DOUBLE BEAM UV VIS

SPECTROPHOTOMETER

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Lab Systems & Biotech (I) Pvt.Ltd., pioneers in spectroscopy field since last 30 years with its rich core competency in sales & support of spectroscopic analytical instruments is proud to introduce a very powerful, rugged, reliable yet economical and very

plus easy to use true double beam UV VIS spectrophotometer Model LAB UV 3000 PC.

plus LAB UV 3000 PC spectrophotometer with its wide sample compartment accommodates various accessories like multi cell holder, micro cell holder, solid sample holder, thermostated cell holder, variable path length cell holder, sipper system, etc to cater wide range of applications in the field of pharmaceuticals, life sciences, chemicals, petrochemicals, agriculture, food & beverages, environmental, clinical & diagnostics, educational institutes as well as quality control, research & development laboratories.

We believe that there is neither greater compliment nor there is a greater asset than a company can have a truly satisfied customer. We value your suggestions, appreciate your recommendations and give them serious consideration. We value your views as our customer because our goal is customer satisfaction.

We achieve this goal through a combination of quality of innovative products and dedicated technical staff.

We believe, we have many satisfied & reputed customers. We can name a few like Ajanta Pharma, Akriti Pharma, Adley Pharma, Admac Pharma, Aarti Drugs, Alkem Laboratories, ARAlL Ankleshwar, Analab, Apar, Abbott, Aptuit Laurus, Amoli, Aristo, Bayer, Bee Pharmo Testing Lab., Biochem, Biomab, Bombay Tablet, BARC, Cachet Pharma, Citochem, CSIO, CFTRI, CAT, Cheminnova, CEERI, Cipla, Cadila, Clariant, Ciron, Coral, Choksi Lab, Dishman, Dr. Reddys Lab., Deepak Fertilizer, Diamond Dyechem, Dabur, Enicar Pharma, Emcure, Elder, EthyPharm, FDC, Forensic Science Lab., Griffon, Gelnova, Haffkine Institute, Hetero, Hikal, Hexa Analytical Lab, ICPA Health Care, Indchemie, Impulse Pharma, Invochem Laboratories, ITC, Intas, Ipca, Jayco Chemicals Ind., Kopalle Pharma, KEM Hospital, Lupin, Maxwell Lifescience, Mankind, Matrix-Mylan, Maneesh Pharma, Medinex, M.J.Lab, Mumbai University, NIO-Goa, NIPER, Oscar Labs, Olive Healthcare, Orchid, Okasa, Plethico, P&B Pharma, PERD Centre, Pricise Pharmachem, Promed, Pharmasolve, Ranbaxy, Rubicon, Ratio Pharm, Raptakos Brett Test Laboratories, Rusan, RCA Lab, Sun Pharma, Saphire Lifescience, Sitec, Svizera, Srikem, Strides Arco, Symbiotec, Shreya Lifesciences, Sri Krishna Keshav, Transpek Ind., Torrent,Tulip,Tirupati Life Sciences, Textiles Committe, UICT, Venus Remedies, Vapi Care, VVC Pharma, Wockhardt, Wyeth, Zydus Cadila Pharma, Zibran Pharma, Zeiss Pharma, Etc.

We thank all our valued customers for trust and co-operation in working with our company and making it what it is today and will be in future.

We have state of the art, full range of analytical instruments complying 21 CFR Part 11 as required by regulatory authorities like US FDA, EP, BP, TGA, DAB, MHRA, JP Pharmacopoeias along with IQ/OQ/PQ documentation.

Our well qualified and factory trained engineers and application specialists are always eager to respond quickly and efficiently to the need at our customers.

INTERNAL DESIGN

Technical Specifications:

The two detectors measure sample and reference

light respectively and simultaneously for

optimizing measurement accuracy. It provides

excellent performance for measurements in the

range of 190 to 1100 nm. It is suitable for

pharmaceutical, biochemical and clinical lab

applications as well as routine applications such as

quantitative analyses, kinetics, wavelength scan,

multiple components and DNA/Protein. UV VIS

Analyst software makes this instrument versatile.

It provides excellent performance for

measurements.

SALIENT FEATURES :

• True Double Beam with 1nm fixed bandwidth.

• Fixed bandwidth for reproducible results.

• High quality Silicone Photo-diode detectors.

• In Stand-alone mode, all software methods are

included as built-in standard; this eliminates

the need of software.

• On line display of UV and visible lamps life on

the screen at a glance.

• The status of the lamps life ensure that the user

can change lamps conveniently.

• I

• Online software upgrade via internet helps to

keep it updated.

• Data Download to PC software expands the data

storage to unlimited.

• Pre-aligned Deuterium and Halogen lamp for

easy lamp replacement.

• Built-in self check and auto Diagnostics facility.

• Buit-in System Validity.

• Complies EP/BP/USP Pharmacopoeia test for

Stray Light, Resolution, etc.

ncludes windows based application software.

