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Table of Contents

Monday .............................................................................................................. 4

Tuesday ............................................................................................................. 5

Wednesday .................................................................................................... 8

Thursday .......................................................................................................... 11

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Monday ............................................................................................................. 16

Tuesday ............................................................................................................ 19

Thursday .......................................................................................................... 21

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Wednesday ................................................................................................... 23

ScheduleOverview Water Microbiology Conference 2015

At the University of North Carolina at Chapel Hill

May 18 - 21

Registration Opens, Breakfast Available

Side Events -

Break - Atrium

Side Events -

Lunch - Trillium Dining Room

Side Events -

Break - Atrium

Side Events -

7:30 AM

8:30 AM

10:00 AM

10:30 AM

Noon

1:00 PM

2:30 PM

3:00 PM


Side Events -

Break - Atrium

Side Events - Redbud,


Keynote Address - Dr. Ronald Atlas (Grumman Auditorium)

Break - Atrium

Verbal Presentations


Poster Reception - Atrium

Director’s Reception - by Invitation

7:30 AM

8:30 AM

10:00 AM

10:30 AM

Noon

1:00 PM

2:15 PM

2:45 PM

4:00 PM

5:00 PM

5:30 PM

Thursday 5/21


Side Events Verbal Presentations - Dogwood

Keynote Address - Dr. Marylynn Yates (Grumman Auditorium)

Side Events Verbal Presentations


Side Events - Dogwood

Break - Atrium

Side Events - Dogwood

7:30 AM

8:30 AM

9:45 AM

11:00 AM

Noon

1:00 PM

2:30 PM

3:00 PM

ScheduleOverview



Keynote Address - Dr. Gary Toranzos (Grumman Auditorium)





Break - Atrium

Keynote Address - Dr. Huw Taylor (Grumman Auditorium)

Poster Reception - Atrium

Conference Dinner - Trillium Dining Room

7:30 AM

8:30 AM

9:45 AM

11:00 AM

Noon

1:00 PM

2:15 PM

3:15 PM

3:45 PM

5:00 PM

6:30 PM

8:30 - 10:00 AM Side Events

10:00 - 10:30 AM Break

10:30 - Noon Side Events

Noon - 1:00 PM Lunch - Trillium Dining Room

1:00 - 2:30 PM Side Events

DetailedSchedule

Convened by University of North Carolina at Chapel Hill's Institute of Marine Sciences and the Department of Environmental Sciences and Engineering

REDBUD

Climate Change and Waterborne Diseases

Convened by the University of Connecticut and Water Institute at UNC

BELLFLOWER

Convened by University of North Carolina at Chapel Hill's Institute of Marine Sciences and the Department of Environmental Sciences and Engineering

REDBUD

REDBUD (continued)

Climate Change and Waterborne Diseases

BELLFLOWER (continued)

5

DetailedSchedule1:00 - 2:30 PM Side Events

2:30 - 3:00 PM Break


(cont.)

Training

Convened by Artel

WINDFLOWER

Convened by Georgia State University

BELLFLOWER

REDBUD (continued)

BELLFLOWER (continued)

8:30 - 10:00 AM Side Events

Disinfection of Reuse Water, Wastewater and Biosolids -

Convened by WEF Disinfection and Public Health Committee

REDBUD

Pathogenic Environmental Mycobacteria: Detection, Sources,

Convened by US Environmental Protection Agency

BELLFLOWER

10:00 - 10:30 AM Break

10:30 - Noon Side Events

Noon - 1:00 PM Lunch

1:00 - 2:15 PM Keynote Address - Grummand Auditorium

“One Health” - Dr. Ronald Atlas

2:15 - 2:45 PM Break

2:45 - 3:45 PM Verbal Presentations

(cont.)

and Training

Convened by Artel

WINDFLOWER

How to Test, Teach and Treat Water in Rural Areas of Developing

Countries to Eliminate Waterborne Disease

Convened by International Water and Health Alliances

DOGWOOD

Water Reuse

Should Water Treatment Planning for Potable Reuse Directly Address Norovirus Risk? Presented by: Jeffrey Soller, Soller Environmental LLC

Microbial Quality of Reclaimed Water to Meet NC Type 2 Performance for Escherichiacoli, Coliphage Viruses, Salmonella spp., and Clostridium perfringensEmily Bailey, The University of North Carolina at Chapel Hill

Effective use of Peracetic Acid to reduce effluent disinfection byproduct in Water Resource Recovery FacilitiesPresented by: Isaiah Shapiro, Greeley and Hansen

REDBUD

DetailedSchedule

6


5:00 - 6:30 PM Poster Reception - Atrium

5:30 PM Director’s Reception - By Invitation

REDBUD

A Comparison of EPA Method B for Bacteroidales to Traditional Fecal Indicator BacteriaPresented by: Curtis Stumpf, Crystal Diagnostics

