January 15th 2015 Michael C. Golding - 1

Dr. Michael C. Golding, PhD

CONTACT INFORMATION Department of Veterinary Physiology Room 332 VMA College of Veterinary Medicine Texas A&M University College Station, Texas, USA 77843-4466 Office 979.862.1332 Fax 979.458.3635 mgolding@cvm.tamu.edu http://vetmed.tamu.edu/rsl/faculty/michael-golding

SUMMARY I am an assistant professor within the department of veterinary physiology and pharmacology at Texas A&M University. Here, I serve as the instructor of record for two courses studying human embryology and the physiological events of pregnancy. My research program focuses on understanding the biochemical mechanisms by which chromatin structure is altered during development and how these changes serve to regulate gene expression. I began my scientific career examining the role of failed epigenetic programming during the development of cloned embryos. I have since pioneered studies examining epigenetic mechanisms regulating imprinted gene expression and developmental programming within mammalian embryonic, extraembryonic and neural precursor stem cells. I am interested in understanding the mechanisms that recruit epigenetic modifiers to specific cohorts of genes, identifying the biochemical machinery directing this process, and understanding how these events are impacted by environmental toxins and teratogens. For the past four years, my laboratory has focused on epigenetic defects arising as a consequence of prenatal alcohol exposure. EDUCATION & TRAINING BSc in Honors Genetics, University of Western Ontario, London, Ontario Canada, 1996 – 2000 PhD in Veterinary Physiology, Texas A&M University, College Station, Texas USA, 2000 – 2003 Postdoctoral Fellowship, Cold Spring Harbor Laboratories, Cold Spring Harbour, New York USA,

2004 – 2006 Canadian Institute of Health Postdoctoral Fellow, University of Western Ontario, London, Ontario

Canada, 2006 – 2009 POSITIONS HELD June 2009 – Present Assistant Professor Department of Veterinary Physiology & Pharmacology Texas A&M University

January 15th 2015 Michael C. Golding - 2 AWARDS Texas A&M University Center for Teaching Excellence – 2013 – 2014 Service Learning Scholar. Zoetis 2013 Award for Research Excellence Texas A&M University Center for Teaching Excellence - Montague Scholar 2012 Texas A&M University - Richard H. Davis Teaching Award, 2012 Perinatal Research Society – Early Career Award, 2010 NIH Young Investigator Award, Perinatal Research Society, 2008 Wyeth Award for Excellence in Research, Department of Obstetrics and Gynaecology, 2007 Ontario Women’s Health Council/Canadian Institutes of Health Research - Institute of Gender

and Health, Postdoctoral Fellowship, 2006 Dr. David Whaley Postdoctoral Fellowship in Maternal/Fetal/Neonatal Research, 2006 TRAINEE AWARDS Kylee Veazey – 2013 Texas A&M College of Veterinary Medicine High Impact Achievement

Award – First Author Publication Kylee Veazey – 2012 Texas A&M College of Veterinary Medicine Outstanding Graduate Student

Scholarship Kylee Veazey – 2012 First Place Poster Presentation, Genetics Graduate Student Association

Mini-Symposium Kylee Veazey – 2011 Department of Genetics Regents Fellowship Kylee Veazey – 2010 Texas A&M College of Veterinary Medicine Graduate Research Trainee

Grant ADDITIONAL TRAINING Fall 2009 Texas A&M Center for Teaching Excellence – Teaching Development Series Infectious Disease and Blood Borne Pathogen Training, Environmental Health and Saftey, TAMU, 2009-2013 Biological Safety Training, BSL2, Office of Biosafety, TAMU, 2009-2013

January 15th 2015 Michael C. Golding - 3 RESEARCH PRIMARY RESEARCH PUBLICATIONS *My trainees are demarcated in red Golding MC, Ramsay M, Zhou FC, Redei EE, Charness ME. (2014) Epigenetic Insights into

Fetal Alcohol Spectrum Disorders. Alcohol Clinical and Experimental Research - Invited Review Submitted.

Suchodolski P, Golding MC (2014) The Noncoding RNA HOTAIR interacts with the mixed-

lineage leukemia 1 (MLL1) methyltransferase and promotes Hox gene activation during disease. Journal Biological Chemistry – Under Revision

Long CR, Westhusin ME, Golding MC. (2013) Reshaping the transcriptional frontier:

Epigenetics and somatic cell nuclear transfer. Molecular Reproduction and Development. Advanced Online Publication Oct 24 2013. PubMed PMID: 24167064.

Veazey KJ, Muller D. Golding MC (2013) Prenatal Alcohol Exposure and Cellular Differentiation

- A Role for Polycomb and Trithorax Group Proteins in FAS Phenotypes? – Alcohol Research: Current Reviews Volume 35 Issue 1. PubMed PMID: 24313167

Veazey KJ Carnahan MN, Muller D, Miranda RJ, Golding MC. (2013) Alcohol induced

epigenetic alterations to developmentally crucial genes regulating neural stemness and differentiation. Alcoholism: Clinical and Experimental Research 2013 Jul;37(7):1111-22. PubMed PMID: 23488822; PubMed Central PMCID: PMC3688681

Carnahan MN, Veazey KJ, Muller D, Tingling, JD, Miranda RJ, Golding MC (2013) Identification

of cell-specific patterns of reference gene stability in Quantitative Reverse Transcription Polymerase Chain Reaction studies of embryonic, placental and neural stem models of prenatal ethanol exposure. Alcohol Mar;47(2):109-20. PubMed ID: 23317542

Golding MC. (2012) Generation of trophoblast stem cells. Methods Molecular Biology. 925:49-

59. PubMed ID: 22907489 Tessanne K, Golding MC, Long CR, Peoples MD, Hannon G, Westhusin

ME. Production of transgenic calves expressing an shRNA targeting myostatin. Molecular Reproduction and Development. 2012 Mar;79(3):176-85. PubMed ID: 22139943

Article Featured on the March 2012 cover of Molecular Reproduction and

Development

January 15th 2015 Michael C. Golding - 4 Veazey KJ, Golding MC. Selection of stable reference genes for quantitative rt-PCR

comparisons of mouse embryonic and extra-embryonic stem cells. PLoS One. 2011;6(11):e27592. Epub 2011 Nov 10. PubMed PMID: 22102912; PubMed Central PMCID: PMC3213153

Golding MC, Magri LS, Zhang L, Lalone SA, Higgins MJ, Mann MR. Depletion of Kcnq1ot1 non-

coding RNA does not affect imprinting maintenance in stem cells. Development. 2011;138 3667-3678 PubMed ID PMID: 21775415

Golding MC, Williamson GL, Stroud TK, Westhusin ME, Long

CR. Examination of DNA methyltransferase expression in cloned embryos reveals an essential role for Dnmt1 in bovine development. Molecular Reproduction & Development. 2011 May;78(5):306-17. PMID: 21480430

Article Featured on the May 2011 cover of Molecular

Reproduction and Development Golding MC Mann MRW (2011) A Bidirectional Architecture Enhances Lentiviral Transgeneisis

in Embryonic and Extraembryonic Stem Cells. Gene Therapy. 2011 Mar 10. PubMed ID: 21390068.

Golding MC, Zhang L, Mann MR. Multiple epigenetic modifiers

induce aggressive viral extinction in extraembryonic stem cells. Cell Stem Cell 2010 6:1-11. PMID:20452320

Article Featured on the May 2010 cover of Cell Stem Cell Long CR, Tessanne KJ, Golding MC. (2010) Applications of RNA interference-based gene

silencing in animal agriculture. Reproduction Fertility & Development. 22(1):47-58. Silva JM, Marran K, Parker JS, Silva J, Golding M, Schlabach MR, Elledge SJ, Hannon GJ,

Chang K. Profiling essential genes in human mammary cells by multiplex RNAi screening. Science. 2008 319(5863):617-20.

