2.8 0.0021 0.0000 0.0003 0.0035 0.0009 0.0000 0.0007 0.0000 0.0007 0.0000 0.0012 0.0111 0.0003 0.0000 0.0004 0.0000

Otu0010112 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - - - - 2.6 0.0000 0.0009 0.0035 0.0122 0.0007 0.0009 0.0004 0.0031 0.0000 0.0012 0.0042 0.0060 0.0003 0.0006 0.0003 0.0007

Otu0011635 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - - - - 2.6 0.0006 0.0003 0.0015 0.0012 0.0000 0.0009 0.0007 0.0004 0.0000 0.0000 0.0016 0.0056 0.0003 0.0003 0.0011 0.0003

Otu0000094 Cyanobacteria/Chloroplast Chloroplast Chloroplast Bacillariophyta unclassified 4.0 - 4.5 - - - 0.0102 0.1753 0.1789 0.2468 0.0174 0.0086 0.4757 0.0336 0.0057 0.0053 0.0004 0.0031 0.0019 0.0008 0.0049 0.0076

Otu0000433 Cyanobacteria/Chloroplast Cyanobacteria Family_II GpIIa unclassified - 3.3 - - - - 0.0052 0.0235 0.0571 0.0449 0.0242 0.0028 0.0018 0.0410 0.1382 0.0591 0.2331 0.0072 0.0802 0.0727 0.1356 0.0050

Otu0000036 Cyanobacteria/Chloroplast Cyanobacteria unclassified unclassified unclassified - - - - 4.9 - 0.0423 0.0036 0.1041 0.0617 0.0477 0.0044 0.1089 0.0897 0.0162 0.0102 0.5320 0.0144 0.3034 0.0112 0.0204 0.2059

Otu0001299 Cyanobacteria/Chloroplast Cyanobacteria unclassified unclassified unclassified 2.3 - - - - - 0.0044 0.0273 0.0073 0.0151 0.0006 0.0000 0.0011 0.0254 0.0333 0.0012 0.0484 0.0003 0.0304 0.0484 0.0298 0.0199

Otu0000318 Fibrobacteres Chitinivibrionia Chitinivibrionales Chitinivibrionaceae Chitinivibrio 6.9 7.1 4.4 8.9 8.0 3.6 0.0003 0.1667 0.1993 0.0293 0.0000 0.0000 0.0000 0.0000 0.0011 1.0952 0.6214 0.0259 0.0000 0.0017 0.0003 0.0000

Otu0001405 Fibrobacteres Chitinivibrionia Chitinivibrionales Chitinivibrionaceae Chitinivibrio 5.8 5.5 4.2 7.5 7.0 5.3 0.0000 0.0624 0.0506 0.0189 0.0000 0.0000 0.0000 0.0000 0.0004 0.2682 0.2039 0.0561 0.0000 0.0003 0.0000 0.0003

Otu0002297 Fibrobacteres Chitinivibrionia Chitinivibrionales Chitinivibrionaceae Chitinivibrio - 6.6 4.4 - 6.1 - 0.0000 0.0020 0.1117 0.0228 0.0000 0.0000 0.0000 0.0000 0.0000 0.0045 0.0863 0.0167 0.0000 0.0000 0.0000 0.0000

Otu0005991 Fibrobacteres Fibrobacteria Fibrobacterales Fibrobacteraceae Fibrobacter - 4.2 - - 4.7 - 0.0000 0.0019 0.0209 0.0011 0.0000 0.0000 0.0000 0.0000 0.0008 0.0061 0.0507 0.0029 0.0000 0.0000 0.0000 0.0000

Otu0002337 Firmicutes Clostridia Clostridiales Natranaerovirga unclassified - - 7.3 - - 7.0 0.0000 0.0000 0.0004 0.1786 0.0000 0.0000 0.0000 0.0000 0.0007 0.0000 0.0000 0.2293 0.0000 0.0000 0.0000 0.0003

Otu0000072 Firmicutes Clostridia Clostridiales Ruminococcaceae Clostridium_III - - 9.9 - 5.2 ## 0.0000 0.0005 0.0155 1.1103 0.0000 0.0000 0.0000 0.0000 0.0017 0.0009 0.1078 3.2070 0.0000 0.0000 0.0000 0.0027

Otu0000512 Firmicutes Clostridia Clostridiales Ruminococcaceae Clostridium_III - - 8.2 - 3.4 9.2 0.0000 0.0000 0.0049 0.3193 0.0000 0.0003 0.0000 0.0000 0.0014 0.0015 0.0275 1.5093 0.0000 0.0000 0.0004 0.0000

Otu0007275 Firmicutes Clostridia Clostridiales Ruminococcaceae Clostridium_III - - 4.1 - - 5.3 0.0000 0.0000 0.0010 0.0171 0.0000 0.0000 0.0000 0.0000 0.0000 0.0006 0.0058 0.0455 0.0000 0.0000 0.0000 0.0003

Otu0009375 Firmicutes Clostridia Clostridiales Ruminococcaceae Saccharofermentans - - 2.6 - - - 0.0017 0.0009 0.0026 0.0153 0.0009 0.0025 0.0022 0.0017 0.0000 0.0000 0.0000 0.0037 0.0000 0.0000 0.0000 0.0003

Otu0027005 Firmicutes Clostridia Clostridiales Ruminococcaceae unclassified - - - - - 2.2 0.0000 0.0000 0.0000 0.0008 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0043 0.0000 0.0000 0.0000 0.0000

Otu0015348 Firmicutes Clostridia Clostridiales Syntrophomonadaceae unclassified - - 2.3 - - - 0.0004 0.0013 0.0039 0.0059 0.0000 0.0000 0.0007 0.0000 0.0007 0.0008 0.0004 0.0011 0.0003 0.0000 0.0007 0.0000

Otu0003707 Firmicutes Clostridia Clostridiales unclassified unclassified - 4.0 - - - - 0.0004 0.0118 0.0223 0.0235 0.0003 0.0078 0.0041 0.0072 0.0000 0.0054 0.0041 0.0067 0.0003 0.0029 0.0010 0.0020

Otu0004472 Firmicutes Clostridia Clostridiales unclassified unclassified - - 2.2 - - - 0.0032 0.0040 0.0055 0.0169 0.0038 0.0037 0.0036 0.0018 0.0000 0.0007 0.0004 0.0042 0.0003 0.0003 0.0004 0.0000

Otu0013817 Firmicutes Clostridia Clostridiales unclassified unclassified - - 3.9 - - - 0.0003 0.0003 0.0004 0.0189 0.0000 0.0003 0.0000 0.0004 0.0000 0.0000 0.0007 0.0032 0.0000 0.0000 0.0000 0.0000

Otu0004716 Firmicutes Negativicutes Selenomonadales Veillonellaceae unclassified - 2.5 3.9 - - 2.0 0.0003 0.0018 0.0072 0.0196 0.0000 0.0000 0.0000 0.0007 0.0004 0.0000 0.0008 0.0053 0.0000 0.0006 0.0000 0.0003

Otu0008929 Firmicutes Negativicutes Selenomonadales Veillonellaceae unclassified - - 3.6 - - - 0.0007 0.0019 0.0041 0.0201 0.0007 0.0000 0.0013 0.0004 0.0000 0.0006 0.0004 0.0016 0.0003 0.0000 0.0000 0.0000

Otu0014118 Firmicutes Negativicutes Selenomonadales Veillonellaceae unclassified - - 2.9 - - 2.8 0.0000 0.0003 0.0019 0.0070 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0008 0.0017 0.0000 0.0000 0.0000 0.0003

Otu0001327 Ignavibacteriae Ignavibacteria Ignavibacteriales Ignavibacteriaceae Ignavibacterium - - 5.2 - 3.6 6.1 0.0015 0.0017 0.0074 0.0867 0.0012 0.0012 0.0004 0.0004 0.0050 0.0040 0.0728 0.3862 0.0012 0.0009 0.0047 0.0066

Otu0001421 Ignavibacteriae Ignavibacteria Ignavibacteriales Ignavibacteriaceae Ignavibacterium - - 5.1 - 3.9 5.9 0.0013 0.0038 0.0089 0.0807 0.0003 0.0034 0.0042 0.0004 0.0026 0.0083 0.0570 0.2073 0.0006 0.0035 0.0022 0.0038

Otu0005927 Ignavibacteriae Ignavibacteria Ignavibacteriales Ignavibacteriaceae Ignavibacterium - - - - 3.0 - 0.0003 0.0003 0.0003 0.0025 0.0007 0.0000 0.0004 0.0019 0.0003 0.0048 0.0116 0.0132 0.0026 0.0014 0.0000 0.0047

Otu0007864 Ignavibacteriae Ignavibacteria Ignavibacteriales Ignavibacteriaceae Ignavibacterium - - - - - 2.7 0.0012 0.0003 0.0000 0.0026 0.0000 0.0000 0.0000 0.0004 0.0011 0.0034 0.0081 0.0126 0.0009 0.0023 0.0011 0.0019

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Otu0001781 Parcubacteria unclassified unclassified unclassified unclassified - 2.7 4.0 - 4.4 3.4 0.0056 0.0022 0.0431 0.1049 0.0060 0.0052 0.0016 0.0008 0.0022 0.0012 0.0696 0.0327 0.0022 0.0006 0.0010 0.0013

Otu0002202 Parcubacteria unclassified unclassified unclassified unclassified - 6.7 7.0 - 4.8 4.0 0.0003 0.0085 0.1507 0.1770 0.0003 0.0018 0.0008 0.0000 0.0000 0.0056 0.0321 0.0179 0.0000 0.0000 0.0000 0.0000

Otu0004551 Parcubacteria unclassified unclassified unclassified unclassified - - 3.2 - - - 0.0024 0.0007 0.0031 0.0299 0.0016 0.0026 0.0014 0.0038 0.0000 0.0003 0.0004 0.0038 0.0000 0.0003 0.0000 0.0003

Otu0005795 Parcubacteria unclassified unclassified unclassified unclassified - 4.4 5.4 - 4.0 4.2 0.0000 0.0011 0.0227 0.0479 0.0000 0.0000 0.0000 0.0000 0.0003 0.0010 0.0238 0.0268 0.0000 0.0003 0.0000 0.0000

Otu0008698 Parcubacteria unclassified unclassified unclassified unclassified - - 2.9 - - - 0.0000 0.0008 0.0048 0.0068 0.0000 0.0006 0.0000 0.0000 0.0000 0.0000 0.0014 0.0004 0.0000 0.0000 0.0000 0.0000

Otu0003283 Planctomycetes Planctomycetia Planctomycetales Planctomycetaceae unclassified - - 2.0 - - - 0.0012 0.0021 0.0041 0.0080 0.0054 0.0063 0.0031 0.0015 0.0030 0.0025 0.0049 0.0044 0.0003 0.0012 0.0015 0.0031

Otu0000498 Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae Chitinilyticum - 3.0 3.2 - - 2.8 0.0000 0.0036 0.0082 0.0094 0.0000 0.0025 0.0004 0.0000 0.0003 0.0022 0.0071 0.0091 0.0006 0.0017 0.0007 0.0000

Otu0003387 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae unclassified - - - - 3.2 - 0.0014 0.0324 0.0356 0.0215 0.0016 0.0055 0.0092 0.0193 0.0016 0.0221 0.0244 0.0074 0.0006 0.0028 0.0048 0.0038

Otu0004577 Proteobacteria Deltaproteobacteria Desulfuromonadales Geobacteraceae Geobacter - - - - - 5.5 0.0000 0.0003 0.0061 0.0183 0.0000 0.0000 0.0006 0.0043 0.0000 0.0003 0.0145 0.0525 0.0000 0.0000 0.0031 0.0102

Otu0008472 Proteobacteria Deltaproteobacteria Desulfuromonadales Geobacteraceae Geobacter - - - - - 3.3 0.0000 0.0000 0.0000 0.0035 0.0000 0.0000 0.0000 0.0005 0.0000 0.0000 0.0007 0.0103 0.0000 0.0003 0.0007 0.0007

Otu0009817 Proteobacteria Deltaproteobacteria Myxococcales Kofleriaceae Kofleria - - - - - 2.4 0.0000 0.0003 0.0000 0.0009 0.0000 0.0000 0.0007 0.0011 0.0004 0.0003 0.0034 0.0066 0.0000 0.0009 0.0013 0.0007

Otu0002097 Proteobacteria Deltaproteobacteria Myxococcales Phaselicystidaceae Phaselicystis - 2.9 - 3.8 6.4 5.5 0.0004 0.0019 0.0104 0.0078 0.0000 0.0000 0.0000 0.0000 0.0011 0.0300 0.1929 0.0947 0.0024 0.0017 0.0015 0.0000

Otu0002423 Proteobacteria Deltaproteobacteria Myxococcales Phaselicystidaceae Phaselicystis - - - - - 2.1 0.0027 0.0026 0.0035 0.0038 0.0016 0.0010 0.0000 0.0020 0.0071 0.0240 0.0319 0.0320 0.0072 0.0111 0.0088 0.0047

Otu0002817 Proteobacteria Deltaproteobacteria Myxococcales Phaselicystidaceae Phaselicystis - - 3.9 - 2.2 3.5 0.0012 0.0021 0.0037 0.0314 0.0009 0.0000 0.0000 0.0000 0.0086 0.0159 0.0448 0.1062 0.0102 0.0006 0.0000 0.0000

Otu0003609 Proteobacteria Deltaproteobacteria Myxococcales Phaselicystidaceae Phaselicystis - - - 4.3 4.8 6.3 0.0000 0.0000 0.0014 0.0035 0.0003 0.0000 0.0000 0.0000 0.0000 0.0220 0.0334 0.0909 0.0021 0.0038 0.0033 0.0022

Otu0009301 Proteobacteria Deltaproteobacteria Myxococcales Phaselicystidaceae Phaselicystis - - - 2.5 4.3 2.8 0.0000 0.0003 0.0004 0.0017 0.0000 0.0000 0.0000 0.0000 0.0003 0.0074 0.0305 0.0092 0.0018 0.0003 0.0000 0.0000

Otu0016167 Proteobacteria Deltaproteobacteria Myxococcales Phaselicystidaceae Phaselicystis - - - - - 2.7 0.0004 0.0000 0.0000 0.0018 0.0000 0.0003 0.0000 0.0000 0.0011 0.0010 0.0019 0.0127 0.0003 0.0012 0.0000 0.0010

Otu0002828 Proteobacteria Deltaproteobacteria Myxococcales Polyangiaceae unclassified - - 3.3 - 6.4 6.2 0.0004 0.0003 0.0086 0.0133 0.0000 0.0000 0.0000 0.0000 0.0007 0.0000 0.1646 0.1335 0.0000 0.0000 0.0000 0.0000

Otu0002051 Proteobacteria Deltaproteobacteria Myxococcales unclassified unclassified - - - - 2.7 6.3 0.0000 0.0000 0.0000 0.0047 0.0000 0.0000 0.0000 0.0000 0.0011 0.0011 0.0141 0.1667 0.0000 0.0003 0.0004 0.0006

Otu0004900 Proteobacteria Deltaproteobacteria Myxococcales unclassified unclassified - - - 2.9 4.5 2.8 0.0008 0.0016 0.0054 0.0060 0.0003 0.0003 0.0000 0.0000 0.0010 0.0155 0.0503 0.0142 0.0025 0.0023 0.0014 0.0000

Otu0005849 Proteobacteria Deltaproteobacteria Myxococcales unclassified unclassified - - - 2.6 4.3 4.5 0.0000 0.0006 0.0014 0.0035 0.0003 0.0000 0.0000 0.0000 0.0003 0.0085 0.0313 0.0333 0.0006 0.0009 0.0003 0.0000

Otu0011616 Proteobacteria Deltaproteobacteria Myxococcales unclassified unclassified - - 2.7 - - 4.9 0.0004 0.0003 0.0004 0.0080 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0023 0.0338 0.0000 0.0000 0.0000 0.0000

Otu0019478 Proteobacteria Deltaproteobacteria Myxococcales unclassified unclassified - - - - - 2.8 0.0000 0.0000 0.0000 0.0009 0.0000 0.0000 0.0000 0.0000 0.0003 0.0002 0.0000 0.0093 0.0000 0.0000 0.0000 0.0000

Otu0008641 Proteobacteria Deltaproteobacteria Syntrophorhabdaceae Syntrophorhabdus unclassified - - - - - 2.2 0.0004 0.0005 0.0007 0.0015 0.0000 0.0006 0.0015 0.0004 0.0007 0.0028 0.0019 0.0075 0.0009 0.0023 0.0010 0.0010

Otu0002177 Proteobacteria Deltaproteobacteria unclassified unclassified unclassified - 6.3 5.5 - 5.5 4.5 0.0000 0.0000 0.0928 0.0481 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0536 0.0251 0.0000 0.0000 0.0000 0.0000

Otu0004082 Proteobacteria Deltaproteobacteria unclassified unclassified unclassified - 5.3 4.7 - 4.3 3.5 0.0003 0.0005 0.0568 0.0348 0.0010 0.0009 0.0003 0.0009 0.0000 0.0000 0.0226 0.0120 0.0000 0.0003 0.0000 0.0000

Otu0010548 Proteobacteria Deltaproteobacteria unclassified unclassified unclassified - 2.4 - - - - 0.0011 0.0016 0.0103 0.0154 0.0007 0.0003 0.0000 0.0004 0.0000 0.0000 0.0042 0.0037 0.0000 0.0000 0.0000 0.0003

Otu0000567 Proteobacteria Epsilonproteobacteria Campylobacterales Campylobacteraceae Sulfurospirillum - - 4.1 - - - 0.0000 0.0014 0.0075 0.0179 0.0000 0.0000 0.0003 0.0024 0.0000 0.0007 0.0065 0.0064 0.0000 0.0000 0.0004 0.0368

Otu0000447 Proteobacteria Gammaproteobacteria Methylococcales Methylococcaceae Methylobacter 4.5 - - - - - 0.0048 0.1333 0.1148 0.0734 0.0721 0.0031 0.0505 0.0760 0.0060 0.0075 0.0076 0.0061 0.0039 0.0068 0.0088 0.0089

Otu0001893 Proteobacteria unclassified unclassified unclassified unclassified - 6.3 4.0 - 6.5 - 0.0000 0.0000 0.0865 0.0170 0.0000 0.0000 0.0000 0.0000 0.0004 0.0004 0.1431 0.0021 0.0000 0.0000 0.0000 0.0000

Otu0008418 Proteobacteria unclassified unclassified unclassified unclassified - - - - - 2.8 0.0004 0.0005 0.0004 0.0038 0.0000 0.0009 0.0004 0.0008 0.0004 0.0013 0.0047 0.0094 0.0018 0.0024 0.0007 0.0014

Otu0009443 Proteobacteria unclassified unclassified unclassified unclassified - - - - - 3.6 0.0004 0.0000 0.0007 0.0029 0.0003 0.0000 0.0000 0.0000 0.0007 0.0000 0.0038 0.0207 0.0006 0.0000 0.0007 0.0000

Otu0000393 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae Spirochaeta 5.9 7.6 7.1 6.3 7.1 6.9 0.0004 0.0892 0.2973 0.2017 0.0000 0.0013 0.0000 0.0000 0.0004 0.1224 0.2206 0.1798 0.0003 0.0000 0.0000 0.0004

Otu0001208 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae Spirochaeta - 6.2 7.9 - 6.6 8.3 0.0000 0.0020 0.0861 0.2694 0.0000 0.0000 0.0000 0.0000 0.0000 0.0061 0.1206 0.3531 0.0003 0.0000 0.0000 0.0000

Otu0001809 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae Spirochaeta - 5.1 3.3 2.9 5.2 3.6 0.0000 0.0055 0.0387 0.0090 0.0000 0.0000 0.0000 0.0000 0.0000 0.0083 0.0437 0.0131 0.0000 0.0003 0.0003 0.0000

Otu0004046 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae Spirochaeta - - - 3.5 - - 0.0000 0.0192 0.0083 0.0021 0.0000 0.0000 0.0000 0.0000 0.0000 0.0124 0.0063 0.0003 0.0000 0.0003 0.0000 0.0000

Otu0005835 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae Spirochaeta - - 5.5 - - 4.3 0.0000 0.0000 0.0262 0.0468 0.0000 0.0000 0.0010 0.0000 0.0007 0.0009 0.0134 0.0348 0.0000 0.0003 0.0000 0.0000

Otu0009381 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae Spirochaeta - 3.5 2.5 - 2.3 - 0.0000 0.0032 0.0117 0.0049 0.0000 0.0000 0.0000 0.0000 0.0000 0.0039 0.0049 0.0018 0.0000 0.0000 0.0000 0.0000

Otu0002565 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae Treponema - 3.6 5.8 2.5 3.7 5.2 0.0000 0.0009 0.0128 0.0610 0.0000 0.0003 0.0007 0.0005 0.0007 0.0101 0.0241 0.0640 0.0010 0.0012 0.0010 0.0006

Otu0010694 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae Treponema - - - - - 4.2 0.0000 0.0003 0.0015 0.0033 0.0000 0.0000 0.0000 0.0004 0.0000 0.0006 0.0046 0.0209 0.0006 0.0000 0.0011 0.0000

Otu0012123 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae Treponema - - - - - 2.6 0.0000 0.0003 0.0014 0.0025 0.0003 0.0003 0.0004 0.0000 0.0000 0.0008 0.0043 0.0061 0.0000 0.0003 0.0000 0.0000

Otu0000818 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae unclassified 4.1 6.7 4.2 5.1 6.3 3.9 0.0000 0.0182 0.1189 0.0189 0.0003 0.0003 0.0000 0.0000 0.0004 0.0509 0.1289 0.0209 0.0003 0.0009 0.0003 0.0007

Otu0001442 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae unclassified - 6.7 5.4 2.6 6.4 5.7 0.0004 0.0043 0.1508 0.0620 0.0003 0.0003 0.0000 0.0000 0.0008 0.0110 0.1683 0.0914 0.0000 0.0003 0.0000 0.0000

Otu0006080 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae unclassified 3.5 3.9 - - 3.2 2.1 0.0000 0.0115 0.0161 0.0037 0.0000 0.0000 0.0000 0.0000 0.0000 0.0105 0.0103 0.0040 0.0003 0.0000 0.0000 0.0000

Otu0007269 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae unclassified - - 4.6 - 3.5 5.3 0.0000 0.0000 0.0018 0.0265 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0127 0.0430 0.0000 0.0000 0.0000 0.0000

Otu0007422 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae unclassified - - 3.3 - - 3.7 0.0000 0.0000 0.0019 0.0092 0.0000 0.0003 0.0000 0.0000 0.0003 0.0025 0.0070 0.0178 0.0000 0.0003 0.0003 0.0000

Otu0010126 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae unclassified - 3.8 - - 3.9 - 0.0000 0.0047 0.0152 0.0044 0.0000 0.0000 0.0000 0.0000 0.0000 0.0023 0.0178 0.0025 0.0000 0.0000 0.0000 0.0000

Otu0011631 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae unclassified - 3.2 - - 3.4 - 0.0000 0.0005 0.0098 0.0034 0.0000 0.0000 0.0000 0.0000 0.0000 0.0002 0.0119 0.0049 0.0000 0.0000 0.0000 0.0000

Otu0010074 Spirochaetes Spirochaetia Spirochaetales unclassified unclassified - - 3.3 - - 4.9 0.0000 0.0000 0.0066 0.0097 0.0000 0.0000 0.0000 0.0000 0.0000 0.0009 0.0059 0.0338 0.0000 0.0000 0.0000 0.0000

Otu0000425 unclassified unclassified unclassified unclassified unclassified - - - - - 2.0 0.0592 0.0555 0.0628 0.0168 0.0395 0.0414 0.0668 0.0660 0.0057 0.0039 0.0078 0.0244 0.0033 0.0129 0.0106 0.0044

Otu0000683 unclassified unclassified unclassified unclassified unclassified - 5.9 4.6 4.2 5.6 4.8 0.0000 0.0125 0.0660 0.0248 0.0000 0.0000 0.0000 0.0000 0.0000 0.0208 0.0584 0.0302 0.0003 0.0009 0.0003 0.0000

Otu0000861 unclassified unclassified unclassified unclassified unclassified - 5.6 8.1 3.8 6.1 8.3 0.0000 0.0071 0.0559 0.3066 0.0003 0.0022 0.0006 0.0000 0.0008 0.0258 0.1318 0.5736 0.0009 0.0009 0.0032 0.0009

Otu0000908 unclassified unclassified unclassified unclassified unclassified 2.2 2.5 2.8 - 2.4 - 0.0075 0.0388 0.0467 0.0559 0.0090 0.0087 0.0079 0.0079 0.0067 0.0383 0.0404 0.0385 0.0024 0.0111 0.0065 0.0102

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Otu0001250 unclassified unclassified unclassified unclassified unclassified 7.3 5.5 3.0 7.4 5.5 - 0.0000 0.1793 0.0504 0.0077 0.0003 0.0000 0.0000 0.0000 0.0000 0.2007 0.0549 0.0047 0.0003 0.0006 0.0000 0.0000

Otu0001312 unclassified unclassified unclassified unclassified unclassified - - - - - 2.5 0.0106 0.0154 0.0248 0.0614 0.0074 0.0168 0.0091 0.0232 0.0030 0.0076 0.0086 0.0203 0.0003 0.0024 0.0014 0.0024

Otu0001318 unclassified unclassified unclassified unclassified unclassified - - 5.2 - 4.7 7.8 0.0000 0.0003 0.0047 0.0410 0.0003 0.0006 0.0003 0.0000 0.0000 0.0005 0.0307 0.2574 0.0000 0.0003 0.0004 0.0003

Otu0001684 unclassified unclassified unclassified unclassified unclassified - 6.6 8.2 - 6.2 5.4 0.0000 0.0003 0.1101 0.3421 0.0000 0.0000 0.0004 0.0000 0.0000 0.0000 0.0883 0.0464 0.0000 0.0000 0.0000 0.0000

Otu0001950 unclassified unclassified unclassified unclassified unclassified 6.2 5.7 4.0 5.6 5.4 3.4 0.0000 0.0801 0.0583 0.0179 0.0000 0.0000 0.0000 0.0000 0.0004 0.0729 0.0682 0.0138 0.0000 0.0003 0.0000 0.0000

Otu0002067 unclassified unclassified unclassified unclassified unclassified - - 6.1 - - 8.1 0.0000 0.0007 0.0000 0.0772 0.0003 0.0000 0.0003 0.0000 0.0000 0.0006 0.0007 0.3087 0.0003 0.0000 0.0000 0.0000

Otu0002088 unclassified unclassified unclassified unclassified unclassified - 6.0 6.5 - 6.3 4.0 0.0012 0.0015 0.1417 0.1852 0.0011 0.0006 0.0014 0.0016 0.0000 0.0008 0.0968 0.0175 0.0006 0.0000 0.0003 0.0000

Otu0002199 unclassified unclassified unclassified unclassified unclassified - 4.5 4.9 - 6.6 3.7 0.0000 0.0003 0.0249 0.0318 0.0000 0.0000 0.0000 0.0000 0.0004 0.0002 0.1565 0.0188 0.0000 0.0000 0.0000 0.0000

Otu0002412 unclassified unclassified unclassified unclassified unclassified 3.4 - - - - - 0.0000 0.0112 0.0007 0.0003 0.0052 0.0003 0.0027 0.0000 0.0004 0.0019 0.0000 0.0015 0.0093 0.0011 0.0067 0.0073

Otu0002469 unclassified unclassified unclassified unclassified unclassified 3.1 - - - - - 0.0007 0.0149 0.0046 0.0025 0.0084 0.0000 0.0050 0.0163 0.0019 0.0000 0.0000 0.0003 0.0006 0.0000 0.0000 0.0003

Otu0002707 unclassified unclassified unclassified unclassified unclassified 6.1 4.4 - 5.2 3.5 - 0.0000 0.0782 0.0239 0.0022 0.0000 0.0000 0.0000 0.0000 0.0007 0.0709 0.0214 0.0010 0.0003 0.0000 0.0000 0.0000

Otu0002843 unclassified unclassified unclassified unclassified unclassified - - 4.5 - 4.4 6.8 0.0000 0.0000 0.0035 0.0234 0.0000 0.0000 0.0000 0.0000 0.0000 0.0006 0.0250 0.1285 0.0000 0.0003 0.0004 0.0000

Otu0003049 unclassified unclassified unclassified unclassified unclassified - 2.6 4.5 - 4.0 5.4 0.0016 0.0035 0.0164 0.0596 0.0003 0.0012 0.0004 0.0016 0.0004 0.0036 0.0246 0.0603 0.0006 0.0000 0.0004 0.0009

Otu0003128 unclassified unclassified unclassified unclassified unclassified - - 4.6 - 2.1 5.7 0.0007 0.0007 0.0019 0.0428 0.0003 0.0003 0.0000 0.0000 0.0018 0.0008 0.0124 0.1420 0.0009 0.0006 0.0000 0.0010

Otu0003503 unclassified unclassified unclassified unclassified unclassified - - 2.6 - - - 0.0013 0.0075 0.0031 0.0132 0.0021 0.0036 0.0020 0.0016 0.0008 0.0055 0.0015 0.0073 0.0010 0.0021 0.0023 0.0026

Otu0003585 unclassified unclassified unclassified unclassified unclassified - - 2.9 - 3.6 4.3 0.0019 0.0074 0.0102 0.0188 0.0024 0.0015 0.0028 0.0017 0.0003 0.0073 0.0177 0.0283 0.0018 0.0015 0.0011 0.0003

Otu0003898 unclassified unclassified unclassified unclassified unclassified - - - - 2.7 4.3 0.0004 0.0020 0.0046 0.0124 0.0000 0.0000 0.0004 0.0032 0.0015 0.0046 0.0166 0.0484 0.0015 0.0029 0.0018 0.0020

Otu0004053 unclassified unclassified unclassified unclassified unclassified - - - - - 2.2 0.0042 0.0045 0.0050 0.0034 0.0035 0.0056 0.0059 0.0065 0.0003 0.0008 0.0008 0.0058 0.0006 0.0003 0.0004 0.0004

Otu0004115 unclassified unclassified unclassified unclassified unclassified - - 2.6 - 2.1 4.3 0.0010 0.0005 0.0025 0.0115 0.0003 0.0012 0.0008 0.0020 0.0026 0.0065 0.0149 0.0661 0.0031 0.0037 0.0015 0.0043

Otu0004198 unclassified unclassified unclassified unclassified unclassified - - 4.8 - - 7.2 0.0000 0.0000 0.0000 0.0297 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.1687 0.0000 0.0000 0.0004 0.0000

Otu0004370 unclassified unclassified unclassified unclassified unclassified 5.5 5.4 - 4.5 4.3 - 0.0000 0.0492 0.0480 0.0007 0.0000 0.0000 0.0000 0.0000 0.0000 0.0267 0.0237 0.0004 0.0000 0.0000 0.0004 0.0000

Otu0004445 unclassified unclassified unclassified unclassified unclassified - - - - - 2.6 0.0021 0.0029 0.0040 0.0129 0.0022 0.0030 0.0031 0.0024 0.0012 0.0023 0.0043 0.0120 0.0009 0.0026 0.0014 0.0007

Otu0004762 unclassified unclassified unclassified unclassified unclassified - - - - - 3.7 0.0013 0.0013 0.0013 0.0048 0.0026 0.0024 0.0012 0.0032 0.0011 0.0017 0.0008 0.0274 0.0000 0.0011 0.0027 0.0014

Otu0004791 unclassified unclassified unclassified unclassified unclassified 3.0 5.1 3.7 3.5 5.1 3.4 0.0000 0.0080 0.0376 0.0123 0.0000 0.0000 0.0000 0.0000 0.0003 0.0168 0.0517 0.0148 0.0000 0.0000 0.0000 0.0000

Otu0005239 unclassified unclassified unclassified unclassified unclassified - - 2.6 - - 3.6 0.0007 0.0000 0.0000 0.0096 0.0010 0.0000 0.0012 0.0008 0.0055 0.0036 0.0210 0.0750 0.0019 0.0006 0.0021 0.0000

Otu0005777 unclassified unclassified unclassified unclassified unclassified - - 2.1 - - - 0.0004 0.0006 0.0034 0.0050 0.0009 0.0019 0.0004 0.0000 0.0019 0.0025 0.0100 0.0069 0.0006 0.0026 0.0029 0.0028

Otu0006034 unclassified unclassified unclassified unclassified unclassified - - 2.7 - - - 0.0008 0.0018 0.0052 0.0096 0.0000 0.0024 0.0021 0.0016 0.0000 0.0002 0.0020 0.0116 0.0009 0.0017 0.0008 0.0003

Otu0006705 unclassified unclassified unclassified unclassified unclassified - - 3.0 - - 5.1 0.0008 0.0000 0.0006 0.0127 0.0003 0.0000 0.0007 0.0000 0.0003 0.0005 0.0004 0.0509 0.0009 0.0006 0.0000 0.0000

Otu0007045 unclassified unclassified unclassified unclassified unclassified - - - - 2.1 - 0.0000 0.0003 0.0012 0.0038 0.0015 0.0000 0.0008 0.0000 0.0011 0.0000 0.0088 0.0055 0.0003 0.0015 0.0004 0.0000

Otu0007121 unclassified unclassified unclassified unclassified unclassified - - 4.2 - - - 0.0000 0.0000 0.0000 0.0191 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0054 0.0000 0.0000 0.0000 0.0000

Otu0007125 unclassified unclassified unclassified unclassified unclassified - 4.1 4.6 - 3.8 4.1 0.0000 0.0025 0.0191 0.0261 0.0000 0.0000 0.0000 0.0000 0.0000 0.0031 0.0157 0.0182 0.0000 0.0000 0.0000 0.0000

Otu0007508 unclassified unclassified unclassified unclassified unclassified - - 2.9 - - 4.7 0.0000 0.0000 0.0015 0.0074 0.0000 0.0000 0.0000 0.0000 0.0000 0.0006 0.0100 0.0288 0.0000 0.0000 0.0000 0.0004

Otu0007527 unclassified unclassified unclassified unclassified unclassified - - 3.8 - - 3.1 0.0006 0.0009 0.0045 0.0219 0.0009 0.0012 0.0014 0.0007 0.0000 0.0007 0.0015 0.0088 0.0000 0.0006 0.0011 0.0006

Otu0007649 unclassified unclassified unclassified unclassified unclassified - 3.9 - - 3.0 - 0.0000 0.0063 0.0163 0.0048 0.0000 0.0000 0.0000 0.0000 0.0000 0.0042 0.0088 0.0018 0.0000 0.0003 0.0000 0.0000

Otu0008269 unclassified unclassified unclassified unclassified unclassified 2.6 3.7 2.4 3.2 3.7 2.2 0.0003 0.0078 0.0185 0.0063 0.0000 0.0000 0.0000 0.0000 0.0000 0.0103 0.0153 0.0043 0.0003 0.0000 0.0000 0.0000

Otu0008587 unclassified unclassified unclassified unclassified unclassified - - - - - 2.2 0.0011 0.0007 0.0004 0.0028 0.0003 0.0003 0.0011 0.0000 0.0004 0.0004 0.0016 0.0057 0.0000 0.0009 0.0010 0.0006

Otu0008653 unclassified unclassified unclassified unclassified unclassified - 2.7 2.1 - - - 0.0007 0.0087 0.0104 0.0064 0.0000 0.0000 0.0000 0.0004 0.0000 0.0042 0.0039 0.0007 0.0003 0.0000 0.0000 0.0000

Otu0009034 unclassified unclassified unclassified unclassified unclassified - - - - 3.6 5.1 0.0000 0.0006 0.0023 0.0070 0.0000 0.0000 0.0000 0.0000 0.0000 0.0038 0.0139 0.0396 0.0000 0.0003 0.0003 0.0000

Otu0009230 unclassified unclassified unclassified unclassified unclassified - - 2.7 - - 3.1 0.0014 0.0009 0.0007 0.0151 0.0012 0.0006 0.0000 0.0000 0.0008 0.0000 0.0016 0.0143 0.0006 0.0003 0.0000 0.0004

Otu0009578 unclassified unclassified unclassified unclassified unclassified 3.6 - - 3.7 - - 0.0000 0.0132 0.0017 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0139 0.0058 0.0000 0.0000 0.0000 0.0000 0.0000

Otu0009597 unclassified unclassified unclassified unclassified unclassified - 3.1 - 3.4 4.0 - 0.0000 0.0100 0.0086 0.0022 0.0000 0.0000 0.0000 0.0000 0.0000 0.0119 0.0179 0.0011 0.0000 0.0003 0.0000 0.0000

Otu0009937 unclassified unclassified unclassified unclassified unclassified - - - - - 2.5 0.0007 0.0003 0.0003 0.0023 0.0000 0.0003 0.0016 0.0000 0.0004 0.0002 0.0007 0.0071 0.0003 0.0000 0.0003 0.0000

Otu0010053 unclassified unclassified unclassified unclassified unclassified - - 3.2 - - 3.7 0.0000 0.0005 0.0016 0.0090 0.0000 0.0000 0.0008 0.0000 0.0000 0.0012 0.0043 0.0138 0.0000 0.0006 0.0008 0.0000

Otu0010349 unclassified unclassified unclassified unclassified unclassified - - - - - 2.8 0.0010 0.0000 0.0007 0.0020 0.0000 0.0000 0.0003 0.0000 0.0000 0.0004 0.0020 0.0066 0.0000 0.0003 0.0000 0.0007

Otu0011275 unclassified unclassified unclassified unclassified unclassified - - 4.7 - - 3.3 0.0000 0.0000 0.0007 0.0288 0.0003 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0101 0.0000 0.0000 0.0000 0.0003

Otu0011424 unclassified unclassified unclassified unclassified unclassified - - - - - 2.8 0.0008 0.0003 0.0000 0.0008 0.0013 0.0000 0.0004 0.0004 0.0007 0.0003 0.0007 0.0121 0.0012 0.0009 0.0015 0.0007

Otu0011516 unclassified unclassified unclassified unclassified unclassified - 3.2 - - 3.3 - 0.0004 0.0042 0.0131 0.0026 0.0000 0.0000 0.0000 0.0000 0.0000 0.0058 0.0112 0.0027 0.0000 0.0000 0.0000 0.0000

Otu0011780 unclassified unclassified unclassified unclassified unclassified - - - - - 2.4 0.0003 0.0003 0.0043 0.0040 0.0011 0.0009 0.0000 0.0004 0.0000 0.0005 0.0037 0.0048 0.0003 0.0003 0.0007 0.0000

Otu0013665 unclassified unclassified unclassified unclassified unclassified - - 2.6 - - - 0.0008 0.0014 0.0018 0.0093 0.0009 0.0006 0.0022 0.0007 0.0000 0.0003 0.0012 0.0011 0.0000 0.0000 0.0006 0.0004

Otu0013809 unclassified unclassified unclassified unclassified unclassified - - - - - 3.8 0.0000 0.0000 0.0004 0.0081 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0015 0.0156 0.0000 0.0000 0.0000 0.0000

Otu0013850 unclassified unclassified unclassified unclassified unclassified - - - - 2.3 3.4 0.0000 0.0003 0.0029 0.0027 0.0003 0.0000 0.0004 0.0000 0.0004 0.0008 0.0068 0.0151 0.0000 0.0000 0.0003 0.0000

Otu0015202 unclassified unclassified unclassified unclassified unclassified - - - - - - 0.0000 0.0003 0.0000 0.0019 0.0000 0.0000 0.0000 0.0000 0.0004 0.0006 0.0004 0.0093 0.0000 0.0009 0.0003 0.0000

Otu0018174 unclassified unclassified unclassified unclassified unclassified - 2.6 3.7 - - - 0.0000 0.0000 0.0059 0.0135 0.0003 0.0000 0.0000 0.0000 0.0000 0.0000 0.0018 0.0010 0.0000 0.0000 0.0000 0.0000

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Otu0019281 unclassified unclassified unclassified unclassified unclassified - - - - - 2.5 0.0000 0.0000 0.0004 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0004 0.0052 0.0000 0.0000 0.0004 0.0000

Otu0020301 unclassified unclassified unclassified unclassified unclassified - - - - - 2.6 0.0000 0.0000 0.0003 0.0005 0.0000 0.0003 0.0000 0.0000 0.0000 0.0000 0.0004 0.0056 0.0000 0.0003 0.0000 0.0007

Otu0021846 unclassified unclassified unclassified unclassified unclassified - - 2.6 - - - 0.0000 0.0000 0.0022 0.0055 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0015 0.0051 0.0000 0.0003 0.0000 0.0000

Otu0022246 unclassified unclassified unclassified unclassified unclassified - - - - - 2.6 0.0000 0.0000 0.0000 0.0021 0.0000 0.0000 0.0000 0.0000 0.0000 0.0004 0.0004 0.0059 0.0000 0.0000 0.0000 0.0000

Otu0003550 Verrucomicrobia Opitutae Puniceicoccales Puniceicoccaceae unclassified - - 5.0 - - 5.7 0.0007 0.0003 0.0034 0.0567 0.0013 0.0003 0.0010 0.0008 0.0004 0.0017 0.0068 0.0758 0.0012 0.0006 0.0011 0.0006

Otu0023706 Verrucomicrobia Opitutae Puniceicoccales Puniceicoccaceae unclassified - - - - - 2.5 0.0004 0.0000 0.0000 0.0029 0.0000 0.0003 0.0000 0.0000 0.0000 0.0000 0.0000 0.0052 0.0000 0.0000 0.0000 0.0000

Otu0003230 Verrucomicrobia Subdivision3 unclassified unclassified unclassified - - - - - 2.7 0.0019 0.0035 0.0059 0.0056 0.0013 0.0006 0.0000 0.0103 0.0004 0.0012 0.0036 0.0087 0.0137 0.0088 0.0006 0.0006

Otu0016313 Verrucomicrobia unclassified unclassified unclassified unclassified - - - - - 3.1 0.0000 0.0003 0.0000 0.0038 0.0000 0.0000 0.0000 0.0000 0.0000 0.0002 0.0008 0.0083 0.0000 0.0000 0.0000 0.0003

Representative sequence

OTU Name Sequence

Otu0007996 M01867_242_000000000_ATWYL_1_2109_9712_16254 .GGG--G-A-A--TT-TT--TC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-A-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-C-T--CC-------------------------TG-TC-G-A--C-TGG----G--A-A--G---AA--TGC-A---C-A-C----G--A-C-----------------C--T----------------------------AA--------------------------------T---A----C-------------G-TC---G-CTG-T-G--T-T-G-A-C-T-----G-T---A-C-CG--------G-T-G-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-T-C-GGAA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TC-G-T-T----------------------TA----G-T-C-C------------C-G-T--G--TG--A-AA-GC--C-C-TC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-A-G-G-A--A-C----G-G--C-G---C--G--------------G--GA-A-A---C---------------T--G-G--CG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-GG------GA-G-A--------------G-G-G-A---AG-C--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GC-AG--AT-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-C------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----T-AG--GG-G--AG-C-A-AA--CG

Otu0000628 M01867_242_000000000_ATWYL_1_1101_14433_22469 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------C-G-T--A---AA-A-C-CC--------------------------TG-TC-G-T--C-GGG----G--A-C--G---AA--GGT-A---A-T-T--C-G--G-------------------T--T----------------------------AA--------------------------------C---A----A---------------CT---G-AGT-T-GC-T-T-G-A-C-G-----G-T---A-C-CC--------G-G-A-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TT-T-T-T----------------------AA----G-T-C-A------------G-A-T--G--TG--T-AA-TC--C-C-CG-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-G-G-G-A--A-C----T-G--C-A---T--C--------------T--GA-G-A---C---------------T--G-G--AA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-TG------GA-G-A--------------G-G-G-T---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-C-A-C---CTG-----G--AC-A-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-T----G-TG--GG-T--AG-C-A-AA--CA

Otu0008421 M01867_242_000000000_ATWYL_1_2107_21464_18363 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------C-G-T--A---AA-A-C-CC--------------------------TG-TC-G-T--C-GGG----G--A-C--G---AA--GGT-G---G-C-T--T-A--G-------------------T--T----------------------------AA--------------------------------C---A----G---------------CT---G-AGT-T-GC-T-T-G-A-C-G-----G-T---A-C-CC--------G-G-A-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TT-T-T-T----------------------AA----G-T-C-A------------G-A-T--G--TG--T-AA-TC--C-C-CG-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-G-G-G-A--A-C----T-G--C-A---T--C--------------T--GA-G-A---C---------------T--G-G--AA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-TG------GA-G-A--------------G-G-G-T---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-C-A-C---CTG-----G--AC-A-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-T----G-TG--GG-T--AG-C-A-AA--CA

Otu0002518 M01867_242_000000000_ATWYL_1_2110_24479_12826 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-A-C-CC--------------------------TG-TC-G-T--C-AGG----G--A-C--G---AA--GGT-T---G-T-G--G-C--C-------------------T--T----------------------------AA--------------------------------T---A----C---------------GG---T-TAC-A-AC-T-T-G-A-C-G-----G-T---A-C-CT--------G-G-A-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TC-T-T-T----------------------AA----G-T-C-G------------G-A-T--G--TG--C-AA-TC--C-C-CG-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-G-G-G-A--A-C----T-G--C-A---T--C--------------C--GA-A-A---C---------------T--G-G--GG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-TG------GA-G-A--------------G-G-G-T---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-C-A-C---CTG-----G--AC-A-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-T----G-TG--GG-T--AG-C-A-AA--CA

Otu0001732 M01867_242_000000000_ATWYL_1_1109_11803_9455 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-A-C-CC--------------------------TG-TC-G-T--C-AGG----G--A-C--G---AA--GGG-T---T-G-C--G-A--C-------------------C--T----------------------------AA--------------------------------T---A----C---------------GT---C-GTG-G-CC-T-T-G-A-C-G-----G-T---A-C-CT--------G-G-A-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-GT-C-G-T----------------------AA----G-T-A-G------------G-A-T--G--TG--T-AA-TC--C-C-CG-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-G-G-G-A--A-C----T-G--C-A---T--C--------------C--TA-G-A---C---------------T--G-C--GA--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-TG------GA-G-A--------------G-G-G-T---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-C-A-C---CTG-----G--AC-A-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-T----G-TG--GG-T--AG-C-A-AA--CA

Otu0002001 M01867_242_000000000_ATWYL_1_1107_26645_10504 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-A-C-CC--------------------------TG-TC-G-T--C-AGG----G--A-C--G---AA--GGT-A---T-G-G--T-T--C-------------------T--T----------------------------AA--------------------------------T---A----C---------------GG---A-TCA-T-GC-T-T-G-A-C-G-----G-T---A-C-CT--------G-G-A-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TT-T-G-T----------------------AA----G-T-A-G------------G-A-T--G--TG--T-AA-TC--C-C-CG-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-G-G-G-A--A-C----T-G--C-A---T--C--------------C--TA-G-A---C---------------T--G-C--AA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-TG------GA-G-A--------------G-G-G-T---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-C-A-C---CTG-----G--AC-A-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0002350 M01867_242_000000000_ATWYL_1_2104_8664_14807 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-A-C-CC--------------------------TG-TC-G-T--C-AGG----G--A-C--G---AA--GGT-T---G-G-G--A-T--C-------------------C--T----------------------------AA--------------------------------T---A----G---------------GG---T-TTC-A-GC-T-T-G-A-C-G-----G-T---A-C-CT--------G-G-A-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TT-T-G-T----------------------AA----G-T-A-G------------G-A-T--G--TG--T-AA-TC--C-C-CG-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-G-G-G-A--A-C----T-G--C-A---T--C--------------C--TA-G-A---C---------------T--G-C--AA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-TG------GA-G-A--------------G-G-G-T---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-C-A-C---CTG-----G--AC-A-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0012233 M01867_242_000000000_ATWYL_1_2102_23341_24204 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------C-G-T--A---AA-A-C-CC--------------------------TG-TC-G-T--C-AGG----G--A-C--G---AA--GGT-T---G-C-A--T-G--T-------------------C--T----------------------------AA--------------------------------T---A----G---------------AT---G-TGC-A-GC-T-T-G-A-C-G-----G-T---A-C-CT--------G-G-A-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-GT-C-T-T----------------------AA----G-T-G-A------------G-A-T--G--TG--T-AA-TC--C-C-CG-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-G-G-G-A--A-C----T-G--C-A---T--C--------------T--CA-G-A---C---------------T--G-G--GG--C-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-TG------GA-G-A--------------G-G-G-T---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-C-A-C---CTG-----G--AC-A-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0005421 M01867_242_000000000_ATWYL_1_2114_17518_27503 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--TC---------------------------GA-G--A--G-A--C-T-G-A-A-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------A-G--A--A--G-G-CC---T-----TA-TG-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TT-TT-G-T--A-CGG----G--A-G--A---AA-AAAG-A---C-A-G--T---------------------------C----------------------------GC--------------------------------G-----------------------------C-ATTGT-CA-T-T-G-A-T-A-----G-T---A-C-CG--------T-A-A-G---------A-A-----------T--AA-------------GG-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-TT-A-T-T----------------------AA----G-T-C-A------------G-T-G--T--TG--A-AA-GA--C-T-AA-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-T-A-G-A--A-G----T-G--G-C---A--T--------------T--GA-T-A---C---------------T--G-G--TA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AG------AT-G-A--------------A-G-T-G---GG-C--------------GG--A--ATG-----------T-G-T-C-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-AT-AG--AT-A-TG-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA-T--T--GC-GAA-G--G-C----A-----G--C-T-C-A---CTA-----A--GT-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0000121 M01867_242_000000000_ATWYL_1_1101_19185_12310 .GAG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--T-G-CC---C-----TA-TG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-G-T--A-CGG----G--A-C--G---AA-ACTC-T---T-G-G--T---------------------------C----------------------------GT--------------------------------G-----------------------------A-CCA-G-GA-T-T-G-A-C-T-----G-T---A-C-CG--------T-G-C-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TC-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-AT-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-GC--C-T-GT-G-G-----------------------------------------------------CT-C-AA-----------C-C-A-T-A-G-A--A-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-AG------TT-G-A--------------A-G-T-G---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----AA-T--T--GC-GAA-G--G-C----A-----G--C-T-C-A---CTA-----A--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000312 M01867_242_000000000_ATWYL_1_1101_10372_2681 .GAG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--T-G-CC---C-----TA-TG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-G-T--A-CGG----G--A-C--G---AA-ACTC-C---T-G-G--T---------------------------C----------------------------GT--------------------------------G-----------------------------A-CCG-G-GA-T-T-G-A-C-T-----G-T---A-C-CG--------T-G-C-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-GC-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-GC--C-T-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TG--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-AG------TT-G-A--------------A-G-T-G---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----AA-T--T--GC-GAA-G--G-C----A-----G--C-T-C-A---CTA-----A--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000641 M01867_242_000000000_ATWYL_1_1102_22250_16269 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-CCC-G-C-G-T-----G-A-A--G--GA--------------------------A-G--A--A--A-G-CG---C-----TA-TG-------C-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-C-C--G-GAG----G--A-A--T---AA-AATC-C---C-G-C--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-GCG-G-GA-C-T-G-A-A-G-----G-T---A-C-TC--------C-G-G-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-TT-A-A-T----------------------AA----G-T-C-A------------G-C-G--G--TG--A-AA-AC--C-T-TC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-A-G-A--C-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GG------TT-A-A--------------G-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----AA-T--T--GC-GAA-G--G-C----A-----G--C-T-T-A---CTG-----A--GC-C-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0000945 M01867_242_000000000_ATWYL_1_1102_21244_17545 .GAG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--T-G-CC---C-----TA-TG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-G-T--A-CGG----G--A-C--G---AA-ACTC-C---T-G-G--T---------------------------C----------------------------GT--------------------------------G-----------------------------A-CCG-G-GA-T-T-G-A-C-G-----G-T---A-C-CG--------T-A-C-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-GT-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--T-T-GC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-T-A-A-A--A-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-AG------TT-G-A--------------A-G-T-G---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----AA-T--T--GC-GAA-G--G-C----A-----G--C-T-C-A---CTA-----A--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001124 M01867_242_000000000_ATWYL_1_1101_11543_19224 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-CCC-G-C-G-T-----G-A-A--G--GA--------------------------A-G--A--A--G-G-CG---C-----TA-TG-------C-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-C-C--A-GGG----G--A-A--T---AA-AATT-T---T-G-T--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-ACG-G-AA-T-T-G-A-A-G-----G-T---A-C-CC--------T-G-G-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-CA-G-G-C-G---------------G--A-AT-A-G-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-AC--C-T-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GG------TC-A-A--------------G-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA-T--T--GC-GAA-G--G-C----A-----G--C-T-T-G---CTG-----G--GC-C-A------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-G-AA--CA

Otu0001265 M01867_242_000000000_ATWYL_1_1105_26355_23838 .AGG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-A-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---T-----TA-TG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TT-TT-A-T--A-TGG----G--A-A--G---AA-ACCC-T---C-G-G--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-CCG-G-GG-C-T-G-A-C-G-----G-T---A-C-CA--------T-A-G-G---------A-A-----------T--AA-------------GG-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------CCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--G-TT-G-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--C-T-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--CA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AG------TT-G-A--------------G-G-T-G---GG-C--------------GG--A--ATT-----------T-G-T-C-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TG-----------------------------A-C---A-A--A-G-A-AC-A-CC----GA-T--C--GC-GAA-G--G-C----A-----G--T-T-C-A---CCA-----A--AC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0001780 M01867_242_000000000_ATWYL_1_1101_15564_26029 .GAG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--T-G-CC---C-----TA-TG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-G-T--A-TGG----G--A-C--G---AA-ACTC-T---T-G-G--T---------------------------C----------------------------GT--------------------------------G-----------------------------A-CCG-A-GA-T-T-G-A-C-G-----G-T---A-C-CG--------T-A-C-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-AT-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-GC--C-T-GC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-T-A-G-A--A-A----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-AG------TT-G-A--------------A-G-T-G---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----AA-T--T--GC-GAA-G--G-C----A-----G--C-T-C-A---CTA-----A--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001871 M01867_242_000000000_ATWYL_1_1110_26335_11435 .GAG--G-A-A--TA-TT--GG-TC-------AA-T-G-G--GC---------------------------GA-G--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-CCC-G-C-G-T-----G-C-A--G--GA--------------------------A-G--A--C--G-G-CC---C-----TA-TG-------G-G-T--------T-G-TA-A---A--C-T-GC--------------------------TT-TT-T-T--A-TGG----G--A-A--G---AA-AAAG-C---T-C-T--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-AGG-G-CA-C-T-G-A-C-G-----G-T---A-C-CA--------T-A-T-G---------A-AT-------------AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCG-A-G------------C--G--T---T--AT-CCGG--AT------TT-A--T-T--AG-GT-------------------TT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--A-CT-G-T-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--T-C-AG-AG------------------------------------------------------CT-T-AA-----------C-T-C-T-G-A-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-A--CA--G-C-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AG------TT-G-A--------------A-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--GA---------A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA--T-T--GC-GAA-G--G-C----A-----G--CT--T-A---CTA-----A--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0002159 M01867_242_000000000_ATWYL_1_1102_10421_20002 .AGG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-A-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---T-----TA-TG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TT-TT-G-T--A-CGG----G--A-A--G---AA-ACCC-T---C-G-A--A---------------------------C----------------------------GT--------------------------------G---T---------------------------TCG-G-GG-C-T-G-A-C-G-----G-T---A-C-CG--------T-A-A-G---------A-A-----------T--AA-------------GG-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G---GCG---------------G--A-TT-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--C-T-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-TG------TT-G-A--------------G-G-T-G---GG-C--------------GG--A--ATT-----------T-G-T-C-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TG-----------------------------A-C---A-A--A-G-A-AC-A-CC----AA-T--C--GC-GAA-G--G-C----A-----G--T-T-C-A---CCA-----A--AC-A-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0002465 M01867_242_000000000_ATWYL_1_1112_24273_10964 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GT---------------------------GC-A--A--A-C--C-T-G-A-A-C-C-A---GC-C-----A--A-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-CT---C-----TA-GT-------A-G-T--------C-G-T--A---AA-C-T-TC--------------------------TT-TT-A-T--A-GGG----G--A-A--T---AA-TAGC-T---T-G-T--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-ACA-G-GT-A-T-G-A-A-T-----G-T---A-C-TC--------T-A-T-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-G-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--AG-GT-------------------TT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--A-TT-A-T-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--T-C-CT-C-G-----------------------------------------------------CT-T-AA-----------C-G-G-G-G-A-A--A-C----T-G--C-C---A--T--------------A--GA-T-A---C---------------T--G-A--TA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--A-----T-AG------TT-G-A--------------G-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----AA-T--T--GC-GTA-G--G-C----A-----G--C-T-T-A---CTA-----A--AC-T-A------------------------------------------------------------------------------T-T------A-T-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0002784 M01867_242_000000000_ATWYL_1_1101_21102_15467 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GA-G--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-TCC-G-C-G-T-----G-A-A--G--GA--------------------------A-G--A--A--G-G-CC---C-----TA-CG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-C-T--G-TAA----G--A-A--G---AA-AAGT-C---C-C-G--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-CGG-G-AT-G-T-G-A-C-G-----G-T---A-T-TA--------T-A-G-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-CA-G-G-T-G---------------G--T-TT-T-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-GT--C-T-GT-C-G-----------------------------------------------------CT-T-AA-----------C-G-A-T-A-G-G--A-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--AG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GG------AT-G-A--------------G-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-AT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-T-CC----GA-T--T--GC-GAA-G--G-C----A-----G--C-T-T-A---CTA-----A--TC-C-A------------------------------------------------------------------------------C-C------A-C-T--GA--CA---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0003134 M01867_242_000000000_ATWYL_1_1104_21775_16872 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GG-A--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------A-G--A--C--G-G-CC---C-----TA-CG-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TT-TT-C-C--G-GGG----G--A-A--G---AA-TAAT-C---C-C-G--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-CGG-GAG--A-C-G-A-C-G-----G-T---A-C-CC--------C-G-G-G---------A-A-----------T--AA-------------GG-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------CCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--G-CG-C-T-T----------------------AA----G-T-C-A------------G-C-G--G--TG--A-AA-TA--C-G-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-T-C-G-A--A-C----G-G--C-C---G--T--------------T--GA-T-A---C---------------T--G-G--GC--G-C-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-G-A-T--T-----C-GG------TC-G-A--------------G-G-C-G---GG-C--------------GG--A--ATG-----------C-G-A-C-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TG-----------------------------T-C---G-C--A-G-A-AC-G-CC----GA-T--T--GC-GAA-G--G-C----A-----G--C-TCG-C---CAG-----G--CC-G--------------------------------------------------------------------------------A-C------A-T-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0003549 M01867_242_000000000_ATWYL_1_1114_20702_11539 .AGG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-A-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---T-----TA-TG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TT-TT-A-T--A-TGG----G--A-A--G---AA-ACCC-T---C-G-G--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-CCG-G-GG-C-T-G-A-C-G-----G-T---A-C-CA--------T-A-A-G---------A-A-----------T--AA-------------GG-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------CCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--G-CT-T-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--C-A-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-T-G-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-AG------TT-G-A--------------G-G-T-G---GG-C--------------GG--A--ATT-----------T-G-T-C-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TG-----------------------------A-C---A-A--A-G-A-AC-A-CC----GA-T--C--GC-GAA-G--G-C----A-----G--T-T-C-A---CCA-----A--AC-T-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0004398 M01867_242_000000000_ATWYL_1_2106_6379_21485 .AGG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-A-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---T-----TA-TG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TT-TT-G-T--A-CGG----G--A-A--G---AA-ACCC-T---C-C-G--A---------------------------C----------------------------GT--------------------------------G---T---------------------------CGG-G-GG-C-T-G-A-C-G-----G-T---A-C-CG--------T-A-A-G---------A-A-----------T--AA-------------GG-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G---GCG---------------G--A-TT-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--C-T-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-TG------TT-G-A--------------G-G-T-G---GG-C--------------GG--A--ATT-----------T-G-T-C-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TG-----------------------------A-C---A-A--A-G-A-AC-A-CC----AA-T--C--GC-GAA-G--G-C----A-----G--T-T-C-A---CCA-----A--AC-A-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0004412 M01867_242_000000000_ATWYL_1_1114_11380_26934 .AAG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-C-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--C--G-G-CC---C-----TA-GT-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TT-TT-A-T--A-CGG----G--A-A--A---AA-ACCC-C---T-G-G--T---------------------------C----------------------------GT--------------------------------GC----------------------------A-TCA-G-GG-C-T-G-A-T-G-----G-T---A-C-CG--------T-A-A-G---------A-A-----------T--AA-------------GG-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AT------TC-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-CA-G-G-C-----------------G--GGAT-A-A-T----------------------AA----G-T-T-A------------G-T-G--G--TG--A-AA-GC--C-T-AC-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-T----T-G--C-C---A--T--------------T--AA-T-A---C---------------T--G-T--TA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AG------TT-G-A--------------A-G-T-G---GG-C--------------GG--A--ATG-----------T-G-T-C-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TG-----------------------------A-C---A-C--A-G-A-AC-G-CC-----G-AT-A--GC-GAA-G--G-T----A-----G--C-T-C-A---CTA-----A--GC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-C--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004457 M01867_242_000000000_ATWYL_1_2104_7853_14669 .GAG--G-A-A--TA-TT--GG-TC-------AA-T-G-G--GC---------------------------GG-G--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-CCC-G-C-G-T-----G-C-A--G--GA--------------------------A-G--A--C--G-G-CC---C-----TA-TG-------G-G-T--------T-G-TA-A---A--C-T-GC--------------------------TT-TT-T-T--A-TGG----G--A-A--G---AA-AAAG-C---T-C-T--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-AGG-G-CA-C-T-G-A-C-G-----G-T---A-C-CA--------T-A-T-G---------A-AT-------------AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCG-A-G------------C--G--T---T--AT-CCGG--AT------TT-A--T-T--AG-GT-------------------TT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--A-TT-G-T-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--T-C-AC-AG------------------------------------------------------CT-C-AA-----------C-T-G-T-G-A-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-G--TA--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AG------TT-G-A--------------A-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--GA---------A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA--T-T--GC-GAA-G--G-C----A-----G--CT--T-A---CTA-----A--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0005429 M01867_242_000000000_ATWYL_1_1111_7574_13195 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GT---------------------------GC-A--A--A-C--C-T-G-A-A-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--C--G-G-CT---C-----TC-GT-------A-G-T--------T-G-T--A---AA-C-C-TC--------------------------TT-TT-G-T--A-TGG----G--A-A--A---AA-ACTC-C---T-G-A--T---------------------------C----------------------------GT--------------------------------G-----------------------------AATCG-G-GA-C-T-G-A-T-T-----G-T---A-C-CA--------T-A-G-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-GG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--AG-GT-------------------TT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--A-TG-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--T-C-CC-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-G-G-A-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TT--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--G-----T-AG------TT-G-A--------------G-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA-T--T--GC-GAA-G--G-C----A-----G--C-T-T-A---CTA-----A--AC-T-A------------------------------------------------------------------------------C-T------A-T-T--GA--CG---C----------------------------T-G--A-TA--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0005536 M01867_242_000000000_ATWYL_1_1104_16429_19704 .AGG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-C-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---C-----TA-GT-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TT-TT-G-T--A-CGG----G--A-A--A---AA-ACCC-C---C-C-G--A---------------------------C----------------------------GT--------------------------------G---T---------------------------CGG-G-GG-T-T-G-A-T-G-----G-T---A-C-TG--------T-A-A-G---------A-A-----------T--AA-------------GG-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G---GCG---------------G--A-TT-T-G-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--C-T-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-C----T-G--C-C---A--T--------------A--GA-T-A---C---------------T--G-C--AA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AG------TT-G-G--------------C-G-T-G---GA-T--------------GG--A--ACG-----------T-G-T-C-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TG-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA-T--C--GC-GAA-G--G-C----A-----G--T-T-C-A---CGA-----A--AC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0006621 M01867_242_000000000_ATWYL_1_1104_25950_7776 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-CCC-G-C-G-T-----G-C-A--G--GA--------------------------C-G--A--C--G-G-CC---C-----TA-CG-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TT-TT-G-T--A-ATA----G--A-A--G----A-AACC-G---G-T-T--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-AAC-C-GA-C-T-G-A-C-A-----G-T---A-T-AT--------T-A-T-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TC-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--G-TT-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TT--C-T-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-GG------TT-G-A--------------A-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA-T--T--GC-GTA-G--G-C----A-----G--C-T-T-A---CTA-----A--AC-C-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----A-TG--GG-G--AT-C-A-AA--CA

