Dr.Arnolds Ubelis ([email protected]) The project: “DEVELOPMENT OF NANOTECHNOLOGY BASED BIOSENSORS FOR AGRICULTURE – BIOSENSORS-AGRICULT” PIRSES-GA-2012-318520

Dr.Arnolds Ubelis ([email protected] Ubelis ([email protected]).lv)

The project:The project:““DEVELOPMENT OF NANOTECHNOLOGY DEVELOPMENT OF NANOTECHNOLOGY BASED BIOSENSORS FOR AGRICULTURE BASED BIOSENSORS FOR AGRICULTURE

– BIOSENSORS-AGRICULT”– BIOSENSORS-AGRICULT”PIRSES-GA-2012-318520PIRSES-GA-2012-318520

DurationDuration 01.09. 01.09.2012-2012-31.08. 31.08. 20162016BudgetBudget 292,600.00292,600.00 Euro Euro

PEOPLEPEOPLE, , MARIE CURIE ACTIONSMARIE CURIE ACTIONS

Call: FP7-PEOPLE-2012-IRSES Call: FP7-PEOPLE-2012-IRSES International Research Staff International Research Staff

Exchange SchemeExchange Scheme


CoordinatorCoordinator

Dr.Arnolds UbelisDr.Arnolds [email protected]@latnet.lv

Association FOTONIKA-LVAssociation FOTONIKA-LVUniversity of LatviaUniversity of LatviaScientific secretary Scientific secretary

Dr.Roman ViterDr.Roman [email protected][email protected]

Technical assistant – Darta Ubele (Technical assistant – Darta Ubele (Darta Darta [email protected]@gmail.com) )


EU Project OfficerEU Project Officer

DAHON OLIVIERDAHON OLIVIEREuropean CommissionEuropean Commission

Research Executive AgencyResearch Executive AgencyUnit P2Unit P2

COV2 14/52COV2 14/52B-1049 Brussels/BelgiumB-1049 Brussels/Belgium

+32 2 296 4372 +32 2 296 4372 , , [email protected]@ec.europa.eu


ConsortiumConsortium

ParticipantParticipant Legal entityLegal entity DepartmentDepartment

1. Riga, 1. Riga, LatviaLatvia

University of Latvia,University of Latvia,LULU

Association FOTONIKA-LV,Association FOTONIKA-LV,((http://www.lu.lv/http://www.lu.lv/Fotonika-lvFotonika-lv//) )

2. Linköping, 2. Linköping, SwedenSweden

University of Linköping, University of Linköping, SwedenSweden

Department of Physics, Chemistry and Department of Physics, Chemistry and Biology(IFM) (Biology(IFM) (www.ifm.liu.sewww.ifm.liu.se ) )

3.3.Montpellier, Montpellier, FranceFrance

CNRS, Montpellier, CNRS, Montpellier, FranceFrance

European Institute of MembranesEuropean Institute of Membranes(www.iemm.univ-montp2.fr)(www.iemm.univ-montp2.fr)

4. Kyiv, 4. Kyiv, UkraineUkraine

National University of National University of Life and Environmental Life and Environmental SciencesSciences

Department of Molecular Biology, Department of Molecular Biology, Microbiology and BiosecurityMicrobiology and Biosecurity ((http://nubip.edu.ua/en/)http://nubip.edu.ua/en/)

5. 5. OdessaOdessa, , UkraineUkraine

Odessa National I.I. Odessa National I.I. Mechnikov University Mechnikov University

Faculty of Physics,Faculty of Physics, Faculty of Biology Faculty of Biology and Faculty of Chemistryand Faculty of Chemistry((www.onu.edu.uawww.onu.edu.ua,) ,)

6.Minsk, 6.Minsk, BelarusBelarus

National Academy of National Academy of Sciences of BelarusSciences of Belarus

Institute of Biophysics and Cell Institute of Biophysics and Cell Engineering of NASB, Engineering of NASB, (http://ibp.org.by/en/)(http://ibp.org.by/en/)


Exchange schemeExchange scheme

EU Non-EU

Staff exchange

Research teams will implement::-experimental work;-seminars, workshops;-summer school, conferences;-research training days.

