Distributor GE Healthcare BioXtra 2018

Distributor GE Healthcare

BioXtra™ 2018Transforming your science to generate results.

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Table of ContentsIsolation and cloningSample prep and isolation of nucleic acids Whatman™ Non-Indicating FTA™ classic cards . . . . . . . . 4

Sera-Mag™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

PCR – Cloning illustra™ Hot Start Mix RTG™ . . . . . . . . . . . . . . . . . . . . . . . . . . 5 illustra GenomiPhi™ V2 DNA amplification kits . . . . . . . . 5

Amplification using illustra GenomiPhi Kits . . . . . . . . . .6

Cloning – Purification – Analysis illustra GFX™ PCR DNA and Gel Band Purification Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 illustra TempliPhi™ 500 Amplification Kit . . . . . . . . . . . . . . 7

Plant genomics Nucleon PhytoPure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 FTA PlantSaver Card . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Sera-Mag and Sera-Mag SpeedBeads™ . . . . . . . . . . . . . . . . 9

Cell CultureHyClone™ Specialty Serum . . . . . . . . . . . . . . . . . . . . . . . . . .10

Specialty FBS products Charcoal/Dextran Treated FBS . . . . . . . . . . . . . . . . . . . . . . . . 10 Dialyzed FBS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 Super Low IgG FBS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Screened FBS products HyClone tetracycline screened Fetal Bovine Serum (FBS), U.S. origin . . . . . . . . . . . . . . . . . . 11 Human Mesenchymal Stem Cell Screened FBS . . . . . . . . 11 ES Cell Screened FBS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Insect Cell Screened FBS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Classic liquid media HyClone HyQ™-RS reduced serum media . . . . . . . . . . . . . 12 HyClone Minimum Essential Medium . . . . . . . . . . . . . . . . . 12

Research essentials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13Process liquids, buffers and salt solutions . . . . . . . . . . . .13Supplements, buffering agents and antibiotics . . . . . . .13

Sample PrepWhatman syringe filters Whatman Uniflo™ Syringe Filters . . . . . . . . . . . . . . . . . . . . . 14 Whatman GD/X™ Sterile Syringe Filter . . . . . . . . . . . . . . . . 14

Analytical sample prep simplified Whatman Mini-UniPrep™ (MUP) Syringeless Filters . . . . 15

Chemical compatibility of membranes and housings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16

PurificationProtein purification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18

ÄKTA™ start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19

Affinity chromatography – Tagged proteins HiTrap™ TALON® crude columns . . . . . . . . . . . . . . . . . . . . . . 20 Glutathione Sepharose™ 4 Fast flow resin . . . . . . . . . . . . . 20 His Mag Sepharose Ni beads . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Affinity chromatography – Antibodies HiTrap MabSelect™ PrismA protein A column . . . . . . . . . 21

Ion exchange chromatography HiTrap Capto™ Q anion exchange chromatography column. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Size exclusion chromatography columns . . . . . . . . . . . . .22

New generation SEC columns packed with Superdex™ Increase and Superose™ Increase resins . . . 22

Desalting and buffer exchange HiPrep™ desalting columns . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Protein concentration Vivaspin™ concentrators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Your guide to chromatography resins . . . . . . . . . . . . . . . . .24

AnalysisWestern blotting detection ECL™ Western Blotting Detection Reagents . . . . . . . . . . 26

Western blotting membranes . . . . . . . . . . . . . . . . . . . . . . . .27

Western blotting essentials Amersham™ ECL Mouse IgG, HRP-linked whole Antibody (from sheep) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 Protein A Mag Sepharose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Lab essentials VACU-GUARD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Benchkote™ surface protector . . . . . . . . . . . . . . . . . . . . . . . . 29

For more information please visit vwr .com/gehealthcare

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Isolation& Cloning

CellCulture

SamplePrep

Purification

Analysis

Protein Workflow Simplified

ISOLATION AND CLONING

Sample prep and isolation of nucleic acids

DNASource

DNAAmpli�cation

DNAPuri�cation

Construct& Transform

Ampli�cation

Expression Puri�cation

Sequencing

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Whatman Non-Indicating FTA Classic Cards Collect, stabilize, process, transport, and archive blood samples with Whatman Non-Indicating FTA Classic Cards.

• Immediate stabilization and protection of nucleic acids

• Long-term archiving of samples at room temperature

Sera-MagCarboxylate-Modified Magnetic ParticlesSera-Mag Carboxylate-Modified Magnetic Particles allow convenient covalent coupling of target molecules.

• Easy covalent coupling to biomolecules of interest using convenient carbodiimide chemistry

• Excellent sensitivity and low nonspecific binding for greater accuracy

Description Ordering Code Ordering Quantity

FTA Classic 10035-868P 25/pk

FTA Classic 14222-800P 100/pk

FTA Microcard 14222-808P 100/pk

FTA Minicard 14222-804P 100/pk

FTA PlantSaver 80077-200P 100/pk

Multi-barrier pouches, 11x16.5cm 89048-950P 100/pk

Multi-barrier pouches, 9.5x7.5cm 89008-898P 100/pk

Desiccant 1g 14222-840P 1000/pk

Uni-Core punch 1mm 30621-462P 25/ea

Cutting Matt 6.0x8.0in 14222-832P 1/pk

903 PROTEINSAVER Card 28170-017P 100/pk

PLASTIC ZIPLOC® BAG 89027-022P 100/pk

Sera Magnetic Beads

Sera-Mag magnetic carboxylate modified particles, 1µm, 5% solids, 15mL, 0.05% Na azide.

10204-628P 15mL

Sera-Mag Magnetic Oligo (dT) particles, 1µm, 1% solids, 1mL, 0.05% Na azide 10204-656P 1mL

Encapsulation

Magnetite

Core

PCR – Cloning

DNASource

DNAAmpli�cation

DNAPuri�cation


Ampli�cation


Sequencing

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illustra Hot Start Mix RTG Preformulated and predispensed, freeze-dried PCR reagents mix including high-quality PuReTaq DNA Polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications

• Avoids the risk of contamination from a mammalian source associated with hot start antibodies

• Streamlines the workflow and yields more reproducible results

illustra GenomiPhi V2 DNA Amplification Kitsillustra GenomiPhi V2 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 4 to 7 µg yield from nanogram amounts of DNA sample.

• No template-independent, background amplification product

• Quick and simple automation-friendly protocol; no thermal cycler required


illustra RNAspin Mini Kit 95017-491P 50/pk

First Strand cDNA synthesis Kit 95040-330P 55 reactions

Hot Start RTG RT PCR 89497-140P 96 reactions

GenomiPhi V2 95040-352P 100 reactions

GenomiPhi HY Kit 95042-268P 25 reactions

Lab Essentials

VACU-GUARD 60mm 0.45µm PTFE 28137-737P 10/pk

Litmus paper blue, 7mmx5m 14217-684P 1 reel

105 10x15cm Lens tissue paper 97002-542P 25/pk

Benchkote sheets 46x47cm 52855-103P 50/pk

Simply add your sample and primer

Amplification using illustra GenomiPhi Kits

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DNASource

DNAAmpli�cation

DNAPuri�cation


Ampli�cation


Sequencing

What type of DNA do you want to amplify?