Otu0007366 M01867_242_000000000_ATWYL_1_2101_4389_22290 .GAG--G-A-A--TA-TT--GG-TC-------AA-T-G-G--GC---------------------------GA-G--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-CCC-G-C-G-T-----G-C-A--G--GA--------------------------A-G--A--C--G-G-CC---C-----TA-TG-------G-G-T--------T-G-TA-A---A--C-T-GC--------------------------TT-TT-T-T--A-TGG----G--A-A--G---AA-AAAG-C---C-C-T--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-AGG-G-CA-C-T-G-A-C-G-----G-T---A-C-CA--------T-A-T-G---------A-AT-------------AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCG-A-G------------C--G--T---T--AT-CCGG--AT------TT-A--T-T--AG-GT-------------------TT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--A-CG-A-T-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--T-C-AC-AG------------------------------------------------------CT-T-AA-----------C-T-G-T-G-A-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-G--TT--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AG------TT-G-A--------------A-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--GA---------A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA--T-T--GC-GAA-G--G-C----A-----G--CT--T-A---CTA-----A--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0008764 M01867_242_000000000_ATWYL_1_1105_20215_9824 .GAG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--AC---------------------------GA-G--A--G-T--C-T-G-A-C-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------A-G--A--C--G-G-CC---C-----TA-TG-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TT-TT-T-T--T-ATG----G--A-G--T---AA-AATT-A---C-C-T--A---------------------------T----------------------------GT--------------------------------A-----------------------------T-AGG-T-AT-C-T-G-C-A-A-----G-T---A-C-AT--------A-A-A-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--AG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-AC-A-G-T----------------------AA----G-T-C-A------------G-A-G--G--TG--A-AA-AC--A-A-GC-T-G-----------------------------------------------------CT-C-AA-----------C-A-G-C-G-T-G--C-T----T-G--C-C---T--T--------------T--GA-T-A---C---------------T--G-C--TG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----T-AT------AC-A-A--------------A-G-T-A---TG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-AT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA-T--A--GC-GAA-G--G-C----A-----G--C-A-T-A---CTG-----G--AA-T-A------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0008950 M01867_242_000000000_ATWYL_1_1105_18454_20750 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GA-G--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------A-G--A--C--G-G-CC---C-----TA-CG-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TT-TT-G-T--T-TGG----G--A-A--G---AA-AAGG-C---G-C-T--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-AGT-G-CA-C-T-G-A-C-G-----G-T---A-C-CA--------G-A-T-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--AG-GT-------------------TT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--A-AG-A-C-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--C-C-CC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-G-A--A-C----T-G--C-C---A--T--------------A--GA-T-A---C---------------T--G-G--TT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AG------TT-G-A--------------A-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA-T--T--GC-GAA-G--G-C----A-----G--C-T-T-G---CTA-----A--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0009674 M01867_242_000000000_ATWYL_1_1111_28083_15899 .AGG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-A-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-T--A--A--G-G-CC---T-----TA-TG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TT-TT-A-T--A-TGG----G--A-A--G---AA-ACCC-T---T-G-G--T---------------------------C----------------------------GT--------------------------------G-----------------------------A-CCG-A-GG-T-T-G-A-C-G-----G-T---A-C-CA--------T-A-G-G---------A-A-----------T--AA-------------GG-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------CCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-TT-G-A-T----------------------AA----G-T-C-A------------G-A-G--G--TG--A-AA-TC--C-A-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-T-G-A--A-C----T-G--C-C---T--T--------------T--GA-T-A---C---------------T--G-T--TA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-AG------TT-G-G--------------A-G-T-G---GA-T--------------GG--A--ATT-----------T-G-T-C-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TG-----------------------------A-C---A-A--A-G-A-AC-A-CC----AA-T--C--GC-GAA-G--G-C----A-----G--T-T-C-A---CTA-----A--AC-T-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0013428 M01867_242_000000000_ATWYL_1_1110_5155_8925 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--TC---------------------------GA-G--A--G-A--C-T-G-A-A-C-C-A---GC-C-----A--A-GCC-G-C-G-T-----G-C-A--G--GA--------------------------TAG--A--A--G-G-CC---C-----TA-TG-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TT-TT-G-T--A-CGG----G--A-A--G---AA-AAAG-A---G-C-T--A---------------------------C----------------------------GA--------------------------------G-----------------------------T-AGC-T-CC-T-T-G-C-C-G-----G-T---A-C-TG--------T-A-C-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-G-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--AG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--G-AA-A-C-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--C-T-AT-G-G-----------------------------------------------------CT-C-AA-----------C-C-A-T-A-G-C--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-G--TT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----T-AG------TT-G-A--------------G-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-T-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TG-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA-T--T--GC-GAA-G--G-C----A-----G--C-T-T-A---CTA-----A--AC-T-A------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-T--AG-C-G-AA--CA

Otu0025199 M04041_72_000000000_AUMPE_1_1103_15833_12354 .AGG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-A-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---T-----TA-TG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TT-TT-G-T--A-CGG----G--A-A--G---AA-ACCC-T---C-G-A--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-TCG-G-GG-C-T-G-A-C-G-----G-T---A-C-CG--------T-A-A-G---------A-A-----------T--AA-------------GG-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-TT-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--C-T-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-TG------TT-G-A--------------G-G-T-G---GG-C--------------GG--A--ATT-----------T-G-T-C-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TG-----------------------------A-C---A-A--A-G-A-AC-A-CC----AA-T--C--GC-GAA-G--G-C----A-----G--T-T-C-A---CCA-----A--AC-A-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0007145 M01867_242_000000000_ATWYL_1_1108_25380_19971 .CTA--G-A-G--GC-TT--CG-T-C------AA-T-G-C--CC---------------------------GA-A--A--G-G--G-T-G-A-A-C-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-C-G--G--GA--------------------------T-G--A--A--G-G-GC---------TT-CG-------G-C-T--------T-G-T--A---AA-C-C-GC--------------------------TT-TT-G-T--T-TGG----G--A-A--G---AA--TGC-C---C-G-G--A-T----------------------C--G----------------------------T---------------------------------G---A---------------------A---T-CCG-G-GA-G-G-G-A-C-G-----G-T---A-C-CA--------G-A-C-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---GG-AG-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--A-TT-G-G-T----------------------AA----G-T-C-T------------G-G-C--G--TG--A-AA-TC--TCC-AC-C-A-----------------------------------------------------CT-C-AA-----------C-G-G-G-G-A-G--A-G----G-T--C-G---C--T--------------G--GA-A-A---C---------------T--G-C--CG--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--T-----T-GG------CA-G-A--------------G-G-G-T---CG-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-G-A-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----C-AG--GG-G--AG-C-A-AA--CG

Otu0001022 M01867_242_000000000_ATWYL_1_1107_23470_7799 .AGG--G-A-A--TA-TT--GC-A-T------AA-T-G-G--GC---------------------------GA-A--A--G-C--CTG---A-T-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-A--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-T-AC--------------------------TT-TT-G-G--A-GGG----G--A-T--G---A----------------------------------------------G----------------------------GA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-C-CC--------T-T-C-G---------A-A-----------T--AA-------------GC-T--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAA------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TG-T-G-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--G-C--AC--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----T-GA------GA-G-A--------------G-G-G-A---AG-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----G--C-T-T-C---CTG-----G--CT-C-A------------------------------------------------------------------------------T-A------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-G--AG-C-A-AA--CG

Otu0001945 M01867_242_000000000_ATWYL_1_2109_12213_6871 .AGG--G-A-A--TA-TT--GG-TC-------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------C-G-T--A---AA-G-C-AC--------------------------TT-TT-C-T--G-GGA----G--A-T--G---A----------------------------------------------G----------------------------AA--------------------------------C------------------------------------------G-G-A-C-A-----G-T---A-T-CC--------C-A-G-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--T-TC-G-G-C----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-C-AA-----------C-T-A-G-G-A-G--A-G----G-T--C-G---T--T--------------C--GA-T-A---C---------------T--A-C--CG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--G-----C-AA------CA-G-A--------------G-G-A-A---AG-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----A--C-T-T-T---CTG-----G--GT-T-G------------------------------------------------------------------------------C-A------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0003087 M01867_242_000000000_ATWYL_1_1110_8276_9403 .AGG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GA-A--A----G--CCT-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------C-G-T--A---AA-G-C-AC--------------------------TT-TT-C-T--G-GGA----G--A-T--G---A----------------------------------------------G----------------------------AA--------------------------------C------------------------------------------G-G-A-C-A-----G-T---A-T-CC--------C-A-G-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--T-TC-G-G-C----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-C-AA-----------C-T-A-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-A-A---C---------------T--A-C--CG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--G-----C-AT------CA-G-A--------------G-G-A-A---AG-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----A--C-T-T-T---CTG-----G--GA-T-G------------------------------------------------------------------------------C-A------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0008257 M01867_242_000000000_ATWYL_1_2108_21769_26802 .AGG--G-A-A--TA-TT--GG-T-T------AA-T-G-T--GG---------------------------GA-A--A--C-C--G-C-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-T-TC--------------------------TT-TT-C-A--A-TGG----G--A-C--G---A----------------------------------------------G----------------------------GA--------------------------------A------------------------------------------G-G-A-C-G-----G-T---A-C-CA--------T-T-G-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AT-------G-TA-G-G-C-G---------------G--T-CT-T-G-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--G-C--GA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-A--G-----T-GA------TA-G-A--------------G-G-A-A---GA-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----G--T-C-T-T---CTG-----G--AT-C-A------------------------------------------------------------------------------C-T------T-C-T--GA--CG---C----------------------------T-C--A-GA--T-G-CG-A--AA-G-C----T-AA--GG-T--AG-C-A-AA--CG

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Chapter 3

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Otu0000209 M01867_242_000000000_ATWYL_1_1101_7807_9648 .AGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-C-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-GC--------------------------TT-TT-A-G--A-GGA----G--A-T--G---A----------------------------------------------G----------------------------AA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-T-CC--------T-C-T-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-GAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCT-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GT-GT-------G-CA-G-G-C-G---------------G--T-TT-G-G-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-A-A---C---------------T--A-C--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-A--A-----T-GG------GA-G-A--------------G-G-G-A---GA-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-A--G-C----G-----G--T-C-T-C---CTA-----G--AC-C-A------------------------------------------------------------------------------T-T------T-C-T--GA--CG---C----------------------------T-T--A-AA--C-A-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0000872 M01867_242_000000000_ATWYL_1_1107_3533_11400 .AGG--G-A-A--TA-TT--GG---T------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-C-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-GC--------------------------TT-TT-T-A--G-AGG----G--A-C--G---A----------------------------------------------G----------------------------GA--------------------------------A------------------------------------------G-G-A-C-G-----G-T---A-C-CT--------C-T-A-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GAT------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TC-G-G-A----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CC-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A------G-GT-C-G---T--T--------------C--AA-A-A---C---------------T--A-C--CG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----T-GA------GA-G-A--------------G-G-A-A---GA-T--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-A--G-C----G-----A--T-C-T-T---CTG-----G--CT-C-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0001154 M01867_242_000000000_ATWYL_1_1107_22854_13120 .AGG--G-A-A--TA-TT--GG-T--------AA-T-C-T--GC---------------------------GA-A--A--G-C--G-G-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TT-T-G--G-GGG----G--A-T--G---A----------------------------------------------G----------------------------GT--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-C-CT--------C-C-A-G---------A-A-----------T--AA-------------GT-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GAG------------------ACT-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TT-G-G-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--G-T--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-A--A-----T-GG------AA-G-A--------------G-G-A-A---GG-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----A--C-C-T-T---CTG-----G--TC-C-A------------------------------------------------------------------------------T-T------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0001328 M01867_242_000000000_ATWYL_1_1104_3164_18429 .AGG--G-A-A--TA-TT--GGTT--------AA-T-G-T--GC---------------------------GA-A--A--G-C--G-C-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TT-T-G--A-GGG----G--A-T--G---A----------------------------------------------G----------------------------GA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-C-CT--------T-C-A-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-T-G---------------G--T-TC-G-G-A----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CC-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--T-C--TG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----T-GG------GA-G-A--------------G-G-G-A---GG-T--------------GG--A--ATT-----------C-C-G-T-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TA-----------------------------C-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-A--G-C----G-----G--C-C-T-C---CTG-----G--CC-C-A------------------------------------------------------------------------------T-T------T-C-T--GA--CA---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0001400 M01867_242_000000000_ATWYL_1_1103_10262_7942 .AGG--G-A-A--TA-TT--GG-T--------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-AC--------------------------TT-TT-T-G--A-GGG----G--A-T--G---A----------------------------------------------G----------------------------GT--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-C-CT--------T-C-A-G---------A-A-----------T--AA-------------GT-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GAG------------------ACA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--T-CT-G-A-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--G-T--TA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-A--G-----T-GG------GA-G-A--------------G-G-G-A---GG-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----G--C-C-T-C---CTG-----G--CC-C-A------------------------------------------------------------------------------T-T------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0001485 M01867_242_000000000_ATWYL_1_1106_20841_14675 .AGG--G-A-A--TA-TT--GG-G--------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-AC--------------------------TT-TT-A-G--C-AGG----G--A-C--G---A----------------------------------------------G----------------------------GA--------------------------------A------------------------------------------G-G-A-C-G-----G-T---A-C-CT--------G-C-A-G---------A-A-----------T--AA-------------GC-T--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GAA------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--T-CT-G-G-A----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CC-G-G-----------------------------------------------------CT-A-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--A-C--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----T-GA------GA-G-A--------------G-G-G-A---GG-T--------------GG--A--ATT-----------C-C-G-T-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TA-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----A--C-C-T-C---CTG-----G--CT-C-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0002006 M01867_242_000000000_ATWYL_1_1111_21353_21967 .AGG--G-A-A--TA-TT---G-G-T------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-C-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-GC--------------------------TT-TT-T-A--G-AGG----G--A-C--G---A----------------------------------------------G----------------------------GA--------------------------------A------------------------------------------G-G-A-C-G-----G-T---A-C-CT--------C-T-A-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GAT------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TC-G-G-A----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A------G-GT-C-G---T--T--------------C--AA-T-A---C---------------T--A-C--CG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----T-GA------GA-G-A--------------G-G-A-A---GA-T--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-A--G-C----G-----A--T-C-T-T---CTG-----G--CT-C-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0002147 M01867_242_000000000_ATWYL_1_1103_23187_10993 .AGG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GA-A--A----G--CCT-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------C-G-T--A---AA-G-C-AC--------------------------TT-TT-C-T--G-GGA----G--A-T--G---A----------------------------------------------G----------------------------AA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-T-CC--------C-A-G-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TC-G-G-C----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-T-AA-----------C-T-A-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--A-C--CG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--G-----C-AT------CA-G-A--------------G-G-A-A---GG-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----A--C-C-T-T---CTG-----G--GA-T-G------------------------------------------------------------------------------T-T------C-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0002537 M01867_242_000000000_ATWYL_1_1113_9791_26480 .AGG--G-A-A--TA-TT--GG-TC-------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------C-G-T--A---AA-G-C-AC--------------------------TT-TT-C-T--G-GGA----G--A-T--G---A----------------------------------------------G----------------------------AA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-T-CC--------C-A-G-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--T-TC-G-G-C----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-C-AA-----------C-T-A-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-A-A---C---------------T--A-C--CG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-G--G-----C-AT------CA-G-A--------------G-G-A-A---AG-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----A--C-T-T-T---CTG-----G--GA-T-G------------------------------------------------------------------------------C-A------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0003141 M01867_242_000000000_ATWYL_1_1109_24563_20126 .AGG--G-A-A--TA-TT--G--G-T------AA-T-G-G--GC---------------------------GA-A--A--G-C--CTG---A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TT-T-A--G-AGG----G--A-C--G---A----------------------------------------------G----------------------------GA--------------------------------A------------------------------------------G-G-A-C-G-----G-T---A-C-CT--------T-T-A-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GAT------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TC-G-G-A----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CC-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-A-A---C---------------T--A-C--CG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----T-GA------GA-G-A--------------G-G-A-A---GA-T--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----A--T-C-T-T---CTG-----G--CT-C-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----C-AG--GG-T--AG-C-A-AA--CG

Otu0003178 M01867_242_000000000_ATWYL_1_1102_23973_21921 .AGG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------C-G-T--A---AA-G-C-AC--------------------------TT-TT-C-T--G-GGA----G--A-T--G---A----------------------------------------------G----------------------------AA--------------------------------C------------------------------------------G-G-A-C-A-----G-T---A-T-CC--------C-A-G-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--T-TC-G-G-C----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-C-AA-----------C-T-A-G-G-A-G--A-G----G-T--C-G---T--T--------------C--GA-T-A---C---------------T--A-C--CG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--G-----C-AT------CA-G-A--------------G-G-A-A---AG-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----A--C-T-T-T---CTG-----G--GA-T-G------------------------------------------------------------------------------C-A------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0003216 M01867_242_000000000_ATWYL_1_1102_25649_10418 .AGG--G-A-A--TA-TT--GG-T--------AA-T-G-T--GC---------------------------GA-A--A--G-C--G-C-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-C-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-GC--------------------------TT-TT-T-A--T-GGG----G--A-T--G---A----------------------------------------------G----------------------------GA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-C-TC--------A-T-A-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GT-------G-TA-G-G-C-G---------------G--T-TT-G-A-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-A-A---C---------------T--A-T--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-A--G-----T-GG------TA-G-A--------------G-G-G-A---GG-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----G--C-C-T-C---CTG-----G--CC-C-A------------------------------------------------------------------------------T-T------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-A-CG-A--AA-G-C----T-AA--GG-T--AG-C-A-AA--CG

Otu0003489 M01867_242_000000000_ATWYL_1_2104_10799_4194 .AGG--G-A-A--TA-TT---G-G-T------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TT-T-A--G-AGG----G--A-C--G---A----------------------------------------------G----------------------------GA--------------------------------A------------------------------------------G-G-A-C-G-----G-T---A-C-CT--------C-T-A-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GAT------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TC-G-G-A----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CC-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A------G-GT-C-G---T--T--------------C--AA-A-A---C---------------T--A-C--CG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----T-GA------GA-G-A--------------G-G-A-A---GA-T--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-A--G-C----G-----A--T-C-T-T---CTG-----G--CT-C-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0003605 M01867_242_000000000_ATWYL_1_1101_16473_20786 .AGG--G-A-A--TA-TT--GG-T-T------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-C-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-C-A--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-T-GC--------------------------TT-TT-T-G--G-AGG----G--A-T--G---A----------------------------------------------G----------------------------GA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-C-CT--------T-C-A-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GT-------G-CA-G-G-C-G---------------G--T-TT-G-G-T----------------------TA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--G-C--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--A-----T-GG------GA-G-A--------------G-G-A-A---GG-T--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----G--C-C-T-T---CTG-----G--CC-C-A------------------------------------------------------------------------------T-C------T-C-T--GA--CG---C----------------------------T-C--A-TA--C-A-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0004794 M01867_242_000000000_ATWYL_1_1105_10883_15206 .AGG--G-A-A--TA-TT--GC-T-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-A-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-C-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-GC--------------------------TT-TT-C-T--G-GGA----G--A-T--G---A----------------------------------------------G----------------------------AA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-T-CT--------C-A-G-G---------A-A-----------T--AA-------------GT-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GAG------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TC-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--T-TC-G-G-T----------------------AA----G-T-T-G------------G-G-C--G--TG--A-AA-TC--T-C-CT-G-G-----------------------------------------------------CT-T-AA-----------C-T-A-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--A-C--CG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-A--G-----T-GG------TA-G-A--------------G-G-A-A---GA-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----A--T-C-T-T---CTG-----G--AC-C-A------------------------------------------------------------------------------T-T------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-T-A-AA--CG

Otu0005012 M01867_242_000000000_ATWYL_1_1103_9423_21695 .AGG--G-A-A--TA-TT--GG-TC-------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------C-G-T--A---AA-G-C-AC--------------------------TT-TT-C-T--G-AGA----G--A-T--G---A----------------------------------------------G----------------------------AA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-T-CT--------C-A-G-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--T-TC-G-G-C----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-T-AA-----------C-T-A-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--A-C--CG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--G-----C-AT------CA-G-A--------------G-G-A-A---GG-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----A--C-C-T-T---CTG-----G--GA-T-G------------------------------------------------------------------------------T-T------C-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0005357 M01867_242_000000000_ATWYL_1_1107_19490_20012 .AGG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GA-A--A--GC---C-T-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-C-A--T--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------C-G-T--A---AA-G-T-GC--------------------------TT-TTCT-G--A-GA-----G--A-T--G---A----------------------------------------------G----------------------------AA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A---TC--------T-C-AGG---------A-A-----------T--AA-------------GT-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-T-G---------------G--T-TT-G-G-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--G-C--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-A--G-----C-GG------TA-G-A--------------G-G-G-A---GG-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----G--C-C-T-C---CTG-----G--AC-C-G------------------------------------------------------------------------------T-T------T-C-T--GA--CA---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0005372 M01867_242_000000000_ATWYL_1_1101_24045_25079 .AGG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GA-A--A--GC---C-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-C-A--C--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------C-G-T--A---AA-G-T-GC--------------------------TT-TTCT-G--A-GA-----G--A-T--G---A----------------------------------------------G----------------------------AA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A---TC--------T-C-AGG---------A-A-----------T--AA-------------GT-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GAG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-T-G---------------G--T-TC-G-G-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--A-C--TG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-A--G-----C-AG------TA-G-A--------------G-G-G-A---GG-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----G--C-C-T-C---CTG-----G--AC-T-G------------------------------------------------------------------------------T-T------T-C-T--GA--CA---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0006615 M01867_242_000000000_ATWYL_1_2110_14041_7743 .AGG--G-A-A--TA-TT--GG-T--------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TT-T-G--A-GGG----G--A-T--G---A----------------------------------------------G----------------------------GT--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-C-CC--------T-C-A-G---------A-A-----------T--AA-------------GT-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GAG------------------ACA-A-G------------C--G--T---T--AT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--T-CT-G-G-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--G-T--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-A--G-----T-GG------GA-G-A--------------G-G-G-A---GG-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----G--C-C-T-C---CTG-----G--CC-C-A------------------------------------------------------------------------------T-T------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA—CG

Otu0008128 M01867_242_000000000_ATWYL_1_1105_13688_19937 .AGG--G-A-A--TA-TT--GG---G------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-AC--------------------------TT-TT-A-G--C-AGG----G--A-C--G---A----------------------------------------------G----------------------------GA--------------------------------A------------------------------------------G-G-A-C-G-----G-T---A-C-CT--------G-C-A-G---------A-A-----------T--AA-------------GC-T--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GAA------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--T-CT-G-G-A----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CC-G-G-----------------------------------------------------CT-A-AA-----------C-T-G-G-G-A-G--A------G-GT-C-G---T--T--------------C--AA-T-A---C---------------T--A-C--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--A-----T-GA------GA-G-A--------------G-G-G-A---GG-T--------------GG--A--ATT-----------C-C-G-T-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TA-----------------------------C-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-A--G-C----G-----A--C-C-T-C---CTG-----G--CT-C-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0008871 M01867_242_000000000_ATWYL_1_1102_26786_16485 .AGG--G-A-A--TA-TT--GG-T-T------AA-T-G-T--GC---------------------------GA-A--A--G-C--G-C-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-C-G--C--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-GC--------------------------TT-TT-T-G--A-AGG----G--A-T--G---A----------------------------------------------G----------------------------GA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-C-CT--------T-C-A-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--T-TC-A-G-A----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--T-C--TG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----T-GG------GA-G-A--------------G-G-G-A---GG-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----G--C-C-T-C---CTG-----G--CC-C-A------------------------------------------------------------------------------T-T------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0010112 M01867_242_000000000_ATWYL_1_1113_10470_24173 .AGG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GA-A--A--GC---C-T-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-C-A--T--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------C-G-T--A---AA-G-T-GC--------------------------TT-TTCT-G--A-GA-----G--A-T--G---A----------------------------------------------G----------------------------AA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A---TC--------T-C-AGG---------A-A-----------T--AA-------------GT-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-T-G---------------G--T-TT-G-G-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--G-C--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-A--A-----C-GG------TA-G-A--------------G-G-G-A---AG-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----G--C-T-T-C---CTG-----G--AC-C-G------------------------------------------------------------------------------T-T------T-C-T--GA--CA---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0011635 M01867_242_000000000_ATWYL_1_2112_21022_9384 .CGG--G-A-A--TA-TT--GC-A-C------AA-T-G-A--GC---------------------------GA-A--A--G-C--T-T-G-A-T-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-C-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-GC--------------------------TT-TT-C-G--G-GGG----G--A-T--G---A----------------------------------------------G----------------------------GA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-C-CC--------C-C-G-G---------A-A-----------T--AA-------------GT-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GTG------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TT-G-G-G----------------------TA----G-T-C-G------------G-G-C--A--TG--A-AA-TC--T-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-T-T-G-G-A-G--A-G----G-C--T-G---T--C--------------C--GA-T-A---C---------------T--C-C--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--G-----C-AA------CA-G-A--------------G-G-G-C---GA-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--A--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--T-C-G-C---CTG-----G--GT-T-G------------------------------------------------------------------------------C-A------C-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AG-G-C----T-AG--GG-G--AG-A-G-AA--CG

Otu0000094 M01867_242_000000000_ATWYL_1_1101_21653_8540 .GGG--G-A-A--TT-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-A-----A--T-ACC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--A--T-G-CC---------TA-TG-------G-G-T--------T-G-T--A---AA-C-C-TC--------------------------TT-TT-T-T--C-AGG----G--A-G--G---A-------------------------------------------A--C----------------------------AA--------------------------------A---A--------------------------------------T-G-A-C-GT----G-T---A-C-CT--------G-A-A-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---GG-AG-GAT------------------GCA-A-G------------T--G--T---T--AT-C-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-CT-------G-TA-G-G-T-G---------------G--T-TT-A-A-T----------------------AA----G-T-C-A------------A-C-T--G--TT--A-AA-TC--T-C-GA-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-C-G-A-A--A-T----C-G--C-A---G--T--------------C--GA-A-A---C---------------T--A-T--TA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-AG------TA-G-G--------------G-G-T-A---AA-G--------------GG--A--ATT-----------T-C-C-A-GT--GG-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TT-----------------------------G-G---A-A--A-G-A-AC-A-CC----GA-T--G--GC-GAA-G--G-C----A-----C--T-T-T-A---CTG-----G--GC-T-A------------------------------------------------------------------------------T-T------A-C-T--AA--CA---C----------------------------T-G--A-GA--G-A-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--TG

Otu0000433 M01867_242_000000000_ATWYL_1_1101_6314_23380 .GGG--G-A-A--TT-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-G--G--GA--------------------------T-G--A--A--G-G-CC---------TC-TG-------G-G-C--------T-G-T--A---AA-C-C-TC--------------------------TT-TT-C-T--C-AAG----G--A-A--G---A-------------------------------------------A--G----------------------------AC--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-TT--------G-A-G-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------T--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-GA-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GT-CC-------G-CA-G-G-C-G---------------G--C-CC-T-G-T----------------------AA----G-T-C-T------------G-T-C--G--TT--A-AA-GC--G-T-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-A-T-T--T-C----A-G--C-G---A--T--------------G--GA-A-A---C---------------T--A-C--AG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----T-GG------TA-G-G--------------G-G-C-A---GA-G--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----C--T-C-T-G---CTG-----G--GC-C-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-C--A-TG--G-A-CG-A--AA-G-C----C-AG--GG-G--AG-C-G-AA--AG

Otu0000036 M01867_242_000000000_ATWYL_1_1101_17771_2473 .GGG--G-A-A--TT-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-A-----A--G-ACC-G-C-G-T-----G-G-G--G--GA--------------------------G-G--A--A--G-G-CC---------CT-TG-------G-G-T--------T-G-T--A---AA-C-C-CC--------------------------TT-TT-A-T--C-AGG----G--A-A--GA--A-------------------------------------------A--A----------------------------AA--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------G-A-T-G---------A-A-----------T--CA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GT-CC-------G-CA-G-G-T-G---------------G--C-CG-A-G-C----------------------AA----G-T-C-C------------G-C-G--G--TT--A-AA-GA--C-C-GG-A-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-G-G-A--A-G----A-G--C-C---G--T--------------A--GA-A-A---C---------------T--G-A--AA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-A--C-----C-GG------TA-G-G--------------G-G-T-A---GC-G--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-TC----GG-T--G--GC-GAA-A--G-C----G-----C--G-C-T-A---CTG-----G--GC-C-G------------------------------------------------------------------------------G-A------A-C-T--GA--CA---C----------------------------T-C--A-GG--G-A-CG-A--AA-G-C----T-AG--GG-G--AG-C-G-AA--AG

Otu0001299 M01867_242_000000000_ATWYL_1_1101_14177_7669 .GGG--G-A-A--TT-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-A-----A--T-ACC-G-C-G-T-----G-A-G--G--GA--------------------------G-G--A--A--G-G-CT---------CT-TG-------G-G-T--------C-G-T--A---AA-C-C-TC--------------------------TT-TT-C-T--C-AAG----G-----------A-------------------------------------------A--G---------------------------ATA--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-TT--------G-A-G-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TG-A--T-T--GG-GC-------------------GT--A-----AA-GA-GT-CC-------G-TA-G-G-T-G---------------G--T-AA-T-T-C----------------------AA----G-T-C-T------------G-T-G--G--TT--A-AA-GC--G-T-GG-A-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-A-T-A--A-G----G-G--C-C---A--T--------------G--GA-A-A---C---------------T--G-A--AT--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----A-GG------TA-G-G--------------G-G-T-A---GA-G--------------GG--A--ATT-----------C-T-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-A--G-C----G-----C--T-C-T-A---CTG-----G--AC-C-T------------------------------------------------------------------------------T-A------A-C-T--GA--CA---C----------------------------T-G--A-GG--G-A-CG-A--AA-G-C----T-AG--GG-G--AG-C-G-AA--AG

Otu0000318 M01867_242_000000000_ATWYL_1_2108_7302_18033 .AGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--C--G-G-TT---------TT-CG-------G-A-T--------T-G-T--A---AA-C-C-TC--------------------------TT-TT-A-T--C-TGG----G--A-A--G---AA--A--------------A------A-----------------T--T--------------------------------------------------------------T--------A--------------------A--A--A----T-T-G-A-C-G-----G-T---A-C-CA--------G-A-T-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GTG------------------GCG-A-G------------C--G--T---T--AC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-AC-------G-TA-G-G-C-G---------------G--T-TT-T-T-T----------------------AA----G-T-C-T------------G-G-A--G--TG--A-AA-TG--T-C-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-C-G-G-A--T-C----T-G--C-T---T--T--------------G--GA-T-A---C---------------T--G-G--AA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--G-----T-TG------GA-G-A--------------G-G-A-A---GA-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC---AGA----G--GC-GAA-G--G-C----G-----G--T-C-T-T---CTG-----G--CC-A-A------------------------------------------------------------------------------A-C------A-T-T--GA--CG---C----------------------------T-G--A-GG--T-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001405 M01867_242_000000000_ATWYL_1_1106_21203_24792 .AGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--C--G-G-TT---------TT-CG-------G-A-T--------T-G-T--A---AA-C-C-TC--------------------------TT-TT-A-T--C-TGG----G--A-A--G---AA--A--------------A--------------------------------------------------------AA--------------------------------T-------------------------C---A--A--A--A-T-T-G-A-C-G-----G-T---A-C-CA--------G-A-T-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GTG------------------GCG-A-G------------C--G--T---T--AC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-AC-------G-TA-G-G-C-G---------------G--T-TT-T-T-T----------------------AA----G-T-C-T------------G-A-A--G--TG--A-AA-TG--T-C-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-C-G-G-A--T-C----T-G--C-T---T--T--------------G--GA-T-A---C---------------T--G-G--AA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--G-----T-TG------GA-G-A--------------G-G-A-A---GA-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--T-C-T-T---CTG-----G--CC-A-A------------------------------------------------------------------------------A-C------A-T-T--GA--CG---C----------------------------T-G--A-GG--T-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002297 M01867_242_000000000_ATWYL_1_1102_19998_18201 .AGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-T-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-T--G--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-C-A-TC--------------------------TT-TT-A-T--A-GGG----G--A-A--G---AA--TGA-T---T-A-TC-G----T-G-----------------C--T----------------------------AA--------------------------------T--------C---------------AG---C-GAT-A-AA-G-T-G-A-C-G-----G-T---A-C-CT--------T-A-T-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GTG------------------GCG-A-G------------C--G--T---T--AC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GA-GT-AC-------G-TA-G-G-C-G---------------G--A-TA-T-A-T----------------------AA----G-T-C-A------------G-G-A--G--TG--A-AA-TT--T-T-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-C-G-A-A--A-C----T-G--C-T---T--T--------------T--GA-A-A---C---------------T--G-T--GT--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GT------GA-G-G--------------G-G-A-T---GA-T--------------GG--A--ATT-----------T-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC---GAT----G--GC-GAA-G--G-C----A-----G--T-C-A-T---CTG-----G--CA-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--T-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0005991 M01867_242_000000000_ATWYL_1_1106_23114_19000 .AGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GG---------------------------GC-A--A--C-C--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-A-C-G-T-----G-T-G--G--GA--------------------------T-G--A--A--G-C-TT---------TT-CG-------G-A-G--------T-G-T--A---AA-C-C-AC--------------------------TG-TC-G-T--C-CGT----G--A-A--C---AA-GGCG-------G-T------------------------------C----------------------------GC--------------------------------A-------------------------------GGA-C-CG-T-T-G-A-G-G-----G-T---A-A-CG--------G-G-A-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-AAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-GG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA--G-GGAGC-------G-CA-G-G-C-G---------------G--G-TT-G-G-C----------------------GT----G-T-C-A------------G-G-T--G--TC--T-AA-TT--C-C-GG-G-G-----------------------------------------------------CC-T-AA-----------C-C-C-C-G-G-G--T-C----G-G--C-G---C--T--------------T--GA-A-A---C---------------T--G-C--CA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--C-----G-GT------CA-G-A--------------G-G-T-A---GG-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AG-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGA----G--GC-GAA-G--G-C----G-----G--C-C-T-A---CTG-----G--GA-C-C------------------------------------------------------------------------------A-G------A-T-T--GA--CG---C----------------------------T-G--A-GG--C-T-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002337 M01867_242_000000000_ATWYL_1_2106_21505_6891 .AGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GG---------------------------GG-A--A--C-C--C-T-G-A-T-G-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-TT---------TT-CG-------G-A-T--------T-G-T--A---AA-C-T-TC--------------------------TA-TC-G-A--T-GGG----G--A-A--G---A-------------------------------------------A--A----------------------------AA--------------------------------A---A--------------------------------------T-G-A-C-G-----G-T---A-C-CC--------A-A-G-T---------A-A-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AT-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-TA-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-AC--C-C-AT-G-G-----------------------------------------------------CT-T-AA-----------C-T-A-T-G-G-G--A-A----T-G--C-A---T--T--------------T--GA-A-A---C---------------T--A-T--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-AG------GA-G-A--------------G-G-T-A---AG-T--------------GG--A--ATT-----------T-C-T-A-GT--GT-A-G-CG-GT--T--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--A-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----A--C-T-T-A---CTG-----G--AC-T-G------------------------------------------------------------------------------A-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0000072 M01867_242_000000000_ATWYL_1_1110_9300_13728 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--G--GA--------------------------A-G--A--A--G-G-CT---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TG-A-T--T-AGG----G--A-C--G---A-------------------------------------------A--T----------------------------AA--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------A-A-A-G---------A-A-----------C--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GT-------G-TA-G-G-C-G---------------G--G-AC-A-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TT--C-C-GA-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-C-G-G-C--G-C----T-G--C-A---T--C--------------T--GA-A-A---C---------------T--A-T--AG--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----A-TG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-A-T------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000512 M01867_242_000000000_ATWYL_1_2105_27945_17411 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--G--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TG-A-T--T-AGG----G--A-C--G---A-------------------------------------------A-------------------------------TA--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------A-A-G-G---------A-A-----------C--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GT-------G-TA-G-G-C-G---------------G--G-AC-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TT--C-C-GA-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-C-G-G-C--G-C----T-G--C-A---T--C--------------T--GA-A-A---C---------------T--A-T--AG--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----A-TG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-A-T------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0007275 M01867_242_000000000_ATWYL_1_1103_21663_21079 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--G--GA--------------------------A-G--A--A--G-G-CT---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TG-A-T--C-AGG----G--A-C--G---A-------------------------------------------A-------------------------------TA--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------G-A-G-G---------A-A-----------C--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCG-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GT-------G-TA-G-G-C-G---------------G--G-AA-C-G-C----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TT--C-C-AG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-T-G-G-C--G-C----T-G--C-A---T--C--------------T--GA-A-A---C---------------T--G-T--GT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-TG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-A-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0009375 M01867_242_000000000_ATWYL_1_2111_14221_22758 .GGG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-CT---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TG-A-T--C-AGG----G--A-C--G---A-------------------------------------------A--A----------------------------AA--------------------------------A---A--------------------------------------T-G-A-C-G-----G-T---A-C-CT--------G-A-A-A---------A-A-----------C--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GT-------G-TA-G-G-C-G---------------G--G-TC-T-G-C----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TT--C-C-CG-G-G-----------------------------------------------------CT-T-AA-----------C-T-C-G-G-G-C--G-G----G-T--C-A---T--C--------------C--GA-A-A---C---------------T--G-C--GG--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-TG------GA-G-A--------------G-G-A-T---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--A--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-T---CTG-----G--AC-A-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0027005 M04041_72_000000000_AUMPE_1_2105_7120_14336 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--G--GA--------------------------A-G--A--A--G-G-CT---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TG-A-T--T-AGG----G--A-C--G---A-------------------------------------------A--T----------------------------AA--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------A-A-A-G---------A-A-----------C--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GT-------G-TA-G-G-C-G---------------G--G-AC-A-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TT--C-C-GA-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-C-G-G-C--G-C----T-G--C-A---T--C--------------T--GA-A-A---C---------------T--A-T--AG--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----A-TG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-A-T------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----T-AG--GG-G--AG-C-G-AA--AG

Otu0015348 M01867_242_000000000_ATWYL_1_2112_29282_14170 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TT-C-T--A-GGG----G--A-C--G---AA-GGAC-T---A-C-G--G-G--A-G-----------------T--A----------------------------AT--------------------------------A----------------------T-GT---C-CGT-A-GA-G-T-G-A-C-G-----G-T---A-C-CT--------T-A-G-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---GT-AG-GGG------------------GCG-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GA-GC-AC-------G-TA-G-G-T-G---------------G--A-CC-T-A-T----------------------AA----G-T-C-A------------G-G-T--G--TG--A-AA-GA--C-C-GG-G-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-G-G-G--G-T----T-G--C-A---C--T--------------T--GA-A-A---C---------------T--G-T--TG--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--G-----C-AG------GA-G-A--------------G-G-A-A---AG-T--------------AG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AA-A-TC-----------------------------A-G---G-A--G-G-A-AT-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-T-G------------------------------------------------------------------------------A-C------C-C-T--GA--CA---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0003707 M01867_242_000000000_ATWYL_1_1111_7347_21776 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CT---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-C-T--A-GGG----G--A-A--G---A-------------------------------------------A--TA---------------------------AA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CC--------T-G-G-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-T-G---------------G--T-TC-G-A-C----------------------AA----G-T-C-A------------G-A-A--G--TG--A-AA-GG--C-T-AC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-T-G-G-T--A------A-G--C-T---T--T--------------T--GA-A-A---C---------------T--G-T--TG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-AG------GA-G-A--------------G-G-T-A---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----A--C-T-T-A---CTG-----G--AC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CA---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004472 M01867_242_000000000_ATWYL_1_2111_20434_11342 .GGG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--G--GA--------------------------A-G--A--A--G-G-CT---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-TC--------------------------TG-TC-C-T--T-GGT----G--A-C--GA--A-------------------------------------------A--A----------------------------AA------------------------------------A--------------------------------------T-G-A-C-G-----G-T---A-G-CC--------A-A-G-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGG------------------GCG-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-T-G---------------G--C-CT-T-T-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-TA--C-C-CG-G-G-----------------------------------------------------CT-T-AA-----------C-C-T-G-G-G-G--G-G----T-G--C-A---T--C--------------T--AA-T-A---C---------------T--G-G--AA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-AG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CA---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0013817 M01867_242_000000000_ATWYL_1_1114_17637_28213 .GAA--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--G--GA--------------------------A-G--A--A--G-G-TT---------CT-CG-------G-A-T--------T-G-T--A---AA-C-C-TC--------------------------TG-TC-C-C--A-TGG----G--A-A--G---A-------------------------------------------A--A----------------------------AA--------------------------------A---A--------------------------------------T-G-A-C-G-----G-T---A-C-CA--------T-G-G-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--C-CC-A-T-C----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-TC--C-C-CG-T-G-----------------------------------------------------CT-T-AA-----------C-A-T-G-G-G-A--A-T----T-G--C-A---T--T--------------C--AA-A-A---C---------------T--G-A--AG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----T-GG------AA-G-A--------------G-G-T-A---AG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----A--C-T-T-A---CTG-----G--GC-C-A------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004716 M01867_242_000000000_ATWYL_1_1101_26680_11274 .GGG--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------CT-TG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-A-T--C-GGG----G--A-C--G---AA-CGGC-T---T-T-A--T-C--A-------------------G--GA---------------------------A---------------------------------A---T--------------------GG---G-TAG-A-GA-T-T-G-A-C-G-----G-T---A-C-CC--------G-A-G-A---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCG-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GC-------G-CA-G-G-T-G---------------G--G-AT-C-T-T----------------------AA----G-T-C-T------------G-T-T--C--TG--A-AA-GT--T-C-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-G-T-G--A------T-G--G-G---A--T--------------G--GA-A-A---C---------------T--G-G--GA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-CG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATT-----------C-C-C-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GCG-AA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-G-A------------------------------------------------------------------------------T-G------T-C-T--GA--CA---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----C-AG--GG-G--AG-C-G-AA--CG

Otu0008929 M01867_242_000000000_ATWYL_1_2110_12330_16647 .GGG--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------CT-TG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-A-T--C-GGG----G--A-C--G---AA-CGGC-C---A-G-G--T-C--A-------------------G--GA---------------------------A---------------------------------A---T--------------------GG---G-CCT-G-GA-T-T-G-A-C-G-----G-T---A-C-CC--------G-A-G-A---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCG-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GC-------G-CA-G-G-T-G---------------G--G-AT-C-T-T----------------------AT----G-T-C-T------------G-C-T--T--TA--A-AA-GT--G-C-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-G-T-G--A------T-G--G-G---G--T--------------G--GA-A-A---C---------------T--G-A--GA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-CG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATT-----------C-C-C-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GCG-AA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-G-A------------------------------------------------------------------------------T-G------T-C-T--GA--CA---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----C-AG--GG-G--AG-C-G-AA--CG

Otu0014118 M01867_242_000000000_ATWYL_1_2113_29353_14310 .GGG--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------CT-TG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-A-T--T-GGG----G--A-C--G---AA-CGAG-C---A-C-G--T-C--A-------------------G--GA---------------------------A---------------------------------A---T--------------------GG---G-CGT-G-AA-G-T-G-A-C-G-----G-T---A-C-CC--------A-A-G-A---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GC-------G-CA-G-G-T-G---------------G--G-AT-C-T-T----------------------AA----G-T-C-C------------A-T-T--T--TG--A-AA-GT--T-C-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-G-T-G--A------T-G--G-G---A--T--------------G--GA-A-A---C---------------T--G-G--GA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-CG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATT-----------C-C-C-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GCG-AA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-G-A------------------------------------------------------------------------------T-G------T-C-T--GA--CA---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----C-AG--GG-G--AG-C-G-AA--CG

Otu0001327 M01867_242_000000000_ATWYL_1_2103_16282_26475 .GAG--G-A-A--TA-TT--GG-G-C------AA-T-G-C--CC---------------------------GC-A--A--G-G--G-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--A--T-G-CC---------GT-AA-------G-G-T--------T-G-T--A---AA-C-T-CC--------------------------TT-TT-G-A--A-GGG----G--A-C--G---AA--AAA-G---C-A-G--A-T-------------------------A----------------------------GT----------------------------------------------------------C---T-C-T-G-CA-T-T-G-A-C-T-----G-T---A-C-CC--------T-T-A-G---------A-A-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-A-GGGG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--G-TT-T-G-T----------------------AA----G-T-C-A------------G-A-G--G--TG--A-AA-TC--C-T-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-C----T-G--C-C---T--C--------------T--GA-T-A---C---------------T--G-C--AA--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-AG------AA-G-A--------------G-A-G-A---AG-C--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-TA-GT--G--------A-A-A---TA-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-C---TTG-----G--TC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001421 M01867_242_000000000_ATWYL_1_1106_22882_9978 .GAG--G-A-A--TA-TT--GG-G-C------AA-T-G-C--CC---------------------------GC-A--A--G-G--G-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--A--T-G-CC---------GT-AA-------G-G-T--------T-G-T--A---AA-C-T-CC--------------------------TT-TT-T-G--A-GGG----G--A-C--G---AA--AAA-A---C-A-G--A-T-------------------------A----------------------------GT----------------------------------------------------------C---T-C-T-G-TA-T-T-G-A-C-T-----G-T---A-C-CT--------T-C-A-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-A-GGGG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--G-TT-T-G-T----------------------TA----G-T-C-A------------G-A-G--G--TG--A-AA-TC--C-T-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-C----T-G--C-C---T--C--------------T--GA-T-A---C---------------T--G-C--AA--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-AG------AA-G-A--------------G-A-G-A---AG-C--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-TG-GT--G--------A-A-A---TA-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-C---TTG-----G--TC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0005927 M01867_242_000000000_ATWYL_1_1111_11992_22546 .GAG--G-A-A--TA-TT--GG-G-C------AA-T-G-C--CC---------------------------GC-A--A--G-G--G-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--A--G-G-TC---------GT-AA-------G-A-T--------C-G-T--A---AA-C-T-CC--------------------------TT-TT-G-A--A-GGG----G--A-C--G---AA-AAAA-T---G-G-G--T-T-------------------------A----------------------------AC----------------------------------------------------------A---G-CCC-A-TA-T-T-G-A-C-T-----G-T---A-C-CC--------T-T-A-G---------A-A-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-A-GGGG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--A-TT-T-A-T----------------------AA----G-T-C-G------------A-T-G--G--TG--A-AA-TC--C-T-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-C----T-G--C-C---G--T--------------C--GA-T-A---C---------------T--G-C--AA--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----T-GG------AA-G-A--------------G-A-G-A---AG-C--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-TA-GT--G--------A-A-A---TA-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-C---TTG-----G--TC-C-A------------------------------------------------------------------------------T-A------T-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0007864 M01867_242_000000000_ATWYL_1_1108_6104_7710 .GAG--G-A-A--TA-TT--GG-G-C------AA-T-G-C--CC---------------------------GC-A--A--G-G--G-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--A--G-G-TC---------GT-AA-------G-A-T--------C-G-T--A---AA-C-T-CC--------------------------TT-TT-G-A--A-AGG----G--A-A--G---AA-AAAA-T---G-G-A--G-T-------------------------A----------------------------AC----------------------------------------------------------A---C-TCT-A-TA-T-T-G-A-C-T-----G-T---A-C-CT--------T-T-A-G---------A-A-----------A--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-A-GGGG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--G-TT-T-G-T----------------------TA----G-T-C-G------------A-T-G--G--TG--A-AA-TC--C-T-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-A-G-A--A-C----T-G--C-C---G--T--------------C--GA-T-A---C---------------T--G-C--AA--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-GG------AA-G-A--------------G-A-G-A---AG-C--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-TA-GT--G--------A-A-A---TA-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-C---TTG-----G--TC-C-G------------------------------------------------------------------------------T-A------T-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001781 M01867_242_000000000_ATWYL_1_1109_18240_9939 .CGA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-T--T--GA--------------------------T-G--A--A--G-C-TT---------TT-CG-------G-A-G--------T-G-T--A---AA-G-A-TC--------------------------TT-TT-A-T--G-AGG----G--A-A--G---AA------------------------------------------G--T----------------------------TT------------------------------------A------------------------------------T-T-G-A-C-G-----G-T---A-C-CT--------C-A-T-G---------A-A-----------T--AA-------------GG-G--GC-T---C-C-TAA-----------------------T--C-T-C--G-T---------G-C-CA--G-C---A--G-G--AG---C-----------------GG--TA-AT-------AC---GA-GA-GCC------------------CCG-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-T-G---------------G--T-TG-T-G-T----------------------TA----G-T-C-G------------T-T-T--G--TC--A-AA-TC--T-C-CG-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-G-G-A-A--T-C----C-G--C-G---A--A--------------C--GA-A-A---C---------------G--G-C--AC--A-AC--------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-A--G-----T-GT------GA-G-G--------------G-G-T-A---TA-G--------------GG--A--ACT-----------A-A-T-A-GT--GT-A-G-GG-GT--G--------A-A-A---TC-C-GT-TG--AT-A-TT-----------------------------A-T---T-G--G-G-A-AC-A-CC----AA-A--A--GC-GAA-G--G-C----A-----C--T-A-T-A---CTG-----G--CA-C-A------------------------------------------------------------------------------T-A------T-C-T--GA--CA---C----------------------------T-C--A-AG--C-A-CG-A--AA-G-C----G-TG--GG-T--AG-C-G-AA--TG

Otu0002202 M01867_242_000000000_ATWYL_1_1102_13210_13516 .CGA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-T--T--GA--------------------------T-G--A--A--G-C-CT---------TT-CG-------G-G-G--------T-G-T--A---AA-A-A-TC--------------------------TT-TT-A-T--G-AGG----G--A-T--G---AC---------------------------------------------A----------------------------T-------------------------------------A------------------------------------G-C-G-A-C-A-----G-T---A-C-CT--------C-A-T-G---------A-A-----------T--AA-------------GG-G--GC-T---G-C-TAA-----------------------T--C-T-C--G-T---------G-C-CA--G-C---A--G-C--AG---C-----------------GG--TA-AT-------AC---GA-GA-GCC------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GA-GT-TT-------G-TA-G-G-C-G---------------G--C-GT-A-G-T----------------------TA----G-T-C-T------------T-C-T--G--TT--A-AA-TC--C-A-TC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-A-T-G-A--A-C----C-A--C-A---G--A--------------G--GA-A-A---C---------------G--G-C--TA--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----T-GT------GA-G-A--------------G-G-T-G---AA-T--------------GG--A--ACT-----------C-A-T-A-GT--GT-A-G-GG-GT--G--------A-A-A---TC-C-GT-TG--AT-A-TT-----------------------------A-T---G-G--G-G-A-AC-A-CC----AA-A--A--GC-GAA-G--G-C----A-----G--T-T-C-A---CTG-----G--CA-C-A------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-GA--A-A-CG-A--AA-G-C----G-TG--GG-T--AG-C-G-AA--AG

Otu0004551 M01867_242_000000000_ATWYL_1_1105_25198_16124 .CGA--G-A-A--TA-TT--CG-A-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-C-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-T-GC---------TT-CG-------G-T-A--------T-G-T--A---AA-C-T-GC--------------------------TT-TT-G-T--G-AAC----C--A-G--A---AA---------------------------------------------GT---------------------------TT------------------------------------A------------------------------------T-T-G-A-T-C-----G-----G-T-TC----------A-A-G---------A-A-----------T--AA-------------GA-G--GT-T---G-C-TAA-----------------------A--C-T-C--G-T---------G-C-CA--G-C---A--G-C--AG---C-----------------GG--TA-AT-------AC---GA-GT-GCC------------------TCG-A-G------------C--G--T---T--AT-C-CGG-AA------TC-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GT-------G-CA-G-G-C-G---------------G--T-CT-G-A-T----------------------TA----G-T-C-T------------T-C-T--G--TT--A-AA-TT--T-C-TC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-G-G-A-A--C-C----T-G--C-A---G--G--------------G--GA-A-A---C---------------G--G-T--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--A-----T-GC------GA-G-A--------------G-G-T-T---AA-C--------------GG--A--ACT-----------C-A-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-TG--AT-A-TT-----------------------------A-T---G-G--G-G-A-AC-A-CC----AA-A--T--GC-GAA-G--G-C----A-----G--T-T-A-A---CTG-----G--AG-C-A------------------------------------------------------------------------------C-T------C-C-T--GA--CG---C----------------------------T-G--A-AA--C-A-CG-A--AA-G-C----G-TG--GG-T--AG-C-G-AA--TG

Otu0005795 M01867_242_000000000_ATWYL_1_1102_11717_2839 .CAA--G-A-A--TC-TT--CC-T-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-A-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-T-A--T--GA--------------------------A-G--A--A--G-C-CC---------TT-CG-------G-G-G--------T-G-T--A---AA-A-T-AC--------------------------TT-TT-A-T--C-TGG----G--A-C--G---A----------------------------------------------A---------------------------CAA------------------------------------A--------------------------------------T-G-A-C-G-----G-T---A-C-CA--------G-A-T-G---------A-A-----------TA-A--------------GG-G--GC-T---G-C-TAA-----------------------C--C-T-C--G-T---------G-C-CA--G-C---A--G-C--AG---C-----------------GG--TA-AT-------AC---GA-GG-GCC------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GT-CC-------G-TA-G-G-T-G---------------G--T-TT-A-T-C----------------------GC----A-T-C-T------------C-C-T--G--TT--A-AA-TC--T-C-AA-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-T-G-A-G--T-C----A-G--C-G---G--G--------------A--GA-G-A---T---------------G--G-A--CA--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-A--C-----T-GG------AA-G-A--------------G-G-C-A---AA-C--------------GG--A--ATT-----------G-C-T-G-GT--GT-A-G-GG-GT--T--------A-A-A---TC-C-AT-TA--AT-A-TC-----------------------------A-G---C-A--A-G-A-AC-A-CC----AA-A--T--GC-GAA-G--G-C----A-----G--T-T-T-G---CTA-----G--GA-C-A------------------------------------------------------------------------------G-T------T-C-T--GA--CA---CT---------------------------C----AGTG--G-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--TG

Otu0008698 M01867_242_000000000_ATWYL_1_1111_19794_23368 .CGA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-T--T--GA--------------------------A-G--A--A--G-C-CT---------TT-CG-------G-G-G--------C-G-T--A---AA-A-A-TC--------------------------TT-TT-A-T--G-AGG----G--A-T--G---AC---------------------------------------------A----------------------------T-------------------------------------A------------------------------------G-C-G-A-C-A-----G-T---A-C-CT--------C-A-T-G---------A-A-----------T--AA-------------GG-G--GC-T---G-C-TAA-----------------------T--C-T-C--G-T---------G-C-CA--G-C---A--G-C--AG---C-----------------GG--TA-AT-------AC---GA-GA-GCC------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GA-GT-TT-------G-TA-G-G-C-G---------------G--T-AC-T-G-T----------------------TA----G-T-C-T------------T-C-T--G--TT--A-AA-TC--T-C-TC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-A-G-A-A--A-C----C-A--C-A---G--A--------------G--GA-A-A---C---------------G--G-C--AG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-GT------GA-G-A--------------G-G-T-G---AA-T--------------GG--A--ACT-----------C-A-T-A-GT--GT-A-G-GG-GT--G--------A-A-A---TC-C-GT-TG--AT-A-TT-----------------------------A-T---G-G--G-G-A-AC-A-CC----AA-A--A--GC-GAA-G--G-C----A-----G--T-T-C-A---CTG-----G--CA-C-A------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-GA--A-A-CG-A--AA-G-C----G-TG--GG-T--AG-C-G-AA--AG

Otu0003283 M01867_242_000000000_ATWYL_1_1102_22883_9743 .CGA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-C-G--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TG-TC-A-G--A-TGG----G--AGG--A---AATTC------------------------------------------ATGGGGGT--------------------TCTCCCC---------------------------------------------------------------------TGT-GATTGAC-----TGA---A-C-GT--------C-A---G---------A-G-----------G--AA-------------GC-A--CA-G---G-C-TAA-----------------------G--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GA-AC-TGT------------------GCA-A-A------------C--G--T---T--AT-T-CGG-AA------TT-A--C-T--GG-GC-------------------TT--A-----AA-GA-GT-TC-------G-TA-G-G-C-G---------------G--C-CC-T-A-T----------------------AA----G-T-C-G------------G-G-T--G--TG--A-AA-TC--C-C-CC-A-G-----------------------------------------------------CT-C-AA-----------C-T-G-G-G-G-A--A-C----T-G--C-G---C--T--------------C--GA-A-A---C---------------T--G-C--AG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--G-----A-GA------TA-G-A--------------G-G-T-G---AG-C--------------GG--A--ACT-----------G-A-T-G-GT--GG-A-G-CG-GT--G--------A-A-A---TG-C-GT-TG--AT-A-CT-----------------------------A-T---C-A--G-G-A-AC-A-CC----GG-T--T--GC-GAA-A--G-C----G-----G--C-T-C-A---CTG-----G--GT-C-T------------------------------------------------------------------------------C-T------T-C-T--GA--CG---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----T-AG--GG-G--AG-C-A-AA--CG

Otu0000498 M01867_242_000000000_ATWYL_1_1113_11460_23942 .GGG--G-A-A--TC-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-C-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-GC--------------------------TT-TT-G-T--C-AGG----G--A-G--C---AA--ATC-T---T-T-G--A-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CC---T-CCG-A-GG-A-T-G-A-G-A-----G-T---A-C-CT--------G-A-A-G---------A-A-----------T--AA-------------GG-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------CCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-T-G---------------G--C-CC-G-T-T----------------------AA----G-A-G-T------------G-A-T--G--TG--A-AA-TC--C-C-CG-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-G-G-G-A--A-T----T-G--C-A---T--T--------------G--CT-A-A---C---------------T--G-G--CG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC---GAT----G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--CC-T-G------------------------------------------------------------------------------C-T------A-C-T--GA--CA---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0003387 M01867_242_000000000_ATWYL_1_2109_27020_18712 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GG---------------------------GC-A--A--C-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--C-CGG----G--A-A--A---AA--ATC-G---C-A-C--G-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------TC---T-GTG-T-GG-A-T-G-A-T-G-----G-T---A-C-CG--------G-A-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TT-T-T-T----------------------AA----G-A-C-A------------G-G-C--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-G---C--T--------------T--GT-G-A---C---------------T--G-G--AA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-GG------CA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC----AA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GT-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004577 M01867_242_000000000_ATWYL_1_2101_7746_11155 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CT---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-T-A--G-AGG----G--A-A--G---AA-ATGA-T---A-T-T--T-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CC---A-GAT-T-TC-T-T-G-A-C-G-----G-T---A-C-CT--------C-T-G-A---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TT-G-T-T----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-GC--C-C-TG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-G----T-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-G--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-GA-GT--G--------A-A-A---TC-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-T-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0008472 M01867_242_000000000_ATWYL_1_1105_9801_11603 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CT---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-T-A--G-AGG----G--A-A--G---AA-ATGA-T---T-T-T--G-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CC---T-GAT-T-TC-T-T-G-A-C-G-----G-T---A-C-CT--------C-T-G-A---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TT-G-T-T----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-GC--C-C-TG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-G----T-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-G--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-GA-GT--G--------A-A-A---TC-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-T-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0009817 M01867_242_000000000_ATWYL_1_2111_10040_13193 .GGG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-C-C-A---GC-C-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TG-G-G--G-AGG----G--A-C--G---AA-TCGG-T---A-C-G--G-G----------------------C--T----------------------------AA--------------------------------C---A----T----------------C---C-CGT-G-CT-T-T-G-A-C-G-----G-T---A-C-CT--------T-C-T-T---------A-G-----------C--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-A------------C--G--T---T--GC-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-TA-G-G-C-G---------------G--T-CC-G-T-T----------------------AT----G-T-C-G------------G-G-T--G--TG--A-AA-GC--C-C-TC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-A-G-G-A--A-G----T-G--C-A---T--T--------------C--GA-A-A---C---------------T--G-G--CG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-TG------GA-G-A--------------G-G-G-T---CG-C--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-G-A-C---CTG-----G--AC-A-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002097 M01867_242_000000000_ATWYL_1_1101_12330_4112 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TG-G-G--G-AGG----A--A-A--G---AA-TAAG-G---G-C-A--G-G----------------------C--T----------------------------AA--------------------------------T---C----A----------------C---T-GC--C-TG-A-T-G-A-C-G-----G-T---A-C-CT--------C-C-T-T---------A-G-----------C--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---AG-AG-GGT------------------GCA-A-A------------C--G--T---T--GT-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--C-TT-T-G-C----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TC--C-C-AG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-T-G-G-A--A-G----T-G--C-A---C--C--------------C--GA-A-A---C---------------T--G-C--AG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-T--C-----T-CG------GA-G-A--------------G-G-A-A---GG-T--------------GG--A--ACG-----------C-T-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-A---G-C--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-C-T-T---CTG-----G--AC-G-A------------------------------------------------------------------------------T-G------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

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Chapter 3

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Otu0002423 M01867_242_000000000_ATWYL_1_2106_13130_26487 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TG-G-G--G-AGG----G--A-A--G---AA-TAAG-G---G-C-A--G-G----------------------T--T----------------------------AA--------------------------------T---A----T----------------C---C-TGT-C-TG-A-T-G-A-C-G-----G-T---A-C-CT--------C-C-T-T---------A-G-----------C--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---AG-AG-GGT------------------GCA-A-A------------C--G--T---T--GT-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-T---------------G--C-CT-T-G-A----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TC--C-C-TC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-A-G-G-A--A-G----T-G--C-A---C--C--------------C--GA-A-A---C---------------T--A-C--AA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--C-----C-CG------GA-G-A--------------G-G-A-A---GG-T--------------GG--A--ATG-----------C-T-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-A---G-C--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-C-T-T---CTG-----G--AC-G-G------------------------------------------------------------------------------T-G------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002817 M01867_242_000000000_ATWYL_1_2109_16857_19203 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TG-G-G--G-AGA----G--A-A--G---AA-TAAG-G---C-G-G--T-G----------------------C--T----------------------------AA------------------------------------T----C----------------A---G-CCG-C-TG-A-T-G-A-C-G-----G-T---A-T-CT--------C-C-T-T---------A-G-----------C--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---AG-AG-GGT------------------GCA-A-A------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-T-G---------------G--C-TT-C-G-C----------------------AA----G-T-C-G------------A-A-T--G--TG--A-AA-AC--C-C-CA-G-G-----------------------------------------------------CT-C-AC-----------C-C-T-G-G-G-A--A-G----A-G--C-A---T--C--------------C--GA-A-A---C---------------T--G-C--GT--C-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----C-CG------GA-G-A--------------G-G-A-A---GG-T--------------GG--A--ATG-----------C-T-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-A---G-C--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-C-T-T---CTG-----G--AC-G-G------------------------------------------------------------------------------T-G------A-C-T--GA--CA---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0003609 M01867_242_000000000_ATWYL_1_1104_20480_16709 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TG-G-G--G-AGG----G--A-C--G---AA-TAAG-G---C-C-A--G-G----------------------C--T----------------------------AA--------------------------------T---C----A----------------C---T--GG-T-TG-A-T-G-A-C-G-----G-T---A-C-CT--------C-C-T-T---------A-G-----------C--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---AG-AG-GGT------------------GCA-A-A------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-CA-T-G-C----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TC--C-C-AC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-T-G-G-T--A-G----T-G--C-A---C--C--------------C--GA-A-A---C---------------T--A-C--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-T--C-----C-TG------GA-G-A--------------G-G-A-A---GG-T--------------GG--A--ATG-----------C-T-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AG-A-TC-----------------------------A-A---G-C--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-C-T-T---CTG-----G--AC-A-G------------------------------------------------------------------------------T-G------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0009301 M01867_242_000000000_ATWYL_1_1113_10996_3637 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------CT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TG-G-G--G-AGA----G--A-C--G---AA-TAAG-G---C-T-C--A-G----------------------T--T----------------------------AA--------------------------------T---A----G----------------C---T-GTG-C-CG-A-T-G-A-C-G-----G-T---A-T-CT--------C-C-T-T---------A-G-----------C--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---AG-AG-GGT------------------GCA-A-A------------C--G--T---T--GT-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TT-T-T-C----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-AC--C-C-AG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-T-G-G-A--A-G----T-G--C-A---C--C--------------C--GA-A-A---C---------------T--G-G--AA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----C-TG------GA-G-A--------------G-G-A-A---GG-T--------------GG--A--ATG-----------C-T-C-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------G-A---G-C--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-C-T-T---CTG-----G--AC-A-G------------------------------------------------------------------------------T-G------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0016167 M01867_242_000000000_ATWYL_1_2101_24746_5280 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-CC--------------------------TG-TG-G-G--G-AGG----G--A-C--G---AA-TAAG-T---G-T-C--G-G----------------------T--C----------------------------AA--------------------------------T---A----C----------------C---C-GGC-A-TG-A-T-G-A-C-G-----G-T---A-C-CT--------C-C-T-T---------A-G-----------C--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---AG-AG-GGT------------------GCA-A-A------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--C-CG-C-G-T----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-GC--C-C-TC-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-G-G-G-A--A-G----T-G--C-A---C--T--------------C--GA-A-A---C---------------T--G-C--GC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--C-----C-CG------GA-G-A--------------G-G-A-T---CG-T--------------GG--A--ATT-----------C-T-C-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------G-A---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-G-A-T---CTG-----G--AC-G-G------------------------------------------------------------------------------T-G------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002828 M01867_242_000000000_ATWYL_1_1105_9701_3678 .GGG--G-A-A--TC-TT--GC-A-C------AA-T-G-G--GG---------------------------GC-A--A--C-C--C-T-G-A-T-G-C-A---GC-G-----A--T-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TT-G-A--G-AGT----G--A-A--G---AA-TAAG-T---G-T-A--T-T----------------------C--G----------------------------AA--------------------------------C---A----G----------------G---G-TAC-A-TG-A-T-G-A-C-A-----C-T---A-G-CT--------C-T-T-T---------A-G-----------A--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---AG-AG-GGT------------------GCA-A-A------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TA-C-C-T----------------------AA----G-T-C-G------------G-G-T--G--TG--A-AA-GT--T-T-GC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-T-A-A-A--A-G----A-G--C-A---T--T--------------C--GA-A-A---C---------------T--G-G--GC--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-TG------GA-G-A--------------G-G-A-A---GA-C--------------GG--A--ATA-----------C-A-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-T---G-T--A-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--T-C-T-T---CTG-----G--AC-A-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002051 M01867_242_000000000_ATWYL_1_1102_11871_9332 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------CT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TG-G-G--G-AGA----G--A-A--G---AA-TAAG-A---G-T-A--G-G----------------------C--T----------------------------AA--------------------------------T---C----A----------------C---T-AC--T-TG-A-T-G-A-C-G-----G-T---A-T-CT--------C-C-T-T---------A-G-----------C--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GGT------------------GCA-A-A------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--C-TT-C-G-C----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-GT--C-C-AT-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-T-G-G-A--A-G----T-G--C-A---C--C--------------C--GA-A-A---C---------------T--G-C--GT--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-T--T-----C-TG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATG-----------C-T-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-A---G-C--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-A-G------------------------------------------------------------------------------T-G------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004900 M01867_242_000000000_ATWYL_1_1104_8595_23718 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------CT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TG-G-G--G-AGG----G--A-C--G---AA-TAAG-G---T-G-A--A-G----------------------C--T----------------------------AA--------------------------------C---A----C----------------C---T-TCA-C-TG-A-T-G-A-C-G-----G-T---A-C-CT--------C-T-T-T---------A-G-----------C--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---AG-AG-GGT------------------GCA-A-A------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-CT-C-G-C----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TC--C-C-AG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-T-G-G-A--A-G----T-G--C-A---C--C--------------C--GA-A-A---C---------------T--G-C--AA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--C-----C-TG------GA-G-A--------------G-G-A-A---GG-T--------------GG--A--ATG-----------C-T-C-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------G-A---G-C--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-C-T-T---CTG-----G--AC-A-G------------------------------------------------------------------------------T-G------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0005849 M01867_242_000000000_ATWYL_1_1102_18913_17238 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------CT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TG-G-G--G-AGG----G--A-C--G---AA-TAAG-G---T-G-A--A-G----------------------C--T----------------------------AA--------------------------------C---A----C----------------C---T-TCA-C-TG-A-T-G-A-C-G-----G-T---A-C-CT--------C-T-T-T---------A-G-----------C--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---AG-AG-GGT------------------GCA-A-A------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-T-G---------------G--T-CT-C-G-C----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TC--C-C-AG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-T-G-G-A--A-G----T-G--C-A---C--C--------------C--GA-A-A---C---------------T--G-C--AA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--C-----C-TG------GA-G-A--------------G-G-A-A---GG-T--------------GG--A--ATG-----------C-T-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-A---G-C--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-C-T-T---CTG-----G--AC-A-G------------------------------------------------------------------------------T-G------A-C-T--GA--CA---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0011616 M01867_242_000000000_ATWYL_1_2109_15884_12554 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-CC--------------------------TG-TG-G-G--A-GGG----G--A-C--G---AA-TAAG-G---G-T-T--G-G----------------------A--G----------------------------AA--------------------------------A---T----G----------------C---C-AAT-C-TG-A-T-G-A-C-G-----G-T---A-C-CC--------T-T-T-T---------A-G-----------C--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---AG-AG-GGT------------------GCA-A-A------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-T---------------G--C-TG-C-G-T----------------------GT----G-T-C-G------------G-A-T--G--TG--A-AA-GC--C-C-TC-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-G-G-G-A--A-G----T-G--C-A---C--T--------------C--GA-A-A---C---------------T--A-C--GC--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----T-CG------GA-G-A--------------G-G-G-T---CG-T--------------GG--A--ATT-----------C-T-C-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------G-A---G-A--G-G-A-AC-A-CC----TG-T--G--GC-GAA-G--G-C----G-----G--C-G-A-C---CTG-----G--AC-G-A------------------------------------------------------------------------------A-G------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0019478 M01867_242_000000000_ATWYL_1_1107_27099_14177 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------CT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TG-G-G--G-AGA----G--A-A--G---AA-TAAG-A---G-T-A--G-G----------------------C--T----------------------------AA--------------------------------T--------C----------------A---C-TAC-T-TG-A-T-G-A-C-G-----G-T---A-T-CT--------C-C-T-T---------A-G-----------C--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GGT------------------GCA-A-A------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--C-TT-C-G-C----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-GT--C-C-AT-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-T-G-G-A--A-G----T-G--C-G---C--C--------------C--GA-A-A---C---------------T--G----CGT-A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-T--T-----C-TG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATG-----------C-T-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-A---G-C--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G----CTT-T---CTG-----G--AC-A-G------------------------------------------------------------------------------T-G------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0008641 M01867_242_000000000_ATWYL_1_2114_21236_20914 .GGG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-C-C-A---GC-G-----A--C-GCC-G-T-G-T-----G-G-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-CC--------------------------TG-TG-A-G--G-TGG----G--A-A--G---AA-GGGC-T---C-A-G--A-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CC---T-TTG-G-GCTT-T-G-A-C-G-----G-T---A-C-CA--------C-C-A-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-T-G---------------G--T-TG-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-T-TA-G-G-----------------------------------------------------CT-T-AA-----------C-C-T-G-A-G-A--A-C----T-G--C-A---T--C--------------T--GA-T-A---C---------------T--G-C--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-T--A-----C-AG------CA-G-A--------------G-G-G-A---AA-C--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--T-T-T-C---CTG-----G--GC-T-G------------------------------------------------------------------------------A-T------A-C-T--GA--CA---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002177 M01867_242_000000000_ATWYL_1_1101_16163_17034 .......................................................................TA-G--G--G-A--A-T-T-T-T-G-C-G---CA-A-----T--G-GGC-G-A-A-A-----G-C-C--T--GA--------------------------C-G--C--A----G--C---------AA-CG-------C-C-G--------C-G-T--G---AA---C-GA--------------------------TG-AC-G-C--C-TTT----C--G-G--G---GT-GTAA-A---G-T-T--C-T--G-------------------T--C----------------------------AG--------------------------------T---G----G---------------GG---A-CGA-A-AT-T-T-G-A-C-G-----G-T---A-C-CC--------A-C-A-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TC-A--T-T--GG-GC-------------------GT--A-----AA-GA-GT-TC-------G-TA-G-G-T-G---------------G--T-AT-G-T-T----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-GC--C-C-GA-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-C-G-G-A--T-C----G-G--C-A---C--T--------------G--GA-T-A---C---------------T--G-G--CA--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--G-----T-GG------TA-G-A--------------G-G-T-A---AA-G--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-TC----GG-T--G--GC-GAA-A--G-C----G-----C--T-T-T-A---CTG-----G--GC-C-A------------------------------------------------------------------------------T-T------A-T-T--GA--CA---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--TG