Benefits and outcomes :-scientific publications;-knowledge transferre;-ideas for new projects;- new proposals.


Mutual Exchange program:

Scholars go abroad either as Experts (senior researchers) or as Trainees (young researchers) for a period of ca. three months;

Participating in research; Teaching or research training; Additional input to this IRSES research project, Master Thesis,

PhD thesis or similar projects on a biosensors activity, invited lectures;

Exchange balance: EU --- non-EU countries: in total ca. 53 PM spent abroad; Non –EU --- EU countries: in total ca. 101 PM spent in EU.

Time frame for staying abroad preferably: Minimum 1 month, maximum 12 months per researcher(more than 1 location possible)


Totally Totally for 4 project’s years - for 4 project’s years - 154 months/visits154 months/visits = 292 600 = 292 600 €€The first reporting period (1-24months) - The first reporting period (1-24months) - 9696 months/visits months/visits = 182 400 = 182 400 €€

The second reporting period (24-48months) - The second reporting period (24-48months) - 5858 months/visits months/visits = 110 200 = 110 200 €€

ParticipantParticipant Number of Number of months/ visitsmonths/ visits x x €€

1. Riga, Latvia1. Riga, Latvia 3030 x 1900 = 57 000 € x 1900 = 57 000 €

2. Linköping, Sweden2. Linköping, Sweden 1616 x 1900 = 30 400 € x 1900 = 30 400 €

3. 3. Montpellier, FranceMontpellier, France 77 x 1900 = 13 300 € x 1900 = 13 300 €

4. Kyiv, Ukraine4. Kyiv, Ukraine 3131 x 1900 = 58 900 € x 1900 = 58 900 €

5. 5. OdessaOdessa, Ukraine, Ukraine 3939 x 1900 = 74 100 x 1900 = 74 100 € €

6.Minsk, Belarus6.Minsk, Belarus 3131 x 1900 = 58 900 x 1900 = 58 900 € €


Table 2: List of Work Packages

Work package

n°

Work package titleInvolved teams

(lead team in bold)Start

month

End mont

h

1Design and investigations of

biosensitive layers

Riga, Montpellier, Linköping, Odessa,

Minsk, Kiev.1 20

2Technology development of

optical fiber and on chip sensor systems


Minsk, Kiev.12 27

3

Theoretical modelling of surface interaction between

biomolecules and biosensor surface


Minsk, Kiev.19 48

4

Sensitivity measurements of optical fiber and on-chip

laboratory made biosensors to different toxins


Minsk, Kiev.30 48


Summer Schools

The first summer school Location: Riga, Latvia Time: end of August 2013

The second summer school Location: Odessa, Ukraine Time: beginning of June 2014

The third summer school Location: Odessa, Ukraine Time: end of August 2016

Modules: (suggestions) Methods of academic research and writing for PhDs

“state-of-the-arts Theory of the scientific fields Experimental methods Data processing (error limits and systematic mistakes)


Conference ‘Biosensors for agriculture’

Responsibility ONU

Where Odessa, Ukraine

When August 2016

In cooperation with 3DOXIDES (FR), WU&Surfix (NL),

Ldiamon (ES), Sud-West photonics (GE)

Topic(s): Nanomaterials and Methods for detection of biomolecules

Participants besides project parnters

EU, USA and Asia

Audience Researchers, students, PhD students,

SMEs community


Timeframe, Milestones, Reporting: Interim report project months 24th;

Milestones:1) Proposal of algorithms for data management of

biosensing measurements. Draft report or draft of publication.

2) Insight and systematisation of state-of the-art knowledge on application of nanomaterials in biosensors

3) Survey of possibilities and elaboration of plans for field experiments to investigate processes on the surface of biosensitive layers.