ISSN 1040-452X

May 2011Volume 78, Issue 5

&Molecular Reproduction

Development

www.wileyonlinelibrary.com/journal/mrd

January 15th 2015 Michael C. Golding - 5 Kuzmin A, Han Z, Golding MC, Mann MR, Latham KE, Varmuza S. The PcG gene Sfmbt2 is

paternally expressed in extraembryonic tissues. Gene Expression Patterns. 2008 8(2):107-16.

Willingham-Rocky LA, Golding MC, Wright JM, Kraemer DC, Westhusin ME, Burghardt RC. Cloning of GJA1 (connexin43) and its expression in canine ovarian follicles

throughout the estrous cycle. Gene Expression Patterns. 2007 7(1-2):66-71 Golding MC, Long CR, Carmell MA, Hannon GJ, Westhusin ME. Suppression of prion protein

in livestock by RNA interference. Proceedings of the National Academy of Sciences U S A. 2006 4;103(14):5285-90.

Silva JM, Li MZ, Chang K, Ge W, Golding MC, Rickles RJ, Siolas D, Hu G, Paddison PJ, Schlabach MR, Sheth N, Bradshaw J, Burchard J, Kulkarni A, Cavet G,

Sachidanandam R, McCombie WR, Cleary MA, Elledge SJ, Hannon GJ. Second-generation shRNA libraries covering the mouse and human genomes. Nature Genetics. 2005 37(11):1281-8.

Harrington JR, Golding MC, Martens RJ, Halbert ND, Cohen ND. Evaluation of a real-time

quantitative polymerase chain reaction assay for detection and quantitation of virulent Rhodococcus equi. American Journal of Veterinary Research. 2005 66(5):755-61.

Golding MC, Westhusin ME. Analysis of DNA (cytosine 5) methyltransferase mRNA sequence

and expression in bovine preimplantation embryos, fetal and adult tissues. Gene Expression Patterns. 2003 3(5):551-8.

Jones DH, Golding MC, Barr KJ, Fong GH, Kidder GM. The mouse Na+-K+-ATPase gamma-

subunit gene (Fxyd2) encodes three developmentally regulated transcripts. Physiological Genomics. 2001 6(3):129-35.

BOOK CHAPTERS Golding Michael C. “Generation of Trophoblast Stem Cells.” Genomic Imprinting: Methods and

Protocols, Methods in Molecular Biology. Ed. Nora Engel. New York: Springer Science Media, vol. 925, 2012.

POST-PUBLICATION REVEIWS – Faculty of 1000 (www.F1000.com)

Michael Golding 27 Sept 2014 Reactivation of developmentally silenced globin genes by forced chromatin looping. Deng W,

Rupon JW, Krivega I, Breda L Gregory PD, Rivella S, Dean A, Blobel GA. Cell 2014 Aug 14; 158(4):849-60

Michael Golding 8 Aug 2014 Uniformity of nucleosome preservation pattern in Mammalian sperm and its connection to

repetitive DNA elements. Samans B, Yang Y, Krebs S, Sarode GV ... Wolf E, Steger K, Dansranjavin T, Schagdarsurengin U. Dev Cell 2014 Jul 14; 30(1):23-35

January 15th 2015 Michael C. Golding - 6 Michael Golding 14 Nov 2011 Retrotransposon-induced heterochromatin spreading in the mouse revealed by insertional

polymorphisms. Rebollo R, Karimi MM, Bilenky M, Gagnier L, ..., Jones S, Hirst M, Lorincz MC, Mager DL. PLoS Genet.2011 Sep; 7(9):e1002301

Michael Golding 14 Nov 2011 The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes. Gu TP, Guo F,

Yang H, Wu HP, ..., Szabó PE, Pfeifer GP, Li J, Xu GL. Nature. 2011 Sep 29;477(7366):606-10

Michael Golding 26 Oct 2011 lincRNAs act in the circuitry controlling pluripotency and differentiation. Guttman M, Donaghey

J, Carey BW, Garber M, ..., Regev A, Rinn JL, Root DE, Lander ES. Nature.2011 Sep 15; 477(7364):295-300

Michael Golding 26 Oct 2011 The noncoding RNA Mistral activates Hoxa6 and Hoxa7 expression and stem cell differentiation

by recruiting MLL1 to chromatin. Bertani S, Sauer S, Bolotin E, Sauer F. Mol Cell. 2011 Sep 16; 43(6):1040-6

Michael Golding 24 May 2011 Endogenous retroviruses and neighboring genes are coordinately repressed by LSD1/KDM1A.

Macfarlan TS, Gifford WD, Agarwal S, Driscoll S, ..., Franco L, Rosenfeld MG, Ren B, Pfaff SL. Genes Dev. 2011 Mar 15; 25(6):594-607

Michael Golding 02 June 2011 Aberrant over-expression of satellite repeats in pancreatic and other epithelial cancers. Ting DT, Lipson D, Paul S, Brannigan BW, ..., Rivera MN, Bardeesy N, Maheswaran S, Haber

DA.Science. 2011 Feb 4; 331(6017):593-6 INVITED TALKS January 7, 2015, A Cross-Disciplinary Service-Learning Project Serving The Brazos Valley

Autism Clinic. TAMU College of Veterinary Medicine – Teaching Showcase. College Station Texas, USA.

October 28th 2014 Epigenetic Programming and Fetal Alcohol Syndrome. Department of

Neuroscience and Experimental Therapeutics – Departmental Seminar Series. College Station Texas USA.

October 17th 2014 Epigenetic Programming and Fetal Alcohol Syndrome. Interdisciplinary

Faculty of Reproductive Biologists – Annual R.O. Berry Research Retreat. Navasota Texas USA.

April 25th 2014 Service-Learning at Texas A&M – Learning opportunities within the Brazos Valley

Autism Clinic. TAMU Center for Teaching Excellence Seminar Series. College Station Texas, USA.

April 21st 2014 Teratogens and Epigenetic Programming. Texas A&M Interdisciplinary Faculty of

Toxicology – Research Seminar. College Station Texas USA.

January 15th 2015 Michael C. Golding - 7 April 12th 2012 An epigenetic basis to fetal alcohol spectrum disorders. Texas A&M Genetics

Graduate Student Association - Research Symposium. College Station Texas, USA. June 27th 2011 The role of transposable elements in lineage-specific developmental programming

and the epigenetic impact of in utero alcohol exposure. Epigenetic Insights into Fetal Alcohol Spectrum Disorders Symposia – Research Society on Alcoholism Annual Meeting, Atlanta Georgia, USA.

September 25th 2010 Epigenetic Control of Gene Expression in the Embryonic and

Extraembryonic Lineages. Early Career Speaker – Perinatal Research Society Annual Meeting, Avon, Colorado, USA.

May 13th 2009 Quantum of Silence: Screening for Modifiers of Retroviral Silencing in the

embryonic and extraembryonic lineages using RNA interference. Obstetrics and Gynaecology Research Day, Department of Obstetrics and Gynaecology, St Joseph’s Hospital, London, Ontario, Canada.

May 12th 2009 Quantum of Silence: Screening for Modifiers of Retroviral Silencing in the

embryonic and extraembryonic lineages using RNA interference. Department of Molecular and Cellular Biology Roswell Park Cancer Institute, Buffalo, New York USA.

March 6th 2009 Screening for modifiers of retroviral silencing in embryonic and extraembryonic

stem cells using RNA interference. Great Lakes Mammalian Development Meeting, University of Toronto Conference Center, Toronto, Ontario, Canada.

