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Dr Amélie Barthès, EUROFINS Hydrobiologie France Julien Blaise, EUROFINS Expertises Environnementales Environmental Impact Survey in offshore project: focus on marine hydrobiology methodologies application 2018 (21st, september) RemTech Expo 2018 (19, 20, 21 Settembre) FerraraFiere www.remtechexpo.com

Dr Amélie Barthès, EUROFINS Hydrobiologie France Julien

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Dr Amélie Barthès, EUROFINS Hydrobiologie FranceJulien Blaise, EUROFINS Expertises Environnementales

Environmental Impact Survey in offshore project: focus on marine hydrobiology methodologies application

2018 (21st, september)

RemTech Expo 2018 (19, 20, 21 Settembre) FerraraFierewww.remtechexpo.com

I. Presentation of Eurofins HydrobiologieFrance

→Presentation of Eurofins network→ Team of European specialists→Our references in offshore/coastal projects

II. Environmental surveys in offshore / coastal projects

→Our offer →Personalized services→ International framework and Accreditations→ Focus on ecotoxicity testing (Julien Blaise)

SUMMARY

Since 30 years, Eurofins Scientific is a major actor in environmentalanalyses in the world with:

• Over 38 000 employees• 400 laboratories• 44 countries• Over 150 000 analytical methods

Fields of activities of Eurofins Expertises Environnementales:

→ Ecotoxicology

→Radioactivity

→ Specialized microbiology

→Hydrobiology :

Eurofins Hydrobiologie France

EUROFINS Network

EUROFINS Hydrobiologie France

▪ Various aquatic ecosystems

Running water : torrents, rivers, streams, …

Stagnant water : lakes, canals, gravel pits…

Marine waters

Brackish waters : lagoons, ponds, …

▪ Different biological and physicochemical compartments

Microalgae, aquatic plants, plankton, macroinvertebrates, fishes,

water and sediments sampling, …

WHAT IS HYDROBIOLOGY?

Science which studies on aquatic biological communities (flora and fauna)

-Impact studies and monitoring of

ecological state (WFD)

Team of european specialists

Eurofins: European team for widespread capabilities !

➢ Main european collaborators

▪ The Netherlands – Hydrobiology in marine and

freshwater environments / Ecotoxicology

→ 16 specialists in 2 sites

▪ France – Hydrobiology in marine and freshwater environments

→ 15 specialists in 4 sites

- Ecotoxicology : 6 specialists in 1 site

➢ But also Germany, Finland, Denmark, Ireland, Norway and Sweden

Main references in offshore/coastal projects

➢ North Sea, Mediterranean Sea, Manche Sea

▪ Benthic fauna, dredging,

▪ Sediment and water analyses,

▪ ROV video monitoring

➢ Africa

▪ Sudan : Sampling and analyses of benthos, water and sediments

▪ Cameroun : Sampling management for the characterization of initial state of a naturalgas project

▪ Congo : Health risk monitoring of fishes in 3 lagoons

➢ In order to support our partners and customers

Sudan – Sampling mission…

… before analyses in our

laboratories

I’m Lisa !

Our offer in offshore/coastal projects

▪ Eurofins Hydrobiologie France is your unique partner for supporting your

environmental monitoring of marine coastal or offshore projects:

→ Feasibility studies for Renewable energy projects (wind power, solar farms, electric

connections…)

→ Exploration and construction work

→ Operational phase

→ Maintenance work

▪With adapted equipments: laboratory dedicated to marine biology analyses, SIG mapping,

communication media, spatial and temporal comparisons, statistical analyses...

