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• DNA and RNA are large macromolecules with several levels of complexity
• Nucleotides form the repeating units
• Phosphodiester bonds link nucleotides to form a strand
• Two strands interact to form a double helix
• The double helix interacts withproteins resulting in 3-D structuresin the form of chromatin
NUCLEIC ACID STRUCTURE
3D structure
9-25Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or displayFigure 9.8
Nucleotide Components
9-27Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
• Base + sugar nucleoside – Example
• Adenine + ribose = Adenosine• Adenine + deoxyribose = Deoxyadenosine
• Base + sugar + phosphate(s) nucleotide– Example
• Adenosine monophosphate (AMP)• Adenosine diphosphate (ADP)• Adenosine triphosphate (ATP)
Combining all the parts
9-28Figure 9.10
Base always attached here
Phosphates are attached there
9-26
Figure 9.9 The structure of nucleotides found in (a) DNA and (b) RNA
A, G, C or T A, G, C or U
dNMP NMP
Nucleotide Polymerization Reaction: Phosphodiester Bond Formation
9-30Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or displayFigure 9.11
9-31Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
• 1953, James Watson and Francis Crick discovered the double helical structure of DNA
• The scientific framework for their breakthrough was provided by other scientists including– Linus Pauling– Rosalind Franklin– Erwin Chargaff
Events Leading to DNA Structure Determination
Linus Pauling
Rosalind Franklin• Helical• Double stranded• 10 base pairs per turn
X-ray Diffraction Pattern of DNA
9-35Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
• It was assumed the four bases: A, G, C and T were in a repeating, tetranucleotide configuration
• Therefore, there should be the same amount of A, G, C & T in any molecule of DNA form any source
• Chargaff carefully determined the exact percentages of nuceotides in DNA from several sources
Erwin Chargaff’s Experiment
Erwin Chargaff’s Data
9-39
• % A = % T & %G = %C• However %AT DID NOT = %GC• This observation became known as Chargaff’s rule
Watson & Crick Model DNA Structure
Base Pairing Key to DNA Structure
Features of the DNA Double Helix
NH2
T
2 nm
One nucleotide0.34 nm
One completeturn 3.4 nm
O
O
HH
NN
H
H
HHOOO
OPO–
NH2
HN
N
NH
N
HH
H
OH
HHOOO
O–
PO–
HH
H
HHOOO
OPO–
NH2
O
O
H H
NN
H2N
HN
N
NH
N
H H
H
HHO
OOO–
PO
H H
H
HHO OO
O–
PO
H N
NH
N
N
H H
H
HHO
OOO–
PO–
H2N
HO5end 3end
3hydroxyl3end 5 end
5phosphate
TA
T A
T AP
PP
P
P
S
S
S
S
SS
S
S
A
C G
G
C G
C G
G
G
C
C
G C
GC
G C
C
P
PS
P
PP
P
P
S
SS
S
S
PP
P
P
PP
P
P
S
S SS
S
S
S
S
SP
S
S
P
PP
S
SS
SP
CH2
CH2
CH2
H2N
CH2
CH2
CH2
A
P
S
P
P
H
CH3H
NNHO
O
Space-filling model of DNA
Ball-and-stick model of DNA
Minorgroove
Majorgroove
Minorgroove
Majorgroove
Features of the DNA Double Helix
Major Helical Conformations of DNA
A-DNA B-DNA
A26
0
Melting Point Curve: Tm is Proportional to %GC
Tm= 68.9 + (0.41)(%GC)
9-55
The Three-Dimensional Structure of DNA
Figure 9.21
9-57
• The primary structure of an RNA strand is much like that of a DNA strand
• RNA is made as a single strand only, however it may form a double stranded structures
• RNA strands can be a 10s to1000s of nt in length
• RNA is made from a DNA template - only one of the two strands of a DNA helix is used as the template
• RNA contains uracil rather than thymine
RNA Structure
9-58Figure 9.22
9-60
Figure 9.23
RNA Secondary Structures
9-61
Figure 9.24
RNA Tertiary Structure – a tRNA