November 19th 2008 Quantum of Silence: Screening for Modifiers of Retroviral Silencing in the

embryonic and extraembryonic lineages using RNA interference. Department of Physiology, Texas A&M University, College Station, Texas, USA.

October 2008 RNA Interference. Prenatal Research Group, St Josephs Hospital, London,

Ontario, Canada. May 14th 2008 Screening for Modifiers of Genomic Imprinting Using RNA Interference.

Obstetrics and Gynaecology Research Day, Department of Obstetrics and Gynaecology, St Joseph’s Hospital, London, Ontario, Canada.

May 2nd 2007 Screening for Modifiers of Genomic Imprinting Using RNA Interference. Obstetrics

and Gynaecology Research Day, Department of Obstetrics and Gynaecology, St Joseph’s Hospital, London, Ontario, Canada.

March 8th 2007 Evade and Escape: A Viral System for Stable Delivery of RNAi into Embryonic

and Placental Stem Cells. Cancer Research Laboratory Program Seminar Series, University of Western Ontario, London, Ontario, Canada.

November 17th 2006 RNA Interference: Biology to Technology – The New Mammalian Genetics.

Department of Systems and Cell Biology, University of Toronto, Toronto, Ontario, Canada.

January 15th 2015 Michael C. Golding - 8 October 31st 2006 Challenges Facing Postdoctoral Fellows at Canadian Universities. Canadian

Institute of Health Research – Institute of Human Development and Youth Health – Research Forum, University of Western Ontario, London, Ontario, Canada.

October 13th 2006 RNA Interference: Biology to Technology – The New Mammalian Genetics.

Texas Reproductive Biology Forum, Texas A&M University, College Station, Texas, USA. June 8th 2006 RNA Interference: Biology to Technology – The New Mammalian Genetics.

Experimental Oncology Seminar Series, London Health Sciences Center, University of Western Ontario, Victoria Hospital, London, Ontario, Canada.

December 6th 2005 Large Scale Identification of Molecular Elements Involved in the Maintenance

of Imprinted Gene Silencing by an shRNA library Screen. Laboratory of Cancer and Developmental Biology, National Cancer Institute, Fort Detrick, Frederick, Maryland, USA.

January 14th 2004 Analysis of DNA (cytosine 5) methyltransferase mRNA sequence and

expression in cloned and IVF bovine Preimplantation embryos. Department of Animal Sciences, University of Guelph, Guelph, Ontario, Canada.

POSTER PRESENTATIONS & ABSTRACTS *My trainees are demarcated in red Snyder MD, Pryor JH, Veazey KJ, Peoples MD, Williamson GL, Golding MC, Westhusin ME,

Long CR. Suppression of SETDB1 During Bovine Embryonic Development Results in Preimplantation Mortality and Decreased Transcription of Transposable Elements Congress on Animal Reproduction, Reno Nevada, January 2015

Veazey KJ, Skiles WM, Miranda RC, Parnell S, Golding, MC. An Epigenetic Basis to the Etiology

of Fetal Alcohol Spectrum Disorders. Epigenetics Canada Conference. London, Ontario, June 24-27 2014.

Ramos Y, Jaggi C, Benson N, Lozano K, Muller D, Golding MC. The Impact of Polychlorinated

Biphenyls on Sox2 Transcription. Texas A&M Student Research Week, March 2014, College Station Texas, USA.

Hoenig M, Muller D, Golding MC. The Effects of Bisphenol A on Sox2 Regulated Neural

Differentiation. Texas A&M Student Research Week, March 2014, College Station Texas, USA.

Burra B. Muller D. Golding M.C. The Consequences of Diethylstilbestrol Exposure on the Sox2

Gene Expression. Texas A&M Student Research Week, March 2014, College Station Texas, USA.

January 15th 2015 Michael C. Golding - 9 Synder M.D*., Pryor, J.H., Peoples, M.D., Williamson, G., Golding, M.C., Westhusin, M.E. and

Long, C.R. Suppression of Epigenetic Modifiers Alters the Bovine Embryonic Modifiers Alters the Bovine Embryonic Developmental Program During In Vitro Culture. International Congress on Animal Reproduction, Reno Nevada, January 2014.

* Student Competition Presentation Burroughs CA*, Williamson, G.L., Golding M.C. Long CR. Oxidative stress induced changes in

epigenetic modifying gene mRNA in pre-implantation in vitro bovine embryos. . International Congress on Animal Reproduction Reprod Dom Animals Sept 2013. • Student Competition Presentation

Wenegast D, Veazey KJ, Golding MC. Antioxidants and Fetal Alcohol Spectrum Disorders: An epigenetic link? Merial-NIH Veterinary Scholars Symposium. East Lansing Michigan, August 2013.

Crocker A. Muller D. Golding M.C. Effects of Alcohol on the Regulation of Imprinted Genes in

Mouse Stem Cells. Texas A&M Student Research Week, May 2013, College Station Texas, USA.

Villenouva E. Muller D. Golding M.C. Effects of Alcohol on the Regulation of Imprinted Genes in

Mouse Placental Stem Cells. Texas A&M Student Research Week, May 2013, College Station Texas, USA.

Burroughs C., Wilkerson A.P., Golding M.C., Long C.R. TET gene family transcript profiles

during bovine embryo pre-implantation stages. 17th International Congress on Animal Reproduction. Vancouver, British Columbia, Canada, January 2013

Muller D., Carnahan M.N., Golding M.C. Analysis of imprinted genes in ethanol-exposed murine

stem cell lineages. Texas A&M Genetics Graduate Student Association - Research Symposium. April 2012 College Station Texas, USA.

C.A. Burroughs, A.J. Pearks-Wilkerson, M.C. Golding and C.R. Long (2012) TET gene family

expression profiles during bovine embryo preimplantation stages. International Congress on Animal Reproduction Reprod Dom Animals 47(Suppl.4) 1702.

M. Peoples, M.C. Golding, C. Long and M. Westhusin (2012) Development of rLentiviral vectors

for male germline-specific Cre recombinase expression in livestock. Reproduction, Fertility and Development 24:183.

Veazey K.J., Carnahan M.N., Miranda R.J., Golding M.C. Enthanol-induced errors in the

epigenetic regulation of developmentally crucial genes in neural stem cells. Texas A&M Genetics Graduate Student Association - Research Symposium. April 2012 College Station Texas, USA.

G.L. Williamson, J.H. Pryor, K. Tessanne, M.C. Golding and C.R. Long (2011) Suppression of

Suz12 in bovine preimplantation embryos via cytoplasmic siRNA injection. Reproduction, Fertility & Development

January 15th 2015 Michael C. Golding - 10 M. Peoples, M. Westhusin, M.C. Golding and C. Long (2010) Characterization of lentiviral short-

hairpin RNA expression vectors containing single or multiple bovine polymerase III promoters. Texas Forum on Reproductive Sciences, Houston, Texas.

K. Tessanne, M.C. Golding, C. Long, and M. Westhusin (2009) Suppression of bovine and

caprine myostatin expression in vitro using RNA interference. Reproduction, Fertility & Development 21(1): 251.

Peoples MD*, Sadeghieh S, Hwang E, Chen S, Hannon G, Golding MC, Long CR, Westhusin M

(2009) Inhibition of Foot and Mouth Disease Virus in vitro using RNA interference. Reproduction, Fertility & Development 21(1): 103.