Circular/plasmid DNAGenomic DNA

Liquid format(requires freezer storage)

Amplification from 1–1000 cells

illustra Single cell GenomiPhi

Amplification from > 1 ng genomic DNA

Standard yield (up to 20 µg)

illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kits

High yield (up to 60 µg)illustra Ready-To-Go

GenomiPhi HY DNA Amplification Kits

Standard yield (up to 10 µg)

illustra GenomiPhi™ V2 DNA Amplification Kits

Higher yield (up to 50 µg)illustra illustra GenomiPhi HY

DNA Amplification Kits

Ready-To-Go™ formatPre-dispensed, room

tempeature stable

Large DNA > 30 kb, e .g . fosmids, BACs

illustra TempliPhi Large Construct Kit

GC-rich DNAillustra TempliPhi

Sequence Resolver Kit

DNA < 30 kb, e .g . plasmids

illustra Templihi™ DNA Amplification Kits

Schematic diagram of the amplification process with the GenomiPhi amplification method. Random hexamer primers anneal to the template DNA at multiple sites. Phi29 DNA polymerase initiates replication at multiple sites on the denatured linear DNA simultaneously. As synthesis proceeds, strand displacement of complementary DNA generates new single-stranded DNA. The subsequent priming and strand displacement replication of this DNA results in the formation of double-stranded DNA.

Phi29 DNA polymerase

Random hexamer primers

Input DNA

dNTPs

Cloning – Purification - Analysis

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DNASource

DNAAmpli�cation

DNAPuri�cation


Ampli�cation


Sequencing

illustra GFX PCR DNA and Gel Band Purification Kit Isolation and concentration of DNA fragments quickly from:

• PCR mixtures

• Agarose gel bands

• Enzyme digests

DNA for use in sequencing, PCR, labeling, digestion, and cloning

illustra TempliPhi 500 Amplification KitIsolation and concentration of DNA fragments quickly from:

• PCR mixtures

• Agarose gel bands

• Enzyme digests

DNA for use in sequencing, PCR, labeling, digestion, and cloning


Cloning and Amplification

GFX PCR Gel Band Purification Kit 95026-728P 100 Purifications

illustra Plasmid Mini Kit 95026-750P 250 Reactions

TempliPhi Amplification Kit 89131-612P 500 Reactions

Nucleic Acid Blotting

Rapid Hyb Buffer 95038-160P 500mL

Hybond™ N+, 300cmx3m 95038-400P 1 Roll

Hybond-N, 20cmx3m 95038-382P 1 Ea

Alkphos direct labelling module 95038-288P (For 2500cm2 membrane) 1Ea

ECL Direct nucleic acid labeling and detection system 95038-276P (For 2500cm2 membrane) 1Ea

Plant genomics

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DNASource

DNAAmpli�cation

DNAPuri�cation


Ampli�cation


Sequencing

Nucleon PhytoPureQuality, length, and purity of a phenol-chloroform extraction without phenol. Nucleon PhytoPure enables rapid chloroform extraction of high-quality, high molecular weight genomic DNA from plant and fungal samples with efficient removal of protein as well as polysaccharides.

• Ensures high yields of high-quality plant genomic DNA in only 1 h

• Successfully extracts DNA from many plant species, starting with fresh, frozen, or freeze dried material

FTA PlantSaver CardFTA PlantSaver card with four sample areas per card

• Capture nucleic acid in one easy step

• Captured nucleic acid is ready for downstream applications in less than 30 min

• DNA collected on FTA Cards is stable for years at room temperature


Plant Genomics

FTA PlantSaver 80077-200P 100/pk

Nucleon PhytoPure 1.0g 95026-704P 50 preps/1.0g

Hot Start RTG RT PCR 89497-140P 96 reactions

GFX PCR Gel Band Purification Kit 95026-728P 100 purifications

Lab Essentials


105 10x15cm Lens tissue 97002-542P 500/pk


Sera-Mag and Sera-MagSpeed Beads

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Sera Mag and Sera Mag SpeedBeadsSera-Mag and Sera-Mag SpeedBeads provide cost effective magnetic bead separation technology for molecular biology applications, nucleic acid isolation, and research immunoassays. Sera-Mag superparamagnetic particles combine a fast magnetic response time with a high binding capacity. The particles feature a large surface area, high sensitivity, physical stability, and fast reaction kinetics. Carboxylatemodified, Streptavidin- and Oligo(dT)-coated versions are available.

Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles feature carboxylic groups on the surface that permit easy covalent coupling using simple carbodiimide chemistry.

Encapsulation

Magnetite

Core


Sera-Mag magnetic carboxylate modified particles, 1 um, 5% solids, 15 mL, 0.05% Na azide. 10204-628P 15mL

Sera-Mag Magnetic Olido(dt) particles, 1 um, 1% solids, 1 mL, 0.05% Na azide 10204-656P 1mL

NHS Mag Sepharose 89129-100P 4x500uL

His Mag Sepharose Ni 89166-170P 2x1mL

His Mag Sepharose Ni 89166-172P 5xmL

MagRack Maxi 97060-912P 1Ea

Sera-Mag SpeedBead Protein A/G particles, 1 um, 1% solids, 1 mL, 0.05% Na azide. 10204-752P 1mL

Protein A Mag Sepharose 89129-104P 4x500µL

Protein G Mag Sepharose 89129-102P 4x500µL

Mag Rack 6 89129-096P 1Ea

Polystyrene Core First Layer of magnetite Layer of magnetite locked in by polymer encapsulation

Sera-Mag Magnetic Particles feature a single layer of magnetite but with the same cauliflower-like surface of the SpeedBeads.

Second layer of magnetite with carboxylated polymer surface

Encapsulation

Magnetite

Core

Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particle

CELL CULTURE

Animal serum origins Serum origin refers to the country in which the raw blood was collected, and is not to be confused with the country in which the finished product was processed. With HyClone serum products, the country or countries of origin are stated in the certificate of analysis that you receive with your product.The supply of FBS, a byproduct of the beef industry, is impacted by complex market conditions. For these reasons, we ensure adequate supply for our customers by not only sourcing FBS from several regions, but also working with a broad base of serum and raw blood suppliers. To ensure traceability and security of supply, raw serum is obtained in New Zealand, Australia, the United States, Canada, Central America, and South America. Moreover, we are traceability certified by the International Serum Industry Association (ISIA) and send samples of characterized and defined FBS to Oritain for independent testing.

