Echo®

for Regulated Laboratories

BROCHUREVersion 2.0 | MARCH 2019

© 2019 LABCYTE INC. All rights reserved.

LABCYTE INC.170 Rose Orchard WaySan Jose, CA 95134 USA

Toll-free: +1 877 742−6548 | Fax: +1 408 747−2010

Echo® Acoustic Liquid Handling for Regulated LaboratoriesREDEFINE SCIENTIFIC INNOVATION WITH ACOUSTIC LIQUID HANDLING

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Echo® Liquid Handlers for Regulated Laboratories

Re-imagine Your Science and Research Bioassay development and deployment are challenging. Complex workflows and stringent success criteria require liquid handling solutions that offer

both the flexibility to meet unique workflow needs and the exceptional reproducibility the work demands.

Using an innovative, non-contact dispensing technology, Labcyte Echo® Liquid Handlers deliver the utmost flexibility with any-well to any-well transfers

and unparalleled accuracy and precision, even when working with nanoliter transfer volumes. Pooling large numbers of assay components and sample

randomization, commonly challenging for standard automated liquid handlers, are straightforward with Echo® Liquid Handlers.

Echo® Liquid Handlers are transforming how researchers approach their science delivering significant cost reductions both through assay

miniaturization and elimination of pipette tips.

BROCHURE | ECHO® LIQUID HANDLERS FOR REGULATED LABORATORIES

www.labcyte.com/echo-regulated

Inverted destination plate

Transducer(acoustic energy generator)

Source plate2.5 nL or 25 nLdroplet transfers

Fluid TYPE A

Fluid TYPE B

Fluid TYPE C

Fluid TYPE D

FIGURE 1: Acoustic droplet ejection - The transducer is positioned below the source microplate well and emits focused sound energy repeatably to the meniscus of the fluid to be transferred. A stream of 2.5 nL or 25 nL droplets (model dependent) is reliably ejected into a well of an inverted destination microplate.

Using Dynamic Fluid Analysis™,

Echo® Liquid Handlers use sound

energy to determine fluid composition,

fluid height, and the power needed to

eject a precise volume of fluid into a

destination well. Dynamic Fluid Analysis

adjusts transfer parameters in real-time

to compensate for changes in fluid

height (well volume) and fluid properties

without recalibration.

On-the-Fly Adjustments to changing fluid properties

• Real-time adjustment to changing fluid types, volumes, or properties without user intervention

• Repeatable transfer of complex reagent sets or inconsistent reagents

DYNAMIC FLUID ANALYSIS™

Innovative Technology and Unparalleled Performance Echo® Liquid Handlers rely on patented technologies and novel methodologies to change how liquid handling is used in applications throughout life

science. With Dynamic Fluid Analysis™ Technology and Acoustic Droplet Ejection, the Echo® Liquid Handler enables researchers to transfer a broad

spectrum of fluid types with a degree of flexibility and reliability not possible with traditional liquid handling technologies.

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LABCYTE INC.

www.labcyte.com/direct-dilution

With the ability to transfer low nanoliter volumes of liquid, Echo® Liquid Handlers can produce dose-response and standard curves free of the errors

typically found using traditional tip-based liquid handlers and serial dilution. Echo® Liquid Handlers create a series of dilutions by directly transferring

decreasing volumes of stock sample to individual assay wells. Since each concentration point along the dilution curve is created individually, instead of

serially, the risk of propagating errors along the curve is eliminated — a significant improvement to data quality.

Simultaneous movement of the acoustic transducer and destination

plate during transfer enables Echo® Liquid Handlers to transfer from

any source well to any destination well faster than traditional liquid

handling technologies that require time to transport samples from

source wells to destination wells and to change tips between transfers.

for a more reliable dilution of samples and reagents

for superior speed and flexibility

Direct Dilution

Any-Well to Any-Well Transfer

FIGURE 3: Examples of rapid cherry picking, sample pooling, and combination screening from any source well to any destination well, at any volume.