Assessment of the Microbial Community in the Siem Reap River, Siem Reap, Cambodia Presented by: Jennifer Mendell, Bridgewater State University

Concentration of Microorganisms by Concentrating Pipette and Wet Foam ElutionPresented by: Andrew Page, InnovaPrep LLC

The Surfer Health Study: Microbial Water Quality Measurements Supporting a CombinedWet Weather Surfer Epidemiology and QMRA Study in San Diego, CAPresented by: Joshua Steele, Southern California Coastal Water Research Project Quantitative Microbial Risk Assessment Case Study for Recreational Waters: Tecolote Creek, CA Presented by: Rachel Noble, University of North Carolina at Chapel Hill

EPA's Recreational Water Quality Criteria: Tools for Developing Site-Specific Alternative CriteriaPresented by: John Ravenscroft, US Environmental Protection Agency

BELLFLOWER

(cont.)

What would it take to comply with REC criteria during storms? Watershed-scale stormwater BMP modeling with cost-benefit optimizationPresented by: Dustin Bambic, Paradigm Environmental

The Impacts of Bacteriophage Recreational Water Quality Criteria on the Wastewater Treatment IndustryPresented by: Thomas Worley-Morse, Hazen and Sawyer

Should We Keep Our Heads in the Sand? Beach-Going Pathogens and Human HealthPresented by: Valerie (Jody) Harwood, University of Southern Florida

BELLFLOWER

7

DetailedSchedule

8:30 - 9:30 AM Verbal Presentations

9:45 - 10:45 AM Keynote Address - Grumman Auditorium

“Indicators of Microbial Quality of Waters: From D'Herelle to what the Hell” - Dr. Gary Toranzos

11:00 AM - Noon Verbal Presentations

Viruses

Identification of host-phage interaction network in Great Salt Lake reveals the roleof bacteriophages in bacterial diversity and populationPresented by: Ramesh Goel, The University of Utah

Human picornavirus diversity in archival water samples in the Netherlands: 1987-2012 Presented by: Wilemijn Lodder, RIVM

Development of a rapid enrichment and real-time PCR assay for the detectionof F+ DNA (Inoviradae) coliphages in waterPresented by: Yvonne Yuen, The University of North Carolina at Chapel Hill

REDBUD

Evaluation of a Candidate Bacteria Host for Simultaneous Detectionand Quantification of Somatic and Male-specific/F+ Coliphages in Reclaimed WaternPresented by: Matthew Price, The University of North Carolina at Chapel Hill

The detection of infectious viruses using a viability-PCR Presented by: Wilemijn Lodder, RIVM

Use of preservative agents for increase poliovirus survival on positively charged filtersPresented by: John Scott Meschke, The University of Washington

REDBUD

Distribution of fecal indicator bacteria around the city of Venice, Italy:temporal patterns, reservoirs and the role of tidal forcingPresented by: Gian Marco Luna, National Research Council of Italy

Long-term Changes in Vibrio abundance in the Neuse River Estuary of Eastern North Carolina Presented by: Brett Froelich, UNC Institute of Marine Sciences

Modelling the fate and transport of microbial pathogens in the Sediment ofthe Apies River, Pretoria, South Africa: A better understanding of the humanhealth risk associated with the use of surface waterPresented by: Eunice Ubomba-Jaswa, CSIR

BELLFLOWER

DetailedSchedule

REDBUD

Persistence of plasmids in antibiotic resistant stream water Escherichia coli harboringintegron, conjugation, and/or mobilization genesPresented by: Suhartano Suhartano

High prevalence of Multiple Antibiotic-Resistant (MAR) bacteria in riverbed sediments of the Apies River, South Africa: a possible health threat to populations living in resource poor settings Presented by: Akebe Luther King Abia

Environmental Reservoirs Of Antibiotic Resistance Associated with Small ScalePoultry Farming in Northwestern EcuadorPresented by: Karen Levy for Nikolay Braykov, Center for Global Safe Water at Emory

POU

Assessment of physico-chemical and microbiological quality of drinking water from disinfected water sources points to house hold water containers in selectedcommunities of Akaki-kaliti sub city, Addis Ababa City Administration Presented by: Mengestayhu Birhanu

Defining Best and Worst-Case E. coli Removals for Home Water Treatment Unit Tecolote Creek, CA Presented by: Gail Brion, The University of Kentucky

BELLFLOWER

Metagenomics to Unlock the Innovative Nitrogen Transformations in Aquatic Ecosystem:Denitrification Coupled to gaseous electron donors Presented by: Ramesh Goel, University of Utah