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Description and origin Filtration En

doto

xin

(EU

/mL)

Hem

oglo

bin

(mg/

dL)

Osm

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(mO

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g)

Tota

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(gm

/dL)

Ster

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bac

teria

and

fung

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Fluo

resc

ent a

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test

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Cyto

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Hem

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Myc

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Prot

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Trac

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Elec

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IgG

(µg/

mL)

Specialty fetal bovine serumSuper Low IgG FBS, US 40 nm ≤ 10 ≤ 10 • • • • • • • • • • • ≤ 5

Charcoal/Dextran Treated FBS, US Triple 100 nm ≤ 10 ≤ 20 • • • • • • • • • • • •

Dialyzed FBS, US Triple 100 nm ≤ 10 ≤ 10 • • • • • • • • • • • •

ES Cell Screened FBS, US 40 nm ≤ 10 ≤ 10 • • • • • • • • • • • •

Tetracycline Screened FBS, US 40 nm ≤ 10 ≤ 10 • • • • • • • • • • • •

Insect Cell Screened FBS, US 40 nm ≤ 10 ≤ 10 • • • • • • • • • • • •

Human Mesenchymal SC

Screened FBS40 nm ≤ 10 ≤ 10 • • • • • • • • • • • •

FIO = for information only

• = Tested. For typical results, request a certificate of analysis or go to www.gelifesciences.com/HyClone

Industry leading traceability program gives peace of mind

In addition, we go beyond industry standards of document traceability by performing independent testing by Oritain™ on each batch of characterized and defined FBS from the United States, Australia and New Zealand. Look out for the Oritain logo on your FBS bottle.

Origin of sera is important. As proof that we ensure proper origin, traceability, and truth in labeling, we are traceability certified by the International Serum Industry Association (ISIA).

HyClone Specialty Serum

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Specialty FBS productsOur specialty FBS products augment cell culture growth and support several applications that might require lower IgG levels, reduced levels of various hormones, or reduced concentrations of nucleotides and amino acids.Charcoal/Dextran Treated FBS• Proprietary processing to reduce levels of various hormones and growth factorsDialyzed FBS• Processed using proprietary diafiltration method • Depleted from small molecules (less than Mr 10 000)Super Low IgG FBS• Reduce IgG levels to less than 5 µg/mL, while still retaining excellent cell growth properties• For use in monoclonal antibody production, virus propagation, and immunoassay procedures

Screened FBS productsPrescreened from 40 nm filtered Defined FBS, our specialty serum products meet the needs of many specific cell culture applicationsHyClone tetracycline screened Fetal Bovine Serum (FBS), U .S . origin• FBS with undetectable levels of tetracyclineHuman Mesenchymal Stem Cell Screened FBS• Designed for human mesenchymal stem cells ES Cell Screened FBS • Optimized for the culture of murine embryonic stem cellsInsect Cell Screened FBS• To optimize performance of insect cells


Specialty Serum

Charcoal/Dextran treated FBS, US origin 16777-228P (500mL) 1Ea

Dialyzed FBS, US origin 16777-212P (500mL) 1Ea

Super low IgG FBS, US origin 95042-944P (500mL) 1Ea

Tetracycline-screened FBS, US origin 82007-422P (500mL) 1Ea

Screened Serum

Embryonic stem cell-screened FBS, US origin 43300-310P (500mL) 1Ea

Human Mesenchymal stem cell-screened FBS, US origin 95059-626P (500mL) 1Ea

Insect cell-screened FBS, US origin 82007-420P (500mL) 1Ea

Lab Essentials


Litmus paper blue 7mmx5m 14217-684P 1 reel



SAVE UP TO 10%

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Classic liquid mediaOur extensive line of classical media is system-tested with HyClone serum to ensure product efficacy and homogeneity.

HyClone HyQ-RS reduced serum mediaHyClone HyQ-RS media DMEM-RS and MEM-RS are reduced serum (RS) versions of Dulbecco’s Modified Eagle Medium (DMEM) and Minimal Essential Medium (MEM), respectively

HyClone Minimum Essential MediumSupport cell culture growth and biomanufacturing processes with GE Healthcare Life Sciences HyClone Minimum Essential Medium (MEM) with Earle's Balanced Salts (MEM/EBSS), manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities.

Certificates of Analysis available on GE Healthcare Life Sciences website


Classic Media

DMEM-RS (reduced serum) 82017-966P 500mL

MEM-RS (reduced serum) with glutamine, EBSS 82017-970P 500mL

DMEM with High glucose, L-glutamine, Sodium pyruvate 16750-074P 6x500mL/pk

DMEM with Low glucose, L-glutamine, Sodium pyruvate 16750-094P 6x500mL/pk

MEM with EBSS, L-glutamine 16750-080P 6x500mL/pk

RPMI 1640 with L-glutamine 16750-070P 6x500mL/pk

RPMI 1640 without L-glutamine 16750-084P 6x500mL/pk

Lab Essentials





SAVE UP TO 10%

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Research essentials Complete solution for all your basic research needs from our HyClone cell culture portfolio

Process liquids, buffers and salt solutionsComplements a broad range of solutions for cell culture and bioprocessing including, hydration, viral reduction, chromatography, cleaning, and sanitation

Supplements, buffering agents and antibioticsIncrease cell culture yields by using supplements, buffer cell culture with a fully QC-tested solution, and control microorganism contamination with a variety of antibiotics