* Student Competition Presentation Linger GD, Bormann CL, Peoples MD, Golding MC and Long CR (2009) Expression Profiling of

Histone Modifying Genes During Bovine Preimplantation Embryo Development In Vitro. Keystone Symposium Proceedings: Chromatin Dynamcs and Higher Order Organization, Coeur d’Alene, Idaho.

Linger GD, Bormann CL, Peoples MD, Golding MC and Long CR (2009) Expression Profiling of

Histone Modifying Genes in Bovine Preimplantation Embryos. Reproduction, Fertility and Development 21(1): 193.

K. Tessanne, C. Long, M.C. Golding, and M. Westhusin. 2009. Comparison of different methods

for producing transgenic livestock with reduced myostatin expression using RNA interference. Texas Forum on Reproductive Sciences, Houston, Texas.

Golding M.C. Screening for modifiers of retroviral extinction in embryonic and extraembryonic

stem cells. IFRB Retreat 2009 – Texas A&M University College Station Texas USA. Golding M.C. Screening for modifiers of retroviral extinction in embryonic and extraembryonic

stem cells. Gordon Research Conference Mammalian Gametogenesis & Embryogenesis, 2009 Waterville Valley, New Hampshire USA.

Lalone, S.A., Golding, M.C., McWilliam, M., Kaufman, L. Higgins, M.J. and Mann. M.R.W.

Characterization of the Mouse Kcnq1ot1 Non-coding RNA. SSR July 18-22, 2009, in Pittsburgh, Pennsylvania USA.

Lalone, S.A., Golding, M.C., McWilliam, M., Kaufman, Higgins M.J. and Mann M.R.W.,

Characterization of the Kcnq1ot1 Noncoding RNA and Its Role in Imprinted Gene Regulation during Preimplantation Development, Obstetrics and Gynaecology Research Day, May 13th 2009, London Ontario Canada.

Lalone, S.A., Golding, M.C., McWilliam, M., Kaufman, L. Higgins, M.J. and Mann. M.R.W.

Characterization of the Mouse Kcnq1ot1 Non-coding RNA. Great Lakes Mammalian Development Meeting, University of Toronto Conference Center, Toronto, Ontario, Canada.

Magri L.S., Golding, M.C. and Mann M.R. Screening for epigenetic factors controlling genomic

imprinting during early mammalian development. Margret Moffat Research Day, March 26th 2009, London, Ontario, Canada.

January 15th 2015 Michael C. Golding - 11 Linger GD, Bormann CL, Peoples MD, Golding MC and Long CR (2008) Expression Profiling of

Histone Modifying Genes in Bovine Preimplantation Embryos. Reproduction, Fertility and Development 20: 168.

Lalone, S.A., Golding, M.C., McWilliam, M., Kaufman, L., Louriero, K., Mann M.R.W.,

Characterization of the Kcnq1ot1 Noncoding RNA and Its Role in Imprinted Gene Regulation during Preimplantation Development, Obstetrics and Gynaecology Research Day, May 14th 2008, London Ontario Canada.

Foster, J.E.G., Golding, M.C, Perrier, N., and Mann, M.R.W., Identification and Characterization

of Novel Imprinting Centers, Obstetrics and Gynaecology Research Day, May 14th 2008, London Ontario Canada.

Market B.A. Golding M.C. Mann M.R.W., Molecular and developmental effects of various culture

regimes in a mouse model system, Obstetrics and Gynaecology Research Day, May 14th 2008, London Ontario Canada.

Linger, G.D., Bormann, C.L., Peoples, M., Golding M.C. and Long, C.R., Expression Profiling of

Histone Modifying Genes in Bovine Pre-Implantation Embryos. Internation Embryo Transfer Society (IETS) January 5th – 9th 2008, Denver, Colorado, USA.

Varmuza S, Kuzmin A, Latham K, Han Z, Mann M, and Golding M, The PcG Gene Sfmbt2 is

Maternally Imprinted in Extraembryonic Tissues, Keystone Symposium on Epigenetics: Regulation of Chromatin Structure in Development and Disease, April 11-16, 2007, Breckenridge, Colorado, USA.

Varmuza S, Kuzmin A, Latham K, Han Z, Mann M, and Golding M, The PcG Gene Sfmbt2 is

Maternally Imprinted in Extraembryonic Tissues, Keystone Symposium on Epigenetics: Regulation of Chromatin Structure in Development and Disease, April 11-16, 2007, Breckenridge, Colorado, USA.

Varmuza S, Kuzmin A, Chow J, Wen S, Latham K, Han Z, Mann M, and Golding M, The PcG

Gene Sfmbt2 is Maternally Imprinted in Extraembryonic Tissues, Great Lakes Mammalian Development Meeting, March 1-2, 2007, Toronto, Ontario Canada.

Golding M.C., Paddison P.J., Silva J.M. and Hannon G.J., Development of a Plasmid Based

Expression System for the Production of mir30 Based shRNAs. Keystone Symposia on Diverse Roles of RNA in Gene Regulation, January 8-14, 2008, Breckenridge, Colorado USA.

Golding M.C., Long C.R., Shin, T., and Westhusin M.E., Small Hairpin Mediated Disruption of

Bovine DNMTs in Nuclear Transfer and Parthenogenic Embryos. LXIX Cold Spring Harbor Quantitative Symposia on Quantitative Biology – Epigenetics, June 2-7 2004, Cold Spring Harbor, New York, USA.

Golding M.C., Shin, T., Kreamer, D. and Westhusin M.E., Sequence and Expression Analysis of

the DNA Methyltransferase (DNMT) Family in Normal Bovine and Nuclear Transfer Preimplantation Development. Keystone Symposia on Chromatin and Epigenetics, February 21 - 26, 2002, Taos, New Mexico USA.

January 15th 2015 Michael C. Golding - 12 Jones, D.H., Golding, M.C. and Kidder, G.M., Expression of Sodium Pump g Subunit Isoforms In

Preimplantation Mouse Development. Gordon Research Conference on Mammalian Gametogenesis and Embryogenesis, Jul 1-6 2000, New London, Connecticut USA..

January 15th 2015 Michael C. Golding - 13 MEETING PARTICIPATION AND ATTENDANCE (2002-PRESENT) Epigenetics Eh! The Canadian Congress on Epigenetic Research. Trainee delivered a short talk.

London Ontario Canada, June 24-27 2014. Research Society on Alcoholism – Epigenetic Insights into Fetal Alcohol Spectrum Disorders

Symposia – Invited Speaker, Atlanta Georgia, July 2010. Perinatal Research Society Annual Meeting, Avon Colorado USA, Early Career Speaker,

September 2009. Gordon Research Conference Mammalian Gametogenesis & Embryogenesis, Waterville Valley

New Hampshire, USA, Poster presentation July 2009. Meeting of the Society for the Study of Reproduction, 2009 Pittsburg Pennsylvania USA. Session

Chair – July 2009. Great Lakes Mammalian Development Meeting, Toronto, Ontario, Canada,– Speaker. February

2009 Great Lakes Mammalian Development Meeting, Toronto, Ontario, Canada,– Participant. February

2008 Great Lakes Mammalian Development Meeting, Toronto, Ontario, Canada,– Poster. February

2007 Gordon Research Conference Mammalian Gametogenesis & Embryogenesis, 2006 New London,

Connecticut, USA, Participant. Great Lakes Mammalian Development Meeting 2006 Toronto, Ontario, Canada,– Participant. Keystone Conference – Diverse Role of RNA in Gene Regulation, 2005, Breckinridge Colorado,

USA,– Poster. Cold Spring Harbor Symposia on Quantitative Biology – Epigenetics, 2004 , Cold Spring Harbor,

New York, USA, Poster. Keystone Conference on Chromatin, 2003, Keystone, Colorado, USA, Participant. Keystone Conference on Chromatin and Epigenetics, 2002, Taos New Mexico, USA,– Poster.