Distributed metabolism in suspended and attached growth anammox bioreactors revealedthrough metagenomic sequencing Presented by: Christopher Lawson, University of Wisconsin- Madison

Using qPCR as a Rapid Detection Method for Determining Nitrification and Data Incorporationinto a Nitrification Potential Index (NPI) in a South Texas Distribution System Presented by: Nikki Gordon, City of Corpus Christi

WINDFLOWER

Noon - 1:00 PM Lunch - Trillium Dining Room


DetailedSchedule

BELLFLOWER

How safe are improved drinking water sources in low- and middle-income countries?Evidence from systematic reviewsPresented by: Ryan Cronk, The Water Institute at UNC Community Led Diarrhea Eradication in Lower Nyakach, Kenya Presented by: Robert Metcalf, California State University, Sacramento

Safe Distances between Groundwater Based Water Wells Point and Pit Latrines atDifferent Hydrogeological Conditions in the Ganges Atrai Flood Plains of BangladeshPresented by: Md. Sirajul Islam, iccdr,b


3:15 - 3:45 PM Break

3:45 - 5:00 PM Keynote Address - Grummand Auditorium

“Right here, right now? The which, where and when of disease transmission barriers” - Dr. Huw Taylor

5:00 - 6:30 PM Poster Reception - Atrium

6:30 PM Conference Dinner - Trillium Dining Room

Influence of Wastewater Effluent on the Proximal Microbial CommunityComposition in Groundwater and SedimentPresented by: Carrie Givens, USGS Michigan Water Science Center

Development and performance of the Phytoxigene CyanoDTec Test: A rapid molecular assay for the routine monitoring and differentiation of toxin producing cyanobacterial blooms Presented by: Mark Van Asten, Diagnostic Technology

Sampling Approaches for Detection of Salmonella in Irrigation Ponds in Southern GeorgiaPoultry Farming in Northwestern EcuadorPresented by: Karen Levy, Center for Global Safe Water at Emory

REDBUD

Identification of human fecal pollution indicators by deep sequencing of sewagePresented by: Sandra McClellan, University of Wisconsin- Madison

Assessing fecal bacteria concentration and MST assay performance in gulland dog fecal samples from Wrightsville Beach, NC Presented by: Kellen Lauer, UNC Institute of Marine Sciences

Results from a Multi-Year Microbial Source Tracking Study in an Urban Watershed in MissouriPresented by: Rebecca Bushon, US Geological Survey

BELLFLOWER

DetailedSchedule

REDBUD

Interlaboratory Variability in Human-Associated Fecal Source IdentificationqPCR Conditions for Data AcceptancePresented by: Orin Shanks, US Environmental Protection Agency

Comparing qPCR and ddPCR microbial source tracking approaches in the complex setting of Tecolote Creek, CA Presented by: Emelie Andersson, UNC Institute of Marine Sciences

Degradation of Pathogens, General and Host-associated Fecal Indicators as Measuredby qPCR and digital PCR in FreshwaterPresented by: Yiping Cao, Southern California Coastal Water Research Project

Water Quality in an Urban Stretch of the Chattahoochee RiverPresented by: Lisa Casanova, Georgia State University Risk classification of Swedish Surface Source Waters Presented by: Karin Jacobsson, National Food Agency, Sweden

BELLFLOWER

8:30 - 9:30 AM Verbal Presentations

9:45 - 11:00 AM Keynote Address - Grummand Auditorium

Topic: Methods for the detection of viruses, including rapid, sensitive methods for the detection of infective viruses and the simultaneous detection of multiple infective viruses - Dr. Marylynn Yates

11:00 AM - Noon Side Events

Emerging Pathogens in WWTPS

DOGWOOD (continued)

11

DetailedSchedule8:30 - 9:30 AM Side Events

Thursday 5/21


Convened by University of North Carolina at Chapel Hill

DOGWOOD

BELLFLOWER

Wastewater

Minimizing disease transmission risk in emergency settings: laboratory-basedevaluation and optimization of two novel protocols for the in situ physico-chemicaldisinfection of pathogen-laden hospital wastewatersPresented by: Emanuele Sozzi, University of Brighton

The Use of Algal Biofilms for Decentralized Wastewater Treatment Presented by: Daniel Johnson, OneWater Inc.