Model

Wavelength Range

Spectral Bandwidth

Optical System

Grating

Wavelength Accuracy

Wavelength Repeatability

Wavelength Display / Setting

Wavelength Scanning Speed

Wavelength Scanning Step

Photometric Range

Photometric Accuracy

Photometric Repeatability

Photometric Baseline Stability

Photometric Baseline Flatness

Photometric Noise

Stray Light

Measuring / Scan Ordinate Modes

Operation Mode

Display

Sample Compartment

Detector

Light Source

Output

Power Requirement

Dimension

Weight

Additional Software Module

Environmental Requirement

Data Download to PC

plus LAB UV 3000 PC

190 nm - 1100 nm

1.00 nm Fixed Over Entire Range

True Double Beam

Holographic with 1200 Lines/mm

±0.1 nm - UV Region

±0.1 nm @ 656.1 nm D2 line

±0.3 nm - Visible Region

±0.1 nm – Over Entire Range

±0.1 nm - Increment

HI,MED,LOW,VERY LOW (3000-1 nm/min)

0.1 – 5.0 nm

-0.4 Abs to +4.0 Abs., 0-400% T

±0.001 Abs @ 0.3 Abs

±0.002 Abs @ 0.5 Abs

±0.004 Abs @ 1.0 Abs

±0.006 Abs @ 2.0 Abs

±0.001 Abs @ 0.3 Ab

±0.001 Abs @ 0.5 Abs

±0.001 Abs @ 1.0 Abs

±0.003 Abs @ 2.0 Abs

≤0.0002 Abs/Hr @ 500nm, (After 1hr warm-up)

±0.0005 Abs Over Entire Range

≤0.00005 Abs RMS @ 500nm

<1.0 %T KCL @ 198nm,

<0.02 %T Nal @ 220nm,

<0.02 %T NaNO2 @ 340nm

Absorbance (Abs), Percent Trans (% T) & Conc. (C).

Stand-alone & PC Control.

5" Large LCD Screen – (320 X 240 Pixels)

W-155mm X D-250mm X H-115mm

High Quality Dual Silicon Photodiodes

Pre-aligned Tungsten & Deuterium Lamps

USB Port for PC & Parallel Port for Printer

AC-110/220V, 50/60-Hz

L-24" X D-18" X H-8"

20 Kg.

Kinetic and DNA/Protein

Temp. : 20°C - 35°C, Rh. : 20% - 85 %

Through licensed version UV Vis Analyst software

plus LAB UV 3000 PC

LAB UV Local Control Softwareplus 3000 PC All methods are included as built-in standard; this eliminates the

need of software & online software update via Internet.

The local control software includes functions such as:

Photometry, Quantitative, Wavelength Scan, Kinetics.

DNA/Protein, Multi-Wavelength Test and System Utilities.

WL: 500.0 nm 08:00:00

Cancel Move Dn Select

D2

WLAB

Main Menu

Basic Mode

Quantitative Mode

Wavelength Scan

Kinetics Mode

DNA/Protein Mode

Multi-Wavelength Mode

System Utility

1

2

3

4

5

6

7

WL: 500.0 nm 08:00:00

Method Standard Show Curve

D2

WSetup Standard Conc.

ID

1

2

3

4

Params

WL (nm)500.0

Search

Conc. (ug/uL)

1.000

2.000

3.000

Abs.

4.000

Up to 10 standard solutions may be used to establish calibration

equation curve. There is a choice of four methods for fitting

curve through the calibration points: Linear fit, Linear fit

through zero, square fit and cubic fit.

Standard Curve

WL: 500.0 nm 08:00:00

100.5

Abs.

0.0

000

230.0 Wavelength (nm) 650.0

Setup Mode Search Smooth

D2

W

Start WL680.0 nm

End WL200.0 nm

X Scale

Y Scale

Interval1.0 nm

The Wavelength Scan intervals are 0.1, 0.2, 0.5, 1, 2, 5nm, and

High, Medium and Low scan speeds are available. Scan speeds

vary from 100 to 1000 nm/min. Wavelengths are scanned from

high to low so that the instrument stand-by at high wavelength.

This minimizes the degradation of UV sensitive samples. Precise

control of filter and lamp changes means that their effects are

not seen on the final scan. Post-run manipulation includes re-

scaling axes, curve tracking and peak picking.

Wavelength Scan

WL: 500.0 nm 08:00:00

100.5

%T

99.5

0 Time 180

Setup Mode Smooth Search

D2

W

Total180s

Interval1s

X Scale

Y Scale

This mode may be used for time course scanning or reaction rate

calculations. Abs Vs Time graphs is displayed on the screen in real

time.

Wait time and measurement time up to 12 hours may be entered

with time intervals of 0.5, 1, 2,5, 10, 30, seconds and 1 min. Post-run

manipulation includes re-scaling, curve tracking and selection of

the part of the curve required for the rate calculation. Rate is

calculated using a linear regression algorithm before multiplying by

the entered factor.