Process Liquids, Buffer and Salt Solutions

WFI Quality Water 16750-108P 6x1000mL/pk

Cell Culture Grade Water 16750-114P 6x1000mL/pk

Molecular Biology Grade Water 16750-128P 6x1000mL/pk

DPBS, 1X, without calcium, magnesium, phenol red 16750-076P 6x1000mL/pk

HBSS, 1X, without calcium, magnesium, with phenol red 16750-092P 6x1000mL/pk

PBS, 1X, without calcium, magnesium, with phenol red 16750-078P 6x1000mL/pk

Cell Culture Essentials

AdvanceSTEM™ ES Qualified HEPES Solution 95053-212P 100mL

Sodium bicarbonate 16777-160P 100mL

Trypan blue solution 82024-256P 100mL

Antibiotic antimycotic solution (Pen/Strep/Fungizone), 100X 82026-730P 100mL

Pen/Strep/Glutamine solution 16777-169P 100mL

Penicillin/Streptomycin solution 16777-164P 100mL

Lab Essentials





SAVE UP TO 40%

HyClone Water for Injection (WFI) Quality Water

HyClone Penicillin/Streptomycin/Glutamine solution

SAMPLE PREP

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Item Description Ordering Code Ordering QuantityUniflo Syringe Filters Whatman Uniflo 13mm 0.22 PES 75849-380P 500/pkWhatman Uniflo 13mm 0.45 PES 75849-382P 500/pkWhatman Uniflo 25mm 0.22 PVDF 75849-360P 500/pkWhatman Uniflo 25mm 0.45 PVDF 75849-362P 500/pkWhatman Uniflo 25mm 0.22 PTFE 75849-376P 500/pkWhatman Uniflo 25mm 0.45 PTFE 75849-378P 500/pkWhatman Uniflo 25mm 0.22 PES 75849-384P 500/pkWhatman Uniflo 25mm 0.45 PES 75849-386P 500/pkUniflo Syringe Filter Sterile Whatman Uniflo 25mm 0.22 PVDF Sterile 76211-202P 45/pkWhatman Uniflo 25mm 0.45 PVDF Sterile 76211-004P 45/pkWhatman Uniflo 25mm 0.2 PES Sterile 76211-006P 45/pkWhatman Uniflo 25mm 0.45 PES Sterile 76211-008P 45/pkWhatman Uniflo 25MM 0.2 PES Sterile 76211-010P 200/pkWhatman Uniflo 25MM 0.45 PES Sterile 76211-012P 200/pkWhatman Uniflo 13mm 0.2 PES Sterile 76211-014P 100/pkWhatman Uniflo 13mm 0.45 PES Sterile 76211-016P 100/pkGD/X Syringe Filters GD/X 25/0.2 PES Sterile 28138-166P 150/pkGD/X 25/0.45 PES Sterile 28138-168P 150/pkLab Essentials VACU-GUARD 60mm 0.45µm PTFE 28137-737P 10/pkLitmus paper blue 7mmx5m 14217-684P 1 reel105 10x15cm Lens tissue paper 97002-542P 25/pkBenchkote sheets 46x47cm 52855-103P 50/pk

SAVE UP TO 25%

Whatman Uniflo Syringe FiltersConsistent performance, maximum valueWhatman Uniflo Syringe Filters are disposable filter units designed to provide clean filtrate from small volumes up to 100 mL. They are available in a variety of membrane choices with a polypropylene overmold housing, and include both sterile and non-sterile options.

Whatman GD/X Sterile Syringe FilterGE’s Whatman GD/X sterile syringe filter is designed to support filtration of viscous, hard-to-filter samples with high solids content.

• Reduces need for multiple syringe filters by processing three-to-seven times more sample volume

• Reduces the need to carry out separate prefiltration step for difficult-to-filter samples

Whatman Syringe Filters

NEW Sterile Uniflo

Analytical Sample Prep Simplified

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SAVE UP TO 25%

Whatman Mini-UniPrep (MUP) Syringeless FiltersGE’s Whatman Mini-UniPrep Syringeless Filters integrate an autosampler vial, filtration membrane, plunger, and cap/septa into one consumable product. They are built for fast and easy HPLC/UHPLC sample preparation.

• All-in-one filtration device for quick and cost-effective sample processing

• Eliminates the need for separate syringes, syringe membrane filters, vials, and septa

• Designed for use with an HPLC or UHPLC autosampler for high-throughput analysis

Item Description Ordering Code Ordering QuantitySyringeless Filter MUP 0.2µm RC 97015-564P 100/pkMUP 0.45µm RC 97015-562P 100/pkMUP 0.45µm GMF 28137-770P 100/pkMUP Amber 0.2µm PES 84009-510P 100/pkLab Essentials VACU-GUARD 60mm 0.45µm PTFE 28137-737P 10/pkLitmus paper blue 7mmx5m 14217-684P 1 reel105 10x15cm Lens tissue paper 97002-542P 25/pkBenchkote sheets 46x47cm 52855-103P 50/pk

Mini-UniPrep filter vial replaces syringe filter, syringe, autosampler vial, cap, and septum.

Max. unfiltered capacity 400 µlMax. Filtered capacity: 350 µlDead volume: 50 µl

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Chemical compatibility of membranes and housingsSolvent ANP CA CN PC PE GMF NYL PP DpPP PES PTFE** PVDF RC

Acetic acid, 5% R LR R R R R R R R R R R

Acetic acid, glacial R NR NR R LR R R R R R NR

Acetone R NR NR NR R R R R R NR R NR R

Acetonitrile R NR NR R R R R NR R R R

Ammonia, 6N NR NR NR LR LR R R R R R LR LR

Amyl acetate LR NR NR NR R R R R R LR R LR R

Amyl alcohol R LR LR R R R R NR R R R

Benzene* R R R NR R R LR NR NR R R R R

Benzyl alcohol* R LR LR LR R R LR R R NR R R R

Boric acid R R R R R R LR R R R R R

Butyl alcohol R R R R R R R R R R R R R

Butyl chloride* R NR NR NR R R

Carbon tetrachloride* R NR R LR R R LR NR NR NR R R R

Chloroform* R NR R NR R R NR LR LR NR R R R

Chlorobenzene* R LR NR R NR LR NR R R R

Citric acid R LR R R R R R

Cresol* NR R R NR NR NR NR R NR R

Cyclohexanone R NR NR R NR R R NR R R R

Cyclohexane* R NR NR R R R NR NR NR NR R R R

Diethyl acetamide NR NR R R R R R NR R

Dimethyl formamide LR NR NR R R R R NR R NR LR

Dioxane R NR NR NR R R R R R LR R LR R

DMSO LR NR NR NR R R R R R NR R LR LR

Ethanol R R NR R R R R R R R R R R

Ethers* R LR LR R R R R NR NR R R LR R

Ethyl acetate R NR NR NR R R R R R NR R NR R

Ethylene glycol R LR LR R R R R R R R R R R

Formaldehyde LR LR R R R R R LR LR R R R LR

Freon TF* R R R R R R NR NR NR R R R

Formic acid LR LR R NR R R R R R LR

Hexane R R R R R R R R R R R R R

Hydrochloric acid, conc* NR NR NR NR NR R NR LR LR R R R NR

Hydrofluoric acid* NR NR NR NR LR LR R R NR

Isobutyl alcohol R LR LR R R R R R R R R R

Isopropyl alcohol R R LR R R R R R R R

Methanol R R NR R R R R R R R R R R

Methyl ethyl ketone R LR NR NR R R R R R NR R NR R

Methylene chloride* R NR LR R NR LR LR NR R R R

Nitric acid, conc* NR NR LR NR R NR NR NR NR R R NR

Nitric acid, 6N* LR LR R NR LR LR LR R R LR

Nitrobenzene* LR NR NR NR R R LR R R NR R R R

Pentane* R R R R R R R NR NR R R R R

Perchloro ethylene* R R R R LR NR NR NR R R R

Phenol 0.5% LR LR R R NR R R NR R R R

Pyridine R NR NR NR R R LR R R NR R NR R

Sodium hydroxide, 6N NR NR NR NR NR NR LR R R R R NR NR

Sulfuric acid, conc* NR NR NR NR NR R NR NR NR NR R NR NR

Tetrahydrofuran* R NR NR R R LR LR NR R R R

Toluene* R LR R NR R R LR LR LR NR R R R

Trichloroethane* R NR LR NR R R LR LR LR NR R R R

Trichloroethylene* R R R NR LR LR NR R R R

Water R R R R R R R R R R R R R

Xylene* R R R R LR LR LR LR R R R

R = Resistant; LR = Limited Resistance; NR = Not Recommended

* Short-term resistance of housingThe above data is to be used as a guide only. Testing prior to application is recommended.** Membrane may need pre-wetting with

isopropanol/methanol if filtering a polar liquid

Material abbreviations: ANP – AnoporeCA – Cellulose AcetateCN – Cellulose NitrateDpPP – Polypropylene Depth FilterGMF – Glass MicrofiberNYL – Nylon

PC – PolycarbonatePE – PolyesterPES – PolyethersulfonePP – PolypropylenePTFE – PolytetrafluoroethylenePVDF – Polyvinylidene DifluorideRC – Regenerated Cellulose

17

The Testament to QualityThere can be few companies anywhere in the world can lay claim to such distinguished clients as Whatman in the past 250 years.

