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Page 1 of 83 Developing an Efficient Method for Generating Facial Reconstructions Using Photogrammetry and Open Source 3D/CAD Software. Cahill HUNT A thesis submitted in fulfillment of the requirements for the degree of Master of Forensic Science (Professional Practice) In The School of Veterinary and Life Sciences Murdoch University Supervisors: Dr. John Coumbaros Brendan Chapman Semester 2, 2017

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Developing an Efficient Method for Generating Facial Reconstructions Using Photogrammetry and Open Source

3D/CAD Software.

Cahill HUNT

A thesis submitted in fulfillment of the requirements for the degree of Master of Forensic Science (Professional Practice)

In

The School of Veterinary and Life Sciences

Murdoch University

Supervisors:

Dr. John Coumbaros

Brendan Chapman

Semester 2, 2017

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Declaration

____________________________________________________________________________

I declare that this thesis does not contain any material submitted previously for the award of

any other degree or diploma at any university or other tertiary institution. Furthermore, to

the best of my knowledge, it does not contain any material previously published or written

by another individual, except where due reference has been made in the text. Finally, I

declare that all reported experimentations performed in this research were carried out by

myself, except that any contribution by others, with whom I have worked is explicitly

acknowledged.

Signed: Cahill Thomas Hunt

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Acknowledgements

____________________________________________________________________________

I would like to thank my supervisors, Dr. John Coumbaros and Brendan Chapman for their

guidance throughout the entire project. Your insights, kindness, and feedback are sincerely

appreciated as well as the time you took to hear and aid with all my concerns during the

semester. I would also like to thank my peers, family, and friends, for all their support,

specifically Lena Tran, Veronica D’Souza, Joanna Macleod, and Nathan Juriansz.

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Table of Contents

Title Page ............................................................................................................................................. i

Declaration ......................................................................................................................................... ii

Acknowledgements ........................................................................................................................... iii

Part One

Literature Review ............................................................................................................... 6-58

Part Two

Manuscript ....................................................................................................................... 60-83

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Part One

Literature Review

Developing an Efficient Method for Generating Facial Reconstructions Using Photogrammetry and Open Source 3D/CAD Software.

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ABSTRACT

The identification of deceased individuals is important in society as it not only facilitates the

progression of criminal investigations into suspicious deaths, but also enables the resolution

of legal matters and brings closure to the families affected by the death. When a corpse is

skeletonized, heavily burned, or the soft tissue has degraded to a point that other

professionals cannot obtain information about the deceased, a forensic anthropologist or

odontologist is often tasked with identification. A variety of methods exist that enable

forensic anthropologists to achieve identification. These include: non-imaged records

comparisons; craniofacial superimposition and comparative radiography. Facial

reconstructions can also be utilized when no ante mortem information about the deceased

individual is available or when law enforcement have no suspicions on who the deceased

person is. Facial reconstructions are traditionally a manual method however with the recent

advancement of photogrammetry and three-dimensional and computer-aided design

modeling software, the process can be performed within a virtual space. The purpose of this

literature review is to identify an efficient and low-cost method of generating facial

reconstructions using photogrammetry and open-source three-dimensional and computer-

aided design software.

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Table of Contents

List of Figures ........................................................................................................................................... 9

List of Tables ............................................................................................................................................. 9

List of Abbreviations ............................................................................................................................... 10

INTRODUCTION ...................................................................................................................................... 11

DISCUSSION ............................................................................................................................................ 13

Introduction to Forensic Anthropology: ............................................................................................ 13

Building a Biological Profile: ............................................................................................................... 14

Ancestry Classification......................................................................................................................... 14

Sex Identification ................................................................................................................................. 17

Determination of Age .......................................................................................................................... 19

Calculation of Stature .......................................................................................................................... 22

Personal Identification: ...................................................................................................................... 23

Craniofacial Superimposition .............................................................................................................. 24

