Döhler Microbial Detection SolutionsProduct Portfolio

Are you looking for reliable tools and methods to ensure the quality of your product along the entire supply chain, all the way to the consumer?

Having been a supplier to and partner of the food and bever-age industry for many years, we know the various influencing factors that can impair product quality. Premium quality beverages need consistent safeguarding measures, from the raw material to filling. That is why we support our customers with a series of Quality & Food Safety Solutions in addition

to our Natural Ingredients and Ingredient Systems. Alongside the DMD® nutrient media and microbiological analyses, we provide our customers with support in technological ques-tions and scientific testing programmes to examine product stability.

Our goal is to offer you practical solutions that enable you to achieve fast and reliable quality assurance easily and cost effectively.

Do you want fast, safe and reliable detection of even minimal microbial contamination?

Cultural detection remains the preferred method for daily routine monitoring in quality assurance. It is cost effective and delivers reliable results. Culture media are also an essen-tial prerequisite for rapid molecular detection methods or microorganism identification, e.g. as liquid pre-enrichment.

Under our Döhler Microsafety Design® (DMD®) brand, we provide microbial detection solutions for almost every segment of the beverage and brewing industry. These prod-ucts have become established in the international bever-age industry over the last 30 years or more, and are both recommended by ministries and institutes and included in standard reference works by standardisation authorities, among others. This international acceptance is why our customers gain trust in the DMD® product quality.

In close cooperation with prestigious professors from inter-nationally renowned universities, institutes and laborato-ries, we develop innovative products that are tailored to market demands and fulfil your requirements.

For further information please contact our sales represent-ative or send us an email: dmd@doehler.com

DMD® products offer the following advantages:

• Ready-to-use• Easy application and evaluation• Fast, safe and reliable detection • Robust against disruptive influences• Cost effective• Quality tested for functionality• Global availability

Quality & Food Safety Solutions – Universal safety from one source!

DMD® – Döhler Microsafety Design®Dedicated microbial detection solutions for the beverage industry Simple – Fast – Safe

Do you not operate your own laboratory or do you want to have your own analysis results confirmed periodically by our laboratory?

You will save on investments and resources in the estab-lishment and maintenance of your own laboratories, space and staff. Take advantage of our comprehensive experience and benchmarks based on the large number of beverage samples which we receive from the interna-tional beverage industry for analysis.

Testing procedures, such as specific and non-specific cul-tivation, are accredited in accordance with DIN EN ISO/IEC 17025:2005 by Deutsche Akkreditierungsstelle Chemie GmbH, represented in the German Accreditation Council (DAkkS) for the subject areas of chemistry and biology.

Do you need advice on technical products or a comprehen-sive evaluation of the hygiene status of your production and filling facilities?

Take advantage of the many years of experience of our tech-nical staff and save yourself time and money.

Do you need reliable declarations, indications and above all certainty on whether the product you have developed or already filled will remain stable from a microbiological and sensory point of view throughout its entire shelf life?

Döhler Product Science has developed state-of-the-art sci-entific testing programmes to put your mind at ease. These involve modelling the conditions in retail – packaging, light, oxygen and heat – as realistically as possible.

Product Science has already conducted many thousands of stability tests and works together with a large network of partners from science and industry. Many years of experi-ence and standardised testing programmes that are subject to constant further development ensure that you receive reliable results and certainty in product development and market launch.

Please contact our sales representative about our Quality & Food Safety Solutions or send us an email: mailbox@doehler.com

Analytical Services

Analytical Services offers you the following analyses:

• Chemical-physical (e.g. Brix, pH, carbon dioxide, total acid) • Microbiological (e.g. beverage-spoiling microorganisms)• Further analysis on request

Technical Services

Our Technical Services can support you in:

• Microbiological analyses of weak spots• Sample and initial filling • Recipe modifications • Consulting for project planning

Product Science Test Programme

Product Science offers the following:

• RSLT – real shelf life test (under real-time conditions)• HALT – high accelerated life test (under accelerated extreme

conditions)• Testing programmes for packaging made from glass, cardboard, PET,

cans etc. • Subsequent objective, sensory analysis from experts, including

recommendations for action • Consulting for product and packaging development with regard to

optimum stability

Target microorganismsInvestigation material

General Drinking, table & mineral water Rinsing water Beer & beer mix beverages Wine & wine mix beverages Soft drinks (< 30% juice) Juice & nectars Dairy & milk mix beverages Production & filling facilities

Total viable micoorganism count (TVC)/isolation and cultivation of micoorganism

PCA PCA PCA PCA PCA PCA PCA PCA

CASO Agar (TSA) CASO Agar (TSA) CASO Agar (TSA) CASO Agar (TSA) CASO Agar (TSA) CASO Agar (TSA) CASO Agar (TSA) CASO Agar (TSA)

