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DIAGNOSTIC SERVICE PORTFOLIO
CONSULTING
AniCon Labor GmbH | Muehlenstr. 13 | 49685 Hoeltinghausen | Germany Phone +49 44 73 94 38 30 | [email protected] | www.anicon.eu
POULTRY
Dr. Swaantje Rönchen
Phone: +49 44 73 / 94 38 - 52
Dr. Diana Petzoldt
Phone: +49 4473 / 94 38 - 786
All prices are exclusive value-added tax. – Our general terms and conditions apply, available on www.anicon.eu. Hoeltinghausen, 10.03.2020
Diagnostic Service Portfolio Poultry Page 2 of 10
G.1.01, 2020.03.10 | Rev006
Pathology
Selective sampling for bacteriology
Extended sampling efforts for PCR and / or cultural virology
Pathological-anatomical reporting
Parasitologic investigation (mucosal scraping; microscopic examination)
Parasitologic investigation (flotation/sedimentation; microscopic examination)
Patho-histological examination (per tissue sample)
Material for sampling
AniCard with 10 spots (FTA®-card technology) (per card)
Reaction tube 1,5 ml (per 500 tubes)
Tube 13 ml and plug (per 500 tubes)
Salmonella-sampling swab (with neutralizer solution; per 50 swabs)
Boot/Sock swab (per 100 swabs)
Sampling beaker sterile (per 5 beaker)
Swab for anaerobic bacteria (per 50 swabs)
Fine swab (aluminium) with transport medium (bacteriology) (per 100 swabs)
Fine swab (aluminium) without transport medium (bacteriology/PCR) (per 100 swabs)
Swab (wooden) without transport medium (bacteriology) (per 100 swabs)
Swab with preservative agent (Mycoplasma or virus culture) (per 50 swabs)
Swab with preservative agent (Brachyspira) (per 50 swabs)
Paperstrips (ELISA) (per 100 strips)
All prices are exclusive value-added tax. – Our general terms and conditions apply, available on www.anicon.eu. Hoeltinghausen, 10.03.2020
Diagnostic Service Portfolio Poultry Page 3 of 10
G.1.01, 2020.03.10 | Rev006
ELISA
Fowl Adenovirus (FAdV)
Avian Encephalomyelitis Virus (AEV)
Bordetella avium (turkey)
aMPV / ART / TRT / SHS (subtypes A & B)
Chicken Anemia Virus (CAV)
Chicken Astrovirus (CAstV; cave: group B only)
Hemorrhagic Enteritis Virus of Turkeys (HEV)
Gumboro (IBDV)#
Gumboro (IBD plus; in case of vaccination with Vaxxitek® IBD)
Infectious Bronchitis Virus (IBV)
Infectious Laryngotracheitis Virus (ILTV)
Influenza Virus Type A (multispecies / blocking)
Influenza Virus - H5 specific (multispecies / blocking)
Influenza Virus - H7 specific (multispecies / blocking)
Newcastle Disease Virus (aPMV-1)
Mycoplasma gallisepticum (Mg)
Mycoplasma synoviae (Ms)
Mycoplasma meleagridis (Mm)
Ornithobacterium rhinotracheale (ORT)
Pasteurella multocida (chicken, turkey)
Riemerella anatipestifer (turkey & duck only, please indicate species)
Reovirus (ARV)
Salmonella Group B & D (incl. SE & ST; chicken)
# vaccination date prediction is provided upon request (at no extra charge)
All prices are exclusive value-added tax. – Our general terms and conditions apply, available on www.anicon.eu. Hoeltinghausen, 10.03.2020
Diagnostic Service Portfolio Poultry Page 4 of 10
G.1.01, 2020.03.10 | Rev006
HI – Hemagglutination Inhibition Testing
Egg Drop Syndrome-76 Virus (EDS-76 / DuAdV)
Influenza H-Type (H1, H5, H6, H7, H9, further upon request)
Newcastle Disease Virus (aPMV-1)
aPMV-3
SSA – Serum Slide Agglutination
Mycoplasma gallisepticum (Mg)
Mycoplasma synoviae (Ms)
Mycoplasma meleagridis (Mm)
Salmonella Gallinarum Pullorum (SGP)
All prices are exclusive value-added tax. – Our general terms and conditions apply, available on www.anicon.eu. Hoeltinghausen, 10.03.2020
Diagnostic Service Portfolio Poultry Page 5 of 10
G.1.01, 2020.03.10 | Rev006
Microbiology
Bacteriological examination (per swab / animal)
Bacteriological examination package$ 1-5 chicks / poults
Bacteriological examination package$ 6-14 chicks / poults
Biochemical differentiation - API
MALDI-TOF MS (species determination by mass spectrometry)
Resistance testing (ADT)
Resistance testing (MIC)
Archival storage (bacterial & viral strains)
Cultural enrichment – Brachyspira spp.