January 15th 2015 Michael C. Golding - 14 COMMERCIAL DEVELOPMENT SBI – Systems Bioscience - Bidirectional Promoter Lentiviral Vectors www.systembio.com

In 2011, I published a novel vector architecture that enhanced transgene stability and expression within embryonic and extra-embryonic stem cells. Systems Biosciences, a biotechnology company based in California, approached me during the summer of 2012 looking to develop this novel architecture into a commercial reagent. Working through Blake Petty at the Texas A&M University System, we negotiated the commercialization of this product as well as its implementation in other viral expression systems. In 2013, these products became commercially available and my laboratory continues to enjoy a very lucrative relationship with SBI. https://www.systembio.com/lentiviral-technology/expression-vectors/cdna/vector-maps-bidirectional

January 15th 2015 Michael C. Golding - 15 GRANTS AND CONTRACTS AWARDED Grants and Contracts Summary

Grants and Contracts Details A. Pending Research Support March of Dimes - $225,000.00 July 2015 to June 2017

Title: A role for male inherited epigenetic abnormalities in the etiology of fetal alcohol syndrome.

Principle Investigator: Michael C Golding B. Active Research Support NIH National Institute of Alcoholism and Alcohol Abuse $379,235.00 1R21AA022484 (September 2014 to August 2016) Title: Altered genomic imprinting as a basis for FASD placental growth defects

Principle Investigator: Michael C Golding

Texas A&M University Center for Teaching Excellence $5000.00 2013 – 2014 Service Learning Scholarship Program. (Fall 2013 to Fall 2014) Principle Investigator: Michael C. Golding Texas A&M University Center for Teaching Excellence $6500.00 2012 Montague Scholarship (Fall 2012 – Fall 2015) Principle Investigator: Michael C. Golding

Types and Role Total $s to all PIs External Competitive PI $518,057 Co-PI $2,949,950 Subtotal $3,468,007 Internal PI $11,500 Co-PI $31,441 Subtotal $46,441 Other Gifts and Gifts-in-Kind 0 Royalties to Program 0 Subtotal 0 Total $3,514,448

January 15th 2015 Michael C. Golding - 16 NIH National Center for Research Resources - $1,131,200 1R24RR032683-01 (September 2011 to August 2015) Title: Inducible tissue specific transgene expression in large animal biomedical models Principle Investigator: Charles R. Long Co-investigator: Michael C. Golding

Texas Agrilife - $31,441 (Summer 2011 to August 2014)

Title: Effect of maternal nutrient restriction on fetal hepatic development and long-term metabolic outcomes Principal Investigator: M. Carey Satterfield Co-Principal Investigator: Michael C. Golding

NIH- National Institute of Child Health and Human Development - $1,818,750 1R01HD058969-01A2 (April 2010 to March 2015) Title: Functional analysis of the embryonic epigenome in a non-rodent model Co-Principal Investigators: Mark E. Westhusin, Michael Golding, Charles R. Long (equal contributions)

C. Completed Research Support NIH National Institute of Alcoholism and Alcohol Abuse $138,822 1RO3AA020129-01 (September 2010 to August 2013) Title: Measuring the impact of prenatal alcohol exposure on the fetal epigenome

Principle Investigator: Michael C Golding Texas A&M College of Veterinary Medicine $5,000 Graduate Student Trainee Award 2011 (one year). An epigenetic basis to fetal alcohol spectrum disorders.

Principle Investigator: Kylee Veazey Supervisor: Michael C Golding

Texas A&M College of Veterinary Medicine $10,000 Postdoctoral Trainee Award 2011 (one year). Retroviral Transgenesis.

Principle Investigator: Paulette Suchodolski Supervisor: Michael C Golding

NIH- Ruth L. Kirschstein Postdoctoral Fellowship $160,000 Title: Minorities and Women in Science and Engineering Primary Supervisor: Michael C. Golding

January 15th 2015 Michael C. Golding - 17

GRANTS SUBMITTED DATE AGENCY MECHANISM PROPOSAL TITLE PI STATUS

September 2014

March of Dimes

Research Grant

A role for male inherited epigenetic abnormalities in the etiology of fetal alcohol syndrome

Michael Golding

Pending Review

January- 2014 NIH RO1

Impact of Alcohol Exposure on the Male Epigenetic Developmental Program

Michael Golding

Not Discussed

November-2013 NIH R21

Altered genomic imprinting as a basis for FASD placental growth defects - Resubmission

Michael Golding Awarded

October-2013 NIH RO3

Epigenetic Strategies to Enhance Innate Antiviral Immune Responses

Neetu Singh

Not Discussed

September-2013 NIH RO1

Functional Analysis of Epigenetic Programming Using CRISPR/Cas Gene Editing

Mark Westhusin

Not Discussed

October-2012 NIH R21

Altered genomic imprinting as a basis for FASD placental growth defects

Michael Golding

Scored, Not Funded

October-2012 NIH RO1

An epigenetic basis to alcohol-induced central nervous system patterning defects

Michael Golding

Not Discussed

October-2012 NIH RO1

The epigenetic origins of fetal alcohol syndrome - Resubmission

Michael Golding

Scored, Not Funded

October-2012 NIH RO1

Environment induced epigenetic effects on mammalian preimplantation development

Charles Long

Scored, Not Funded

October-2012 NIH R24

Development of Large Animal Models for Human Disease and Biomedical Research

Mark Westhusin

Scored, Not Funded

September-2012

March of Dimes

Research Grant An epigenetic basis to alcohol induced birth defects

Michael Golding Not Funded

September-2012 NIH RO1

Gene Signature for Spontaneous Regression in Melanoma

Duane Kraemer

Scored, Not Funded

December-2011 NHARP

Research Grant The Epigenetic Origins of Fetal Alcohol Syndrome

Michael Golding Not Funded

October-2011 NIH RO1 The epigenetic origins of fetal alcohol syndrome

Michael Golding

Scored, Not Funded

October-2011

NIH / USDA RO1

Molecular signatures of disease arising from compromised pregnancy

Carry Satterfield

Scored, Not Funded

October-2011 NIH RO1

Environment induced epigenetic effects on mammalian preimplantation development

Charles Long

Scored, Not Funded

January 15th 2015 Michael C. Golding - 18 September-

2011 March of Dimes

Research Grant

The epigenetic origin of alcohol induced neurological birth defects

Michael Golding Not Funded

February-2011 NIH R21

Alcohol-induced alterations in chromatin remodeling during neuronal development

Michael Golding

Scored, Not Funded

February-2011 NIH R21

Resistance to transgene silencing mediated by a divergent promoter architecture

Michael Golding

Not Discussed

November-2010 NIH RO1

Inducible Tissue-Specific Transgene Expression in Large Animal Biomedical Models

Charles Long Awarded

September-2010

March of Dimes Grant

An epigenetic basis to alcohol induced central nervous system birth defects

Michael Golding Not Funded

June-2010 NIH R21 Dietary Phytochemicals and Epigenetic Programins in Metastic Cancer

Michael Golding

Not Discussed

June-2010 NIH RO1 Epigenetic Silencing of Retroviruses as a Model of Tumor Suppressor Gene Silencin

Michael Golding

Not Discussed

June-2010 Searle Scholar

Research Grant The role of noncoding RNAs in programming cell fate.

Michael Golding Not Funded

February-2010 NIH RO3

Measuring the impact of prenatal alcohol exposure on the fetal epigenome

Michael Golding Awarded

January-2010 USDA

Program Grant

Engineering disease resistance to vesicular stomatitis virus in livestock using RNA interference.