A1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

BCDEFGHIJKLMNOP

A1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

BCDEFGHIJKLMNOP

Destination Plate

Source Plate

A1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

BCDEFGHIJKLMNOP

A1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

BCDEFGHIJKLMNOP

Source Plate

Destination Plate

Potency Assays with Direct Dilution

Dir

ect

Dilu

tio

n

Direct Dilution

% In

hib

itio

n

Inhibitor Concentration (M)

100

80

60

40

20

0

7.5 nL25 nL

250 nL

2.5 nL

10 mM Stock

2.5 nL

30 nL92.5 nL100 µM Intermediate

10 nL 2.5 nL

37.5 nL115 nL1 µM Intermediate

12.5 nL 5 nL

1E-10 1E-09 1E-08 1E-07 1E-06 1E-05

FIGURE 2: Example of an 11-point curve created using direct dilution. The image on the left shows how the desired concentrations are created by transfers directly from the stock sample and two intermediate dilutions of the stock sample. The resulting IC50 curve is shown on the right.

Direct Dilution

Potency Assays with Direct Dilution

Dir

ect

Dilu

tion

Potency Assays with Direct Dilution

Dir

ect

Dilu

tio

n

Direct Dilution

% In

hib

itio

n

Inhibitor Concentration (M)

100

80

60

40

20

0

7.5 nL25 nL

250 nL

2.5 nL

10 mM Stock

2.5 nL

30 nL92.5 nL100 µM Intermediate

10 nL 2.5 nL

37.5 nL115 nL1 µM Intermediate

12.5 nL 5 nL

1E-10 1E-09 1E-08 1E-07 1E-06 1E-05

Inhibitor Concentration (M)

% In

hib

itio

n

⊲ Pool and normalize samples, reagents, and primers by transferring different volumes from a collection of source plate wells

⊲ Cherry pick screen 'hits' in minutes for secondary screening

⊲ Quickly create combinations of samples and reagents in varying concentrations

⊲ Define transfer regions to create custom layouts

⊲ Compress libraries into interleaved patterns or separate quadrants

⊲ Offset transfers to any position in a well

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BROCHURE | ECHO® LIQUID HANDLERS FOR REGULATED LABORATORIES

Echo® Liquid Handlers Models

Cost-effective Plate Preparation and Superior Data Quality

Sample Management

Biologically Relevant Assays with Unmatched Data Quality

Cell-based Assays

Simplify Assay Workflows with Precise Reagent Transfers

Biochemical Assays

Enable Cost-Effective, Earlier Safety Screening

ADME-Tox

Maintain 21CFR11 Compliance While Using Echo® Liquid Handlers

Bioassays

Significantly Improving Workflows

Enabling Miniaturization with Unparalleled Throughput and AccuracyReagents, compounds, and samples used throughout the drug discovery process are transferred efficiently and accurately with Echo® Liquid Handlers.

With various throughput options and fluid transfer capabilities, you can use the Echo system from the early stages of drug discovery to drug

development and deployment.

Echo® 525RHigh Volume Transfer

Acoustic Liquid Handler

Echo® 550R SeriesFirst Generation

Acoustic Liquid Handler

Echo® 650R SeriesNext-Generation

Acoustic Liquid Handler

Transfer Volume: 25 nL – 5 μL Transfer Volume: 2.5 nL – 2 μL Transfer Volume: 2.5 nL – 2 μL

www.labcyte.com/applications

5

0.5 µLCells

10 µLCellTiter-Glo reagent

QS to 9 µLDirect Dilution

of TNF or Antibodyfor Dose Response on

media prefill

0.5 µLEC80 TNF challenge

or media

30-minuteIncubation

24-hourIncubation

Luminescent Readout

PHERAstar

384-well Low Volume Plate

LABCYTE INC.