Otu0004082 M01867_242_000000000_ATWYL_1_1109_14247_22972 .AGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------T-T--A--A--G-C-TT---------TT-CG-------G-A-G--------T-G-T--A---AA-G-T-TC--------------------------TG-TC-A-G--T-GGG----G--A-C--G----A--A-A-------C-------------------------------------------------------------T----------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CC--------A-C-A-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TC-A--T-T--GG-GC-------------------GT--A-----AA-GA-GT-TC-------G-TA-G-G-T-G---------------G--T-AT-G-T-T----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-GG--C-A-GA-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-C-T-G-T--A-A----G-G--C-A---C--T--------------G--GA-T-A---C---------------T--G-G--CA--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--G-----T-GG------TA-G-A--------------G-G-T-A---AA-G--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-TC---GGT----G--GC-GAA-A--G-C----G-----C--T-T-T-A---CTG-----G--GC-C-A------------------------------------------------------------------------------T-T------A-T-T--GA--CA---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----C-GG--GG-T--AG-C-A-AA--TG

Otu0010548 M01867_242_000000000_ATWYL_1_1103_10751_23753 .......................................................................TA-G--G--G-A--A-T-T-T-T-G-C-G---CA-A-----T--G-GGC-G-A-A-A-----G-C-C--T--GA--------------------------C-G--C--A--G-C-AA---------CG-CC-------G-C-G--------T-G-A--A---TG-A-T-TA--------------------------AG-CC-C-C--T-CGG----G--G-T--G---TA-AAGT-----T-C-T----G----------------------T--C----------------------------AG--------------------------------T---G----G---------------GG---A-CGA-A-AC-T-T-G-A-C-G-----G-T---A-C-CC--------A-C-A-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TC-A--T-T--GG-GC-------------------GT--A-----AA-GA-GT-TC-------G-TA-G-G-T-G---------------G--T-AT-G-T-T----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-GC--C-C-GA-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-C-G-G-T--T-C----G-G--C-A---C--T--------------G--GA-T-A---C---------------T--G-G--CA--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--G-----T-GG------TA-G-A--------------G-G-T-A---AA-G--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-TC----GG-T--G--GC-GTA-A--G-C----G-----C--T-T-T-A---CTG-----G--GC-C-A------------------------------------------------------------------------------T-A------A-T-T--GA--CA---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----C-GG--GG-T--AG-C-A-AA--TG

Otu0000567 M01867_242_000000000_ATWYL_1_1112_13329_5236 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--AG---------------------------GA-A--A--C-T--C-T-G-A-T-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--C--G-C-AT---------TT-CG-------G-T-G--------T-G-T--A---AA-C-T-CC--------------------------TT-TT-A-T--A-AGG----G--A-A--G---A----------------------------------------------T----------------------------AA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------T-A-T-G---------A-A-----------T--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--AC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-AT-GT-------G-TA-G-G-C-T---------------G--T-AC-T-A-T----------------------AA----G-T-C-A------------G-G-A--G--TG--A-AA-TC--C-A-AC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-T-T-G-A--A-C----T-G--C-T---C--T--------------T--GA-A-A---C---------------T--G-T--TG--T-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--A-----T-GG------GA-G-A--------------G-G-T-A---GA-T--------------GG--A--ATT-----------G-G-T-G-GT--GT-A-G-GG-GT--A--------A-A-A---TC-C-GT-AG--AT-A-TC-----------------------------A-C---C-A--G-G-A-AT-A-CC----GA-T--T--GC-GAA-G--G-C----G-----A--T-C-T-A---CTG-----G--AA-C-A------------------------------------------------------------------------------T-T------A-T-T--GA--CG---C----------------------------T-G--A-GA--C-A-TG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000447 M01867_242_000000000_ATWYL_1_1101_17612_10281 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-A-----A--T-ACC-G-C-G-T-----G-T-G--T--GA--------------------------A-G--A--A--G-G-CC---------TG-AG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TC-A-A--T-GGG----G--A-G--G---AA--AAC-C---T-G-C--C-G--G-------------------T--C----------------------------AA--------------------------------T---A----C---------------CC---G-GCA-G-C--T-T-G-A-C-A-----T-T---A-C-CT--------T-T-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-TA-G-G-C-G---------------G--T-TG-A-T-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-T-AA-----------C-C-T-G-G-G-A--A-C----G-G--C-A---T--T--------------T--GA-T-A---C---------------T--G-G--TT--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----G-AG------TA-G-A--------------G-G-G-G---AG-T--------------GG--A--ATT-----------T-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-G---A-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----A--C-T-C-C---CTG-----G--AC-T-C------------------------------------------------------------------------------A-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--T-A-CG-A--AA-G-C----G-TG--GG-T--AG-C-A-AA--CA

Otu0001893 M01867_242_000000000_ATWYL_1_1112_17983_16468 .TAG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TA-G-C--C-GAC----G--A-A--T---A----------------------------------------------T----------------------------AA---------------------------------------------------------------------------T-G-A-C-T-----G-T---A-G-TC--------G-G-A-A---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-GG-GGG------------------GCG-A-G------------C--G--T---T--AT-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-TA-G-G-C-G---------------G--T-TA-T-G-A----------------------AA----G-T-T-A------------G-G-A--G--TG--A-AA-GC--C-C-AG-G-G-----------------------------------------------------CT-T-AA-----------C-C-T-T-G-G-A--A-T----A-G--C-T---T--T--------------T--AA-A-A---C---------------T--A-C--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--T-----C-GA------TA-G-A--------------G-G-A-T---GG-C--------------GG--A--ATT-----------A-T-C-A-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------G-A---T-A--G-G-A-AC-A-CC----GG-T--G--GC-GTA-A--G-C----G-----G--C-C-A-T---CTA-----G--AT-C-G------------------------------------------------------------------------------A-C------A-T-T--GA--CG---C----------------------------T-G--A-GG--T-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0008418 M01867_242_000000000_ATWYL_1_1112_21148_13033 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-T-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-CC--------------------------TG-TC-G-G--G-AGG------------G----A------------C--------------------------------G-----------------------------A--------------------------------A-----------------------------------T-AC---T-G-A-C-G-----G-T---A-C-CT--------C-C-C-A---------A-G-----------A--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GC-T-CGA-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--A-AT-G-G-T----------------------AG----G-T-C-G------------G-T-T--G--TG--A-AA-GC--C-C-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-C-G-G-A--A-C----T-G--C-A---T--T--------------C--GA-A-A---C---------------C--A-C--CA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-A---AG-C--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----G--C-T-T-C---CTG-----G--CC-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0009443 M01867_242_000000000_ATWYL_1_1113_17332_16717 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-C-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-CC--------------------------TG-TC-G-G--GAGGGAA--GA-AAGCCTGCAACCGC---------------------------------------A--G-G--------------------------TC---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------C-G-C-A---------A-G-----------A--AA-------------GC-G--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGC------------------GCA-A-G------------C--G--T---T--GC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-CT-G-A-T----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TC--C-C-CA-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------C--GA-A-A---C---------------T--G-C--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-A---AG-C--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-C---CTG-----G--CT-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000393 M01867_242_000000000_ATWYL_1_2102_12025_10645 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-A-T--A-GGC----G--A-G--G---AA-TAAG-A---T-C-C--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-GGG-T-CG-A-T-G-A-C-G-----G-T---A-G-CC--------T-A-T-GA----------A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-C-G---------------G--T-TC-T-A-C----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-TC--C-C-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-G-G-A--C-C----G-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-T--GG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-TG------GA-G-G--------------G-G-T-A---GC-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC---AAT----G--GC-GAA-G--G-C----A-----A--G-C-T-A---CTG-----G--CC-A-T------------------------------------------------------------------------------G-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001208 M01867_242_000000000_ATWYL_1_2106_12689_17723 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-G-----A--T-GCC-G-C-G-T-----G-A-T--C--GA--------------------------T-G--A--A--G-G-TC---------GA-GA-------G-A-T--------T-G-T--A---AA-G-A-TC--------------------------TT-TT-A-C--A-GCT----G--A-G--G---AA-TAAG-T---C-T-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CGG-A-TG-A-T-G-A-C-G-----T-T---A-A-GC--------T-G-T-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AA-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AC-------G-CA-G-G-C-G---------------G--C-CC-G-A-C----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-GA--C-C-TG-G-G-----------------------------------------------------CT-T-AA-----------C-T-C-A-G-G-G--A-C----C-G--C-G---T--T--------------G--GA-A-A---C---------------T--G-T--CG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--C-----T-TG------GA-G-G--------------G-G-G-A---GT-C--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-TG--AT-A-TT-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--A-C-T-C---CTG-----G--CC-G-A------------------------------------------------------------------------------A-G------A-T-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-C----A-TA--GG-G--AG-C-A-AA--CA

Otu0001809 M01867_242_000000000_ATWYL_1_1111_14821_18778 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-A-T--A-GGC----G--A-G--G---AA-TAAG-A---C-T-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CGG-T-CG-A-T-G-A-C-G-----G-T---A-G-CT--------T-A-T-GA----------A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-C-G---------------G--T-TC-A-A-C----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-TC--C-C-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-G-G-A--C-C----G-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-T--TG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-TG------GA-G-G--------------G-G-T-A---GC-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC---AAT----G--GC-GAA-G--G-C----A-----A--G-C-T-A---CTG-----G--CC-A-T------------------------------------------------------------------------------G-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004046 M01867_242_000000000_ATWYL_1_1106_28931_14708 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-G-T--A-GAC----G--A-G--G---AA-TAAG-A---T-C-C--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-GGG-T-CG-A-T-G-A-C-G-----G-T---A-G-TC--------T-G-C-GA----------A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-C-G---------------G--T-TC-T-A-C----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-TC--C-C-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-G-G-A--C-A----G-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-T--GG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-TG------GA-G-G--------------G-G-T-A---GC-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC---AAT----G--GC-GAA-G--G-C----A-----A--G-C-T-A---CTG-----G--CC-A-T------------------------------------------------------------------------------G-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0005835 M01867_242_000000000_ATWYL_1_1111_19716_17535 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-G-T--A-GAC----G--A-G--G---AA-TAAG-A---T-C-C--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-GGG-T-CG-A-T-G-A-C-G-----G-T---A-G-TC--------T-G-C-GA----------A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-C-G---------------G--T-TC-T-A-C----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-GC--C-C-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-G-G-A--A-A----G-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-T--GG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-TG------GA-G-G--------------G-G-T-A---GC-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC---AAT----G--GC-GAA-G--G-C----A-----A--G-C-T-A---CTG-----G--CC-A-T------------------------------------------------------------------------------G-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0009381 M01867_242_000000000_ATWYL_1_1111_8984_14509 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-A-T--A-GGC----G--A-G--G---AA-TAAG-A---T-C-C--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---T-GGG-T-CG-A-T-G-A-C-G-----G-T---A-G-CC--------T-A-T-GA----------A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-C-G---------------G--T-TC-T-A-C----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-TC--C-C-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-G-G-A--C-C----G-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-T--GG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-TG------GA-G-G--------------G-G-T-A---GC-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC---AAT----G--GC-GAA-G--G-C----A-----A--G-C-T-A---CTG-----G--CC-A-T------------------------------------------------------------------------------G-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002565 M01867_242_000000000_ATWYL_1_1106_21231_24505 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--T--GA--------------------------G-G--A--A--G-G-CC---------GA-AA-------G-G-T--------T-G-T--A---AA-A-T-CC--------------------------TT-TT-G-T--C-GAC----G--A-A--G---AA-TAAG-G---G-T-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CGC-C-TG-A-T-G-A-C-G-----T-T---A-G-TC--------G-G-C-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AA-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-CC-T-G-T----------------------AA----G-C-C-C------------G-G-C--G--TG--A-AA-TG--C-T-GG-A-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-A-G-A--G-C----T-G--C-G---C--T--------------G--GG-A-A---C---------------T--G-C--TG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----A-TG------GA-A-G--------------G-G-G-A---GT-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----A--A-C-T-C---CTG-----G--CC-A-A------------------------------------------------------------------------------T-G------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-T----G-CG--GG-G--AG-C-A-AA--CA

Otu0010694 M01867_242_000000000_ATWYL_1_2112_10022_7342 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--T--GA--------------------------C-G--A--A--G-G-CC---------GG-AA-------G-G-T--------T-G-T--A---AA-A-T-CC--------------------------TT-TT-G-C--T-GAG----G--A-A--G---AA-TAAG-G---C-T-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CGG-T-CG-A-T-G-A-C-A-----T-G---A-A-TC--------G-G-C-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AT-------G-TA-G-G-C-G---------------G--T-TT-T-G-T----------------------AA----G-C-C-C------------G-G-C--G--TG--A-AA-TA--T-C-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-G-G-A--G-A----C-G--C-G---C--T--------------G--GG-A-A---C---------------T--G-C--AA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--C-----A-CG------GA-G-G--------------G-G-G-A---GT-T--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AT-A-CC----GG-T--G--GC-GAA-G--G-C----G-----A--A-C-T-C---CTG-----G--CC-G-A------------------------------------------------------------------------------T-G------A-T-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0012123 M01867_242_000000000_ATWYL_1_2110_8660_26237 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--T--GA--------------------------C-G--A--A--G-G-CC---------GA-AA-------G-G-T--------T-G-T--A---AA-A-T-CC--------------------------TT-TT-G-C--T-GGT----G--A-A--G---AA-TAAG-T---C-T-G--G-G--A-------------------G--G----------------------------AA--------------------------------A--------T---------------GC---C-CGG-A-TG-A-T-G-A-C-A-----T-G---A-A-CC--------G-G-T-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-AAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AT-------G-TA-G-G-C-G---------------G--T-CC-T-G-T----------------------AA----G-C-C-T------------G-G-C--G--TT--A-AA-TG--G-T-TC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-A-A-T-C--C-T------G--C-G---T--C--------------G--GG-A-A---C---------------T--G-C--AA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--C-----A-CA------GA-G-G--------------G-G-A-A---AT-C--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AT-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--A-T-T-T---CTG-----G--CT-G-A------------------------------------------------------------------------------T-G------A-T-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000818 M01867_242_000000000_ATWYL_1_1103_27280_18117 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-A-T--A-GGC----G--A-G--G---AA-TAAG-A---T-C-C--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-GGG-T-CG-A-T-G-A-C-G-----G-T---A-G-CC--------T-G-T-GA----------A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-C-G---------------G--T-TC-G-A-A----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-GC--C-C-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-G-G-A--A-A----G-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-T--CG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-TG------GA-G-G--------------G-G-T-A---GC-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC---AAT----G--GC-GAA-G--G-C----A-----A--G-C-T-A---CTG-----G--CC-A-T------------------------------------------------------------------------------G-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-T----A-TG--GG-G--AG-C-A-AA--CA

Otu0001442 M01867_242_000000000_ATWYL_1_1105_5519_24465 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-A-T--G-GAC----G--A-G--G---AA-TAAG-A---C-T-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CGG-T-TG-A-T-G-A-C-G-----G-T---A-G-TC--------C-A-T-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AT-------G-CA-G-G-C-G---------------G--C-TT-T-G-T----------------------AA----G-T-C-T------------G-G-T--G--TG--T-AA-GA--C-C-AC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-G-G-G-G--A-G----C-G--C-A---C--T--------------G--GA-A-A---C---------------T--G-C--GA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-AG------GA-A-G--------------G-G-G-A---GT-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC----AG-T--G--GC-GAA-G--G-C----G-----A--A-C-T-C---CTG-----G--CC-A-T------------------------------------------------------------------------------G-T------A-C-T--GA--CG---C----------------------------T-G--A-GA--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0006080 M01867_242_000000000_ATWYL_1_1113_17368_8895 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-A-T--A-GGC----G--A-G--G---AA-TAAG-A---T-C-C--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-GGG-T-CG-A-T-G-A-C-G-----G-T---A-G-CC--------T-G-T-GA----------A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-C-G---------------G--T-TC-T-A-C----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-TC--C-C-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-G-G-A--C-A----G-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-T--AG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-TG------GA-G-G--------------G-G-T-A---GC-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC---AAT----G--GC-GAA-G--G-C----A-----A--G-C-T-A---CTG-----G--CC-A-T------------------------------------------------------------------------------G-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-T----A-TG--GG-G--AG-C-A-AA--CA

Otu0007269 M01867_242_000000000_ATWYL_1_1103_15520_22090 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GTC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-G-T--G-GAC----G--A-G--G---AA-TAAG-G---C-C-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CGG-C-TG-A-T-G-A-C-G-----G-T---A-G-TC--------C-A-A-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AT-------G-CA-G-G-C-G---------------G--C-TT-T-G-T----------------------AA----G-T-C-T------------G-G-T--G--TG--C-AA-GA--C-C-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-G-G--A-A----C-G--C-A---C--T--------------G--GA-A-A---C---------------T--G-C--GA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-CG------GA-A-G--------------G-G-G-A---GT-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC----AG-T--G--GC-GAA-G--G-C----G-----A--A-C-T-C---CTG-----G--CC-A-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GA--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0007422 M01867_242_000000000_ATWYL_1_1110_15651_5480 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--T--GA--------------------------A-G--A--A--G-G-CC---------GA-AA-------G-G-T--------T-G-T--A---AA-A-T-CC--------------------------TT-TT-G-C--T-GAG----G--A-A--G---AA-TAAG-C---G-T-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CGC-G-TG-A-T-G-A-C-A-----T-G---A-A-TC--------G-G-C-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-AAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AT-------G-TA-G-G-C-G---------------G--T-TC-C-G-T----------------------AA----G-C-C-T------------G-G-C--G--TG--A-AA-TG--G-T-TC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G---A-A-T--C-C----T-G--C-G---T--C--------------G--GG-A-A---C---------------T--G-C--GG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--C-----A-CT------GA-G-G--------------G-G-G-A---AT-C--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AT-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--A-T-T-C---CTG-----G--CA-G-A------------------------------------------------------------------------------T-G------A-T-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0010126 M01867_242_000000000_ATWYL_1_1109_27058_16189 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-A-T--A-GGC----G--A-G--G---AA-TAAG-A---C-T-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CGG-T-CG-A-T-G-A-C-G-----G-T---A-G-CT--------T-A-T-GA----------A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-C-G---------------G--T-TC-A-A-C----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-TC--C-C-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-G-G-A--C-A----G-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-T--TG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-TG------GA-G-G--------------G-G-T-A---GC-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC---AAT----G--GC-GAA-G--G-C----A-----A--G-C-T-A---CTG-----G--CC-A-T------------------------------------------------------------------------------G-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-T----A-TG--GG-G--AG-C-A-AA--CA

Otu0011631 M04041_72_000000000_AUMPE_1_2112_1939_15367 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-A-T--G-GAC----G--A-G--G---AA-TAAG-A---C-T-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CGG-T-TG-A-T-G-A-C-G-----G-T---A-G-TC--------C-A-T-GA----------A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AT-------G-CA-G-G-C-G---------------G--C-TT-T-G-T----------------------AA----G-T-C-T------------G-G-T--G--TG--T-AA-GA--C-C-AC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-G-G-G-G--A-G----C-G--C-A---C--T--------------G--GA-A-A---C---------------T--G-C--GA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-AG------GA-A-G--------------G-G-G-A---GT-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC---AGT----G--GC-GAA-G--G-C----G-----A--A-C-T-C---CTG-----G--CC-A-T------------------------------------------------------------------------------G-T------A-C-T--GA--CG---C----------------------------T-G--A-GA--T-G-CG-A--AA-G-C----G-TG--GA-G--AG-C-A-AA--CA

Otu0010074 M01867_242_000000000_ATWYL_1_1103_18150_8510 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-G-----A--T-GCC-G-C-G-T-----G-A-T--C--GA--------------------------T-G--A--A--G-G-TC---------GA-GC-------G-A-T--------T-G-T--A---AA-G-A-TC--------------------------TT-TT-A-C--A-GCT----G--A-G--G---AA-TAAG-T---C-T-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CGG-A-TG-A-T-G-A-C-G-----T-T---A-A-GC--------T-G-T-GA----------A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AA-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AC-------G-CA-G-G-C-G---------------G--C-CC-G-A-C----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-GA--C-C-TG-G-G-----------------------------------------------------CT-T-AA-----------C-T-C-A-G-G-G--A-C----C-G--C-G---T--T--------------G--GA-A-A---C---------------T--G-T--CG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--C-----T-TG------GA-G-G--------------G-G-G-A---GT-C--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-TG--AT-A-TT-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--A-C-T-C---CTG-----G--CC-G-A------------------------------------------------------------------------------A-G------A-T-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-C----A-TA--GG-G--AG-C-A-AA--CA

Otu0000425 M01867_242_000000000_ATWYL_1_1101_23803_4876 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--C--GA--------------------------T-G--A--A--G-G-CC---------GC-AA-------G-G-T--------T-G-T--A---AA-G-T-CC--------------------------TT-TT-G-C--A-GGT----G--A-A--G---AA-TAAG-C---C-G-G--G-G--A-------------------GT-G----------------------------GA--------------------------------A--------A---------------GC---C-CTG-G-TG-A-T-G-A-C-G-----T-T---A-G-CC--------T-G-C-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-CAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AG------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GT-------G-TA-G-G-C-G---------------G--C-CC-G-A-G----------------------AA----G-T-C-T------------G-G-T--G--TG--A-AA-TC--G-C-AG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-T-G-C-G--G-G----T-G--C-G---C--T--------------G--GA-A-A---C---------------T--G-A--CG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--C-----C-CG------GG-G-G--------------G-G-A-A---GC-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-GA-GT--G--------A-A-A---TC-T-GT-AG--AG-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----A--G-C-T-T---CTA-----T--TC-G-A------------------------------------------------------------------------------G-G------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000683 M01867_242_000000000_ATWYL_1_1102_3673_10450 .TAA--G-A-A--TC-TT--CC-A-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-T-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-A-T--A-GGC----G--A-G--G---AA-TAAG-A---T-C-C--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-GGG-T-CG-A-T-G-A-C-G-----G-T---A-G-CC--------T-G-T-GA----------A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-C-G---------------G--T-TC-G-A-C----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-GC--C-C-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-G-G-A--A-A----G-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-T--CG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-TG------GA-G-G--------------G-G-T-A---GC-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC---AAT----G--GC-GAA-G--G-C----A-----A--G-C-T-A---CTG-----G--CC-A-T------------------------------------------------------------------------------G-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-T----A-TG--GG-G--AG-C-A-AA--CA

Otu0000861 M01867_242_000000000_ATWYL_1_1107_13364_13191 .GAG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--A--T-G-CC---------GT-AA-------G-G-T--------T-G-T--A---AA-C-T-CC--------------------------TT-TT-G-G--G-AGA----G--A-T--G---AA-TAAC-T---G-C-G--ATT-------------------------A---------------------------GCA----------------------------------------------------------ATGGT-CGC-A-GC-C-T-G-A-C-C-----G-T---A-T-CT--------C-C-T-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-A-GGGG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--G-TT-G-G-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--T-C-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-G-A-A--A-C----T-G--C-C---T--C--------------T--GA-T-A---C---------------T--G-C--CA--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-AG------AA-G-A--------------G-A-G-A---GA-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-TG-GT--G--------A-A-A---TA-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----A--T-C-T-C---TTG-----G--TC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-T--AG-C-G-AA--CA

Otu0000908 M01867_242_000000000_ATWYL_1_1101_11941_9712 .TAA--G-A-A--TC-TT--GC-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--T--GA--------------------------T-G--A--A--G-G-CT---------TT-AG-------G-G-T--------T-G-T--A---AA-G-T-TC--------------------------AG-TA-A-G--C-AGG----G--A-A--G---AA-TAAG-C---A-A-G--A-G----------------------G--T----------------------------AA--------------------------------T---A----T----------------T---T-CTT-G-TG-A-T-G-A-C-G-----G-T---A-C-CT--------G-C-C-T---------A----------------AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GT-------C-TA-G-G-C-G---------------G--G-AA-A-T-T----------------------AA----G-T-T-A------------G-G-T--G--TG--A-AA-GC--T-A-TG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-A-T-A-A--A-C----T-G--C-A---T--C--------------T--AA-A-A---C---------------T--G-A--TT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-GG------GA-G-A--------------G-G-A-G---AG-C--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-T-C-T---CTG-----G--CC-T-A------------------------------------------------------------------------------G-A------A-C-T--GA--CG---C----------------------------T-G--A-AG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0001250 M01867_242_000000000_ATWYL_1_1109_23171_23028 .AGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-TT---------TT-CG-------G-A-T--------C-G-T--A---AA-C-T-TC--------------------------TT-TT-A-T--T-TGG----G--A-A--G---AA-ATCT-C---A-C-A--A----T-A-----------------G--G----------------------------AA--------------------------------A--------T---------------GA---T-TGT-G-AA-T-T-G-A-C-G-----G-T---A-C-CT--------T-A-T-G---------A-A-----------T--AA-------------GC-T--CC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GGA------------------GCG-A-G------------C--G--T---T--AC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GA-GT-TC-------G-TA-G-G-C-G---------------G--A-TT-C-G-T----------------------AA----G-T-C-A------------G-A-G--G--TG--A-AA-TT--T-T-GG-G-G-----------------------------------------------------CT-T-AA-----------C-C-C-C-G-A-A--A-C----T-G--C-C---T--T--------------T--GA-A-A---C---------------T--G-C--GT--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GT------GA-G-G--------------G-G-A-T---GA-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--T-C-A-T---CTG-----G--CA-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001312 M01867_242_000000000_ATWYL_1_1103_25788_11427 .GAG--G-G-A--TT-TT--CG-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-C-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-T-A--G-GGG----G--A-A--G---A----------------------------------------------G----------------------------AA--------------------------------C------------------------------------------G-G-A-C-G-----G-T---A-C-CC--------C-T-G-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCG-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-CA-G-G-C-G---------------G--T-TC-G-T-T----------------------AA----G-T-C-T------------G-G-G--G--TG--A-AA-TC--C-T-TC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-G-A-G-G--G-G----G-A--C-C---C--T--------------G--GA-T-A---C---------------T--G-G--CG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GG------GA-G-A--------------G-G-G-A---AG-C--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-A-CG-GT--G--------A-A-A---TG-T-AT-AG--AT-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-C---CTG-----G--AC-C-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----T-GG--GG-G--AG-C-G-AA--CA

Otu0001318 M01867_242_000000000_ATWYL_1_2112_23543_13402 .GGG--G-A-A--AC-CC--TG-A-C------GA-C-G-C--GA---------------------------CG-C--C--G-C--G-T-G-A-G-C-G-A---TG-A-----A--G-GCCTT-C-G-G-----GTT-G--T--AA--------------------------A-GC-T--C--T-G-TC---------AC-TA-------G-G-A--------A-G-G--A---AA-G-C-TT-------------------------TGAATGAA-T--A-ATT----C--A-G--T---A----------------------------------------------G----------------------------TT---------------------------------------------------------------------------T-G-A-C-A-----T-T---A-C-CT--------G-G-A-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-T-T---------------G--A-CA-A-G-A----------------------AA----G-T-G-A------------T-T-C--G--TG--A-AA-TC--C-T-AA-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-T-A-G-A--A-C----T-G--C-G---T--T--------------T--CA-A-A---C---------------T--T-C--TT--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-TG------AA-G-A--------------G-G-T-A---AG-T--------------AG--A--ATT-----------C-T-C-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------G-A---G-A--G-G-A-AT-A-CC---TTT----G--GT-GAA-G--A-C----G-----G--C-T-T-A---CTA-----T--TC-A-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AG-G-T----A-TG--GG-G--AG-C-A-AA--CA

Otu0001684 M01867_242_000000000_ATWYL_1_2107_6012_11761 .TAA--G-A-A--TC-TT--CC-A-C------AA-T-G-C--AC---------------------------GA-A--A--G-T--G-T-G-A-T-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-T--G--GA--------------------------T-G--A--A--G-C-CT---------CT-AG-------G-G-G--------T-G-T--A---AA-C-A-TC--------------------------TT-TT-A-T--T-TAG----G--A-A--A---A----------------------------------------------A----------------------------TT--------------------------------T---T--------------------------------------T-G-A-T-G-----T-T---A-C-TA--------A-A-T-G---------A-A-----------T--AA-------------GG-G--GC-T---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--AG---C-----------------GG--TA-AT-------AC---GA-AG-GCC------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GT-CT-------G-TA-G-A-C-G---------------G--T-TT-A-G-T----------------------AA----G-T-T-A------------A-T-C--G--TA--A-AA-TC--T-T-CT-A-G-----------------------------------------------------CT-C-AA-----------C-T-A-G-A-A-G--T-T----T-G--C-G---A--T--------------T--AA-T-A---C---------------T--A-T--TA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--T-----C-GG------GG-G-A--------------A-G-C-A---AG-T--------------GG--A--ATT-----------G-G-T-G-GT--GT-A-G-TC-GT--A--------A-T-A---AG-C-GT-TA--AT-A-TC-----------------------------A-C---C-A--G-G-A-AC-A-CC----GA-A--A--GC-GAA-A--G-C----A-----G--C-T-T-G---CTA-----A--AA-C-G------------------------------------------------------------------------------A-T------T-C-T--GA--CG---T----------------------------T-G--A-GA--G-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AT--AC

Otu0001950 M01867_242_000000000_ATWYL_1_2114_16244_20799 .AGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-TT---------TT-CG-------G-A-T--------C-G-T--A---AA-C-T-TC--------------------------TT-TT-A-T--T-TGG----G--A-A--G---AA--ATA-A---T-A-T--AG---T-A-----------------G--G----------------------------TA--------------------------------A--------T---------------GA---C-TAT-A-TG-T-T-G-A-C-G-----G-T---A-C-CT--------T-A-T-G---------A-A-----------T--AA-------------GC-T--CC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GGA------------------GCG-A-G------------C--G--T---T--AC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GA-GT-TC-------G-TA-G-G-C-G---------------G--A-TT-C-G-T----------------------AA----G-T-C-A------------G-A-G--G--TG--A-AA-TT--T-T-GG-G-G-----------------------------------------------------CT-T-AA-----------C-C-C-C-G-A-A--A-C----T-G--C-C---T--T--------------T--GA-A-A---C---------------T--G-C--GT--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GT------GA-G-A--------------G-G-A-A---GA-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--T-C-T-T---CTG-----G--CA-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002067 M01867_242_000000000_ATWYL_1_2114_12272_22809 .TAA--G-A-A--TC-TT--GG-A-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-T-C-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--T--GA--------------------------T-G--A--A--G-G-CT---------CT-CG-------G-G-T--------C-G-T--A---AA-A-T-TC--------------------------AC-TA-A-T--C-AGG----G--A-C--G---AA-TAAG-T---C-A-A--A-G----------------------C--T----------------------------AA--------------------------------T---A----T----------------C---T-TTG-A-TG-G-T-G-A-C-G-----G-T---A-C-CT--------G-G-C-T---------A----------------AA-------------GC-C--TC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GAG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------AT--A-----AA-GG-GC-AT-------C-TA-G-G-C-G---------------G--G-AA-A-T-T----------------------AA----G-T-C-G------------G-G-T--G--TG--A-AA-GC--C-C-GG-A-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-G-G-A--A-T----T-G--C-A---T--T--------------C--GA-T-A---C---------------T--G-A--TA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-GG------AA-G-A--------------G-G-A-A---GG-C--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-C-T-T---CTG-----G--TC-T-A------------------------------------------------------------------------------G-G------A-C-T--GA--CG---C----------------------------T-G--A-AG--T-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-G-AA--CG

Otu0002088 M01867_242_000000000_ATWYL_1_2108_25991_21637 .CGA--G-A-A--TC-TT--CC-A-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-T-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-T--T--GA--------------------------T-G--A--A--GCC-CC---------TT-TG-------G-G-G--------C-G-T--A---AA-G-A-TC--------------------------TT-TT-A-T--G-AGG----G--A-A--G---A----------------------------------------------AG---------------------------TT--------------------------------TA--T--------------------------------------T-G-ACG-GT----------A-C-CT--------C-A-T-G---------A-A-----------T--AA-------------GA-G--GC-T---C-C-TAA-----------------------T--C-T-C--G-T---------G-C-CA--G-C---A--G-G--AG---C-----------------GG--TA-AT-------AC---GA-GA-GCC------------------TCG-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-T-T---------------G--T-TC-T-G-T----------------------TA----G-T-C-T------------T-T-T--G--TC--A-AA-GC--C-C-CG-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-G-G-G-A--T-A----T-G--C-A---A--A--------------A--GA-A-A---C---------------G--A-C--AG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-A--G-----T-GC------GA-T-A--------------G-G-T-A---TA-C--------------AG--A--ACT-----------T-A-T-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-C-GT-TG--AT-A-TC-----------------------------A-T---A-G--G-G-A-AT-A-CC----AA-A--T--GC-GAA-G--G-C----A-----G--T-A-T-A---CTG-----G--CG-C-A------------------------------------------------------------------------------T-A------T-T-T--GA--CA---C----------------------------T-G--A-AG--C-A-CG-A--AA-G-C----G-TG--GG-T--AG-C-G-AA--TG

Otu0002199 M01867_242_000000000_ATWYL_1_1103_16510_16299 .CGA--G-A-A--TA-TT--TT-C-C------AA-T-G-C--GC---------------------------GA-A--A--G-C--G-T-G-A-G-A-A-T---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-C-T-TC--------------------------TT-TT-A-T--G-AGG----G--A-A--G---AA--AAGTC---A-G-G--G-G--T-------------------T--A----------------------------AT--------------------------------A---C----C---------------TT---C-TGA---CC-G-T-G-A-C-G-----T-T---A-C-CT--------T-A-T-G---------A-A-----------T--AA-------------GC-A--AC-G---G-C-TAA-----------------------A--C-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-GT-GTT------------------GCA-A-G------------C--G--T---T--AC-T-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GT-CA-------G-CA-G-G-C-G---------------G--C-TT-T-G-T----------------------AA----G-T-C-T------------T-A-G--G--TG--A-AA-TC--C-A-GC-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-C-T-G-A--A-C----G-G--C-C---T--A--------------A--GA-T-A---C---------------T--G-C--AT--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-GA------TA-G-A--------------G-G-A-G---AG-T--------------GG--A--ACA-----------G-C-T-A-GT--GT-A-A-AG-GT--G--------A-A-A---TT-T-GT-GG--AT-A-TT-----------------------------A-G---C-T--G-G-A-AC-G-CC----AA-C--G--GT-GAA-G--A-C----A-----G--C-T-C-T---CTG-----G--AT-C-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--G-A-CG-A--AA-G-C----G-TA--GG-G--AG-C-A-AA--CA

Otu0002412 M01867_242_000000000_ATWYL_1_1107_7092_13353 .GAG--G-G-A--TT-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-G-----A--C-ACC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TC-A-G--A-GGG----G--A-A--G---AG---------------------------------------------T----------------------------AA---------------------------------------------------------------------------G-G-A-C-G-----G-T---A-C-CC--------T-C-G-G---------A-G-----------G--AA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---GT-AG-GGG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GA-GC-TC-------G-TA-G-G-T-G---------------G--G-AC-T-A-T----------------------AA----G-T-C-G------------A-C-G--A--GT--A-AA-TA--T-C-TC-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-A-G-A-T--G-C----C-A--T-T---G--T--------------C--GA-T-A---C---------------T--G-T--GG--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--A-----C-AG------GA-G-A--------------G-G-A-A---AG-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-T---CTG-----G--CC-T-G------------------------------------------------------------------------------A-T------T-C-T--GA--CA---C----------------------------T-G--A-GG--A-G-CG-A--AA-G-C----C-AG--GG-G--AG-C-G-AA--CG

Otu0002469 M01867_242_000000000_ATWYL_1_1102_24599_4738 .CGA--G-A-A--TC-TT--TC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-A-C---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-CC--------------------------TG-TC-A-G--C-AGG----G--G-A--G---AA--CAA-G---C-T-G--TCG--A-------------------T--G----------------------------AA--------------------------------T---A----A---------------TC---G-ATA-G-CT-T-T-G-A-C-A-----G-T---A-C-CT--------G-C-A-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-CC-------G-TA-G-G-C-G---------------G--C-CT-G-A-A----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-TC--C-C-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-G-G-A--A-C----T-G--C-A---T--T--------------C--AA-A-A---C---------------T--T-T--CA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-T--G-----T-GA------TA-G-G--------------G-G-T-A---AA-G--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-A-GG-GT--G--------A-A-A---TC-T-GC-AG--AG-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--C--GC-GAA-G--G-C----G-----C--T-T-T-A---CTG-----G--AT-C-A------------------------------------------------------------------------------C-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--G-A-CG-A--AA-G-C----C-AG--GGG---AG-C-A-AA--CG

Otu0002707 M01867_242_000000000_ATWYL_1_1112_28259_17141 .AGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-TT---------TT-CG-------G-A-T--------C-G-T--A---AA-C-T-TC--------------------------TT-TT-A-T--T-TGG----G--A-A--G---AAT-AGC-T---A-T-T--G----TAG-----------------G--T----------------------------AA--------------------------------T--------G---------------AC---A--GT-A-GT-C-T-G-A-C-G-----G-T---A-C-CT--------T-A-T-G---------A-A-----------T--AA-------------GC-T--CC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GGA------------------GCG-A-G------------C--G--T---T--AC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GA-GT-TC-------G-TA-G-G-C-G---------------G--A-TT-C-G-T----------------------AA----G-T-C-A------------G-A-A--G--TG--A-AA-TT--T-T-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-C-G-A-A--A-C----T-G--C-T---T--T--------------T--GA-A-A---C---------------T--G-C--GT--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GT------GA-G-A--------------G-G-A-A---GA-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--T-C-T-T---CTG-----G--CA-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002843 M01867_242_000000000_ATWYL_1_1105_14752_15445 .CGA--G-A-A--TC-TT--TC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-A-C---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TC-A-C--T-AGG----A--A-G--G---AA--AGC-T---T---T--G-A--A-------------------T--G----------------------------AA--------------------------------T---A----A---------------TT---C-AGT-A-GT-T-T-G-A-C-A-----T-T---A-C-CT--------G-G-A-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-T-T---------------G--A-CA-G-G-C----------------------AA----G-T-G-A------------T-T-C--G--TG--A-AA-TC--C-T-AA-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-T-A-G-A--A-C----T-G--C-G---A--T--------------T--CA-A-A---C---------------T--G-C---T--T-G-T-------------------------------------------------------------------------------------------------------------------------------------------------------------------------CTT-G-A-G-T--A-----T-TG------AA-G-A--------------G-G-T-A---AG-T--------------AG--A--ATT-----------C-T-C-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------G-A---G-A--G-G-A-AT-A-CC----TT-T--G--GT-GAA-G--A-C----G-----G--C-T-T-A---CTA-----T--TC-A-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-GG--C-A-CG-A--AG-G-C----A-TG--GG-G--AG-C-A-AA--CA

Otu0003049 M01867_242_000000000_ATWYL_1_2107_16109_26629 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--A-G-CC---------GT-AA-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-C-T--A-GGA----G--A-T--G---AA-CGCC-C---T-G-C--A-TA------------------------G---------------------------GAA----------------------------------------------------------A--TGATGCAG-GA-A-T-G-A-C-C-----G-T---A-T-CC--------T-T-G-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-A-GGGG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GA-GC-------G-CA-G-G-C-G---------------G--G-CT-A-G-T----------------------AA----G-T-C-A------------G-A-G--G--TG--A-AA-TC--T-C-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-G-A-A--A-C----T-G--C-C---T--T--------------T--GA-T-A---C---------------T--G-C--TA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----T-GG------GA-G-G--------------G-A-G-A---GA-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-TG-GT--G--------A-A-A---TA-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----A--T-C-T-C---TTG-----G--CC-C-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-T-CG-A--AA-G-C----G-TG--GG-T--AG-C-G-AA--CA

Otu0003128 M01867_242_000000000_ATWYL_1_1109_9756_16846 .TAA--G-A-A--TC-TT--GG-A-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-T-C-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-A-T-TC--------------------------AC-TA-A-T--C-AGG----G--A-C--G---AA-TAAG-T---C-A-A--A-G----------------------C--T----------------------------AA--------------------------------T---A----T----------------C---T-TTG-A-TG-G-T-G-A-C-G-----G-T---A-C-CT--------G-G-C-T---------A----------------AA-------------GC-C--TC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GAG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------AT--A-----AA-GG-GC-AT-------C-TA-G-G-C-G---------------G--G-AT-A-T-T----------------------AA----G-T-C-G------------G-G-T--G--TG--A-AA-GC--C-T-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-C-A-G-A--A-C----T-G--C-A---T--T--------------C--GA-T-A---C---------------T--G-G--TA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-GG------AA-G-A--------------G-G-A-A---GG-C--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-C-T-T---CTG-----G--TC-T-T------------------------------------------------------------------------------G-G------A-C-T--GA--CG---C----------------------------T-G--A-AG--T-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-G-AA--CG

Otu0003503 M01867_242_000000000_ATWYL_1_1101_7554_8748 .TAA--G-A-A--TC-TT--GC-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--T--GA--------------------------T-G--A--A--G-G-CT---------TT-AG-------G-G-T--------T-G-T--A---AA-G-T-TC--------------------------AG-TA-A-G--C-AGG----G--A-A--G---AA-TAAG-C---G-T-G--G-G----------------------G--T----------------------------AA--------------------------------T---A----T----------------T---C-CAT-G-TG-A-T-G-A-C-G-----G-T---A-C-CT--------G-C-C-T---------A----------------AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GT-------C-TA-G-G-C-G---------------G--G-AA-A-T-T----------------------AA----G-T-T-A------------G-G-T--G--TG--A-AA-GC--T-A-TG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-A-T-A-A--A-C----T-G--C-A---T--C--------------T--AA-A-A---C---------------T--G-A--TT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-GG------GA-G-A--------------G-G-A-G---AG-C--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-T-C-T---CTG-----G--CC-T-A------------------------------------------------------------------------------G-A------A-C-T--GA--CG---C----------------------------T-G--A-AG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0003585 M01867_242_000000000_ATWYL_1_2107_6419_14493 .GGG--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-T-CC--------------------------TT-TC-G-A--A-TGG----G--A----A---TA--AGT-G---C-A-A--T-A--G-A-----------------C--G----------------------------AA--------------------------------T---A----G-------------T-TC---T-ATT-G-T--T-T-G-A-C-T-----G-T---A-C-CA--------T-A-G-G---------A-A-----------G--AA-------------GC-T--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTA------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-AC-GT-------G-TA-G-G-C-G---------------G--C-CA-T-A-T----------------------AA----G-T-C-A------------G-G-T--G--TG--A-AA-TC--G-T-TC-T-G-----------------------------------------------------CT-T-AA-----------C-A-G-A-A-C-A--A-T----T-G--C-A---C--C--------------C--GA-T-A---C---------------T--G-T--GT--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-AG------GA-G-G--------------G-G-A-A---AA-T--------------GG--A--ATT-----------T-C-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---A-A--A-G-A-AC-A-CC----CG-T--G--GC-GAA-G--G-C----G-----G--T-T-T-T---CTG-----G--CC-T-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-TA-A--AA-G-C----T-AG--GG-G--AG-C-A-AA--CG

Otu0003898 M01867_242_000000000_ATWYL_1_1108_7762_15344 .......................................................................TA-G--G--G-A--A-T-T-T-T-G-C-G---CA-A-----T--G-GGC-G-A-A-A-----G-C-C--T--GA--------------------------C-G--C--A--GCG-AC---------GC-CG-------C-G-T--------G-G-G--T---GA---C-GA--------------------------AA-TC-T-T--T-CGG----G--A-T--GT--AA-AACC-C---T-T-T--T-A--G-------------------C--A----------------------------GG--------------------------------A---A----C---------------G------AAA-A-AA-A-T-G-A-C-G-----G-T---A-C-CT--------G-C-A-G---------A-A-----------A--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---GG-GG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-AT-------G-CA-G-G-C-G---------------G--T-AA-A-T-T----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-TA--C-A-AA-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-T-T-G-T--T-G----T-G--C-A---T--C--------------A--GA-A-A---C---------------T--G-A--TT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----C-AA------TA-G-A--------------G-G-A-G---GG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-G-CC----AG-T--G--GC-GAA-A--G-C----G-----A--C-C-C-T---CTG-----G--GT-T-G------------------------------------------------------------------------------T-A------C-C-T--GA--CG---C----------------------------T-A--A-AA--T-A-CG-A--AA-G-C----T-CG--GG-G--AG-C-A-AA--CG

Otu0004053 M01867_242_000000000_ATWYL_1_1105_16371_23314 .CGA--G-G-A--TC-AT--TC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-T---GC-G-----A--C-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TG-TC-A-T--G-AGG----G--A-G--C---AA-AGAA-C---A-G-G--G-T--G-------------------T--G----------------------------AA--------------------------------T---A----G---------------CA---CCTTG---AA-G-T-G-A-T-A-----G-T---A-C-CT--------C-A-A-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GCG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-AT-C-G-C----------------------GT----G-T-C-G------------G-A-T--G--TG--A-AA-TC--T-C-AC-A-G-----------------------------------------------------CT-C-AA-----------C-T-G-T-G-G-A--A-T----G-G--C-A---T--T--------------C--GA-A-A---C---------------T--G-C--GG--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--A-----C-AG------GA-G-A--------------G-G-A-G---AG-C--------------GG--A--ATT-----------C-T-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------G-A---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-C-T---CTG-----G--AC-T-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----T-GG--GG-G--AG-C-A-AA--CA

Otu0004115 M01867_242_000000000_ATWYL_1_2111_26656_12987 .AGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-C-C-TC--------------------------TG-TT-G-G--G-TGG----G--A-A--G---AA-CAGG-A---T-G-T--G-T--G-------------------A--T----------------------------AA--------------------------------T---A----C---------------CG---C-ATG-T-CT-T-T-G-A-C-G-----G-T---A-C-CA--------C-C-T-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GA-GC-------G-TA-G-G-C-G---------------G--G-AG-T-G-T----------------------AA----G-T-T-A------------G-G-A--G--TG--A-AA-TT--T-A-TC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-A-T-A-A--C-T----T-G--C-T---T--T--------------T--AA-T-A---C---------------T--G-C--AT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GG------GA-G-A--------------G-G-A-T---GA-T--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AT-A-CC----AG-T--T--GC-GAA-G--G-C----G-----G--T-C-A-T---CTG-----G--CC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-T-CG-A--AA-G-C----T-AG--GG-G--AG-C-A-AA--CA

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Chapter 3

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Otu0004198 M01867_242_000000000_ATWYL_1_1101_22948_11068 .TAA--G-A-A--TC-TT--GG-A-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-T-C-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-A-T-TC--------------------------AC-TA-A-T--C-AGG----G--A-C--G---AA-TAAG-C-----A-C--G-G--G-------------------G--A----------------------------AA--------------------------------T---A----T---------------TC---C-GT--G-TG-A-T-G-A-C-A-----G-T---A-C-CT--------G-G-C-T---------A----------------AA-------------GC-C--AC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GTG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------AT--A-----AA-GG-GC-AT-------C-TA-G-G-C-G---------------G--G-AT-A-T-T----------------------AA----G-T-C-G------------G-G-T--G--TG--A-AA-GC--C-C-GG-A-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-G-G-A--A-C----T-G--C-A---T--T--------------C--GA-T-A---C---------------T--G-A--TA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-GG------AA-G-A--------------G-G-A-A---GG-C--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-C-T-T---CTG-----G--TC-T-A------------------------------------------------------------------------------G-A------A-C-T--GA--CG---C----------------------------T-G--A-AG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CG

Otu0004370 M01867_242_000000000_ATWYL_1_1112_7638_20779 .AGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-TT---------CT-CG-------G-A-T--------C-G-T--A---AA-C-T-TC--------------------------TT-TT-A-T--T-TGG----G--A-A--G---AA--ATG-C---TAT-T--G----T-A-----------------G--G----------------------------AA--------------------------------A--------T---------------GA---T-GAT-A-GT-T-T-G-A-C-G-----G-T---A-C-CT--------T-A-T-G---------A-A-----------T--AA-------------GC-T--CC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GGA------------------GCG-A-G------------C--G--T---T--AC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GA-GT-TC-------G-TA-G-G-C-G---------------G--A-TT-A-G-T----------------------AA----G-T-C-A------------G-A-A--G--TG--A-AA-TT--T-T-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-C-G-A-A--A-C----T-G--C-T---T--T--------------T--GA-A-A---C---------------T--G-C--TT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GT------GA-G-A--------------G-G-A-T---GA-T--------------GG--A--ATT-----------T-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--T-C-A-T---CTG-----G--CA-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004445 M01867_242_000000000_ATWYL_1_2112_20733_8470 .AAG--G-G-A--TA-TT-GCG-A-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--C--G-G-CT---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-TC--------------------------TT-TT-C-G--G-TGG----G--A-A--G---A----------------------------------------------G----------------------------AC--------------------------------A------------------------------------------A-G-A-C-G-----G-T---A-C-CA--------C-C-G-G---------A-A-----------G--AA-------------GT-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GC-GT-GT-------G-CA-G-G-C-G---------------G--T-CA-T-G-T----------------------AA----G-T-A-G------------C-G-C--G--TG--A-AA-GC--G-C-CC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-G-G-C-G--A-G----G-A--C-G---T--G--------------C--TG-T-A---C---------------T--G-C--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-G--C-----T-GG------TA-G-A--------------G-G-C-G---AG-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC----TG-A--G--GC-GAA-G--G-C----G-----A--C-T-C-G---CTG-----G--GC-C-A------------------------------------------------------------------------------G-T------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-A-CG-A--CA-G-C----A-TG--GG-G--AG-C-G-AA--CG

Otu0004762 M01867_242_000000000_ATWYL_1_1107_20234_18174 .TTC--G-A-A--TT-TT--TC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--G--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-C-C-CC--------------------------TG-TC-A-G--C-TGG----G--A-A--C---AA-GGTC-T-----C-G--T-G--G-------------------T--G----------------------------AA--------------------------------T---A----G---------------CC---A-TGA-G-AT-T-T-G-A-T-A-----G-T---A-C-CG--------G-C-A-G---------A-G-----------G--AA-------------GC-C--GT-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------TC---AG-AG-ACG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-CC-------G-CA-G-G-T-G---------------G--C-GA-A-G-C----------------------AA----G-T-C-T------------G-A-C--G--TG--A-AA-TC--C-C-GC-G-G-----------------------------------------------------CC-T-AA-----------C-C-G-C-G-G-A--A-C----T-G--C-G---T--C--------------G--GA-T-A---C---------------T--G-C--CT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----C-GG------CA-G-A--------------G-G-A-G---AC-T--------------GG--A--ATT-----------C-A-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-T---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----A--G-T-C-T---CTG-----G--AC-C-G------------------------------------------------------------------------------T-T------C-C-T--GA--CA---C----------------------------T-C--A-GG--G-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CG

Otu0004791 M01867_242_000000000_ATWYL_1_2112_22257_6619 .AGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--A--G-G-CT---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-TC--------------------------TG-TT-G-G--C-AGG----G--A-A--G---AA-A-AG-C---T-T-G--G-T--G---------------------------------------------------AA--------------------------------G---A---------------------G---C-CAA-G-T--C-T-G-A-C-G-----G-T---A-C-CT--------G-C-T-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GA-GC-------G-TA-G-G-C-G---------------G--G-GA-A-G-T----------------------AA----G-T-T-A------------G-G-A--G--TG--A-AA-TT--T-A-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-T-A-A--C-G----T-G--C-T---T--T--------------T--AA-T-A---C---------------T--G-C--TT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-TG------GA-G-A--------------G-G-T-A---GA-C--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----AG-T--T--GC-GAA-G--G-C----G-----G--T-C-T-A---CTG-----G--CC-A-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-T-CG-A--AA-G-G----T-AG--GG-G--AG-C-A-AA--CA

Otu0005239 M01867_242_000000000_ATWYL_1_2106_17826_25580 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--GA---------------------------GA-A--A--T-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--T--GA--------------------------T-G--A--A--G-G-CA---------GA-AA-------T-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-A-T--A-CGG----G--A-A--G---AA-TAAG-G---A-T-A--G-G--A-------------------GT-G----------------------------GA--------------------------------A--------A---------------GC---C-TAT-T-CG-A-T-G-A-C-G-----G-T---A-C-CG--------T-A-T-G---------A-A-----------T--AA-------------GC-T--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGA------------------GCT-A-G------------C--G--T---T--GT-T-CGG-AT------TC-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-AC-------G-TA-G-G-C-G---------------G--T-TA-T-A-A----------------------AA----G-T-G-T------------G-G-A--T--TT--A-AA-GA--C-C-GA-G-G-----------------------------------------------------CT-T-AA-----------C-C-T-C-G-T-G--T-A----T-G--G-T---C--T--------------G--CA-A-A---C---------------T--A-T--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-AG------GA-G-G--------------G-G-A-A---GC-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AG-A-TG-----------------------------T-G---G-A--A-G-A-AT-A-CC----GG-T--G--GC-GAA-G--G-C----G-----A--G-C-T-T---CTG-----G--CC-T-G------------------------------------------------------------------------------A-A------A-C-T--GA--CG---C----------------------------T-A--A-TG--T-A-CG-A--AA-G-T----G-TG--GG-G--AT-C-A-AA--CA

Otu0005777 M01867_242_000000000_ATWYL_1_1104_17457_23105 .AAA--G-C-C--TG-AC--GG-A-G------CG-A-C-G--CC---------------------------GC-G--T--G-A--A-T-G-A-T-G-A-A---GT-C-----T--T-TCG-G-G-A-T-----G-T-A--A--AG--------------------------T-T--C--A--A-T-CG---------AC-CG-------G-G-G--------A-A-G--A---AT-G-G-TT--------------------------TG-CA-T-A--CTAAT----A--ATAT-G---C-------------------------------------------G--A----------------------------GC--------------------------------A---G--------------------------------------A-G-A-C-G-----G-T---A-C-CC--------G---G-A---------A-T-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AA-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--T-AA-T-A-T----------------------AA----G-T-T-T------------T-G-C--G--TT--T-AA-GG--C-G-GA-G-G-----------------------------------------------------CT-C-AA-----------C-T-T-C-C-G-T--T-A----T-G--C-G---T--G--------------A--AA-G-A---C---------------T--G-T--AT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----A-CG------AA-G-A--------------G-G-A-A---AC-T--------------GG--A--ACT-----------C-C-A-G-GT--GA-A-G-CG-GT--G--------A-A-A---TG-T-GT-AG--AT-A-TC-----------------------------T-G---G-G--G-G-A-AC-A-CC----AA-T--G--GC-GAA-G--G-C----A-----G--G-T-T-T---CTG-----G--TT-G-T------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CG

Otu0006034 M01867_242_000000000_ATWYL_1_1102_6413_18026 .AAG--G-G-A--TA-TT-GCG-A-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--C--G-G-CT---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-TC--------------------------TT-TT-C-T--G-TGG----G--A-A--G---A----------------------------------------------G----------------------------AC--------------------------------A------------------------------------------G-G-A-C-G-----G-T---A-C-CA--------C-A-G-G---------A-A-----------G--AA-------------GT-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GC-GT-GT-------G-CA-G-G-C-G---------------G--T-CA-T-G-T----------------------AA----G-T-A-G------------C-G-C--G--TG--A-AA-GC--G-C-CC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-G-G-C-G--A-G----G-A--C-G---T--G--------------C--TG-T-A---C---------------T--G-C--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-G--C-----T-GG------TA-G-A--------------G-G-C-G---AG-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC----TG-A--G--GC-GAA-G--G-C----G-----A--C-T-C-G---CTG-----G--GC-C-A------------------------------------------------------------------------------G-T------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-A-CG-A--CA-G-C----A-TG--GG-G--AG-C-G-AA--CG

Otu0006705 M01867_242_000000000_ATWYL_1_1105_23043_19878 .CGA--G-A-A--TC-TT--CC-A-C------AA-T-G-C--GC---------------------------GA-A--A--G-C--G-T-G-A-T-G-G-A---GC-G-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TG-TC-A-C--A-GGG----G--A-C--G---AA--------------------------------------------------------------------------TA--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-TC--------T-G-G-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-CC-------G-CA-G-G-G-G---------------G--C-TA-A-T-T----------------------AA----G-T-C-T------------G-G-T--G--TG--A-AA-TC--T-C-TC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-A-G-A-A--A-C----T-G--C-A---T--T--------------G--GA-T-A---C---------------T--G-A--TT--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-C--A-----T-CG------GA-G-A--------------G-G-A-G---AA-T--------------GG--A--ATT-----------C-C-T-G-GA--GT-A-G-CG-GT--A--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-TC---AAA----A--GC-GAA-G--G-C----A-----G--T-T-C-T---CTG-----G--AC-G-A------------------------------------------------------------------------------T-A------G-C-T--GA--CC---C----------------------------T-C--A-GG--G-A-CG-A--AA-G-C----G-TG--GG-T--AG-C-A-AA--CA

Otu0007045 M01867_242_000000000_ATWYL_1_1111_18893_22128 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--GA---------------------------GA-T--A--T-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------A-G--A--A--G-G-CA---------GA-AA-------T-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-G-T--A-CGG----G--A-C--G---AA-TAAA-T---A-T-A--G-T--A-------------------GT-G----------------------------GA--------------------------------A--------A---------------GA---C-TAT-A-TG-A-T-G-A-C-G-----G-T---A-C-TG--------T-A-T-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-TT-------G-TA-G-G-T-G---------------G--T-TA-T-T-T----------------------AA----G-T-G-C------------G-A-G--T--TG--A-AA-GA--C-C-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-T-G-A-G--T-T----T-G--G-C---T--T--------------G--CA-A-A---C---------------T--G-A--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-AG------AA-G-G--------------G-G-T-A---AC-T--------------GG--A--ATT-----------C-C-G-C-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TG-----------------------------C-G---G-A--A-G-A-AT-A-CT----TG-T--G--GC-GAA-G--G-C----G-----A--G-T-T-A---CTG-----G--TC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CA---C----------------------------T-G--A-AA--A-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0007121 M01867_242_000000000_ATWYL_1_2113_7071_14253 .AGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--T--GA--------------------------C-G--A--A--G-C-CC---------TT-CG-------G-G-G--------T-G-T--A---AA-G-T-TC--------------------------TG-TC---A--G-TGG----G-------G----A-C--------------------G-----------------------------------------------------------------------------------------A---------------------A-------T-T-T----T-G-A-C-G-----G-T---A-C-CC--------A-C-A-A---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TC-A--T-T--GG-GC-------------------GT--A-----AA-GC-GT-TC-------G-TA-G-G-C-G---------------G--C-AT-A-T-C----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-GC--C-C-GG-G-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-G-G-T--T-C----G-G--C-A---C--T--------------G--GA-T-A---C---------------T--G-G--TA--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--G-----A-GG------TA-G-A--------------G-G-T-A---AT-G--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GTA-A--G-C----G-----C--A-T-T-A---CTG-----G--GC-C-T------------------------------------------------------------------------------T-T------A-T-T--GA--CG---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----C-GG--GG-G--AG-C-A-AA--TG

Otu0007125 M01867_242_000000000_ATWYL_1_1101_13412_9599 .AGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-C-C-TC--------------------------TG-TTGA-C--A-GGGAAGAAAAGCATGGTGAAG------------------------------------------A--GC---------------------------CA--------------------------------T---GTA------------------------------------T-G-A-C-G-----G-T---A-C-CT--------G-T-T-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GA-GT-------G-TA-G-G-C-G---------------G--G-AG-A-G-T----------------------AA----G-T-T-A------------G-G-A--G--TG--A-AA-TG--T-A-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-T-A-A--C-G----T-G--C-T---T--T--------------T--AA-T-A---C---------------T--G-C--TT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-TG------GA-G-A--------------G-G-T-A---AA-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC----AG-T--T--GC-GAA-G--G-C----G-----G--T-T-T-A---CTG-----G--CC-A-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-T-CG-A--AA-G-G----T-AG--GG-G--AG-C-A-AA--CA

Otu0007508 M01867_242_000000000_ATWYL_1_2109_26187_14772 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--GA---------------------------GC-A--A--T-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------T-G--A--A--G-G-CA---------GA-AA-------T-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-T-A--A-CGG----G--A-A--G---AA-TAAG-T---A-TAG--T-G--A-------------------G--A----------------------------GA--------------------------------A--------A---------------CG---C-TAT-A-TG-A-T-G-A-C-G-----G-T---A-C-CG--------T-T-A-G---------A-A-----------T--AA-------------GT-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------ACA-A-G------------C--G--T---T--GT-T-CGG-AT------TC-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AT-------G-TA-G-G-C-G---------------G--T-TT-A-A-T----------------------AA----G-T-G-T------------G-A-A--T--TG--A-AA-GA--C-C-GG-G-G-----------------------------------------------------CT-T-AA-----------C-C-C-C-G-A-G--T-T----T-G--G-T---T--T--------------G--CA-A-A---C---------------T--G-T--TA--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-GG------GA-G-A--------------G-G-A-G---AC-T--------------GG--A--ATT-----------C-C-G-T-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TG-----------------------------C-G---G-A--A-G-A-AT-A-CT----TA-T--G--GC-GAA-G--G-C----A-----A--G-T-C-T---CTG-----G--CC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-AA--T-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0007527 M01867_242_000000000_ATWYL_1_1110_25267_11259 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-T-G--G--GA--------------------------T-G--A--A--G-G-TC---------TT-CGT------G-A-T--------C-G-T--A---AA-C-C-AC--------------------------TG-TC-A-G--A-GGG----G--A-T--T---AA-ATGT-A---T-G-T--G-G--A-------------------A--T----------------------------AA--------------------------------T---A----C---------------TT---C-ATG-T-AC-T-T-G-A-C-T-----T-A---A-C-CC--------T-C-A-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGG------------------GCT-A-G------------C--G--T---T--AC-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GA-GA-AT-------T-CA-G-G-C-T---------------G--T-CT-T-G-T----------------------AA----G-T-C-A------------G-A-G--G--TG--A-AA-TC--C-C-TC-A-G-----------------------------------------------------CT-C-AA-----------C-T-G-A-G-G-A--A-C----T-G--C-C---T--T--------------T--GA-T-A---C---------------T--G-C--TG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--A-----C-AG------GA-G-A--------------G-G-A-A---AG-T--------------AG--A--ATT-----------C-C-C-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AT-A-CC----TG-A--G--GC-GAA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-T-G------------------------------------------------------------------------------G-T------C-C-T--GA--CG---C----------------------------T-G--A-TA--T-T-CG-A--AA-G-C----T-AG--GG-G--AG-C-A-AA--CA