In November, 1767, the artistic Thomas Gainsborough wrote; “I beg you to accept my sincerest thanks for the favour you have done me concerning the paper for drawings. I had set my heart upon getting some of it, as it is so completely what I have long been in search of… Upon my honor I would give a guinea a quire for a dozen quires of it. “

Whatman paper became enormously popular with leading artists such as J M W Turner, and the quality and durability of English papers, including those made by Whatman, gave the English watercolour school a 50 year advantage over European artists. At the end of the 18th century, the erratic genius William Blake used Whatman papers for four of his illuminated books, the public being informed that these were printed on “the most beautiful wove paper that could be procured.”

Throughout history, heads of state and world leaders have shown a particular penchant for Whatman paper. Napoleon sat for five hours on the bleak island of St. Helena writing his long and detailed will on Whatman paper only three weeks before his death in 1821. George Washington signed many state documents on Whatman paper. Queen Victoria chose Whatman paper for her personal correspondence.

In the 1930’s Soviet leaders used Whatman paper to published the 5 year plan for the future of the USSR, while the peace treaty with Japan was signed on Whatman paper at the close of World War II.

Today, Whatman filters have gained universal acceptance among the major scientific and industrial concerns of the developed world, from Japan to the United States, the UK to southeast Asia.

A remarkable testimony to Whatman quality – achieved through the pursuit of excellence.*

PURIFICATION

18

Protein purification Protein purification methods can be organized as a single or multi-step chromatographic strategies to optimize the required purity and yield. A strategy can comprise capture(C), intermediate purification (iP), and polishing steps (P).

• Capture or affinity chromatography is where the target protein of interest is isolated, concentrated, and stabilized.

• Intermediate chromatography is used to remove contaminants with techniques including IEX, CIEX, and HIC.

• Polishing is utilized to remove the most difficult impurities and aggregates of the target protein to increase purity.

Depending on your downstream analysis technique, a single or multi-step chromatographic process can be followed by sample desalting, buffer exchange, and concentration.

CaptureIsolate, concentrate, stabilize

Intermediate purificationRemove bulk impurities

PolishingRemove final contaminants

Moderate to high purity

High yield

Step 1

Very high purity Good balance

purity/yield

Step 1

Step 2

Highest purity Moderate yield

Step 1

Step 2

Step 3

19

Item Description Ordering Code Ordering QuantityÄKTA start – Instrument 10142-746P 1 EaFrac30 used in ÄKTA start 10142-748P 1 EaUNICORN™ start 1.1 ESD + eLicence 75844-964P 1 Ea

ÄKTA™ startLeave behind the hassle of time-consuming manual protein purification.

ConvenientEasy transition from manual to automatic purification

Gain deeper insightsGain valuable insights from real-time monitoring and control-software.

Simplify your workPurify tagged proteins and antibodies easily using prepacked columns.

User friendlyEasy-to-use touchscreen display allows you to start the run at the touch of a button.

SAVE UP TO 10%

2020

Affinity chromatography – Tagged proteinsAffinity chromatography step enables the isolation of target tagged protein from initial sample (e.g., lysate, cell culture). After this step, the purity level is > 80%.

If a higher purity level is required, then it is recommended to add extra steps, such as IEX and/or SEC. See pages 24 and 25

Featuring:

HiTrap™ TALON® Crude columnsHiTrap TALON® crude 1 mL and 5 mL columns for rapid separations histidine tagged proteins and easy scale-up with a minimum of sample preparation and equipment. Convenient columns prepacked with cobalt-based resins.

Glutathione Sepharose™ 4 Fast SlowGlutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins.

His Mag Sepharose Ni His Mag Sepharose Ni are magnetic beads designed to simplify small-scale purification and expression screening of histidine-tagged proteins

Item Description Ordering Code Ordering QuantityAffinity Chromatography Columns and Resins HiTrap TALON crude 71006-000P 5x5mL/pkTALON Superflow 71006-008P 50mL/eaGlutathione Sepharose 4 FF 95017-174P 100mL/eaNi Sepharose HP 95056-210P 100mL/eaHIS GraviTrap™ 95056-288P 10x1mL/pkHisTrap™ excel 89233-414P 5x5mL/pkGSTrap™ HP 95056-020P 5x5mL/pkHisTrap FF crude 95056-136P 5x5mL/pkMagnetic Bead Protein Isolation NHS Mag Sepharose 89129-100P 4x500µLHis Mag Sepharose Ni 89166-170P 2x1mLHis Mag Sepharose Ni 89166-172P 5x1mLMagRack Maxi 97060-912P 1 pieceSera-Mag SpeedBead Protein A/G particles, 1µm, 1% solids, 1mL, 0.05% Na azide.

10204-752P 1mL

SAVE UP TO 15%

21

Affinity chromatography – AntibodiesAffinity chromatography step enables the isolation of target anitbody from initial sample (e.g., lysate, cell culture). The purity obtained after this step is usually high (above 90%). However, to further remove antibody aggregates and/or fragments, it is then recommended to add a SEC step (see page 24-25). The purity level will then be very high (From 95% to 99%).

Featuring:

HiTrap MabSelect PrismA protein AThese columns are prepacked with MabSelect PrismA protein A chromatographyn resin that has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing efficient cleaning between purification runs.

• Enhanced binding capacity compared with other protein A resins.

• Excellent alkaline stability enables efficient cleaning and sanitization using 0.5–1.0 M NaOH.

Ion exchange chromatographyHiTrap Capto™ Q anion exchange chromatography columnHiTrap Capto Q chromatography column is packed with a strong anion exchange modern resin for high resolution, small-scale protein purification.

