DNAfromfruit

KomalZahrah,HenrietteMair-Meijers,JustynaResztak,AnthonyFindley,FrancescaLuca

CenterforMolecularMedicineandGenetics

WayneStateUniversityfluca@wayne.edu

Materials•  1Resealable(e.g.Ziplock)bag•  1Bananaor2strawberries(canbe

substitutedbyatomato,kiwi,apple)•  2tspDishdetergent•  ½Cupofwater•  2Clearcups(plasticorglass)•  1Coffeefilterorfinemeshstrainer•  1tspofsalt•  ½Cupofcoldrubbingalcohol(Putbottlein

freezerbeforeuse)•  Coffeestirrerorwoodenskewer•  Measuringspoons

Procedure1.  Takethepeeloffthebanana(oruse2strawberries).2.  Putthefruitintotheplasticbag.3.  Sealthebag.4.  Completelymashthefruitwithyourhands(thisbreaksopenthecellsand

releasestheDNA!).5.  Inacup,maketheDNAextractionliquid:

1.  Mixtogether2teaspoonsofdetergent,1teaspoonofsalt,and½cupofwater

6.  Add2teaspoonsoftheDNAextractionliquidintothebagwiththefruit.7.  Resealthebagandgentlysmash/mixforanotherminute(avoidmakingsoap

bubbles).8.  Placethecoffeefilter/strainerinsidetheothercup.9.  Openthebagandpourthecontentfromthebagintothefilter.10.  Gentlysqueezethefiltertolettheliquidseepthrough.11.  Next,pourdownthesideofthecupanequalamountofcoldrubbingalcohol

asthereisfruitliquidanddonotmixorstir.12.  Withinafewseconds,watchforthedevelopmentofawhitecloudy

substance(DNA)inthetoplayerabovethefruitextractlayer.13.  TiltthecupandpickuptheDNAusingaplasticcoffeestirrerorwooden

stick.14.  YouhavenowisolatedDNAfromfruit!

DNA from Fruit

WhatisDNA?

“It'sahistorybook-anarrativeofthejourneyofourspeciesthroughtime.It'sashopmanual,withanincrediblydetailedblueprintforbuildingeveryhumancell.Andit'satransformativetextbookofmedicine,withinsightsthatwillgivehealthcareprovidersimmensenewpowerstotreat,preventandcuredisease.”-FrancisCollins

Every cell in our body has the same DNA .

Eye cell

Lung cell

Toe cell

Karyotype

How Can We Use This Information?

Better understanding of human disease

Insight into human origins

Personalized medicine & Pharmacogenetics

Identifying genetic susceptibility to disease

Greater insight into cognitive function

WHATCANWELEARNFROMTHEHUMANGENOME?

HowmuchDNAisinonehumancell?

Genome=46(23x2)chromosomes

Genome=approx.3billion

basepairs.Ifstretched=2meters(6.6ft)

DNAsequenceinanytwopeopleis99.9%identical–

only0.1%isunique!HowmuchDNAisinone

strawberrycell?Genome=56(7x8)chromosomes

Genome=approx.960millionbasepairs

“SillyScience”isorganizedbytheLucaLab.

www.lucalab.wayne.edu

Credit

•  Bananapicture:https://upload.wikimedia.org/wikipedia/commons/thumb/f/f7/Bananas.svg/560px-Bananas.svg.png

•  Strawberrypicture:•  https://pngimg.com/uploads/strawberry/strawberry_PNG2586.png

•  Dnapicture:https://pngimg.com/uploads/dna/dna_PNG15.png

•  Adaptedfrom:https://www.genome.gov/Pages/Education/Modules/StrawberryExtractionInstructions.pdf