Cultural enrichment - Clostridium spp.
Cultural enrichment - Mycoplasma spp.
Selective cultivation of fungi (without species differentiation)
Cultivation Salmonella spp. (acc. ISO 6579)
Cultivation Salmonella spp. 5-fold passaging (in-house method)
Salmonella spp. - serovar-typing according to Kauffmann-White scheme
Salmonella Enteritidis Vaccine/Field strain-Differentiation (Erythromycin/Rifampicin-Sensitivity testing (LAH) and IDT Salmonella Diagnostics) Salmonella Typhimurium Vaccine/Field strain-Differentiation (Erythromycin/Rifampicin-Sensitivity testing (LAH) and IDT Salmonella Diagnostics)
E. coli serotyping (slide agglutination)
ORT serotyping (agarose gel precipitation)
Pasteurella multocida serotyping (acc. to Heddleston)
Riemerella serotyping (slide agglutination) $ includes cultural examination for Salmonella spp. and basic bacteriological examination (price excl. further examinations, such as resistance testing etc.)
All prices are exclusive value-added tax. – Our general terms and conditions apply, available on www.anicon.eu. Hoeltinghausen, 10.03.2020
Diagnostic Service Portfolio Poultry Page 6 of 10
G.1.01, 2020.03.10 | Rev006
Viral PCR1 & Sequencing
Avian Encephalomyelitis Virus (AEV)
Avian Influenza Type A (“Matrix-PCR”, IVA)
Influenza H3
Influenza H5 & N1 combination
Influenza H6
Influenza H7
Influenza H9
Influenza-N-type determination N1 to N9 (price per each additional N-antigen)
Influenza-H-type determination H1 to H16 (price per each additional H-antigen)
Influenza H5/H7 sequencing for HPAI/LPAI differentiation
Avian Hepevirus (aHEV/ Avian Hepatitis E virus/ BLSD virus)
Avian Metapneumovirus A & B (serotype-specific, aMPV-A & -B / ART / TRT / SHS)
Avian Metapneumovirus C & D (serotype-specific, aMPV -C & -D)
Avian Metapneumovirus A to D (serotype-specific, combination)
Avian Nephritis Virus 1 & 2 (ANV-1 & -2)
Avian Nephritis Virus 3 (ANV-3)
Avian Ortho-Reovirus (ARV)2
Avian Ortho-Reovirus sequencing & phylogenetic analysis (C- and/or NS-gene)
Avian Ortho-Reovirus S1133 DIVA3-PCR
Duck / Goose Reovirus (DRV / GRV)
Avian Polyomavirus (GHPV / Hemorrhagic Nephritis and Enteritis Virus of Geese / HNEG)
Avian Rotavirus A & D (serotype-specific, combination)
Pheasant Rotavirus
Pigeon Rotavirus
Chicken Anemia Virus (CAV)
Chicken Astrovirus (CAstV, group A & B)
Chicken Astrovirus sequencing for differentiation of group A & B
Chicken Proventricular Necrosis Virus (Transmissible Viral Proventriculitis / CPNV / TVP)