Best methods for detecting C. perfringens in untreated and treated wastewaterPresented by: AJ Karon, The University of North Carolina at Chapel Hill

Noon - 1:00 PM Lunch


2:30 - 3:00 PM Break



DOGWOOD (continued)


DOGWOOD (continued)

DetailedSchedule

12

REDBUD

Using Human-Associated, DNA Biomarkers for Bacteroidales in a Simple, MultiparameterModel to Pinpoint Hotspots and Rank Fecal Sources in an Urban WatershedPresented by: Gail Brion, University of Kentucky

The use of remote sensing and geographic information systems in microbial source tracking Presented by: Jianyong Wu, University of North Carolina at Chapel Hill

Systematic literature reviews and development of distribution curvesfor norovirus and coliphages in raw wastewaterPresented by: Sorina Eftim, ICF International

11:00 AM - Noon Verbal Presentations

Ronald M. Atlas is Professor of Biology at the University of Louisville. He received his BS degree from the State University at Stony Brook, his MS and PhD degrees from Rutgers the State University, and a DSc (honoris causa) from the Universityof Guelph. He is a fellow in the American Academy of Microbiology. He was a postdoctoral fellow at the Jet Propulsion Laboratory where he worked on Mars Life Detection. He has served as President of American Society for Microbiology, as a member of the NIH Recombinant Advisory committee, as chair of NASA’s Planetary Protection Subcommittee, as chair of the Wellcome Trust Pathogens, Immunology and Population Health Strategy Committee, as co-chair of the American Society for Microbiology Biodefense Committee, as a member of the FBI scientific working group on microbial forensics, as a member of the DHS Homeland Security Science and Technology Advisory Committee and as chair of the Board of Directors of the One Health Commission. He is author of nearly 300 manuscripts on topics ranging from the cleanup of oil spills to infectious diseases and bioterrorism. He also has authored 20 books, several of which have been classic textbooks used around the world to educate the next generation of microbiologists. He currently is chair of the American Society for Microbiology Public and Scientific Affairs Board and regularly advises the White House and US Congress on issues of environmentand infectious diseases.

Gary A. Toranzos, Professor of Microbiology at the University of Puerto Rico, has been doing research in Environmental Microbiology since 1986. Toranzos received his Ph.D. from the University of Arizona (with Chuck Gerba) and did a couple of post-docs in Florida and Puerto Rico (Environmental Virology and Microbial Ecology respectively). His work on E. coli as an autochthonous microorganism in waters and his work on bacteriophages have been key in the search for new indicators of risk. Lately, his work on paleomicrobiology and the use of the microbiome as a marker of human ethnicity has caught a lot of attention.

Gary A. Toranzos, PhD Professor of Microbiology, University of Puerto Rico

SpeakerBios

Ronald Atlas, PhDProfessor of Biology and Public Health,University of Louisville

14

Huw Taylor is Professor of Microbial Ecology at the University of Brighton in the UK, where he leads Environment and Public Health research within the Aquatic Research Centre. He has twenty five years' experience of public health research and currently leads a £3.65 million EU Interreg project (RiskManche) that is investigating the fate and transport of pathogens in river catchments and coastal environments. He is an expert advisor to the WHO on the development of Sanitation Safety Planning, drinking water quality analysis and waterborne disease management. In recent years, he has advised the WHO on the WASH response to the Ebola outbreak. He has also worked closely with UNICEF on an improved evidence base the provision of safer water from low-cost water supplies in Malawi and with Doctors Without Borders, Oxfam and other NGOs on novel approaches to the disinfection of wastewaters in emergency settings.

Huw Taylor, PhDProfessor of Microbial Ecology, University of Brighton

Marylynn V. Yates is Professor of Environmental Microbiology and Distin-guished Teaching Professor at the University of California, Riverside. She is Chair of the University of California Global Health Institute’s Education Committee. Dr. Yates holds a B.S. in Nursing from the University of Wiscon-sin, Madison, an M.S. in Chemistry from the New Mexico Institute of Mining & Technology, and a Ph.D. in Microbiology from the University of Arizona. Her research interests include characterizing and predicting the fate and transport of human enteric pathogenic microorganisms in soils, water, and wastewater; development of methods for rapid, sensitive detection of infective enteric viruses in water samples; human pathogen considerations associated with wastewater reuse and biosolids application to land; and the use of indicators for predicting pathogen occurrence and behavior in the environment. Dr. Yates serves as a member of the Water Science & Technology Board of the National Research Council, on the USEPA’s Science Advisory Board Drinking Water Committee, and on the American Society for Microbiology’s Council Policy Committee. She is a fellow of the American Association for the Advancement of Sciences, the American Academy of Microbiology, and a National Associate of the National Academies of Science.

.