Otu0007649 M01867_242_000000000_ATWYL_1_1108_17986_10023 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-G-----A--T-GCC-G-C-G-T-----G-A-T--C--GA--------------------------T-G--A--A--G-G-TC---------GA-GA-------G-A-T--------T-G-T--A---AA-G-A-TC--------------------------TT-TT-C-C--A-ACT----G--A-G--G---AA-TAAG-T---G-T-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CGC-A-TG-A-T-G-A-C-G-----T-T---A-A-GT--------T-G-G-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AA-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AC-------G-CA-G-G-C-G---------------G--T-TC-T-G-C----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-TC--T-C-CG-G-G-----------------------------------------------------CT-T-AA-----------C-T-C-G-G-A-A--T-C----C-G--C-G---T--T--------------G--GA-A-A---C---------------T--G-C--AA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-TA------GA-G-G--------------G-G-G-A---GT-C--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-TG--AT-A-TT-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--A-C-T-C---CTG-----G--CT-G-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-C----A-TA--GG-G--AG-C-A-AA--CA

Otu0008269 M01867_242_000000000_ATWYL_1_1105_8926_17713 .AGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-T-G--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-AC--------------------------TG-TT-G-G--G-TGG----G--A-A--G---AA-TCTA-C---G-G-T--G-T--A-------------------G--G----------------------------A---------------------------------A---A----T---------------GA---C-ATC-G-TA-C-T-G-A-C-G-----G-T---A-C-CA--------C-C-T-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GA-GT-------G-TA-G-G-C-G---------------G--G-AG-A-G-T----------------------AA----G-T-T-A------------G-G-A--G--TG--A-AA-TT--T-A-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-T-A-A--C-G----T-G--C-T---T--T--------------T--AA-T-A---C---------------T--G-C--TT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GG------GA-G-A--------------G-G-T-G---AG-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC----AG-T--T--GC-GAA-G--G-C----G-----G--C-T-C-A---CTG-----G--CC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-T-CG-A--AA-G-C----T-AG--GG-G--AG-C-A-AA--CA

Otu0008587 M01867_242_000000000_ATWYL_1_2108_17413_21455 .TAA--G-A-A--TC-TT--GG-A-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-T-C-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-G-T-TC--------------------------AG-TA-A-G--C-AGG----G-----------AC-GAA--C---A---------------------------------------------------------------C---------------------------------A----------------------------------------A-T-G-A-C-G-----G-T---A-C-CT----------G-C-C---------T-A--------------AA-------------GC-C--TC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GAG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AT-------T-CA-G-G-C-G---------------G--G-GT-G-T-T----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TC--T-C-AA-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-T-G-A-A--A-C----T-G--C-A---T--C--------------C--GA-T-A---C---------------T--G-G--CG--C-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--C-----A-GG------GA-G-A--------------G-G-A-A---GG-C--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-T-T---CTG-----G--CC-T-A------------------------------------------------------------------------------T-G------A-C-T--GA--CG---C----------------------------T-G--A-AG--T-G-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CG

Otu0008653 M01867_242_000000000_ATWYL_1_1109_18266_18624 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-G-----A--T-GCC-G-C-G-T-----G-A-T--C--GA--------------------------T-G--A--A--G-G-TC---------GA-GA-------G-A-T--------T-G-T--A---AA-G-A-TC--------------------------TT-TT-C-C--A-ACT----G--A-G--G---AA-TAAG-C---A-T-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CGT-G-TG-A-T-G-A-C-G-----T-T---A-A-GT--------T-G-G-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AA-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AC-------G-CA-G-G-C-G---------------G--T-TA-T-G-C----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-TC--T-C-CG-G-G-----------------------------------------------------CT-T-AA-----------C-T-C-G-G-A-A--T-C----C-G--C-G---T--T--------------G--GA-A-A---C---------------T--G-C--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-TA------GA-G-G--------------G-G-G-A---GT-C--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-TG--AT-A-TT-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--A-C-T-C---CTG-----G--CT-G-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-C----A-TA--GG-G--AG-C-A-AA--CA

Otu0009034 M01867_242_000000000_ATWYL_1_1114_14033_8528 .AGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--C--G-G--C---C-----TT-AG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TGTTGAG-T--GGGAAG---A--A-C--C-AGCAG-TGTGC---T-A-A------------------------------T---------------------------CAG--------------------------------C-------------------------------ATT-G-CT-C-T-G-A-C-G-----G-T---A-C-CA--------C-T-T-T---------A-G-----------A--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GC-GA-AC-------G-TA-G-G-C-G---------------G--G-GA-A-G-T----------------------AA----G-T-T-A------------G-G-A--G--TG--A-AA-TT--T-A-TC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-G-T-A-A--T-C----T-G--C-T---T--T--------------T--AA-T-A---C---------------T--G-C--TT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GG------GA-G-A--------------G-G-A-T---GA-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----AG-T--T--GC-GAA-G--G-C----G-----G--T-C-A-T---CTG-----G--CC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--T-T-CG-A--AA-G-C----T-AG--GG-G--AG-C-G-AA--CA

Otu0009230 M01867_242_000000000_ATWYL_1_2107_7725_15601 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--GA---------------------------GA-T--A--T-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------T-G--A--A--G-G-CA---------GA-AA-------T-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-A-T--A-GGT----G--A-C--G---AA-TAAG-A---GTA-C--T-G--A-------------------G--A----------------------------GA--------------------------------A--------A---------------CG---G-TAC-T-TG-A-T-G-A-C-G-----G-T---A-A-CT--------T-A-T-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AT-------G-TA-G-G-C-G---------------G--T-TA-A-A-A----------------------AA----G-T-G-T------------G-A-A--T--TG--A-AA-GA--C-C-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-G-T-G--T-T----T-G--G-T---T--T--------------G--CA-A-A---C---------------T--G-T--TT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--A-----C-AG------GA-G-G--------------G-G-T-G---AC-T--------------GG--A--ATT-----------C-C-G-C-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TG-----------------------------C-G---G-A--A-G-A-AT-A-CT----TG-T--G--GC-GAA-G--G-C----G-----A--G-T-C-A---CTG-----G--CC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-AA--T-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0009578 M01867_242_000000000_ATWYL_1_2112_11172_13889 .AGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-TT---------TT-CG-------G-A-T--------C-G-T--A---AA-C-T-TC--------------------------TT-TT-A-T--T-TGG----G--A-A--G---AAT-AGC-T---A-T-T--G----TAG-----------------G--T----------------------------AA--------------------------------T--------G---------------AC---A--GT-A-GT-C-T-G-A-C-G-----G-T---A-C-CA--------T-T-T-G---------A-A-----------T--AA-------------GC-T--CC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GGA------------------GCG-A-G------------C--G--T---T--AC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GA-GT-TC-------G-TA-G-G-C-G---------------G--A-TT-C-G-T----------------------AA----G-T-C-A------------G-A-A--G--TG--A-AA-TT--T-T-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-C-G-A-A--A-C----T-G--C-T---T--T--------------T--GA-A-A---C---------------T--G-C--GT--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GT------GA-G-A--------------G-G-A-T---GA-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--T-C-A-T---CTG-----G--CA-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0009597 M01867_242_000000000_ATWYL_1_2101_12424_9820 .AGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-TT---------TT-CG-------G-A-T--------C-G-T--A---AA-C-T-TC--------------------------TT-TT-A-T--T-TGG----G--A-A--G---AA-ATAT-C---A-C-A--G----C-A-----------------G--G----------------------------AA--------------------------------A--------T---------------GG---T-TGT-G-AG-T-T-G-A-C-G-----G-T---A-C-CT--------T-A-T-G---------A-A-----------T--AA-------------GC-T--CC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GGA------------------GCG-A-G------------C--G--T---T--AC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GA-GT-TC-------G-TA-G-G-C-G---------------G--A-TT-A-G-T----------------------AA----G-T-C-A------------G-A-A--G--TG--A-AA-TT--T-T-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-C-G-A-A--A-C----T-G--C-T---T--T--------------T--GA-A-A---C---------------T--G-C--TT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GT------GA-G-A--------------G-G-A-T---GA-T--------------GG--A--ATT-----------T-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--T-C-A-T---CTG-----G--CA-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0009937 M01867_242_000000000_ATWYL_1_1114_24284_17115 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--GA---------------------------GA-T--A--T-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-A-T--A-CGG----G--A-A--G---AATAAAG-A---C-A-C--T-G--A-------------------G--A----------------------------GA--------------------------------A--------A---------------CG---G-TGT-A-AG-A-T-G-A-C-T-----G-T---A-C-CG--------T-A-T-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-TT-------G-TA-G-G-T-G---------------G--C-GA-T-A-T----------------------AA----G-T-G-T------------G-A-G--T--TG--A-AA-GA--C-C-GA-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-C-G-A-G--T-T----T-G--G-C---T--T--------------G--CA-A-A---C---------------T--G-T--AT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-AG------GA-G-G--------------G-G-T-G---AC-T--------------GG--A--ATT-----------C-C-A-T-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TA-----------------------------T-G---G-A--A-G-A-AT-A-CT----TG-T--G--GC-GAA-G--G-C----G-----A--G-T-C-A---CTG-----G--CC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-AA--A-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0010053 M01867_242_000000000_ATWYL_1_1104_22329_5456 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--A--G-G-TC---------TT-CGT------G-A-T--------C-G-T--A---AA-C-C-TC--------------------------TG-TC-A-G--G-AAG----G--A-T--T----A-ATGT-A---T-T-T--T-G--G-------------------A--T----------------------------AA--------------------------------T---A----C---------------CC---A-AGG-T-GC-T-T-G-A-C-T-----T-A---A-C-TT--------C-C-A-A---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGG------------------GCA-A-G------------C--G--C---T--AC-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GA-GC-------G-TA-G-G-C-G---------------G--T-CT-G-A-T----------------------AA----G-T-C-A------------G-G-G--G--TG--A-AA-TC--C-C-TC-A-G-----------------------------------------------------CT-C-AA-----------C-T-G-A-G-G-A--A-C----T-G--C-C---C--T--------------T--GA-T-A---C---------------T--G-T--TA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----C-AG------GA-G-A--------------G-G-A-A---AG-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-A--G--GC-GAA-G--G-C----G-----A--C-T-T-T---CTG-----G--AC-T-G------------------------------------------------------------------------------A-T------C-C-T--GA--CG---C----------------------------T-G--A-GG--T-T-CG-A--AA-G-C----T-AG--GG-G--AG-C-A-AA--CA

Otu0010349 M01867_242_000000000_ATWYL_1_1107_11781_18752 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--GA---------------------------GA-T--A--T-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------A-G--A--A--G-G-CA---------GA-AA-------T-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-A-T--A-CGG----G--A-C--G---AA-TAAG-T---A-T-AT-T-G--A-------------------G--A----------------------------GA--------------------------------A--------A---------------CC---T-TAT-A-CG-A-T-G-A-C-G-----G-T---A-C-CG--------T-A-T-G---------A-A-----------T--AA-------------GT-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------ACA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AT-------G-TA-G-G-C-G---------------G--C-GA-G-A-T----------------------AA----G-T-G-T------------G-A-A--T--TG--A-AA-GA--C-C-GA-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-C-G-C-G--T-T----T-G--G-T---T--T--------------G--CA-A-A---C---------------T--G-T--CT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--A-----T-GG------AA-G-G--------------G-G-A-G---AC-T--------------GG--A--ATT-----------C-C-G-C-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TG-----------------------------C-G---G-A--A-G-A-AT-A-CT---TGT----G--GC-GAA-G--G-C----G-----A--G-T-C-T---CTA-----G--TC-T-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-AA--T-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0011275 M01867_242_000000000_ATWYL_1_1102_7373_15929 .CAA--G-A-A--TC-TT--CC-A-C------AA-T-G-G--CC---------------------------GA-A--A--G-G--C-T-G-A-T-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-C-A--G--GA--------------------------A-G--A--C--G-C-CC---------TT-CG-------G-G-G--------T-G-T--A---AA-C-T-GC--------------------------TT-TT-C-T--C-AGG----G--A-C--G---AA---------------------------------------------T----------------------------TC-------------------------------------------------------------------------G-T-G-A-C-G-----G-T---A-C-CT--------G-A-G-G---------A-A-----------T--AA-------------GA-G--GT-T---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--AG---C-----------------GG--TA-AT-------AC---AG-AG-ACC------------------TCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-CC-------G-TA-G-G-T-G---------------G--G-TT-G-G-T----------------------AA----G-T-T-A------------G-T-G--G--TT--A-AA-TC--C-T-GG-G-G-----------------------------------------------------CT-T-AA-----------C-T-T-C-A-G-G--A-C----T-G--C-C---G--C--------------T--AA-T-A---C---------------T--G-T--CA--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----T-GG------TA-G-A--------------A-G-C-A---AG-C--------------GG--A--ACT-----------A-C-C-G-GT--GT-A-G-TC-GT--A--------A-T-A---AG-C-GC-TG--AT-A-TC-----------------------------G-G---T-A--G-G-A-AC-A-CC----AA-A--G--GC-AAA-G--G-C----A-----G--C-T-T-G---CTA-----T--AA-C-A------------------------------------------------------------------------------A-A------C-C-T--GA--CA---C----------------------------T-C--A-AG--G-A-CT-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--TA

Otu0011424 M01867_242_000000000_ATWYL_1_2106_21086_18514 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--GA---------------------------GA-A--A--T-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--C--GA--------------------------C-G--A--A--G-G-CA---------GA-AA-------T-G-T--------T-G-T--A---AA-G-T-CC--------------------------TT-TT-A-T--A-TGG----G--A-A--G---AA-TAAG-G---A-T-A--G-T--A-------------------GT-G----------------------------GA--------------------------------A--------T---------------GA---T-TAT-T-CG-A-T-G-A-C-G-----G-T---A-C-CA--------T-A-T-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--T-TA-T-A-T----------------------AA----G-T-G-T------------G-G-A--T--TT--A-AA-TA--T-C-TG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-A-G-A-A--T-C----T-G--G-T---C--T--------------G--CA-A-A---C---------------T--G-T--AT--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----T-AG------GA-T-G--------------G-G-A-A---AC-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AG-A-TG-----------------------------T-G---G-A--A-G-A-AT-A-CC----GG-T--G--GC-GAA-G--G-C----G-----A--G-T-T-T---CTG-----G--CC-T-A------------------------------------------------------------------------------A-A------A-C-T--GA--CG---C----------------------------T-G--A-AA--C-A-CG-A--AA-G-T----G-CG--GG-G--AG-C-A-AA--CA

Otu0011516 M01867_242_000000000_ATWYL_1_1102_20685_17724 .AGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-T-G--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-AC--------------------------TG-TT-G-G--G-TGG----G--A-A--G---AA-CCTG-T---G-T-C--G-C--A-------------------G--G----------------------------A---------------------------------A---A----T---------------GG---C-GAT-G-CA-C-T-G-A-C-G-----G-T---A-C-CA--------C-C-T-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GA-GT-------G-TA-G-G-C-G---------------G--G-AG-A-G-T----------------------AA----G-T-T-A------------G-G-A--G--TG--A-AA-TT--T-A-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-T-A-A--C-G----T-G--C-T---T--T--------------T--AA-T-A---C---------------T--G-C--TT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GG------GA-G-A--------------G-G-T-G---AG-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC----AG-T--T--GC-GAA-G--G-C----G-----G--C-T-C-A---CTG-----G--CC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-T-CG-A--AA-G-C----T-AG--GG-G--AG-C-A-AA--CA

Otu0011780 M01867_242_000000000_ATWYL_1_1101_11202_5635 .AGG--G-A-A--TT-TT--CC-A-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-G-G-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--A--G-C-AT---------TT-CG-------G-T-G--------T-G-T--A---AA-C-C-TC--------------------------TT-TG-G-A--G-CGG----G--A-A--G---AA-TGCCGTTT-G-G-G--T---------------------------G----------------------------AA--------------------------------C---AG------------------------T-CCAGACGG-G-T-G-A-C-G-----G-T---A-C-CG--------C-T-A-G---------A-A-----------C--AA-------------GA-C--AC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GTG------------------TCA-A-A------------C--G--T---T--GT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-CA-G-G-T----------------------AA----G-T-G-A------------G-A-T--G--TG--A-AA-GC--T-C-TG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-A-G-G-A--A------T-TG-C-A---T--T--------------T--CA-T-A---C---------------T--G-C--TT--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-GG------TA-G-A--------------G-G-A-G---GG-C--------------GG--A--ATT-----------C-C-A-G-AA--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--G-G-A-AC-A-CC---CGT----G--GC-GAA-G--G-C----G-----G--C-T-C-T---CTG-----G--TC-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-G----T-AG--GG-G--AG-C-A-AA--CA

Otu0013665 M01867_242_000000000_ATWYL_1_1106_26508_13816 .CAA--G-A-A--TA-TT--CC-T-C------AA-T-G-G--CC---------------------------GA-A--A--G-G--C-T-G-A-A-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-T-A--C--GA--------------------------A-G--A--C--G-C-CC---------TC-CG-------G-G-G--------T-G-T--A---AA-G-T-AC--------------------------TT-TT-A-A--C-TGG----G--A-A--A---A----------------------------------------------AT---------------------------TT------------------------------------A--------------------------------------T-G-A-T-G-----G-T---A-C-CA--------G-T-T-G---------A-A-----------TA-A--------------GG-G--GC-T---G-C-TAA-----------------------C--C-T-C--G-T---------G-C-CA--G-C---A--G-C--AG---C-----------------GG--TA-AT-------AC---GA-GG-GCC------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-CC-------G-TA-G-G-T-G---------------G--T-TT-T-T-TC---------------------GC----A-T-C-T------------C-C-T--G--TT--A-AA-TT--C-C-AG-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-T-G-G-A--G-C----C-G--C-G---G--G--------------A--GA-G-A---T---------------G--G-A--TA--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-A--C-----T-GG------AA-G-A--------------G-G-T-A---AG-C--------------GG--A--ATA-----------G-C-T-G-GT--GT-A-G-GG-GT--T--------A-A-A---TC-C-GT-TA--AT-A-TC-----------------------------A-G---C-T--A-G-A-AC-A-CC----AA-A--A--GC-AAA-G--G-C----A-----G--C-T-T-A---CTA-----G--AA-C-A------------------------------------------------------------------------------G-T------T-C-T--GA--CA---CT---------------------------C----A-GG--G-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--TG

Otu0013809 M01867_242_000000000_ATWYL_1_2113_10912_24269 .AGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-C-A---GC-A-----A--C-TCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--C--G-G-CC---C-----TC--G-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TGTTGAG-T--GGGAAG---A--ACCGGT--ATA--TAA-A---T-A-A------------------------------T----------------------------AA--------------------------------G-------------------------------TAT-A-TACTCT-G-A-C-G-----G-T---A-C-CA--------C-T-T-T---------A-G-----------A--AA-------------GC-T--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGA------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GC-GA-AC-------G-TA-G-G-C-G---------------G--G-GA-A-G-T----------------------AA----G-T-T-A------------G-G-A--G--TG--A-AA-TT--T-A-TC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-G-T-A-A--T-C----T-G--C-T---T--T--------------T--AA-T-A---C---------------T--G-C--TT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GG------GA-G-A--------------G-G-A-T---GA-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----AG-T--T--GC-GAA-G--G-C----G-----G--T-C-A-T---CTG-----G--CC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--T-T-CG-A--AA-G-T----T-GG--GG-G--AG-C-G-AA--CA

Otu0013850 M01867_242_000000000_ATWYL_1_2110_20069_17325 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--GA---------------------------GA-T--A--T-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------A-G--A--A--G-G-CA---------GA-AA-------T-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-A-T--A-TGG----G--A-A--G---AA-TAAGGT---T-A-G--T-G--A-------------------G--A----------------------------GA--------------------------------A--------A---------------CG---C-TAG-C-TG-A-T-G-A-C-G-----G-T---A-C-CA--------T-A-T-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AT-------G-TA-G-G-C-G---------------G--T-TA-G-A-T----------------------AA----G-T-G-T------------G-G-A--T--TG--A-AA-GA--C-C-AT-G-G-----------------------------------------------------CT-T-AA-----------C-C-A-T-G-A-G--T-T----T-G--G-T---C--T--------------G--CA-A-A---C---------------T--G-T--CT--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-AG------TA-G-G--------------G-G-T-A---AC-T--------------GG--A--ATT-----------C-C-G-C-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TG-----------------------------C-G---G-A--A-G-A-AT-A-CT----TG-T--G--GC-GAA-G--G-C----G-----A--G-T-T-A---CTG-----G--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-AA--T-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0015202 M01867_242_000000000_ATWYL_1_1109_13079_27191 .GGG--G-A-A--TC-TT--CC-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-G-G-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-A-C-TC--------------------------TG-TC-T-TG-CTAGAATA-A--AGCT-GC--AAGGTGA-A---C-------------------------------A--A---------------------------TCT--------------------------------T---------------------------------GCAGTC---T-G-A-C-T-----G-T---A-T-AG--------C-A-G-G---------A-G-----------G--AA-------------GA-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTG------------------TCA-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GT-------G-TA-G-G-T-G---------------G--G-TA-T-G-T----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TC--G-C-TG-G-G-----------------------------------------------------CT-T-AA-----------C-C-C-A-G-C-A--A-C----T-G--C-A---T--C--------------C--GA-A-A---C---------------T--G-C--GT--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-AG------AA-G-A--------------G-G-A-G---AG-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-T-C-T---CTG-----G--TC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CA---C----------------------------T-G--A-AA--C-G-CG-A--AA-G-C----T-AG--GG-G--AG-C-A-AA--CG

Otu0018174 M01867_242_000000000_ATWYL_1_1109_3298_14216 .TAA--G-A-A--TC-TT--CC-A-C------AA-T-G-C--AC---------------------------GA-A--A--G-T--G-T-G-A-T-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-T--G--GA--------------------------T-G--A--A--G-C-CT---------CT-AG-------G-G-G--------T-G-T--A---AA-C-A-TC--------------------------TT-TT-ATT--T-AGG----A--A-A--A---A----------------------------------------------T----------------------------TT------------------------------------T--------------------------------------T-G-A-T-G-----T-T---A-C-TA--------A-A-T-T---------A-A-----------TA-A--------------GG-G--GC-T---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--AG---C-----------------GG--TA-AT-------AC---GA-AG-GCC------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GT-CT-------G-TA-G-A-C-G---------------G--T-TT-A-G-T----------------------AA----G-T-T-A------------A-T-C--G--TA--A-AA-TC--T-T-CT-A-G-----------------------------------------------------CT-C-AA-----------C-T-A-G-A-A-G--T-T----T-G--C-G---A--T--------------T--AA-T-A---C---------------T--A-T--TA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--T-----C-GG------GG-G-A--------------A-G-C-A---AG-T--------------GG--A--ATT-----------G-G-T-G-GT--GT-A-G-TC-GT--A--------A-T-A---AG-C-GT-TA--AT-A-TC-----------------------------A-C---C-A--G-G-A-AC-A-CC----GA-A--A--GC-GAA-A--G-C----A-----G--C-T-T-G---CTA-----A--AA-C-G------------------------------------------------------------------------------A-T------T-C-T--GA-CGT---T----------------------------G----A-GA--G-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AT--AC

Otu0019281 M01867_242_000000000_ATWYL_1_1103_13933_27143 .CGA--G-A-A--TC-TT--TC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-A-C---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TC-A-C--T-AGG----A--A-G--G---AA--AGC-T---T---T--G-A--A-------------------T--G----------------------------AA--------------------------------T---A----A---------------TT---C-AGT-A-GT-T-T-G-A-C-A-----T-T---A-C-CT--------G-G-A-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-T-T---------------G--A-CA-A-G-A----------------------AA----G-T-G-A------------T-T-C--G--TG--A-AA-TC--C-T-AA-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-T-A-G-A--A-C----T-G--C-G---T--T--------------T--CA-A-A---C---------------T--T-C---T--T-G-T-------------------------------------------------------------------------------------------------------------------------------------------------------------------------CTT-G-A-G-T--A-----T-TG------AA-G-A--------------G-G-T-A---AG-T--------------AG--A--ATT-----------C-T-C-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------G-A---G-A--G-G-A-AT-A-CC----TT-T--G--GT-GAA-G--A-C----G-----G--C-T-T-A---CTA-----T--TC-A-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-GG--C-A-CG-A--AG-G-T----A-TG--GG-G--AG-C-A-AA--CA

Otu0020301 M01867_242_000000000_ATWYL_1_1114_11596_3022 .AAA--G-C-C--TG-AC--GC-A-G------CA-A-C-G--CC---------------------------GC-G--T--G-A--A-T-G-A-T-G-A-A---GT-T-----C--T-TCG-G-A-A-T-----G-T-A--A--AG--------------------------T-T--C--T--T-T-CA---------GT-GG-------G-A-A--------A-G-A--AC--AA-G-T-CCG-------------------------GG-AG-GAA--A-TGC----C--C-G--G---G----------------------------------------------C----------------------------CC---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CC--------A-C-A-G---------A-A-----------G--AA-------------GC-C--CT-G---G-C-TAA-----------------------A--T-A-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-A-T-G---------------G--T-TT-T-G-A----------------------AA----G-T-C-A------------G-A-A--G--TG--A-AA-GC--C-C-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-G-G-A--AT-----T-G--C-T---T--T--------------T--GA-A-A---C---------------T--T-C--AT--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-AG------GA-G-A--------------G-G-A-A---AG-T--------------GG--A--ATT-----------T-C-A-A-GT--GG-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------T-G---G-A--A-G-A-AC-A-CC---TGT----G--GC-GAA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-T-G------------------------------------------------------------------------------C-A------A-C-T--GA--CA---T----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CG

Otu0021846 M01867_242_000000000_ATWYL_1_2102_23653_6840 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-G-----A--T-ACC-G-C-G-T-----G-A-T--C--GA--------------------------T-G--A--A--G-G-TC---------GA-GA-------G-A-T--------T-G-T--A---AA-G-A-TC--------------------------TT-TT-A-C--A-GCT----G--A-G--G---AA-TAAA-T---C-T-G--G-G--A-------------------G--A----------------------------GA--------------------------------A--------T---------------GC---C-CGG-A-TG-A-T-G-A-C-G-----T-T---A-A-GC--------T-G-T-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AA-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AC-------G-CA-G-G-C-G---------------G--C-CC-G-A-C----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-GA--C-C-TG-G-G-----------------------------------------------------CT-T-AA-----------C-T-C-A-G-G-G--A-C----C-G--C-G---T--T--------------G--GA-A-A---C---------------T--G-T--CG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--C-----T-TG------GA-G-G--------------G-G-G-A---GT-C--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-TG--AT-A-TT-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--A-C-T-C---CTG-----G--CC-G-A------------------------------------------------------------------------------A-G------A-T-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-C----A-TA--GG-G--AG-C-A-AA--CA

Otu0022246 M04041_72_000000000_AUMPE_1_2114_20648_18895 .GGG--G-A-A--AC-CC--TG-A-C------GA-C-G-C--GA---------------------------CG-C--C--G-C--G-T-G-A-G-C-G-A---TG-A-----A--G-GCCTT-C-G-G-----GTT-G--T--AA--------------------------A-GC-T--C--T-G-TC---------AC-TA-------G-G-A--------A-G-G--A---AA-G-C-TT-------------------------TGAATGAA-T--A-ATT----C--A-G--T---A----------------------------------------------G----------------------------TT---------------------------------------------------------------------------T-G-A-C-A-----T-T---A-C-CT--------G-G-A-G---------A-G-----------G--AA-------------GC-C--CC-G---G-G-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-T-T---------------G--A-CA-G-G-C----------------------AA----G-T-G-A------------T-T-C--G--TG--A-AA-TC--C-T-AA-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-T-A-G-A--A-C----T-G--C-G---A--T--------------T--CA-A-A---C---------------T--G-C--TT--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-TG------AA-G-A--------------G-G-T-A---AG-T--------------AG--A--ATT-----------C-T-C-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------G-A---G-A--G-G-A-AT-A-CC---TTT----G--GT-GAA-G--A-C----G-----G--C-T-T-A---CTA-----T--TC-A-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-GG--C-A-CG-A--AG-G-C----A-TG--GG-G--AG-C-A-AA--CA

Otu0003550 M01867_242_000000000_ATWYL_1_2103_21336_20053 .TTC--G-A-A--TC-AT--CC-A-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-T-G-G-T---GC-A-----A--C-GCC-G-C-G-T-----G-G-G--G--GA--------------------------T-G--A--A--G-G-TC---------CT-CG-------G-A-T--------T-G-T--A---AA-C-C-CC--------------------------TG-TC-A-C--C-AAG----G--A-A--CA--AA-ATGG-T---A-T-G--A---------------------------T----------------------------GA------------------------------------A-------------------------T-GC--A--G-T-T-G-A-G-T-----T-A---A-C-TT--------G-G-A-G---------A-G-----------G--AA-------------GC-A--GT-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-ACT------------------GCG-A-G------------C--G--T---T--AC-T-CGG-AT------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-AG-G-G-T----------------------GT----G-T-C-A------------G-G-T--G--TG--A-AA-TC--C-C-GG-G-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-G-G-A--A-G----T-G--C-G---C--C--------------T--GA-A-A---C---------------T--A-T--TC--T-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--C-----C-TG------GA-G-G--------------G-G-A-C---AG-C--------------AG--A--ATT-----------T-C-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---A-A--G-G-A-AT-A-CC---AAT----G--GC-GAA-G--G-C----A-----G--C-T-G-T---CTG-----G--AC-A-G------------------------------------------------------------------------------G-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--AG

Otu0023706 M04041_72_000000000_AUMPE_1_2101_15129_14733 .TTC--G-A-A--TC-AT--CC-A-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-T-G-G-T---GC-A-----A--C-GCC-G-C-G-T-----G-G-G--G--GA--------------------------T-G--A--A--G-G-TC---------CT-CG-------G-A-T--------T-G-T--A---AA-C-C-CC--------------------------TG-TC-A-C--C-AAG----G--A-A--C---AA--AAT-G-----G-T--A---------------------------T----------------------------GA--------------------------------T---G-------------------------A-ATG-C-AG-T-T-G-A-G-T-----T-A---A-C-TT--------G-G-A-G---------A-G-----------G--AA-------------GC-A--GT-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-ACT------------------GCG-A-G------------C--G--T---T--AC-T-CGG-AT------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-AG-G-G-T----------------------GT----G-T-C-A------------G-G-T--G--TG--A-AA-TC--C-C-GG-G-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-G-G-A--A-G----T-G--C-G---C--C--------------T--GA-A-A---C---------------T--A-T--TC--T-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--C-----C-TG------GA-G-G--------------G-G-A-C---AG-C--------------AG--A--ATT-----------T-C-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---A-A--G-G-A-AT-A-CC----AA-T--G--GC-GAA-G--G-C----A-----G--C-T-G-T---CTG-----G--AA-A-G------------------------------------------------------------------------------G-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--AG

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Table S3. Summary of all 16S rRNA (gene) OTUs at the approximate species-level (97% identity), which significantly responded (logFC>2, FDR-

corrected p-value <0.05) to N-Acetylglucosamine (GlcNAc) amendment as compared to the initial sediment and the respective timepoint in the no-

substrate incubation. For each OTU the phylogenetic affiliation, the logFC, the average relative abundance (n=3) in the individual treatments and time

points at the 16S rRNA gene and 16S rRNA level as well as the representative sequence is given.

LogFC value of significantly responding OTUs Mean relative abundance (%) - 16S rRNA genes Mean relative abundance (%) - 16S rRNA

16S rRNA genes 16S rRNA with GlcNAc without GlcNAc with GlcNAc without GlcNAc

OTU Phylum Class Order Family Genus 7 d 17 d 27 d 7 d

17 d 27 d

Initial sediment 7 d 17 d 27 d

Initial sediment 7 d 17 d 27 d

Initial sediment 7 d 17 d 27 d

Initial sediment 7 d 17 d 27 d

Otu0002703 Acidobacteria Acidobacteria_Gp22 Gp22 unclassified unclassified - - - - 2.2 - 0.014 0.015 0.006 0.012 0.009 0.007 0.014 0.009 0.002 0.003 0.014 0.007 0.003 0.006 0.001 0.009

Otu0014689 Acidobacteria Holophagae Holophagales Holophagaceae Holophaga - - - - 3.8 - 0.000 0.000 0.003 0.000 0.000 0.000 0.000 0.000 0.000 0.001 0.017 0.002 0.000 0.001 0.001 0.000

Otu0005383 Bacteroidetes Bacteroidia Bacteroidales Bacteroidaceae Anaerorhabdus - - - - - 6.0 0.000 0.000 0.003 0.080 0.000 0.000 0.000 0.000 0.000 0.000 0.003 0.025 0.000 0.000 0.000 0.000

Otu0002701 Bacteroidetes Bacteroidia Bacteroidales Sunxiuqinia unclassified - - 3.3 - - - 0.001 0.009 0.012 0.016 0.001 0.002 0.003 0.001 0.005 0.016 0.026 0.016 0.004 0.007 0.009 0.007

Otu0007976 Bacteroidetes Bacteroidia Bacteroidales Sunxiuqinia unclassified - - 3.4 - 2.7 - 0.000 0.002 0.004 0.009 0.000 0.001 0.001 0.001 0.001 0.001 0.012 0.008 0.000 0.001 0.000 0.002

Otu0000121 Bacteroidetes unclassified unclassified unclassified unclassified - - - 3.6 3.8 3.7 0.103 0.185 0.316 0.380 0.109 0.150 0.151 0.163 0.017 0.208 0.221 0.213 0.017 0.049 0.035 0.047

Otu0000312 Bacteroidetes unclassified unclassified unclassified unclassified - - - 3.1 3.2 - 0.059 0.078 0.118 0.151 0.064 0.069 0.076 0.083 0.009 0.079 0.084 0.078 0.009 0.018 0.016 0.026

Otu0000704 Bacteroidetes unclassified unclassified unclassified unclassified 2.2 5.5 7.0 3.6 6.2 7.1 0.002 0.011 0.112 0.323 0.002 0.002 0.002 0.002 0.001 0.024 0.153 0.289 0.001 0.004 0.003 0.003

Otu0001780 Bacteroidetes unclassified unclassified unclassified unclassified - 2.3 - 3.2 3.4 3.4 0.004 0.015 0.022 0.023 0.005 0.009 0.003 0.008 0.001 0.022 0.025 0.025 0.001 0.003 0.005 0.004

Otu0001871 Bacteroidetes unclassified unclassified unclassified unclassified - 2.1 - - 2.7 2.2 0.002 0.006 0.012 0.007 0.001 0.003 0.003 0.002 0.005 0.017 0.036 0.025 0.010 0.006 0.008 0.003

Otu0002106 Bacteroidetes unclassified unclassified unclassified unclassified - - - - - 3.8 0.002 0.008 0.014 0.009 0.016 0.007 0.004 0.003 0.001 0.047 0.049 0.034 0.035 0.018 0.016 0.006

Otu0002159 Bacteroidetes unclassified unclassified unclassified unclassified - - - - 4.0 - 0.000 0.000 0.004 0.000 0.000 0.000 0.001 0.000 0.000 0.002 0.019 0.001 0.000 0.002 0.001 0.000

Otu0002784 Bacteroidetes unclassified unclassified unclassified unclassified - - 2.8 2.5 2.7 2.9 0.002 0.004 0.015 0.018 0.002 0.004 0.004 0.002 0.002 0.016 0.018 0.020 0.001 0.003 0.002 0.002

Otu0003018 Bacteroidetes unclassified unclassified unclassified unclassified - - - - 2.2 - 0.007 0.008 0.011 0.014 0.006 0.008 0.007 0.009 0.001 0.004 0.010 0.004 0.001 0.001 0.001 0.002

Otu0004457 Bacteroidetes unclassified unclassified unclassified unclassified - - - - - 2.1 0.000 0.000 0.002 0.003 0.001 0.000 0.001 0.001 0.001 0.005 0.007 0.009 0.004 0.003 0.004 0.001

Otu0006442 Bacteroidetes unclassified unclassified unclassified unclassified - 3.0 4.3 - 3.6 4.4 0.000 0.000 0.007 0.019 0.000 0.000 0.000 0.000 0.000 0.000 0.015 0.026 0.000 0.000 0.000 0.000

Otu0002933 Candidatus Saccharibacteria unclassified unclassified unclassified unclassified - - 7.7 - - 4.7 0.000 0.000 0.001 0.209 0.000 0.000 0.000 0.000 0.000 0.000 0.001 0.032 0.000 0.000 0.000 0.000

Otu0000209 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - - - 2.8 3.8 0.150 0.114 0.159 0.219 0.137 0.121 0.117 0.106 0.008 0.022 0.058 0.116 0.003 0.013 0.006 0.014

Otu0001154 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - 2.5 3.1 - - 3.2 0.001 0.001 0.012 0.017 0.000 0.001 0.000 0.001 0.000 0.002 0.007 0.014 0.000 0.000 0.001 0.000

Otu0001284 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - 3.7 - - - 0.002 0.001 0.016 0.038 0.009 0.008 0.019 0.003 0.002 0.004 0.002 0.008 0.001 0.005 0.007 0.003

Otu0002560 Chloroflexi Anaerolineae Anaerolineales Anaerolineaceae unclassified - - - - 2.1 - 0.012 0.007 0.008 0.009 0.012 0.011 0.008 0.009 0.000 0.002 0.006 0.004 0.002 0.003 0.000 0.003

Otu0000433 Cyanobacteria/Chloroplast Cyanobacteria Family_II GpIIa unclassified - - - - 2.6 - 0.002 0.036 0.023 0.020 0.028 0.021 0.020 0.030 0.012 0.006 0.080 0.005 0.007 0.011 0.007 0.018

Otu0001438 Cyanobacteria/Chloroplast Cyanobacteria Family_II GpIIa unclassified - - - - - 3.2 0.042 0.017 0.037 0.002 0.023 0.047 0.040 0.024 0.003 0.005 0.002 0.033 0.002 0.005 0.001 0.002

Otu0000115 Cyanobacteria/Chloroplast Cyanobacteria unclassified unclassified unclassified - - - - - 6.1 0.001 0.003 0.037 0.001 0.003 0.002 0.001 0.000 0.003 0.005 0.133 0.289 0.006 0.006 0.098 0.003

Otu0003542 Cyanobacteria/Chloroplast Cyanobacteria unclassified unclassified unclassified - - - 3.4 - - 0.001 0.001 0.001 0.002 0.001 0.001 0.002 0.001 0.001 0.026 0.002 0.002 0.001 0.004 0.003 0.001

Otu0002196 Firmicutes Clostridia Clostridiales Clostridiales_unclassified unclassified - - 4.6 - - - 0.001 0.020 0.038 0.037 0.000 0.017 0.015 0.008 0.000 0.009 0.009 0.010 0.000 0.011 0.008 0.004

Otu0000341 Firmicutes Clostridia Clostridiales Ruminococcaceae Saccharofermentans 3.1 6.3 8.6 - 4.7 7.2 0.002 0.025 0.245 1.169 0.001 0.005 0.005 0.005 0.000 0.007 0.045 0.251 0.000 0.003 0.001 0.002

Otu0000349 Firmicutes Clostridia Clostridiales Ruminococcaceae Saccharofermentans - 4.6 6.5 - 3.7 5.2 0.004 0.053 0.113 0.406 0.004 0.018 0.016 0.011 0.001 0.013 0.027 0.077 0.003 0.005 0.003 0.006

Otu0002800 Firmicutes Clostridia Clostridiales Ruminococcaceae Saccharofermentans - - 3.8 - - - 0.000 0.000 0.001 0.013 0.000 0.000 0.000 0.000 0.000 0.000 0.001 0.004 0.000 0.001 0.000 0.000

Otu0009600 Firmicutes Clostridia Clostridiales Ruminococcaceae unclassified - - - - - 3.0 0.000 0.000 0.000 0.014 0.000 0.000 0.001 0.002 0.000 0.000 0.000 0.013 0.000 0.001 0.001 0.002

Otu0011033 Firmicutes Clostridia Clostridiales Syntrophomonadaceae Syntrophomonas - - 2.3 - - - 0.001 0.001 0.003 0.008 0.001 0.001 0.001 0.001 0.000 0.000 0.000 0.001 0.000 0.000 0.000 0.000

Otu0000389 Firmicutes Clostridia Clostridiales unclassified unclassified 5.9 6.4 6.0 4.7 5.1 5.1 0.003 0.206 0.293 0.212 0.002 0.032 0.029 0.025 0.001 0.056 0.072 0.070 0.000 0.013 0.007 0.007

Otu0001632 Firmicutes Clostridia Clostridiales unclassified unclassified - 6.0 5.9 2.5 - 3.7 0.000 0.027 0.065 0.058 0.001 0.016 0.015 0.010 0.000 0.007 0.015 0.016 0.000 0.004 0.004 0.003

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Otu0002104 Firmicutes Clostridia Clostridiales unclassified unclassified - 5.7 5.8 - 3.1 3.1 0.000 0.025 0.066 0.073 0.000 0.010 0.011 0.006 0.001 0.003 0.016 0.017 0.000 0.003 0.001 0.002

Otu0003707 Firmicutes Clostridia Clostridiales unclassified unclassified - 3.1 3.4 - - - 0.000 0.003 0.008 0.010 0.000 0.001 0.000 0.001 0.000 0.002 0.003 0.005 0.000 0.001 0.000 0.000

Otu0006234 Firmicutes Clostridia Clostridiales unclassified unclassified - 4.8 3.5 - - - 0.000 0.012 0.028 0.011 0.000 0.004 0.002 0.000 0.000 0.000 0.001 0.002 0.000 0.000 0.000 0.000

Otu0001463 Firmicutes Negativicutes Selenomonadales Veillonellaceae unclassified 5.9 6.1 5.3 4.4 4.1 3.1 0.000 0.062 0.072 0.038 0.001 0.013 0.015 0.010 0.001 0.055 0.042 0.020 0.000 0.009 0.005 0.001

Otu0004716 Firmicutes Negativicutes Selenomonadales Veillonellaceae unclassified - 3.2 3.7 - - 3.5 0.001 0.004 0.016 0.023 0.000 0.005 0.002 0.003 0.000 0.001 0.005 0.014 0.001 0.002 0.000 0.002

Otu0006138 Firmicutes Negativicutes Selenomonadales Veillonellaceae unclassified - - 3.9 - - - 0.000 0.001 0.007 0.014 0.000 0.001 0.002 0.003 0.000 0.001 0.001 0.003 0.000 0.003 0.000 0.001

Otu0006141 Firmicutes Negativicutes Selenomonadales Veillonellaceae unclassified 3.5 - 3.1 - - - 0.000 0.015 0.016 0.010 0.000 0.002 0.003 0.000 0.000 0.009 0.006 0.006 0.000 0.002 0.001 0.001

Otu0011769 Firmicutes Negativicutes Selenomonadales Veillonellaceae unclassified - - 2.9 - - - 0.000 0.000 0.004 0.006 0.000 0.001 0.000 0.000 0.000 0.000 0.002 0.001 0.000 0.000 0.000 0.000

Otu0000139 Fusobacteria Fusobacteriia Fusobacteriales Fusobacteriaceae unclassified 8.0 6.1 - 7.6 5.8 - 0.001 0.512 0.138 0.014 0.002 0.006 0.008 0.003 0.004 0.967 0.267 0.022 0.005 0.025 0.008 0.005

Otu0000344 Fusobacteria Fusobacteriia Fusobacteriales Fusobacteriaceae unclassified 8.2 7.0 4.9 8.1 6.1 - 0.000 0.384 0.165 0.038 0.001 0.010 0.005 0.003 0.001 0.613 0.147 0.024 0.000 0.013 0.005 0.007

Otu0009344 Fusobacteria Fusobacteriia Fusobacteriales Fusobacteriaceae unclassified - - - 3.1 - - 0.000 0.005 0.001 0.000 0.000 0.000 0.000 0.000 0.000 0.011 0.001 0.000 0.000 0.001 0.000 0.000

Otu0003625 Lentisphaerae Lentisphaeria Victivallales Victivallaceae Victivallis - 3.3 4.5 - 3.7 4.2 0.001 0.004 0.015 0.033 0.001 0.002 0.001 0.000 0.001 0.004 0.028 0.037 0.000 0.002 0.001 0.001

Otu0002416 Lentisphaerae Oligosphaeria Oligosphaerales Oligosphaeraceae Oligosphaera - - 2.6 - - 3.6 0.006 0.004 0.010 0.038 0.007 0.004 0.005 0.005 0.004 0.003 0.015 0.062 0.005 0.001 0.005 0.006

Otu0004551 Parcubacteria unclassified unclassified unclassified unclassified - 2.5 4.4 - - 3.1 0.001 0.001 0.010 0.038 0.000 0.001 0.001 0.001 0.000 0.001 0.001 0.014 0.000 0.000 0.001 0.001

Otu0002854 Planctomycetes Planctomycetia Planctomycetales Planctomycetaceae unclassified 2.1 - - - - - 0.003 0.016 0.010 0.003 0.008 0.003 0.009 0.006 0.002 0.003 0.001 0.001 0.001 0.001 0.001 0.003

Otu0013998 Planctomycetes Planctomycetia Planctomycetales Planctomycetaceae unclassified - - 2.6 - - - 0.000 0.001 0.001 0.005 0.000 0.001 0.002 0.000 0.001 0.001 0.000 0.002 0.000 0.000 0.001 0.000

Otu0000085 Proteobacteria Alphaproteobacteria Rhodospirillales Rhodospirillaceae Azospirillum 5.0 8.6 9.6 4.8 8.1 8.7 0.000 0.042 0.529 1.013 0.000 0.000 0.000 0.000 0.007 0.234 2.059 3.328 0.000 0.000 0.002 0.006

Otu0001917 Proteobacteria Alphaproteobacteria Rhodospirillales Rhodospirillaceae Azospirillum 4.0 4.5 3.8 4.6 5.0 4.5 0.001 0.025 0.037 0.021 0.001 0.000 0.001 0.000 0.002 0.073 0.095 0.064 0.001 0.003 0.001 0.002

Otu0003281 Proteobacteria Alphaproteobacteria Rhodospirillales Rhodospirillaceae Azospirillum 3.6 3.8 - 5.0 5.1 - 0.000 0.015 0.017 0.002 0.000 0.001 0.002 0.001 0.001 0.070 0.075 0.009 0.000 0.001 0.003 0.003

Otu0006228 Proteobacteria Alphaproteobacteria Rhodospirillales Rhodospirillaceae Azospirillum 2.6 - - 3.4 4.4 - 0.000 0.007 0.008 0.000 0.001 0.000 0.000 0.000 0.001 0.023 0.041 0.001 0.000 0.001 0.001 0.001

Otu0016688 Proteobacteria Alphaproteobacteria Rhodospirillales Rhodospirillaceae Azospirillum - - - - 2.9 - 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.011 0.000 0.000 0.000 0.000

Otu0027482 Proteobacteria Alphaproteobacteria Rhodospirillales Rhodospirillaceae Azospirillum - - - - - 2.6 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.001 0.007 0.000 0.000 0.000 0.000

Otu0032692 Proteobacteria Alphaproteobacteria Rhodospirillales Rhodospirillaceae Azospirillum - - - - - 2.4 0.000 0.000 0.000 0.001 0.000 0.000 0.000 0.000 0.000 0.000 0.001 0.006 0.000 0.000 0.000 0.000

Otu0001918 Proteobacteria Alphaproteobacteria Rhodospirillales Rhodospirillaceae Insolitispirillum 2.7 - - 3.4 2.5 - 0.004 0.027 0.008 0.003 0.003 0.003 0.002 0.011 0.001 0.022 0.010 0.002 0.003 0.003 0.002 0.002

Otu0000755 Proteobacteria Alphaproteobacteria Rhodospirillales Rhodospirillaceae unclassified 3.8 5.1 - 6.2 7.2 4.0 0.001 0.027 0.064 0.004 0.001 0.000 0.001 0.000 0.002 0.216 0.415 0.046 0.001 0.002 0.008 0.004

Otu0000364 Proteobacteria Alphaproteobacteria unclassified unclassified unclassified - 8.9 8.8 3.3 9.0 8.3 0.000 0.004 0.632 0.574 0.000 0.000 0.000 0.000 0.001 0.020 1.113 0.678 0.000 0.000 0.000 0.000

Otu0002590 Proteobacteria Alphaproteobacteria unclassified unclassified unclassified - 6.8 5.4 - 6.7 4.9 0.000 0.003 0.117 0.042 0.000 0.000 0.000 0.000 0.000 0.009 0.184 0.050 0.000 0.000 0.000 0.000

Otu0010233 Proteobacteria Alphaproteobacteria unclassified unclassified unclassified - 4.0 3.7 - 4.3 3.1 0.000 0.000 0.015 0.012 0.000 0.000 0.000 0.000 0.000 0.000 0.026 0.010 0.000 0.000 0.000 0.000

Otu0021324 Proteobacteria Alphaproteobacteria unclassified unclassified unclassified - - - - - 2.9 0.000 0.000 0.002 0.002 0.000 0.000 0.000 0.000 0.000 0.000 0.006 0.009 0.000 0.000 0.000 0.000

Otu0000486 Proteobacteria Betaproteobacteria Burkholderiales unclassified unclassified - 2.3 - - - - 0.003 0.006 0.018 0.016 0.006 0.007 0.003 0.007 0.036 0.032 0.039 0.033 0.049 0.029 0.036 0.037

Otu0000911 Proteobacteria Betaproteobacteria Burkholderiales unclassified unclassified - - 2.7 - - - 0.004 0.018 0.018 0.029 0.003 0.004 0.015 0.005 0.011 0.055 0.005 0.005 0.008 0.006 0.008 0.005

Otu0002277 Proteobacteria Betaproteobacteria Hydrogenophilales Hydrogenophilaceae Thiobacillus - - - - 3.2 - 0.002 0.001 0.001 0.003 0.001 0.002 0.001 0.023 0.004 0.005 0.044 0.023 0.003 0.005 0.005 0.005

Otu0000498 Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae Chitinilyticum 6.2 8.1 7.5 - 7.0 6.7 0.000 0.076 0.285 0.175 0.000 0.005 0.011 0.002 0.001 0.050 0.272 0.214 0.000 0.010 0.017 0.011

Otu0000685 Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae Chitinilyticum 5.0 6.7 6.4 4.7 6.5 6.5 0.001 0.051 0.168 0.135 0.000 0.004 0.004 0.002 0.000 0.033 0.123 0.120 0.000 0.007 0.006 0.006

Otu0004569 Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae Chitinilyticum 3.7 4.6 4.2 2.9 4.1 - 0.000 0.012 0.025 0.017 0.000 0.000 0.000 0.000 0.000 0.009 0.021 0.016 0.000 0.000 0.000 0.000

Otu0011993 Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae Deefgea - 2.2 - - - - 0.000 0.004 0.005 0.006 0.000 0.000 0.000 0.000 0.000 0.002 0.004 0.004 0.000 0.000 0.000 0.000

Otu0000582 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Propionivibrio - 8.4 8.9 - - 9.0 0.000 0.088 0.355 0.459 0.000 0.000 0.000 0.001 0.000 0.055 0.205 0.682 0.000 0.002 0.000 0.002

Otu0000669 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Propionivibrio - 2.8 - 4.7 5.8 - 0.001 0.048 0.012 0.009 0.003 0.003 0.001 0.003 0.006 0.173 0.364 0.011 0.006 0.008 0.011 0.005

Otu0000805 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Propionivibrio 4.0 6.3 7.7 4.0 7.2 8.2 0.000 0.020 0.104 0.270 0.000 0.000 0.001 0.000 0.001 0.033 0.297 0.623 0.000 0.000 0.000 0.001

Otu0001987 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Propionivibrio - 3.3 4.1 - - 3.5 0.002 0.010 0.023 0.040 0.003 0.003 0.005 0.003 0.005 0.020 0.043 0.065 0.007 0.010 0.013 0.009

Otu0004515 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Propionivibrio 2.3 3.7 4.2 3.2 4.4 4.8 0.001 0.007 0.021 0.028 0.000 0.001 0.000 0.000 0.000 0.012 0.026 0.035 0.000 0.001 0.000 0.001

Otu0007375 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Propionivibrio - - 2.5 - - 3.8 0.001 0.002 0.007 0.011 0.001 0.000 0.001 0.000 0.000 0.004 0.015 0.017 0.000 0.004 0.002 0.001

Otu0010360 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Propionivibrio - - - - 3.4 3.5 0.000 0.001 0.001 0.001 0.000 0.001 0.000 0.000 0.000 0.000 0.012 0.014 0.001 0.001 0.000 0.000

Otu0012224 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Propionivibrio - - 3.4 - 2.8 4.1 0.000 0.000 0.003 0.010 0.000 0.000 0.000 0.000 0.000 0.000 0.007 0.022 0.000 0.000 0.000 0.000

Otu0013357 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Propionivibrio - - 2.5 - - 3.4 0.000 0.001 0.003 0.006 0.000 0.000 0.000 0.000 0.000 0.001 0.007 0.012 0.000 0.000 0.000 0.000

Otu0015964 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Propionivibrio - - - - - 2.6 0.000 0.002 0.003 0.003 0.000 0.000 0.000 0.000 0.000 0.002 0.005 0.007 0.000 0.000 0.000 0.000

Otu0019359 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Propionivibrio - - 2.5 - 2.5 3.0 0.000 0.000 0.002 0.005 0.000 0.000 0.000 0.000 0.000 0.000 0.006 0.009 0.000 0.000 0.000 0.000

Otu0022389 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Propionivibrio - - - - - 3.0 0.000 0.000 0.001 0.003 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.009 0.000 0.000 0.000 0.000

Otu0000050 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Uliginosibacterium 7.7 7.5 7.1 8.3 8.6 7.5 0.004 0.961 0.861 0.628 0.000 0.003 0.005 0.004 0.007 2.538 2.904 1.364 0.007 0.014 0.010 0.004

Otu0002659 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Uliginosibacterium 4.8 4.8 4.2 4.9 5.4 4.6 0.000 0.029 0.026 0.017 0.000 0.000 0.000 0.001 0.001 0.053 0.073 0.041 0.000 0.000 0.000 0.001

Otu0018965 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Uliginosibacterium - - - 2.8 - - 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.001 0.000 0.001 0.007 0.000 0.000 0.000 0.000

Otu0019141 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Uliginosibacterium - - - 2.2 - - 0.000 0.000 0.001 0.001 0.000 0.000 0.000 0.000 0.000 0.005 0.005 0.004 0.000 0.000 0.000 0.000

Otu0029503 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Uliginosibacterium - - - - 2.5 - 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.002 0.006 0.001 0.000 0.000 0.000 0.000

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Otu0000066 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae unclassified 7.0 8.9 9.4 4.9 7.6 8.1 0.001 0.214 0.810 1.089 0.000 0.000 0.000 0.003 0.008 0.245 1.551 2.280 0.002 0.002 0.003 0.008

Otu0001812 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae unclassified - 5.1 6.0 2.8 5.4 5.8 0.000 0.001 0.035 0.063 0.000 0.000 0.000 0.000 0.000 0.002 0.053 0.072 0.000 0.000 0.000 0.000

Otu0004429 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae unclassified - - 2.7 - - - 0.001 0.004 0.010 0.011 0.001 0.003 0.002 0.002 0.003 0.007 0.010 0.016 0.005 0.003 0.006 0.004

Otu0004850 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae unclassified - 3.7 3.7 - 3.1 3.1 0.000 0.009 0.016 0.016 0.001 0.004 0.001 0.001 0.002 0.008 0.024 0.023 0.003 0.003 0.003 0.003

Otu0005045 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae unclassified - 4.0 4.5 - 3.7 3.7 0.000 0.002 0.020 0.029 0.001 0.001 0.003 0.001 0.000 0.002 0.020 0.022 0.001 0.003 0.001 0.001

Otu0011887 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae unclassified - - 2.9 - - - 0.000 0.001 0.005 0.006 0.000 0.000 0.001 0.000 0.001 0.000 0.001 0.003 0.000 0.001 0.001 0.000

Otu0014966 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae unclassified - - - - - 2.7 0.000 0.001 0.001 0.003 0.000 0.000 0.001 0.000 0.000 0.000 0.004 0.008 0.001 0.000 0.000 0.000

Otu0014976 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae unclassified - - - - 3.4 3.2 0.000 0.000 0.000 0.001 0.000 0.000 0.000 0.000 0.000 0.000 0.013 0.011 0.000 0.000 0.000 0.000

Otu0020270 Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae unclassified - - - - - 2.7 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.001 0.001 0.008 0.000 0.000 0.000 0.000

Otu0001025 Proteobacteria Betaproteobacteria unclassified unclassified unclassified - - 3.1 - - - 0.004 0.051 0.026 0.035 0.042 0.019 0.053 0.004 0.023 0.024 0.019 0.012 0.001 0.044 0.017 0.032

Otu0006109 Proteobacteria Betaproteobacteria unclassified unclassified unclassified - 5.0 5.2 - 4.5 4.0 0.000 0.000 0.031 0.035 0.000 0.000 0.001 0.001 0.000 0.001 0.027 0.019 0.001 0.001 0.001 0.000

Otu0007481 Proteobacteria Betaproteobacteria unclassified unclassified unclassified - 4.1 - - - - 0.000 0.005 0.017 0.005 0.001 0.005 0.004 0.002 0.000 0.002 0.009 0.001 0.000 0.001 0.003 0.001

Otu0008702 Proteobacteria Betaproteobacteria unclassified unclassified unclassified - 3.6 - - 3.8 - 0.000 0.002 0.012 0.003 0.000 0.003 0.002 0.001 0.000 0.003 0.017 0.002 0.000 0.003 0.002 0.002

Otu0004178 Proteobacteria Deltaproteobacteria Bdellovibrionales Bdellovibrionaceae Vampirovibrio - 6.1 4.0 - 6.1 3.3 0.000 0.000 0.070 0.015 0.000 0.000 0.000 0.000 0.001 0.001 0.117 0.015 0.000 0.000 0.000 0.000

Otu0010716 Proteobacteria Deltaproteobacteria Bdellovibrionales Bdellovibrionaceae Vampirovibrio - - 3.3 - 3.3 4.5 0.000 0.000 0.005 0.012 0.000 0.000 0.000 0.000 0.000 0.001 0.011 0.030 0.000 0.000 0.000 0.000

Otu0001198 Proteobacteria Deltaproteobacteria Desulfuromonadales Geobacteraceae Geobacter - - 4.2 - - - 0.002 0.020 0.033 0.049 0.003 0.008 0.011 0.010 0.004 0.040 0.041 0.037 0.002 0.019 0.019 0.015

Otu0001531 Proteobacteria Deltaproteobacteria Desulfuromonadales Geobacteraceae Geobacter 4.6 5.7 5.6 - 4.7 4.9 0.001 0.046 0.096 0.088 0.001 0.010 0.007 0.003 0.001 0.015 0.055 0.060 0.003 0.010 0.004 0.001

Otu0001585 Proteobacteria Deltaproteobacteria Desulfuromonadales Geobacteraceae Geobacter - 3.2 3.6 - - - 0.001 0.007 0.014 0.018 0.002 0.001 0.003 0.004 0.002 0.018 0.033 0.041 0.001 0.014 0.019 0.017

Otu0002112 Proteobacteria Deltaproteobacteria Desulfuromonadales Geobacteraceae Geobacter - - 4.2 - - 4.9 0.000 0.009 0.016 0.022 0.001 0.005 0.005 0.003 0.000 0.012 0.025 0.038 0.000 0.013 0.009 0.010

Otu0002714 Proteobacteria Deltaproteobacteria Desulfuromonadales Geobacteraceae Geobacter 3.4 4.5 4.7 - 3.1 4.4 0.001 0.017 0.036 0.039 0.001 0.002 0.001 0.002 0.002 0.007 0.024 0.060 0.001 0.005 0.005 0.003

Otu0002949 Proteobacteria Deltaproteobacteria Desulfuromonadales Geobacteraceae Geobacter 2.9 4.2 4.7 - 4.4 5.0 0.002 0.018 0.044 0.065 0.001 0.003 0.003 0.002 0.000 0.010 0.027 0.041 0.001 0.005 0.002 0.002

Otu0004964 Proteobacteria Deltaproteobacteria Desulfuromonadales Geobacteraceae Geobacter - 3.1 - - - - 0.000 0.002 0.007 0.002 0.000 0.003 0.001 0.001 0.000 0.004 0.009 0.006 0.000 0.004 0.006 0.002

Otu0006925 Proteobacteria Deltaproteobacteria Desulfuromonadales Geobacteraceae Geobacter - - 2.7 - - - 0.000 0.001 0.003 0.005 0.000 0.001 0.000 0.000 0.000 0.001 0.001 0.003 0.000 0.000 0.000 0.000

Otu0007856 Proteobacteria Deltaproteobacteria Myxococcales unclassified unclassified - - - - - 3.1 0.001 0.001 0.000 0.002 0.000 0.002 0.000 0.000 0.001 0.001 0.006 0.013 0.001 0.002 0.001 0.000

Otu0005126 Proteobacteria Deltaproteobacteria unclassified unclassified unclassified - - - - 3.9 5.5 0.000 0.000 0.003 0.004 0.000 0.000 0.000 0.000 0.000 0.008 0.018 0.057 0.002 0.002 0.002 0.002

Otu0007770 Proteobacteria Deltaproteobacteria unclassified unclassified unclassified - - - - 2.6 2.7 0.000 0.001 0.004 0.005 0.000 0.000 0.000 0.000 0.002 0.003 0.019 0.021 0.000 0.001 0.000 0.000

Otu0009452 Proteobacteria Deltaproteobacteria unclassified unclassified unclassified - - - - - 2.6 0.000 0.001 0.001 0.001 0.000 0.000 0.000 0.000 0.001 0.001 0.008 0.011 0.001 0.001 0.000 0.001

Otu0009567 Proteobacteria Deltaproteobacteria unclassified unclassified unclassified - - - - - 2.2 0.000 0.000 0.001 0.001 0.000 0.000 0.000 0.000 0.002 0.002 0.007 0.012 0.001 0.002 0.002 0.001

Otu0010173 Proteobacteria Deltaproteobacteria unclassified unclassified unclassified - - - - - 2.4 0.000 0.000 0.002 0.002 0.000 0.000 0.000 0.000 0.001 0.002 0.007 0.012 0.001 0.001 0.001 0.001

Otu0012797 Proteobacteria Deltaproteobacteria unclassified unclassified unclassified - - - - 2.4 - 0.000 0.000 0.000 0.001 0.000 0.000 0.000 0.000 0.000 0.000 0.007 0.004 0.000 0.000 0.000 0.000

Otu0015073 Proteobacteria Deltaproteobacteria unclassified unclassified unclassified - - - - - 2.5 0.000 0.001 0.001 0.002 0.000 0.000 0.000 0.000 0.000 0.002 0.007 0.008 0.001 0.000 0.000 0.000

Otu0017042 Proteobacteria Deltaproteobacteria unclassified unclassified unclassified - - - - - 2.8 0.000 0.000 0.000 0.001 0.000 0.000 0.000 0.000 0.001 0.001 0.003 0.014 0.001 0.000 0.000 0.000

Otu0000567 Proteobacteria Epsilonproteobacteria Campylobacterales Campylobacteraceae Sulfurospirillum - 5.2 5.5 - - - 0.002 0.022 0.102 0.123 0.003 0.021 0.019 0.016 0.000 0.007 0.027 0.019 0.000 0.008 0.009 0.006

Otu0001841 Proteobacteria Gammaproteobacteria Aeromonadales Aeromonadaceae Aeromonas 5.7 6.1 5.1 - - - 0.000 0.052 0.072 0.034 0.000 0.010 0.004 0.002 0.000 0.008 0.009 0.004 0.000 0.001 0.000 0.000

Otu0003479 Proteobacteria Gammaproteobacteria Aeromonadales Aeromonadaceae Aeromonas 5.0 6.4 6.2 - 4.3 4.0 0.000 0.032 0.087 0.073 0.000 0.000 0.001 0.000 0.000 0.006 0.023 0.020 0.000 0.001 0.000 0.000

Otu0006024 Proteobacteria Gammaproteobacteria Aeromonadales Aeromonadaceae Aeromonas - 4.4 4.3 - - - 0.000 0.005 0.020 0.019 0.000 0.000 0.000 0.000 0.000 0.002 0.006 0.005 0.000 0.000 0.000 0.000

Otu0008654 Proteobacteria Gammaproteobacteria Aeromonadales Aeromonadaceae Aeromonas - 3.7 2.6 - - - 0.000 0.011 0.016 0.006 0.001 0.003 0.002 0.000 0.000 0.001 0.003 0.000 0.000 0.001 0.000 0.000

Otu0005855 Proteobacteria Gammaproteobacteria Aeromonadales Aeromonadaceae unclassified 3.8 5.0 5.5 - - 3.8 0.000 0.014 0.031 0.044 0.000 0.001 0.000 0.000 0.000 0.002 0.008 0.017 0.000 0.000 0.000 0.000

Otu0000948 Proteobacteria Gammaproteobacteria unclassified unclassified unclassified 6.7 8.1 7.4 - 5.3 - 0.000 0.138 0.377 0.216 0.000 0.001 0.001 0.000 0.000 0.022 0.050 0.030 0.000 0.000 0.000 0.000

Otu0000959 Proteobacteria Gammaproteobacteria unclassified unclassified unclassified 5.2 6.5 6.3 2.7 4.2 4.6 0.001 0.061 0.144 0.124 0.001 0.005 0.005 0.001 0.001 0.011 0.032 0.041 0.000 0.002 0.002 0.000

Otu0002822 Proteobacteria Gammaproteobacteria unclassified unclassified unclassified 4.9 6.4 6.5 2.5 3.6 3.9 0.000 0.031 0.087 0.090 0.000 0.003 0.001 0.001 0.000 0.006 0.014 0.019 0.000 0.001 0.000 0.000