Nonimaged Records Comparison ........................................................................................................ 24

Comparative Radiography ................................................................................................................... 25

Facial Reconstructions: ...................................................................................................................... 27

Anthropometry: .................................................................................................................................. 31

New Technology and Facial Reconstructions: ..................................................................................... 33

Photogrammetry: ............................................................................................................................... 35

Close Range Photogrammetry ............................................................................................................ 37

Local Applications: ............................................................................................................................... 41

Broad Applications and the Future: ................................................................................................... 42

EXPERIMENTAL AIMS AND DESIGN ........................................................................................................ 45

Aims and Objectives: .......................................................................................................................... 45

Experimental Design: ......................................................................................................................... 45

Photogrammetry ................................................................................................................................. 45

3D/CAD classification and modeling ................................................................................................... 46

Experimental Design ........................................................................................................................... 46

CONCLUSION .......................................................................................................................................... 47

REFERENCES ........................................................................................................................................... 49

APPENDICIES .......................................................................................................................................... 55

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List of Figures

Figure 1. Craniometric landmarks for 3D analysis. Definitions are provided in Appendix 2. a

Norma Lateralis; b Norma Frontalis; c Norma Medialis; d Norma. From (59). ............................. 32

Figure 2. 3D computerized facial reconstruction procedure following the Combination

method. From (54). ....................................................................................................................... 34

Figure 3. Principle of photogrammetric measurement. From (62). ............................................. 36

Figure 4. 3D visualization of the match and the impact angle of the of the injury-causing tool

with the two impression fractures. From (7). ............................................................................... 39

Figure 5. The bite mark and the digitized casts of the suspects were examined with respect to

matching shapes, angles and dimensions in the 3D. From (8). ..................................................... 40

List of Tables

Table 1. Anthroposcopic Characteristics of the Skull of the Three Mains Ancestral Groups in

the United States. Adapted from (1) …………………………………………………………………………………….9.

Table 2. Classic Traits of the Male Versus Female Pelvis. From (1) After Table 37 (15).………10.

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List of Abbreviations ___________________________________________________________________________________

CAD Computer Aided Design

CRP Close Range Photogrammetry

CT Computed Tomography

DNA Deoxyribonucleic Acid

FSTT Facial Soft Tissue Thickness

GD General Duty

GPS Global Positioning System

MRI Magnetic Resonance Imaging

PMI Post Mortem Interval

SWGANTH Scientific Working Group for Forensic Anthropology

3D Three-Dimensional/Three-Dimensions

2D Two-Dimensional/Two-Dimensions

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INTRODUCTION

The identification of osseous remains is a standard requirement of forensic anthropologists.

In our society, the identification of deceased not only facilitates the progression of criminal

investigations but it allows the settlement of legal issues, and most importantly brings

closure to the families of the individual (1, 2). Identification is often achieved using

radiography, analysis of DNA and dental records, or facial reconstructions (3). In the

circumstance where a lack of information prohibits a practitioner from performing these

methods, a forensic anthropologist can use facial reconstructions as a reliable method of

identification (3). An important first step following the discovery of osseous remains is the

construction of a biological profile (4). A trained forensic anthropologist can glean a

significant amount of information in a short time from a gross visual examination of unknown

remains. This information is critical in an investigative context and can be utilized by

scientists to attain identification, specifically in the selection of facial soft tissue thickness

(FSTT) data for facial reconstructions, and, in turn, by law enforcement to include or exclude

suspects from further inquiry (1, 2).

Recently, forensic scientists have recognized photogrammetry as a valuable tool used to

document small forensically relevant objects (5-9). Photogrammetry involves generating a

three dimensional (3D) representation of an object from two dimensional (2D) images that

can then be imported into 3D computer aided design (3D/CAD) software where the object

can be manipulated to make comparisons (10, 11). Such comparisons include but are not

limited to: injury-causing weapons to wound morphology, skin and bone injuries to injury-

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causing objects, and bite mark impressions to dental structure (5-9). Becker (12) applied this

process to facial reconstructions in a case study where he generated a 3D model of a skull

using photographs. He then imported the 3D model into the opensource 3D/CAD software

Blender® applying soft tissue to generate a face.