Marylynn V. Yates, PhD Professor of Environmental Microbiology andDistinguished Teaching Professor,University of California, Riverside

SpeakerBios

15

Convened by the University of North Carolina at Chapel Hill’s Institute of

Marine Sciences and the Department of Environmental Sciences and

Engineering

Session format: Training or Learning WorkshopSession output: Other

Room: Redbud Session: Monday AM Contact: Rachel Noble

Human pathogenic enteric viruses such as adenovirus, enterovirus, and norovirus, are prominently found in human sewage, and have been identified as important causative agents of gastroenteritis in humans from exposure to contaminated recreational waters and consumption of contaminated shellfish. Human pathogenic enteric viruses have very low infectious doses (as low as 1-10 virus particles), are highly transmissible, and have biochemical characteristics that permit them to persist in coastal receiving waters and shellfish, making these viral pathogens a serious concern. Previous work has suggested that currently utilized fecal indicator bacteria (FIB), such as E. coli and Enterococcus, do not adequately predict the presence of human viral pathogens in receiving waters that arecontaminated with human sewage, meaning FIB are ineffectual proxies for pathogeni c viruses.

To date, methodological limitations prevent the desired multiple target viral pathogen analysis that could provide vital information for controlling specific viral pathogens of concern. A range of issues prevent human viral pathogens from being easily and directly quantified in sewage effluent, stormwa-ter, and coastal receiving waters. Coliphage (bacteriophage that infect E. coli bacteria) have been previously cited as having potential to be used as fecal indicator viruses (FIV). Coliphage benefit from being quantifiable in a range of water types, and specific subgroups of coliphage (e.g. F+ coliphage) have been identified as having significant relationships to human health outcomes in recent epidemio-logical studies conducted in Southern California. Based upon the previously raised issues, federal agencies and groups including USEPA, FDA, and ISSC have highlighted possible routes forward to include coliphage in water quality and shellfish harvesting water quality management plans, including the possible development of new coliphage-based criteria for recreational waters, and new regulations regarding shellfish harvesting water closure areas based upon coliphage.

This side event is being convened on the topic to accomplish the following objectives:1) Provide an overview of current activities of federal agencies on the topic of coliphage and coliphage related water quality management strategies2) Provide a state of the science review, covering a range of topics of international interest on coliphage, and human enteric viral pathogens3) Identify, using a structured, prioritized, and facilitated approach, the existing data gaps to implementing water quality management strategies4) Identify short-term and long-term strategies to satisfy data gaps

Monday 5/18

SideEventDescriptions

16

Convened by the University of Connecticut

and the Water Institute at UNC

Session format: Education PresentationsSession output: Future Collaboration, Other

Room: Bellflower Session: Monday AM Contact: Jonathan Mellor and Kristen Downs

Recent estimates indicate that anthropogenic climate change may increase the relative risk of diarrhea by 22!29% by the end of the 21st century (Kolstad and Johansson, 2011). Despite this, little attention has been paid to this potential crisis in the water sector and there are major gaps in our understanding of how climate change and weather variability will impact waterborne and foodborne diseases. Most studies conducted to date inadequately address modifying environmental or social factors, mechanisms, or the complexity of the underlying science that link climatic factors to waterborne and foodborne diseases. The understanding of these mechanisms and can only be achieved by bringing together a broad range of scientists and practitioners from the climatological, epidemio-logical, microbiological, agricultural, hydrological, social, and engineering sciences.

Therefore, the University of Connecticut and the UNC Water Institute are hosting a side event at the2015 Water Microbiology Conference covering the climate drivers of water! and foodborne pathogens and the illnesses they cause. It includes presentations from experts in multiple disciplines who use a variety of methods and data sources to study the linkages between climate, water, and health across urban, freshwater, and agricul-tural environments in developed and developing countries. The outline of the side event is as follows:

Presentations: Dr. Chip Konrad (University of North Carolina at Chapel Hill) | Characteristics of Weather Events

and Variation in the Context of Climate Change and Health Research Dr. Kathleen Alexander (Virginia Tech) | The Complexity of Diarrheal Disease:

Climate Change and Population Vulnerabilities in Low-resource Countries Dr. Tim Wade (US Environmental Protection Agency) | Extreme Precipitation and Emergency Room Visits

for Gastrointestinal Illness in Areas with and without Combined Sewer Systems Cynthia Lin (University of North Carolina at Chapel Hill) | Flooding and Clostridium difficile infections:

A Case-Crossover Study Dr. Sandra McLellan (University of Wisconsin!Milwaukee) | Waterborne Disease and Weather in the Great Lakes:

A Population Genetics and Ecology Perspective Dr. Jeanette Thurston (US Department of Agriculture) | Climate Impacts on Agricultural Sources

of Water-related Diseases Dr. Jiyoung Lee (Ohio State University) | Extreme weather-Water-Food Linkage: Impact on Human Health

Ultimately, we envision this side event will further focus efforts and build partnerships around this critical subject and propose innovative approaches to advance the science and understanding of climate impacts and adaptation strategies.