CASO Broth (TSB) CASO Broth (TSB) CASO Broth (TSB) CASO Broth (TSB) CASO Broth (TSB) CASO Broth (TSB) CASO Broth (TSB) CASO Broth (TSB)

DEV Nutrient Agar DEV Nutrient Agar

E.coli/Coliforms

LMC* LMC*

VRBL VRBL

CCA CCA

Beer and wine spoiling bacteria

NBB®-A NBB®-A NBB®-A

NBB®-B NBB®-B NBB®-B

NBB®-C NBB®-C NBB®-C

NBB®-PCR NBB®-PCR NBB®-PCR

NBB®-P NBB®-P NBB®-P

Brewers QCheck® Kit Brewers QCheck® Kit

PCR

dips

tick

DMD® Screen Hop Res + DMD® Detection Module

PCR

dips

tick

DMD® Screen Hop Res + DMD® Detection Module

Beer spoiling Lactobacilli and Pediococci

DMD® Screen Lac/Ped + DMD® Detection Module

DMD® Screen Lac/Ped + DMD® Detection Module

Megasphaera, Pectinatus DMD® Screen Pec/Meg + DMD® Detection Module

DMD® Screen Pec/Meg + DMD® Detection Module

Lactobacillus brevis DMD® Ident L.brevis + DMD® Detection Module

DMD® Ident L.brevis + DMD® Detection Module

Yeasts, moulds YGC YGC YGC YGC YGC YGC

Yeasts, moulds, bacteria

OFS OFS OFS OFS OFS OFS

SSL SSL SSL SSL SSL

TransFast® Gel TransFast® Gel TransFast® Gel TransFast® Gel

TransFast® Broth TransFast® Broth TransFast® Broth TransFast® Broth

Wort-Agar Wort-Agar Wort-Agar Wort-Agar Wort-Agar Wort-Agar

SDA SDA SDA SDA SDA SDA

PDA PDA PDA PDA PDA PDA

Alicyclobacillus

BAT-A BAT-A BAT-A

BAT-B BAT-B BAT-B

Guaiacol Detection Kit Guaiacol Detection Kit Guaiacol Detection Kit

Biofilm specific indicator microorganisms(hygiene monitoring)

NBB®-B-Am NBB®-B-Am NBB®-B-Am NBB®-B-Am

Beverage-specific microorganisms (validation of aseptic filling facilities)

AVM® & AVM® veg Powder

AVM® Broth

Product overview by microorganisms and beverage & beverage raw materials

* Water-specific faecal indicator microorganisms

Abbreviation | Product Format Description Product No. Packaging pHIncubation

T [°C] Days Condition

AVM® Broth Broth Liquid Linden Grain Medium for qualitative detection as part of validation of aseptic filling lines 2.04705.050 Tank car4.0 - 4.4

26 - 30 5 - 7 AerobicAVM® Broth, concentrated Concentrated Broth Concentrated liquid Linden Grain Medium for production of AVM® Broth 2.04755.521 Container

AVM® Powder Powder Powder for production of AVM® Broth – liquid Linden Grain medium 2.04704.872 20 kg (bucket)-

AVM® veg Powder Powder Plant based Powder for production of AVM® veg Broth - liquid Linden Grain medium 8.50872.872 20 kg (bucket)

BAT-A Agar Selective agar for isolation and microorganism count determinationApplication: Membrane filtration, pour plate and streaking method 2.04719.782 9 x 250 ml (glass bottle)

4.0 ± 0.2 43 - 47 3 AerobicBAT-B Broth Selective liquid medium for fast trace enrichment

Application: Sample enrichment (also turbid or raw material samples) 2.04717.782 9 x 250 ml (glass bottle)

Brewers QCheck® Kit Kit

Assortment of ready-to-use culture media containing LMC, NBB®-B Tubes, NBB®-B-Am Tubes and sterile swabs on wooden sticks: Qualitative detection of indicator microorganisms from water samples, fast and reliable trace detection of beer spoiling bacteria from yeast, beer and swab sampling as part of the microbiological facility monitoring Application: Qualitative pre-test and direct detection

2.11753.2442 x 50 ml (glass bottle) 2 x 20 x 10 ml tubes, incl. 30 swabs (box)

- - - Aerobic/anaerobic

CASO Agar(TSA)

Casein Soy Bean Digest Agar (Caseine-Peptone Soymeal- Peptone Agar) (Soy Bean Caseine digest Agar) / Tryptic Soy Agar

Agar Complex agar Application: Membrane filtration, pour plate and streaking method 8.43326.244 6 x 200 ml (glass bottle)

7.3 ± 0.2 29 - 31 3 Aerobic

CASO Broth(TSB)

Casein Soy Bean Digest Broth (Caseine-Peptone Soymeal-Peptone Broth) (Soy Bean Caseine digest Broth)/ Tryptic Soy Broth