Activities 2012Project months 1 to 4;

a) Planned – to start secondment visits in November;b) Delayed - due to readiness of consortia to susstain the

success by new complementary projects: - involvement of consortium members in writing and submition FP7 –PEOPLE-2013 –

ITN project proposal on 22.11.2012:

METONANOSENS’ Nr. ID 607534) - involvement of consortium members in writing and submision of FP7 –PEOPLE-2013 –IAPP project proposal on 16.01.2013: METOXNANOBIO Nr. Nr. ID612325

- overestimation of capacity to deal with formalities; - other reasons: annual reporting, exams and re-

asignement of contracts in non EU institutions.C) Activities of consortium performed in addition beyond

planned: see details in slides Nr. 29, 31, 34


Short referencies to the Scientific Content of the project in the next

slides


The The Project Project ConceptConcept

Biotechnology

Nanomaterials

Optical technology

Biosensors:Application in agriculture


Needs for agricultureNeeds for agriculture

-toxins, plant and animal diseases-toxins, plant and animal diseases -rapid express methods of diagnostics -rapid express methods of diagnostics -selectivity to one sort of biomolecules-selectivity to one sort of biomolecules -low cost of monitoring devices-low cost of monitoring devices

Biosensors – are the solutionBiosensors – are the solution


Biosensor definition (by Wikipedia)Biosensor definition (by Wikipedia)

A biosensor is an analytical device for the detection of an analyte A biosensor is an analytical device for the detection of an analyte that combines a biological component with a physicochemical that combines a biological component with a physicochemical detector that cannot detect.detector that cannot detect.

It consist of:It consist of: the sensitive biological element the sensitive biological element (biological material(biological material, , e.g. tissue, e.g. tissue,

microorganisms, organelles, cell receptors, enzymes, antybodies, nucleic asids, etc)microorganisms, organelles, cell receptors, enzymes, antybodies, nucleic asids, etc)

the transducer or the detector element the transducer or the detector element (works in a physicochemical way; (works in a physicochemical way;

optical, piezoelectric, electrochemical, etc.)optical, piezoelectric, electrochemical, etc.) that transforms the signal resulting that transforms the signal resulting from the interaction of the analyte with the biological element into from the interaction of the analyte with the biological element into another signal another signal (i.e., transducers)(i.e., transducers) that can be more easily measured and that can be more easily measured and quantified; quantified;

biosensor reader device with the associated electronics or signal biosensor reader device with the associated electronics or signal processors that are primarily responsible for the display of the processors that are primarily responsible for the display of the results in a user-friendly wayresults in a user-friendly way..


Advantages of Advantages of nanomaterialsnanomaterials

-low dimensions-low dimensions -quantum confinement effects-quantum confinement effects -high surface area-high surface area -affinity to bio molecules-affinity to bio molecules -many methods of depositions-many methods of depositions


Optical transducersOptical transducers

Indifferent to electromagnetic fieldsIndifferent to electromagnetic fields:: Stable in aggressive environmentStable in aggressive environment;; Precise measurementPrecise measurement;; Low cost and small size Low cost and small size (optical fibers)(optical fibers);;

Able to create network and distance Able to create network and distance sensingsensing..


Who will participateWho will participate

Experienced researchers Experienced researchers (professors, senior (professors, senior researchers, PhD holders with experience more than 4 researchers, PhD holders with experience more than 4 years)years);;

Early stage researchers Early stage researchers (experience less than (experience less than 4 years after obtaining MS degree)4 years after obtaining MS degree);;

Managers, engineers, programmers and Managers, engineers, programmers and technical stafftechnical staff


Implementation of the Implementation of the projectproject::

Linköping

KievOdessa Minsk

-Deposition of metal oxide nanostructures with CVP method; -Structural, optical and electrical characterization; -Bio fictionalization of nanostructures.


Riga

KievOdessaMinsk

-Characterization of optical fiber biosensors;-Characterization of biochips.




Montpellier

KievOdessaMinsk

-Deposition of metal oxide nanostructures with ALD method; -Structural, optical and electrical characterization;-Bio fictionalization of nanostructures .

Dr.Arnolds Ubelis ([email protected] Ubelis ([email protected]).lv)Dr.Arnolds Ubelis ([email protected])

Logo – Logo – main main and and additionadditional al modificmodifications ations ideasideas

Documents

Dr.Arnolds Ubelis ([email protected]) The project: “DEVELOPMENT OF NANOTECHNOLOGY BASED BIOSENSORS FOR AGRICULTURE – BIOSENSORS-AGRICULT” PIRSES-GA-2012-318520