Item Description Ordering Code Ordering QuantityAntibody affinity chromatography HiTrap MabSelect PrismA 76237-726P 1x1mL/pkHiTrap MabSelect PrismA 76237-730P 1x5mL/pkHiTrap MabSelect SuRe™ 95055-926P 5x1mL/pkHiTrap MabSelect SuRe 95055-842P 5x5mL/pkHiTrap Protein A HP 95055-846P 5x5mL/pkHiTrap Protein A HP 95056-032P 5x1mL/pkHiTrap Protein G HP 95056-034P 5x5mL/pkHiTrap Protein G HP 95055-944P 5x1mL/pkHiTrap Heparin HP 95056-036P 5x1mL/pkHiTrap Heparin HP 95055-850P 5x5mL/pkIon exchange chromatography HiTrap Capto S ImpAct 10158-352P 5x5mL/pkHiTrap Capto SL 95056-216P 5x5mL/pkHiTrap Capto SP ImpRes 97067-778P 5x5mL/pkHiTrap Capto Q ImpRes 97067-782P 5x5mL/pkHiTrap Capto Core 700 89230-834P 5x1mL/pkMultimodal Chromatography HiTrap Capto adhere 95056-158P 5x5mL/pkHiTrap Capto Q 95055-922P 5x5mL/pkHiScreen™ Capto adhere ImpRes 89425-696P 1x4.7mL/ea

SAVE UP TO 10%

NEW

NEW

NEW

22

Size Exclusion Chromatography ColumnsSEC is typically used as a last polishing step in a protein purification protocol. We offer a wide range of gel filtration or size exclusion chromatography (SEC) media (resins). See guide to chromatography resins on page 24 and 25.

New generation SEC columns packed with Superdex Increase and Superose Increase resinsThese columns are designed for high resolution in small-scale preparative purification and analysis for sample volumes up to 500 µL.

• They deliver improved performance compared to predecessor columns:

• Reduced run time with maintained resolution

• Higher resolution for improved protein purity

• Higher lot-to-lot consistency

Item Description Ordering Code Ordering QuantitySize Exclusion Columns and ResinsHiPrep™ 16/60 Sephacryl™ S-300 HR 95056-004P 1EaHiPrep 26/60 Sephacryl S-300 HR 95055-810P 1EaSuperose 6 Increase 10/300 GL 10192-228P 1EaSuperose 6 Increase 5/150 GL 10192-230P 1EaSuperdex 75 Increase 10/300 GL 75799-300P 1EaSuperdex 75 Increase 5/150 GL 75799-302P 1EaSuperdex 200 Increase 10/300 GL 89497-272P 1EaSuperdex 200 Increase 5/150 GL 89497-274P 1EaSuperdex 30 Increase 10/300 GL 76015-026P 1EaHiLoad™ 16/60 Superdex 75 PG 71002-666P 1EaHiLoad 16/60 Superdex 200 PG 71002-670P 1Ea

SAVE UP TO 15%

Superdex 75 IncreaseRecombinant tagged proteins Fractionation range Mr ~ 3000 to 70 000

Superose™ 6 IncreaseLarger proteins and protein complexes† Fractionation range Mr ~ 5000 to 5 000 000

Superdex 30 IncreasePeptides and other small biomolecules Fractionation range Mr ~ 100 to 7000

Superdex 200 IncreasemAb and other antibodies Fractionation range Mr ~ 10 000 to 600 000

23

Desalting and buffer exchangeFeaturing:

HiPrep desalting columnsHiPrep 26/10 Desalting are convenient, easy-to-use, 53 mL columns prepacked with Sephadex G-25 Fine resin for desalting and buffer exchange.

Protein concentrationFeaturing:

Vivaspin concentratorsVivaspin spin sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Providing high recovery and flow rates.

Vivaspin volume ranges

Volume Range Product

100–500 ul Vivaspin 500

400 ul to 2 ml Vivaspin 2

2–6 ml Vivaspin 6

5–20 ml Vivaspin 20

Item Description Ordering Code Ordering QuantityDesalting and Buffer Exchange Columns HiTrap Deslating 95056-006P 5x5mLHiPrep 26/10 Desalting 95055-814P 1EaVivaspin Sample Concentrators Vivaspin 2, 5 kDa MWCO PES 95055-990P 25/pkVivaspin 2, 100 kDa MWCO PES 95055-914P 25/pkVivaspin 6, 5 kDa MWCO PES 95055-828P 25/pkVivaspin 6, 100 kDa MWCO PES 95055-836P 25/pkVivaspin 20, 3 kDa MWCO PES 95056-124P 12/pkVivaspin 20, 5 kDa MWCO PES 95056-126P 12/pkVivaspin 20, 10 kDa MWCO PES 95056-128P 12/pkVivaspin 20, 30 kDa MWCO PES 95056-130P 12/pkVivaspin 20, 50 kDa MWCO PES 95056-132P 12/pkVivaspin 20, 100 kDa MWCO PES 95056-134P 12/pk

SAVE UP TO 10%

24

Recombinant tagged

proteins

Nickel ion leakageis an issue

High bindingcapacity isthe priority

High purityis the priority

High bindingcapacity is the priority

Scalabilityis needed

High resolutionis the priority GST-tag

High resolutionis the priority

Scalability orbatch puri�cations

are needed

Ni Sepharose™ excel

TALON® Super�ow™

Ni Sepharose High Perfomance

Ni Sepharose 6 Fast Flow

Glutathione Sepharose 4B

Glutathione Sepharose High Performance

Glutathione Sepharose 4 Fast Flow

StrepTactin Sepharose High Performance

Dextrin Sepharose High Performance

Preparative puri�cation/

Scale-up with broad

choice of selectivity

Unpuri�ed sample

Sepharose Fast Flow

Q and DEAE (anion exchanger)S and CM (cation exchanger)

Sepharose High Performance Q (anion exchanger)

SP (cation exchanger)

IIon

chro

ACA�nity

chromatography

Histidine-tag

Strep-tag™ II

MBP-tag

Antibodies and antibody

fragments

Human IgG

Antibody fragments

IgG from most species

including rats

First choice forroutine puri�cation

Established process with MabSelect

SuRe or LX

First choice for optimal

performance

Scalability orbatch puri�cations

are needed

First choice for routine puri�cation

High binding capacityis the priority or need for batch puri�cation

Routinepuri�cation

Need thoroughcleaning of theresin between

puri�cation runs

Continuous processes

Capto™ L

MabSelect™ PrismAHigher binding capacity than predecessors MabSelect SuRe and LX

MabSelect SuRe™ or MabSelect SuRe LX

Protein G Sepharose High Performance

Protein G Sepharose 4 Fast Flow

MabSelect SuRe pcc

Protein A Sepharose High Performance

rProtein A Sepharose Fast Flow

Your guide to chromato Pure proteins today . Powerful results tomorrow .

GE, the GE Monogram, Capto, GraviTrap, HiLoad, HiPrep, HiScreen, HiTrap, MabSelect, MabSelect SuRe,MidiTrap, MiniBeads, MiniTrap, MonoBeads, MultiTrap, RESOURCE, Sephacryl, Sephadex, Sepharose, SOURCE, SpinTrap, Superdex, Superose, and Tricorn are trademarks of General Electric Company. Strep-tag and Strep-tactin are trademarks of IBA GmbH. TALON is a trademark of Clontech Laboratories, Inc. Superflow is a trademark of Sterogene Bioseparations Inc. All other third party trademarks are the property of their respective owners. © 2015–2018 General Electric Company.GE Healthcare Bio-Sciences AB, Björkgatan 30, 751 84 Uppsala, Sweden.