17

Convened by Georgia State University

Session format: Training or Learning Workshop Session output: Publishable Paper or Other Framework

Room: Bellflower Session: Monday PM Contact: Lisa M. Casanova

Writing up your work for publication can be one of a scientist’s biggest challenges. Your career advancement, and the advancement of your field, depends on getting your work into the peer-reviewed literature. Do you have projects you need to write for publication? Does the writing process seem overwhelming and intimidating? Are you struggling with organization and presentation of your ideas? Do you have writing in progress that isn’t coming together the way you want? Whether you are just starting research or ready to write, this interactive workshop will help you make progress on your paper with a framework for organizing your writing, and help you work toward the goal of a publishable paper by tackling all stages of your writing process:

Convened by Artel

Session format: Training or Learning WorkshopSession output: Proceedings

Room: Windflower Session: Monday PM & Tuesday AM Contact: Emily Avis

This one and a half hour training session provides a quick and easy way to gain knowledge on proper pipetting technique. In addition to learning how pipettes work and fail, the audience will be trained on how to standardize proper pipetting technique, and recognize ergonomic risk factors and sources of stress.

Participants will have the tools necessary to improve their pipetting skills, the knowledge to avoid the most common injuries associated with pipetting, and an understanding of the mechanics and performance character-istics of mechanical pipettes.

Measurable Learning Objectives:

Bring your questions and manuscript writing projects at any stage. This workshop is highly interactive- we will work with your specific examples to help you organize and plan your writing. This workshop is for scientists at any stage of the writing process, whether you are just starting or in the process of writing a manuscript.

Convened by WEF Disinfection and Public Health Committee

Session format: Training or Learning WorkshopSession output: Future Collaboration

Room: Redbud Session: Tuesday AM Contact: Jay Swift

In this side session, the fundamentals of disinfection of reuse water, wastewater and biosolids will be discussed. The efficacy of pathogen and indicator inactivation for both established methods and emerging technologies will be presented.

Technologies discussed for wastewater and water reuse include chlorine-based disinfection, UV, ferrate, peracetic acid, ozone and pasteurization. For many years, chlorine-based disinfection dominated wastewater disinfection in the U.S. However, the use of UV systems has grown dramatically in the past 20 years, due to the development of higher-efficiency UV lamps, improvements in reactor design and automation, and the absence of byproducts in the disinfected effluent. Ferrate and peracetic acid are gaining interest in the wastewater industry, achieving disinfection with reduced formation of disinfection byproducts. Interest in ozone disinfec-tion has also been increasing, due to advances in ozone generation systems and contactors, as well as ozone’s strong reactivity and its potential to improve water quality through removal of trace organics. Pasteurization is a new and emerging approach that has been used for disinfection of reuse water in California.

For biosolids, established technologies such as alkaline stabilization and heat pasteurization, along with new and emerging methods will be presented.

For each disinfection technology, current research regarding the factors impacting the efficacy of microbial inactivation will be discussed, including for established and emerging pathogens, including viruses, bacteria and protozoa. Multi-barrier approaches will also be discussed.

The session will end with a round table discussion to facilitate the identification of research needs and future collaboration.

Tuesday 5/19

Pathogenic Environmental Mycobacteria: Detection, Sources,

Convened by US Environmental Protection Agency

Session format: Education PresentationsSession output: Future Collaboration, Other

Room: Bellflower Session: Tuesday AM Contact: Al Dufour

Mycobacterium avium complex is an environmental water-based pathogen that is posing an ever-increasing worldwide health risk to human populations. The purpose of this side event is to bring the latest scientific information regarding various aspects of environmental Mycobacterium avium complex to the attention of the scientific and policy-making communities. The goal is to stimulate the interest of meeting attendees in this research area by encouraging the exchange of scientific information and, furthermore, to promote new research interest in this pathogen.

Countries to Eliminate Waterborne Disease

Convened by International Water and Health Alliances

Session format: Training or Learning WorkshopSession output: Future Collaboration

Room: Dogwood Session: Tuesday AM Contact: Robert Metcalf

WHO’s estimate of the daily toll of waterborne diseases is >4,600,000 episodes of diarrhea and >2,000 deaths. The majority of these episodes and deaths are among people in extreme poverty who live in rural areas of developing countries and use unimproved, contaminated drinking water sources. This workshop will cover practical approaches to water testing, community teaching and disinfection methods in low resource areas that communities can adopt to reduce the plague of waterborne diseases.

Two water testing methods will be covered: 1) the Portable Microbiology Laboratory (PML), a field kit that contains two tests widely used in the water and food industries to detect the fecal indicator E. coli. 2) The Aquagenx portable and self contained Compartment Bag Test (CBT) for E. coli. Workshop participants will perform these tests on water sources to obtain next day results that correlate with WHO disease risk guidelines.