Michael Golding

Scored, Not Funded

November-2009 NIH R21

Epigenetic control of endogenous retro-elements in the early embryonic lineages

Michael Golding

Scored, Not Funded

October 2009 NIH RO1

Functional analysis of the embryonic epigenome in a non-rodent model

Mark Westhusin Awarded

September-2009 NHARP

Research Grant Retrotransposons as Vectors for Gene Therapy.

Michael Golding Not Funded

January 15th 2015 Michael C. Golding - 19 TEACHING COURSES TAUGHT Fetal and Embryo Physiology - VTPP 452 Spring Semester 2012 – Present Instructor of Record – Michael C. Golding (Previously VTPP 489) 3 Credit Hours

This is a course I developed during the 2011 academic year, the purpose of which is to provide undergraduate students with an introduction to the physiological processes essential to gametogenesis, early embryonic development and pregnancy. This course will provide a framework upon which to build a basic understanding of the physiology of pregnancy and several developmental disorders that arise due to biomedical miss-regulation or environmental agents. Students are expected to synthesize structural functional relationships between the gametes and reproductive organs and describe the interaction between the developing fetus, placental membranes, maternal tissues and the environment. This course will cover essential elements underpinning the molecular basis of development and disease, which will be expanded upon in both medical and veterinary professional programs as well as relevant graduate courses.

Fetal and Embryo Physiology – VTPP 652 Spring Semester 2012 – Present Instructor of Record – Michael C. Golding (Previously VTPP 689) 3 Credit Hours

This is a stacked graduate course taught in conjunction with VTPP 452 – Fetal and Embryo Physiology. This covers the same materials but requires an expanded writing assignment and participation in a weekly journal club.

One Health in Action – BIMS 110 Fall Semester 2014 - Present Instructor of Record – Michael C. Golding 1 Credit hour

This is a course I developed during the 2014 academic year, which is intended to provide incoming freshman Biomedical Sciences students an appreciation of how separate areas of medicine, biology, ecology and economics overlap with each other. This course follows a survey model and seeks to expand the student’s breadth of knowledge regarding the connections between epidemiology, biotechnology, virology, environmental toxicology, climate science, animal ethics and genetics. Grading for this class is derived from weekly quizzes administered through ecampus.

January 15th 2015 Michael C. Golding - 20 Epigenetics and Systems Physiology – VTPP 651 Summer Semester 2011 - Present Instructor of Record – Michael C. Golding 3 Credit hours

The purpose of this course is to provide graduate level students with a journal club style seminar class focusing on the most current research in Epigenetics as it relates to developmental biology and disease. Students will be assigned two weekly papers taken from the most current primary literature. Each student will be expected to synthesize the data and research strategies outlined in each paper. For each manuscript discussed, one student will be expected to give an oral presentation detailing the research findings while another student will be assigned to lead the discussion of the paper. Each week, two papers will be discussed and marks will be assigned based on the quality of the oral presentations and active group participation. Marks for this class will be assigned as either pass or fail.

Physiology - VTPP 605 Fall Semester 2011 – Present Instructor of Record – Christine Heapes. 5 Credit Hours

VTPP 605 and 606 are core courses offered by the Department of Veterinary Physiology and Pharmacology, designed to provide a basic understanding of mammalian physiology to non-Physiology majors as an essential element in advanced graduate studies. This course covers diverse aspects of cellular physiology including the physiology of excitable membranes, body fluids, neurophysiology, cardiovascular physiology, skeletal muscle, endocrinology, reproductive biology, gastrointestinal function and covers many of the molecular mechanisms underlying these processes. I provide lectures on molecular cell biology, mechanisms of cell signaling, mechanisms of hormonal control, reproductive endocrinology, reproductive physiology and gamete biology.

Physiology - VTPP 606 Spring Semester 2011 – Present Instructor of Record - Christine Heapes. 5 Credit Hours

VTPP 605 and 606 are core courses offered by the Department of Veterinary Physiology and Pharmacology, designed to provide a basic understanding of mammalian physiology to non-Physiology majors as an essential element in advanced graduate studies. This course covers diverse aspects of cellular physiology including the physiology of excitable membranes, body fluids, neurophysiology, cardiovascular physiology, skeletal muscle, endocrinology, reproductive biology, gastrointestinal function and covers many of the molecular mechanisms underlying these processes. I provide lectures on mammalian preimplantation development, placentation, organogenesis and fetal pathophysiology.

January 15th 2015 Michael C. Golding - 21 Gamete and Embryo Physiology - VTPP 659 Spring Semester 2010 - Present Instructor of Record - Charles R. Long 3 Credit Hours

VTPP 659 is an elective course designed to provide an advanced understanding of the Physiology of mammalian gametogenesis, preimplantation mammalian development and reproductive endocrinology and function in both livestock and laboratory animals. I provide lectures covering topics on genetic modification, stem cell-based therapies, epigenetics and the impact of environmental teratogens on early development.

Vascular Physiology - VTPP 655 Fall Semester 2012 - Present Instructor of Record - Christine Heapes 4 Credit Hours

VTPP 650 is an elective, journal club style class designed to examine multiple aspects of vascular physiology including: the structure and function of blood vessels, vascular beds, the molecular and cell biology of endothelium and vascular smooth muscle, microcirculation, regulation of blood flow by local, neural and humoral signals. In this course, I teach two lectures focusing on vascular aspects of placentation and maternal-fetal blood flow.

Texas A&M Center for Teaching Excellence – Service Learning Community Service Project

Co-Directors Dr. Michael Golding – BIMS program Dr. Mandy Rispoli – Special Education Program Service-learning is a form of experiential education in which students engage in activities that address human and local community needs. Students enrolled in VTPP 452/652 - Fetal and Embryo Physiology, are given the opportunity to volunteer within the local autism clinic and collaborate with Special Education majors from SEFB 414. Together, groups of students work under the direction of Dr. Amy Heath to fulfill a need of the autism clinic (client interactions, producing learning materials ect). This project seeks to build a cross-disciplinary experience for students enrolled within the Biomedical Sciences and Educational Psychology programs at Texas A&M University. This service-learning opportunity will prompt students to step outside their area of comfort and allow them to develop both novel perspectives as well as critical thinking skills that would otherwise not be available in either program individually.

January 15th 2015 Michael C. Golding - 22 STEM Teaching Professional Development Course Faculty Mentor - Michael Golding 2015 Graduate Student Mentee – Kristin Hicks 2014 Graduate Student Mentee – Daria Muller

The STEM Teaching Professional Development Course prepares PhD students to teach science, technology, engineering, and mathematics courses within the undergraduate curriculum. Students enrolled in the course engage in active-learning techniques, such as written exercises, collaborative learning groups, and discussions to enhance their understanding of the theory and practice of effective instruction. At the end of the semester, students are given the opportunity to design and teach a course module within their faculty mentor’s undergraduate class. I enroll my graduate students in this course and require them to give a lecture in my course – VTPP 452 / 652 Fetal and Embryo Physiology.

