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Ecology of free-living Symbiodinium
Misaki Takabayashi, PhD
Lisa Adams (Master’s Candidate)Marine Science Department
University of Hawai`i at Hilo
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
Research at UH-Hilo
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
Photographs by John Oshima, UHH Graphics
Research at UH-Hilo• Facilities and equipment improvements with
EPSCoR (NSF) other funds since 2002
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
Research at UH-Hilo• Master’s program: Tropical Conservation
Biology and Environmental Sciences
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
Lisa AdamsSea Grant Graduate Fellow
Ecology of Free-living Symbiodinium
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
85% of symbiotic invertebrates must acquire Symbiodinium from the environment
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
Fish Feces
Sediment
Water column
Released from corals
Free-living “Symbiodinium”
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
1. Are free-living Symbiodinium present in water column and marine sediments?
2. What is the diversity of Symbiodinium in these populations?
3. Are these populations viable, accessible, and utilized by asymbiotic invertebrates?
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
1. Are free-living Symbiodinium present in water column and marine sediments?
• Yes
• Coffroth et al. 2006 (sediment Florida Keys)• Manning and Gates in review (Hawaii)
2. What is the diversity of Symbiodinium in these populations?
In Progress with collaborators…
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
2. What is the diversity of Symbiodinium in these populations?
MHI Okinawa
NWHI Australia
Florida
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
3. Are these populations viable, accessible, and utilized by asymbiotic invertebrates?
• Needed asymbiotic coral larvae
• All Hawaiian corals have zooxanthellate larvae
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
But Okinawa has asymbiotic coral larvae!
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
Univ. of Ryukyus, OkinawaCOE Summer Program
NSF East Asia Pacific Summer Institute Graduate Fellowship for Lisa Adams
Preferential Uptake of Symbiodinium from Sediment and Water Column
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
Asymbiotic larvae of Acropora monticulosa
SW
Sed
Filtered Non filtered
No
Sediment
Control
FSW
SW
Sediment FSW&Sed SW&Sed
Larvae infected with Symbiodinium earlier and in greater proportions in sediment containing treatments than SW only
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
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Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
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Symbiodinium density higher in sediment containing treatments than SW only
3. Are these populations viable, accessible, and utilized by asymbiotic invertebrates?
Conclusions:• Symbiodinium in both SW and sediment are
viable.• Symbiodinium in sediment infect more A.
monticulosa larvae and earlier than those in water column.
• Adams, Cumbo, Takabayashi in prep.
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
Hawaii Tribune Herald front page April 29th, 2007
Ocean Day, HawaiiPacific Aquaculture and Coastal Resources Center
Image by Cody Chapin
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
Student Training
Collaborations
• Drs. Ruth Gates and Xavier Pichon (Edwin Pauley Workshop, HIMB)
• Dr. Selina Ward (University of Queensland)• University of the Ryukyus (COE Summer
Prog)
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
Future
• Genotypic diversity analyses
• Quantification of genotypes
• >2 papers published
• Lisa Adams’ Master’s degree
• Ocean Day, Hawaii 2008
Presentation IndexPresentation Index: Research at UH-Hilo - Background - Hypotheses - Results - Extension - Collaboration - Future