Using the Friends of the Old (FOTO) project in Lower Nyakach, Kenya, as an example, a community teaching strategy will be presented that demystifies microbiology and clarifies the link between contaminated water and disease, replacing myths with science. Practical disinfection methods of liquid chlorine and solar water pasteuri-zation used in the FOTO project will be covered.

Thursday 5/21Emerging Pathogens in WWTPS

Convened by the University of North Carolina at Chapel Hill

Session format: WorkshopSession output: Proceedings

Room: Dogwood Session: Thursday Contact: Jacqualine MacDonald Gidson

The past ten years have seen the rapid growth of new molecular methods that enable the identification and enumeration of pathogens and have the potential to help mine the 'dark matter' of microbial communities for new public health tools. However, in emergency disease outbreak settings these developments have had very little impact on water, sanitation and hygiene practice. The 2010 cholera outbreak in Haiti and the recent West Africa Ebola outbreak both demonstrated the knowledge gaps around dealing with pathogen-laden human feces. There is a critical need for rigorous and integrated analytical methods, decision support tools, and emergency preparedness protocols to help for water and wastewater engineers, public utilities, hospitals, health officials, and community leaders to be prepared for the possibility of such high-risk pathogens appearing in wastewater and to prevent potential exposures.

This workshop will produce first draft emergency preparedness protocols for managing the risks of pathogen transmission via fecal matter and wastewater in outbreak settings. Three protocols for managing fecal matter will be prepared: one for outbreak communities, the second for hospitals and other treatment centers, and the third for wastewater treatment plants.

The workshop will bring together participants from wastewater utilities, hospitals, public health agencies, and non-government organization personnel with experience in outbreak settings. In addition, participants will include infectious disease experts and experts in decision-making under emergency situations.

During the morning session of this full-day workshop, experts will present key topics relevant to emergency preparedness protocols for fecal waste management in outbreak settings. Then, participants will divide into three break-out groups focused on managing fecal matter in (1) outbreak communities, (2) hospitals and other treatment centers, and (3) wastewater treatment plants. Each group will write a first draft of an emergency preparedness protocol relevant to the particular setting. Subsequently, these drafts will be circulated among the groups for revision and expansion.

21

Title: Presenter:

Using molecular assays and other water-quality and environmental variables Donna Francy, U.S. Geological Surveyto predict harmful cyanobacteria blooms in freshwater lakes (presented by Rebecca Boushon)

Best Practices in the Use of Micropipets Candie Gilman, Artel (presented by Emily Avis)

Biogeographic patterns of Escherichia coli, Enterococcus spp., Curtis Stumpf, Crystal Diagnosticsand Bacteroidales fecal indicator bacteria at a Villa Angelaand Euclid Creek Beach, Ohio

Toward a rapid enzymatic method for detection of coliform bacteria in water Jianyong Wu, University of North Carolina at Chapel Hill

Assessment of bacteriological quality and management practice Mengestayhu Birhanu Akelatof drinking water at their ground water sources in Akaki Kaliti sub-city,Addis Ababa City Administration

Understanding the Role of Soil in Zoonotic Pathogen Transmission and Water Seungjun Lee, Ohio State UniversityContamination in Cameroon using One Health Approach

Balancing Environmental Stewardship and Cost in the Disinfection Isaiah Shapiro, Greeley and Hansen of Wet Weather Flows in Water Resource Recovery Facilities

Fecal indicator bacteria in riverine sediments and overlying waters Gian Marco Luna, National Research Council of Italybefore and after a storm event

Strengthening the Capacity of Households and Communities Charles Okyere, University of Bonn,for Improved Water, Sanitation and Hygiene: Water Testing Experiments Center for Development Researchwith School Children

Microbial and turbidity removal by chitosan coagulation to optimize Lydia Abebe, University of North Carolina at Chapel Hillceramic water filtration for household drinking water treatmentand Adult Household Members in Ghana

Scientific Basis for Defining Microbiological Health of Chicago River System Geeta Rijal, Metropolitan Water Reclamanation District of Greater Chicago

Fecal Indicator Bacteria in Sachet Water Collected from the Streets of Ghana Asli Aslan, Georgia Southern University

Comparative genomics of rhodococcus opacus strain M213 reveals potential Ashish Pathak, Florida A&M Universityfor bioremediation and lipid-biofuels production

The Affect of Pause Time on the Efficiency of Light Biosand Filters Jennifer Mendell, Bridgewater State Universityin Siem Reap, Cambodia

22

Title: Presenter:

Modeling Household's Decisions on Water Supply and Sanitation Charles Okyere, University of Bonn,in Greater Accra Region of Ghana Center for Development Research

Antimicrobial Resistance: Environmental Pathways and Impact on Human Health Lydia Abebe, University of North Carolina at Chapel Hill