▪ Personalized services for the development, the monitoring and the maintenance

of coastal or offshore engineering projects:

→ Adjustment sampling plans, protocols and analysis

→ Field sampling support and achievement

→ Logistics

→ High quality analysis

→ Interpretation of results: raw data, expertise, surveying and reviewing

Our offer in offshore/coastal projects

Personalized services

▪ Field sampling, protocols and analyses

Marine sediments

Marine water sampling

On site measurements

→ Grab sampler or professional diving

→ Niskin bottle

→ Multi parameter probe

→ Physicochemical analyses

Personalized services

▪ Professional diving

→ Inventories of marine biodiversity

→ Monitoring of structures colonisation (reef effect)

→ Fauna and flora monitoring: protected species (Posidonia meadow, Pinna sp.,…)

→ Immersion of mussels samples (bioindicator)

Personalized services

▪ Benthos monitoring

➢ Standard : NF EN ISO 16665 (March, 2014)

➢ European requirements : Water Framework Directive

→ Ecological state assessment and monitoring (WFD)

→ Use of indexes : AMBI, M-AMBI, REBENT and other diversity indexes

▪ Phytoplankton monitoring

➢ Standard : NF EN 15972 (December, 2011)

➢ European requirements : Water Framework Directive

→ Quantitative and qualitative studies

→ Species determination

Personalized services

→ Determine and assess the state of the aquatic environment

→ Assess the impact of human activities on aquatic ecosystems

→ Complementary with ecotoxicological/ physical and chemical parameters

Food quantity and quality

Benthic macrofauna

Important link in trophic webs

Reflect environmental conditions

Relative immobilityLifetime= few years

StreamsGrain Size distributionWaters quality

Personalized services

How works a soft bottom Benthic Macrofauna study ?

Step 2: Laboratory

analyses

Step 1: Field sampling

Step 3: Results, interpretationand discussion

International framework

▪ Our studies answer to regulation laws in France, Europe, …

▪ Protocols are adapted to:

WFD territorial waters (DCE-DCSMM) and Exclusive Economic Zone

Mining Code of the International Seabed Authority

RINBIO (french network on biological integrators) protocols

REBENT (benthic network) protocols for European requirements

Customer specifications….

▪ For each analysis→ ISO is applied (International standard)

WWW.COFRAC.FR

→ Benchmark ISO/CEI 17025 – COFRAC accreditation (France)

Ecotoxicity tests on freshwater

Surface on the water

- Water lens (Lemna minor)

Water column

- Daphnia (Daphnia magna)

- Freshwater algal (Raphidocelis

Subcapitata)

- Ceriodaphnies (Ceriodaphnia dubia)

- Rotifers (Brachionus calicyflorus)

Sediment

- Chironomes (Chironomus riparius)

- Ostracods (Heterocypris incongruens)

Ecotoxicity tests on marine water

Marine copepod (Arcatia tonsa)

Amphipod (Corophium arenarium)

Crustaceans (Artemia salina)

Bivalves (Crassostrea gigas)

Echinoides (Paracentrotus lividus)

Bacteria (Vibrio fischeri)

Internal method

«Coral cuttings toxicity test (Seriatopora hystrix)»

Why use this Organization?

- Observation of coral bleaching in the oceans

- Breeding sites for many species

- Very important for marine ecosystems

▪ Coral cuttings brought into contact in each concentration of the sample

▪ Duration of the test: 96h (renewal of the medium at 48h)

▪ Biological descriptors: NOEC and LOEC

→ Observation of whitening and retraction of polyps

Waste and sediment management

HP 14 Criterion

▪ INDIRECT Approach

▪ DIRECT Approach

Analyzes realized on the ELUATE from the sampleleaching (aquatic ecotoxicity)ACUTE TOXICITY and CHRONIC TOXICITY

Acute toxicity analyzes realized on the RAW SAMPLE (Ground ecotoxicity)

The positive answer of a single test is enough to classify the waste or the sediment as ecotoxic for the environment of the basis of thresholds

established in the regulation.

GRAZIE PER L’ATTENZIONE,

Dr. Amélie Barthès / Julien Blaise

EUROFINS Hydrobiologie France

+33 6 37 47 57 71 / +33 6 80 32 18 25

[email protected] / [email protected]