Effect of ivermectin onAnopheles aquasalis, a malaria vector

from South America

Marcus Lacerda MD, PhDFundação de Medicina Tropical Dr. Heitor Vieira Dourado

Instituto Leônidas & Maria Deane (FIOCRUZ-AM)

Effect on survival-6 volunteers (3 male and 3 female)-Blood sample (5mL)-100 mosquitos/sample/time-Observation at 24 hours intervals-Dead mosquitos removed and counted

METHODSPatient diagnosed with

helminthiasis with ivermectin indication

D0 D2 D4 D7

- IC signature- New sample after 4h

- Patient returns for new samples

D14

Analysis including all the 6 volunteers samples (males and females)

P < 0.001

RESULTS

14 days post-ingestion effect

- LC50 calculation- Evaluate the effect on the flight capacity- Evaluate the effect on the reproductive capacity- Evaluate the effect on the mosquito infection (P. vivax)- Verify the in vitro effect on P. vivax- Verify the mosquito gene expression profile under

ivermectin effect- Perform the same studies with Anopheles darlingi (from

the field), the major vector in South America- Evaluate ivermectin to massive drug administration

(MDA) trials under discussion in the Brazilian Amazon

FUTURE PERSPECTIVES