DNA Nanostructures and Patterning

Taylor StevensonNucleic Acid Engineering

February 15th, 2011

Agenda

• Structures – Origami – Branched Annealing– Nanotubes– Alternative Materials

• Patterning and Application– Combing– Origami as Scaffold– Micropatterning Nanotubes

Origami Structures

Rothemund “Folding DNA to create nano-scale shapes and patterns” Nature Articles (2006)

Origami Structures

Rothemund “Folding DNA to create nano-scale shapes and patterns” Nature Articles (2006)

Branched Annealing Structures

Modified from Luo et.al. Nature Letters 2006 5.

Nano-Tube Structures

Chen et. al. “Approaching The Limit: Can One DNA Oligonucleotide Assemble into Large Nanostructures?” (2006)

Other Forms of Nucleic Acid

Braasch & Corey, Chemistry and Biology (2001)

Other Forms of Nucleic Acid

Lin et. al. “Mirror Image DNA Nanostructures forChiral Supramolecular Assemblies” Nano Letters (2009)

Combing Linear DNA for Nanowires

Deng and Mao “DNA-Templated Fabrication of 1DParallel and 2D Crossed Metallic

Nanowire Arrays” Nano Letters (2003)

DNA Origami as a Scaffold

•RNA/DNA hybridization assays

Ke et. al. “Self-Assembled Water-Soluble Nucleic Acid Probe Tiles for Label-Free RNA Hybridization Assays”

Science (2008)

DNA Origami as a Scaffold

Ke et. al. “Self-Assembled Water-Soluble Nucleic Acid Probe Tiles for Label-Free RNA Hybridization Assays”

Science (2008)

DNA Origami as a Scaffold

Rinker et. al. “Self-assembled DNA nanostructuresfor distance-dependent multivalentligand–protein binding” Nature Letters (2008)

Micropatterning Nanotubes

Lin et. al. “Functional DNA Nanotube Arrays: Bottom-Up Meets Top-Down” Ange Chem (2007)

2D Patterning Branched Annealing Structures

2D Patterning Branched Annealing Structures

• Rigidity of “monomers” affects 2/3D structure.

• Varying the length of red region produces different flexibilities.

Stiffness

Flexibility

2D Patterning Branched Annealing Structures

Stiffness

Flexibility

2D Patterning Branched Annealing Structures

Micropatterning Nanotubes