KA4444240518PO 29165132 AD

For other chromatography techniques, please visit www .vwr .com

25

Analysis or puri�cation

(µg/run)

Highest resolution (mg/run)

High resolution and high

�ow rates(≥ mg/run)

Partiallypuri�ed sample

Almost pure sample

SOURCE™ 15Q (anion exchanger)S (cation exchanger)

Preparative puri�cation

and high resolution

MiniBeads™ columnsQ (anion exchanger)S (cation exchanger)

MonoBeads™ columnsQ (anion exchanger)S (cation exchanger)

SECSize exclusion

chromatography

IEXn exchange matography

High resolution protein

separation

Superdex 30 Increase columns Mr ~100–7 000

Preparativepuri�cation

(samplevolume

< 13 mL)

Analysis and/or small-scale puri�cation

(sample volume < 500 µL)

PolysaccharidesMacromolecules

Viruses

Antibodies

Recombinanttagged proteins

Peptides and other small biomolecules

Larger proteins andbiomolecules

Protein complexes

Peptides and other small biomolecules

Antibodies

Recombinanttagged proteins

Proteins

Peptides

Sephadex™ G-25

Sephadex G-10

HiPrep™ Sephacryl™ S-500 HR columns Mr ~40 000–20 000 000 (dextrans)

HiPrep Sephacryl S-400 HR columns Mr ~20 000–8 000 000

HiPrep Sephacryl S-300 HR columns Mr ~10 000–1 500 000

HiLoad™ Superdex™ 200 pg columns Mr ~10 000–600 000

HiPrep Sephacryl S-200 HR columns Mr ~5 000–250 000

HiPrep Sephacryl S-100 HR columns Mr ~1 000–100 000

HiLoad Superdex 75 pg columns Mr ~3 000–70 000

HiLoad Superdex 30 pg columns Mr < ~10 000

Superose™ 6 Increase columns Mr ~5 000–5 000 000

Superdex 200 Increase columns Mr ~10 000–600 000

Superdex 75 Increase columns Mr ~3 000–70 000

Desalting/bu�erexchange

graphy resins

GE format guide – For ordering information, visit www .vwr .com

Type of purification Manual purification Manual or system purification System purification

Format Spin columns 96-well plates

P G S

Gravity flow columns

H P

Bottles of chromatography media (resins) Small-column cartridges Other columns

Format name SpinTrap™ MultiTrap™ GraviTrap™, MiniTrap™, MidiTrap™, PD10 Lab pack HiTrap™

HiScreen™, HiPrep, HiLoad, RESOURCE™, Tricorn™, and Precision

Use

Screening and quick

desalting of small sample quantities using a benchtop centrifuge

High-throughput screening and small scale purification using centrifuge or vacuum equipment

Simple one-step purification of proteins or sample desalting without the need for equipment

Batch purification and self-packing

Easy to use with a syringe, peristaltic pump, or a chromatography system

Larger scale or high performance applications

ANALYSIS

26

Western Blotting DetectionECL Western Blotting Detection Reagents: ECL based on horseradish peroxidase (HRP)-conjugated secondary antibodies has become the most commonly used detection method for Western blotting. It is a sensitive detection method, where the light emission is proportional to protein quantity. Minute quantities of proteins can be detected and quantitated.

• Longer shelf life: 18 month shelf life on ECL Select and Prime Products

• Stability: ECL products are stable and stored at room temp

Item Description Ordering Code Ordering QuantityECL Detection Kits ECL Select Western Blotting Detection Reagent 89233-310P For 1000 cm² membrane/EaECL Prime Western Blotting System 89168-782P For 1000 cm² membrane/EaECL Western Blotting Reagents 95038-564P For 4000 cm² membrane/EaLab Essentials VACU-GUARD 60mm 0.45µm PTFE 28137-737P 10/pkLitmus Paper Blue 7mmx5m 14217-684P 1 roll/pk105 10x15cm Lens tissue paper 97002-542P 25/pkBenchkote sheets 46x47cm 52855-103P 50/pk

SAVE UP TO 30%

Type of analysis? Detection method Protein expression level Protein expression level

High

HighLarge experimental setup Amersham ECL Prime

Amersham ECL

Amersham ECL Start

Amersham ECL Start

Scarce antibodies Amersham ECL Select

Amersham ECL Select™Low/very low

Low/very lowCCD imager

Verify presence of protein

Amersham ECL Prime

Amersham ECL Select

X-ray film

Amersham ECL Prime

Clarity Western ECL Substrate, Bio-Rad

Amersham ECL Prime exhibits higher sensitivity and signal intensity compared with Clarity Western ECL Substrate in Western blotting detection of ERK 1/2.

Samples: Two-fold dilution series of HeLa cell lysate starting at 2.5 µgPrimary antibody dilution: 1:3000Secondary antibody dilution: 1:30 000Detection: Amersham Imager 600, 3 min exposure

Amersham ECL Select

SuperSignal West Femto, Thermo Scientific

SuperSignal West Dura, Thermo Scientific

Luminata Forte, Millipore

A side-by-side comparison of Amersham ECL Select with other high-sensitive chemiluminescence detection reagents.

Samples: Two-fold dilution series of HeLa cell lysate starting at 2.5 µg

Primary antibody dilution: 1:10 000

Secondary antibody dilution: 1:100 000

Detection: Amersham Imager 600, 3 min exposure Amersham ECL Select shows brighter bands, lower background, and higher limit of detection compared with competitor reagentsin Western blotting detection of ERK 1/2.

27

Western Blotting Membranes GE Healthcare Life Sciences offers a broad selection of nitrocellulose (NC) and polyvinylidine difluoride (PVDF) Western blotting membranes, with pore sizes to suit your application requirements

• Optimized for chemiluminescent and fluorescent detection

• Excellent protein binding capacity over a wide size range

• New larger pack sizes reduce your price per blot by up to 30%

Amersham Protran™ – NC membranes with high affinity for proteins, great blocking ability, and compatibility with a range of detection methods

Amersham Protran Supported – NC membranes supported by an inert polyester nonwoven material, giving the membranes exceptional handling characteristics that allows for stripping and reprobing of the membrane

Item Description Ordering Code Ordering QuantityMembranes Protran Premium 0.2µm 300mmx4m 10119-996P 1 roll/pkProtran Premium 0.2µm 200mmx4m 10120-152P 1 roll/pkProtran Premium 0.2µm 100x100mm 10120-100P 25/pkProtran 0.45µm 300mmx4m 10063-173P 1 roll/pkProtran 0.45µm 200mmx4m 10120-148P 1 roll/pkPVDF 0.2µm 260mmx4m 10120-022P 1 roll/pkPVDF 0.45µm 300mmx4m 10061-492P 1 roll/pkProtran 0.45µm/3mm 80X90mm 10120-130P 10/pkFilm Hyperfilm™ ECL, 18x24cm 95017-655P 50 sheets/pkHyperfilm ECL, 8x10in 95017-659P 50 sheets/pkHyperfilm MP 8x10in 95017-673P 50 sheets/pk

SAVE UP TO 30%

Hypher Film MP:An excellent multipurpose film for radiometric or chemiluminescent applications.