Miniaturizing Bioassays with Acoustic Liquid Handling

Reduce Assay Volumes, Not Performance Successful miniaturization of bioassays requires the highly reproducible and accurate transfer of nanoliter and microliter volumes of cells, reagents,

and samples. Echo® Liquid Handlers deliver small volumes of these components accurately and precisely with flexibility and speed, enabling assay

miniaturization at previously unattainable levels without sacrificing quality. The 25 nL and 2.5 nL resolutions of Echo® Liquid Handlers allow for

direct dilution, whereby standard curves can be generated with an extended detection range and eliminating error-prone serial dilutions. In addition,

acoustic fluid transfers guard against cross-contamination due to the lack of any physical interaction with the liquid.

Host Cell Residual DNA Testing in Reduced Volume qPCR Reactions Traditional methods of quantitating residual host cell DNA are limited by laborious sample preparation protocols and lack of sensitivity. In addition,

recommended total reaction volumes are high to guard against the inaccuracy of human pipetting of sub-microliter reagent quantities. Assay total

reaction volumes can be reduced up to six-fold using Echo® Liquid Handlers, enabling for a large reduction in cost per reaction, additional replicates

and more confidence in the results generated.

The Echo® Liquid Handler was used

to reduce volumes of resDNASEQ™

Quantitative DNA Kits using human,

E. coli, and Chinese hamster ovary

(CHO) cell lines. In all cases, the

manufacturer recommended lower

limit of 0.03 pg in a 30 μL reaction

was capable of being lowered to

0.003 pg in a 5 μL reaction.

Representative qPCR traces of the

triplicate human, E. coli, and CHO

resDNASEQ results across multiple

orders of magnitude showing

reproducibility. Quantity of DNA per

reaction is annotated in picograms.

Human, E. coli, and CHO resDNASEQ

qPCR standard curves.

Miniaturized Humira TNF-Alpha L929 Cytotoxicity Bioassay For the Humira TNF-Alpha L929

Cytotoxicity Bioassay, the Echo® 525 Liquid

Handler enables a robust, miniaturized,

automated cell-based 384-well bioassay

workflow. The result is a 10 µL cell-based

assay performed with a single instrument,

which provides additional controls and

replicates in a miniaturized reaction to

reduce sample requirement, cost, and

potential human error.

Echo 655 Transferred resDNASEQ qPCR Kit40

30

20

Mea

n C q

pg DNA

10

0.001 0.01 0.1 1 10 100 1000 100000

Echo 655 Liquid Handler E. coli resDNASEQ34.175

31.175

28.175

25.175

22.175

19.175

16.175

13.175

10.175

7.175

4.175

1.175

10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40

Fluo

resc

ence

(465

–510

)

1.240

Echo 655 Liquid Handler Human resDNASEQ

36.240

32.740

29.240

25.740

22.240

18.740

15.240

11.740

8.240

4.740

10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40

Fluo

resc

ence

(465

–510

)

Echo 655 Liquid Handler CHO resDNASEQ

47.655

43.155

38.655

34.155

29.655

25.155

20.655

16.155

11.655

7.155

2.655

10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40

Fluo

resc

ence

(465

–510

)

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Echo® 21CFR11 Compliance Manager

BROCHURE | ECHO® LIQUID HANDLERS FOR REGULATED LABORATORIES

Echo® Software Applications

Quickly and Easily Develop Protocols for Echo® Liquid Handlers

Labcyte offers a full suite of Echo® Software Applications to enable researchers to quickly and easily create liquid handling protocols for specific

applications with minimal training. Each Echo application is designed around a specific liquid handling workflow and uses a combination of wizards

and graphical interfaces to simplify the creation of plate formats, liquid transfer routines, and output files. Researchers can quickly create a variety

of protocols off-line for Echo® Liquid Handlers and use built-in simulators to validate every transfer before running live. The suite of Echo software

applications enables Echo® Liquid Handlers to quickly and efficiently accomplish any liquid handling task.