Testing Pathogen Reduction in Deployed Composting Toilets Erika Keim, University of Washington

Salmonella transport from pond water sources through irrigation systems Moukaaram Tertuliano, University of Georgiaon mixed produce farms in the southeastern United States

Exposure to human source fecal indicators and self-reported illness among Melanie Napier, University of North Carolina at Chapel Hillbathers at recreational beaches

Culture-independent method for source determination of fecal wastes Richard Haugland, US Environmental Protection Agencyin surface and storm waters using reverse transcriptase-PCR detectionof FRNA coliphage genogroup gene sequences

Survival and Transport of Salmonella in Soil Microcosms Debbie Lee, Emory University

Streamlining viral concentration methods Roberto Rodriguez, University of Texas Health Science Center

Evaluation of CHROMagar Clinical Diagnostic Culture Media for Direct Kathleen Brown, University of North Carolina at Chapel HillDetection and Enumeration of Antimicrobial Resistant E. coli and Coliformsin Sewage and Other Environmental Samples

Potential for small solar water pasteurizer techniques for water disinfection Tayyebatossadat Pour Seyed Aghaei,in rural area of developing countries Solar Clean Water Solution

Geostatistical prediction of microbial water quality on an urbanizing David Holcomb, University of North Carolina at Chapel Hillinland stream network

Capture and concentration of waterborne parasites - current limitations Ewelina Wachnicka,& opportunities for risk assessment Unilever Safety & Environmental Assurance Centre

Simple, Low Cost and Ready-to-use microbiological test platform Bob Salter, Charm Sciences

An assessment of the risk of infection by Cryptosporidium parvum Ileana Wald, University of South Floridaand Giardia lamblia from exposure to livestock waste and tubulardigester effluent in Costa Rica

develops, manufactures, and markets a wide range products used for research in functional genomics, proteomics, and food safety. The group ranks among the top five life science companies worldwide, and maintains a solid reputation for quality, innovation, and a longstanding focus on the success of its customers. Bio-Rad’s life science technologies include electrophoresis, imaging, multiplex immunoassay, chromatography, microbiology, bioinformatics, protein function analysis, transfection, flow cytometry, and nucleic acid amplification. Bio-Rad’s Digital Biology Center specializes in the development of advanced technologies for the rapidly growing field of digital biology – including complete solutions for droplet digital PCR and digital sequencing.

is an independent, third-party testing and certification for evaluationof product performance and contaminant reduction claims.

OurSponsors

Compartment Bag Test (CBT) is a simple, portable water quality test kit that lets anyone, anywhere determine if drinking water contains E. coli bacteria and poses a health risk. It is ideal for on-site testing in low resource and disaster settings because it generates quantified test results without requiring electricity, labs or extra equipment such as incubators and UV lamps. The CBT’s simplicity and convenience remove the barriers for bacteriological water quality monitoring in any location where testing was previously too difficult or costly.

provides quality assurance systems and a broad and fully customizable range of support, training and consultation services for laboratories using automated liquid handling instrumenta-tion and manual pipettes.

microbial indicator tests for food/water quality, Fast Phage (somatic & male-specific coliphage) and E*Colite (E.coli/coliform), are EPA accepted. Now introducing new Peel Plate® test platforms for heterotrophic and E.coli/coliform quantification.

provides novel tools for effortless and rapid sample preparation and concentra-tion of waterborne bacteria, protozoa, viruses or other microorganisms from large volumes. This technology enables cutting-edge detection instruments to contribute their full potential for same day results. Please visit our booth for a demonstration of the InnovaPrep Concentrating Pipette – the first truly automated liquid to liquid concentrator.

Bawell is a manufacturer of kitchen counter top water electrolysis machines. Bawell water ionizers produce electrolyzed reduced drinking water by separating the alkaline and acidic minerals present in water. Electrolyzed reduced water has been extensively researched and found to have many health improving qualities not present in ordinary drinking water.

AboutUs

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The Water Institute at UNC provides global academic leadership for sustainable management

of water for health and human development. Based in UNC’s Gillings School of Global Public

Health, one of the highest ranked public health schools, we work to solve the complex global

water challenges through our four main strategic functions: research; teaching and learning;

knowledge and information management; and networking and partnership development. We

have seven focus areas to support these functions, which are: monitoring, evaluation and

learning for WaSH; WaSH governance; sanitation for the 21st century; adapting to water

scarcity and climate change; drinking water for all; national and regional WaSH challenges in

the U.S.; and the water-food-climate-energy nexus.

Through all of our endeavors, we work towards improving access to safe water, sanitation and

hygiene for all. We unite faculty, students, practitioners, and policymakers around this

common purpose on a local, national and international scale.

Documents

Download the conference agenda (PDF)