*DNA Fingerprinting**DNA Fingerprinting*Glue notes into pg 51Glue notes into pg 51Glue paternity tests Glue paternity tests

sheet into pg 50sheet into pg 50

Background InfoBackground Info

People who have different characteristics People who have different characteristics also have different DNA sequencesalso have different DNA sequences– Ex. Person #1 ATTCCGATEx. Person #1 ATTCCGAT– Person #2 TCGGCTATPerson #2 TCGGCTAT

The more variation in features, the more The more variation in features, the more variation in DNA sequencesvariation in DNA sequences

We have learned to identify these We have learned to identify these sequencessequences

UsesUses Diagnosis of inherited disorders in all agesDiagnosis of inherited disorders in all ages Paternity suitsPaternity suits Identification of bodiesIdentification of bodies Crime investigationsCrime investigations

Method Method 1. Extract the DNA 1. Extract the DNA

– from cells (blood, skin, hair, cheek)from cells (blood, skin, hair, cheek)– only small amount neededonly small amount needed– same pattern found in every cell in an organismsame pattern found in every cell in an organism

2. Cutting the DNA2. Cutting the DNA– use use restriction enzymes restriction enzymes to cut at a to cut at a specificspecific

placeplace– ex. EcoR1 cuts when it see’s GAATTCex. EcoR1 cuts when it see’s GAATTC

Cont.Cont. 3. Sorting the DNA3. Sorting the DNA

– DNA pieces are sorted electricallyDNA pieces are sorted electrically

according to size using the process of according to size using the process of gel gel electrophoresiselectrophoresis

4. Staining4. Staining– Add radioactive/colored dye so it can be seenAdd radioactive/colored dye so it can be seen

DNA FingerprintDNA Fingerprint

Larger pieces

Smaller Pieces

Crime SceneCrime Scene

Paternity CasesPaternity CasesEvery band the child has must match up with either mom or dad. If they don’t the guy is NOT THE FATHER