Duck / Goose Circovirus (DuCV / GoCV; species-specific)
Duck Egg Drop Flavivirus (BYDV / Baiyangdian)
Duck Enteritis Virus (Duck plague, DEV)
Duck Hepatitis A Virus Type 1 (DHAV-1 / DHV-1)
Muscovy Duck Parvovirus & Goose Parvovirus (MDPV, GPV) Screening-PCR
MDPV/GPV Typing-PCR for differentiation of Goose Parvovirus/Muscovy Duck Parvovirus
Duck & Goose Parvovirus DIVA³-PCR (Hoekstra)
1,2,3,4: For explanation and comments see page 10
All prices are exclusive value-added tax. – Our general terms and conditions apply, available on www.anicon.eu. Hoeltinghausen, 10.03.2020
Diagnostic Service Portfolio Poultry Page 7 of 10
G.1.01, 2020.03.10 | Rev006
Viral PCR1 & Sequencing (continuing)
Egg Drop Syndrome-76-Virus (EDS-76 / DuAdV)
Fowl Adenovirus (FAdV)2
Fowl Adenovirus sequencing for species and serotype determination
Fowlpox Virus (FPV)
Infectious Bronchitis Virus / Avian Coronavirus screening (incl. IBV & TuCV)
IBV-variant-specific determination (package of all available variants)4
IBV sequencing & phylogenetic analysis (S1)
Infectious Laryngotracheitis Virus (ILTV)
IBDV screening incl. patho-typing (Gumboro, differentiation between vvIBDV and non-vIBDV)
IBDV sequencing & phylogenetic analysis (VP1 & 2)
IBDV conceptual translation (12 amino acids)
IBDV conceptual translation (28 amino acids)
Innovax®-ND-IBD DIVA3-PCR
Herpes Virus of Turkeys (GaHV-3, HVT)
Marek's Disease Virus (MDV, GaHV-1)
Marek & Rispens DIVA3-PCR (separate detection of field and Rispens strain)
Marek's Disease Virus sequencing
Newcastle Disease Virus (Avian Paramyxovirus-1, aPMV-1)
Newcastle Disease Virus / aPMV-1 patho-typing-PCR (lentogenic vs. meso- & velogenic)
Newcastle Disease Virus / aPMV-1 sequencing & patho-typing (assortment to lento-, meso- or velogenic)
Reticuloendotheliosis Virus (REV)
Turkey Astrovirus 1 / 2 (serotype-specific, TAstV-1/-2)
Turkey Coronavirus (TuCV)
Turkey Hemorrhagic Enteritis (TuHEV)
Turkey Viral Hepatitis (TVH)
TVH sequencing & phylogenetic analysis
Vaxxitek® HVT+IBD DIVA3-PCR
Vectormune® ND DIVA3-PCR
1,2,3,4: For explanation and comments see page 10
All prices are exclusive value-added tax. – Our general terms and conditions apply, available on www.anicon.eu. Hoeltinghausen, 10.03.2020
Diagnostic Service Portfolio Poultry Page 8 of 10
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PCR1 and Sequencing - Diverse
Mycoplasma
Mollicutes spp.
