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Do Now—6.4.15
Turn your Do Now into the front
What grade do you think you earned on your final? Why?
Why grade do you think you earned on the EOC (on a score from 1-9)?
Ms. C’s Absence
Hi, I’ve been admitted to the hospital. My wound hasn’t healed and the doctor saw the hardware this morning (not good). I’m going to have surgery to have the hardware removed and get IV antibiotics.
I’ll be back when I can.Grades are as up to date as possible.
Objective
SWBAT analyze DNA profiles created by gel electrophoresis.
SWBAT practice micropipetting and loading gels.
DNA Profiling / DNA Fingerprinting
http://learn.genetics.utah.edu/content/labs/gel/
PCR
The polymerase chain reaction (PCR) is used to amplify specific regions of the DNA. The process uses the same enzymes used by cells to replicate DNA and short sections of DNA that act as primers
PCR
Forensic Investigation: Suspect DNA should be a complete match with the sample taken from a crime scene if a conviction is to occur
Restriction Fragment Length Polymorphism
Restriction enzymes cut DNA at specific spots that are nucleotide sequence-specific. The different cutting pattern will lead to differently sized products that can be separated in gel electrophoresis.
Gel Electrophoresis
Gel Electrophoresis
Gel Electrophoresis
Gel Electrophoresis
DNA is negatively charged
Smaller DNA fragments travel further during electrophoresis.
DNA Profiling
Paternity Testing: Children inherit half of their alleles from each parent and thus should possess a combination of their parents alleles
DNA Profiling
Paternity Testing: Children inherit half of their alleles from each parent and thus should possess a combination of their parents alleles
DNA Profiling
Forensic Investigation: Suspect DNA should be a complete match with the sample taken from a crime scene if a conviction is to occur
DNA Profiling
Forensic Investigation: Suspect DNA should be a complete match with the sample taken from a crime scene if a conviction is to occur
DNA Profiling
Forensic Investigation: Suspect DNA should be a complete match with the sample taken from a crime scene if a conviction is to occur
Classwork
Complete the worksheet to the best of your ability…
*This worksheet shows single stranded DNA
*This restriction enzyme makes a blunt cut
WorksheetStandard
CCATCCAAGACATTATGCAGG/CCTAGACTATT
ACGGCCATACCAAGG/CCCACTGG/CCAAACAC
ACCCATCAGG/CCATGG/CC
CCATCCAAGACATTATGCAGGCCTAGACTATTA
CGGCCATACCAAGGCCCACTGGCCAAACAC
ACCCATCAGGCCATGGCC
21/26/8/18/6/2Then color the boxes on the sheet that correspond
*This worksheet shows single stranded DNA
*This restriction enzyme makes a blunt cut
Classwork
*This worksheet shows double stranded DNA as it should be
*This restriction enzyme makes a sticky cut
WorksheetEar Phone DNA
6/23/15/3Then color the boxes on the sheet that correspond
GTCGACCGGTGACCGTGCGTACAGTGCTATCAGCTGGCCACTGGCACGCATGTCACGATA
CCGGATAGCTAATAGCTCCGGTG GGCCTATCGATTATCGAGGCCAC
*This worksheet shows double stranded DNA as it should be
*This restriction enzyme makes a sticky cut
GTCGAC CGGTGACCGTGCGTACAGTGCTATCAGCTGGC CACTGGCACGCATGTCACGATA
C CGGATAGCTAATAGCTC CGGTG GGC CTATCGATTATCGAGGC CAC
*You count the pairs