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Early L(1)sc expression: Are there L(1)sc equivalence groups?. Stage 9 8 L(1)sc + cells. Early Stage 10 All posterior cells L(1)sc + 9-10 L(1)sc + cells + MP3&4 Total of 11 -12 L(1)sc + cells. Stage 10: 2 glia underneath MP3 lighter L(1)sc expression 7 lsc+ cells around MP4 - PowerPoint PPT Presentation
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Early L(1)sc expression: Are there L(1)sc equivalence groups?
Stage 98 L(1)sc+ cells
Early Stage 10All posterior cells L(1)sc+
9-10 L(1)sc+ cells + MP3&4Total of 11 -12 L(1)sc+ cells
Stage 10: 2 glia underneath MP3lighter L(1)sc expression7 lsc+ cells around MP4Total of 11 l(1)sc+ cells
Lethal of Scute is Present in MPs and their Neuronal Progeny from Stages 9-11
L(1)Sc pdm2/nub merge
Stage 10
Stage 11
Stage 11
Stage 12
Present in PMG (s10-12)
Present in MNB
Present in all MPs (MP4, MP3, MP1, MP5, MP6) and newly divided neurons (H-cell, H-cell sib; mVUM5,6 and iVUM5,6)
Levels are asymmetric between H-cell and H-cell sib and between mVUM5 and iVUM5
l(1)sc may play a role in both neural precursor formation and establishing different daughter fates
Lethal of Scute is Present in MNB
L(1)sc wor
Reduced Numbers of Neurons are Present in AS-C Mutants
6 elav+ cells present from late 11-16Suggests that reduced numbers of neurons not due to cell death, but less neurons are generated
Stage 12 Stage 14 Stage 16W
ild -t
ype
Df(1
)scB5
7
AS-C Genes are Required for wor Expression
wor: MNBSuggests MNB does not form and does not produce its 8 progeny
wor merge
Wild
-typ
eD
f(1)s
cB57
CG8965 and wrapper are unaffected in AS-C mutants
wrapperCG8965
scB57: 5 CG8965+ cells/seg3.13 wrapper+ CG8965- cells/segn= 8 seg
High levels of wrapper did not always equal CG8965+ cells
wt: 3 PMG/seg @ s14 n=2
AS-C genes are Required for tbh Expression
4 5 6
mVUM
Tbh Cas TkriVUM
Cas(mVUM4,5; iVUM4,5)
Wild
-typ
eD
f(1)s
cB57
Tbh(mVUMs) merge
Tkr(mVUM6; iVUM6)
60% 50%
Suggest that mVUM6 and iVUM6 are present 60%
AS-C Phenotype : Precursor Formation and Neuron-Specific Control
MP1MP1 H-cell H-cellsib mVUM iVUM
AMG AMG AMG AMG
AMG MP3
PMG PMG
MNB
PMGPMG
MP4 MP5 MP6MP1AMG
L(1)sc
Notch
Numb
Neither Notch nor AS-C
Both L(1)sc and Notch
MNBp
Wild-type
AS-C Phenotype : Precursor Formation and Neuron-Specific Control
MP1MP1H-cell
sib iVUM
AMG AMG AMG AMG
AMG MP3
PMG PMG
MNB
PMGPMG
MP4 MP5 MP6MP1AMG
L(1)sc
Notch
Numb
Neither Notch nor AS-C
Both L(1)sc and Notch
AS-C mutant
X X
4 pdm2/nub+ Neurons are Present in AS-C mutants
pdm2/nub: MP1s, H-cell, H-cell sib
pdm2/nub
Stage 14
merge
Wild
-typ
eD
f(1)s
cB57
MP1MP1 H-cell H-cellsib mVUM iVUM
MP3 MP4 MP5 MP6MP1
AS-C Genes are Required for ple Expression
pdm2/nub: MP1s, H-cell & H-cell sib presentMP1 and H-cell sib gene expression unaffectedAS-C genes required for H-cell specific gene expression
Lim3 (MP1s)
CG13565 (H-cell sib)
ple (H-cell)
Wild
-typ
eD
f(1)s
cB57
H-cell H-cellsib
MP3MP1
MP1MP1
AS-C mutants
ac sc l(1)sc ase
D(f)sc 19X-ray derivative of In(1)ac3 pt mutation causes sc to lack C termSc protein nonfunctionalRearrangement that separates all 5’ seq of ac
Df(1)sc B57
ac sc l(1)sc aseX X
X X X X
X
Scute is Present in MPs, but not Neurons from Stages 10-11
Sc pdm2/nub merge
Stage 10
Stage 11
Stage 11
Stage 11
Present in PMG (s10-11)
Present in MP3, MP5, MP1, MP6, and MNB prior to division and is absent from newly divided neurons
May play a role in neural precursor formation, but not in establishing different daughter fates
Achaete Expression
Ac pdm2/nub merge
Stage 10
Stage 11
Stage 11
Stage 12
Present in MP1 and transiently in MP5, MP6, and MNB
Remains on in MP1 neurons to end of embryogenesis
May play a role in precursor formation and in MP1 neuron development