Otu0003724 Proteobacteria Gammaproteobacteria unclassified unclassified unclassified 4.1 5.9 6.2 - - 3.6 0.000 0.017 0.061 0.071 0.000 0.001 0.001 0.001 0.000 0.003 0.015 0.015 0.000 0.001 0.001 0.001

Otu0013041 Proteobacteria Gammaproteobacteria unclassified unclassified unclassified - - 3.0 - - - 0.000 0.004 0.009 0.007 0.000 0.000 0.000 0.000 0.000 0.000 0.002 0.001 0.000 0.000 0.000 0.000

Otu0007037 Proteobacteria unclassified unclassified unclassified unclassified - - - - - 3.4 0.000 0.000 0.000 0.005 0.000 0.000 0.000 0.000 0.002 0.001 0.012 0.031 0.000 0.002 0.003 0.003

Otu0009443 Proteobacteria unclassified unclassified unclassified unclassified - - - - - 2.8 0.000 0.000 0.000 0.002 0.000 0.000 0.000 0.000 0.000 0.002 0.004 0.010 0.000 0.001 0.000 0.000

Otu0010147 Proteobacteria unclassified unclassified unclassified unclassified - - - - - 2.8 0.001 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.002 0.002 0.008 0.019 0.001 0.002 0.000 0.001

Otu0010682 Proteobacteria unclassified unclassified unclassified unclassified - - 2.7 - 2.4 3.2 0.001 0.000 0.002 0.011 0.000 0.000 0.000 0.000 0.001 0.001 0.010 0.018 0.000 0.001 0.000 0.000

Otu0011313 Proteobacteria unclassified unclassified unclassified unclassified - - - - - 2.2 0.002 0.000 0.000 0.002 0.000 0.000 0.000 0.000 0.001 0.001 0.006 0.010 0.002 0.002 0.003 0.001

Otu0013030 Proteobacteria unclassified unclassified unclassified unclassified - - 3.0 - - - 0.000 0.000 0.000 0.007 0.000 0.001 0.001 0.000 0.000 0.001 0.001 0.015 0.001 0.000 0.000 0.001

Otu0013361 Proteobacteria unclassified unclassified unclassified unclassified - - - - 2.8 2.5 0.000 0.000 0.000 0.001 0.001 0.000 0.000 0.000 0.001 0.000 0.013 0.010 0.001 0.000 0.000 0.000

Otu0013605 Proteobacteria unclassified unclassified unclassified unclassified - - - - 2.5 3.4 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.002 0.006 0.013 0.000 0.001 0.000 0.000

Otu0003768 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae unclassified - 3.1 - 3.7 - 2.6 0.001 0.007 0.012 0.004 0.000 0.000 0.000 0.000 0.001 0.027 0.017 0.012 0.001 0.001 0.001 0.000

Otu0004440 Spirochaetes Spirochaetia Spirochaetales Spirochaetaceae unclassified - 3.7 3.7 3.0 3.9 3.6 0.000 0.006 0.012 0.012 0.001 0.001 0.000 0.000 0.000 0.009 0.019 0.014 0.001 0.000 0.001 0.000

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Otu0002856 unclassified unclassified unclassified unclassified unclassified - - - - - 2.5 0.002 0.003 0.003 0.003 0.005 0.013 0.002 0.006 0.003 0.023 0.011 0.008 0.008 0.001 0.022 0.002

Otu0000683 unclassified unclassified unclassified unclassified unclassified - 4.9 4.0 - 4.4 3.5 0.000 0.012 0.030 0.015 0.000 0.001 0.003 0.000 0.000 0.014 0.034 0.019 0.000 0.001 0.003 0.001

Otu0000961 unclassified unclassified unclassified unclassified unclassified - 5.9 - - 6.7 - 0.000 0.001 0.061 0.005 0.000 0.000 0.000 0.001 0.000 0.010 0.135 0.031 0.000 0.000 0.000 0.000

Otu0001026 unclassified unclassified unclassified unclassified unclassified - - 3.0 - 2.5 3.7 0.003 0.010 0.018 0.028 0.005 0.004 0.005 0.002 0.011 0.028 0.061 0.148 0.010 0.018 0.015 0.018

Otu0003286 unclassified unclassified unclassified unclassified unclassified - - - - - 2.8 0.001 0.001 0.008 0.011 0.003 0.003 0.004 0.005 0.001 0.002 0.007 0.014 0.001 0.004 0.003 0.002

Otu0003382 unclassified unclassified unclassified unclassified unclassified - - - - - 2.9 0.001 0.003 0.003 0.004 0.002 0.002 0.003 0.004 0.003 0.009 0.019 0.027 0.003 0.006 0.006 0.006

Otu0003411 unclassified unclassified unclassified unclassified unclassified - - - - - 4.1 0.006 0.003 0.012 0.010 0.000 0.009 0.005 0.010 0.000 0.007 0.027 0.022 0.001 0.002 0.008 0.004

Otu0003472 unclassified unclassified unclassified unclassified unclassified - 3.0 - - 2.7 - 0.000 0.011 0.007 0.001 0.000 0.000 0.000 0.000 0.000 0.026 0.007 0.002 0.000 0.000 0.000 0.000

Otu0003733 unclassified unclassified unclassified unclassified unclassified - - - - 2.2 - 0.001 0.001 0.005 0.006 0.001 0.002 0.001 0.001 0.002 0.004 0.014 0.009 0.004 0.004 0.003 0.002

Otu0004101 unclassified unclassified unclassified unclassified unclassified - - 5.0 - - 6.2 0.000 0.000 0.000 0.029 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.095 0.000 0.000 0.000 0.000

Otu0004302 unclassified unclassified unclassified unclassified unclassified - - - - - 3.9 0.000 0.001 0.006 0.003 0.000 0.000 0.002 0.000 0.001 0.008 0.026 0.031 0.002 0.006 0.009 0.002

Otu0004476 unclassified unclassified unclassified unclassified unclassified - 4.0 3.8 - 3.9 4.2 0.000 0.002 0.016 0.013 0.000 0.000 0.000 0.000 0.001 0.006 0.024 0.030 0.000 0.001 0.001 0.001

Otu0004649 unclassified unclassified unclassified unclassified unclassified 5.1 4.0 - 4.8 5.1 - 0.000 0.034 0.016 0.002 0.000 0.000 0.000 0.000 0.001 0.049 0.061 0.003 0.000 0.000 0.000 0.000

Otu0004762 unclassified unclassified unclassified unclassified unclassified - - - - - 2.9 0.000 0.002 0.002 0.003 0.001 0.003 0.001 0.003 0.002 0.001 0.009 0.024 0.001 0.003 0.002 0.003

Otu0005003 unclassified unclassified unclassified unclassified unclassified - - - - - 2.7 0.003 0.004 0.005 0.004 0.004 0.005 0.003 0.006 0.000 0.004 0.001 0.007 0.002 0.002 0.001 0.000

Otu0005465 unclassified unclassified unclassified unclassified unclassified - 3.1 2.7 - 3.3 3.1 0.001 0.004 0.015 0.011 0.000 0.004 0.001 0.001 0.000 0.006 0.015 0.013 0.000 0.002 0.003 0.001

Otu0005777 unclassified unclassified unclassified unclassified unclassified - - - - - 3.1 0.000 0.001 0.003 0.004 0.000 0.000 0.001 0.002 0.000 0.002 0.011 0.013 0.001 0.003 0.005 0.002

Otu0005979 unclassified unclassified unclassified unclassified unclassified - - - - - 2.3 0.000 0.001 0.002 0.003 0.002 0.001 0.001 0.000 0.001 0.002 0.002 0.009 0.002 0.002 0.003 0.001

Otu0006223 unclassified unclassified unclassified unclassified unclassified - 3.5 3.8 - 2.6 3.2 0.001 0.005 0.017 0.021 0.000 0.002 0.002 0.000 0.000 0.002 0.007 0.011 0.000 0.000 0.000 0.000

Otu0006685 unclassified unclassified unclassified unclassified unclassified - 3.8 - - 5.2 - 0.000 0.000 0.014 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.046 0.001 0.000 0.000 0.000 0.000

Otu0006780 unclassified unclassified unclassified unclassified unclassified - - 4.7 - - - 0.000 0.000 0.000 0.024 0.000 0.000 0.001 0.000 0.000 0.000 0.000 0.018 0.000 0.000 0.000 0.000

Otu0006988 unclassified unclassified unclassified unclassified unclassified - 4.2 - - 3.9 - 0.000 0.001 0.019 0.002 0.000 0.000 0.000 0.000 0.000 0.006 0.018 0.005 0.000 0.000 0.000 0.000

Otu0007024 unclassified unclassified unclassified unclassified unclassified - - - - - 3.6 0.000 0.000 0.001 0.001 0.000 0.000 0.001 0.000 0.001 0.006 0.011 0.025 0.003 0.004 0.003 0.004

Otu0007045 unclassified unclassified unclassified unclassified unclassified - - 3.1 - 2.6 3.2 0.001 0.001 0.004 0.012 0.000 0.000 0.001 0.000 0.001 0.003 0.014 0.021 0.001 0.001 0.001 0.001

Otu0007162 unclassified unclassified unclassified unclassified unclassified - - 2.6 - - - 0.000 0.002 0.001 0.005 0.000 0.001 0.001 0.000 0.000 0.000 0.002 0.008 0.000 0.002 0.002 0.003

Otu0007202 unclassified unclassified unclassified unclassified unclassified - - - - - 2.1 0.001 0.000 0.003 0.002 0.001 0.000 0.003 0.003 0.000 0.003 0.002 0.006 0.001 0.002 0.001 0.000

Otu0007891 unclassified unclassified unclassified unclassified unclassified - - - - 3.1 3.9 0.000 0.001 0.002 0.004 0.000 0.000 0.000 0.000 0.000 0.001 0.013 0.025 0.001 0.002 0.002 0.002

Otu0008028 unclassified unclassified unclassified unclassified unclassified - - - - 2.6 4.7 0.001 0.000 0.001 0.008 0.000 0.000 0.000 0.001 0.000 0.002 0.007 0.033 0.000 0.000 0.000 0.000

Otu0008201 unclassified unclassified unclassified unclassified unclassified - - - - - 3.3 0.000 0.000 0.002 0.001 0.000 0.000 0.000 0.000 0.000 0.000 0.005 0.012 0.000 0.000 0.000 0.001

Otu0008653 unclassified unclassified unclassified unclassified unclassified - - 2.7 - - - 0.000 0.001 0.011 0.005 0.000 0.000 0.000 0.000 0.000 0.001 0.010 0.004 0.000 0.000 0.000 0.000

Otu0008874 unclassified unclassified unclassified unclassified unclassified - - 3.1 - - 4.7 0.000 0.000 0.001 0.007 0.000 0.000 0.000 0.000 0.000 0.000 0.005 0.032 0.000 0.000 0.000 0.000

Otu0008978 unclassified unclassified unclassified unclassified unclassified - - - - - 3.2 0.000 0.000 0.002 0.002 0.000 0.000 0.001 0.000 0.001 0.004 0.010 0.021 0.001 0.002 0.002 0.004

Otu0009272 unclassified unclassified unclassified unclassified unclassified - - - - - 2.1 0.000 0.001 0.003 0.003 0.001 0.000 0.001 0.001 0.000 0.003 0.006 0.006 0.000 0.001 0.000 0.001

Otu0009332 unclassified unclassified unclassified unclassified unclassified - - - - - 2.4 0.000 0.000 0.001 0.002 0.001 0.002 0.001 0.000 0.000 0.000 0.002 0.006 0.000 0.003 0.002 0.001

Otu0009536 unclassified unclassified unclassified unclassified unclassified - - 5.0 - - 4.3 0.000 0.000 0.003 0.030 0.000 0.000 0.000 0.000 0.000 0.000 0.002 0.025 0.000 0.000 0.000 0.000

Otu0009558 unclassified unclassified unclassified unclassified unclassified - - - - 3.0 3.4 0.000 0.000 0.003 0.005 0.000 0.000 0.000 0.000 0.000 0.001 0.013 0.017 0.000 0.001 0.000 0.000

Otu0009937 unclassified unclassified unclassified unclassified unclassified - - 3.3 - - 2.8 0.000 0.002 0.004 0.008 0.001 0.000 0.000 0.001 0.001 0.002 0.004 0.013 0.000 0.001 0.000 0.000

Otu0010349 unclassified unclassified unclassified unclassified unclassified - - 2.9 - 2.8 3.9 0.000 0.001 0.003 0.006 0.000 0.000 0.000 0.000 0.000 0.001 0.008 0.018 0.000 0.000 0.000 0.001

Otu0010420 unclassified unclassified unclassified unclassified unclassified - - - - - 3.8 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.003 0.003 0.023 0.001 0.002 0.002 0.003

Otu0010809 unclassified unclassified unclassified unclassified unclassified - - - - 3.1 - 0.000 0.001 0.002 0.002 0.000 0.000 0.000 0.000 0.000 0.004 0.010 0.006 0.000 0.002 0.001 0.001

Otu0010835 unclassified unclassified unclassified unclassified unclassified - - - - 2.4 - 0.001 0.002 0.003 0.002 0.000 0.000 0.003 0.000 0.000 0.004 0.008 0.001 0.000 0.003 0.000 0.000

Otu0010858 unclassified unclassified unclassified unclassified unclassified - - - - - 2.6 0.000 0.000 0.000 0.001 0.000 0.000 0.000 0.000 0.000 0.001 0.005 0.009 0.002 0.001 0.000 0.001

Otu0011837 unclassified unclassified unclassified unclassified unclassified - - 3.5 - 3.1 2.6 0.000 0.000 0.005 0.010 0.000 0.000 0.000 0.000 0.000 0.001 0.010 0.006 0.000 0.000 0.000 0.000

Otu0011908 unclassified unclassified unclassified unclassified unclassified - 4.2 - - - - 0.000 0.000 0.017 0.000 0.000 0.001 0.001 0.001 0.000 0.000 0.004 0.001 0.000 0.000 0.000 0.000

Otu0012712 unclassified unclassified unclassified unclassified unclassified - - - 2.5 - - 0.000 0.002 0.002 0.000 0.000 0.000 0.000 0.000 0.000 0.007 0.004 0.000 0.000 0.000 0.000 0.000

Otu0013955 unclassified unclassified unclassified unclassified unclassified - - 2.8 - - - 0.000 0.000 0.000 0.006 0.000 0.000 0.001 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000

Otu0014585 unclassified unclassified unclassified unclassified unclassified - - 3.5 - - 3.4 0.000 0.000 0.001 0.010 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.013 0.000 0.001 0.000 0.000

Otu0014697 unclassified unclassified unclassified unclassified unclassified - - 3.6 - - 3.5 0.000 0.000 0.002 0.010 0.000 0.000 0.000 0.000 0.000 0.000 0.003 0.014 0.000 0.000 0.000 0.000

Otu0016935 unclassified unclassified unclassified unclassified unclassified - - - - - 3.3 0.000 0.000 0.000 0.003 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.012 0.001 0.000 0.001 0.000

Otu0017216 unclassified unclassified unclassified unclassified unclassified - - - - - 2.4 0.000 0.000 0.002 0.001 0.000 0.000 0.000 0.000 0.000 0.000 0.001 0.006 0.000 0.000 0.003 0.001

Otu0017537 unclassified unclassified unclassified unclassified unclassified - - 2.7 - - - 0.000 0.000 0.000 0.005 0.000 0.000 0.000 0.000 0.000 0.001 0.001 0.007 0.000 0.000 0.000 0.000

Otu0006650 Verrucomicrobia Opitutae Opitutales Opitutaceae unclassified - - - - 2.3 - 0.000 0.002 0.002 0.000 0.001 0.001 0.000 0.000 0.000 0.005 0.007 0.004 0.000 0.002 0.000 0.000

Otu0001461 Verrucomicrobia Subdivision3 unclassified unclassified unclassified 2.9 - - - - - 0.001 0.014 0.003 0.001 0.004 0.002 0.004 0.005 0.027 0.006 0.007 0.016 0.026 0.015 0.023 0.010

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Otu0003341 Verrucomicrobia Subdivision5 unclassified unclassified unclassified - - 2.9 - 2.4 2.8 0.002 0.002 0.008 0.019 0.001 0.001 0.001 0.001 0.003 0.006 0.019 0.028 0.001 0.003 0.003 0.000

Otu0003551 Verrucomicrobia Subdivision5 unclassified unclassified unclassified - 2.5 5.2 - 2.6 4.5 0.000 0.000 0.006 0.045 0.000 0.002 0.000 0.000 0.000 0.002 0.009 0.038 0.000 0.001 0.000 0.001

Otu0008445 Verrucomicrobia Subdivision5 unclassified unclassified unclassified - - 2.4 - - 3.0 0.001 0.000 0.001 0.009 0.000 0.000 0.001 0.000 0.000 0.002 0.007 0.012 0.000 0.000 0.000 0.000

Otu0010778 Verrucomicrobia Subdivision5 unclassified unclassified unclassified - - 2.9 - - 3.0 0.001 0.001 0.002 0.011 0.000 0.000 0.000 0.000 0.000 0.000 0.001 0.010 0.000 0.000 0.000 0.000

Otu0018899 Verrucomicrobia Subdivision5 unclassified unclassified unclassified - - 3.1 - - - 0.000 0.001 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.009 0.003 0.001 0.000 0.000 0.000 0.000

Otu0002263 Verrucomicrobia Verrucomicrobiae Verrucomicrobiales Verrucomicrobiaceae Luteolibacter - 4.9 - - - - 0.001 0.001 0.056 0.015 0.034 0.020 0.001 0.030 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000

Representative sequence

OTU Name Sequence

Otu0002703 M01867_242_000000000_ATWYL_1_1101_7629_11480 .GAG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-C-T-CC--------------------------TG-TC-G-A--G-CGG----G--A-C--G---AA-TGCC-G---G-C-G--G-G--G-T--------------------A----------------------------AA--------------------------------C---A------------------G-CC---T-CGC-C-GG-T-T-G-A-C-G-----G-T---A-C-CG--------C-T-G-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGG------------------GCC-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-T---------------G--C-TG-C-T-C----------------------AA----G-T-C-G------------G-A-C--G--TG--A-AA-TC--C-C-TC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-G-G-G-A--A-C----T-G--C-G---T--C--------------C--GA-T-A---C---------------T--G-T--GC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--C-----C-CG------GA-G-A--------------G-G-G-T---AG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-CT-----------------------------G-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-G-G------------------------------------------------------------------------------G-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----C-AG--GG-G--AG-C-A-AA--CA

Otu0014689 M01867_242_000000000_ATWYL_1_1101_14322_22823 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-A-C-CC--------------------------TG-TC-G-T--C-AGG----G--A-C--G---AA--GAT-T---A-T-T--A-G--T-------------------T--T----------------------------AA--------------------------------T---A----G---------------AC---T-AGT-A-AT-T-T-G-A-C-G-----G-T---A-C-CT--------G-G-A-A---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GA-GC-GC-------G-TA-G-G-C-G---------------G--T-AT-T-T-T----------------------AA----G-T-G-A------------G-A-T--G--TG--C-AA-TC--C-C-CG-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-G-G-G-A--A-C----T-G--C-A---T--C--------------T--CA-T-A---C---------------T--G-G--GA--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-TG------GA-G-A--------------G-G-G-T---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-C-A-C---CTG-----G--AC-G-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-T----G-TG--GG-T--AG-C-A-AA--CA

Otu0005383 M01867_242_000000000_ATWYL_1_2102_15853_15111 .AGG--G-A-A--TT-TT--CG-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-C-G-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TT-G-T--T-AGG----G--A-A--G---AA-TGAT-C---A-G-G--A------------------------T------------------------------AGGAAAT----------------------------G--------A--------------------T-CTT-G-AA-G-T-G-A-C-G-----G-T---A-C-CT--------A-A-C-C---------A-G-----------A--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-TT-C-T-T----------------------AA----G-T-C-T------------T-A-T--G--TG--A-AA-TC--T-C-AG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-T-G-G-G--C------T-G--C-A---T--A--------------A--GA-A-A---C---------------T--A-T--TA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-AG------GA-G-A--------------G-G-G-C---AA-C--------------GG--A--ATT-----------C-C-A-T-GT--GT-A-G-CG-GT--A--------A-A-A---TG-C-GT-AG--AT-A-TA-----------------------------T-G---G-A--G-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--T-T-G-C---CTG-----G--CC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-AG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--TA

Otu0002701 M01867_242_000000000_ATWYL_1_1113_19097_5958 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-CCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-TC---C-----TA-TG-------G-A-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-A-T--A-CAC----C--A-A--G---AA-AAAC-T---C-C-C--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-GGG-A-GC-C-C-G-A-C-G-----G-T---A-G-TG--------T-A-T-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TC-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--C-TT-T-T-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--T-T-GC-C-G-----------------------------------------------------CT-T-AA-----------C-G-G-T-A-A-A--A-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-A--AG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AA------TT-G-A--------------G-G-T-A---AG-C--------------GG--A--ATG-----------T-G-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----AA-T--T--GC-GAA-G--G-C----A-----G--C-T-T-A---CTA-----A--AC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0007976 M01867_242_000000000_ATWYL_1_1107_22721_4761 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-CCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---C-----TA-TG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TT-TT-A-T--A-CAC----C--A-A--G---AA-AAAT-C---T-C-G--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-CGG-G-AC-T-C-G-A-C-G-----G-T---A-G-TG--------T-A-T-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TC-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--C-TT-T-T-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--C-T-GC-C-G-----------------------------------------------------CT-T-AA-----------C-G-G-T-A-G-A--A-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-A--GA--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AA------TT-G-A--------------G-G-T-A---AG-C--------------GG--A--ATG-----------T-G-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----AA-T--T--GC-GAA-G--G-C----A-----G--C-T-T-A---CTA-----A--AC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0000121 M01867_242_000000000_ATWYL_1_1101_19185_12310 .GAG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--T-G-CC---C-----TA-TG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-G-T--A-CGG----G--A-C--G---AA-ACTC-T---T-G-G--T---------------------------C----------------------------GT--------------------------------G-----------------------------A-CCA-G-GA-T-T-G-A-C-T-----G-T---A-C-CG--------T-G-C-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TC-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-AT-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-GC--C-T-GT-G-G-----------------------------------------------------CT-C-AA-----------C-C-A-T-A-G-A--A-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-AG------TT-G-A--------------A-G-T-G---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----AA-T--T--GC-GAA-G--G-C----A-----G--C-T-C-A---CTA-----A--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000312 M01867_242_000000000_ATWYL_1_1101_10372_2681 .GAG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--T-G-CC---C-----TA-TG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-G-T--A-CGG----G--A-C--G---AA-ACTC-C---T-G-G--T---------------------------C----------------------------GT--------------------------------G-----------------------------A-CCG-G-GA-T-T-G-A-C-T-----G-T---A-C-CG--------T-G-C-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-GC-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-GC--C-T-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TG--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-AG------TT-G-A--------------A-G-T-G---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----AA-T--T--GC-GAA-G--G-C----A-----G--C-T-C-A---CTA-----A--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000704 M01867_242_000000000_ATWYL_1_1102_10625_27763 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-CCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---C-----TA-TG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TT-TT-A-T--A-CAC----C--A-A--G---AA-AAAC-A---C-C-C--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-GGG-T-GC-C-C-G-A-C-G-----G-T---A-G-TG--------T-A-T-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TC-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--C-TT-T-T-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--T-T-GC-C-G-----------------------------------------------------CT-T-AA-----------C-G-G-T-A-A-A--A-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-A--TA--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AA------TT-G-A--------------G-G-T-A---AG-C--------------GG--A--ATG-----------T-G-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA-T--T--GC-GAA-G--G-C----A-----G--C-T-T-A---CTA-----A--AC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0001780 M01867_242_000000000_ATWYL_1_1101_15564_26029 .GAG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--T-G-CC---C-----TA-TG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-G-T--A-TGG----G--A-C--G---AA-ACTC-T---T-G-G--T---------------------------C----------------------------GT--------------------------------G-----------------------------A-CCG-A-GA-T-T-G-A-C-G-----G-T---A-C-CG--------T-A-C-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-AT-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-GC--C-T-GC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-T-A-G-A--A-A----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-AG------TT-G-A--------------A-G-T-G---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----AA-T--T--GC-GAA-G--G-C----A-----G--C-T-C-A---CTA-----A--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001871 M01867_242_000000000_ATWYL_1_1110_26335_11435 .GAG--G-A-A--TA-TT--GG-TC-------AA-T-G-G--GC---------------------------GA-G--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-CCC-G-C-G-T-----G-C-A--G--GA--------------------------A-G--A--C--G-G-CC---C-----TA-TG-------G-G-T--------T-G-TA-A---A--C-T-GC--------------------------TT-TT-T-T--A-TGG----G--A-A--G---AA-AAAG-C---T-C-T--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-AGG-G-CA-C-T-G-A-C-G-----G-T---A-C-CA--------T-A-T-G---------A-AT-------------AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCG-A-G------------C--G--T---T--AT-CCGG--AT------TT-A--T-T--AG-GT-------------------TT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--A-CT-G-T-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--T-C-AG-AG------------------------------------------------------CT-T-AA-----------C-T-C-T-G-A-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-A--CA--G-C-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AG------TT-G-A--------------A-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--GA---------A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA--T-T--GC-GAA-G--G-C----A-----G--CT--T-A---CTA-----A--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0002106 M01867_242_000000000_ATWYL_1_1107_21409_12231 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-A-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-A--G--GA--------------------------A-G--A--A--G-G-CC---C-----TA-AG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-G-T--A-CGG----G--G-G--T---AA-TTTA-C---G-G-A--T---------------------------C----------------------------GT--------------------------------G-----------------------------A-TCT-G-TA-T-T-G-A-A-A-----G-T---A-C-TG--------T-A-C-G---------A-A-----------T--AA-------------GC-G--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GAC------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TC-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--G-CC-T-T-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-GT--T-T-GC-C-G-----------------------------------------------------CT-T-AA-----------C-G-G-T-A-A-G--A-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-G--AG--G-C-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-AG------AT-G-A--------------G-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-GT--GT-A-G-TG-GT--G--------A-A-A---TA-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA-T--T--GC-GAA-G--G-C----A-----G--C-T-T-A---CTA-----A--AT-T-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0002159 M01867_242_000000000_ATWYL_1_1102_10421_20002 .AGG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-A-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---T-----TA-TG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TT-TT-G-T--A-CGG----G--A-A--G---AA-ACCC-T---C-G-A--A---------------------------C----------------------------GT--------------------------------G---T---------------------------TCG-G-GG-C-T-G-A-C-G-----G-T---A-C-CG--------T-A-A-G---------A-A-----------T--AA-------------GG-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G---GCG---------------G--A-TT-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--C-T-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-TG------TT-G-A--------------G-G-T-G---GG-C--------------GG--A--ATT-----------T-G-T-C-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TG-----------------------------A-C---A-A--A-G-A-AC-A-CC----AA-T--C--GC-GAA-G--G-C----A-----G--T-T-C-A---CCA-----A--AC-A-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0002784 M01867_242_000000000_ATWYL_1_1101_21102_15467 .GAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GA-G--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-TCC-G-C-G-T-----G-A-A--G--GA--------------------------A-G--A--A--G-G-CC---C-----TA-CG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-C-T--G-TAA----G--A-A--G---AA-AAGT-C---C-C-G--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-CGG-G-AT-G-T-G-A-C-G-----G-T---A-T-TA--------T-A-G-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-CA-G-G-T-G---------------G--T-TT-T-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-GT--C-T-GT-C-G-----------------------------------------------------CT-T-AA-----------C-G-A-T-A-G-G--A-T----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--AG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-GG------AT-G-A--------------G-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-AT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-T-CC----GA-T--T--GC-GAA-G--G-C----A-----G--C-T-T-A---CTA-----A--TC-C-A------------------------------------------------------------------------------C-C------A-C-T--GA--CA---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0003018 M04041_72_000000000_AUMPE_1_2108_13801_16694 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-CCC-G-C-G-T-----G-A-A--G--GA--------------------------A-G--A--C--G-G-CG---C-----TA-TG-------C-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-C-C--A-GAG----G--A-A--T---AA-AATT-T---T-G-T--A---------------------------CG----------------------------A--------------------------------G-----------------------------T-ACG-G-AA-C-T-G-A-A-G-----G-T---A-C-TC--------T-G-G-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-TT-A-A-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-AC--C-T-TC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-A-G-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-GG------TC-A-A--------------G-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA-T--T--GC-GAA-G--G-C----A-----G--C-T-T-A---CTG-----G--GC-C-A------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0004457 M01867_242_000000000_ATWYL_1_2104_7853_14669 .GAG--G-A-A--TA-TT--GG-TC-------AA-T-G-G--GC---------------------------GG-G--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-CCC-G-C-G-T-----G-C-A--G--GA--------------------------A-G--A--C--G-G-CC---C-----TA-TG-------G-G-T--------T-G-TA-A---A--C-T-GC--------------------------TT-TT-T-T--A-TGG----G--A-A--G---AA-AAAG-C---T-C-T--A---------------------------C----------------------------GT--------------------------------G-----------------------------T-AGG-G-CA-C-T-G-A-C-G-----G-T---A-C-CA--------T-A-T-G---------A-AT-------------AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCG-A-G------------C--G--T---T--AT-CCGG--AT------TT-A--T-T--AG-GT-------------------TT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--A-TT-G-T-T----------------------AA----G-T-C-A------------G-T-G--G--TG--A-AA-TC--T-C-AC-AG------------------------------------------------------CT-C-AA-----------C-T-G-T-G-A-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-G--TA--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AG------TT-G-A--------------A-G-T-A---GG-C--------------GG--A--ATG-----------T-G-T-A-AT--GT-A-G-CG-GT--GA---------A-A---TG-C-TT-AG--AT-A-TT-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA--T-T--GC-GAA-G--G-C----A-----G--CT--T-A---CTA-----A--GC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0006442 M01867_242_000000000_ATWYL_1_1106_14269_21883 .AAG--G-A-A--TA-TT--GG-T-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-A-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-CC---C-----TA-TG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-G-T--A-CGG----G--A-A--A---AA-ACCT-C---T-G-G--T---------------------------C----------------------------GT--------------------------------G---T-------------------------A-TCA-G-AG-C-T-G-A-T-G-----G-T---A-C-CG--------T-A-A-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TC-A--T-T--GG-GT-------------------TT--A-----AA-GG-GT-GC-------G-CA-G---GCG---------------G--A-AT-A-A-T----------------------AA----G-T-C-A------------G-C-G--G--TG--A-AA-GC--C-T-AC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-A-G-A--A-C----T-G--C-C---A--T--------------T--GA-T-A---C---------------T--G-T--TA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----T-TG------TT-G-A--------------G-G-T-T---GG-C--------------GG--A--ATG-----------T-G-T-C-AT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TT-AG--AT-A-TG-----------------------------A-C---A-C--A-G-A-AC-A-CC----GA-T--C--GC-GAA-G--G-C----A-----G--C-T-A-A---CCA-----A--GC-A-A------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CA

Otu0002933 M01867_242_000000000_ATWYL_1_1102_12145_15866 .GAG--G-A-A--TC-TT--CC-A-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-G-G-A---GC-A-----A--C-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TT-TT-A-T--C-TGT----G--A-T--G---A----------------------------------------------A----------------------------TA---------------------------------------------------------------------------T-G-A-C-A-----G-T---A-A-CA--------G-A-A-G---------A-A-----------T--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GGT--C-AT-------AC---GTAGG-G-T------------------GCA-A-G------------C--A--T---T--AT-C-CGG-AG------TG-A--C-T--GG-GC-------------------GT--A-----AA-GA-GTTGC-------G-TA-G-G-T-G---------------G--T-TT-G-T-T----------------------AA----G-T-G-G------------A-T-G--G--TG--A-AA-TT--T-C-GT-G-G-----------------------------------------------------CT-C-AA-----------C-C---GCG-A-A--A-C----T-A--T-T---A--T--------------C--CA-A-A---C---------------T--G-A--CA--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-A--A-----T-AG------CA-G-A--------------G-G-T-A---AC-T--------------GG--A--ATT-----------T-C-T-A-GT--GT-A-G-GG-GT--A--------A-A-A---TC-C-GT-AG--AT-A-TT-----------------------------A-G---A-A--G-G-A-AC-A-CC----AA-T--G--GC-GTA-G--G-C----A-----G--G-T-T-A---CTG-----G--GC-T-A------------------------------------------------------------------------------T-T------T-C-T--GA--CA---C----------------------------T-A--A-AG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CC

Otu0000209 M01867_242_000000000_ATWYL_1_1101_7807_9648 .AGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-C-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-GC--------------------------TT-TT-A-G--A-GGA----G--A-T--G---A----------------------------------------------G----------------------------AA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-T-CC--------T-C-T-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-GAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCT-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GT-GT-------G-CA-G-G-C-G---------------G--T-TT-G-G-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-A-A---C---------------T--A-C--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-A--A-----T-GG------GA-G-A--------------G-G-G-A---GA-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-A--G-C----G-----G--T-C-T-C---CTA-----G--AC-C-A------------------------------------------------------------------------------T-T------T-C-T--GA--CG---C----------------------------T-T--A-AA--C-A-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0001154 M01867_242_000000000_ATWYL_1_1107_22854_13120 .AGG--G-A-A--TA-TT--GG-T--------AA-T-C-T--GC---------------------------GA-A--A--G-C--G-G-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TT-T-G--G-GGG----G--A-T--G---A----------------------------------------------G----------------------------GT--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-C-CT--------C-C-A-G---------A-A-----------T--AA-------------GT-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GAG------------------ACT-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TT-G-G-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--G-T--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-A--A-----T-GG------AA-G-A--------------G-G-A-A---GG-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----A--C-C-T-T---CTG-----G--TC-C-A------------------------------------------------------------------------------T-T------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0001284 M01867_242_000000000_ATWYL_1_1101_11344_20412 .AGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TT-TT-C-T--G-GGA----G--A-T--G---A----------------------------------------------G----------------------------AA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-T-CC--------C-A-G-G---------A-A-----------T--AA-------------GT-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AG-GAG------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AT------TC-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--T-TC-G-G-T----------------------AA----G-T-T-G------------G-G-C--G--TG--A-AA-TC--T-C-CC-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-G-G-A-G--G-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--A-C--CG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-A--G-----C-GA------TA-G-A--------------G-G-A-A---AA-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-TG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----A--T-T-T-T---CTG-----G--AT-C-G------------------------------------------------------------------------------T-T------T-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0002560 M01867_242_000000000_ATWYL_1_1101_14577_4544 .AGG--G-A-A--TA-TT--GG-T--------AA-T-C-T--GC---------------------------GA-A--A--G-C--G-G-G-A-A-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TT-T-G--A-GGG----G--A-T--G---A----------------------------------------------C----------------------------GA--------------------------------A------------------------------------------G-G-A-C-A-----G-T---A-C-CC--------T-C-A-G---------A-A-----------T--AA-------------GC-C--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AA-------AC---GT-AG-GAG------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--T-TC-G-G-T----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--T-C-CT-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-G-G-A-G--A-G----G-T--C-G---T--T--------------C--AA-T-A---C---------------T--G-C--CG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-GA------GA-G-A--------------G-G-G-A---GG-T--------------GG--A--ATT-----------C-C-G-G-GT--GT-A-G-TG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----G--C-C-T-C---CTG-----G--CT-C-A------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-A--A-GA--C-G-CG-A--AA-G-C----T-AG--GG-T--AG-C-A-AA--CG

Otu0000433 M01867_242_000000000_ATWYL_1_1101_6314_23380 .GGG--G-A-A--TT-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-G--G--GA--------------------------T-G--A--A--G-G-CC---------TC-TG-------G-G-C--------T-G-T--A---AA-C-C-TC--------------------------TT-TT-C-T--C-AAG----G--A-A--G---A-------------------------------------------A--G----------------------------AC--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-TT--------G-A-G-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------T--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-GA-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GT-CC-------G-CA-G-G-C-G---------------G--C-CC-T-G-T----------------------AA----G-T-C-T------------G-T-C--G--TT--A-AA-GC--G-T-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-A-T-T--T-C----A-G--C-G---A--T--------------G--GA-A-A---C---------------T--A-C--AG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----T-GG------TA-G-G--------------G-G-C-A---GA-G--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----C--T-C-T-G---CTG-----G--GC-C-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-C--A-TG--G-A-CG-A--AA-G-C----C-AG--GG-G--AG-C-G-AA--AG

Otu0001438 M01867_242_000000000_ATWYL_1_1101_5536_8232 .GGG--G-A-A--TT-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--A--G-G-CC---------TC-TG-------G-G-C--------T-G-T--A---AA-C-C-TC--------------------------TT-TT-C-T--C-AAG----G--A-A--G---A-------------------------------------------A--G----------------------------AC--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-TT--------G-A-G-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------T--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-GA-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GT-CC-------G-CA-G-G-C-G---------------G--C-CT-T-G-A----------------------AA----G-T-C-T------------G-T-C--G--TC--A-AA-AC--G-T-GG-A-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-A-T-T--T-C----G-G--C-G---A--T--------------G--GA-A-A---C---------------T--G-T--AA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--G-----T-GG------TA-G-G--------------G-G-C-A---GA-G--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----C--T-C-T-G---CTG-----G--GC-C-A------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-C--A-TG--G-A-CG-A--AA-G-C----CAGG--GG----AG-C-G-AA--AG

Otu0000115 M01867_242_000000000_ATWYL_1_1101_23011_15108 .GGG--G-A-A--TT-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-A-----A--T-ACC-G-C-G-T-----G-A-G--G--GA--------------------------G-G--A--A--G-G-CT---------CT-TG-------G-G-T--------C-G-T--A---AA-C-C-TC--------------------------TT-TT-C-T--C-AAG----G-----------A-------------------------------------------A--G---------------------------ATA--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-TT--------G-A-G-G---------A-A-----------T--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TG-A--T-T--GG-GC-------------------GT--A-----AA-GA-GT-CC-------G-TA-G-G-T-G---------------G--T-AG-T-T-C----------------------AA----G-T-C-T------------G-T-G--G--TT--A-AA-GC--G-T-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-A-T-A--A-A----G-G--C-C---A--T--------------G--GA-A-A---C---------------T--G-A--AC--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----A-GG------TA-G-G--------------G-G-T-A---GA-G--------------GG--A--ATT-----------C-T-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-A--G-C----G-----C--T-C-T-A---CTG-----G--AC-C-T------------------------------------------------------------------------------T-A------A-C-T--GA--CA---C----------------------------T-G--A-GG--G-A-CG-A--AA-G-C----T-AG--GG-G--AG-C-G-AA--AG

Otu0003542 M01867_242_000000000_ATWYL_1_2106_24227_19439 .GGG--G-A-A--TT-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--GCC----T-G-A-C-G-G-A---GC-A-----A--G-ACC-G-C-G-T-----G-A-G--G--GA--------------------------G-G--A--A--G-G-CC---------CT-TG-------G-G-T--------T-G-T--A---AA-C-C-TC--------------------------TT-TT-A-T--T-AAG----G--A-A--G---A-------------------------------------------A--G----------------------------AA--------------------------------C------------------------------------------T-G-A-C-G-----G-T---A-C-TT--------A-A-T-G---------A-A-----------T--CA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GAT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TC-A--T-T--GG-GC-------------------GT--A-----AA-GA-GT-CC-------G-TA-G-G-T-G---------------G--T-CA-A-T-C----------------------AA----G-T-C-G------------G-C-C--G--TC--A-AA-GC--A-T-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-A-T-A--A-A----G-G--C-G---G--T--------------G--GA-A-A---C---------------T--G-G--TA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----T-AG------GA-G-G--------------G-G-T-A---GA-G--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC----GG-T--G--GC-GAA-A--G-C----G-----C--T-C-T-A---CTG-----G--AC-T-A------------------------------------------------------------------------------G-A------A-C-T--GA--CA---C----------------------------T-G--A-GG--G-A-CG-A--AA-G-C----T-AG--GG-G--AG-C-G-AA--AG

Otu0002196 M01867_242_000000000_ATWYL_1_1102_17613_15661 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-A-C-TC--------------------------TG-TC-C-T--A-GGG----G--A-A--GA--A-------------------------------------------A--A----------------------------AA--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-CC--------T-G-G-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-AAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-TT-A-A-C----------------------AA----G-C-C-A------------G-A-G--G--TG--A-AA-GG--C-A-TT-A-G-----------------------------------------------------CT-C-AA-----------C-T-A-A-T-G-T--A------A-G--C-C---T--T--------------T--GG-A-A---C---------------T--G-T--TT--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-AG------GA-G-A--------------G-G-T-A---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----A--C-T-T-A---CTG-----G--AC-T-G------------------------------------------------------------------------------A-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000341 M01867_242_000000000_ATWYL_1_2114_21475_18352 .GGG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--G--GA--------------------------C-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TG-A-T--C-AGG----G--A-C--G-----------------------------------------------A--A----------------------------TA--------------------------------A---A--------------------------------------T-G-A-C-G-----G-T---A-C-CT--------G-A-A-G---------A-A-----------C--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GT-------G-TA-G-G-C-G---------------G--C-TC-T-G-C----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TT--C-C-CG-G-G-----------------------------------------------------CT-T-AA-----------C-C-C-G-G-G-C--G-C----T-G--C-A---T--C--------------C--GA-A-A---C---------------T--G-C--AG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-TG------GA-G-A--------------G-G-A-T---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--A--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-T---CTG-----G--AC-A-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000349 M01867_242_000000000_ATWYL_1_1105_27639_21195 .GGG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--G--GA--------------------------C-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TG-A-T--C-AGG----G--A-C--G-----------------------------------------------A--A----------------------------TA--------------------------------A---A--------------------------------------T-G-A-C-G-----G-T---A-C-CT--------G-A-A-G---------A-A-----------C--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GT-------G-TA-G-G-C-G---------------G--C-TC-T-G-C----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-TT--C-C-CG-G-G-----------------------------------------------------CT-T-AA-----------C-C-C-G-G-G-C--G-C----T-G--C-A---T--C--------------C--AA-A-A---C---------------T--G-C--GG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-TG------GA-G-A--------------G-G-A-T---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--A--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-T---CTG-----G--AC-A-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002800 M01867_242_000000000_ATWYL_1_1108_5083_17806 .GGG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-C-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--G--GA--------------------------C-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TG-A-T--C-AGG----G--A-C--G-----------------------------------------------A--A----------------------------TA--------------------------------A---A--------------------------------------T-G-A-C-G-----G-T---A-C-CT--------G-A-A-G---------A-A-----------C--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GT-------G-TA-G-G-C-G---------------G--C-TC-T-G-C----------------------AA----G-T-T-G------------G-G-T--G--TG--A-AA-TT--C-C-CG-G-G-----------------------------------------------------CT-T-AA-----------C-C-C-G-G-G-C--G-C----T-G--C-A---T--C--------------C--AA-A-A---C---------------T--G-C--GG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-TG------GA-G-A--------------G-G-A-T---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--A--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-T---CTG-----G--AC-A-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0009600 M01867_242_000000000_ATWYL_1_1113_18548_5311 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-CT---------CT-CG-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TG-A-T--T-AGG----G--A-C--G---A----------------------------------------------A----------------------------TA--------------------------------A---A--------------------------------------T-G-A-C-G-----G-T---A-C-CT--------A-A-A-A---------A-A-----------C--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GC-GT-------G-TA-G-G-C-G---------------G--G-GA-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TT--C-C-AC-T-G-----------------------------------------------------CT-C-AA-----------C-G-G-T-G-G-C--G-C----T-G--C-A---T--C--------------T--GA-A-A---C---------------T--A-T--AT--C-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-TG------GA-G-A--------------G-G-A-A---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-A-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0011033 M01867_242_000000000_ATWYL_1_2105_7137_13983 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--A--G-G-CC---------TT-AG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TT-T-T--A-AGG----G--A-C--G---AA-TGTT-A---C-G-G--G-A--A-G-----------------T--A----------------------------AT--------------------------------A----------------------T-GT---C-CTG-T-AA-A-T-G-A-C-G-----G-T---A-C-CT--------T-A-A-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---GT-AG-GGG------------------GCG-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GA-GC-AC-------G-TA-G-G-C-G---------------G--G-AA-C-T-T----------------------AA----G-T-C-A------------G-G-T--G--TG--A-AA-GA--C-C-GA-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-C-G-G-G--G-T----G-G--C-A---C--T--------------T--GA-A-A---C---------------T--G-G--GT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--G-----C-AG------GA-G-A--------------G-G-A-A---AG-T--------------AG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AA-A-TC-----------------------------A-G---G-A--G-G-A-AT-A-CC----AG-T--G--GC-GAA-G--G-C----G-----A--C-T-T-T---CTG-----G--AC-T-G------------------------------------------------------------------------------A-C------C-C-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-T--AG-C-A-AA--CA

Otu0000389 M01867_242_000000000_ATWYL_1_1101_16099_2179 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-C-T--G-TGG----G--A-C--GA--A-------------------------------------------AC-A----------------------------AA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CA--------C-A-G-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-T-G---------------G--T-TC-C-T-C----------------------AA----G-T-C-A------------G-A-A--G--TG--A-AA-GG--C-A-GT-G-G-----------------------------------------------------CT-T-AA-----------C-C-A-C-T-G-T--A------A-G--C-T---T--T--------------T--GA-A-A---C---------------T--G-A--GG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-AG------GA-G-A--------------G-G-T-A---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----A--C-T-T-A---CTG-----G--AC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CA---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0001632 M01867_242_000000000_ATWYL_1_1101_9597_6989 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--T--GA--------------------------A-G--A--A--G-G-TC---------TT-CG-------G-A-T--------C-G-T--A---AA-G-T-TC--------------------------TT-TT-C-T--T-AGG----G--A-A--G---A----------------------------------------------A---------------------------ACC--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------A-A-G-G---------A-A-----------T--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-CT-G-A-C----------------------AA----G-T-C-A------------G-A-A--G--TG--A-AA-GG--C-A-GT-G-G-----------------------------------------------------CT-C-AA-----------C-C-A-T-T-G-T--A------A-G--C-T---T--T--------------T--GA-A-A---C---------------T--G-T--TA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--G-----C-AG------GA-G-G--------------G-G-C-A---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----A--C-T-T-G---CTG-----G--AC-T-G------------------------------------------------------------------------------T-A------A-T-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002104 M01867_242_000000000_ATWYL_1_1102_11968_17847 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------A-G--A--A--G-G-CT---------TT-CG-------G-G-T--------C-G-T--A---AA-G-T-TC--------------------------TT-TT-C-T--T-AGG----G--A-A--G---A----------------------------------------------A---------------------------ACC--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------A-A-G-G---------A-A-----------T--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-CT-G-A-C----------------------AA----G-T-C-A------------G-A-A--G--TG--A-AA-GG--C-A-GT-G-G-----------------------------------------------------CT-C-AA-----------C-C-A-T-T-G-T--A------A-G--C-T---T--T--------------T--GA-A-A---C---------------T--G-T--CG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--G-----C-AG------GA-G-G--------------G-G-C-A---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----A--C-T-T-G---CTG-----G--AC-T-G------------------------------------------------------------------------------T-A------A-T-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0003707 M01867_242_000000000_ATWYL_1_1111_7347_21776 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CT---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-C-T--A-GGG----G--A-A--G---A-------------------------------------------A--TA---------------------------AA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CC--------T-G-G-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-T-G---------------G--T-TC-G-A-C----------------------AA----G-T-C-A------------G-A-A--G--TG--A-AA-GG--C-T-AC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-T-G-G-T--A------A-G--C-T---T--T--------------T--GA-A-A---C---------------T--G-T--TG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-AG------GA-G-A--------------G-G-T-A---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----A--C-T-T-A---CTG-----G--AC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CA---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0006234 M01867_242_000000000_ATWYL_1_1103_28459_15243 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-C-T--A-TGG----G--A-A--GA--A-------------------------------------------A--A----------------------------AA--------------------------------A------------------------------------------T-G-A-C-G-----G-T---A-C-CA--------T-A-G-G---------A-G-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-T-G---------------G--T-TC-C-T-C----------------------AA----G-T-C-A------------G-A-A--G--TG--A-AA-GG--C-A-AT-G-G-----------------------------------------------------CT-T-AA-----------C-C-A-T-T-G-T--A------A-G--C-T---T--T--------------T--GA-A-A---C---------------T--G-A--GG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-AG------GA-G-A--------------G-G-T-A---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----A--C-T-T-A---CTG-----G--AC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CA---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----GTGG--GG----AG-C-G-AA--CA

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Chapter 3

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Otu0001463 M01867_242_000000000_ATWYL_1_1101_18769_7620 .GGG--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------CT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-T-T--C-AGG----G--A-C--G---AA-TGTG-C---A-G-A--T-T--G-------------------T--G----------------------------AA--------------------------------T---A----A---------------TG---G-TTT-G-TA-A-T-G-A-C-G-----G-T---A-C-CT--------G-A-G-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GC-------G-CA-G-G-C-G---------------G--G-AT-G-A-T----------------------AA----G-T-C-T------------G-T-C--T--TA--A-AA-GT--T-C-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-C-G-T-G--A------T-G--G-G---A--T--------------G--GA-A-A---C---------------T--A-T--CA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-CG------GA-G-A--------------G-G-A-A---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----A--C-T-T-T---CTG-----G--AC-G-A------------------------------------------------------------------------------T-G------T-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----C-AG--GG-T--AG-T-G-AA--CG

Otu0004716 M01867_242_000000000_ATWYL_1_1101_26680_11274 .GGG--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------CT-TG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-A-T--C-GGG----G--A-C--G---AA-CGGC-T---T-T-A--T-C--A-------------------G--GA---------------------------A---------------------------------A---T--------------------GG---G-TAG-A-GA-T-T-G-A-C-G-----G-T---A-C-CC--------G-A-G-A---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCG-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GC-------G-CA-G-G-T-G---------------G--G-AT-C-T-T----------------------AA----G-T-C-T------------G-T-T--C--TG--A-AA-GT--T-C-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-G-T-G--A------T-G--G-G---A--T--------------G--GA-A-A---C---------------T--G-G--GA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-CG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATT-----------C-C-C-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GCG-AA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-G-A------------------------------------------------------------------------------T-G------T-C-T--GA--CA---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----C-AG--GG-G--AG-C-G-AA--CG

Otu0006138 M01867_242_000000000_ATWYL_1_1103_6884_13863 .GGG--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------CT-TG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-A-T--T-GGG----G--A-C--G---AA-CGAC-C---G-C-T--T-C--A-------------------G--GA---------------------------A---------------------------------A---T--------------------GG---G-AGC-G-GA-G-T-G-A-C-G-----G-T---A-C-CC--------A-A-G-A---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GC-------G-CA-G-G-T-G---------------G--G-AT-C-T-T----------------------AA----G-T-C-T------------G-T-T--C--TG--A-AA-GT--T-C-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-G-T-G--A------T-G--G-G---A--T--------------G--GA-A-A---C---------------T--G-G--GA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-CG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATT-----------C-C-C-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GCG-AA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-G-A------------------------------------------------------------------------------T-G------T-C-T--GA--CA---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----T-AG--GG-G--AG-C-G-AA--CG

Otu0006141 M01867_242_000000000_ATWYL_1_1101_24389_15438 .GGG--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-TT---------TT-CG-------G-A-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-T-T--C-AGG----G--A-C--G---AA-TGTG-C---A-G-G--A-T--G-------------------T--A----------------------------AA--------------------------------T---A----A---------------TG---T-CTT-G-TA-A-T-G-A-C-G-----G-T---A-C-CT--------G-A-G-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GC-------G-CA-G-G-C-G---------------G--G-AT-G-G-T----------------------AA----G-T-C-T------------G-T-C--T--TA--A-AA-GT--T-C-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-C-G-T-G--A------T-G--G-G---A--T--------------G--GA-A-A---C---------------T--A-T--CA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----T-CG------GA-G-A--------------G-G-A-A---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----A--C-T-T-T---CTG-----G--AC-G-A------------------------------------------------------------------------------T-G------T-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----C-AG--GG-T--AG-T-G-AA--CG

Otu0011769 M01867_242_000000000_ATWYL_1_2102_10723_8517 .GGG--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------CT-TG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-T-T--T-GGG----G--A-C--G---AA-CGAT-C---G-C-G--T-C--A-------------------G--GA---------------------------A---------------------------------A---T--------------------GG---G-CGT-G-AA-G-T-G-A-C-G-----G-T---A-C-CC--------A-A-G-A---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GC-------G-CA-G-G-T-G---------------G--G-AT-C-T-T----------------------AA----G-T-C-C------------A-T-T--T--TG--A-AA-GT--T-C-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-G-T-G--A------T-G--G-G---A--T--------------G--GA-A-A---C---------------T--G-G--GA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-CG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATT-----------C-C-C-C-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GCG-AA-G--G-C----G-----G--C-T-T-T---CTG-----G--AC-G-G------------------------------------------------------------------------------T-G------T-C-T--GA--CA---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----C-AG--GG-G--AG-C-G-AA--CG

Otu0000139 M01867_242_000000000_ATWYL_1_1107_26301_7844 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--ACC--------------------------AA-A--A--G-T--C-T-G-A-T-C-C-A---GC-A-----A--T-TCT-G-T-G-T-----G-C-T--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-A-GC--------------------------TT-TA-A-G--T-AGG----G--A-A--G---AA---------------------------------------------G----------------------------AA--------------------------------A----------------------------------------G-T-G-A-C-G-----G-T---A-C-CT--------A-C-A-G---------A-A-----------T--AA-------------GC-G--AC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GTC------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--T-T--GG-GC-------------------AT--A-----AA-GG-GC-GT-------C-TA-G-G-C-G---------------G--T-TT-A-G-A----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-AT--G-C-GG-A-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-G-T-A--T------T-G--C-G---T--T--------------G--GA-A-A---C---------------T--A-C--TA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-TG------GA-G-A--------------G-G-T-A---AG-C--------------GG--A--ACT-----------A-C-A-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------T-G---T-A--G-G-A-AT-G-CC---AAT----T--GG-GAA-G--C-C----A-----G--C-T-T-A---CTG-----G--AC-A-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-AG--C-G-CG-A--AA-G-C----T-AG--GG-G--AG-C-A-AA--CA

Otu0000344 M01867_242_000000000_ATWYL_1_1101_17759_5237 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--ACC--------------------------AA-A--A--G-T--C-T-G-A-T-C-C-A---GC-A-----A--T-TCT-G-T-G-T-----G-C-T--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-A-GC--------------------------TT-TA-A-G--T-AGG----G--A-A--G---AA---------------------------------------------G----------------------------AA--------------------------------A----------------------------------------G-T-G-A-C-G-----G-T---A-C-CT--------A-C-A-G---------A-A-----------T--AA-------------GC-G--AC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GTC------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--T-T--GG-GC-------------------AT--A-----AA-GG-GC-GT-------C-TA-G-G-C-G---------------G--T-TT-A-G-A----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-AT--G-C-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-C-G-T-A--T------T-G--C-G---T--T--------------G--GA-A-A---C---------------T--A-C--TA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-CG------GA-G-A--------------G-G-T-A---AG-C--------------GG--A--ACT-----------A-C-A-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------T-G---T-A--G-G-A-AT-G-CC---AAT----T--GG-GAA-G--C-C----A-----G--C-T-T-A---CTG-----G--AC-G-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-AG--C-G-CG-A--AA-G-C----T-AG--GG-G--AG-C-A-AA--CA

Otu0009344 M01867_242_000000000_ATWYL_1_2113_11098_20017 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--ACC--------------------------AA-A--A--G-T--C-T-G-A-T-C-C-A---GC-A-----A--T-TCT-G-T-G-T-----G-C-T--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-A-GC--------------------------TT-TA-A-G--T-AGG----G--A-A--G---AA---------------------------------------------G----------------------------AA--------------------------------A----------------------------------------G-T-G-A-C-G-----G-T---A-C-CT--------A-C-A-G---------A-A-----------T--AA-------------GC-G--AC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GTC------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--T-T--GG-GC-------------------AT--A-----AA-GG-GC-GT-------C-TA-G-G-C-G---------------G--T-TT-A-G-A----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-AT--G-C----A-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-G-T-A--T------T-G--C-G---T--T--------------G--GA-A-A---C---------------T--A-C--TA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-TG------GA-G-A--------------G-G-T-A---AG-C--------------GG--A--ACT-----------A-C-A-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------T-G---T-A--G-G-A-AT-G-CC---AAT----T--GG-GAA-T--C-C----A-----G--C-T-T-A---CTG-----G--AC-A-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-AG--C-G-CG-A--AA-G-C----T-AG--GG-G--AG-C-A-AA--CA

Otu0003625 M01867_242_000000000_ATWYL_1_2106_27201_15992 .CGA--G-A-A--TC-TT--GG-G-C------AA-T-G-C--GC---------------------------GA-A--A--G-C--G-T-G-A-C-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TG-TC-A-C--C-CGG----G--A-C--G---AA--------------------------------------------------------------------------GA--------------------------------AT-----------------------------------------T-G-A-C-G-----G-T---A-C-CG--------G-G-A-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-CT-------G-TA-G-G-G-G---------------G--T-TT-G-C-T----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-TC--C-C-GT-G-G-----------------------------------------------------CT-C-AA-----------C-C-A-C-G-G-A--A-C----T-G--C-A---T--T--------------G--GA-G-A---C---------------T--G-G--CG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-TG------GA-G-A--------------G-G-T-A---AG-C--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC---GAT----A--GC-GAA-G--G-C----A-----G--C-T-T-A---CTG-----G--AC-A-G------------------------------------------------------------------------------C-G------A-C-T--GA--CC---C----------------------------T-G--A-AA--G-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002416 M01867_242_000000000_ATWYL_1_1105_12649_4401 .CGA--G-A-A--TC-TT--CG-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-C-G-A---GC-G-----A--T-GCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-C-C-TC--------------------------TG-TC-A-C--C-CGG----G--A-C--G---AA------------------------------------------G--A----------------------------AT--------------------------------G------------------------------------------T-G-A-C-G-----G-T---A-C-CG--------G-G-A-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-CC-------G-CA-G-G-G-G---------------G--T-TT-G-C-C----------------------AA----G-T-C-C------------G-A-T--G--TG--A-AA-TA--C-C-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-G-G-A--A-C----G-G--C-A---T--T--------------G--GA-T-A---C---------------T--G-G--CG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-AT------GA-T-G--------------G-G-T-G---AG-C--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC---GAT----G--GC-GAA-G--G-C----G-----G--C-T-C-A---CTG-----G--CG-T-G------------------------------------------------------------------------------C-A------A-C-T--GA--CC---C----------------------------T-C--A-GG--G-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004551 M01867_242_000000000_ATWYL_1_1105_25198_16124 .CGA--G-A-A--TA-TT--CG-A-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-C-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-T-GC---------TT-CG-------G-T-A--------T-G-T--A---AA-C-T-GC--------------------------TT-TT-G-T--G-AAC----C--A-G--A---AA---------------------------------------------GT---------------------------TT------------------------------------A------------------------------------T-T-G-A-T-C-----G-----G-T-TC----------A-A-G---------A-A-----------T--AA-------------GA-G--GT-T---G-C-TAA-----------------------A--C-T-C--G-T---------G-C-CA--G-C---A--G-C--AG---C-----------------GG--TA-AT-------AC---GA-GT-GCC------------------TCG-A-G------------C--G--T---T--AT-C-CGG-AA------TC-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GT-------G-CA-G-G-C-G---------------G--T-CT-G-A-T----------------------TA----G-T-C-T------------T-C-T--G--TT--A-AA-TT--T-C-TC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-G-G-A-A--C-C----T-G--C-A---G--G--------------G--GA-A-A---C---------------G--G-T--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--A-----T-GC------GA-G-A--------------G-G-T-T---AA-C--------------GG--A--ACT-----------C-A-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-TG--AT-A-TT-----------------------------A-T---G-G--G-G-A-AC-A-CC----AA-A--T--GC-GAA-G--G-C----A-----G--T-T-A-A---CTG-----G--AG-C-A------------------------------------------------------------------------------C-T------C-C-T--GA--CG---C----------------------------T-G--A-AA--C-A-CG-A--AA-G-C----G-TG--GG-T--AG-C-G-AA--TG

Otu0002854 M01867_242_000000000_ATWYL_1_1101_6630_12930 .CGA--G-A-A--TC-TT--CG-G-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-C-C-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-CC--------------------------TG-TC-G-A--G-GGG----G--A-A--C---AA--GGG-G---T------G---------------------------G--------------------------TGCAG-------------------------------A-----------------------------GCC-A-T-CC-T-T-G-A-G-T-----G-A---T-C-CC--------T-G---G---------A-G-----------G--AA-------------GC-A--CG-G---G-C-TAA-----------------------G--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---GA-AC-CGT------------------GCG-A-A------------C--G--T---T--AT-T-CGG-AT------TC-A--C-T--GG-GC-------------------TT--A-----AA-GC-GC-GT-------G-CA-G-G-C-G---------------G--C-CC-G-G-C----------------------AC----G-T-C-G------------GTC-G--C--TG--A-AA-GC--C-C-CC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-G-G-G-A--A-T----G-G--G-C---G--C--------------C--GA-T-A---C---------------G--A-C--CG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--G-----C-GG------TA-G-G--------------G-G-G-A---GC-T--------------GG--A--ACT-----------T-C-C-G-GT--GG-A-G-CG-GT--G--------A-A-A---TG-C-GT-TG--AG-A-TC-----------------------------G-G---A-A--G-G-A-AC-G-CC----CG-T--G--GC-GAA-A--G-C----G-----A--G-C-T-C---CTG-----G--AC-C-G------------------------------------------------------------------------------C-C------C-C-T--GA--CG---C----------------------------T-C--A-GA--C-G-CG-A--AA-G-C----C-AG--GG-G--AG-C-G-AA--CG

Otu0013998 M01867_242_000000000_ATWYL_1_2113_18932_14664 .CGA--G-A-A--TC-TT--CG-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-C-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-C-G--G--GA--------------------------T-G--A--A--G-G-CC---------CT-CG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TG-TC-A-G--A-GGA----A--T-G--G---AA-ATAT-T---G-G-G--G-G--G----------------------T----------------------------TC--------------------------------T------------------------CC---C-CTC-A-GT-T-T-G-A-C-T-----G-A---T-C-CT--------C-A---G---------A-G-----------G--AA-------------GT-A--CG-G---G-C-TAA-----------------------G--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---GA-AC-CGT------------------ACG-A-A------------C--G--T---T--AC-T-CGG-AA------TC-A--C-T--GG-GC-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-T---------------G--T-CC-G-G-T----------------------AA----G-T-T-G------------G-G-T--G--TG--A-AA-GC--C-C-TC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-A-G-G-A--A-T----A-G--C-G---C--C--------------C--AA-A-A---C---------------T--GTC--GG----A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--G-----A-GA------CA-G-A--------------G-G-T-G---AG-C--------------GG--A--ACT-----------T-A-G-G-GT--GG-A-G-CG-GT--G--------A-A-A---TG-C-GT-TG--AT-A-TC-----------------------------C-T---A-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-A--G-C----G-----G--C-T-C-A---CTG-----G--GT-C-T------------------------------------------------------------------------------C-T------T-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----T-AG--GG-G--AG-C-G-AA--CG

Otu0000085 M01867_242_000000000_ATWYL_1_1101_9712_22855 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-AG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-C--A-CGT----G--A-C--G---A----------------------------------------------T----------------------------GA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-A-CG--------T-G-A-G---------A-A-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GGG------------------GCT-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TC-T-T-T----------------------TA----G-T-C-A------------G-G-C--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-G---C--T--------------T--GA-T-A---C---------------T--G-G--AG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-CG------GA-G-A--------------G-G-A-T---GG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-A-T---CTG-----G--AC-G-G------------------------------------------------------------------------------A-C------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001917 M01867_242_000000000_ATWYL_1_1102_25811_21629 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GG---------------------------GC-A--A--C-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-AG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-C--A-CGC----G--A-C--G---A----------------------------------------------T----------------------------GA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-G-CG--------T-G-A-G---------A-A-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GGG------------------GCT-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TC-T-T-T----------------------TA----G-T-C-A------------G-G-C--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----C-G--C-G---C--T--------------T--GA-T-A---C---------------T--G-G--AG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-CG------GA-G-A--------------G-G-A-T---GG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-A-T---CTG-----G--AC-G-G------------------------------------------------------------------------------A-C------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0003281 M01867_242_000000000_ATWYL_1_1103_25577_8636 .CGA--G-G-A--TC-TT--GG-A-C------AA-T-G-T--GC---------------------------GA-A--A--G-C--A-T-G-A-T-C-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--C--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TG-TC-G-T--G-AGG----G--A-A--C---AA-TGGC-G---C-G-G--G-C--G-------------------A--T----------------------------AA--------------------------------C---A--------------------CC---GTTCG-C-GT-G-T-G-A-T-A-----G-T---A-C-CT--------C-A-G-G---------A-G-----------G--AA-------------GA-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTG------------------TCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-TT-C-G-T----------------------GT----G-T-C-G------------G-A-C--G--TG--A-AA-TC--C-T-GC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-C-A-G-A--A-C----G-G--C-G---T--C--------------C--GA-A-A---C---------------T--A-C--GG--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-GG------GA-G-A--------------G-G-A-A---GT-C--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--A-C-T-T---CTG-----G--AC-C-G------------------------------------------------------------------------------A-C------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AG-G-C----C-GG--GG-G--AG-C-A-AA--CA