Kinetics

WL: 400.0 nm 08:00:00

WL Setup

D2

WSetup Standard Conc.

ID

1

Mode

WL (nm)500.0

Search

Conc. (ug/uL)

200.0

300.0

400.0

Abs.

2.322

2.123

2.034

Scroll

Up to 10 wavelengths may be entered, allowing the measurement of

multiple wavelengths on a series of Samples.

Multi-Wavelength

WL: 320.0 nm 08:00:00

Coeff

D2

WDNA / Protein Test

No.

Method

WL (nm)260.0280.0320.0

Items

A1

A2

Aref

C-DNA

C-Pro

Ratio

Unit

Abs

Abs

Abs

ug/UL

ug/UL

Result

0.251

0.243

0.095

4.524

110.8

1.059

Unit Default

Concentration and DNA purity are calculated Absorbance ratios at

260nm/280nm or 260nm/230nm with optional subtracted

absorbance at 320nm.

DNA Concentration = 62.9*A -36.0*A Or 49.1*A -3.48*A260 280 230

Protein Concentration = 1552*A -757.3*A Or 183*A -75.8*A260 280 260 230

Other wavelengths and factors may be entered.

260

DNA/Protein Test

plus LAB UV 3000 PC

UV VIS Analyst Software for LAB UV plus 3000 PC

The Windows based PC application software UV VIS Analyst takes

the best features of the stand-alone version plus more powerful

data processing, expanded data collecting, and storage pluscapability. It is standard with LAB UV 3000 PC model and is

optional to stand-alone model.

The UV VIS Analyst software offers:1 . Photometric Mode

2. Quantitative Test (standard curve)

3. Wavelength Scan

4. Kinetics

5. DNA/Protein

6. Multi-Wavelength

7. System Utility

Use up to 20 standards to establish standard curve. Four

methods for fitting a curve;

1. Linear fit

2. Linear fit through zero

3. Square fit

4. Cubic fit

Quantitative Test (Standard curve)

Automatically records peaks and valleys. The quantity of

spectra is unlimited; you can simultaneously store as many

as desired. Post-run manipulation and processing includes:

1. Re-scaling axes, curve

2. 1st to 4th derivative

3. Smoothing, combination, zooming, overlap

Wavelength Scan

Kinetics (Abs Vs Time)The Kinetics mode may be used for time course scanning or

reaction rate calculations. Abs Vs Time graphs are displayed on

the screen in real time. Waiting time, measurement time and

time intervals may be entered. Post-run manipulation includes

re-scaling, curve tracking and selection of the part of the curve

required for the rate calculation. Rate is calculated using a

linear regression algorithm before multiplying by the entered

factor.

DNA/ProteinConcentration and DNA purity are quickly and easily calculated:

Absorbance ratios at 260nm/280nm with optional subtracted

absorbance at 320nm.

DNA Concentration = 62.9*A -36.0*A260 280

Protein Concentration = 1552*A -757.3*A260 280

Other wavelengths and factors may be entered.

Multi-WavelengthUp to 20 wavelengths can be selected and multiple samples can

be measured. (Auto cell changer is required to run multiple

samples automatically)

plus LAB UV 3000 PC

4-CELL HOLDER FOR 10mm- CUVETTERECTANGULAR

4-CELL HOLDER FOR UP TO 50mm- CUVETTERECTANGULAR

4-CELL HOLDER FOR UP TO 100mm- CUVETTERECTANGULAR

CYLINDRICAL CELL HOLDER

WATER-JACKETED CELL HOLDER

MICRO CELL HOLDER

TEST TUBE HOLDER

8-POSITION AUTO CELL CHANGER

SOLID SAMPLE HOLDER (SINGLE CELL)

10mm WATER-JACKETED 4-CELL HOLDER

Optional Accessories

CONST. TEMP. PELTIER SIPPER SYSTEM

CONST. TEMP. PELTIER SYSTEM

AMBIENT SIPPER SYSTEM

THERMAL PRINTER

DEUTERIUM LAMP

RECTANGULAR CUVETTES-GLASS/QUARTZ

MICRO CELL-QUARTZ

SEIF MASKING CONT. FLOW THROUGH GLASS/QUARTZ CELL

HOLMIUM OXIDE FILTER

HALOGEN LAMP

100

200

500

µlµlµl

10mm20mm30mm50mm

100mm

5mm10mm20mm30mm

Optional Accessories

329, Mastermind IV, Royal Palms, Aarey Milk Colony, Goregaon (East), Mumbai-400 065, India.Tel.: ++91-22-3251 4134, Telefax :++91-22-28794637/36, Website : www.labsystems.inE-mail : labbiotech@mtnl.net.in/labsystems@vsnl.com

:BRANCHES:

Baroda, Indore, Baddi, Goa, Hyderabad, Chennai

:Authorised Representative:

S u p p o r t B e y o n d S e r v i c e sNote : Lab Systems & Biotech (I) Pvt. Ltd. reserves the right to change specification without notice.

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