Broth Complex broth Application: General broth for the cultivation of several microorganisms 8.43246.244 6 x 200 ml (glass bottle)

CCA Chromogenic Coliform Agar according to ISO 9308-1 Agar Selective agar for the quantitative detection of E. coli and Coliforms

Specific colony staining by enzymtic reaction components 8.43330.244 6 x 200 ml (glass bottle) 6.8 ± 0.2 38 1 Aerobic/anaerobic

DMD® Screen Lac/Ped

DMD® Lactobacillus Pediococcus Screen Kit Kit Molecular screening of Lactobacillus spp. and Pediococcus spp. in one kit

Visual analysis by using the DMD® Universal Detection Module 8.20576.244 48 reactions/kit (box) -

Storage: at -20°C

Time to result: < 2 h

Preenrichment necessary e. g. in NBB®-PCR

DMD® Screen Pec/Mec

DMD®Pectinatus Megasphaera Screen Kit Kit Molecular screening of Pectinatus spp. and Megasphaera spp. in one kit

Visual analysis by using the DMD® Universal Detection Module 8.20604.244 48 reactions/kit (box) -

DMD® Ident L. brevis

DMD® Lactobacillus brevis Ident Kit Kit Molecular identification von Lactobacillus brevis in one kit

Visual analysis by using the DMD® Universal Detection Module 8.20608.244 24 reactions/kit (box) -

DMD® Screen Hop Res

DMD® Hop Resistance Screen Kit Kit Molecular screening of hop resistance genes horA and horC in one kit

Visual analysis by using the DMD® Universal Detection Module 8.20607.244 48 reactions/kit (box) -

DMD® Detection Module DMD® Universal Detection Module Kit Universal lateral-flow stripes, necessary for the analysis of all DMD® Screen & Ident Kits 8.20610.244 48 reactions/kit (box) - Storage:

at +4-8°C - -

Guajacol Detection Kit Detection Kit Enzymatic test kit for detection of off-flavour-forming Alicycobacillus spp. 2.04737.991 Incl. 40 tubes (box) - 47 0-1 Aerobic

LMC Concentrated Broth

Selective broth based on the lactose peptone broth used in the German method (DIN) and described in the German Drinking Water Ordinance Application: Qualitative pre-test with integrated indicator for simple assessment

2.04713.700 9 x 50 ml (glass bottle) 7.2 ± 0.2 35 - 39 2 Aerobic

NBB®-A Agar Selective agar for isolation, cultivation and quantitative detection in filtered beer Application: Basic culture medium with integrated indicator for membrane filtration, pour plate and streaking method 2.04709.782 9 x 250 ml (glass bottle)

5.75 ± 0.15

26 - 28 5

Anaerobic

NBB®-BNBB®-B Broth Selective liquid medium for fast trace detection in yeast culture and filtered beer

Application: Sample enrichment with integrated indicator for simple assessment 2.04710.782 9 x 250 ml (glass bottle)Aerobic/anaerobic

NBB®-B Tubes Broth Selective liquid medium for fast trace detection in yeast culture and filtered beer Application: Sample enrichment with integrated indicator for simple assessment 2.04723.646 20 x 10 ml (tubes)

NBB®-B-Am NBB®-B-Am Broth Culture medium for qualitative detection of indicator microorganisms using swab sampling with sterile swabs as

part of microbiological hygiene monitoring 2.04706.782 9 x 250 ml (glass bottle)25 - 29 3 Aerobic

NBB®-B-Am Tubes Broth Culture medium for qualitative detection of indicator microorganisms using swab sampling with sterile swabs as part of microbiological hygiene monitoring 2.04706.646 20 x 10 ml (tubes)

NBB®-C Concentrated Broth

Concentrated, selective liquid medium for fast trace detection in unfiltered beer Application: Sample enrichment with option of adjusting requested selectivity 2.04711.782 9 x 250 ml (glass bottle) 6.5 ± 0.3

26 - 28 7 Aerobic/anaerobicNBB®-P Powder Powder for the production of NBB®-Agar and NBB®-Broth Selectivity adjustment by using own beer 2.04716.462 300 g (bag) -

NBB®-PCR Broth Pre-enrichment culture medium for a fast PCR and real-time PCR detection of beer spoiling bacteria 7.85420.782 9 x 250 ml (glass bottle) 5.7 ± 0.2Nutrient Agar in accordance with DEV Agar Complex agar, which meets the requirements of §35 LMBG, the DEV - German standard method - and DIN 38411

Application: Membrane filtration, pour plate and streaking method 2.04726.782 9 x 250 ml (glass bottle) 7.2 ± 0.2 35 - 39 or 20 1 - 3Aerobic