GUEST LECTURES PHEB 612 Reproductive and Perinatal Epidemiology – Developmental Programming and

Epigenetics. (October 8 2014). SEFB 414 Methods and Issues in Low-Incidence Disabilities – 2 units: 1) Basic Principles of

Development 2) Developmental Origins of Birth Defects. Fall 2013 - Present BIMS 320 / GENE 320 – Biomedical Genetics, 1 unit - Epigenetics and the fetal origins of adult

disease. 2012 - Present VTPP 489 / 689 – Stem Cell Biology, 1 unit – Epigenetic Programming and Differentiation, Spring

Fall 2012 – Present (offered every two years) VTPP 667 – Molecular Pharmacology, 1 unit – Epigenetic Gene Regulation, Spring Term 2010 –

Present

January 15th 2015 Michael C. Golding - 23 LABORATORY TEACHING DeBakey Institute Research and Leadership Programs

This undergraduate focused program seeks to implement novel strategies to create leadership and research opportunities for students with little or no laboratory experience. Students are organized into work groups with a senior mentor student and several novice students. The mentor student is charged with both learning the laboratory techniques and then passing these skills on to the other members of their group. I provide the project and train the senior student, who then passes the skill sets along to the freshman and sophomore level trainees. Dr. Quick (VTPP) initiated this program in 2014. STUDENT NAME YEAR POSITION PROJECT TITLE

Yanell Ramos 2013 Leader Cloning the Sox2 Promoter Chandni Jaggi 2013 Mentee Cloning the Sox2 Promoter Kristin Lozano 2013 Mentee Cloning the Sox2 Promoter Nahessi Benson 2013 Mentee Cloning the Sox2 Promoter

Veterinary Medical Scientist Research Training Program (VMSRTP) Program Leaders: Dr. Roger Smith and Dr. Charles R. Long

This program is intended to introduce veterinary professional students to research and promote relationships between research scientists and veterinarians. During the summer semester, first and second year veterinary students with an interest in research are paired with faculty mentors and given a research project. Students are expected to complete a defined project, correlate the data and present their findings at both an in house seminar as well as at a national meeting. Since 2012 I have co-supervised 2 students and mentored one.

STUDENT NAME YEAR MY ROLE CURRENT POSITION Daniel Wenegast 2013 Mentor Second Year Vet School Andrew Garcia 2013 Co-Supervise with Dr. Long Second Year Vet School Raena Furtado 2012 Co-Supervise with Dr. Long Third Year Vet School

January 15th 2015 Michael C. Golding - 24 UNDERGRADUATE STUDENTS TRAINED

STUDENT NAME YEAR MAJOR PROJECT TITLE Jessica Mondshine 2014 BIMS Screening for Activators of Thyroid Katherine Elston 2014 BIMS Screening for Activators of Thyroid Bhaskari Burra 2014 BIMS Environmental Toxins & Thyroid Sig Megan Hoenig 2014 BIMS Environmental Toxins & Thyroid Sig Yanell Ramos 2013 BIMS Cloning the Sox2 Promoter Chandni Jaggi 2013 BIMS Cloning the Sox2 Promoter Bhaskari Burra 2013 BIMS Cloning the Sox2 Promoter Kristin Lozano 2013 WFSC Cloning the Sox2 Promoter Nahessi Benson 2013 BIMS Cloning the Sox2 Promoter Elizabeth Villaneuv 2012 ANSC Genomic Imprinting in the Placenta Alyssa Crocker 2012 ANSC Genomic Imprinting in the Embryo Victoria Giles 2011 BIMS Genomic Imprinting

GRADUATE STUDENTS TRAINED Thesis Graduate Students

STUDENT NAME YEAR ENROLLED

DEPARTMENT MY ROLE

Sarah Chronister 2014 VTPP Chair Haiqing wang 2013 VTPP Member Richard Cheng-an Chang 2013 VTPP Member Mikaela Gondolfe 2013 ANSC Member Srikanth Kanameni 2013 VTPP Member Mathew Snyder 2013 VTPP Member William Skiles 2012 VTPP Co-Chair Ashley Keith 2012 ANSC Member Daria Muller 2012 VTPP Chair Jessica Cardin 2012 KINE Member Ali Abdelnabi 2011 POUSC Member Kylee Veazey 2010 GENE Chair

January 15th 2015 Michael C. Golding - 25 BIMS Non-Thesis Masters Students

STUDENT NAME YEAR ENROLLED MY ROLE Megan Willoughby 2014 Member Michael Overby 2014 Member Taelor Pastine 2014 Member Maisie Llewellyn 2014 Chair Miguel Carpio 2014 Member Kaili (Tony) Zhang 2014 Chair Angela Bein 2014 Member Edwin Mcelroy 2014 Member Carson Doty 2014 Member Brandon Poskevich 2014 Chair Krytsal Green 2014 Member Hannah Crawford 2014 Member Armando Mondragon 2013 Member Borna Sarkar 2013 Member Lauren Carnes 2013 Chair Wendy Carazo 2013 Chair Ashley Ghazizadeh 2013 Member

January 15th 2015 Michael C. Golding - 26 STUDENTS GRADUATED

STUDENT NAME Degree Year My Role Dept CURRENT WHEREABOUTS Lindsay Miller Msc NT 2014 Member BIMS Applying to Vet School Sarah Kim Msc NT 2014 Member BIMS PA School Ashley Thomas Msc NT 2014 Member BIMS Medical School Texas A&M Cameron Jacobs Msc NT 2014 Member BIMS Vet School – Oklahoma St. U Daniel Cortez Msc NT 2014 Member BIMS Dental School – Houston Gina Jammal Msc NT 2014 Member BIMS Medical School – Texas A&M Amber Kendrick Msc NT 2014 Chair BIMS Vet School – Texas A&M Laura Suratt Msc NT 2014 Member BIMS Applying to Vet School Onkar Sawant PhD 2013 Member VTPP Postdoc – Cleveland Clinic Joy Barros Msc NT 2013 Chair BIMS UAB – Health Education Pgm Christina Wilcox Msc 2013 Member STJR Working as Scientific Writer Heather Johnson Msc NT 2013 Member BIMS Applying to Med School Michael Peoples Msc 2012 Member VTPP Embryo Tech. MD Anderson Sarah Stiehl Msc NT 2012 Member BIMS Manager Tyler Chicken Ashley Navarrette Msc NT 2012 Chair BIMS Vet School – Texas A&M Andrew Nagar Msc NT 2012 Member BIMS Applying to Med School Michael Uzelac Msc 2012 Member ANSC Sales rep for Zoetis Gregory Burns Msc 2012 Member VTPP PhD Program U Washington Lizbeth Remirez Msc 2011 Member VTPP PhD Program Texas A&M Jessica Elliot-Miles

Msc 2011 Member ANSC Technician Firey Ranch OK

Yue Shi Msc 2011 Chair GENE Med School U of Chicago NT – Non Thesis option STUDENTS WITHDRAWN

STUDENT NAME DEGREE MY ROLE

DEPT CURRENT WHEREABOUTS

Kyle Bautsch Msc NT Member VIBS Accepted to Dental School Sarah Wiggins Msc NT Chair BIMS Accepted to PA School Samantha Campbell Msc NT Chair VTPP Accepted to TAMU Vet School

STUDENTS SUPERVISED Kim Tessanne, PhD Student, Department of Veterinary Physiology & Pharmacology.

Applications of RNAi to large animal biotechnology. June 2009-Dec 2010. Gayle Wiliamson, MSc. Student, Genetics Program Texas A&M University. Examination of

epigenetic modifiers in bovine embryos. June 2009-Present Lauren Magri, PhD Student, Department of Biochemistry, UWO. Screening for epigenetic

modifiers of genomic imprinting using RNAi, January 2009 – June 2009. Julia Foster, PhD Student, Department of Biochemistry, UWO. Isolation of imprinting centers,

June 2007 – June 2009.

January 15th 2015 Michael C. Golding - 27 Brenna Market, MD/PhD Student, Department of Biochemistry, UWO. Molecular and

developmental effects of various culture regimes in a mouse model system, August 2007 – June 2009

Morgan McWilliam, Volunteer and Summer Student, Department of Biology, UWO. Cloning the 5’ end of the noncoding transcript Kcnq1ot1. April 2007 – September 2008.