Otu0006228 M01867_242_000000000_ATWYL_1_1101_11816_14561 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GG---------------------------GC-A--A--C-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-AG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-C--A-CGC----G--A-C--G---A----------------------------------------------T----------------------------GA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-G-CG--------T-G-A-G---------A-A-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GGG------------------GCT-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-CC-T-T-T----------------------AA----G-T-C-A------------G-G-C--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-G---C--T--------------T--GA-T-A---C---------------T--G-G--GG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-CG------GA-G-A--------------G-G-A-T---GG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-A-T---CTG-----G--AC-G-G------------------------------------------------------------------------------A-C------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0016688 M04041_72_000000000_AUMPE_1_1114_10631_8611 .GGG--G-A-A--TT-TT--CC-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-AG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-C--A-CGT----G--A-C--G---A----------------------------------------------T----------------------------GA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-A-CG--------T-G-A-G---------A-A-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GGG------------------GCT-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TC-T-T-T----------------------TA----G-T-C-A------------G-G-C--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-G---C--T--------------T--GA-T-A---C---------------T--G-G--AG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-CG------GA-G-A--------------G-G-A-T---GG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-A-T---CTG-----G--AC-G-G------------------------------------------------------------------------------A-C------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0027482 M04041_72_000000000_AUMPE_1_1113_2902_17239 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-AG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-C--A-CGT----G--A-C--G---A----------------------------------------------T----------------------------GA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-A-CG--------T-G-A-G---------A-A-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GGG------------------GCT-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TC-T-T-T----------------------TA----G-T-C-A------------G-G-C--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-G---C--T--------------T--GA-T-A---C---------------T--G-G--AG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-CG------GA-G-A--------------G-G-A-T---GG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-A-T---CTG-----G--AC-G-G------------------------------------------------------------------------------A-C------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----T-GG--GG-G--AG-C-A-AA--CA

Otu0032692 M04041_72_000000000_AUMPE_1_2102_18985_17686 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-AG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-C--A-CGT----G--A-C--G---A----------------------------------------------T----------------------------GA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-A-CG--------T-G-A-G---------A-A-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GGG------------------GCT-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TC-T-T-T----------------------TA----G-T-C-A------------G-G-C--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-G---C--T--------------T--GA-T-A---C---------------T--G-G--AG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-CG------GA-G-A--------------G-G-A-T---GG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-A-T---CTG-----G--AC-G-G------------------------------------------------------------------------------A-C------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001918 M01867_242_000000000_ATWYL_1_1101_15682_6246 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GG---------------------------GC-A--A--C-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-A-G--A-CGT----G--A-C--G---A----------------------------------------------T----------------------------GA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-G-CG--------T-C-A-G---------A-A-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GGG------------------GCT-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GC-------G-TA-G-G-C-G---------------G--C-GA-C-A-T----------------------CA----G-T-C-A------------G-A-G--G--TG--A-AA-TC--C-C-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-C-G-G-C--A-T----T-G--C-C---T--T--------------T--GA-T-A---C---------------T--G-T--GT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-GA------GA-G-A--------------G-G-G-T---GG-C--------------GG--A--ATA-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-T--A-G-A-AC-A-CC---AGT----G--GC-GAA-G--G-C----G-----G--C-C-A-C---CTG-----G--CT-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000755 M01867_242_000000000_ATWYL_1_1101_8289_9601 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-AG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-C--A-CGC----G--A-C--G---A----------------------------------------------T----------------------------GA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-G-CG--------T-G-A-G---------A-A-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GGG------------------GCT-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-CC-T-T-T----------------------TA----G-T-C-A------------G-G-C--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----C-G--C-G---C--T--------------T--GA-T-A---C---------------T--G-G--AG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-T--T-----C-CG------GA-G-A--------------G-G-A-T---GG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-A-T---CTG-----G--AC-G-G------------------------------------------------------------------------------A-C------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000364 M01867_242_000000000_ATWYL_1_1105_23214_12975 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-A--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-G-G--T-GGG----G--A-C--G---A----------------------------------------------T----------------------------GA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CC--------A-C-A-G---------A-A-----------T--AA-------------GC-T--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GGA------------------GCT-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TT-G-A-C----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--C-C-AG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-T-G-G-A--A-T----T-G--C-A---T--T--------------C--AA-G-A---C---------------T--G-T--CT--T-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--T-----C-GG------TA-G-A--------------G-G-T-G---AG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-C-A---CTG-----G--AC-C-G------------------------------------------------------------------------------A-C------A-T-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002590 M01867_242_000000000_ATWYL_1_1109_8228_15712 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-G-G--C-GGG----G--A-C--G---A----------------------------------------------T----------------------------GA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CC--------G-C-A-G---------A-A-----------T--AA-------------GC-T--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GGA------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GA-GT-GC-------G-TA-G-G-T-G---------------G--T-TT-G-G-C----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--C-C-TG-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-A-G-G-A--A-T----T-G--C-A---T--T--------------C--AA-A-A---C---------------T--G-C--TT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-T--A-----T-GG------AA-G-A--------------G-G-A-T---GG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC----CG-T--G--GC-GAA-G--G-C----G-----G--C-C-A-T---CTG-----G--TC-C-A------------------------------------------------------------------------------T-T------A-C-T--GA--CA---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0010233 M01867_242_000000000_ATWYL_1_2110_9820_4013 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-A--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-G-G--T-GGG----G--A-T--G---A----------------------------------------------T----------------------------GA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CC--------A-C-A-G---------A-A-----------T--AA-------------GC-T--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GGA------------------GCT-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TT-G-A-C----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--C-C-AG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-T-G-G-A--A-T----T-G--C-A---T--T--------------C--AA-G-A---C---------------T--G-T--CT--T-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--T-----C-GG------TA-G-A--------------G-G-T-G---AG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-T-C-A---CTG-----G--AC-C-G------------------------------------------------------------------------------A-C------A-T-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0021324 M01867_242_000000000_ATWYL_1_1109_19086_21969 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-A--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-G-G--T-GGG----G--A-C--G---A----------------------------------------------T----------------------------GA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CC--------A-C-A-G---------A-A-----------T--AA-------------GC-T--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GGA------------------GCT-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-TT-G-A-C----------------------AA----G-T-T-G------------G-A-T--G--TG--A-AA-GC--C-C-AG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-T-G-G-A--A-T----T-G--C-A---T--T--------------C--AA-G-A---C---------------T--G-T--CT--T-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--T-----C-GG------TA-G-A--------------G-G-T-G---AG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-C-A---CTG-----G--AC-C-G------------------------------------------------------------------------------A-C------A-T-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000486 M01867_242_000000000_ATWYL_1_1109_15574_27060 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-G--G--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TT-TT-G-T--C-AGG----G--A-A--G---AA--AAG-C---T-C-T--G-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CC---C-GGG-G-TG-A-T-G-A-C-G-----G-T---A-C-CT--------G-A-A-G---------A-A-----------T--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-G-C----------------------AA----G-A-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GT-G-A---C---------------T--G-C--AC--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-GG------TA-G-A--------------G-G-G-G---GA-T--------------GG--A--ATT-----------C-C-G-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------C-G---G-A--G-G-A-AC-A-CC---GAT----G--GC-GAA-G--G-C----A-----A--T-C-C-C---CTG-----G--AC-C-T------------------------------------------------------------------------------G-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000911 M01867_242_000000000_ATWYL_1_1102_13374_13655 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-G--G--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TT-TT-G-T--C-AGG----G--A-A--G---AA--AAG-C---T-C-T--G-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CC---T-GGG-G-TC-A-T-G-A-C-G-----G-T---A-C-CT--------G-A-A-G---------A-A-----------T--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-GA-T-G-C----------------------AA----G-A-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GT-G-A---C---------------T--G-C--AT--C-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--G-----C-GG------CA-G-A--------------G-G-G-G---GA-T--------------GG--A--ATT-----------C-C-G-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------C-G---G-A--G-G-A-AC-A-CC----GA-T--G--GC-GAA-G--G-C----A-----A--T-C-C-C---CTG-----G--GC-C-T------------------------------------------------------------------------------G-C------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002277 M01867_242_000000000_ATWYL_1_1105_16946_22257 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GG---------------------------GC-A--A--C-C--C-T-G-A-T-C-C-A---GC-C-----A--T-TCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-A-G--C-CGG----A--A-C--G---AA--ACG-G---T-A-C--G-C--T-------------------C--T----------------------------AA--------------------------------C---A----T---------------AG---C-GTG-C-TA-A-T-G-A-C-G-----G-T---A-C-CG--------G-C-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--A-TT-G-T-T----------------------AA----G-C-A-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-T-AA-----------C-C-T-G-G-G-A--A-T----G-G--C-A---T--T--------------T--TG-A-A---C---------------T--G-G--CA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-GT------CA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC---AAT----G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GA-T-G------------------------------------------------------------------------------A-C------A-C-T--GA--CG---C----------------------------T-C--A-TG--T-A-CG-A--AA-G-C----G-TG--GG-T--AG-C-A-AA--CA

Otu0000498 M01867_242_000000000_ATWYL_1_1113_11460_23942 .GGG--G-A-A--TC-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-C-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-GC--------------------------TT-TT-G-T--C-AGG----G--A-G--C---AA--ATC-T---T-T-G--A-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CC---T-CCG-A-GG-A-T-G-A-G-A-----G-T---A-C-CT--------G-A-A-G---------A-A-----------T--AA-------------GG-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------CCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-T-G---------------G--C-CC-G-T-T----------------------AA----G-A-G-T------------G-A-T--G--TG--A-AA-TC--C-C-CG-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-G-G-G-A--A-T----T-G--C-A---T--T--------------G--CT-A-A---C---------------T--G-G--CG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC---GAT----G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--CC-T-G------------------------------------------------------------------------------C-T------A-C-T--GA--CA---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000685 M01867_242_000000000_ATWYL_1_1101_24726_13695 .GGG--G-A-A--TC-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-C-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-GC--------------------------TT-TT-G-T--C-AGG----G--A-G--C---AA--ATC-C---T-T-G--T-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CC---A-CTG-G-GG-A-T-G-A-G-A-----G-T---A-C-CT--------G-A-A-G---------A-A-----------T--AA-------------GG-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------CCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-T-G---------------G--C-CC-G-T-T----------------------AA----G-A-G-T------------G-A-T--G--TG--A-AA-TC--C-C-CG-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-G-G-G-A--A-T----T-G--C-A---T--T--------------G--CT-A-A---C---------------T--G-G--CG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC---GAT----G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--CC-T-G------------------------------------------------------------------------------C-T------A-C-T--GA--CA---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004569 M01867_242_000000000_ATWYL_1_1104_13624_16756 .GGG--G-A-A--TC-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-C-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-GC--------------------------TT-TT-G-T--C-TGG----G--A-G--C---AA--ATC-C---T-T-G--T-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CC---A-CCG-G-GG-A-T-G-A-G-A-----G-T---A-C-CA--------G-A-A-G---------A-A-----------T--AA-------------GG-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------CCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-T-G---------------G--T-TC-G-T-T----------------------AA----G-A-G-T------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-T-AA-----------C-C-T-G-G-G-A--A-T----T-G--C-A---T--T--------------G--CT-A-A---C---------------T--G-G--CG--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-G-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------C-G---G-A--G-G-A-AC-A-CC---GAT----G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--CC-T-G------------------------------------------------------------------------------C-T------A-C-T--GA--CA---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0011993 M01867_242_000000000_ATWYL_1_1104_13719_4602 .GGG--G-A-A--TC-TT--GG-A-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-C-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-GC--------------------------TT-TT-G-T--C-TGG----G--A-G--C---AA--ATC-C---T-G-G--C-C--T-------------------C--T----------------------------AA--------------------------------T---A----C---------------AG---G-TCG-G-GG-A-T-G-A-G-A-----G-T---A-C-CA--------G-A-A-G---------A-A-----------T--AA-------------GG-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------CCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-CC-------G-CA-G-G-T-G---------------G--C-TT-G-A-T----------------------AA----G-A-T-A------------G-A-C--G--TG--A-AA-TC--C-C-TG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-A-G-G-A--A-T----T-G--C-G---T--T--------------T--AT-G-A---C---------------T--G-T--CT--C-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-GG------GA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC---GAT----G--GC-GAA-G--G-C----A-----A--C-C-C-C---CTG-----G--CC-T-A------------------------------------------------------------------------------A-T------A-C-T--GA--CA---C----------------------------T-C--A-TG--G-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000582 M01867_242_000000000_ATWYL_1_1101_11618_8878 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--T-TGG----G--A-A--G---AA--ATT-G---C-T-T--C-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CC---G-GAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CA--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-C--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000669 M01867_242_000000000_ATWYL_1_1112_21706_16232 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--T-TGG----G--A-A--G---AA--ATT-G---C-T-T--C-G--G-------------------A--T----------------------------AA--------------------------------T---A----C---------------CC---G-GAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CA--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-C--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000805 M01867_242_000000000_ATWYL_1_1108_28047_16275 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--C-GGG----G--A-A--G---AA--ATG-G---C-G-C--T-T--T-------------------T--T----------------------------AA--------------------------------T---A----C---------------AG---A-GCG-T-TG-A-T-G-A-C-G-----G-T---A-C-CC--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GG-G-A---C---------------T--G-C--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-AG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---GAT----G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GT-T-A------------------------------------------------------------------------------C-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001987 M01867_242_000000000_ATWYL_1_1114_9440_8672 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--C-GGG----G--A-A--G---AA--ATT-G---C-T-T--C-G--G-------------------C--T----------------------------AA--------------------------------T---A----T---------------CC---G-GAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CC--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-T--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-AG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GT-T-A------------------------------------------------------------------------------C-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004515 M01867_242_000000000_ATWYL_1_2110_8276_25279 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--T-TGG----G--A-A--G---AA--ATT-G---C-T-T--C-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CC---G-GAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CA--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-C--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---GAT----G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0007375 M01867_242_000000000_ATWYL_1_2109_17520_16137 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--T-TGG----G--A-A--G---AA--ATT-G---C-T-T--C-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CC---G-GAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CA--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-C--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-AG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---GAT----G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GT-T-A------------------------------------------------------------------------------C-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0010360 M01867_242_000000000_ATWYL_1_1104_14524_27081 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--T-GGG----G--A-A--G---AA--ATT-G---C-T-T--C-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CC---G-GAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CC--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-C--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0012224 M04041_72_000000000_AUMPE_1_1106_19324_20824 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--C-GGG----G--A-A--G---AA--ATG-G---C-G-C--T-T--T-------------------T--T----------------------------AA--------------------------------T---A----C---------------AG---A-GCG-T-TG-A-T-G-A-C-G-----G-G---A-C-CC--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGG------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GG-G-A---C---------------T--G-C--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-AG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---GAT----G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GT-T-A------------------------------------------------------------------------------C-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0013357 M01867_242_000000000_ATWYL_1_2108_14174_8727 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--C-GGG----G--A-A--G---AA--ATG-G---C-G-C--T-T--T-------------------T--T----------------------------AA--------------------------------T---A----C---------------AG---A-GCG-T-TG-A-T-G-A-C-G-----G-T---A-C-CC--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGG------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GG-G-A---C---------------T--G-C--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-AG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---GAT----G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GT-T-A------------------------------------------------------------------------------C-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0015964 M04041_72_000000000_AUMPE_1_2110_26615_6660 .GGG--G-A-A--TT-TT--CC-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--T-TGG----G--A-A--G---AA--ATT-G---C-T-T--C-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CC---G-GAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CA--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-C--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0019359 M01867_242_000000000_ATWYL_1_1109_25175_7398 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--C-GGG----G--A-A--G---AA--ATG-G---C-A-C--G-C--T-------------------C--T----------------------------AA--------------------------------C---A----T---------------AG---C-GTG-T-TG-A-T-G-A-C-G-----G-T---A-C-CC--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-C--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-AG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---GAT----G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GT-T-A------------------------------------------------------------------------------C-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0022389 M01867_242_000000000_ATWYL_1_2106_21442_14668 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--C-GGG----G--A-A--G---AA--ATG-G---G-G-C--G-T--T-------------------T--T----------------------------AA--------------------------------T---A----C---------------AG---A-GCG-T-TG-A-T-G-A-C-G-----G-T---A-C-CC--------G-C-C-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AA---GT-AG-GGG------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T-GTG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-G-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GG-G-A---C---------------T--G-C--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-AG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC---GAT----G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GT-T-A------------------------------------------------------------------------------C-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000050 M01867_242_000000000_ATWYL_1_1101_26894_6932 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--A-CGG----A--A-A--G---AA--ATC-G---C-A-C--G-G--A-------------------T--A----------------------------AA--------------------------------T---A----G---------------TC---T-GTG-T-GG-A-T-G-A-C-G-----G-T---A-C-CG--------T-A-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-C-A-T----------------------AA----G-A-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-G---T--T--------------T--GT-G-A---C---------------T--G-T--GC--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--A-----C-GG------TA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-TC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--AC-C-T------------------------------------------------------------------------------G-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002659 M04041_72_000000000_AUMPE_1_2105_2698_19485 .GGG--G-A-A--TT-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--A-CGG----A--A-A--G---AA--ATC-G---C-A-C--G-G--A-------------------T--A----------------------------AA--------------------------------T---A----G---------------TC---T-GTG-T-GG-A-T-G-A-C-G-----G-T---A-C-CG--------T-A-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-C-A-T----------------------AA----G-A-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-G---T--T--------------T--GT-G-A---C---------------T--G-T--GC--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--A-----C-GG------TA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-TC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--AC-C-T------------------------------------------------------------------------------G-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0018965 M01867_242_000000000_ATWYL_1_1109_5575_12109 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GG---------------------------GC-A--A--C-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--A-CGG----A--A-A--G---AA--ATC-G---C-A-C--G-G--A-------------------T--A----------------------------AA--------------------------------T---A----G---------------TC---T-GTG-T-GG-A-T-G-A-C-G-----G-T---A-C-CG--------T-A-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-C-A-T----------------------AA----G-A-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-G---T--T--------------T--GT-G-A---C---------------T--G-T--GC--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--A-----C-GG------TA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-TC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--AC-C-T------------------------------------------------------------------------------G-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0019141 M04041_72_000000000_AUMPE_1_2111_11471_26425 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--A-CGG----A--A-A--G---AA--ATC-G---C-A-C--G-G--A-------------------T--A----------------------------AA--------------------------------T---A----G---------------TC---T-GTG-T-GG-A-T-G-A-C-G-----G-T---A-C-CG--------T-A-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-C-A-T----------------------AA----G-A-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-G---T--T--------------T--GT-G-A---C---------------T--G-T--GC--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--A-----C-GG------TA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-TC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--AC-C-T------------------------------------------------------------------------------G-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0029503 M04041_72_000000000_AUMPE_1_2106_3855_9171 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-A--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--A-CGG----A--A-A--G---AA--ATC-G---C-A-C--G-G--A-------------------T--A----------------------------AA--------------------------------T---A----G---------------TC---T-GTG-T-GG-A-T-G-A-C-G-----G-T---A-C-CG--------T-A-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-C-A-T----------------------AA----G-A-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-G---T--T--------------T--GT-G-A---C---------------T--G-T--GC--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--A-----C-GG------TA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-TC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--AC-C-T------------------------------------------------------------------------------G-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000066 M01867_242_000000000_ATWYL_1_1101_25910_5979 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--T-GGG----G--A-A--G---AA--ATT-G---C-T-T--C-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CC---G-GAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CC--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-T--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001812 M01867_242_000000000_ATWYL_1_2103_10399_15924 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--C-GGG----G--A-A--G---AA--ATA-G---C-G-T--G-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CC---T-GCG-T-TG-A-T-G-A-C-G-----G-T---A-C-CC--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TA-T-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-T--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004429 M01867_242_000000000_ATWYL_1_1103_13982_23163 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--C-GGG----G--A-A--G---AA--ATT-G---C-A-T--T-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CC---G-GTG-T-AG-A-T-G-A-C-G-----G-T---A-C-CC--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-T--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

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Chapter 3

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Otu0004850 M01867_242_000000000_ATWYL_1_1104_8598_11872 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--T-GGG----G--A-A--G---AA--ATT-G---C-T-T--G-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CC---T-AAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CC--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TA-T-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-T--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0005045 M01867_242_000000000_ATWYL_1_1107_17939_8268 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--T-GGG----G--A-A--G---AA--ATT-G---C-T-C--A-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CT---T-GAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CC--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-T--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0011887 M01867_242_000000000_ATWYL_1_1109_23199_24634 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--T-TGG----G--A-A--G---AA--ATT-G---C-T-T--G-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CC---T-AAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CA--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-T--AC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0014966 M01867_242_000000000_ATWYL_1_2105_15210_11435 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--T-GGG----G--A-A--G---AA--ATT-G---C-T-T--C-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CC---G-GAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CC--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-T--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0014976 M01867_242_000000000_ATWYL_1_1104_13038_12677 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--T-TGG----G--A-A--G---AA--ATT-G---C-T-T--C-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CC---G-GAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CA--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-T--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0020270 M04041_72_000000000_AUMPE_1_2106_18368_8020 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-G--T-GGG----G--A-A--G---AA--ATT-G---C-T-T--C-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CC---G-GAG-T-AG-A-T-G-A-C-G-----G-T---A-C-CC--------G-C-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TG-T-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-G-A---C---------------T--G-T--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------GG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AC-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001025 M01867_242_000000000_ATWYL_1_1102_13374_13655 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-G--G--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TT-TT-G-T--C-AGG----G--A-A--G---AA--AAG-C---T-C-T--G-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CC---T-GGG-G-TC-A-T-G-A-C-G-----G-T---A-C-CT--------G-A-A-G---------A-A-----------T--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-GA-T-G-C----------------------AA----G-A-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GT-G-A---C---------------T--G-C--AT--C-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--G-----C-GG------CA-G-A--------------G-G-G-G---GA-T--------------GG--A--ATT-----------C-C-G-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------C-G---G-A--G-G-A-AC-A-CC----GA-T--G--GC-GAA-G--G-C----A-----A--T-C-C-C---CTG-----G--GC-C-T------------------------------------------------------------------------------G-C------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0006109 M01867_242_000000000_ATWYL_1_1103_8964_4587 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-C--G-AGG----G--A-A--G---AA--AAC-G---C-A-C--T-C--T-------------------C--T----------------------------AA--------------------------------T---A----C---------------GG---G-GTG-T-GG-A-T-G-A-C-G-----G-T---A-C-CT--------T-G-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TT-T-G-T----------------------AA----G-A-C-A------------G-G-C--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-T----G-G--C-G---T--T--------------T--GT-G-A---C---------------T--G-C--AA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0007481 M01867_242_000000000_ATWYL_1_2102_10930_13120 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-C--G-AGG----G--A-A--G---AA--AAT-G---C-A-C--T-C--T-------------------C--T----------------------------AA--------------------------------T---A----C---------------AG---G-GTG-T-AG-A-T-G-A-C-G-----G-T---A-C-CT--------T-G-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TT-G-T-T----------------------AA----G-A-C-A------------G-G-C--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-T----G-G--C-G---T--T--------------T--GT-G-A---C---------------T--G-G--CA--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0008702 M01867_242_000000000_ATWYL_1_1103_23103_9068 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-C--G-AGG----G--A-A--G---AA--AAC-G---C-A-C--T-G--G-------------------T--T----------------------------AA--------------------------------T---A----C---------------CT---G-GTG-T-GG-A-T-G-A-C-G-----G-T---A-C-CT--------T-G-A-T---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCG-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-TT-G-T-T----------------------AA----G-A-C-A------------G-G-C--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-G----G-T--C-G---T--T--------------T--GT-G-A---C---------------T--G-G--CA--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-GG------CA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TG-----------------------------T-G---G-A--G-G-A-AT-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--C-C-C-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004178 M01867_242_000000000_ATWYL_1_1108_9356_10845 .AGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--GCC----T-G-A-C-G-C-A---GC-A-----A--C-ACC-G-C-G-T-----G-A-G--T--G---------------------------A-T--G--AA-G-C-AT---------CT-TG-------G-T-G--------T-G-T--A---AA-A-C-TC--------------------------TG-TC-A-G--A-GGG----G--A-C--G---A-------------------------------------------A--T-----------------------------T--------------------------------T------------------------------------------T-G-A-C-G-----G-T---A-C-CC--------T-C-A-A---------A-G-----------G--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---GT-AG-GAT------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GT-TC-------G-TA-G-G-C-G---------------G--C-AT-G-T-C----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-GC--C-C-GG-A-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-G-G-T--T--C---G-G--C-A---T--T--------------G--GA-T-A---C---------------T--A-G--CA--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-AG------AA-G-A--------------G-G-C-A---AG-G--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GTA-A--G-C----G-----C--C-T-T-G---CTG-----G--TC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----C-AG--GG-G--AG-C-A-AA--TG

Otu0010716 M01867_242_000000000_ATWYL_1_1110_10894_19276 .AGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--GCC----T-G-A-C-G-C-A---GC-A-----A--C-ACC-G-C-G-T-----G-T-G--G--G---------------------------A-T--G--AC-G-C-TT---------TT-CG-------G-A-G--------T-G-T--A---AA-C-C-AC--------------------------TG-TC-A-G--A-GGG----G--A-C--G---A-------------------------------------------A-------------------------------AC--------------------------------C------------------------------------------T-G-A-C-G-----G-T---A-C-CC--------T-C-A-A---------A-G-----------G--AA-------------GC-A--TC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---GT-AG-GAT------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GT-TC-------G-TA-G-G-C-G---------------G--C-CT-G-T-C----------------------AA----G-T-C-T------------G-A-T--G--TT--A-AA-GC--C-C-GG-A-A-----------------------------------------------------CT-C-AA-----------T-T-C-C-G-G-T--T--C---A-G--C-A---T--T--------------G--GA-T-A---C---------------T--G-A--CA--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-AG------AA-G-A--------------G-G-C-A---AG-G--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-A--G-C----G-----C--C-T-T-G---CTG-----G--TC-T-G------------------------------------------------------------------------------A-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--G-A-CG-A--AA-G-C----C-AG--GG-G--AG-C-A-AA--TG

Otu0001198 M01867_242_000000000_ATWYL_1_1102_13374_13655 .GGG--G-A-A--TT-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-G--G--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TT-TT-G-T--C-AGG----G--A-A--G---AA--AAG-C---T-C-T--G-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CC---T-GGG-G-TC-A-T-G-A-C-G-----G-T---A-C-CT--------G-A-A-G---------A-A-----------T--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-GC-------G-CA-G-G-C-G---------------G--T-GA-T-G-C----------------------AA----G-A-C-A------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GT-G-A---C---------------T--G-C--AT--C-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--G-----C-GG------CA-G-A--------------G-G-G-G---GA-T--------------GG--A--ATT-----------C-C-G-C-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TG-----------------------------C-G---G-A--G-G-A-AC-A-CC----GA-T--G--GC-GAA-G--G-C----A-----A--T-C-C-C---CTG-----G--GC-C-T------------------------------------------------------------------------------G-C------A-C-T--GA--CG---C----------------------------T-C--A-TG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001531 M01867_242_000000000_ATWYL_1_1106_17417_27753 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CT---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-A-A--T-GGG----A--A-A--G---AA-GTGT-A---T-T-G--G-A--G-------------------C--T----------------------------AA--------------------------------T---A----T---------------CT---T-TGA-T-AC-T-T-G-A-C-G-----G-T---A-C-CC--------A-T-A-A---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-GG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TT-G-T-T----------------------AA----G-T-C-T------------G-T-T--G--TG--A-AA-GC--C-C-TG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-A-G-G-A--A-G----T-G--C-A---C--T--------------G--GA-T-A---C---------------T--G-G--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-GA-GT--G--------A-A-A---TC-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-G-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-T-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-T--AG-C-A-AA--CA

Otu0001585 M01867_242_000000000_ATWYL_1_2114_3424_20160 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CT---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-A-G--A-GGG----G--A-A--G---AA-ATGCA----T-A-G--A-T--G-------------------C--T----------------------------AA--------------------------------T---A----T---------------CA---T-CCT-T-GC-T-T-G-A-C-G-----G-T---A-C-CC--------T-C-A-A---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-CT-T-T-T----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-G-G-G-A--A-G----T-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-G--GA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-GA-GT--G--------A-A-A---TC-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-T-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002112 M01867_242_000000000_ATWYL_1_2112_12131_13421 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-A-G--G-GGG----G--A-A--G---AA-ATGCA----G-T-T--G-T--G-------------------C--T----------------------------AA--------------------------------T---A----T---------------CG---C-AAT-T-GC-T-T-G-A-C-G-----G-T---A-C-CC--------T-C-A-A---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TT-G-G-T----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-G----T-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-T--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-GA-GT--G--------A-A-A---TC-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-T-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002714 M01867_242_000000000_ATWYL_1_1104_17168_22108 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CT---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-A-A--T-GGG----A--A-A--G---AA-GTGT-A---T-T-G--G-A--G-------------------C--T----------------------------AA--------------------------------T---A----T---------------CT---C-CGA-T-AC-T-T-G-A-C-G-----G-T---A-C-CC--------A-T-A-A---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-GG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TT-G-T-T----------------------AA----G-T-C-T------------G-T-T--G--TG--A-AA-GC--C-C-TG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-A-G-G-A--A-G----T-G--C-A---C--T--------------G--GA-T-A---C---------------T--G-G--CA--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-GA-GT--G--------A-A-A---TC-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-T-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-T--AG-C-A-AA--CA

Otu0002949 M01867_242_000000000_ATWYL_1_1102_6873_17579 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CT---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-A-A--T-GGG----A--A-A--G---AA-GTGT-A---T-T-G--T-G--G-------------------C--T----------------------------AA--------------------------------T---A----T---------------CC---A-CGA-T-AC-T-T-G-A-C-G-----G-T---A-C-CC--------A-T-A-A---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-GG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TT-G-T-T----------------------AA----G-T-C-T------------G-T-T--G--TG--A-AA-GC--C-C-TG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-A-G-G-A--A-G----T-G--C-A---C--T--------------G--GA-T-A---C---------------T--G-G--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-GA-GT--G--------A-A-A---TC-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-T-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-T--AG-C-A-AA--CA

Otu0004964 M01867_242_000000000_ATWYL_1_1108_11362_20791 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CT---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-A-G--A-GGG----G--A-A--G---AA-ATGA-----A-A-G--G-T--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CA---T-CTT-T-TC-T-T-G-A-C-G-----G-T---A-C-CC--------T-C-A-A---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-CT-T-T-T----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-G-G-G-A--A-G----T-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-G--GA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-GA-GT--G--------A-A-A---TC-C-GT-AG--AT-A-TT-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-T-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0006925 M01867_242_000000000_ATWYL_1_2103_18387_13012 .GGG--G-A-A--TT-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CT---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TC-A-A--T-GGG----A--A-A--G---AA-GTGT-A---T-T-G--C-A--G-------------------C--T----------------------------AA--------------------------------T---A----T---------------CT---G-CGA-T-AC-T-T-G-A-C-G-----G-T---A-C-CC--------A-T-A-A---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-GG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--T-TT-G-T-T----------------------AA----G-T-C-T------------G-T-T--G--TG--A-AA-GC--C-C-TG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-A-G-G-A--A-G----T-G--C-A---C--T--------------G--GA-T-A---C---------------T--G-G--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-T--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-GA-GT--G--------A-A-A---TC-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-T-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-T--AG-C-A-AA--CA

Otu0007856 M01867_242_000000000_ATWYL_1_1111_4957_13051 .GGG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--AC---------------------------GC-A--A--GT---C-T-G-A-C-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-TC--------------------------TG-TG-G-A--G-AGG----G--A-CGAAAAGGACCTGG-------------------------------------C--CA---------------------------AT-------------------------------------------------------------------------A-TCC-AGG-GA---GATTG--ACGGTACC------TCT-T-T---------A-G-----------C--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---CG-----------------G--TA-AT-------AC---GG-AG-GGT------------------GCA-A-A------------C--G--T---T--GC-T-CGG-AA------TC-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GC-------G-TA-G-G-C-G---------------G--C-TC-G-C-T----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-GC--C-C-TC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-A-G-G-A--A-G----T-G--C-A---T--T--------------C--GA-A-A---C---------------T--G-A--CG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--A-----T-GG------AA-G-A--------------G-G-G-T---CG-C--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-G-A-C---CTG-----G--GC-C-A------------------------------------------------------------------------------A-T------A-T-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0005126 M01867_242_000000000_ATWYL_1_1108_8806_9375 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TG-TC-A-G--G-TGG----G--A-A--G---AA-GGTG-C---G-T-T--T-G--G-------------------G--T----------------------------AA------------------------------------C----T---------------AC-----GGG-CGTA-C-T-G-A-C-G-----G-T---A-C-CG--------C-C-G-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-TT-G-T-C----------------------AA----G-T-C-A------------G-G-T--G--TG--A-AA-GC--C-C-GC-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-G-G-A--A-T----T-G--C-G---C--T--------------T--GA-A-A---C---------------T--G-G--CA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-C--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0007770 M01867_242_000000000_ATWYL_1_1103_21375_17508 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GG---------------------------GC-A--A--C-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-AC------------------------TGTCGAGTGGAACGAGCCGGC-G--G-AGGT---GA-ATAT-C---C-------------------------------T--C----------------------------CT------------------------------------------------------------------G-C-CC---C-G-A-C-G-----G-T---A-C-CA--------C-T-T-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-GC-------G-CA-G-G-C-G---------------G--G-CT-G-T-T----------------------AA----G-T-C-T------------G-G-T--G--TG--A-AA-TC--C-C-TC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-A-G-G-A--C-G----T-G--C-A---T--C--------------G--GA-T-A---C---------------T--G-G--CA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--C-----C-TG------GA-G-A--------------G-G-G-T---GG-C--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--C-C-A-C---CTG-----G--AC-A-G------------------------------------------------------------------------------T-G------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----A-TG--GG-T--AG-C-A-AA--CA

Otu0009452 M01867_242_000000000_ATWYL_1_1112_10142_5699 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-C-A---GC-C-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-CC--------------------------TG-TCGTGAGGGAAGAA--AACCCTTCATTAGA-------------------------------------------AG-G-G--------------------------TC---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------T-G-C-A---------A-G-----------A--AA-------------GC-G--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGC------------------GCA-A-G------------C--G--T---T--GC-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-TT-G-T-T----------------------AA----G-T-C-G------------G-G-T--G--TG--A-AA-GC--C-C-TG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-A-G-G-A--T-G----T-G--C-A---C--T--------------C--GA-A-A---C---------------T--G-G--CT--C-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-A---AG-C--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-C---CTG-----G--CC-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0009567 M01867_242_000000000_ATWYL_1_2106_5783_14398 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TG-TC-A-G--G-AGG----G--A-A--G---AA-GG-T-G---C-G-G--T-G--A-------------------A--G----------------------------TA--------------------------------A---C----T---------------GT---C-ATC-G-TA-C-T-G-A-C-G-----G-T---A-C-CT--------C-C-G-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-CT-A-T-C----------------------AA----G-T-C-A------------G-G-T--G--TG--A-AA-GC--C-T-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-A-G-A--A-T----T-G--C-G---C--T--------------T--GA-A-A---C---------------T--G-A--CA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-C--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0010173 M01867_242_000000000_ATWYL_1_2102_7004_19785 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TG-TC-A-G--G-CGG----G--A-A--G---AA-GGTG-C---G-G-T--C-G--G-------------------G--T----------------------------AA------------------------------------C----T---------------AC---G-GAC-G-TA-C-T-G-A-C-G-----G-T---A-C-CG--------C-C-G-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-TT-G-T-C----------------------AA----G-T-C-A------------G-G-T--G--TG--A-AA-GC--C-C-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-G-G-A--A-T----T-G--C-G---C--T--------------T--GA-A-A---C---------------T--G-G--CA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-C--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0012797 M01867_242_000000000_ATWYL_1_1103_9130_15072 .GAG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TG-TC-G-G--G-TGG------------G---AC-GATG-----T-G-A--C-T--C-------------------G--G----------------------------GT--------------------------------A---A----C---------------GA---C-CGG-G-TT-G-T-G-A-C-G-----G-T---A-C-CA--------T-C-A-A---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-GT-------G-TA-G-G-C-G---------------G--C-CT-G-T-T----------------------AT----G-T-C-G------------G-G-T--G--TG--A-AA-TC--C-C-AC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-T-G-G-A--A-C----G-G--C-G---C--T--------------C--GA-A-A---C---------------T--G-G--CA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-GG------AA-G-G--------------G-G-A-A---GG-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--A-G-A-AT-A-CC----GG-T--G--GC-GAA-G--G-C----G-----A--C-C-T-T---CTG-----G--CC-C-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0015073 M01867_242_000000000_ATWYL_1_1114_25795_19046 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-AC--------------------------TG-TC-A-G--G-AGA----G--A-A--G---AA-GGTG-C---G-G-A--C-G--G-------------------G--T----------------------------AA------------------------------------C----T---------------AC---G-TCC-G-TA-C-T-G-A-C-G-----G-T---A-C-CT--------C-C-G-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-CT-G-T-C----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-GC--C-C-GC-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-C-G-G-A--A-C----T-G--C-G---T--T--------------C--GA-A-A---C---------------T--G-G--CA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-C--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0017042 M01867_242_000000000_ATWYL_1_2109_17868_23120 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TG-TC-G-G--G-CGG----A--A-A--G---AA-GGTG-C---G-T-T--C-G--G-------------------G--T----------------------------AA------------------------------------C----T---------------AC-----GGG-CGTA-C-T-G-A-C-G-----G-T---A-C-CG--------C-C-G-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-TT-G-T-C----------------------AA----G-T-C-A------------G-G-T--G--TG--A-AA-TC--C-T-GT-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-A-G-A--A-T----T-G--C-G---C--T--------------T--GA-A-A---C---------------T--G-G--CA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-C--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000567 M01867_242_000000000_ATWYL_1_1112_13329_5236 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--AG---------------------------GA-A--A--C-T--C-T-G-A-T-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--C--G-C-AT---------TT-CG-------G-T-G--------T-G-T--A---AA-C-T-CC--------------------------TT-TT-A-T--A-AGG----G--A-A--G---A----------------------------------------------T----------------------------AA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------T-A-T-G---------A-A-----------T--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--AC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-AT-GT-------G-TA-G-G-C-T---------------G--T-AC-T-A-T----------------------AA----G-T-C-A------------G-G-A--G--TG--A-AA-TC--C-A-AC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-T-T-G-A--A-C----T-G--C-T---C--T--------------T--GA-A-A---C---------------T--G-T--TG--T-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--A-----T-GG------GA-G-A--------------G-G-T-A---GA-T--------------GG--A--ATT-----------G-G-T-G-GT--GT-A-G-GG-GT--A--------A-A-A---TC-C-GT-AG--AT-A-TC-----------------------------A-C---C-A--G-G-A-AT-A-CC----GA-T--T--GC-GAA-G--G-C----G-----A--T-C-T-A---CTG-----G--AA-C-A------------------------------------------------------------------------------T-T------A-T-T--GA--CG---C----------------------------T-G--A-GA--C-A-TG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0001841 M01867_242_000000000_ATWYL_1_1101_20264_14548 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-G-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-T-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TC-A-G--C-GAG----G--A-G--G---AA--AGG-T---T-G-G--T-A--G-------------------C--T----------------------------AA--------------------------------T---A----A---------------CT---G-CCA-G-CT-G-T-G-A-C-G-----T-T---A-C-TC--------G-C-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-TG-G-A-T----------------------AA----G-T-T-A------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-T----T-G--C-A---T--T--------------T--AA-A-A---C---------------T--G-T--CC--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----T-TG------TA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------T-G---G-A--G-G-A-AT-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-C-C-C---CTG-----G--AC-A-A------------------------------------------------------------------------------A-G------A-C-T--GA--CG---C----------------------------T-C--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0003479 M01867_242_000000000_ATWYL_1_1103_10148_14791 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-G-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-T-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TC-A-G--C-GAG----G--A-G--G---AA--AGG-T---G-G-C--T-A--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CT---A-GTT-G-CT-G-T-G-A-C-G-----T-T---A-C-TC--------G-C-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-CA-G-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-A-A---C---------------T--G-T--CT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----T-TG------TA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------T-G---G-A--G-G-A-AT-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-C-C-C---CTG-----G--AC-A-A------------------------------------------------------------------------------A-G------A-C-T--GA--CG---C----------------------------T-C--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0006024 M01867_242_000000000_ATWYL_1_1102_25957_14972 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-G-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-T-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TC-A-G--C-GAG----G--A-G--G---AA--AGG-T---G-G-C--T-A--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CT---A-GTT-G-CT-G-T-G-A-C-G-----T-T---A-C-TC--------G-C-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-TA-T-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-A-A---C---------------T--G-T--GT--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----T-TG------TA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------T-G---G-A--G-G-A-AT-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-C-C-C---CTG-----G--AC-A-A------------------------------------------------------------------------------A-G------A-C-T--GA--CG---C----------------------------T-C--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0008654 M01867_242_000000000_ATWYL_1_2105_11768_14260 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-G-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-T-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TC-A-G--C-GAG----G--A-G--G---AA--AGG-T---T-G-G--C-A--G-------------------C--T----------------------------AA--------------------------------T---A----T---------------CT---G-TCA-G-CT-G-T-G-A-C-G-----T-T---A-C-TC--------G-C-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-TG-G-A-T----------------------AA----G-T-T-A------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-T----T-G--C-A---T--T--------------T--AA-A-A---C---------------T--G-T--TC--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----T-TG------TA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------T-G---G-A--G-G-A-AT-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-C-C---CTG-----G--AC-A-A------------------------------------------------------------------------------A-G------A-C-T--GA--CG---C----------------------------T-C--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0005855 M01867_242_000000000_ATWYL_1_1104_11585_11460 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-G-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-T-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TC-A-G--C-GAG----G--A-G--G---AA--AGG-G---G-G-A--T-A--G-------------------C--T----------------------------AA--------------------------------T---A----T---------------CT---A-TTC-T-CT-G-T-G-A-C-G-----T-T---A-C-TC--------G-C-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-TA-G-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-A-A---C---------------T--G-T--CT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----T-TG------TA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------T-G---G-A--G-G-A-AT-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-C-C-C---CTG-----G--AC-A-A------------------------------------------------------------------------------A-G------A-C-T--GA--CG---C----------------------------T-C--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000948 M01867_242_000000000_ATWYL_1_1101_10247_14094 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-G-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-T-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TC-A-G--C-GAG----G--A-G--G---AA--AGG-G---G-G-A--T-A--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CT---A-TTC-T-CT-G-T-G-A-C-G-----T-T---A-C-TC--------G-C-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-CA-G-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-A-A---C---------------T--G-T--CT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----T-TG------TA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------T-G---G-A--G-G-A-AT-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-C-C---CTG-----G--AC-A-A------------------------------------------------------------------------------A-G------A-C-T--GA--CG---C----------------------------T-C--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0000959 M01867_242_000000000_ATWYL_1_2105_13285_22560 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-G-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-T-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TC-A-G--C-GAG----G--A-G--G---AA--AGG-G---T-G-G--C-G--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CT---G-TCA-T-CT-G-T-G-A-C-G-----T-T---A-C-TC--------G-C-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-TC-G-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-A-A---C---------------T--G-T--CG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----T-TG------TA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------T-G---G-A--G-G-A-AT-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-C-C---CTG-----G--AC-A-A------------------------------------------------------------------------------A-G------A-C-T--GA--CG---C----------------------------T-C--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002822 M01867_242_000000000_ATWYL_1_1101_22341_17936 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-G-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-T-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TC-A-G--C-GAG----G--A-G--G---AA--AGG-G---T-G-G--A-A--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CT---T-TCA-T-CT-G-T-G-A-C-G-----T-T---A-C-TC--------G-C-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-CA-G-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-A-A---C---------------T--G-T--CT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----T-TG------TA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------T-G---G-A--G-G-A-AT-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-C-C---CTG-----G--AC-A-A------------------------------------------------------------------------------A-G------A-C-T--GA--CG---C----------------------------T-C--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0003724 M01867_242_000000000_ATWYL_1_1101_19174_12034 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-G-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-T-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TC-A-G--C-GAG----G--A-G--G---AA--AGG-G---G-T-G--A-T--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CA---T-CAT-C-CT-G-T-G-A-C-G-----T-T---A-C-TC--------G-C-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-CA-G-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-A-A---C---------------T--G-T--CT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----T-TG------TA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------T-G---G-A--G-G-A-AT-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-C-C---CTG-----G--AC-A-A------------------------------------------------------------------------------A-G------A-C-T--GA--CG---C----------------------------T-C--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0013041 M01867_242_000000000_ATWYL_1_1105_5683_9980 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-G-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-T-G--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TT-TC-A-G--C-GAG----G--A-G--G---AA--AGG-G---G-G-A--T-A--G-------------------C--T----------------------------AA--------------------------------T---A----C---------------CT---A-TTC-T-CT-G-T-G-A-C-G-----T-T---A-C-TC--------G-C-A-G---------A-A-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--AA-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-TA-T-A-T----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------T--GA-A-A---C---------------T--G-T--GT--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----T-TG------TA-G-A--------------G-G-G-G---GG-T--------------AG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AG-A-TC-----------------------------T-G---G-A--G-G-A-AT-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--C-C-C-C---CTG-----G--AC-A-A------------------------------------------------------------------------------A-G------A-C-T--GA--CG---C----------------------------T-C--A-GG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0007037 M01867_242_000000000_ATWYL_1_2104_9305_10338 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-A-C-AC--------------------------TG-TC-A-G--G-AGG----A--A-A--G---AA-GGTT-C---C-G-G--C-T--A-------------------G--T----------------------------AA------------------------------------C----T---------------GC---G-TCG-G-AA-C-T-G-A-C-G-----G-T---A-C-CT--------C-C-G-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-TT-G-C-C----------------------AA----G-T-C-A------------G-G-T--G--TG--A-AA-TC--C-C-GT-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-G-G-A--A-C----T-G--C-G---C--C--------------T--GA-A-A---C---------------T--G-G--TA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-C--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0009443 M01867_242_000000000_ATWYL_1_1113_17332_16717 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-C-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-CC--------------------------TG-TC-G-G--GAGGGAA--GA-AAGCCTGCAACCGC---------------------------------------A--G-G--------------------------TC---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------C-G-C-A---------A-G-----------A--AA-------------GC-G--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGC------------------GCA-A-G------------C--G--T---T--GC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-CT-G-A-T----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TC--C-C-CA-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-A---T--T--------------C--GA-A-A---C---------------T--G-C--CA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-A---AG-C--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-C---CTG-----G--CT-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0010147 M01867_242_000000000_ATWYL_1_2101_18782_22229 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TG-TG-A-G--G-AGG----A--A-A--G---AA--CG--G---G-T-G--G-T--C-------------------G--G----------------------------GT--------------------------------A---A----C---------------TA---C-GGC-TGCC-T-T-G-A-C-G-----G-T---A-C-CT--------C-C-A-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-TC-T-C-T----------------------AA----G-T-C-G------------G-G-T--G--TG--A-AA-TC--C-C-TG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-G-G-G-A--A-C----T-G--C-G---C--T--------------C--GA-A-A---C---------------T--G-G--AG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-C--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC----AG-T--T--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0010682 M01867_242_000000000_ATWYL_1_1105_5804_14160 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--T--GA--------------------------T-G--A--A--G-G-CC---------CT-CG-------G-G-T--------C-G-T--A---AA-G-C-AC--------------------------TG-TC-A-G--G-AGG----A--A-A--G---AA-GGCT-G---C-A-G--T-G--A-------------------G--T----------------------------AA------------------------------------C----T---------------GC-----TCT-GCAG-C-T-G-A-C-G-----G-T---A-C-CT--------C-C-G-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-TC-G-G-A----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-GC-A-G-----------------------------------------------------CT-C-AA-----------C-T-G-C-G-G-A--A-C----T-G--C-G---T--C--------------T--GA-A-A---C---------------T--A-T--CG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-A-T---AG-C--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-T---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0011313 M01867_242_000000000_ATWYL_1_1113_16471_9954 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-C-----A--C-GCC-G-C-G-T-----G-T-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TG-TC-G-G--G-AGG----G--A-A--GA--A-------------------------------------------AGCACTTCGG---------------------TGCT--------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------C-G-C-A---------A-G-----------A--AA-------------GC-G--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGC------------------GCA-A-G------------C--G--T---T--GC-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--C-TG-T-C-T----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-T-T-G-G-G-A--A-C----T-G--C-A---T--T--------------C--GA-A-A---C---------------T--G-A--AC--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-T--A-----C-GT------GA-G-A--------------G-G-G-A---AG-C--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-A--G-C----G-----G--C-T-T-C---CTG-----G--CG-C-G------------------------------------------------------------------------------T-C------A-C-T--GA--CG---C----------------------------T-G--A-TG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0013030 M01867_242_000000000_ATWYL_1_1105_20277_20426 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-C-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-CC--------------------------TG-TC-G-G--GAGGGAC--GAAAGCC-GA--A-------------------------------------------A--G-G--------------------------TT---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------C-G-C-A---------A-G-----------A--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGT------------------GCA-A-G------------C--G--T---T--GC-T-CGG-AG------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--C-GA-A-A-T----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--C-G----T-G--C-A---T--T--------------C--GA-A-A---C---------------T--G-T--TT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-G--T-----C-GG------AA-G-A--------------G-G-G-A---AG-C--------------AG--A--ATT-----------C-C-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-A--G-C----G-----G--C-T-T-C---CTG-----G--TC-C-G------------------------------------------------------------------------------A-T------T-C-T--GA--CG---C----------------------------T-C--A-TG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0013361 M01867_242_000000000_ATWYL_1_1107_22221_16688 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TG-TC-A-G--G-AGG----A--A-A--G---AA-GGCT-G---T-T-G--T-G--A-------------------G--T----------------------------AA------------------------------------C----T---------------GC-----TCA-GCAG-C-T-G-A-C-G-----G-T---A-C-CT--------C-C-G-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-T---------------G--C-TC-T-G-C----------------------AA----G-T-C-A------------G-G-T--G--TG--A-AA-TC--C-C-GC-A-G-----------------------------------------------------CT-C-AA-----------C-T-G-C-G-G-A--A-C----T-G--C-G---C--C--------------T--GA-A-A---C---------------T--G-C--AG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-C--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0013605 M01867_242_000000000_ATWYL_1_1102_10536_24717 .GGG--G-A-A--TA-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-A-----A--C-GCC-G-C-G-T-----G-T-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-AC--------------------------TG-TG-A-G--G-AGG----A--A-A--G---AA-CGGG-T---G-G-T--C-G--G-------------------G--T----------------------------AA--------------------------------C---T----A---------------C----G-GCC-G-CT-T-T-G-A-C-G-----G-T---A-C-CT--------C-C-A-G---------A-G-----------G--AA-------------GC-A--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGT------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-TC-T-C-T----------------------AA----G-T-C-G------------G-G-T--G--TG--A-AA-TC--C-C-TG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-G-G-G-A--A-C----T-G--C-G---C--T--------------C--GA-A-A---C---------------T--G-G--AG--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-G-T---AG-C--------------GG--A--ATT-----------C-C-G-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------C-G---G-A--G-G-A-AC-A-CC---AGT----T--GC-GAA-G--G-C----G-----G--C-T-A-C---CTG-----G--AC-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0003768 M01867_242_000000000_ATWYL_1_1101_19489_7621 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GTC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-A-T--C-GAT----G--A-G--G---AA-TAAG-C---T-T-T--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-AGA-G-TG-A-T-G-A-C-G-----G-T---A-G-TC--------G-G-T-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-AT-------G-CA-G-G-C-G---------------G--T-TC-A-G-C----------------------AA----G-T-C-T------------G-G-T--G--TG--T-AA-GA--C-C-TC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-G-G--A-G----C-G--C-G---C--T--------------G--GA-A-A---C---------------T--G-T--TG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-AG------GA-A-G--------------G-G-G-A---GT-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC---AGT----G--GC-GAA-G--G-C----G-----A--A-C-T-C---CTG-----G--CC-A-T------------------------------------------------------------------------------G-T------A-C-T--GA--CG---C----------------------------T-G--A-GA--T-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004440 M01867_242_000000000_ATWYL_1_1101_17655_16253 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GTC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-A-T--A-GAC----G--A-G--G---AA-TAAG-C---T-G-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CTG-G-TG-A-T-G-A-C-G-----G-T---A-G-TC--------T-G-T-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-AT-------G-CA-G-G-C-G---------------G--T-TC-A-G-C----------------------AA----G-T-C-T------------G-G-T--G--TG--T-AA-GA--C-C-TC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-G-G--A-G----C-G--C-G---C--T--------------G--GA-A-A---C---------------T--G-T--TG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-AG------GA-A-G--------------G-G-G-A---GT-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC---AGT----G--GC-GAA-G--G-C----G-----A--A-C-T-C---CTG-----G--CC-A-T------------------------------------------------------------------------------G-T------A-C-T--GA--CG---C----------------------------T-G--A-GA--T-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0002856 M01867_242_000000000_ATWYL_1_2105_19148_24123 .CGA--G-G-A--TC-AT--TC-G-C------AA-T-G-C--GC---------------------------GA-A--A--G-C--G-T-G-A-C-G-G-T---GC-G-----A--C-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TG-TC-A-T--G-AGG----G--A-A--C---AA-TGTC-C---G-G-G--A-T--G-------------------T--T----------------------------AA--------------------------------C---A----C---------------CA---TCTCG---GG-G-T-G-A-T-A-----G-T---A-C-CT--------C-A-A-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GCG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-TC-T-G-T----------------------GT----G-T-C-G------------G-A-C--G--TG--A-AA-TC--T-C-AC-A-G-----------------------------------------------------CT-C-AA-----------C-T-G-T-G-G-A--A-T----T-G--C-A---T--T--------------T--GA-A-A---C---------------T--A-C--AG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--A-----C-AG------GA-G-A--------------G-G-A-G---AG-C--------------GG--A--ATT-----------C-T-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-A---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-C-T---CTG-----G--AC-T-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----T-GG--GG-G--AG-C-A-AA--CA

Otu0000683 M01867_242_000000000_ATWYL_1_1102_3673_10450 .TAA--G-A-A--TC-TT--CC-A-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-T-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-C-CC--------------------------TT-TT-A-T--A-GGC----G--A-G--G---AA-TAAG-A---T-C-C--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-GGG-T-CG-A-T-G-A-C-G-----G-T---A-G-CC--------T-G-T-GA----------A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AT-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-C-G---------------G--T-TC-G-A-C----------------------AA----G-T-C-T------------G-G-T--G--TT--A-AA-GC--C-C-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-C-G-G-A--A-A----G-G--C-A---T--T--------------G--GA-A-A---C---------------T--G-T--CG--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-TG------GA-G-G--------------G-G-T-A---GC-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-TC---AAT----G--GC-GAA-G--G-C----A-----A--G-C-T-A---CTG-----G--CC-A-T------------------------------------------------------------------------------G-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-T----A-TG--GG-G--AG-C-A-AA--CA

Otu0000961 M01867_242_000000000_ATWYL_1_1102_22651_23148 .GAG--G-A-A--TC-TT--TC-A-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-G-G-A---GC-G-----A--C-ACC-G-C-G-T-----G-G-A--G--GA--------------------------C-G--A--A--G-G-GT---------TT-AT-------T-C-T--------T-G-T--A---AA-C-T-CC--------------------------TT-TT-C-T--G-TGG----G--A-A--G---A----------------------------------------------T----------------------------AA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CA--------C-A-G-G---------A-A-----------T--AA-------------GG-G--AC-G---G-C-AAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TG-AT-------AC---GT-AG-GTC------------------CCG-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-CT-------G-TA-G-G-C-G---------------G--G-AG-A-A-T----------------------AC----G-T-C-A------------G-G-T--A--TA--A-AA-GG--A-A-GA-G-G-----------------------------------------------------CT-C-AA-----------C---C-T-C-T-T--T-A----T-G--T-G---C--T--------------T--GA-A-A---C---------------G--G-C--TT--T-C-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--G-----A-GG------GA-G-G--------------G-G-T-A---AG-C--------------AG--A--ATT-----------A-T-G-C-GA--GT-A-G-GG-GT--G--------C-A-A---TC-C-GT-TG--AT-A-CG-----------------------------C-A---T-A--A-G-A-AT-A-CC----AA-A--A--GC-GAA-G--G-C----A-----G--C-T-T-A---CTG-----G--AA-C-T------------------------------------------------------------------------------C-T------A-T-T--GA--CG---C----------------------------T-G--A-GA--G-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--AA

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Chapter 3

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Otu0001026 M01867_242_000000000_ATWYL_1_2102_11051_15395 .TCG--G-A-A--TA-TT--CG-G-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-C-C-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-C-G--G--GA--------------------------T-G--A--A--G-G-CC---------CTCTG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TG-TC-A-C--G-AGT----G--A-G--C---AA-ATTG-A---C-G-T--G-T--T----------------------T----------------------------TA--------------------------------A----------------------CAAA---A-TAC-GGCA-T-T-G-A-G-T-----T-A---G-G-CT--------CGA---G---------A-G-----------G--AA-------------GC-C--CC-C---G-C-TAA-----------------------T--C-A-C--G-T---------G-C-CA--G-C---A--G-C--GG---C-----------------GG--TA-AT-------AC---GT-GA-GGG------------------GCA-A-A------------C--G--T---T--GT-T-CGG-TG------TC-A--C-T--GG-GC-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-T-G---------------G--T-TT-C-G-T----------------------AA----G-T-A-A------------G-G-T--G--TG--A-AA-GC--C-C-CC-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-G-A--A-C----T-G--C-G---C--C--------------T--TA-T-A---C---------------T--G-C--GT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--A-----T-TA------CA-G-G--------------G-G-A-A---GG-G--------------GG--A--ACT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GC-AG--AT-A-TC-----------------------------T-G---G-G--G-G-A-AG-G-CC---GGC----G--GC-GAA-G--G-C----G-----C--C-C-T-T---CTG-----G--GT-A-A------------------------------------------------------------------------------T-T------C-C-T--GA--CA---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0003286 M01867_242_000000000_ATWYL_1_1101_13114_5309 .GGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GG---------------------------GC-A--A--C-C--C-T-G-A-T-C-C-A---GC-A-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-AG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TC-G-C--A-CGC----G--A-C--G---A----------------------------------------------T----------------------------GA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-G-CG--------T-G-A-G---------A-A-----------G--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-CT-G-A-T----------------------GA----G-T-T-A------------G-A-A--G--TG--A-AA-GC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-G-G-A--A-C----T-G--C-T---T--T--------------T--AA-T-A---C---------------T--G-T--CG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----T-CG------GA-G-G--------------G-G-A-T---GG-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC---GGC----G--GC-GAA-G--G-C----G-----G--C-C-A-T---CTG-----G--AC-G-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0003382 M01867_242_000000000_ATWYL_1_1106_11042_24790 .AGG--G-A-A--TA-TT--GG-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--C--GA--------------------------T-G--A--C--G-G-TC---------CT-CGA------G-A-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TT-C-T--G-AGG----A--A-A--G---AA-CATC-C---G-G-G--G-T--A-G--------------------G----------------------------AA--------------------------------A----------------------T-GA---C-CCC-G-GC-T-T-G-A-C-G-----G-T---A-C-CT--------C-A-G-G---------A-A-----------T--AA-------------GG-A--AC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GTT------------------CCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GA-GC-------G-TA-G-G-C-G---------------G--A-TA-G-G-T----------------------CA----G-T-C-T------------A-A-A--G--TG--G-AA-TC--C-C-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-C-G-G-A--C-T----T-G--C-T---T--C--------------G--GA-T-A---C---------------T--G-C--CT--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-GT------GA-T-A--------------G-G-A-A---GA-T--------------GG--A--ATT-----------C-A-G-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-AC-AG--AT-A-TC-----------------------------C-T---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-A--G-C----G-----G--T-C-T-T---CTG-----G--CG-C-G------------------------------------------------------------------------------A-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-T-CG-A--AA-G-C----G-TG--GG-T--AG-C-A-AA--CG

Otu0003411 M01867_242_000000000_ATWYL_1_1102_15821_6814 .AGG--G-A-A--TA-TT--GG-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-C-C-A---GC-C-----A--T-GCC-G-C-G-T-----G-A-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-C-TC--------------------------TT-TT-C-T--G-AGG----G--A-A--G---AA--CAACC---G-G-A--A-C--A-------------------G--G----------------------------AA--------------------------------A--------T---------------GG---T-TCC-G-GT-C-T-G-A-C-G-----G-T---A-C-CT--------C-A-G-G---------A-A-----------T--AA-------------GG-G--AC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GTC------------------CCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GA-GC-------G-CA-G-G-C-G---------------G--A-CA-G-G-T----------------------CA----G-T-C-T------------G-A-A--G--TG--G-AA-AT--C-C-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-C-G-G-A--C-T----T-G--C-T---T--C--------------G--GA-T-A---C---------------T--G-C--CT--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-GT------GA-G-A--------------G-G-A-A---GA-T--------------GG--A--ATT-----------C-A-G-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-TC-AG--AT-A-TC-----------------------------C-T---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-A--G-C----G-----G--T-C-T-T---CTG-----G--CG-C-G------------------------------------------------------------------------------A-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-T-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CG

Otu0003472 M01867_242_000000000_ATWYL_1_1102_6882_11429 .GAG--G-A-A--TC-TT--CC-A-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-T-G-G-A---GC-G-----A--C-ACC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-CC---------TA-AC-------G-G-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-C-T--G-AGG----G--A-G--C---A----------------------------------------------T----------------------------AA---------------------------------------------------------------------------T-G-A-T-A-----G-T---A-T-CT--------C-A-G-G---------A-A-----------T--AA-------------GA-G--GC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GCC------------------TCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GT-CT-------G-TA-G-G-C-G---------------G--T-TA-A-T-T----------------------AA----G-T-C-A------------G-G-T--A--TG--A-AA-GA--C-C-GG-A-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-G-T-G--T-T----T-G--T-G---C--T--------------T--GA-A-A---C---------------T--G-G--TT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--T-----A-GG------GA-G-A--------------G-G-T-T---AG-C--------------AG--A--ATT-----------C-T-A-A-GT--GT-A-G-GG-GT--G--------C-A-A---TC-C-GT-AG--AT-A-CT-----------------------------T-A---G-A--G-G-A-AT-A-CC----AA-A--A--GC-GAA-G--G-C----A-----G--C-T-A-A---CTG-----G--AA-C-T------------------------------------------------------------------------------A-T------A-T-T--GA--CG---C----------------------------T-G--A-AA--G-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--AA

Otu0003733 M01867_242_000000000_ATWYL_1_1105_11635_3112 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--C--GA--------------------------T-G--A--A--G-GTTC---------TC-TG-------A-A-T--------T-G-T--A---AA-G-T-CC--------------------------TT-TA-G-G--G-CAG----G--A-A--G---AA-TAAG-T---G-C-G--C-G----------------------C--C----------------------------AA--------------------------------T---A----C----------------C---G-CGT-A-CG-A-T-G-A-C-G-----A-C---C-CTGC--------C-T-A-A---------AT---------------AA-------------GC-T--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--CA-AT-------AC---GT-AG-GGA------------------GTA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-AT-------G-TA-G-G-C-G---------------G--G-AA-G-G-T----------------------AA----G-A-T-G------------G-G-G--G--TG--A-AA-TC--C-A-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-T-G-T-G-A--A-C----T-G--C-C---T--C--------------C--AT-T-A---C---------------T--G-C--CT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-GG------GA-G-A--------------G-G-A-G---AC-T--------------GG--A--ATT-----------C-C-C-A-GT--GT-A-G-GA-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TT-----------------------------G-G---G-A--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----A--G-T-C-T---CTG-----G--CC-T-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GA--T-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CA

Otu0004101 M01867_242_000000000_ATWYL_1_1104_16049_14185 .CGC--G-A-A--TT-TT--TC-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-G-A-A---GC-C-----A--C-GCC-G-T-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---C-----TC-TG-------G-G-T--------T-G-T--A---AA-C-T-GC-------------------------TGTCAGGG-A--A-GGG----G--A-A--A---CG--TCG-G---G-T-G--T---------------------------TA---------------------------AC--------------------------------A-----------------------------G-CAT-C-TG-G-T-T-T-G-A-----C-T---G-C-TT--------T-C-C-A---------A-AG----------G--AA-------------GC-C--CC-G---G-C-AAA-----------------------A--C-A-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AT-GT-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------AT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--C-GA-T-A-T----------------------AA----G-T-C-A------------G-C-G--G--TG--A-AA-TC--C-T-TC-G-G-----------------------------------------------------CC-C-AA-----------C-C-G-G-A-G-A--A-C----T-G--C-C---G--T--------------T--GA-T-A---C---------------T--G-C--AT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-GT------GA-G-G--------------G-G-T-G---GC-T--------------GG--A--ACG-----------A-G-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-C---T-C--G-G-A-AC-A-CC---CAA----G--GC-GAA-G--G-C----A-----G--G-C-C-A---CTG-----G--CA-C-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0004302 M01867_242_000000000_ATWYL_1_1101_22768_8898 .CCT--G-A-C--GG-AG--CG-A-C------GC-C-G-C--GT---------------------------GA-A--T--G-A--A-G-A-A-G-T-C-C---TT-C-----G--G-GAT-G---T-A-----A-A-G--T--TC--------------------------A-A--T--C--G-G-CC---------GG-GG-------A-A-G--------A-A-T--G---GT-T-T-TC--------------------------AT-AC-T-A--A-TAA----T--A-T--G---A-------------------------------------------G--A----------------------------GC--------------------------------A---G--------------------------------------A-G-A-C-G-----G-T---A-C-CC--------G---G-A---------A-T-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AA-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TC-A--C-T--GG-GT-------------------GT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--T-AA-T-A-A----------------------AA----G-T-T-T------------T-G-T--G--TT--T-AA-AT--T-C-AG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-T-G-A-A--C-T----G-G--C-A---T--G--------------A--AG-G-A---C---------------T--G-T--AT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----A-CA------AA-G-A--------------G-G-A-A---AC-T--------------GG--A--ACT-----------C-C-A-G-GT--GA-A-G-CG-GT--G--------A-A-A---TG-T-GT-AG--AT-A-TC-----------------------------T-G---G-G--G-G-A-AC-A-CC----AA-T--G--GC-GAA-G--G-C----A-----G--G-T-T-T---CTG-----G--TT-G-T------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CG

Otu0004476 M01867_242_000000000_ATWYL_1_2108_11218_5233 .CAA--G-A-A--TC-TT--CC-A-C------AA-T-G-G--CC---------------------------GA-A--A--G-G--C-T-G-A-T-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-T-A--T--GA--------------------------T-G--A--A--G-T-CC---------CT-CG-------G-G-A--------T-G-T--A---AA-G-T-AC--------------------------TT-TT-A-T--T-AGG----G--A-T--GA--A----------------------------------------------A----------------------------CA------------------------------------A--------------------------------------T-G-A-C-A-----G-T---A-C-CT--------A-A-T-G---------A-A-----------TA-A--------------GG-G--GC-T---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--AG---C-----------------GG--TA-AT-------AC---GA-AG-GCC------------------CCG-A-G------------C--G--T---T--AT-C-CGG-AT------TA-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--T-TT-G-G-C----------------------GC----A-T-C-T------------T-C-T--G--TT--A-AA-TC--T-T-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-G-G-G-G--G-C----T-G--C-G---G--A--------------A--GA-G-A---T---------------G--G-C--TA--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-GG------GA-G-A--------------G-G-T-A---AG-C--------------GG--A--ATT-----------G-C-T-G-GT--GT-A-G-GG-GT--T--------A-A-A---TC-C-GT-TA--AT-A-TC-----------------------------A-G---C-A--G-G-A-AC-A-CC----AA-A--T--GC-GTA-G--G-C----A-----G--C-T-T-A---CTG-----G--AA-C-G------------------------------------------------------------------------------C-C------A-C-T--GA--CG---CT---------------------------G----A-AA--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--TG