OFS Agar Selective agar, based on Orange Serum Agar for cultivation and determining the total viable count in beveragesApplication: Membrane filtration, pour plate and streaking method 2.04707.782 9 x 250 ml (glass bottle) 5.0 ± 0.2 25 - 29 3

PCA Plate Count Agar Agar Complex agar application: Membrane filtration, pour plate and streaking method 8.43221.244 6 x 200 ml (glass bottle) 7.0 ± 0.2 29 - 31 3 Aerobic/anaerobicPDA Potato Dextrose Agar Agar Selective agar for the isolation of yeasts and moulds 8.43329.244 6 x 200 ml (glass bottle) 5.6 ± 0.2

25 - 29

2 - 5 Aerobic

SDA Sabouraud 4% Dextrose Agar (Sabouraud 4% Glucose Agar) Agar Selective agar for the isolation of yeasts and moulds 8.43327.244 6 x 200 ml (glass bottle) 5.6 ± 0.2 3 - 5 Anaerobic

SSL Broth Selective broth for the fast trace detection Application: Sample enrichment, examination of swab samples 2.04712.782 9 x 250 ml (glass bottle) 5.0 ± 0.2 2

Aerobic

TransFast® Broth Broth Selective broth for fast and qualitative sample enrichmentApplication: Sample enrichment, examination of swab samples 2.04727.782 9 x 250 ml (glass bottle) 6.1 ± 0.2 1 - 3

TransFast® Gel Gel Selective gel for cultivation, fast and qualitative detection of beverage spoiling microorganismsApplication: Direct detection 2.04731.782 9 x 250 ml (glass bottle) 4.3 ± 0.2 1 - 2

TransFast® Starter Kit KitAssortment of ready-to-use culture media containing TransFast® Broth, TransFast® Gel and sterile TransFast® Gel Tubes for a fast and reliable detection Application: Sample enrichment and direct detection

2.11754.244 5 x 250 ml + 20 tubes (box) - 2 - 5

VRBL Violet Red Bile Lactose Agar Agar Selective agar for the quantitative detection of E. coli and Coliforms Application: Agar with integrated colour for simple assessment 8.43331.244 6 x 200 ml (glass bottle) 7.4 ± 0.2 30 or 37 1

YGC Yeast Extract Glucose Chloramphenicol Agar Agar Selective agar for the quantitative detection of yeasts and moulds

Surpression of bacterial growth by antibiotics 8.43328.244 6 x 200 ml (glass bottle) 6.6 ± 0.2 23 - 27 3 - 5

Wort-Agar Agar Agar for isolation and microorganism count determination of yeasts and moulds Application: Basic culture medium for membrane filtration, pour plate and streaking method 8.40360.782 9 x 250 ml (glass bottle) 4.8 ± 0.2 27 - 29 3 - 5

Laboratory accessories

Swabs, without tubes - Sterile cotton, swabs on wooden sticks, individually packaged Application: Swab sampling 2.04725.244 100 pc. (bag)

- - - -TransFast® Gel Tubes (75 ml) - Sterile transparent plastic tube, disposable Application: Sterile vessel for sampling with TransFast® Gel 2.04730.001 100 pc. (bag)

TransFast® Incubation Light Box - Incubator with background lighting, for use with the TransFast® system Application: Incubation of microbiological samples up to 45°C 4.40000.000 1 pc.

Alphabetic overview of the DMD® products

Under our Döhler Microsafety Design® (DMD®) brand, we provide microbial detection solutions for a wide range of beverages and beverage ingredients. Are you interested in more information on the DMD® Screen and Ident Kits,

NBB®-PCR Broth or our other innovative culture media for beverages? Visit our website at www.doehler-dmd.com or get in touch by email at dmd@doehler.com

Döhler Microbial Detection Solutions

Reliable

Fast

Ready-to-use

Quality controlled

Döhler (www.doehler.com) is a global producer, marketer and provider of technology-driven Natural Ingredients, Ingredient Systems and Integrated Solutions for the food and beverage industry. Döhler’s integrated approach and the broad product portfolio are the optimal basis for innovative and safe food & beverage applications. The product portfolio of Natural Ingredients ranges from natural flavours, natural colours, health & nutrition ingredients, cereal ingredients, dairy ingredients, speciality ingredients, dry ingredients, fruit & vegetable ingredients to Ingredient Systems.

Headquartered in Darmstadt, Germany, Döhler is active in over 130 countries and has 30 production sites, as well as sales offices and application centres on every continent. More than 5,000 dedicated employees provide our cus-

tomers with fully Integrated Food & Beverage Solutions from concept to realisation.

“WE BRING IDEAS TO LIFE.” briefly describes Döhler’s holistic, strategic and entrepreneurial approach to innovation. This comprises market intelligence, trend monitoring, the devel-opment of innovative products and product applications, advice on food safety and microbiology, food law as well as Sensory & Consumer Science.

About Döhler

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