The ability to document an object digitally provides significant benefits to law enforcement

officers as they have access to high-quality digital cameras and can easily be instructed to

perform the method of photography required for this technique. Law enforcement officers

would then be able to quickly transfer them to an individual with the expertise to process the

photos into a 3D model. This model could then undergo soft tissue remodeling in 3D/CAD

software to generate facial reconstructions that may be shown to the public to aid in the

identification process.

There are many methods a forensic anthropologist can utilize to generate a facial

reconstruction. Recently the reliability of the traditional manual methods has been criticized

for relying too heavily on artistic interpretation. This literature review will assess the current

state literature with an aim to develop a method of generating facial reconstructions using

photogrammetry and 3D/CAD software.

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DISCUSSION

Introduction to Forensic Anthropology:

Forensic anthropology is a sub-discipline of biological and physical anthropology (1, 2). It has

a broad foundation of literature and a strong academic history which defines it as a refined

discipline of science (13). Forensic anthropologists acquire multi-disciplinary expertise during

their studies which allow them to identify the sociocultural and human skeletal biology

determinants of human variation. They are regularly required to aid the medico-legal

community in cases of human death where the remains are skeletonized, heavily burned, or

the soft tissue of a corpse has degraded to a stage where other specialists, such as forensic

pathologists, cannot determine critical information about the deceased (1, 2). The time of a

forensic anthropologist is primarily spent aiding the medico-legal community; however, they

may also contribute to historical research. In our society, a forensic anthropologist can utilize

their expertise to offer calculated insights into cases of suspicious death, single and mass

grave excavations, mass disasters, and human rights work (1, 2).

Byers (1) established five objectives that a forensic anthropologist should endeavor to

achieve after the discovery of osseous remains. These include: (i) construct a biological

profile; (ii) determine the cause and manner of death; (iii) determine the post mortem

interval (PMI); (iv) recover all osseous remains; and (v) achieve an identification. In order to

achieve these objectives forensic anthropologists utilize their knowledge of the human

skeleton to identify morphological markers on bones that indicate how an individual may

have lived, and also the circumstances that surrounded their death (2). Establishing these

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objectives contribute to the resolution of medico-legal investigations, however, frequently

only some of the objectives are achieved.

Building a Biological Profile:

Snow (4) first summarized a method for processing osseous remains to ensure that the

maximum amount of information is gathered. As the discipline of forensic anthropology has

developed this protocol has been altered in sequence, and additional steps have been made

to reflect the current state of research best. A key element of Snow’s approach is to build a

biological profile of the deceased individual which includes determining the ancestry, sex,

age, and stature of the deceased, which are often critical in aiding law enforcement in

achieving identification (1, 2, 12). The identification of remains at advanced stages of

decomposition is often attained using the expertise of a forensic odontologist, who compare

the dentition of the deceased individual to dental records on file (2). If such records do not

exist there are several methods a forensic anthropologist can use to achieve an

identification. These include a comparison of anomalies found on the skeleton to medical

records of suspected victims, radiographic image comparisons, and facial reconstructions (2,

3).

Ancestry Classification

The human skull has unique features that vary depending on the ancestry of the individual.

Historically, the identification of ancestry has been a long-standing issue in forensic

anthropology as the morphological differences that are distinct to groups (i.e. Caucasian,

Negroid, and Mongoloid) are difficult to quantify (14). Even so, classifying the ancestry of

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osseous human remains is a standard first step forensic anthropologist must make in

constructing a biological profile as it influences the determination of following factors (1, 2).