Echo®

Array Maker

Echo®

Cherry Pick

Echo®

Combination Screen

Echo®

Dose- Response

Echo®

Plate Audit

Echo®

Plate Reformat

Echo®

Compliance Manager

www.labcyte.com/software

www.labcyte.com/ecm

Roles Guest User Power User Approver Admin

View APPROVED protocols and run reports

Run APPROVED protocols

Create, edit, and run DRAFT protocols

Edit APPROVED protocols

Override approval requests

Archive or delete project data

Disable OBSOLETE protocols from running

Manage and Track Users and Electronic Records

Echo® 21CFR11 Compliance Manager (ECM) locks down and tracks all Echo® Liquid Handler usage, protocol changes, and Echo file outputs using

state-of-the-art security algorithms. Using advanced checksum verification, the software detects any data tampering, assuring data integrity and

peace-of-mind. Compatibility with database management tools minimizes Information Technology infrastructure needs.

ECM offers five user access profiles to actively control and track:

• User access utilizing Microsoft Windows® Active Directory and AD-LDS

• Data organization including SQL database creation, backup, and restoration

• Protocol creation, changes, and approvals

• Echo run log file access and output

Seamlessly integrating with your favorite Echo® Liquid Handler applications, assay development and production

teams enjoy the full functionality of the Echo® Liquid Handler with the appropriate oversight necessary to meet

regulatory tracking and auditing requirements.

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Echo® Qualified Consumables

Service and Support

Maximize Your Instrument Performance

Echo® Qualified Consumables must meet the highest specifications to achieve the exceptional performance expected from Echo® Liquid Handlers.

Only consumables that are made of an acoustically compatible material and are exceptionally flat with extremely low inter- and intra-plate CVs are

considered qualified for use on Echo systems. Factory fluid calibrations developed specifically for Echo® Qualified Consumables support transfers

of a broad range of fluid types, providing maximum flexibility at the highest level of accuracy and precision.

Timely service and preventative maintenance are essential for optimal instrument performance and data quality. Labcyte offers a range of service

contract options to fit every lab’s needs and budget. Labcyte provides global field service support with local personnel in the United States, Europe,

and Asia. In addition to field support, we have support and instrument maintenance facilities in the United States, Europe, and Asia.

Labcyte offers superior application support dedicated to helping you achieve optimal results

from your Echo® Liquid Handlers and Access™ Automation Platforms. As a global organization

that serves customers in the United States, Europe, and Asia, Labcyte is a collaborative partner

that is committed to your success.

LABCYTE INC.

Precision Manufacturing and Exceptional Performance

⊲ 384-Well Polypropylene Microplates

⊲ 384-Well Low Dead Volume (LDV) Microplates

⊲ 1536-Well LDV Microplates

⊲ 384-Well Polypropylene Plus Microplates for aqueous transfer

⊲ 384-Well LDV Plus Microplates for aqueous transfer

⊲ 384-Well Tissue Culture Treated Polypropylene Microplates

⊲ 1536-Well Tissue Culture Treated LDV Microplates

⊲ 96-Tube acoustic sample tube racks (manufactured and sold by Brooks Life Sciences)

⊲ 6-Well Reservoirs

⊲ MicroClime® Environmental Lids

Global Applications Support Team

www.labcyte.com/consumables

www.labcyte.com/service

© 2019 LABCYTE INC. All rights reserved. Labcyte®, Echo®, MicroClime®, the Labcyte logo, Access™, andDynamic Fluid Analysis™ are registered trademarks or trademarks of Labcyte Inc., in the U.S. and/or other countries.

FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

LABCYTE INC.170 Rose Orchard WaySan Jose, CA 95134USA

Toll-free: +1 877 742−6548 | Fax: +1 408 747−2010

SALESNorth America | +1 408 747−2000 | info-us@labcyte.comEurope | +353 1 6791464 | info-europe@labcyte.comJapan | +81 03 5530 8964 | info-japan@labcyte.comAsia | +61 39018 5780 | info-us@labcyte.comOther | +1 408 747−2000 | info-us@labcyte.com

All product names and brands are properties of their respective owners.

BRH-ECRE-2.0MAR 2019