Otu0004649 M01867_242_000000000_ATWYL_1_2105_20794_4955 .GAG--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--C--G-C-GT---------TT-AC-------T-C-G--------T-G-T--A---AA-C-T-CC--------------------------TT-TT-C-T--G-CGG----G--A-A--G---A----------------------------------------------T----------------------------AA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CG--------C-A-G-G---------A-A-----------T--AA-------------GG-G--GC-G---G-C-AAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TG-AT-------AC---GT-AG-GCC------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GC-GT-TC-------G-TA-G-G-C-G---------------G--T-AT-G-G-A----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-GA--C-C-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-C-G-A-G--T-T----T-G--C-A---T--C--------------C--GA-A-A---C---------------T--T-C--CA--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--G-----A-GG------GA-G-A--------------G-G-T-A---AG-C--------------GG--A--ACG-----------G-T-A-T-GA--GT-A-G-GG-GT--G--------C-A-A---TC-C-GT-TG--AT-A-CA-----------------------------T-A---C-C--A-G-A-AC-A-CC----AA-A--A--GC-GAA-G--G-C----A-----G--C-T-T-A---CTG-----G--AA-C-T------------------------------------------------------------------------------C-T------A-T-T--GA--CG---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--AA

Otu0004762 M01867_242_000000000_ATWYL_1_1107_20234_18174 .TTC--G-A-A--TT-TT--TC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--G--GA--------------------------T-G--A--A--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-C-C-CC--------------------------TG-TC-A-G--C-TGG----G--A-A--C---AA-GGTC-T-----C-G--T-G--G-------------------T--G----------------------------AA--------------------------------T---A----G---------------CC---A-TGA-G-AT-T-T-G-A-T-A-----G-T---A-C-CG--------G-C-A-G---------A-G-----------G--AA-------------GC-C--GT-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------TC---AG-AG-ACG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-CC-------G-CA-G-G-T-G---------------G--C-GA-A-G-C----------------------AA----G-T-C-T------------G-A-C--G--TG--A-AA-TC--C-C-GC-G-G-----------------------------------------------------CC-T-AA-----------C-C-G-C-G-G-A--A-C----T-G--C-G---T--C--------------G--GA-T-A---C---------------T--G-C--CT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----C-GG------CA-G-A--------------G-G-A-G---AC-T--------------GG--A--ATT-----------C-A-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-T---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----A--G-T-C-T---CTG-----G--AC-C-G------------------------------------------------------------------------------T-T------C-C-T--GA--CA---C----------------------------T-C--A-GG--G-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CG

Otu0005003 M01867_242_000000000_ATWYL_1_1110_17977_20992 .CGA--G-G-A--TC-AT--TC-G-C------AA-T-G-C--GC---------------------------GA-A--A--G-C--G-T-G-A-C-G-G-T---GC-G-----A--C-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TG-TC-A-T--G-AGG----G--A-A--C---AA-TGTC-C---G-G-G--A-T--G-------------------T--T----------------------------AA--------------------------------T---A--------------------CC---ATTTC-G-GG-G-T-G-A-T-A-----G-T---A-C-CT--------C-A-A-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GCG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-TC-T-G-T----------------------GT----G-T-C-G------------G-A-T--G--TG--A-AA-TC--T-C-GC-A-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-G-G-A--A-T----G-G--C-A---T--T--------------C--GA-A-A---C---------------T--A-T--GG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--A-----C-AG------GA-G-A--------------G-G-A-G---AG-C--------------GG--A--ATT-----------C-T-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-A---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-C-T---CTG-----G--AC-T-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----T-GG--GG-G--AG-C-A-AA--CA

Otu0005465 M01867_242_000000000_ATWYL_1_1104_7951_21001 .GGG--G-A-A--TA-TT--GC-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-G-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--C--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-CC--------------------------TT-TT-G-C--A-GGG----G--A-C--A---A----------------------------------------------T-----------------------------G--------------------------------G------------------------------------------T-G-A-T-G-----G-T---A-C-CC--------T-G-C-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G--C-CG---C-----------------GG--TA-AG-------AC---AG-AG-GTG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------TT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--T-GG-T-G-T----------------------AA----G-T-C-T------------A-G-G--G--TG--G-AA-TG--C-T-AC-A-G-----------------------------------------------------CT-C-AA-----------C-T-G-T-A-G-A--G-C----T-G--C-C---T--T--------------G--GA-A-A---C---------------T--G-C--AT--T-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--C-----G-GT------GA-G-G--------------G-G-A-A---GG-T--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--G-G-A-AG-G-TC----TG-T--G--GC-GAA-G--G-C----G-----A--C-C-T-T---CTG-----G--CA-C-T------------------------------------------------------------------------------T-G------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0005777 M01867_242_000000000_ATWYL_1_1104_17457_23105 .AAA--G-C-C--TG-AC--GG-A-G------CG-A-C-G--CC---------------------------GC-G--T--G-A--A-T-G-A-T-G-A-A---GT-C-----T--T-TCG-G-G-A-T-----G-T-A--A--AG--------------------------T-T--C--A--A-T-CG---------AC-CG-------G-G-G--------A-A-G--A---AT-G-G-TT--------------------------TG-CA-T-A--CTAAT----A--ATAT-G---C-------------------------------------------G--A----------------------------GC--------------------------------A---G--------------------------------------A-G-A-C-G-----G-T---A-C-CC--------G---G-A---------A-T-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AA-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--T-AA-T-A-T----------------------AA----G-T-T-T------------T-G-C--G--TT--T-AA-GG--C-G-GA-G-G-----------------------------------------------------CT-C-AA-----------C-T-T-C-C-G-T--T-A----T-G--C-G---T--G--------------A--AA-G-A---C---------------T--G-T--AT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----A-CG------AA-G-A--------------G-G-A-A---AC-T--------------GG--A--ACT-----------C-C-A-G-GT--GA-A-G-CG-GT--G--------A-A-A---TG-T-GT-AG--AT-A-TC-----------------------------T-G---G-G--G-G-A-AC-A-CC----AA-T--G--GC-GAA-G--G-C----A-----G--G-T-T-T---CTG-----G--TT-G-T------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-A-CG-A--AA-G-C----G-TG--GG-G--AT-C-A-AA--CG

Otu0005979 M01867_242_000000000_ATWYL_1_1107_14065_3091 .CGA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-G-----A--T-GCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-TC--------------------------TG-TC-A-G--A-GGG----G--A-C--G---AA------------------------------------------G--A----------------------------AT--------------------------------G------------------------------------------T-G-A-C-G-----G-T---A-C-CC--------T-C-A-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCG-A-G------------C--G--T---T--AT-T-CGG-AT------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-CC-------G-TA-G-G-G-G---------------G--T-CT-G-C-C----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-TC--C-C-GT-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-C-G-G-A--A-C----T-G--C-A---T--T--------------G--GA-T-A---C---------------T--G-G--CG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--C-----T-GC------GA-G-G--------------G-G-T-G---AG-C--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--A-G-A-AC-A-CC---GAT----G--GC-GAA-G--G-C----G-----G--C-T-C-A---CTG-----G--CG-C-A------------------------------------------------------------------------------G-T------A-C-T--GA--CC---C----------------------------T-G--A-GG--G-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0006223 M01867_242_000000000_ATWYL_1_2106_14254_11953 .CGA--G-A-A--TA-TT--CC-A-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-T-G-G-A---GC-G-----A--T-GCC-G-C-G-T-----G-C-G--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TT-TT-G-T--A-TAG----G--A-A--G---AA---------------------------------------------T----------------------------TT--------------------------------C---G------------------------------------A-T-G-A-C-G-----G-T---A-C-TA--------T-G-C-G---------A-A-----------T--AA-------------GA-G--GT-T---A-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-T--AG---C-----------------GG--TA-AT-------AC---AG-AG-ACC------------------TCA-A-G------------C--G--T---T--AT-C-CGG-AT------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-GG-------G-TA-G-G-T-G---------------G--T-TA-T-A-T----------------------TA----G-T-C-A------------G-A-T--G--TC--A-AA-TC--T-C-GG-A-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-G-A-A--A-C----T-G--C-A---T--T--------------T--GA-A-A---C---------------G--G-T--AT--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-G--G-----T-GT------GA-G-A--------------G-A-C-C---AC-T--------------GG--A--ACT-----------C-A-T-G-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-TG--AT-A-TC-----------------------------A-T---G-A--G-G-A-AC-A-CC----AA-A--G--GC-GAA-G--G-C----A-----T--G-T-G-G---TTG-----G--CG-C-A------------------------------------------------------------------------------C-T------C-T-T--GA--CA---C----------------------------T-G--A-GC--C-G-CG-A--AA-G-C----G-TG--GG-T--AG-C-G-AA--TG

Otu0006685 M01867_242_000000000_ATWYL_1_1101_12140_12975 .GAG--G-A-A--TC-TT--CC-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-G-G-A---GC-G-----A--C-ACC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-G-TC---------TA-AC-------G-A-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-C-T--G-AGG----G--A-G--C---A----------------------------------------------T----------------------------AA---------------------------------------------------------------------------T-G-A-G-A-----G-T---A-C-CT--------C-A-G-G---------A-A-----------T--AA-------------GG-G--AC-G---G-C-TAA-----------------------A--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AT-GTC------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GA-GT-TC-------G-TA-G-G-T-T---------------G--T-TA-G-T-T----------------------AA----G-T-T-G------------G-A-T--A--TG--A-AA-GA--C-C-GG-A-G-----------------------------------------------------CT-C-AA-----------C-T-C-C-G-T-G--T-T----T-G--T-A---T--T--------------C--AA-A-A---C---------------T--G-G--TT--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--T-----A-GG------GA-G-A--------------G-G-T-A---AG-C--------------GG--A--ATT-----------T-T-A-A-GT--GT-A-G-GG-GT--G--------C-A-A---TC-C-GT-AG--AT-A-CT-----------------------------T-A---G-A--A-G-A-AC-A-CC----AA-A--A--GC-GAA-G--G-C----A-----G--C-T-T-A---CTG-----G--AA-C-T------------------------------------------------------------------------------A-T------A-T-T--GA--CG---C----------------------------T-G--A-GG--A-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--AA

Otu0006780 M01867_242_000000000_ATWYL_1_1103_7733_7870 .CGC--G-A-A--TT-TT--TC---TC-----AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-A-G-A-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-G--G--GA--------------------------C-G--A--A--G-G-CC---------CTCTG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TG-TC-A-G--G-GAA----G--G-G--G---AA-ACGC-T---G-C-T--T-A--T----------------------T----------------------------AA--------------------------------T---C--------------------GT---A-GGT-A-GT-T-T-G-ACT-G-----------C-T-TT--------C-C-A-A---------A-G-----------G--AA-------------GC-C--CC-G---G-C-AAA-----------------------A--C-A-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AT-GT-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------AT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-AT-T-G-T----------------------AC----G-T-C-C------------G-G-G--G--TG--A-AA-AC--C-C-AT-G-G-----------------------------------------------------CC-C-AA-----------C-C-A-T-G-G-A--T-C----G-G--C-C---T--T--------------G--GA-T-A---C---------------G--G-C--AG--T-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-GT------GA-G-G--------------G-G-T-G---AT-C--------------GG--A--ACG-----------C-G-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-C---G-C--G-G-A-AG-G-CC----AA-C--G--GC-GAA-G--G-C----A-----G--A-T-C-A---CTG-----G--TA-C-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--AGTG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0006988 M01867_242_000000000_ATWYL_1_2111_22880_8099 .GAG--G-A-A--TC-TT--TC-A-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-G-G-A---GC-G-----A--C-ACC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--A--G-G-AT---------TT-GT-------T-T-T--------C-G-T--A---AA-C-T-CC--------------------------TT-TT-C-T--G-TGG----G--A-C--G---A----------------------------------------------T----------------------------AA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CA--------C-A-G-G---------A-A-----------T--AA-------------GG-G--GC-G---G-C-AAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GCC------------------CCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GT-TT-------G-TA-G-G-C-G---------------G--G-AA-C-G-A----------------------AA----G-T-C-A------------G-G-T--A--TG--A-AA-GC--A-C-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-C-G-T-G----T----C-G--T-G---C--T--------------T--GA-A-A---C---------------T--T-C--GT--T-C-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-A-T--G-----A-GG------GA-G-A--------------G-G-A-A---AG-C--------------GG--A--ATT-----------A-T-G-C-GA--GT-A-A-GG-GT--G--------C-A-A---TC-T-GT-TG--AT-A-CG-----------------------------C-A---T-A--A-G-A-AC-A-CC----AA-A--A--GC-GAA-G--G-C----A-----G--C-T-T-T---CTG-----G--AA-C-T------------------------------------------------------------------------------C-T------A-T-T--GA--CG---C----------------------------T-G--A-GA--A-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--AA

Otu0007024 M01867_242_000000000_ATWYL_1_2112_21178_13806 .TCG--G-A-A--TA-TT--CG-G-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-C-C-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-C-G--G--GA--------------------------T-G--A--A--G-G-CC---------CTCTG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TG-TC-A-C--G-AGT----G--A-G--C---AA-ATTG-G---C-G-T--G-T--T----------------------T----------------------------TA--------------------------------A-----------------------CAA---A-ATA-CGACAT-T-G-A-G-T-----T-A---G-G-CT--------CGA---G---------A-G-----------G--AA-------------GC-C--CC-C---G-C-TAA-----------------------T--C-A-C--G-T---------G-C-CA--G-C---A--G-C--GG---C-----------------GG--TA-AT-------AC---GT-GA-GGG------------------GCA-A-A------------C--G--T---T--GT-T-CGG-TG------TC-A--C-T--GG-GC-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-T-G---------------G--T-TG-C-G-T----------------------AA----G-T-A-A------------G-G-T--G--TG--A-AA-GC--C-C-CC-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-G-G-G-A--A-C----T-G--C-G---C--C--------------T--TA-T-A---C---------------T--G-C--GT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--A-----T-TA------CA-G-G--------------G-G-A-A---GG-G--------------GG--A--ACT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GC-AG--AT-A-TC-----------------------------T-G---G-G--G-G-A-AG-G-CC---GGC----G--GC-GAA-G--G-C----G-----C--C-C-T-T---CTG-----G--GT-A-A------------------------------------------------------------------------------T-A------C-C-T--GA--CA---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0007045 M01867_242_000000000_ATWYL_1_1111_18893_22128 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--GA---------------------------GA-T--A--T-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------A-G--A--A--G-G-CA---------GA-AA-------T-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-G-T--A-CGG----G--A-C--G---AA-TAAA-T---A-T-A--G-T--A-------------------GT-G----------------------------GA--------------------------------A--------A---------------GA---C-TAT-A-TG-A-T-G-A-C-G-----G-T---A-C-TG--------T-A-T-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-TT-------G-TA-G-G-T-G---------------G--T-TA-T-T-T----------------------AA----G-T-G-C------------G-A-G--T--TG--A-AA-GA--C-C-GG-A-G-----------------------------------------------------CT-T-AA-----------C-T-C-T-G-A-G--T-T----T-G--G-C---T--T--------------G--CA-A-A---C---------------T--G-A--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-AG------AA-G-G--------------G-G-T-A---AC-T--------------GG--A--ATT-----------C-C-G-C-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TG-----------------------------C-G---G-A--A-G-A-AT-A-CT----TG-T--G--GC-GAA-G--G-C----G-----A--G-T-T-A---CTG-----G--TC-T-G------------------------------------------------------------------------------T-T------A-C-T--GA--CA---C----------------------------T-G--A-AA--A-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0007162 M01867_242_000000000_ATWYL_1_1112_17625_14607 .AAA--G-C-C--TG-AC--GG-A-G------CG-A-C-G--CC---------------------------GC-G--T--G-A--A-T-G-A-A-G-A-A--GTCTT-----T--C-GGG-A-T-G-T-----A-A-A--G--TT--------------------------C-G--AT-C--G-G-CC---------GG-GG-------A-A-G--------A-A-T--G---GT-T-C-TC--------------------------AT-GG-T-A--A-TAT----A--A-T--G---A-------------------------------------------G--A----------------------------GC--------------------------------A---G--------------------------------------G-G-A-C-G-----G-T---A-C-CC--------G---G-T---------A-C-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AA-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--T-AA-T-G-T----------------------AA----G-T-T-T------------C-A-C--G--TT--T-AA-TG--T-A-TT-G-G-----------------------------------------------------CT-C-AA-----------C-C-A-G-T-A-T--T-T----G-G--C-G---T--G--------------A--AA-G-A---C---------------T--G-C--AT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----A-TA------GA-G-A--------------G-G-A-A---AC-T--------------GG--A--ACT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-T-GT-AG--AT-A-TC-----------------------------T-G---G-G--G-G-A-AC-A-CC----GA-T--G--GC-GAA-G--G-C----A-----G--G-T-T-T---CTG-----G--CT-A-T------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--CG

Otu0007202 M01867_242_000000000_ATWYL_1_1108_4895_16639 .CGA--G-G-A--TC-TT--GG-A-C------AA-T-G-C--GC---------------------------GA-A--A--G-C--G-T-G-A-T-C-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TG-TC-G-T--G-AGG----G--A-G--C---AA-TAGC-G---T-G-G--G-G--G-------------------C--T----------------------------AA--------------------------------C---A----C---------------CC---T-CTG-C-GC-A-T-G-A-T-A-----G-T---A-C-CT--------C-A-G-G---------A-G-----------G--AA-------------GA-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTG------------------TCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-TT-C-G-T----------------------GT----G-T-C-G------------G-A-T--G--TG--A-AA-TC--C-T-GC-C-G-----------------------------------------------------CT-T-AA-----------C-G-G-C-A-G-A--A-C----G-G--C-A---T--C--------------C--GA-A-A---C---------------T--A-C--GG--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--T-----C-GG------GA-G-A--------------G-G-A-A---GG-C--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-G---G-A--G-G-A-AC-A-CC----CG-T--G--GC-GAA-G--G-C----G-----G--C-C-T-T---CTG-----G--AC-C-G------------------------------------------------------------------------------A-C------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AG-G-C----T-GG--GG-G--AG-C-A-AA--CA

Otu0007891 M01867_242_000000000_ATWYL_1_2102_9749_8306 .TCG--G-A-A--TA-TT--CG-G-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-C-C-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-C-G--G--GA--------------------------T-G--A--A--G-G-CC---------CTCTG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TG-TC-A-C--G-AGT----G--A-G--C---AAATTGTCT---T-GTT--T-T-------------------------A----------------------------AT----------------------------------------------------------A---CAATAGGACAT--T-G-AGT-T-----A-A---G-C-TC--------G-A---G---------A-G-----------G--AA-------------GC-C--CC-C---G-C-TAA-----------------------T--C-A-C--G-T---------G-C-CA--G-C---A--G-C--GG---C-----------------GG--TA-AT-------AC---GT-GA-GGG------------------GCT-A-A------------C--G--T---T--GT-T-CGG-TG------TC-A--C-T--GG-GC-------------------TT--A-----AA-GG-GC-GC-------G-TA-G-G-T-G---------------G--C-TA-C-G-T----------------------AA----G-T-A-A------------G-G-T--G--TG--A-AA-GC--C-C-CC-G-G-----------------------------------------------------CT-T-AC-----------C-C-G-G-G-G-A--A-T----G-G--C-G---C--C--------------T--TA-T-A---C---------------T--G-C--GT--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--A-----T-CA------CA-G-G--------------G-G-A-A---GG-G--------------GG--A--ACT-----------C-C-A-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GC-AG--AT-A-TC-----------------------------T-G---G-G--G-G-A-AG-G-CC----GG-C--G--GC-GAA-G--G-C----G-----C--C-C-T-T---CTG-----G--GT-G-A------------------------------------------------------------------------------T-T------C-C-T--GA--CA---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0008028 M01867_242_000000000_ATWYL_1_1111_17850_10816 .CGA--G-G-A--TC-TT--GG-A-C------AA-T-G-T--GC---------------------------GA-A--A--G-C--A-T-G-A-T-C-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--C--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TG-TC-G-T--G-AGG----G--A-G--C---AA-TGAC-G---C-G-G--G-C--G-------------------A--T----------------------------AA--------------------------------C---A--------------------CC---GTTCG-C-GT-G-T-G-A-T-A-----G-T---A-C-CT--------C-A-G-G---------A-G-----------G--AA-------------GA-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTG------------------TCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-AT-C-G-T----------------------GT----G-T-C-G------------G-T-C--G--TG--A-AA-TC--C-T-GC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-C-A-G-A--A-C----G-G--C-G---T--C--------------C--GA-A-A---C---------------T--A-C--GA--G-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-GA------CA-G-A--------------G-G-A-A---GT-C--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--A-C-T-T---CTG-----G--GT-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AG-G-C----C-GG--GG-G--AG-C-A-AA--CA

Otu0008201 M01867_242_000000000_ATWYL_1_1105_6399_14982 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--GA---------------------------GT-A--A--T-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------T-G--A--A--G-G-CA---------GA-AA-------T-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-T-A--A-CGG----G--A-A--G---AA-TAAG-T---G-TAG--T-G--A-------------------G--A----------------------------GA--------------------------------A--------A---------------CG---C-TAT-A-TG-A-T-G-A-C-G-----G-T---A-C-CG--------T-T-A-G---------A-A-----------T--AA-------------GT-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------ACA-A-G------------C--G--T---T--GT-T-CGG-AT------TC-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AT-------G-TA-G-G-C-G---------------G--T-AT-A-A-T----------------------AA----G-T-G-T------------G-A-A--T--TG--A-AA-GA--C-C-GG-G-G-----------------------------------------------------CT-T-AA-----------C-C-C-C-G-A-G--T-T----T-G--G-T---T--T--------------G--CA-A-A---C---------------T--G-T--TA--T-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-GG------GA-G-A--------------G-G-A-G---AC-T--------------GG--A--ATT-----------C-C-G-T-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TG-----------------------------C-G---G-A--A-G-A-AT-A-CT----TA-T--G--GC-GAA-G--G-C----A-----A--G-T-C-T---CTG-----G--CC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-AA--T-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0008653 M01867_242_000000000_ATWYL_1_1109_18266_18624 .TAA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-G-A---GC-G-----A--T-GCC-G-C-G-T-----G-A-T--C--GA--------------------------T-G--A--A--G-G-TC---------GA-GA-------G-A-T--------T-G-T--A---AA-G-A-TC--------------------------TT-TT-C-C--A-ACT----G--A-G--G---AA-TAAG-C---A-T-G--G-G--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GC---C-CGT-G-TG-A-T-G-A-C-G-----T-T---A-A-GT--------T-G-G-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AC-------AC---GT-AA-GGG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AC-------G-CA-G-G-C-G---------------G--T-TA-T-G-C----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-TC--T-C-CG-G-G-----------------------------------------------------CT-T-AA-----------C-T-C-G-G-A-A--T-C----C-G--C-G---T--T--------------G--GA-A-A---C---------------T--G-C--AT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----T-TA------GA-G-G--------------G-G-G-A---GT-C--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-TG--AT-A-TT-----------------------------A-G---G-A--A-G-A-AC-A-CC---GGT----G--GC-GAA-G--G-C----G-----G--A-C-T-C---CTG-----G--CT-G-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--AA-G-C----A-TA--GG-G--AG-C-A-AA--CA

Otu0008874 M01867_242_000000000_ATWYL_1_2109_5719_9979 .CGC--G-A-A--TT-TT--TC-A-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-T-G-A-A---GC-C-----A--C-GCC-G-T-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---C-----TC-TG-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TG-TC-A-G--G-GAA----G--G-G--G---AA--ACG-C---C-G-G--G---------------------------T---------------------------GTT--------------------------------A-----------------------------A-GAG-CATC-T-G-GTT-T-GA----C-T---G-C-TT--------T-C-C-A---------A-AG----------G--AA-------------GC-C--CC-G---G-C-AAA-----------------------A--C-A-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AT-GT-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------AT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--C-GA-T-A-T----------------------AA----G-T-C-A------------G-C-G--G--TG--A-AA-TC--C-T-TC-G-G-----------------------------------------------------CC-C-AA-----------C-C-G-G-A-G-A--A-C----T-G--C-C---G--T--------------T--GA-T-A---C---------------T--G-C--AT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-GT------GA-G-G--------------G-G-T-G---GC-T--------------GG--A--ACG-----------A-G-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-C---T-C--G-G-A-AC-A-CC---CAA----G--GC-GAA-G--G-C----A-----G--G-C-C-A---CTG-----G--CA-C-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0008978 M01867_242_000000000_ATWYL_1_2108_5883_15831 .CAA--G-A-A--TT-TT--CC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--G--GA--------------------------A-G--A--A--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-G-T--A-AGG----G--A-A--G---AA-TAAG-G---T-T-G--G-T--A-------------------G--G----------------------------AA--------------------------------A--------T---------------GA---C-CGA-C-TG-A-T-G-A-C-G-----G-T---A-C-CT--------T-A-C-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AA-GGG------------------GCG-A-G------------C--G--T---T--GC-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GT-------G-TA-G-G-C-G---------------G--T-TA-T-G-C----------------------AA----G-T-C-G------------A-A-T--G--TA--A-AA-AA--C-A-GT-A-G-----------------------------------------------------CT-T-AA-----------C-T-A-C-T-G-G--T-C----T-G--C-G---T--T--------------C--GA-A-A---C---------------T--G-C--AT--A-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--C-----T-GG------TG-G-A--------------G-G-G-T---GA-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--A-G-A-AC-A-CC----GA-A--G--GC-TAA-A--G-C----A-----G--T-C-A-T---CCA-----T--GC-C-A------------------------------------------------------------------------------A-G------A-T-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----A-TG--GG-T--AT-C-A-AA--CA

Otu0009272 M01867_242_000000000_ATWYL_1_2114_15209_22237 .CGA--G-G-A--TC-AT--TC-G-C------AA-T-G-C--GC---------------------------GA-A--A--G-C--G-T-G-A-C-G-G-T---GC-G-----A--C-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-C-T-GC--------------------------TG-TC-A-T--G-AGG----G--A-A--C---AA-TGTC-C---G-G-G--A-T--G-------------------T--A----------------------------AA--------------------------------T---A----T---------------CA---TCTCG---GG-G-T-G-A-T-A-----G-T---A-C-CT--------C-A-A-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GCG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-TC-T-G-T----------------------GT----G-T-C-G------------G-A-C--G--TG--A-AA-TC--T-C-AC-A-G-----------------------------------------------------CT-C-AA-----------C-T-G-T-G-G-A--A-T----T-G--C-A---T--T--------------C--GA-A-A---C---------------T--A-C--AG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--A-----C-AG------GA-G-A--------------G-G-A-G---AG-C--------------GG--A--ATT-----------C-T-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-A---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-C-T---CTG-----G--AC-T-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----T-GG--GG-G--AG-C-A-AA--CA

Otu0009332 M01867_242_000000000_ATWYL_1_2111_5860_13479 .AAG--C-C-T--GA-CG--GA-G-C------AA-T---G--CC---------------------------GC-G--T--G-C--A-C-G-A-C-G-A-A---GG-C-----C--T-TCG-G-G-T-T-----G-T-A--A--AG--------------------------T-G--C--T--G-T-CA---------GA-AG-------G-G-A--------A-G-A--AG--GT-T-T-TC--------------------------CA-GT-C-G--A-ACA----G--ATT--G---G-------------------------------------------A--A----------------------------GG--------------------------------C---T--------------------------------------T-G-A-C-G-----G-T---A-C-CT--------T-C-A-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AA-GTG------------------GCG-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GG-GT-GT-------G-TA-G-G-C-G---------------G--C-TT-A-C-T----------------------AA----G-T-C-C------------T-G-T--G--TT--A-AA-TC--C-C-AC-G-G-----------------------------------------------------CT-T-AA-----------C-T-G-T-G-G-G--C-T----T-G--C-G---C--G--------------G--GA-A-A---C---------------T--G-G--TA--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----G-AG------GA-G-A--------------G-G-T-A---AC-T--------------GG--A--ATT-----------C-C-T-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-T-GT-AG--AT-A-TT-----------------------------A-G---G-A--A-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--G-T-T-A---CTG-----G--CC-T-C------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-G--A-GA--C-A-CG-A--AA-G-C----C-GG--GG-G--AG-C-A-AA--CA

Otu0009536 M01867_242_000000000_ATWYL_1_1114_8559_22018 .CGC--G-A-A--TT-TT--TC---TC-----AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-A-G-A-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-G--G--GA--------------------------C-G--A--A--G-G-CC---------CTCTG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TG-TC-A-G--G-GAA----G--G-G--G---AA-ACGT-G---G-C-G--C-A--T----------------------T----------------------------AA--------------------------------T---C--------------------G----T-GCG-T-CATT-T-G-ACT-G-----------C-T-TT--------C-C-A-A---------A-G-----------G--AA-------------GC-C--CC-G---G-C-AAA-----------------------A--C-A-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AT-GT-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--C-T--GG-GC-------------------AT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-AT-T-G-T----------------------AC----G-T-C-C------------G-G-G--G--TG--A-AA-AC--C-C-AT-G-G-----------------------------------------------------CC-C-AA-----------C-C-A-T-G-G-A--T-C----G-G--C-C---T--T--------------G--GA-T-A---C---------------G--G-C--AG--T-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-GT------GA-G-G--------------G-G-T-G---AT-C--------------GG--A--ACG-----------C-G-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-C---G-C--G-G-A-AG-G-CC----AA-C--G--GC-GAA-G--G-C----A-----G--A-T-C-A---CTG-----G--CA-C-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--AGTG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0009558 M01867_242_000000000_ATWYL_1_1111_2481_17703 .AAC--G-A-A--TC-TT--CC-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCT-G-C-G-T-----G-T-G--G--GA--------------------------T-G--A--A--G-C-CC---------TT-CG-------G-G-G--------T-G-T--A---AA-C-C-AC--------------------------TG-TC-G-G--G-ATT----C--A-G--C---A-------------------------------------------A--C----------------------------AA--------------------------------A---A--------------------------------------T-G-A-G-C-----A-A---A-T-CC--------A-A-A-G---------G----------------AA-------------GA-G--GC-G---A-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-T--CG---C-----------------GG--TA-AT-------AC---AG-AG-GCC------------------TCG-A-G------------C--G--T---T--GC-G-CGG-AG------TC-A--C-T--GG-GC-------------------TT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--C-TG-T-G-T----------------------AA----G-T-G-C------------T-T-T--G--TG--A-AA-TC--C-C-AC-G-G-----------------------------------------------------CT-C-AA-----------C-C-G-T-G-G-A--A-T----T-G--C-T---T--G--------------G--CA-G-A---C---------------T--G-C--AT--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-G--T-----T-CG------CA-G-A--------------G-G-T-T---GT-C--------------GG--A--ACT-----------C-T-A-G-GT--GG-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------T-A---G-A--G-G-A-AC-G-CC----GA-A--G--GT-GAA-G--A-C----A-----G--G-C-A-A---CTG-----G--GC-G-A------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CG

Otu0009937 M01867_242_000000000_ATWYL_1_1114_24284_17115 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--GA---------------------------GA-T--A--T-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------T-G--A--A--G-G-CG---------GA-AA-------C-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-A-T--A-CGG----G--A-A--G---AATAAAG-A---C-A-C--T-G--A-------------------G--A----------------------------GA--------------------------------A--------A---------------CG---G-TGT-A-AG-A-T-G-A-C-T-----G-T---A-C-CG--------T-A-T-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-TT-------G-TA-G-G-T-G---------------G--C-GA-T-A-T----------------------AA----G-T-G-T------------G-A-G--T--TG--A-AA-GA--C-C-GA-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-C-G-A-G--T-T----T-G--G-C---T--T--------------G--CA-A-A---C---------------T--G-T--AT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-AG------GA-G-G--------------G-G-T-G---AC-T--------------GG--A--ATT-----------C-C-A-T-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TA-----------------------------T-G---G-A--A-G-A-AT-A-CT----TG-T--G--GC-GAA-G--G-C----G-----A--G-T-C-A---CTG-----G--CC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-AA--A-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0010349 M01867_242_000000000_ATWYL_1_1107_11781_18752 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--GA---------------------------GA-T--A--T-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-A--C--GA--------------------------A-G--A--A--G-G-CA---------GA-AA-------T-G-T--------T-G-T--A---AA-G-T-TC--------------------------TT-TT-A-T--A-CGG----G--A-C--G---AA-TAAG-T---A-T-AT-T-G--A-------------------G--A----------------------------GA--------------------------------A--------A---------------CC---T-TAT-A-CG-A-T-G-A-C-G-----G-T---A-C-CG--------T-A-T-G---------A-A-----------T--AA-------------GT-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------ACA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GC-AT-------G-TA-G-G-C-G---------------G--C-GA-G-A-T----------------------AA----G-T-G-T------------G-A-A--T--TG--A-AA-GA--C-C-GA-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-C-G-C-G--T-T----T-G--G-T---T--T--------------G--CA-A-A---C---------------T--G-T--CT--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--A-----T-GG------AA-G-G--------------G-G-A-G---AC-T--------------GG--A--ATT-----------C-C-G-C-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TG-----------------------------C-G---G-A--A-G-A-AT-A-CT---TGT----G--GC-GAA-G--G-C----G-----A--G-T-C-T---CTA-----G--TC-T-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-AA--T-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0010420 M01867_242_000000000_ATWYL_1_2101_19643_5469 .TAA--G-A-A--TC-TT--AG-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-C-T-A---GC-G-----A--C-GCC-G-A-G-T-----G-G-A--T--GA--------------------------T-G--A--A--G-G-CCT--------TT-TT-------G-G-T--------T-G-T--A---AA-A-T-CC--------------------------TT-TT-A-C--A-TGG----G--A-A--G---AA-T-GT-C---T-G-T--G----A----------------------A----------------------------AA--------------------------------A--------T----------------C-----A---G--G-T-T-G-A-C-T-----G-T---A-C-CA--------T-G-G-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--CA-AT-------AC---GT-GA-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-TT-G-G-T----------------------AA----G-T-T-G------------G-A-T--G--TG--C-AA-TC--T-C-CG-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-G-G-A-A--A-C----T-G--C-G---T--C--------------C--AA-A-A---C---------------T--A-C--TT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-AG------TA-G-A--------------G-A-G-A---AG-C--------------GG--A--ATT-----------C-C-A-G-GT--GT-A-G-GA-GT--G--------A-A-A---TC-T-TT-AG--AT-A-TC-----------------------------T-G---G-A--A-G-A-AC-A-CC----GG-C--G--GC-GAA-G--G-C----G-----G--C-T-T-C---TTA-----G--AC-T-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-C--A-GG--C-A-CG-A--AA-G-C----C-AG--GG-G--AG-C-A-AA--CG

Otu0010809 M01867_242_000000000_ATWYL_1_2102_23761_14896 .GGG--G-A-A--TT-TT--GC-A-C------AA-T-G-G-GCC---------------------------AA-A--A--G-C--C-T-G-A-T-G-C-A---GC-G-----A--T-GCC-G-C-G-T-----G-G-A--T--GA--------------------------A-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-T-CC--------------------------TT-TC---G----A------C--A-G--G---AA--A---------G------A--A---------------------------------------------------GA------------------------------------A-----------------------------A-A------T-G-A-C-G-----G-T---A-C-CT--------G-G-A-G---------A-A-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------T--C-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---GT-GA-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GA-GC-GC-------G-TA-G-G-C-G---------------A--C-TC-T-G-C----------------------AA----G-T-C-A------------G-A-G--G--TG--A-AA-AG--T-C-AG-G-G-----------------------------------------------------CT-T-AA-----------C-C-T-T-G-G-C--C-T----T-G--C-C---T--T--------------T--GA-A-A---C---------------T--G-C--AG--A-G-A-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-AG------GA-T-A--------------G-G-A-G---GG-T--------------GG--A--ACT-----------A-C-A-A-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TT-----------------------------T-G---T-A--G-G-A-AT-G-CC----GA-T--G--AC-GAA-G--G-T----A-----A--C-T-C-T---CTG-----G--AC-T-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--GA-G-C----T-AG--GG-G--AG-C-A-AA--CA

Otu0010835 M01867_242_000000000_ATWYL_1_2101_9539_19128 .CGC--G-A-A--TT-TT--TC---AC-----AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-T-G-A-A---GC-C-----A--C-GCC-G-C-G-T-----G-C-G--G--GA--------------------------T-G--A--A--G-G-CC---------CTCTG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TG-TC-A-G--G-GGA----G--G-G--G---AA-ACGC-A---T-G-G--T-G--T----------------------T----------------------------AA--------------------------------C---A--------------------GC---G-CCA-T-GT-T-T-G-ACT-G-----------C-T-CT--------C-C-A-A---------A-G-----------G--AA-------------GC-C--CC-G---G-C-AAA-----------------------A--C-A-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AT-GT-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--C-T--GG-GC-------------------AT--A-----AA-GG-GT-GC-------G-TA-G-G-T-G---------------G--C-AT-G-T-T----------------------AA----G-T-C-A------------C-G-G--G--TG--A-AA-GT--C-T-GG-G-G-----------------------------------------------------CC-C-AA-----------C-C-T-T-A-G-A--A-T----C-G--C-C---T--G--------------T--GA-T-A---C---------------T--G-G--CG--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-GT------AA-G-G--------------G-G-T-A---AC-C--------------GG--A--ACG-----------A-G-C-A-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TT-----------------------------G-C---T-C--G-G-A-AG-G-CC----AG-A--G--GC-GAA-G--G-C----G-----G--G-T-T-A---CTG-----G--TG-C-G------------------------------------------------------------------------------C-A------A-C-T--GA--CA---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0010858 M01867_242_000000000_ATWYL_1_2104_21100_12395 .GGG--G-A-A--TC-TT--GC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-C-A---GC-C-----A--C-GCC-G-C-G-T-----G-G-G--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------C-G-T--A---AA-G-C-CC--------------------------TG-TC-G-G--G-AGG----G--A-A--GA--A-------------------------------------------A-GCCTGCGCAA--------------------GCAGGAT----------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CT--------C-G-C-A---------A-G-----------A--AA-------------GC-G--CC-G---G-C-TAA-----------------------C--T-C-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GG-AG-GGC------------------GCA-A-G------------C--G--T---T--GC-T-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GC-GC-AC-------G-CA-G-G-C-G---------------G--T-CG-A-T-T----------------------AA----G-T-C-G------------G-A-T--G--TG--A-AA-TC--C-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-T-G-G-A--A-A----T-G--C-A---T--T--------------C--GA-A-A---C---------------T--G-G--TT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--A-----C-GA------GA-G-A--------------G-G-G-A---AG-C--------------GG--A--ATT-----------C-C-T-G-GT--GT-A-G-AG-GT--G--------A-A-A---TT-C-GT-AG--AT-A-TC-----------------------------A-G---G-A--G-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--C-T-T-C---CTG-----G--CT-C-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-C--A-TG--T-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0011837 M01867_242_000000000_ATWYL_1_2107_22483_18238 .CGA--G-G-A--TA-AT--TC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-A-T---GC-G-----A--C-GCC-G-C-A-T-----G-A-A--C--GA--------------------------T-G--A--A--G-G-CC---------TT-TG-------G-G-T--------T-G-T--A---AC-G-T-TC--------------------------TG-TT-G-C--C-CTA----G--A-A--C---AA--GAG-T---A-C-T--C-G--A-------------------C--G----------------------------AA--------------------------------T---A----G---------------TT---G-GGT-A-CA-A-T-G-A-G-A-----G-T---A-T-AG--------G-G-A-G---------A-G-----------G--AA-------------GC-A--CC-A---G-C-TAA-----------------------T--T-C-C--G-T---------G-C-CA--G-C---A--G-C--TG---C-----------------GG--TA-AT-------AC---GG-AA-GGT------------------GCA-A-G------------C--G--T---T--AG-T-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-T---------------G--G-AA-T-A-T----------------------AA----G-T-T-G------------G-T-T--G--TG--A-AA-TA--C-C-GG-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-C-G-G-A--A-C----A-G--C-A---A--A--------------C--AA-T-A---C---------------T--G-T--AT--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAA-G-A-G-G--A-----G-TG------GC-G-G--------------A-G-A-G---AA-T--------------GG--A--ATT-----------C-C-A-T-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TA-----------------------------T-G---G-A--A-G-A-AC-A-CC----TG-T--G--GC-GAA-A--G-C----G-----G--T-T-C-T---CTA-----G--CT-T-C------------------------------------------------------------------------------C-T------C-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----A-AG--GG-G--AG-C-A-AA--CA

Otu0011908 M01867_242_000000000_ATWYL_1_2105_7324_15109 .GAG--G-A-A--TC-TT--CC-A-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-G-G-A---GC-G-----A--C-ACC-G-C-G-T-----G-A-A--G--GA--------------------------T-G--A--A--G-C-CT---------TT-AT-------T-G-G--------T-G-T--A---AA-C-T-TC--------------------------TT-TT-C-T--G-TGG----G--A-A--G---A----------------------------------------------T----------------------------AA---------------------------------------------------------------------------T-G-A-C-G-----G-T---A-C-CA--------C-A-G-G---------A-A-----------T--AA-------------GG-G--GC-G---G-C-AAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GCC------------------CCG-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GT-------------------GT--A-----AA-GC-GT-GC-------G-TA-G-G-T-G---------------G--G-AT-G-G-A----------------------AA----G-T-C-A------------G-A-T--G--TG--A-AA-GA--C-T-GG-G-G-----------------------------------------------------CT-T-AA-----------C-C-C-C-A-T-G--T-T----T-G--C-A---T--C--------------C--GA-A-A---C---------------T--C-C--CA--T-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T--T-----A-GG------GA-G-A--------------G-G-T-A---AG-C--------------AG--A--ATC-----------G-T-A-T-GA--GT-A-G-GG-GT--G--------C-A-A---TC-C-GT-TG--AT-A-CA-----------------------------T-A---C-G--A-G-A-AT-A-CC----AA-A--A--GC-GAA-G--G-C----A-----G--C-T-T-A---CTG-----G--AA-C-T------------------------------------------------------------------------------A-T------A-T-T--GA--CA---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-G-AA--AA

Otu0012712 M01867_242_000000000_ATWYL_1_1101_16958_4443 .GGG--G-A-A--TT-TT--GC-A-C------AA-T-G-G--GCT--------------------------AA-A--A--G-C--C-T-G-A-T-G-C-A---GC-G-----A--T-GCC-G-C-G-T-----G-G-A--T--GA--------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-G-T-CC--------------------------TT-TC-G-A--C-GGG----G--A-A--G---A-------------------------------------------A--G----------------------------AA--------------------------------A---A--------------------------------------T-G-A-C-G-----G-T---A-C-CT--------G-G-A-G---------A-A-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------T--C-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AG-------AC---GT-GA-GTG------------------GCA-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--C-T--GG-GC-------------------GT--A-----AA-GA-GC-GC-------G-TA-G-G-C-G---------------A--C-TT-T-G-C----------------------AA----G-T-C-A------------G-A-G--G--TG--A-AA-AG--T-C-AG-G-G-----------------------------------------------------CT-T-AA-----------C-C-T-T-G-G-C--C-T----T-G--C-C---T--T--------------T--GA-A-A---C---------------T--G-C--AG--A-G-A-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-AG------GA-T-A--------------G-G-A-G---GG-T--------------GG--A--ACT-----------A-C-A-A-GT--GT-A-G-CG-GT--G--------G-A-A---TG-C-GT-AG--AT-A-TT-----------------------------T-G---T-A--G-G-A-AT-G-CC----GA-T--G--AC-GAA-G--G-T----A-----A--C-T-C-T---CTG-----G--AC-T-G------------------------------------------------------------------------------A-T------A-C-T--GA--CG---C----------------------------T-G--A-GG--T-G-CG-A--GA-G-C----T-AG--GG-G--AG-C-A-AA--CA

Otu0013955 M01867_242_000000000_ATWYL_1_1114_5898_18696 .CGA--G-A-A--TC-TT--CC-G-C------AA-T-G-G--AC---------------------------GC-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-T--T--GA--------------------------T-G--A--A--G-T-CC---------TT-CG-------G-G-A--------C-G-T--A---AA-G-A-TC--------------------------TT-TT-C-T--C-AAG----G--A-G--G---AA------------------------------------------G--T----------------------------TT------------------------------------A------------------------------------T-T-G-A-C-G-----T-T---A-C-TT--------G-A-G-G---------A-A-----------T--AA-------------GA-G--GT-T---G-C-TAA-----------------------A--C-T-C--G-T---------G-C-CA--G-C---A--G-C--AG---C-----------------GG--TA-AT-------AC---GA-GT-GCC------------------TCG-A-G------------C--G--T---T--AT-C-CGG-AA------TT-A--T-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-TT-T-G-T----------------------TA----G-T-C-T------------T-T-T--G--TT--A-AA-GC--T-C-CG-G-G-----------------------------------------------------CA-C-AA-----------C-T-C-G-G-A-A--A-A----A-G--C-A---A--A--------------A--GA-A-A---C---------------G--G-C--A---A-GTC-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-A--A-----T-GT------TA-G-G--------------G-G-T-A---AG-C--------------GG--A--ACT-----------C-T-A-T-GT--GT-A-G-GG-GT--G--------A-A-A---TC-C-GT-TG--AT-A-TA-----------------------------T-A---G-G--G-G-A-AC-A-CC----AA-A--A--GC-GAA-G--G-C----A-----G--C-T-T-A---CTG-----G--AA-C-A------------------------------------------------------------------------------T-T------T-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-T----G-TG--GG-T--AG-C-G-AA--TG

Otu0014585 M01867_242_000000000_ATWYL_1_1107_14059_6729 .CGC--G-A-A--TT-TT--TC---TC-----AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-A-G-A-A---GC-C-----A--T-GCC-G-C-G-T-----G-C-G--G--GA--------------------------C-G--A--A--G-G-CC---------CTCTG-------G-G-T--------T-G-T--A---AA-C-C-GC--------------------------TG-TC-A-G--G-GAA----G--G-G--G---AA-ACGC-T---G-C-A--C-A--T----------------------G----------------------------AA--------------------------------C---A-------------------GTG---T-GCA-G-TT-T-G-A-C-T-G-----------C-T-TT--------C-C-A-A---------A-G-----------G--AA-------------GC-C--CC-G---G-C-AAA-----------------------A--C-A-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AT-GT-GGG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--C-T--GG-GC-------------------AT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-AT-T-G-T----------------------AC----G-T-C-C------------G-G-G--G--TG--A-AA-AC--C-C-AT-G-G-----------------------------------------------------CC-C-AA-----------C-C-A-T-G-G-A--T-C----G-G--C-C---T--T--------------G--GA-T-A---C---------------G--G-C--AG--T-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--G-----C-GT------GA-G-G--------------G-G-T-G---AT-C--------------GG--A--ACG-----------C-G-T-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-C---G-C--G-G-A-AG-G-CC----AA-C--G--GC-GAA-G--G-C----A-----G--A-T-C-A---CTG-----G--CA-C-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--AGTG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0014697 M01867_242_000000000_ATWYL_1_1111_6822_16178 .CGA--G-G-A--TC-TT--GG-A-C------AA-T-G-T--GC---------------------------GA-A--A--G-C--A-T-G-A-T-C-C-A---GC-G-----A--C-GCC-G-C-G-T-----G-C-A--G--GA--------------------------T-G--A--C--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-GC--------------------------TG-TC-G-T--G-AGG----G--A-G--C---AA-TGAC-G---C-G-G--G-C--G-------------------A--T----------------------------AA--------------------------------T---A--------------------CC---TCTCG-C-GT-G-T-G-A-T-A-----G-T---A-C-CT--------C-A-G-G---------A-G-----------G--AA-------------GA-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTG------------------TCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--A-TT-C-G-T----------------------GT----G-T-C-G------------G-A-C--G--TG--A-AA-TC--C-T-GC-G-G-----------------------------------------------------CT-T-AA-----------C-C-G-C-A-G-A--A-C----G-G--C-G---T--C--------------C--GA-A-A---C---------------T--A-C--GG--A-T-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--T-----C-GG------GA-G-A--------------G-G-A-A---GT-C--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----AG-T--G--GC-GAA-G--G-C----G-----G--A-C-T-T---CTG-----G--AC-C-G------------------------------------------------------------------------------A-C------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AG-G-C----C-GG--GG-G--AG-C-A-AA--CA

Otu0016935 M01867_242_000000000_ATWYL_1_1104_14429_22167 .TAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--GA---------------------------GC-A--A--T-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-A--C--GA--------------------------A-G--A--A--G-G-CA---------GA-AA-------T-G-T--------T-G-T--A---AA-G-T-CC--------------------------TT-TT-A-T--A-TGG----G--A-A--G---AA-TAAGGT---T-A-G--A-A--A-------------------T--G----------------------------GA--------------------------------A--------T---------------AA---T-TAA-T-CG-A-T-G-A-C-G-----G-T---A-C-CA--------T-A-T-G---------A-A-----------T--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GTG------------------GCA-A-G------------C--G--T---T--GT-T-CGG-AA------TT-A--C-T--GG-GC-------------------AT--A-----AA-GG-GC-GT-------G-TA-G-G-C-G---------------G--T-TA-T-A-T----------------------AA----G-T-G-T------------G-A-G--T--TG--A-AA-GA--C-C-AA-A-G-----------------------------------------------------CT-T-AA-----------C-T-T-T-G-A-G--T-T----T-G--G-C---T--T--------------G--CA-A-A---C---------------T--G-T--AT--A-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--G-----C-AG------AA-G-G--------------G-G-G-A---AC-T--------------GG--A--ATT-----------C-C-G-C-GT--GT-A-G-GG-GT--G--------A-A-A---TC-T-GT-AG--AT-A-TG-----------------------------C-G---G-A--A-G-A-AT-A-CT----TG-T--G--GC-GAA-G--G-C----G-----A--G-T-T-C---CTG-----G--TC-T-G------------------------------------------------------------------------------T-A------A-C-T--GA--CG---C----------------------------T-G--A-AA--C-G-CG-A--AA-G-T----G-TG--GG-G--AG-C-A-AA--CA

Otu0017216 M01867_242_000000000_ATWYL_1_1102_26243_15028 .GAG--G-A-A--TT-TT--CC-G-C------AA-T-G-G--GC---------------------------GC-A--A--G-C--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--G--GA--------------------------T-G--A--A--G-G-CT---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-TC--------------------------TT-TT-G-C--G-GGG----G--A-A--A---AA-GGTG-T---T-T-G--T-G--C-------------------T--C----------------------------AT--------------------------------A------------------------CC---A-CAG-GCAC-T-T-G-A-T-G-----G-T---A-C-TC--------C-G-C-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------C--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGG------------------GCG-A-G------------C--G--T---T--GT-C-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GT-------G-TA-G-G-C-G---------------G--T-TG-G-A-T----------------------AA----G-T-C-T------------G-A-T--G--TG--A-AA-TC--T-C-AT-G-G-----------------------------------------------------CT-C-AA-----------C-C-A-T-G-A-G--A-C----T-G--C-G---T--T--------------G--GA-A-A---C---------------T--G-T--CT--G-A-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--T-----C-GG------CA-G-G--------------G-G-T-T---AC-T--------------GG--A--ATC-----------C-C-C-G-GT--GT-A-A-CG-GT--G--------A-A-A---TG-T-GT-AG--AT-A-TC-----------------------------G-G---G-G--G-G-A-AC-A-CC---TGT----G--GC-GAA-G--G-C----G-----G--G-T-A-A---CTG-----G--GC-C-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-TA--C-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0017537 M01867_242_000000000_ATWYL_1_2103_24923_6139 .CAA--G-A-A--TA-TT--CC-G-C------AA-T-G-G--AC---------------------------GA-A--A--G-T--C-T-G-A-C-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-A-G--G--GAT-------------------------T-G--A--A--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-C-TC--------------------------TT-TT-C-T--G-CGG----G--A-A--G---AA-TAAG-T---C-T-G--G-T--A-------------------G--G----------------------------GA--------------------------------A--------T---------------GA---C-CAG-A-TG-A-T-G-A-C-G-----G-T---A-C-TG--------C-A-G-G---------A-A-----------T--AA-------------GC-C--CC-G---G-C-TAA-----------------------T--T-A-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GT-AG-GGG------------------GCA-A-G------------C--G--T---T--GC-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-TT-------G-TA-G-G-C-G---------------G--C-CC-A-T-C----------------------AA----G-T-C-G------------A-A-T--G--TA--A-AA-AA--C-CACT-G-G-----------------------------------------------------CT-C-AA-----------C-T-G-T-G-G-G--G-A----T-G--C-G---T--T--------------C--GA-A-A---C---------------T--G-G--TG--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--C-----T-GG------CG-G-G--------------G-G-G-T---AA-T--------------GG--A--ATT-----------C-C-C-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-G---G-A--G-G-A-AC-A-CC----GA-A--G--GC-GAA-G--G-C----A-----G--T-T-A-T---CCA-----T--GC-T-A------------------------------------------------------------------------------A-G------A-C-T--GA--CG---C----------------------------T-G--A-GG--A-G-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--CA

Otu0006650 M01867_242_000000000_ATWYL_1_1111_19025_13065 .TTC--G-A-A--TT-AT--TC-A-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-T-G-G-T---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--G--GA--------------------------T-G--A--A--G-G-TC---------CT-TG-------G-A-T--------T-G-T--A---AA-C-C-CC--------------------------TG-TC-A-C--C-GGG----G--A-A--G---AA-AAAG-T---G-C-A--T-T--T----------------------T----------------------------AA--------------------------------T---A--------------------GA---A-TGT-A-CT-C-T-G-A-C-T-----T-A---A-C-CC--------G-G-A-G---------A-G-----------G--AA-------------GC-A--GT-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-ACT------------------GCA-A-G------------C--G--T---T--AT-T-CGG-AT------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-CA-G-G-C-G---------------G--C-CA-G-G-T----------------------GT----G-T-T-A------------G-G-C--G--TG--A-AA-GC--C-C-GT-C-G-----------------------------------------------------CT-C-AA-----------C-G-A-C-G-G-A--A-T----T-G--C-A---C--C--------------T--AA-A-A---C---------------T--A-T--CT--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T--A-----C-TG------GA-G-A--------------G-G-G-T---AG-T--------------GG--A--ATT-----------C-A-C-G-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------G-T---G-A--G-G-A-AC-A-CC---AAT----G--GC-GAA-G--G-C----A-----A--C-T-A-C---CTG-----G--AC-A-G------------------------------------------------------------------------------T-T------A-C-T--GA--CG---C----------------------------T-C--A-GG--C-A-CG-A--AA-G-C----G-TG--GG-G--AG-C-A-AA--AG

Otu0001461 M01867_242_000000000_ATWYL_1_1110_23999_10819 .CGA--G-A-A--TT-TT--TC-A-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-T-G-G-A---GC-G-----A--C-GCC-G-C-G-T-----G-G-G--G--GA--------------------------T-G--A--A--T-G-GC---------TT-CG-------G-C-C--------C-G-T--A---AA-C-C-CC--------------------------TG-TC-A-T--T-TGC----G--A-T--C---AA-ACCT-C---A-C-G--A-C----------------------C--T----------------------------AA--------------------------------C---A----C----------------G---T-CGT-G-AG-T-T-G-C-T-A-----G-T---A-G-CG--------G-A-A-G---------A-G-----------G--AA-------------GG-G--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTC------------------CCA-A-G------------C--G--T---T--GT-T-CGG-AT------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GT-GC-------G-TA-G-G-C-G---------------G--T-CG-G-G-T----------------------AA----G-T-C-T------------G-A-C--G--TG--A-AA-TC--T-T-CA-A-G-----------------------------------------------------CT-C-AA-----------C-T-T-G-A-A-A--A-C----G-G--C-G---T--C--------------G--GA-T-A---C---------------T--A-T--TT--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-C-G-A-G-G--A-----A-TG------GA-G-G--------------G-G-A-G---AC-T--------------GG--A--ATA-----------C-T-T-G-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-AG--AT-A-TC-----------------------------A-A---G-T--G-G-A-AC-A-CC----AG-C--G--GC-GAA-G--G-C----G-----A--G-T-C-T---CTG-----G--AC-A-T------------------------------------------------------------------------------T-T------C-C-T--GA--CG---C----------------------------T-G--A-GG--C-A-CG-A--AA-G-C----C-AG--GG-G--AG-C-A-AA--CG

Otu0003341 M01867_242_000000000_ATWYL_1_1108_17200_20266 .CGA--G-G-A--TC-AT--TC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-A-T---GC-G-----A--C-GCT-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--C--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-CC--------------------------TG-TC-A-T--G-CGG----G--A-A--A---AA--CGG-A---C-C----T-G--C-G-----------------A--C----------------------------AA--------------------------------C---A----T-------------C--A---A-GGG-A-AC-G-G-G-A-T-G-----G-T---A-C-CG--------C-A-A-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-CG-C-G-T----------------------GT----G-T-G-G------------G-G-T--G--TG--A-AA-TC--T-C-CG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-G-G-A-A--A-G----T-G--C-G---C--C--------------C--CA-A-A---C---------------T--G-C--GC--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--G-----C-GG------GA-G-G--------------G-G-A-G---AT-C--------------GG--A--ATG-----------C-A-G-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-TG--AT-A-TC-----------------------------C-T---G-C--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--A-T-C-T---CTG-----G--AA-C-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----A-GG--GG-G--AG-C-A-AA--CA

Otu0003551 M01867_242_000000000_ATWYL_1_1112_14257_16048 .CGA--G-G-A--TC-AT--TC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-A-T---GC-G-----A--C-GCT-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--C--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-CC--------------------------TG-TC-A-T--G-AGG----G--A-A--A---A--ACGA-A---C-C-T--G-C--G-------------------A--C----------------------------AA--------------------------------C---A----T---------------CA---A-GGG-G-AC-G-G-G-A-T-G-----G-T---A-C-CT--------C-A-A-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-GC-G-G-T----------------------GT----G-T-C-G------------G-G-T--G--TG--A-AA-TC--C-A-TG-G-G-----------------------------------------------------CC-C-AA-----------C-C-C-A-T-G-A--A-G----T-G--C-G---C--C--------------C--GA-A-A---C---------------T--G-C--CG--C-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--G-----C-GG------GA-G-G--------------G-G-A-G---AT-C--------------GG--A--ATG-----------C-A-G-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-TG--AT-A-TC-----------------------------C-T---G-C--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--A-T-C-T---CTG-----G--AA-C-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-T----C-GG--GG-G--AG-C-A-AA--CA

Otu0008445 M01867_242_000000000_ATWYL_1_2108_20097_15424 .CGA--G-G-A--TC-AT--TC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-A-T---GC-G-----A--C-GCT-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--C--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-CC--------------------------TG-TC-A-T--G-GGG----G--A-A--A---A--AGGG-A---C-C-T--G-A--G-------------------A--C----------------------------AA--------------------------------C---A----T---------------CA---A-GGG-A-AC-C-G-G-A-T-G-----G-T---A-C-CC--------C-A-A-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-GC-G-G-T----------------------GT----G-T-C-G------------G-G-T--G--TG--A-AA-TC--C-C-AG-G-G-----------------------------------------------------CC-C-AA-----------C-C-C-T-G-G-A--A-G----T-G--C-G---C--C--------------C--GA-A-A---C---------------T--G-C--CG--C-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T--G-----C-GG------GA-G-G--------------G-G-A-G---AT-C--------------GG--A--ATG-----------C-A-G-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-TG--AT-A-TC-----------------------------C-T---G-C--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--A-T-C-T---CTG-----G--AA-C-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----A-GG--GG-G--AG-C-A-AA--CA

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Chapter 3

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Otu0010778 M01867_242_000000000_ATWYL_1_1104_19701_20103 .CGA--G-G-A--TC-AT--TC-G-C------AA-T-G-G--GC---------------------------GA-A--A--G-C--C-T-G-A-C-G-A-T---GC-G-----A--C-GCT-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--C--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-CC--------------------------TG-TC-A-T--G-AGG----G--A-A--A---A--AGGG-A---C-C-T--G-A--G-------------------A--C----------------------------AA--------------------------------C---A----T---------------CA---A-GGG-A-AC-C-G-G-A-T-G-----G-T---A-C-CT--------C-A-A-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-CG-C-G-T----------------------GT----G-T-G-G------------G-G-T--G--TG--A-AA-TC--T-C-CG-G-G-----------------------------------------------------CT-T-AA-----------C-C-C-G-G-A-A--A-G----T-G--C-G---C--C--------------C--CA-A-A---C---------------T--G-C--GT--G-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-GG------GA-G-G--------------G-G-A-G---AT-C--------------GG--A--ATG-----------C-A-G-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-TG--AT-A-TC-----------------------------C-T---G-C--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--A-T-C-T---CTG-----G--AA-C-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-T----C-GG--GG-G--AG-C-A-AA--CA

Otu0018899 M01867_242_000000000_ATWYL_1_1108_13049_14171 .CGA--G-G-A--TC-AT--TC-G-C------AA-T-G-G--GG---------------------------GA-A--A--C-C--C-T-G-A-C-G-A-T---GC-G-----A--C-GCT-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--C--G-G-CC---------TT-CG-------G-G-T--------T-G-T--A---AA-C-T-CC--------------------------TG-TC-A-T--G-AGG----G--A-A--G---AG-GAGA-G---C---G--T-C--A-------------------C--G----------------------------AA--------------------------------C---A----C---------------TG---G-CGC-G-AT-T-T-G-A-C-G-----G-T---A-C-CT--------C-A-A-G---------A-G-----------G--AA-------------GC-C--AC-G---G-C-TAA-----------------------C--T-C-T--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---AG-AG-GTG------------------GCG-A-G------------C--G--T---T--GT-T-CGG-AT------TT-A--C-T--GG-GC-------------------GT--A-----AA-GG-GC-GC-------G-TA-G-G-C-G---------------G--C-GA-T-G-T----------------------GT----G-T-C-G------------G-G-T--G--TG--A-AA-TC--C-C-AG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-T-G-G-A--A-G----T-G--C-G---C--C--------------C--GA-A-A---C---------------T--G-C--AT--C-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T--G-----C-GG------GA-G-G--------------G-G-A-G---AT-C--------------GG--A--ATG-----------C-A-G-G-GT--GT-A-G-CG-GT--G--------A-A-A---TG-C-GT-TG--AT-A-TC-----------------------------C-T---G-C--A-G-A-AC-A-CC----GG-T--G--GC-GAA-G--G-C----G-----G--A-T-C-T---CTG-----G--AA-C-G------------------------------------------------------------------------------C-A------A-C-T--GA--CG---C----------------------------T-G--A-GG--C-G-CG-A--AA-G-C----C-GG--GG-G--AG-C-A-AA--CA

Otu0002263 M01867_242_000000000_ATWYL_1_1102_6774_12211 .CGA--G-A-A--TA-AT--TC-A-C------AA-T-G-G--GG---------------------------GC-A--A--C-C--C-T-G-A-T-G-G-T---GC-A-----A--C-GCC-G-C-G-T-----G-G-A--G--GA--------------------------T-G--A--C--G-G-TC---------TT-CG-------G-A-T--------T-G-T--A---AA-C-T-CC--------------------------TG-TC-A-T--C-CGG----G--A-G--T---AA-GACC-T---G-G-G--A-G--T----------------------T----------------------------AA--------------------------------T---A--------------------GC---T-CACAG-GG-T-T-G-A-T-A-----G-T---A-C-CG--------G-A-A-G---------A-G-----------G--AA-------------GG-G--AC-G---G-C-TAA-----------------------C--T-T-C--G-T---------G-C-CA--G-C---A--G-C--CG---C-----------------GG--TA-AT-------AC---GA-AG-GTC------------------CCG-A-G------------C--G--T---T--GT-T-CGG-AA------TC-A--C-T--GG-GC-------------------GT--A-----AA-GG-GA-GC-------G-TAGG---C-G---------------G--C-GT-G-G-T-----------------------AA---G-T-C-A------------G-A-T--G--TG--A-AA-TC--C-C-GG-G-G-----------------------------------------------------CT-C-AA-----------C-C-C-C-G-G-A--A-C----T-G--C-A---T--C--------------C--GA-T-A---C---------------T--G-C--CG--T-G-C-------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-G--A-----T-TG------GA-G-A--------------G-G-T-A---GC-T--------------GG--A--ATT-----------C-T-T-G-GT--GT-A-G-CA-GT--G--------A-A-A---TG-C-GT-GG--AT-A-TC-----------------------------A-A---G-A--G-G-A-AC-A-CT----CG-T--G--GC-GAA-A--G-C----G-----A--G-C-T-A---CTG-----G--AC-A-A------------------------------------------------------------------------------C-T------C-C-T--GA--CG---C----------------------------T-G--A-GG--C-T-CG-A--AG-G-C----C-AG--GG-T--AG-C-G-AA--AG

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Table S4. List of responding OTUs in chitin-amended microcosms that responded in GlcNAc-

amended microcosms as well. Shown is the number of significantly responding OTUs at the

respective phylum-level, for both 16S rRNA genes and 16S rRNA combined.

Phylum / Class OTUs that responded to chitin and GlcNAc amendment

Bacteroidetes 121, 312, 1780, 1871, 2159, 2784, 4457

Chloroflexi 209, 1154

Firmicutes 3707, 4716

Parcubacteria 4551

Cyanobacteria 433

Betaproteobacteria 498

Epsilonproteobacteria 567

unclassified Proteobacteria 9443

unclassified 683, 4762, 5777, 7045, 8653, 9937, 10349

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Chapter 4

The active sulfate-reducing microbial community in littoral sediment of

oligotrophic Lake Constance

Susanne Wörner and Michael Pester

Published in Frontiers in Microbiology (2019) 10:247.