Mycoplasma gallisepticum (Mg)
Mg DIVA3-PCR vaccine strain 6/85-like and F-like-strain
Mg DIVA3-PCR vaccine strain ts-11
Mycoplasma synoviae (Ms)
Ms-H DIVA3-PCR (incl. Ms field strain detection)
Ms-1 DIVA3-PCR
Mg & Ms (species-specific, combination)
Mycoplasma meleagridis (Mm)
Mycoplasma iowae (Mi)
Chlamydia
Chlamydia spp. screening, C. abortus & C. psittaci (species-specific, combination)
Chlamydia spp. screening
Chlamydia abortus
Chlamydia avium (doves, psittacidae)
Chlamydia gallinacea
Chlamydia psittaci
1,2,3,4: For explanation and comments see page 10
All prices are exclusive value-added tax. – Our general terms and conditions apply, available on www.anicon.eu. Hoeltinghausen, 10.03.2020
Diagnostic Service Portfolio Poultry Page 9 of 10
G.1.01, 2020.03.10 | Rev006
PCR1 and Sequencing – Diverse (continued)
Others
Fungi species determination (e.g. Aspergillus; by sequencing)
Avibacterium paragallinarum (Infectious Coryza)
Bordetella avium
Brachyspira spp.-screening
Thermophilic Campylobacter spp. (incl. C. coli, C. jejuni, C. lari, C. upsaliensis; excl. C. hepaticus)
Campylobacter coli, C. jejuni, C. lari (species-specific, combination)
Campylobacter hepaticus
Clostridium perfringens major & minor toxin genes#
Erysipelothrix rhusiopathiae (Erysipelas)
Escherichia coli
Escherichia coli 8 x APEC (virulence factors; incl. F11-antigen)
Histomonas meleagridis
Ornithobacterium rhinotracheale (ORT)
Pasteurella multocida
Pasteurella multocida subsp. septica
Pasteurella multocida capsule typing
Riemerella anatipestifer
Salmonella spp.
SGP DIVA4-PCR (serovar-specific differentiation of Salmonella Gallinarum and Pullorum and vaccine strain 9R specific) SE DIVA4-PCR (Salmonella Enteritidis serovar-specific & vaccine strains IDT and LAH/ELANCO specific) combined with ST (Salmonella Typhimurium serovar-specific)
# The following toxin genes are analysed: major toxin genes alpha, beta, epsilon & iota and minor toxin genes beta2, enterotoxin & netB.
Respiratory Pathogens - Scaffold Pricing of Packages*
PCR-package of 2 respiratory pathogens (bact. / viral)*
PCR-package of 3 respiratory pathogens (bact. / viral)*
PCR-package of 4 respiratory pathogens (bact. / viral)*
PCR-package of 5 respiratory pathogens (bact. / viral)*
PCR-package of 6 respiratory pathogens (bact. / viral)*
PCR-package of 7 respiratory pathogens (bact. / viral)*
PCR-package of 8 respiratory pathogens (bact. / viral)*
PCR-package of 9 respiratory pathogens (bact. / viral)*
* does not apply for DIVA4-PCRs, typing-PCRs or other PCR-packages and sequencing
1,2,3,4: For explanation and comments see page 10
All prices are exclusive value-added tax. – Our general terms and conditions apply, available on www.anicon.eu. Hoeltinghausen, 10.03.2020
Diagnostic Service Portfolio Poultry Page 10 of 10
G.1.01, 2020.03.10 | Rev006
Cultural Virus Isolation
Virus cultivation in cell culture / in embryonated egg (excl. PCR for confirmation)
Archival storage (bacterial & viral strains)
Combination FAdV & ARV: culture & PCR
Explanation and comments:
1 Samples of up to five individuals can be pooled. It is crucial to indicate if sending several swabs whether these are already
pooled or need to be pooled. Please find respective submission forms on www.anicon.eu → downloads. 2 Direct diagnostics of Fowl Adenovirus (FAdV) and/ or Avian Ortho-Reovirus (ARV) from raw samples by PCR methods may
lead to false negative results; we run at least three passages of virus cultivation before confirmation by PCR methods in
harvested cultural material, if not explicitly ordered for PCR only. Please see also article „combination FAdV & ARV: culture
and PCR“ in chapter virology.
3 DIVA - Differentiating Infected from Vaccinated Animals - method for differentiation of field strains from named vaccine
strains.
4 793B (CR88, 1/96, 4/91), QX (D388), D1466, Massachusetts, D274, Italy 02, Arkansas, Variant 2 (Israel 02/IS1494), Q1,
IB80, D181