28

SAVE UP TO 30%

Western Blotting EssentialsAmersham ECL Mouse IgG, HRP-linked whole Antibody (from sheep) HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents

• Highly species-specific

• Recommended dilutions to minimize background

Protein A Mag SepharoseProtein A Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. Based on Sepharose with native protein A as ligand.

• Visible and dense Sepharose-based magnetic beads with protein A ligand, easy to spot and collect bound target protein

• Non-adherent beads eliminate smearing effects and aggregate formation; can be used without detergents

• Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)

Item Description Ordering Code Ordering QuantityAntibodiesMouse IgG HRP-linked whole AB 95017-332P 1mL/EaHuman IgG HRP-linked whole AB 95017-326P 1mL/EaRabbit IgG HRP-linked whole AB 95017-556P 1mL/EaRat IgG HRP linked, whole AB 95017-512P 1mL/EaStreptavidin-HRP Conjugate 95017-426P 1mL/EaStreptavidin Biotinylated HRP complex 95017-422P 1mL/EaLabelling CY2-Streptavidin 95017-396P 1mg/EaCY3-Streptavidin 95017-402P 1mg/EaCY5-Streptavidin 1mg 95017-408P 1mg/EaMarkers Full Range Mol. Wt. Markers Rainbow 95040-114P 250µL/EaECL DualVue™ Western Markers 95038-574P 25 loadings/EaImmunoseparation Magnetic BeadsProtein A Mag Sepharose 89129-104P 4x500µL/EaProtein G Mag Sepharose 89129-102P 4x500µL/EaNHS Mag Sepharose 89129-100P 4x500µL/EaSera-Mag SpeedBead Protein A/G particles, 1µm, 1% solids, 1mL, 0.05% Na azide.

10204-752P 1mL/Ea

MagRack 6 89129-096P 1Ea

29

Lab EssentialsVACU-GUARD VACU-GUARD disc filters available in 50 and 60 mm formats

• Excellent for protecting vacuum pump systems from solvent vapor or gaseous contaminants and aqueous aerosols

• Designed for in-line use with stepped barb connections for 10 to 12 mm i.d. hose

• Available with choice of chemical trap: activated carbon, molecular sieve, or desiccant

Benchkote Surface ProtectorGE’s Whatman Benchkote surface protector is an absorbent lab paper that protects surfaces against hazardous spills

• Strong: Benchkote surface protector is strong and tear resistant when wet or dry

• Ease-of-use: Smooth white surface can be written on with ink or pencil and lies flat

• Clean: Suitable for saturation with disinfectant to protect benches where pathogens and other bacteria are present

• Disposable: Benchkote paper can be incinerated after use

Item Description Ordering Code Ordering QuantityBenchkote sheets 46x47cm 52855-103P 50/pkBenchkote sheets 46x57cm 52855-001P 50/pkVACU-GUARD 60mm 0.45µm PTFE 28137-737P 10/pkLitmus paper blue 7mmx5m 14217-684P 1 roll/pk105 10x15cm Lens tissue paper 97002-542P 25/pk

30

Our Lab was functioning so until we found a

in our . This wreaked havoc, as our experiments weren’t

leading to a catastrophe! We tried

to blame but it was of no use. had a

idea. We called GE and VWR to see what they could do to help us. Thanks to BioXtra,™

we were able to a of money for our Lab.

This made all of us look like to our Boss, the

person we have ever known! We all got as a gift from our Boss for saving the day. Our

experiments ended up running and we all

ever after!

Adverb

Noun

Verb Ending in ‘ing’

Name of Colleague in Lab

Adjective

Verb

Plural Noun

Adjective

Plural Noun

Adjective

Adjective

Past Tense Verb

Fictional Character

Adjective

Noun

MAD LABSLab Tales

31

Check your answers or for a little help, log onto vwr.com/gehealthcare and go to link for BioXtra

ACROSS

4. Improved purification

6. Processes 3-7 times more

7. GE benchtop surface

11. Falling off at maturity

12. Fall season

13. Protein concentrator spin

15. Colonists and wampanoag 1621

18. Affordable small volume GE

19. Easy purification automation

21. Educational system

25. Amersham western blotting

26. Celtic festival samhain

27. Biological sample collection

28. 50 years of cell culture

DOWN

1. Chemiluminescent detection

2. Vacuum pump protector

3. Yields high quality plant

5. Roots from 1946 in medicine, research, and industry

8. Convenient coupling of

9. 250 years of filtration

10. Syringe, syringe filter, vial, cap, and septum in one

14. Greek word "large melon"

16. DNA amplification kit

17. Guarders of the fields

20. Serum consistency

22. Lammas

23. King of fruits

24. NFL game

1 2 3

4

5

6 7

8 9

10

11

12

13

14

15 16

17

18

19 20 21 22

23

24 25

26

27

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CROSS CATEGORY

Distributor GE Healthcare

GE, the GE Monogram, AKTA, Amersham, Anotop, Benchkote, Cosmic Calf, ECL, ECL Advance, ECL DualVue, ECL Plex, ECL Prime, ECL Select, ExoProStar, GSTrap, GenomiPhi, GFX, FetalClone, FTA, HiLoad, HiPrep, HiScale, HiTrap, Hybond, HyClone, Hyperfilm, HyQ, illustra, MabSelectSure, Microspin, MiniTrap, Mini-UniPrep, Protran, Rainbow, Ready-To-Go, RTG, Sephadex, Sepharose, SpinTrap, Superdex, Superose, TempliPhi, UNICORN, Whatman and Whatman GD/X are trademarks of General Electric Company. TALON is a registered trademark of Clontech Inc. VivaSpin is trademark of Sartorius Stedim Biotech GmbH. All other third-party trademarks are the property of their respective owners.

*The Polymerase Chain Reaction (PCR) is covered by patents owned by Roche Molecular Systems and F Hoffmann-La Roche Ltd. A license to use the PCR process for certain research and development activities accompanies the purchase of certain reagents from licensed suppliers such as GE Healthcare and affiliates when used in conjunction with an authorized thermal cycler.

Phi 29 DNA polymerase and its use for DNA synthesis is covered by US patent numbers 5,854,033, and 5,576,204.

Ready to Go RT-PCR Beads: Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, and 6,127,155. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

© 2018 General Electric Company.

All offers in this issue are valid until November 30th , 2018

All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request.

Contact your local GE Healthcare representative for the most current information.

Every effort will be made to give reasonable notice of price changes but we reserve the right to change prices without further notice. All prices exclude value added or sales tax and may be subject to change without notice. These offers cannot be used in conjunction with any previously arranged purchase agreement.

KA3915160718BR

0818 40M Lit. No. 070043 Promo Code 4353

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Distributor GE Healthcare BioXtra 2018