Abstract

Active sulfate-reducing microorganisms (SRM) in freshwater sediments are under-examined,

despite the well-documented cryptic sulfur cycle occurring in these low-sulfate habitats. In

Lake Constance sediment, sulfate reduction rates of up to 1,800 nmol cm-3 day-1 were

previously measured. To characterize its SRM community, we used a tripartite amplicon

sequencing approach based on 16S rRNA genes, 16S rRNA, and dsrB transcripts (encoding the

beta subunit of dissimilatory sulfite reductase). We followed the respective amplicon dynamics

in four anoxic microcosm setups supplemented either with (i) chitin and sulfate, (ii) sulfate

only, (iii) chitin only, or (iv) no amendment. Chitin was used as a general substrate for the

whole carbon degradation chain. Sulfate turnover in sulfate-supplemented microcosms ranged

from 38 to 955 nmol day-1 (g sediment f. wt.)-1 and was paralleled by a decrease of 90–100%

in methanogenesis as compared to the respective methanogenic controls. In the initial sediment,

relative abundances of recognized SRM lineages accounted for 3.1 and 4.4% of all bacterial

16S rRNA gene and 16S rRNA sequences, respectively. When normalized against the 1.4 × 108

total prokaryotic 16S rRNA gene copies as determined by qPCR and taking multiple rrn operons

per genome into account, this resulted in approximately 105–106 SRM cells (g sediment f. wt.)-

1. The three amplicon approaches jointly identified Desulfobacteraceae and

Syntrophobacteraceae as the numerically dominant and transcriptionally most active SRM in

the initial sediment. This was corroborated in the time course analyses of sulfate-consuming

sediment microcosms irrespective of chitin amendment. Uncultured dsrAB family-level

lineages constituted in sum only 1.9% of all dsrB transcripts, with uncultured lineage 5 and 6

being transcriptionally most active. Our study is the first holistic molecular approach to quantify

and characterize active SRM including uncultured dsrAB lineages not only in Lake Constance

but for lake sediments in general.

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Introduction

Lake Constance is a typical pre-alpine lake that has currently an oligotrophic status and is

oxygenated down to the sediment (Güde and Straile, 2016). Within the first millimeters of its

sediment, oxygen is completely depleted (Gerhardt et al., 2005) leaving room for anaerobic

degradation of organic matter either by fermenters that share their degradation products (acetate

and H2) with methanogenic archaea (Rothfuss and Conrad, 1993; Thebrath et al., 1993) or by

nitrate- (Hauck et al., 2001), Fe (III)- (Gerhardt et al., 2005), humic acid- (Kappler et al., 2004),

and sulfate-reducing microorganisms (Bak and Pfennig, 1991a,b). A major component of

organic matter entering the sediment is chitin as an abundant biopolymer in aquatic

environments (Gooday, 1990; Cauchie, 2002). It consists of linked amino-sugar subunits with

ß-1,4-N-acetyl-D-glucosamine as the monomeric structure and serves as structural element in

the exoskeleton of arthropods (e.g., zooplankton) and the cell wall of fungi and certain algae

and protozoa (Gooday, 1990). The estimated annual production of 28 × 106 tons by crustaceans

and insects in freshwater ecosystems and its efficient turnover in freshwater sediments (50–

75%, Beier and Bertilsson, 2013) indicate chitin as an important carbon and nitrogen source for

microorganisms in these habitats (Gooday, 1990; Cauchie, 2002).

In Lake Constance, sulfate concentrations of 50–300 μM can be measured in the upper layers

of the littoral sediments. Here, maximum sulfate reduction rates (SRR) as determined using 35S-

SO42- as a radiotracer reached values of 1,800 nmol cm-3 day-1 at 1–2 cm sediment depth (Bak

and Pfennig, 1991a). This surpasses typical SRR in sulfate-rich marine surface sediments by

one order of magnitude, where sulfate reduction is one of the major anaerobic carbon

degradation pathways (Jørgensen, 1982; Bowles et al., 2014). The high SRR at concomitant

low sulfate concentrations in Lake Constance are explained by a fast and effective cycling of

sulfur species between their oxidized and reduced states, which is controlled by a cryptic sulfur

cycle as is typical for freshwater sediments and wetlands (Pester et al., 2012). In particular,

sulfide as the end product of sulfate reduction does not accumulate beyond 80 μM in littoral

sediments of Lake Constance (Gerhardt, 2004), but is likely rapidly re-oxidized back to sulfate

at oxic-anoxic interfaces or under completely anoxic conditions using nitrate, Fe (III), or humic

acids as electron acceptor (Pester et al., 2012). SRR in Lake Constance sediments are saturated

at 60 μM sulfate, reaching maximum values of 3,000 nmol cm-3 day-1 in sediment slurries (Bak

and Pfennig, 1991a). This shows that SRR are not limited by the prevailing sulfate

concentrations in Lake Constance (Bak and Pfennig, 1991a; Güde and Straile, 2016), which is

also true for many other freshwater lakes (Holmer and Storkholm, 2001).

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To date, SRM are known to belong to the five bacterial lineages Deltaproteobacteria,

Nitrospirae, Clostridia, Thermodesulfobiaceae, and Thermodesulfobacteria as well as the two

archaeal lineages Euryarchaeota and Crenarchaeota (Muyzer and Stams, 2008). Using

metagenomics-guided discovery of novel microorganisms, three recent studies revealed the

capacity for dissimilatory sulfate or sulfite reduction in at least 13 additional bacterial and

archaeal lineages, which were so far not associated with this metabolic trait (Anantharaman et

al., 2018; Hausmann et al., 2018; Zecchin et al., 2018). These included among others the

Acidobacteria, Planctomycetes, Verrucomicrobia, and Armatimonadetes. In specific, 8 of these

13 newly identified SRM groups are candidate phyla without isolated representatives

(Anantharaman et al., 2018). In parallel, microbial diversity assays based on the functional

marker genes dsrAB, which encode the dissimilatory sulfite reductase and are commonly used

as diagnostic markers in detecting SRM, indicate an even larger phylogenetic diversity with at

least 13 uncultured family-level dsrAB lineages (Pester et al., 2012; Müller et al., 2015). So far,

the latter only partially overlap with the metagenome-discovered putative SRM, e.g., uncultured

dsrAB lineage 8 is represented by Acidobacteria (Hausmann et al., 2018) and uncultured dsrAB

lineage 13 is closely related to mesophilic Nitrospirae (Zecchin et al., 2018). Extending upon

the phylogenetic framework for dsrAB genes that was proposed by Müller et al. (2015), a recent

dsrB amplicon survey of 200 environmental samples including various freshwater habitats

increased the number of known species-level operational taxonomic units (OTUs) related to

potential SRM from ca. 800 (Müller et al., 2015) to more than 150,000 (Vigneron et al., 2018).

Considering the ca. 240 cultivated sulfate-reducing microbial species (Rabus et al., 2013), this

result uncovered that less than 0.2% of all potential SRM are available in pure culture. The

same study revealed that among uncultured SRM those belonging to uncultured dsrAB lineage

5 are quite prevalent in freshwater habitats (Vigneron et al., 2018).

Despite the importance of sulfate reduction for the sediment biogeochemistry of Lake

Constance as a typical freshwater lake, its SRM community is so far very poorly characterized.

The only attempt was based on enrichment cultures grown on H2, lactate, acetate, propionate

or long chain fatty acids, with isolates being affiliated to the genera Desulfovibrio,

Desulfobulbus, and Desulfotomaculum (Bak and Pfennig, 1991b). Therefore, we attempted to

systematically analyze and quantify the littoral SRM community of Lake Constance using a

tripartite, high throughput amplicon sequencing approach based on 16S rRNA genes, 16S

rRNA, and dsrB transcripts. To delineate active SRM, sediment was incubated in the presence

of chitin as a general substrate for the complete anaerobic degradation network in the presence

and absence of externally supplied pulses of sulfate. This was compared to sediment incubated

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with sulfate pulses only or without any external additions. Our results show that SRM constitute

about 3% of the total bacterial sediment community with their major representatives being

affiliated to the deltaproteobacterial families Desulfobacteraceae and Syntrophobacteraceae.

Results

Sulfate is readily turnedover in sediment microcosms

Twelve anoxic slurries of littoral sediment were pre-incubated for 7 days to allow for the

depletion of endogenous electron donors and acceptors. Among monitored short-chained fatty

acids and lactate, only acetate was detectable and accumulated transiently from 16 to 107 μM

(average across all incubations). Initial sulfate concentrations were 229 ± 34 μM and depleted

rapidly within the pre-incubation period to 24 μM (Figure 1), which indicated an active sulfate-

reducing microbial community.

Chitin supplementation resulted in a 3.6-fold higher accumulation of CH4 by day 43 under

methanogenic conditions as compared to the “control” (Figure 1). By contrast, in sulfate-

amended treatments, CH4 accumulation was suppressed by 90% in the chitin-amended

treatment as compared to the treatment with “chitin only” and suppressed completely in the

treatment “sulfate only.” This reflects the thermodynamically more favorable degradation of

organic matter by sulfate reduction (Muyzer and Stams, 2008) as compared to primary

fermentation or syntrophic processes coupling to methanogenesis (Schink and Stams, 2013).

Among the monitored degradation intermediates, only acetate could be detected in chitin-

amended microcosms, irrespective of the presence of external sulfate. However, the standing

concentrations of acetate were roughly three times higher under methanogenic conditions (142

± 41 μM) as compared to sulfate-reducing conditions (44 ± 30 μM) and converged only at the

end of the incubation toward a similar concentration of 109 μM. Parallel monitoring of CO2 in

the headspace of all incubations showed a steady accumulation over time, which was 5.0 and

2.5 times more pronounced in the incubations “chitin only” and “chitin & sulfate” as compared

to their respective controls (Supplementary Figure S2).

In all sulfate-amended treatments, sulfate turnover could be measured throughout the

incubation period. In the treatment “chitin & sulfate,” sulfate turnover rates ranged between 75

and 304 nmol day-1 (g sediment f. wt.)-1. This resulted in a total of 5.3 mM sulfate being turned

over. In the treatment “sulfate only,” sulfate turnover ranged from 38 to 281 nmol day-1 (g

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sediment f. wt.)-1 with a sudden increase to 955 nmol day-1 (g sediment f. wt.)-1 at the very end

of the incubation period. This resulted in a total of 6.0 mM sulfate being turned over (Figure

1). The measured bulk sulfate turnover rates were in the range of gross SRR observed

previously for the same littoral area (Bak and Pfennig, 1991a) and thus provided a good

experimental framework to detect active SRM under in situ-like conditions.

Figure 1: Time course of sulfate turnover and formation of metabolic end products in incubated

sediment microcosms. The gap illustrates the shift from pre-incubation to the chitin and/or first

sulfate amendment. Sulfate was always measured twice: before and after supplementation.

Opening of microcosms under anoxic conditions for sediment sampling is indicated by black

arrows. The mean and one standard deviation are shown, n = 3. Some error bars are smaller

than the symbol size.

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Sulfate amendment altered a minor portion of the bacterial community structure over time

The initial bacterial community of the analyzed sediment was dominated by the phyla

Proteobacteria (classes Alpha-, Beta-, Gamma-, and Deltaproteobacteria), Bacteroidetes,

Chloroflexi, Actinobacteria, Acidobacteria, Verrucomicrobia, Planctomycetes, Aminicenantes,

Cyanobacteria/Chloroplast, Ignavibacteriae or unclassified bacteria, with each of these phyla

constituting more than 1% relative abundance of the detected 16S rRNA genes (Supplementary

Table S1). Good’s coverage estimator (Good, 1953) of the sampled OTUs at 97% sequence

identity had an average of 0.88 ± 0.02, with 90% of all samples having a coverage between 0.86

and 0.90 (5 and 95% quantiles, respectively). At a rarefied sequencing depth of 68,826 reads

per replicate, the initial sediment harbored on average 14,190 ± 366 OTUs (mean ± SD), which

slightly decreased to 12,701 ± 595 OTUs (mean ± SD) in the different treatments over time

(Supplementary Figure S3). Total bacterial 16S rRNA gene copies stayed stable throughout a

period of 21 days of incubation and decreased only slightly toward the end of the incubation

time in chitin-amended treatments irrespective of sulfate addition. On average 1.4 ± 1.2 × 108

16S rRNA gene copies could be recovered per gram sediment (f. wt.) (Supplementary Figure

S4).

The response toward treatment and over time was monitored on both the 16S rRNA gene and

16S rRNA level as proxies for changes in relative bacterial population sizes and transcriptional

activities, respectively. Changes in beta diversity were visualized by a PCoA based on the

weighted unifrac metric as distance measure, which takes the phylogenetic relatedness as well

as the relative abundance of OTUs into account (Lozupone et al., 2011). Bacterial communities

separated mainly over time and to a minor extent based on treatment, both at the 16S rRNA

gene and 16S rRNA level (Figure 2). These shifts were significant (p-value < 0.01) as

determined by a PERMANOVA analysis, where 37.5 and 39.1% of the variation in bacterial

community compositions was explained by the incubation time at the level of 16S rRNA genes

and 16S rRNA, respectively. Variation due to incubation type was also significant, but much

smaller (12.5 and 12.4% for 16S rRNA genes and 16S rRNA, respectively). A significant

interaction between treatment and incubation time indicated that bacterial communities

responded differently over time depending upon the treatment, and this interaction explained

16.2 and 18.1% of the variation at the 16S rRNA gene and 16S rRNA level, respectively. When

correcting the 16S rRNA gene amplicon dataset for rrn copy numbers of the respective taxa, no

major differences were observed to the PCoA analysis and PERMANOVA without rrn operon

correction (Supplementary Figure S5).

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Figure 2. Time-resolved beta-diversity of bacterial communities in the various microcosm

setups according to a principal coordinate analysis (PCoA) based on the weighted unifrac

metric. Segregation of the bacterial community over time is shown for the 16S rRNA gene and

16S rRNA amplicon survey separately. Connected points of the same color represent biological

replicates (n = 3).

Desulfobacteraceae and Syntrophobacteraceae are the dominant SRM families in initial Lake

Constance sediment

In the initial sediment, relative abundances of recognized SRM lineages accounted for 3.14 ±

0.04 and 4.30 ± 0.06% of all 16S rRNA gene and 16S rRNA sequences, respectively, and were

comprised of seven different families, all affiliated to the Deltaproteobacteria (Figure 3). Both

types of sequencing analysis revealed Desulfobacteraceae (207 OTUs) and

Syntrophobacteraceae (86 OTUs) as the most abundant and transcriptionally active among all

recognized SRM families in the initial sediment. Members of the Desulfobulbaceae (34 OTUs),

Desulfomicrobiaceae (3 OTUs), Desulfovibrionaceae (22 OTUs), Desulfohalobiaceae (2

OTUs), and Syntrophaceae (31 OTUs; only Desulfobacca- and Desulfomonile-related)

represented in sum only 7.7 and 7.8% of 16S rRNA gene and 16S rRNA sequences related to

recognized SRM, respectively (Supplementary Table S2). Among the ten most abundant SRM-

related OTUs, seven had a relative abundance of >0.1%, which is typically taken as a cutoff to

delineate abundant microorganisms from the rare biosphere (Lynch and Neufeld, 2015). In

particular, those related to the dominating families Desulfobacteraceae and

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Syntrophobacteraceae accounted for 60.7 and 63.2% of all 16S rRNA gene and 16S rRNA

sequences affiliated with known SRM (Supplementary Table S2).

Figure 3. Community composition of recognized SRM families in the initial sediment and over

time in the various microcosm setups. For Syntrophaceae, only members of the genera

Desulfobacca and Desulfomonile were considered. Bars represent the mean relative abundance

for each treatment and time point (n = 3).

Over time, the 16S rRNA gene-based community composition of recognized SRM changed

only to a small extent in sulfate-amended treatments and stayed largely stable in the

methanogenic controls, irrespective of chitin amendment (Figure 3). Sulfate amendment in

general stimulated increases in Desulfobacteraceae (from 2.13 to 2.79%) and

Desulfobulbaceae (from 0.14 to 0.26%) 16S rRNA gene sequences. On the contrary, the

relative abundance of Desulfovibrionaceae increased only in the “chitin & sulfate” treatment

(from 0.01 to 0.18%). All other recognized SRM families showed no pronounced changes.

Responses toward the individual treatments were more pronounced at the 16S rRNA level

(Figure 3). While the relative amount of Desulfobacteraceae 16S rRNAs increased again under

“chitin & sulfate” (from 2.46 to 3.18%), they first increased and then dropped in the treatment

“sulfate only” and clearly declined in both methanogenic treatments. On the contrary,

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Desulfobulbaceae 16S rRNAs increased in all treatments, irrespective of chitin, and sulfate

supply. Interestingly, Syntrophobacteraceae revealed the most pronounced change, with their

relative 16S rRNA numbers being doubled in the treatment “chitin & sulfate” until the end of

the incubation time (from 1.17 to 2.19%) but staying largely stable in all other treatments. A

similar trend was observed for Desulfovibrionaceae, Desulfomicrobiaceae, and

Desulfohalobiaceae.

At the level of individual OTUs, 119 and 73 of a total of 397 SRM-related OTUs showed a

steady increase (R2 ≥ 0.80) within 21 days of incubation at the 16S rRNA level in the “chitin &

sulfate” and “sulfate only” treatments, respectively (Supplementary Table S2). For example,

these included the dominating Desulfobacteraceae OTU10 and Syntrophobacteraceae OTU30

in the “chitin & sulfate” treatment. At the 16S rRNA gene level, responses were less

pronounced with 84 and 88 SRM-related OTUs that showed a steady increase in the “chitin &

sulfate” and “sulfate only” treatments, respectively. Large relative abundance changes among

OTUs (logFC > 2.0; FDR-corrected p-value < 0.05) were restricted to a small subset of OTUs

steadily increasing in their relative 16S rRNA or 16S rRNA gene copies. The majority of these

OTUs belonged to the rare biosphere (<0.1% relative abundance) in the initial sediment and

affiliated mainly to the Desulfovibrionaceae and Desulfobacteraceae (Supplementary Table

S3).

Desulfobacteraceae and Syntrophobacteraceae dominate dsrB-transcribing microorganisms

The 16S rRNA (gene)-based analysis was supported by screening for microorganisms actively

transcribing their dsrB as a functional marker for SRM (Müller et al., 2015). Good’s coverage

estimator (Good, 1953) of the sampled dsrB cDNA OTUs in the initial sediment had an average

of 0.93 ± 0.05 with 90% of all samples having a coverage between 0.82 and 0.97 (5 and 95%

quantiles, respectively). Consistent with the data of the bacterial 16S rRNA gene and 16S rRNA

analyses, Desulfobacteraceae and Syntrophobacteraceae were the most abundant families

detected by dsrB cDNA amplicon sequencing (Table 1). Almost half of the dsrB-transcribing

microorganisms were represented by these two families in the initial sediment, of which the

most abundant dsrB OTU1 (Syntrophobacteraceae) and dsrB OTU2 (Desulfobacteraceae)

accounted for 12.3 and 11.6% of all dsrB transcripts, respectively (Supplementary Table S4).

In addition, results of the initial community of Lake Constance sediment revealed that most of

the 14 detected dsrB-transcribing family lineages were affiliated with the Deltaproteobacteria

supercluster (93.96% of all sequences, Table 1). Only 1.64 and 0.09% affiliated to the

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Firmicutes group and the Acidobacteria [formerly environmental supercluster, (Hausmann et

al., 2018)], respectively. Among uncultured family-level lineages, lineage 6 and 5 (both

Firmicutes group) were the most dominant ones and represented 1.36 and 0.24% of the dsrB-

transcribing community.

Table 1. Overview of the dsrB-transcribing community in initial littoral sediment of Lake

Constance. Classification was performed according to Müller et al. (2015). The mean and one

standard deviation of the relative abundance are given for n = 12.

Relative abundance Number

Reductive bacterial type DsrAB Family (%) of OTUs

Deltaproteobacteria supercluster Desulfobacteraceae 23.44 ± 1.03 39

Syntrophobacteraceae 22.36 ± 0.56 40

Desulfobacca acetoxidans lineage 12.46 ± 0.79 49

Desulfobulbaceae 8.99 ± 0.96 28

Thermodesulfobacteria 2.4 ± 0.18 3

Desulfonatronumaceae 1.49 ± 0.06 13

LA-dsrAB Firmicutes 0.39 ± 0.05 10

Desulfovibrionaceae 0.24 ± 0.03 4

Uncultured family-level lineage 11 0.22 ± 0.05 4

Desulfomicrobiaceae 0.03 ± 0.01 2

Desulfomonile tiedjei lineage 0.02 ± 0.02 1

Unclassified 21.92 ± 0.78 138

Environmental supercluster / Uncultured family-level lineage 8 0.05 ± 0.01 2

Acidobacteria Uncultured family-level lineage 9 0.04 ± 0.02 2

Firmicutes group Uncultured family-level lineage 6 1.36 ± 0.1 8

Uncultured family-level lineage 5 0.24 ± 0.04 15

Unclassified 0.04 ± 0.01 3

Unclassified Unclassified 4.3 ± 0.43 72

Similar to the results of the 16S rRNA analysis, the amendment of chitin and sulfate led to a

strong increase in the relative abundance of Syntrophobacteraceae dsrB transcripts, which more

than doubled from 23.1 to 57.9% after 21 days (Figure 4). Also in the treatment “sulfate only,”

the relative abundance of Syntrophobacteraceae dsrB transcripts increased transiently from

24.2 to 36.1% during the first 21 days but declined thereafter again toward 22.2%. On the

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contrary and in disagreement with the 16S rRNA analysis, Desulfobacteraceae dsrB transcripts

strongly decreased in relative abundance in the treatment “chitin & sulfate.” The opposite trend

was observed in the methanogenic “chitin only” and “control” treatments, where the relative

numbers of Syntrophobacteraceae dsrB transcripts decreased and Desulfobacteraceae dsrB

transcripts increased. Increased relative abundances of transcribed dsrB were also observed for

minor dsrB transcribing lineages. These comprised members of the Desulfomicrobiaceae,

Desulfovibrionaceae, Thermodesulfobacteria and Desulfobacca acetoxidans-lineage, with the

increase in relative dsrB transcript numbers being more pronounced in the treatment “chitin &

sulfate” than in “sulfate only” (Figure 4).

Figure 4: Relative abundance of dsrB transcripts affiliated to dsrAB family-level lineages

according to Müller et al. (2015). Bars represent the mean relative abundance of each lineage

for the respective treatment and time point (n = 3).

The OTU composition of dsrB-transcribing microorganisms was dominated by 16 dsrB OTUs,

each representing more than 1% of dsrB transcripts and accounting together for 62.3% of the

total relative abundance of dsrB transcripts in the initial sediment (Supplementary Table S4).

In particular, these dsrB OTUs were affiliated to the Desulfobacteraceae (OTU2, OTU5),

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Syntrophobacteraceae (OTU1, OTU7, OTU8), Desulfobacca acetoxidans lineage (OTU4,

OTU10, OTU11), Desulfobulbaceae (OTU12, OTU14, OTU27), Thermodesulfobacteria

(OTU6) and unclassified Deltaproteobacteria (OTU3, OTU13, OTU15, OTU19). Among these

highly transcriptionally active OTUs, dsrB OTU1 (Syntrophobacteraceae) was outstanding

since it increased more than threefold in relative dsrB transcript numbers in the “chitin &

sulfate” treatment where it comprised 39% of all detected transcripts at the end of the

incubation. Similarly, dsrB OTU1 comprised 26% of all dsrB transcripts at day 21 in the

treatment “sulfate only” and declined thereafter to a level similar to the initial sediment (15%

of all dsrB transcripts). In contrast, dsrB OTU1 strongly declined in the methanogenic “chitin

only” and “control” treatments, which was counterbalanced by a strong increase in dsrB

transcripts of Desulfobacteraceae dsrB OTU2 (up to 26% of dsrB transcripts).

Discussion

Sulfate reduction in littoral sediments of Lake Constance was estimated to contribute about

25% to the anaerobic degradation of organic matter during its eutrophic phase in the second

half of last century (Bak, 1988). In comparison to oligotrophic lakes, the respective SRR of up

to 20 mmol sulfate m-2 d-1 are in the upper range but are comparable to rates observed in other

mesotrophic and eutrophic lakes (Holmer and Storkholm, 2001). This importance of sulfate

reduction for limnetic sedimentary processes is reflected in the pronounced relative abundance

of SRM (3% of all bacterial 16S rRNA gene sequences, Figure 3), even in the currently

oligotrophic status of the lake. When normalized against the ca. 108 16S rRNA gene copies of

the total microbial community per gram sediment (f. wt.) (Supplementary Figure S4) and taking

into account the varying amount of rrn operons (1–21) on the genomes of different microbial

species (Stoddard et al., 2015) and the dominance of bacteria over archaea (Schwarz et al.,

2007), this would result in roughly 105–106 SRM cells per gram sediment. Our results compare

well to cultivation-based abundance estimates of SRM in littoral sediments of Lake Constance,

which detected 6 × 106 cultivable cells per ml of sediment (Bak and Pfennig, 1991b). In

addition, they correspond well to 16S rRNA gene-based estimates of SRM in sediments of other

lakes, such as eutrophic Lake Vechten (ca. 3%, Diao et al., 2018), mesotrophic Lake Kizaki

(ca. 4%, Li et al., 1999), meso-eutrophic Lake Kinneret (5–9%, Schwarz et al., 2007), or several

water reservoirs of oligotrophic to eutrophic status (ca. 3–5%, Wobus et al., 2003).

The difficulty in delineating active SRM in environmental surveys is their polyphyletic

distribution in the tree of life (Rabus et al., 2013) and the fact that many putative SRM are only

known by their dsrA/B sequences (Müller et al., 2015) or their dsrAB-carrying metagenome-

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assembled genomes (Anantharaman et al., 2018; Hausmann et al., 2018; Zecchin et al., 2018).

To address this methodological problem, we used a tripartite amplicon sequencing approach of

16S rRNA genes, 16S rRNA, and dsrB transcripts as proxies for relative population abundance,

general transcriptional activity, and activation of the sulfate reduction pathway, respectively.

This tripartite approach was applied to lake sediment microcosms that actively consumed

sulfate in the presence or absence of chitin as an abundant aquatic polysaccharide (Gooday,

1990) and general substrate for the whole organic carbon degradation network. All three

amplicon approaches jointly identified members of the Desulfobacteraceae and

Syntrophobacteraceae as the dominant SRM populations in the initial littoral sediment of Lake

Constance. Both SRM groups not only dominated in terms of relative abundance but also in

their general and dsrB transcriptional activity among both recognized SRM and in comparison

to uncultured dsrAB family-level lineages (Figure 3 and 4). This was corroborated in the time

course analyses of sulfate-consuming sediment microcosms irrespective of chitin amendment.

Here, Desulfobacteraceae showed clear population and general transcriptional increases

whereas Syntrophobacteraceae mainly responded by increasing their transcriptional activity

(Figure 3). The latter was clearly evident not only at the level of the 16S rRNA but also by the

respective dsrB transcripts. Especially one OTU, Syntrophobacteraceae-affiliated dsrB OTU1,

dominated relative dsrB transcript dynamics in sediment microcosms irrespective of chitin

amendment. It constituted either up to 26–40% of dsrB transcripts in sulfate-amended or less

than 1% in methanogenic microcosms toward the end of incubation (Supplementary Table S4).

It is very likely that these strong shifts in one OTU affected relative dsrB transcript dynamics

of other OTUs and lineages, which therefore have to be interpreted with caution. This is

especially true for a functional marker gene, where transcriptional dynamics in a few strongly

responding members are not stabilized by a majority of non-responding members as is often

the case in 16S rRNA amplicon surveys.

Our results compare well to studies, which analyzed the sulfate-reducing community of lake

sediments at the level of 16S rRNA genes only. As in littoral sediment of Lake Constance,

members of the Desulfobacteraceae constituted the largest fraction of sediment SRM in lakes

worldwide including Lake Geneva in Switzerland (Haller et al., 2011), Lake Biwa and Oko in

Japan (Watanabe et al., 2013), Lake Vechten in the Netherlands (Diao et al., 2018), and Lake

Kinneret in Isreal (Schwarz et al., 2007). While in Lake Constance and Lake Kinneret,

Syntrophobacteraceae were the second-most dominant SRM group (this study, Schwarz et al.,

2007), this position was taken by Desulfobulbaceae in other lakes (Watanabe et al., 2013; Diao

et al., 2018). Desulfobulbaceae were also detected in initial littoral sediment of Lake Constance,

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constituting the third most abundant SRM group (Figure 3) and being transcriptionally active

at the 16S rRNA and dsrB level (Figure 3 and 4). Placing the Desulfobulbaceae dsrB and 16S

rRNA sequences into the respective phylogenetic trees revealed that these OTUs were more

closely related to classical SRM such as members of the genera Desulfobulbus and

Desulfocapsa than to the recently described sulfide-oxidizing cable bacteria of the genera

Electrothrix and Electronema (Trojan et al., 2016).

From a physiological perspective, cultured representatives of the Desulfobacteraceae and

Syntrophobacteraceae are known to be metabolically versatile sulfate reducers with most

members being chemoorganotrophs that oxidize organic matter completely to CO2 (Kuever,

2014a,c). A fermentative lifestyle in the absence of sulfate is described for members of both

families, with members of the Syntrophobacteraceae being known to form syntrophic

associations with H2 or formate-utilizing partner microorganisms under such conditions

(Kuever, 2014a,c). Therefore, they seem to be well adapted to freshwater environments

characterized by a low sulfate content and steeply declining gradients of sulfate downward the

sediment (e.g., Bak and Pfennig, 1991a). In addition, Desulfobulbaceae accounted for the core

SRM community in the initial sediment. In contrast to Desulfobacteraceae and

Syntrophobacteraceae, members of this family are typically incomplete oxidizers of organic

compounds to acetate and CO2 (Kuever, 2014b). In the absence of sulfate, several members of

the Desulfobulbaceae are reported to use nitrate as a terminal electron acceptor (Kuever,

2014b), which may hint toward a different range of their ecological niche as compared to

Desulfobacteraceae and Syntrophobacteraceae. In addition, a few members of the

Desulfobulbaceae are even unable to use sulfate as terminal electron acceptor, but use

thiosulfate, sulfur or polysulfide instead or conserve energy by disproportionation of sulfur

compounds (Kuever, 2014b).

An important aspect of our study was that we screened not only for the presence and activity of

recognized SRM lineages but also included uncultured dsrAB lineages of putatively novel SRM

into our analysis. The used primer mixes DSR1728F-mixA and DSR4R-mix cover 89% of the

known sequence space of the phylogenetic branch of bacterial, reductively operating dsrAB

when allowing for one weighted mismatch (Müller et al., 2015). It was surprising to observe

that uncultured, family-level dsrAB lineages accounted for only 1.9% of all dsrB transcripts in

the initial sediment, with uncultured lineage 5 and 6 representing the most transcriptionally

active ones (Table 1). This was in contrast to freshwater wetlands, which constitute important

low-sulfate habitats for SRM as well. Here, typically uncultured lineages constitute a large

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fraction of the dsrAB diversity (Pester et al., 2012). One explanation could be that many of the

wetlands analyzed so far comprise acidic peatlands. The dsrAB-carrying community in these

habitats is dominated by members of uncultured lineage 8 (Loy et al., 2004; Steger et al., 2011;

Pester et al., 2012; Pelikan et al., 2016), which were recently described as Acidobacteria

encoding the complete pathway of dissimilatory sulfate reduction (Anantharaman et al., 2018;

Hausmann et al., 2018).

Conclusion

Lake Constance is one of best studied lakes worldwide and among the most important long-

term ecological research sites in limnology with data records over a hundred years period (Güde

and Straile, 2016). Its sediment microbiota has been extensively studied over the past decades

(see work by Norbert Pfennig, Ralf Conrad, Meinhard Simon, Bernhard Schink), yet a detailed

characterization of its SRM was missing. Our study is the first systematic approach to quantify

and characterize SRM including uncultured dsrAB lineages not only in Lake Constance, but for

lake sediments in general. Our results clearly demonstrated that members of the

Desulfobacteraceae and Syntrophobacteraceae dominate the SRM sediment community in

both abundance and transcriptional activity with uncultured dsrAB lineages contributing only a

minor proportion to the overall sulfate-reducing microbial community. Future research needs

to establish the individual ecological niches of the different SRM lineages identified.

Materials and Methods

Experimental set up

Sediment was taken from the littoral area of the Mainau Bay (Obere Güll) in Lake Constance

(N47°42′7′′ E9°11′43′′), Germany on September 15, 2015. Three sediment push cores were

sampled at 2 m water depth with plastic tubes of 80 mm inner diameter. At this shallow water

depth, the in situ temperature of the sediment is strongly influenced by the temperature of the

overlaying water column and can range from 4°C in winter (Bak and Pfennig, 1991a) to 23°C

in summer (Bak, 1988). The sediment structure and layering were preserved in the cores,

spanning a depth of 0–30 cm. Cores were covered with lake water and brought to the laboratory.

On the same day, the first centimeter was sliced off by a sterile metal plate from each core to

remove the oxygenated layer. The following two centimeters (1–3 cm below surface) were then

removed in one piece and immediately placed in a sterile anoxic tank, where the sediments of

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these cores were homogenized and divided into 12 sterile 150-mL glass bottles by 50 g portions

under a constant flow of 100% N2. 40 ml of anoxic, filter-sterilized (0.2 μm) surface lake water

was added to the sediment. All microcosms were sampled for their respective initial sediment

under a constant flow of 100% N2, sealed with butyl-rubber stoppers, and pre-incubated for

seven days at 15°C to deplete internal substrates within the sediment. Thereafter, microcosms

were split in triplicates into four incubation lines and further incubated at 15°C. Every 3–4 days,

we amended sulfate at dosages of 711 ± 539 μM to six of the microcosms. Three of these

received once 150 mg chitin (Sigma-Aldrich, Darmstadt, Germany) as a general and abundant

organic carbon source in aquatic sediments (incubation line 1: “chitin & sulfate”) as compared

to sulfate-stimulated control (incubation line 3: “sulfate only”). To control in parallel for

community members that are active in the methanogenic degradation network, the other six

microcosms were either incubated just with chitin (incubation line 2: “chitin only”) or without

any addition (incubation line 4: “control”) (Supplementary Figure S1).

Chemical analyses

Sulfate and degradation products of chitin were monitored every time incubation lines 1 and 3

were supplemented with sulfate. Gas samples from the headspace (200 μl) were monitored for

accumulation of CH4 and CO2 by gas chromatography (6000 Vega Series 2 GC, Carlo Erba,

Italy), using a 45/60 carboxen 1000 column (Supelco, Oberhaching, Germany) operated at

120°C and a thermal conductivity detector. 100% nitrogen gas was used as carrier gas at a

column pressure of 60 kPa. The injection port and detector were heated to 150 and 180°C,

respectively. Liquid samples (500 μl) were periodically taken either once for incubation lines

without sulfate supplementation (lines 2, 4) or before and after sulfate addition (lines 1, 3).

These samples were centrifuged (4°C, 14,000 ×g, 5 min) and the supernatant was stored at –

20°C until analysis. Before measurements, samples were centrifuged again (4°C, 14,000 ×g, 5

min) and the supernatant was used for analysis. Sulfate concentrations were determined

photometrically by a barium chloride turbidity test (Tabatabai, 1974). Formate, acetate,

propionate, butyrate, and lactate were monitored by HPLC (Shimadzu, Munich, Germany)

equipped with an Aminex HPX87H column (BioRad, Munich, Germany) heated to 45°C with

10 mM H3PO4 as eluent at a flow rate of 1 ml min-1. Analytes were detected with a photo diode

array detector at 200 nm (Shimadzu). The detection limit of all analyzed compounds was 10

μM.

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Sediment sampling and total nucleic acids extraction

Sediment in the microcosms was sampled at four different time points (Supplementary Figure

S1). First samples were taken right at the onset of the experiment and equaled the initial

sediment; all other samples were collected after 9, 21, and 43 days of incubation, which were

preceded by 7 days of pre-incubation. Sediment samples were frozen immediately in liquid N2

and stored at -60°C until further processing. RNA and DNA were extracted from the same

samples using the RNA PowerSoil® Total RNA Isolation Kit in combination with the RNA

PowerSoil® DNA Elution Accessory Kit (Mo Bio Laboratories Inc., Carlsbad, CA, United

States). Residual DNA in the RNA was removed with the TURBO DNA-free Kit (Ambion,

Thermo Fisher Scientific, Darmstadt, Germany). Transcription of RNA into cDNA was

performed using SuperScriptIII (Life Technologies, Darmstadt, Germany). To remove traces

of remaining RNA, extracted DNA was treated with RNase ONE (Promega, Mannheim,

Germany). RNA and DNA were quantified using Ribo- and PicoGreen (Life Technologies),

respectively.

Quantification of 16S rRNA gene copies

Quantitative PCR (qPCR) of total bacterial and archaeal 16S rRNA genes was performed on an

ABI 7500 cycler (Applied Biosystems) with the primer pair 1389F (5′-TGY ACA CAC CGC

CCG T-3′) and 1492R (5′-GGY TAC CTT GTT ACG ACT T-3’) as described in detail

previously (Hausmann et al., 2016). Besides an obligatory melting curve analysis, selected

qPCR products were visualized by agarose gel electrophoresis to verify absence of unspecific

PCR products. Amplification efficiencies had an average of 95 ± 4%. For qPCR assays a

standard curve with a purified 16S rRNA gene PCR product of an Acetobacteroides clone was

generated from 5 × 101 to 5 × 107 template copies per assay (R2 = 0.99). Absence of PCR-

inhibitory substances was confirmed by qPCR analyses of dilution series of two selected

sediment DNA extracts of this study.

Amplicon sequencing of 16S rRNA genes and cDNA

The V3–V4 region of bacterial 16S rRNA genes was amplified using the universal primer set

341F (5′-CCT ACG GGN GGC WGC AG-3′) and 805R (5′-GAC TAC HVG GGT ATC TAA

TCC-3′) (Herlemann et al., 2011). PCR amplification from DNA extracts and cDNA, barcoding

and Illumina amplicon sequencing was performed at Microsynth (Baldach, Switzerland). Initial

PCR amplification was performed by an annealing temperature of 56°C using 20 cycles. In a

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second PCR, barcodes were added to the initial amplicons using 10 cycles (DNA) or 8 cycles

(cDNA) at an annealing temperature of 56°C.

Amplicon reads were subjected to quality control and de novo chimera filtering (UCHIME,

Edgar et al., 2011) as implemented in Mothur v. 1.38.1 (Schloss, 2009). 5,362,183 high-quality

reads remained and formed 408,146 species-level OTUs (97% sequence identity). Taxonomic

identity was assigned with the RDP Classifier (Wang et al., 2007) and the RDP 16S rRNA

training set 16 using a confidence threshold of 0.8.

Illumina sequencing of dsrB cDNA

RNA extracts were reverse transcribed and subsequently amplified using a one-step RT-PCR

system (Access RT-PCR System, Promega). PCR amplification of dsrB-fragments (∼400 bp)

was performed according to a modified protocol described by Pelikan et al. (2016). The utilized

primers were DSR1728F mix (5′-CAY ACC CAG GGN TGG-3′) and DSR4R-mix (5′-GTR

WAR CAR TTD CCR CA-3′) (Steger et al., 2011) elongated by standard M13f (5′-CAG GAA

ACA GCT ATG AC-3′) and M13r (5′-GTA AAA CGA CGG CCA G-3′) primers, respectively.

75 ng RNA was reverse transcribed for 45 min at 45°C using only the reverse primer. After

cDNA synthesis, the forward primer was added and the cDNA was initially denatured for 2 min

at 94°C followed by 30 cycles of denaturation (95°C, 30 s), primer annealing (55°C, 30 s),

elongation (72°C, 30 s) and a final extension at 72°C for 7 min. PCR products were purified by

magnetic beads (Agencourt® AmpureXP, Beckman Coulter, Brea, United States). In a second

PCR, 1 μl of the purified PCR product was used as template targeted by M13-primers elongated

at each side with a 6-bp long barcode. Barcodes differed from each other by at least two

nucleotides. The M13 PCR was performed using 15 cycles of denaturation (95°C, 30 s), primer

annealing (60°C, 30 s), elongation (72°C, 40 s) and a final extension at 72°C for 5 min. PCR

products of correct length were purified with the MiniElute Gelextraction kit (Qiagen, Hilden,

Germany), quantified by PicoGreen, and mixed in equal amounts for sequencing on an Illumina

MiSeq machine.

Amplicon reads were quality controlled, checked for chimeras, and processed as described

above. 143,066 high-quality reads were obtained and formed 1,264 OTUs at the approximate

species-level (90% sequence identity). Taxonomic identity was assigned with the RDP

Classifier (Wang et al., 2007) and the dsrAB database described by Müller et al. (2015) using a

confidence threshold of 0.8.

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Statistical analyses

Alpha diversity among samples was compared by rarefying all replicates to an even sequencing

depth in Mothur v. 1.38.1 (Schloss, 2009). All other statistical analyses were performed in R,

version 3.3.2 (R Core Team, 2016), without rarefaction. Only OTUs that were detected by at

least three reads in three different sediment replicates were used in order to discriminate against

sequencing artifacts and to enable meaningful statistical analysis. Differences in bacterial

community composition were visualized using principal coordinate analysis (PCoA) plots made

in the R phyloseq package 3.3.2 (McMurdie and Holmes, 2013) as based on weighted unifrac

distances (Lozupone et al., 2011). Variation in community composition was tested for

significance using the weighted unifrac distances and a permutational analysis of variance

(PERMANOVA) in the R vegan package 2.5.1 (Oksanen et al., 2013). The package edgeR

3.16.5 (Robinson et al., 2010; McCarthy et al., 2012) was used to test for significant (FDR-

corrected p-value < 0.05) and large (logFC ≥ 2) changes of relative OTU abundances according

to both incubation time and incubation line. Corrections of the 16S rRNA gene amplicon dataset

for rrn operon numbers were performed at the highest taxonomic resolution of the obtained

OTUs as described before (Hausmann et al., 2016).

Sequence data availability

Amplicon data of 16S rRNA genes, 16S rRNA cDNA, and dsrB cDNA were deposited at the

Sequence Read Archive at NCBI (Leinonen et al., 2011) under the bioproject number

PRJNA495895.

Acknowledgments

We thank Sylke Wiechmann and Alfred Sulger for technical support, Ralf Müller for

establishing the dsrB amplicon approach in our laboratory, Muhe Diao (https://orcid.org/0000-

0002-0018-284X) for his analysis on the detailed phylogeny of Desulfobulbaceae dsrB

sequences, Bela Hausmann (https://orcid.org/0000-0002-0846-1202) for the correction of 16S

rRNA gene copies by the respective rrn operon numbers, and Bernhard Schink for his

continuous support.

Supplementary Material

The Supplementary Material for this article can be found online at:

https://www.frontiersin.org/articles/10.3389/fmicb.2019.00247/full#supplementary-material

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Chapter 5

General Discussion

In this thesis, the importance of sulfate-reducing microorganisms (SRM) in anaerobic organic

matter degradations in Lake Constance was investigated with a focus on chitin and its monomer

GlcNAc. To investigate chitin and GlcNAc degradation under naturally occurring conditions,

microcosms were set up under sulfate-reducing and methanogenic conditions over a period of

43 and 27 days, respectively. The identification of involved microorganisms during chitin and

GlcNAc degradation was investigated by 16S rRNA and 16S rRNA gene amplicon sequencing.

In addition, the SRM community in all treatments of the chitin incubation line was investigated

for their dsrB transcripts. In a side project, the SRM community was investigated by a stable

isotope probing study of rhizospheric rice paddy soil.

In anaerobic environments, the degradation of complex organic compounds starts with the

initial hydrolysis of the polymeric structure and is considered as the rate limiting step during

polymer degradation. Resulting mono-, di- and oligomers can then be imported into the cell and

degraded by high numbers of different microorganisms. Polymers such as chitin are insoluble

and have to be sensed and colonized before these can be used as carbon and electron source

(Beier and Bertilsson, 2013). The initial hydrolysis of polymer degradation is mediated outside

the cell, either by the release of enzymes in the surrounding environment or by cell-bound

enzyme complexes (Wilson, 2008). In general, the first step is performed by endoenzymes,

which break down the polymer into oligomeric structures of different length and have only a

restricted activity on soluble oligomeric (Henrissat et al., 1985). Exoenzymes act only on the

ends of polymeric fibers and profit therefore from the previous enzymatic activity of

endoenzymes by providing more free ends for these exo-acting enzymes. These exoenzymes

itself, can loosen branches of the polymeric compound, which in turn results in a better binding

of endoenzymes and may therefore explain the synergistic effect of endo- and exoenzymes of

polymeric structures (Eijsink et al., 2008).

In the analyzed lake sediment, the most responding phyla to chitin amendment are related to

Fibrobacteres (Chitinivibrio) and Firmicutes (Ruminiclostridium) (Chapter 3, Figure 4). Both

genera are known fibrolytic bacteria that require a firm attachment to the polymer (Wilson,

2008; Sorokin et al., 2014). It was shown that both fibrolytic members have different strategies

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to bind and use the polymeric compound, but both share the step of direct import of oligomeric

structures into the periplasm during polymer degradation. Exoenzyme activity, however, was

recognized mainly in the periplasm for both fibrolytic members, where the imported oligomers

are then further degraded. These strategies of polymer degradation may explain, why these

fibrolytic members have a reduced growth on short oligomers or the monomeric compound

(Wilson, 2008; Sorokin et al., 2014). In addition, both fibrolytic microorganisms are even

inhibited in their polymer degradation activity in the presence of high monomeric compounds.

By contrast, polymer degradation can be enhanced in the presence of non-fibrolytic

microorganisms (Koike and Kobayashi, 2009).

Interestingly, these two main responding phyla are also the most important fibrolytic members

in other habitats such as the gut of herbivores or insects (Mikaelyan et al., 2014; Henderson et

al., 2015) and indicate that only a small number of dominant fibrolytic microorganisms are

present in nature. And although these fibrolytic microorganisms are already known in other

habitats, these are still not investigated in an oligotrophic or pH neutral habitat as Lake

Constance sediment. In addition, there are a few highly interesting so far unclassified

responding OTUs that showed a similar succession as the Chitinivibrio (OTU1250, OTU1950,

OTU2707, OTU4370, Chapter 3, Supplementary Table 2) with a high response in the early

phase of the succession. Furthermore, OTUs within the Bacteroidetes which could only be

classified to the phylum level and considering the fact that Fibrobacteres were previously

classified within this phylum, might represent OTUs important during chitin turnover as well

(OTU1265, OTU2159, Chapter 3 , Supplementary Table 2) (Gupta, 2004). The result of

additional fibrolytic members was reported for other habitats as well and the authors suggested

that the majority of the fibrolytic microbial community is not identified and investigated so far

(Koike and Kobayashi, 2009).

Additional OTUs responding to chitin amendment are probably only involved as oligomer-

utilizing members or in a second step when the polymer is partly depolymerized and therefore

easier accessible. This may be the case for Chloroflexi, Spirochaetes, Ignavibacteria and some

Bacteroidetes, which may have, if anything, exochitinase activity. But these different activities

may explain the succession of the different responding phyla, where Chitinivibrio showed a

high response already after nine days of incubation whereas members of the phyla

Ignavibacteria, Chloroflexi, Spirochaetes, Deltaproteobacteria (order Myxococcales),

Bacteroidetes and Clostridia other than Ruminiclostridium responded at later time points. The

late response of Ruminiclostridium OTUs may be a result of adaptation to the new substrate

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(which was also observed for cellulolytic Fibrobacter) (Chapter 3, Figure 4 and Supplementary

Table 2). The presence of these different phyla is likely rather a cooperation than a competition

to chitin, in which the non-fibrolytic microorganisms use hydrolysis products for growth and

prevent on the same time inhibition effects of the fibrolytic member (Koike and Kobayashi,

2009). But it remains unclear, if this saccharolytic community imported oligomeric structures

or if some members have exochitinase activity and take up dimeric compounds or if some

members are even capable to de-complex polymeric compounds but all profited from the

polymer chitin, resulting in a higher response than to the monomeric compound GlcNAc.

The responding microbial community in Lake Constance sediment to the chitin-monomer

GlcNAc was dominated by Proteobacteria and showed only few OTUs that responded also in

chitin-amended microcosms (Chapter 3, Figure 5 and Supplementary Table 3). Most of the

stimulated microorganisms on the rhizosphere compared to the bulk soil, were related to

Proteobacteria as well and indicates that mainly easily degradable substrates were released and

available for the microorganisms in this habitat. However, fermentation products were similar

during anaerobic degradation of organic matter in both habitats, but with a much higher

accumulation of acetate in GlcNAc-amended treatments. This indicates that the amount of free

available sugars is an exception in these environments and exceeded the capacity of the acetate-

oxidizing community. Under high H2 concentrations (partial pressure), many primary

fermenters are forced to switch their acetate-forming fermentation pathway to alternative

pathways, e.g. ethanol or longer organic acids to dispose the endergonic reaction under a partial

pressure of more than 10 Pa (Schink, 1997). The absence of measurable H2 in all treatments,

indicate therefore that the primary fermenters had mainly an acetate-forming pathway under

these conditions. In chitin-amended microcosms, acetate accumulated to lower values than in

GlcNAc-amended treatments but was still present in higher amounts as compared to the initial

sediment, indicating that acetate oxidation processes, by methanogens (Rothfuss and Conrad,

1993), sulfate (Bak and Pfennig, 1991a) or iron(III) (Kappler et al., 2004) reducers was lower

than polymer hydrolysis to reach initial occurring concentrations. But the low accumulation of

fermentation products showed a readily active microbial community that responded fast and

effective to the polymer chitin during all steps of anaerobic polymer turnover and indicate that

it is a usual substrate in this habitat.

Different primary fermenters (fibrolytic and saccharolytic) responded in chitin- and GlcNAc-

amended microcosms or in the rice paddy soil, but all these fermenting microorganisms did not

alter by the addition of sulfate. This indicates that the primary and initial carbon flow was

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General Discussion

83

independent from external sulfate supply and occurs under sulfate-reducing as well as under

methanogenic conditions. Fermentation products of these primary fermenting microorganisms

(fibrolytic or saccharolytic) are mainly acetate, H2 and CO2. To a lesser extent, ethanol,

propionate, succinate, glycerol, lactate and formate may be released as well (Paster and Canale-

Parola, 1982; Sorokin et al., 2014; Badalato et al., 2017). Although acetate was the main

measured fermentation product, sulfate-reducing microorganisms, as a metabolic group, could

in principle directly use all other fermentation products if temporarily more easily available

carbon becomes available (Muyzer and Stams, 2008).

In both habitats, lake sediment and rice paddy soil, the initial SRM community was dominated

by gram-negative Deltaproteobacteria of the families Desulfobacteraceae,

Syntrophobacteraceae and Desulfobulbaceae. Members of these families responded to the

addition of sulfate, independent of external carbon supply as revealed by 16S rRNA (gene)

amplicon sequencing in both habitats (Chapter 2, Figure 2 and Chapter 4, Figure 3). In Lake

Constance sediment, the active transcription of the phylogenetic marker gene dsrB was

observed and revealed the highest transcription in Syntrophobacteraceae (Chapter 4, Figure 4

and Table 1). Surprisingly, Desulfobacteraceae and Desulfobulbaceae showed reduced dsrB

transcript activity during the time course of 43 days. The enhanced 16S rRNA levels showed

that these two families profited from the amended sulfate and the concurrent decreased dsrB

transcription may therefore indicate that these families (or OTUs) were involved in alternative

processes within the cryptic sulfur cycle (Chapter 4, Figure 3 and 4). A possibility could be the

disproportionation or the oxidation of reduced sulfur species, which should be constantly

supplied in the sulfate-amended treatments. The use of reduced sulfur compounds was

previously observed by members of the genera Desulfobulbus and Desulfocapsa, to which the

responding Desulfobulbaceae-OTUs are affiliated in the present study (Chapter 4,

Supplementary Table 3). These genera were reported of being even unable to use sulfate as

terminal electron acceptor (Böttcher et al., 2005; Finster et al., 2013). Therefore, it would be

interesting to investigate their impact within this process probably by the investigation of active

reverse dsrAB, sulfide-quinone reductase (sqr), or the sulfur-oxidizing (sox) enzyme

transcription or their response to oxygen, nitrate or iron(III) in combination with reduced sulfur

compounds.

Desulfovibrio representatives responded in both habitats only, if external carbon sources were

supplied in addition. An explanation could be that these fast-growing Desulfovibrio-OTUs use

H2, which is released during fermentations (Schink, 1997). This lifestyle may also be

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General Discussion

84

responsible for the high number of isolates related to this genus although it counts only for a

low number of the total SRM community in Lake Constance sediment (Bak and Pfennig,

1991b). Therefore, this member of the SRM community in these habitats is thought to be of

minor importance in the cryptic sulfur cycle, since the environmental conditions occur much

likely under more restricted conditions as observed in the initial sediment.

Interestingly, basal information of the SRM community in Lake Constance sediment can be

observed in the initial sediment or the sulfate control treatment. First of all, a relatively abundant

sulfate-reducing community (4%) is present in the sediment and the identification also by 16S

rRNA (3%), revealed that this high number was metabolic active also under in-situ conditions

(Chapter 4, Figure 3 and Supplementary Table 2). Furthermore, sulfate seems to be constantly

present within this habitat (Chapter 4, Figure 1, (Bak and Pfennig, 1991a; b)) and the SRM

community did not change much under the addition of sulfate and/or chitin (Chapter 4, Figure

3 and 4), which in turn showed that this community is indeed constantly active and does not

have to be stimulated in this habitat for further investigations. The highly persistent SRM

community in the sulfate control treatment indicates that in addition a hidden carbon and

electron flow occurs in the sediment, which provides electron donors and carbon sources that

influence the cryptic sulfur cycle and have to be elucidated in future. A persistent community

was also observed in the methanogenic control, in which methanogenesis remained with the

same rate over the time course and let question arise, which electron donors/acceptors and

carbon sources are used by these methanogens or SRM to maintain the metabolic activity over

a period of 50 days. The drastic effect on methanogenesis by the surplus of sulfate in the sulfate

control showed that the turnover of internal substrates was highly affected by sulfate in the

microcosms but was not observed in the initial sediment.

In conclusion, the sulfate-reducing microbial community in Lake Constance sediment or rice

paddies are highly active under in-situ conditions. The amendment of sulfate increased sulfate

reduction in the presence or absence of external carbon supply. Deltaproteobacteria were the

main drivers of the reductive part of the cryptic sulfur cycle in these freshwater environments.

In the rice paddy, Desulfobulbaceae, Desulfobacteraceae, Syntrophobacter and Desulfovibrio

were identified as the most important members during sulfate reduction. In Lake Constance

sediment, Syntrophobacteraceae-members were the main drivers during sulfate reduction in

the sediment. Desulfobulbaceae and Desulfobacteraceae are considered as important members

in this cycle as well, either by sulfate reduction or during disproportionation or re-oxidation of

reduced sulfur compounds.

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Summary

85

Chapter 6

Summary

This thesis investigated the diversity of sulfate-reducing microorganisms (SRM) in freshwater

habitats. To understand their ecological role in these different habitats in more detail, these

microorganisms were investigated in relation to the natural occurring carbon flow in these

habitats. Here the different fermentation products were investigated that are released during the

anaerobic turnover of root exudates on the rhizosphere of rice plants or the turnover of the

polymer chitin in lake sediments, an abundant polymer in aquatic environments, and serve

potentially as important carbon source for SRM.

The microbial community that profited from released root exudates on the rhizosphere was

represented mainly by Alpha-, Beta-, Gamma- and Deltaproteobacteria compared to the

surrounding bulk soil. In the same habitat, stable isotope probing revealed that 13CO2, fixed by

the rice plant, was in parts released as labelled root exudates into the rhizosphere and a fast (one

day) 13C-labeling of emitted methane was detactable as well. In addition, Bukholderiales,

Acidobacteria, Geobacter (Deltaproteobacteria) and subsection IV Cyanobacteria were

identified as directly involved in the turnover of labelled root exudates by incorporated 13C-

labelled carbon into their 16S rRNA.

Anaerobic chitin degradation in lake sediment microcosms resulted mainly in an accumulation

of acetate, CO2 and CH4, whereas an accumulation of H2 was not observed. Chitin turnover

measured by released ammonium as indicator for chitin degradation, showed a turnover rate of

4.4 nmol chitin h-1 and (g sediment dry weight)-1 and was even higher than the chitin-hydrolysis

rates measured by the chitin analog MUF-DC in the anoxic sediment (0.08-1.1 nmol MUF-DC

h-1 and (g sediment dry weight)-1). Involved microorganisms during this chitin degradation were

identified by 16S rRNA (gene) amplicon sequencing and resulted in a succession of the

microbial community, with Chitinivibrio as the most important hydrolytic member in the early

phase (9 days). Intermediate responders were represented by the phyla Bacteroidetes,

Chloroflexi, Proteobacteria (order Myxococcales) and Spirochaetes (21 days) and are most

likely involved during the turnover of chitooligomers. In the late phase (43 days), Firmicutes

(Ruminiclostridium) dominated the chitin-hydrolyzing community. The microbial community

involved during the turnover of N-actylglucosamine (GlcNAc, chitin monomeric compound)

was dominated by Alpha-, Beta- and Gammaproteobacteria.

Page 90: Elucidating the identity and physiology of sulfate

Summary

86

In the two different habitats, the microbial community involved during carbon degradation did

not alter by the addition of sulfate and showed under both conditions the same primary

fermenting bacteria. Sulfate amendment to soil or sediment suppressed the total

methanogenesis by up to 99% and the release of labelled 13CH4 as well. The SRM community

in the initial rice paddy soil or lake sediment was dominated by Gram-negative

Deltaproteobacteria within the families Desulfobacteraceae, Syntrophobacteraceae,

Desulfobulbaceae and Desulfobacca acetoxidans-related and counted for ca. 3-4% of the total

microbial community in the initial Lake Constance sediment. These high abundant SRM

lineages were further stimulated by the addition of sulfate, independent of external carbon

supply. On the contrary, Desulfovibrionaceae responded only in the presence of carbon and

sulfate in both habitats. The metabolic active SRM community in lake sediments was in

addition investigated by the transcriptional activity of the phylogenetic marker gene dsrB and

revealed Syntrophobacteraceae as the main active SRM member in Lake Constance sediment.

In conclusion, this thesis provided insights into a well-balanced microbial food web during

anaerobic polymer degradation, in which known fibrolytic members were the most important

hydrolyzing bacteria in Lake Constance sediment. ln addition, this thesis showed an abundant

and metabolically active SRM community that was dominated by Deltaproteobacteria.

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Zusammenfassung

87

Chapter 7

Zusammenfassung

In dieser Dissertation wurde die Diversität der Sulfat-reduzierenden Mikroorganismen (SRM)

in Süßwasserhabitaten untersucht. Um ihre ökologische Rolle in diesen unterschiedlichen

Gebieten besser zu verstehen, wurden diese Mikroorganismen in Verbindung zu dem

Kohlenstofffluss in ihrem natürlichen Habitat untersucht. Hierbei wurden die

Fermentationsprodukte untersucht, die während des anaeroben Abbaus von Wurzelexsudaten

der Reispflanze oder während der Umsetzung des Polymers Chitin in Seesedimenten, das ein

häufig vorkommendes Polymer in aquatischen Gebieten ist, freigesetzt werden und potentiell

wichtige Nahrungsquellen für SRM darstellen.

Die mikrobielle Gemeinschaft, die von der Freisetzung der Wurzelexsudate profitierte war im

Gegensatz zum umgebenden wurzelfreien Boden, hauptsächlich durch Alpha-, Beta-, Gamma-

und Deltaproteobacteria repräsentiert. Im gleichen Habitat zeigte die stabile

Isotopenbeprobung, dass fixiertes 13CO2 in der Wurzelzone als markierte Wurzelexsudate

freigesetzt wurde und in kurzer Zeit (ein Tag) die 13C-Markierung auch im Methan detektierbar

war. Ferner wurden durch den Einbau von 13C-markierten -Kohlenwasserstoffen in die 16S

rRNA Mikroorganismen identifiziert, die direkt bei der Umsetzung von markierten

Wurzelexsudaten involviert waren und konnten den Bukholderiales, Acidobacteria, Geobacter

(Deltaproteobacteria) und Untergruppe IV der Cyanobacteria zugeordnet werden.

Der anaerobe Chitin-Abbau in Seesediment-Mikrokosmen resultierte hauptsächlich in der

Akkumulation von Acetat, CO2 und CH4, wobei keine Zunahme von H2 zu beobachten war.

Der Chitinumsatz wurde durch freigesetztes Ammonium als Indikator für Chitin-Abbau

gemessen und zeigte eine Umsatzrate von 4.4 nmol Chitin h-1 und (g Sediment

Trockengewicht)-1 und war höher als die Hydrolyseraten, die durch das Chitin-Analog MUF-

DC im anoxischen Sediment bestimmt wurden (0.08-1.1 nmol MUF-DC h-1 und (g Sediment

Trockengewicht)-1). Mikroorganismen, die and diesem Chitin-Abbau beteiligt waren, wurden

durch 16S rRNA (Gen) Amplikon-Sequenzierung untersucht und resultierte in einer Sukzession

der reagierenden mikrobiellen Gemeinschaft, wobei Chitinivibrio das wichtigste hydrolytische

Mitglied in der frühen Phase (9 Tage) war. Darauf folgten Vertreter der Bakterienstämme

Bacteroidetes, Chloroflexi, Proteobacteria (Ordnung Myxococcales) und Spirochaetes (21

Tage), die sehr wahrscheinlich eine Rolle während des Abbaus von Chitin-Oligomeren beteiligt

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Zusammenfassung

88

waren. Die späte Phase (43 Tage) wurde durch Clostridien (Ruminiclostridium) dominiert.

Dahingegen waren Alpha-, Beta- and Gammaproteobacteria die dominierenden Ordnungen,

die am Abbau von N-Acetylglucosamin (GlcNAc, Monomer von Chitin) beteiligt waren.

In beiden Standorten änderte sich die mikrobielle Gemeinschaft, die während des

Kohlenwasserstoffumsatzes involviert war nicht wesentlich durch die Zugabe von Sulfat. Das

zugegebene Sulfat zum Reisfeldboden oder dem Seesediment unterdrückte die allgemeine

Methanogenese um 99% sowie den Ausstoß von markiertem 13CH4 im

Reisfeldbodenexperiment. Die Sulfat-reduzierende Gemeinschaft im ursprünglichen

Reisfeldboden und Seesediment war durch Gram-negative Deltaproteobacteria der Familien

Desulfobacteraceae, Syntrophobacteraceae, Desulfobulbaceae und Desulfobacca acetoxidans-

Verwandten dominiert und repräsentierte ca. 3-4% der gesamten mikrobiellen Gemeinschaft

im ursprünglichen Sediment des Bodensees. Diese abundanten SRM-Linien wurden durch die

Zusage von Sulfat weiter stimuliert, unabhängig von einer externen Kohlenwasserstoffquelle.

Im Gegensatz dazu reagierten Desulfovibrionaceae ausschließlich bei der Zugabe von Sulfat

und einer externen Kohlenwasserstoffquelle in beiden Standorten. Die metabolisch aktive

SRM-Gemeinschaft im Seesediment wurde zusätzlich durch die transkriptionelle Aktivität des

phylogenetischen Markergens dsrB untersucht und zeigte Syntrophobacteraceae als die

transkriptionell aktivste Sulfat-reduzierende Familie im Bodenseesediment.

Zusammenfassend konnte gezeigt werden, dass ein gut ausgeglichenes, mikrobielles

Nahrungsnetz während des anaeroben Polymer-Abbaus besteht, in welchem bekannte Polymer-

hydrolysierende Bakterien den größten Einfluss im Bodenseesediment zeigten. Zusätzlich

wurde eine abundante und metabolisch aktive SRM Gemeinschaft bestimmt, die von

Deltaproteobakterien dominiert wurde.

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Record of Achievement

89

Record of Achievement

My contribution to the manuscript in chapter 2 was the RNA extraction of already sampled and

frozen soil samples for stable isotope probing. Furthermore, I did the RNA stable isotope

probing, the 16S rRNA cDNA clone libraries and the T-RFLP analysis.

All experiments of the manuscripts in chapter 3 and 4 were developed and planned by myself

and my supervisor Prof. Dr. Michael Pester.

In chapter 3, gas measurements, sampling, HPLC-analysis and RNA/DNA extraction of

GlcNAc-amended microcosms were performed by Evgenia Isaeva during her Bachelor thesis

under my supervision.

Dominik Stickel did the chitinase activity tests during his Bachelor thesis under my supervision.

In Chapter 4, Bela Hausmann performed the correction of 16S rRNA gene copies by the

respective rrn operon numbers.

Muhe Diao analyzed the phylogeny of Desulfobulbaceae dsrB sequences in arb.

All other experiments in chapter 4 were done by myself.

Drafts for the manuscripts in chapter 3 and 4 were written by me and corrected by Prof. Dr.

Michael Pester.

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List of Publications

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List of Publications

Wörner, S., Zecchin, S., Dan, J., Todorova, N.H., Loy, A., Conrad, R., Pester, M. (2016).

Gypsum amendment to rice paddy soil stimulated bacteria involved in sulfur cycling but largely

preserved the phylogenetic composition of the total bacterial community. Environ. Microbiol.

Rep. 8(3), 413–423. doi: 10.1111/1758-2229.12413.

S. Wörner and M. Pester (2019). The active sulfate-reducing microbial community in littoral

sediment of oligotrophic Lake Constance. Front. Microbiol. 10(247).

doi: 10.3389/fmicb.2019.00247

S. Wörner and M. Pester (2019). The microbial succession of anaerobic chitin degradation in

freshwater sediments. Appl. Environ. Microbiol. (submitted).

Further Publications:

Borisova, M., Shi, Y., Buntru, A., Wörner, S., Ziegler, W.H., Hauck, C.R. (2013). Integrin-

mediated internalization of Staphylococcus aureus does not require vinculin. BMC Cell Biology

14(1), 2. doi: 10.1186/1471-2121-14-2.

Deutzmann, J.S., Wörner, S., Schink, B. (2011). Activity and diversity of methanotrophic

bacteria at methane seeps in eastern Lake Constance sediments. Appl. Environ. Microbiol.

77(8), 2573–2581. doi: 10.1128/aem.02776-10.