Michelle Gabriel, Volunteer and Summer Student, Department of Biology, UWO. Cloning the shRNA library into a new lentiviral vector, January - September 2007.

Sarah Lalone, MSc Student, Department of Physiology, UWO. Characterization of the non-coding RNA Kcnq1ot1 in early mouse development, January 2007 – June 2009.

Nicole Perrier, 4th Year Honors Student, Department of Biochemistry, UWO. Isolation of imprinting centers, September 2006 – May 2007.

Liana Kaufman, 4th Year Honors Student, Department of Biochemistry, UWO. Cloning the 3’ end of the noncoding transcript Kcnq1ot1, June 2006 – May 2007.

Sihem Cheloufi, PhD Student, Cold Spring Harbor Laboratories. Role of argonaute proteins in early mammalian development, November 2004 – September 2005.

Suzanne Menges, PhD Student, Animal Science, Texas A&M University. Disruption of epigenetic modifications associated with somatic cell nuclear transfer, September 2002 – February 2004.

Melisa Cox, PhD Student, Department of Genetics, Texas A&M University. Cloning of the K9 collagen cDNA, September 2002 – December 2003.

Carole Hanna , PhD Student, Animal Science, Texas A&M University. Application of RNAi to studies of bovine preimplantation development, September 2002 – February 2004.

Lauri Willingham, PhD Student, Anatomy and Public Health, Texas A&M University. Cloning of Gja1 (connexin43) and its expression in canine ovarian follicles throughout the estrous cycle, March 2002 – February 2004.

January 15th 2015 Michael C. Golding - 28 SERVICE UNIVERSITY REVIEWER

Internal Reviewer – USDA Formula Animal Health proposals 2012, 2013 College of Veterinary medicine and Biomedical Sciences Postdoctoral and Graduate

Fellowships - Reviewer Fall 2011, 2012.

COMMITTEE MEMBERSHIP

College of Veterinary Medicine and Biomedical Sciences - Non-Thesis Masters Steering Committee – 2014 to Present

Department of Veterinary Physiology & Pharmacology Search Committee – Teaching

Faculty Position for VTPP 423 January 2013 to Present. College of Veterinary Medicine and Biomedical Sciences - Advanced Developmental

Training Initiative Scholarship Committee – Summer 2011 - Present

Medical Sciences Library Council – Member Fall 2011 to Present College of Veterinary Medicine and Biomedical Sciences – Bush School Travel

Scholarship Committee 2010 – to Present

College of Veterinary Medicine and Biomedical Sciences Merit Scholar Committee – Founding Member - June 2011 to Present

College of Veterinary Medicine and Biomedical Sciences Graduate Recruitment

Committee – Member Fall 2011 to 2012 Texas A&M Interdisciplinary Faculty of Reproductive Biologists – Nominating Committee

2010 to present. Reproductive Sciences Complex Steering Committee (2009- present)

Children’s Health Research Institute Task Force for Excellence (Member) 2007 – 2009 Children’s Health Research Institute Trainee Leadership Committee (Chair) 2007 - 2009

January 15th 2015 Michael C. Golding - 29 PROFESSIONAL REVIEWER

National Institute of Health – Institute of Environmental Health Sciences (NIH-NIEHS) – Reviewer for ZES1 Scientific Review Group - Child Health and the Environment Review Committee. May 12th to 14th 2015.

National Institute of Health – National Institute on Alcohol Abuse and Alcoholism

(NIH-NIAAA) – Tertiary Reviewer, AA1 Study Section on research into fetal alcoholism. March 3rd 2015.

Lalor Foundation – 2015 External Reviewer for Postdoctoral Fellowships Genome Canada – Reviewer for the Genome Canada College of Reviewers Scientific

Review Group - Large-Scale Applied Research Project Competition: Genomics and Feeding the Future. December 2014

Society for the Study of Reproduction - 2014 Abstract Reviewer, Embryology Section.

National Institute of Health – Institute of Environmental Health Sciences (NIH-

NIEHS) – Reviewer for ZES1 Scientific Review Group – Programs in Superfunds and Related Sites. October 2013

National Institute of Health – Institute of Environmental Health Sciences (NIH-

NIEHS) – Reviewer for ZES1 Scientific Review Group - Children's Health and the Environment. November 2012

National Institute of Health – National Institute on Alcohol Abuse and Alcoholism

(NIH-NIAAA) – Tertiary Reviewer, AA1 Study Section on research into fetal alcoholism. October 2011

January 15th 2015 Michael C. Golding - 30 CONTINUING EDUCATION INSTRUCTOR Veterinary Information Network - Continuing Education Online Course - GENETICS, EPIGENETICS AND STEM CELLS INSTRUCTORS: Jennifer G. Barrett, DVM, Ph.D., Diplomate ACVS - Virginia Tech University Jerold S. Bell, DVM – Tufts University Kathleen Cavanagh, Honors B.Sc., DVM, MET – University of Guelph, Canada Michael C. Golding, B.Sc., Ph.D. – Texas A&M University REAL TIME SESSIONS: May 16, 2013; April 17th 2015. 8:00-10:00 pm ET (US)

This online course is designed for veterinarians interested in learning more about genetics, stem cell biology and epigenetics as applied to everyday veterinary practice. The scope of the course is to introduce the basic underlying principles of these rapidly evolving disciplines to practicing veterinarians so that they become more familiar with their potential clinical application. I team teach this short course once a year in conjunction with faculty from University of Guelph in Canada as well as Tufts University, Virginia Tech, and Wake Forest University here in the United States. This course is RACE approved for veterinarians and counts as 8 continuing education credits as mandated by state licensing boards.

EDITORIAL SERVICE - Reviewer Alcoholism, Clinical and Experimental Research (Impact Factor 3.4) Biology of Reproduction (Impact Factor 4.0) BMC Developmental Biology (Impact Factor 2.7) Cellular Reprogramming (Impact Factor 2.7) Development (Impact Factor 6.2) Developmental Dynamics (Impact Factor 2.6) Epigenetics (Impact Factor 4.5) Epigenomics (Impact Factor 2.4) Genome Medicine (Impact factor 4.94) Genome Research (Impact Factor 11.3) Genomics (Impact Factor 3.0) International Journal of Molecular Sciences (Impact Factor 2.6) Journal of Animal Science (Impact Factor 1) Journal of Biotechnology (Impact Factor 3.3) Molecular Biotechnology (Impact Factor 2.2) Molecular Reproduction and Development (Impact Factor 2.8) PLOSOne (Impact Factor 3.7) Reproduction, Fertility and Development (Impact Factor 2.5) Theriogenology (Impact Factor 2.5)

January 15th 2015 Michael C. Golding - 31 CONSULTANTSHIPS

Revolution Whitetails, Scientist, 2004 - 2009 Allerca Transgenics, Scientist, 2003 – 2006

PROFESSIONAL AFFILIATIONS

Interdisciplinary Faculty of Reproductive Biologists, Texas A&M 2009 - Present Interdisciplinary Faculty of Toxicology, Texas A&M 2014 - Present

Society for the Study of Reproduction – 2009 -Present Perinatal Research Society 2010 - Present

Faculty of 1000 Member 2010 – Present American Physiological Society, 2004 – 2006

COMMUNITY SERVICE

July 16th 2013 – Texas 4H Frontiers in Science Research Tour

Lead a tour and information session discussing the research activities / mission of the reproductive sciences complex for a statewide 4H meeting.

Change Bandit, Children’s Health Foundation, 2007-2009 Fundraiser for Canadian National Service Dogs, 2006 – 2009