In cases where the remains include an intact skull or a reconstructed skull that was damaged,

craniometric data is the most common data used to estimate ancestry (1, 2). Early

anthropologists identified features of the cranium such as, the nose, face, cranial vault, jaws,

and teeth that can be used to distinguish between the three ancestral groups (15, 16) (Table

1).

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Table 1. Anthroposcopic Characteristics of the Skull of the Three Mains Ancestral Groups in the United States. Adapted from (1).

Structure Caucasian Negroid Mongoloid

Nose

Root

Bridge

Spine

Lower border

Width

Face

Profile

Shape

Eye orbits

Lower eye border

Vault

Browridges

Muscle marks

Vault sutures

Postbregma

Jaws and teeth

Jaws

Palatal Shape

Upper incisors

High, narrow

High

Pronounced

Sharp (sill)

Narrow

Straight

Narrow

Angular

Receding

Heavy

Rugged

Simple

Straight

Small

Parabolic

Spatulate

Low, rounded

Low

Small

Guttered

Wide

Projecting

Narrow

Rectangular

Receding

Small

Smooth

Simple

Depressed

Large

Hyperbolic

Spatulate

Low, ridged

Low

Small

Flat, sharp

Medium

Intermediate

Wide

Rounded

Projecting

Small

Smooth

Complex

Straight

Large

Elliptical

Shoveled

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Various methods have been developed to improve the accuracy of ancestry estimation,

which involve measuring cranial landmarks and generating databases of averages for varying

populations. Recently, Sholts (14) developed a computerized method of identification

whereby a geometric plane is passed through several cranial landmarks (the nasion, and the

right and left zygomaxillary) for 3D represented crania. The contour was then analyzed,

allowing the successful identification of the ancestral groups the crania belong to.

Sex Identification

There are two primary biological differences between males and females, their size and

architecture, which reflects in their skeletons (1, 2). Forensic anthropologists can use their

knowledge of this sexual dimorphism to accurately identify the sex of osseous remains (15,

17, 18). Sex can be determined using various parts of a skeleton, however, in general, the

pelvis is the prominent indicator, specifically the pubic bone (15, 17-19) (Table 2). Phenice

(19) developed a method of determining the sex of deceased individuals using the pelvis with

an accuracy of 95%. Klales, Ousley (20) later revised this to clarify the morphological states of

the pelvis and generate a statistical foundation that can be utilized in courtrooms. The

Phenice (19) method of identifying sex involves the analysis of three structural features of

the subpubic region of the skeleton: the ventral arc, the medial aspect of the ischiopubic

ramus, and the subpubic concavity. When features of the pelvis are analyzed conclusions

from the combined data have a high degree of accuracy in identifying the sex of the remains

(1, 2).

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Table 2. Classic Traits of the Male Versus Female Pelvis. From (1) After Table 37 of (15).

It is possible that the discovery of osseous remains may not yield a complete skeleton for

various reasons including damage/alteration due to human or animal activity, and

environmental conditions. In cases where the pelvis is not present, the skull is considered the

adequate alternate area of the body to use for sex identification (2). The differences between

the male and female cranium can be attributed to the size difference between the sexes (21).

Buirkstra and Ubelaker (21) identified five structural features of the skull that can be used to

determine the sex of the remains. These include: the supraorbital margin, the nuchal crest,

the glabellar region, the mastoid process, and the mental eminence. Using the skull solely to

identify the sex of remains can be problematic without drawing comparisons between other

individuals of the same population of the deceased. This is because morphological

differences exist on a spectrum with distinct and identifying differences rarely occurring (22,

23).

Trait Males Females

Size

Ilium

Pelvic inlet

Pubic shape

Subpubic angle

Obturator foramen

Greater sciatic notch

Preauricular sulcus

Shape of sacrum

Large and rugged

High and vertical

Heart Shaped

Narrow and rectangular

V-shaped

Large and ovoid

Narrow

Rare

Long and narrow

Small and gracile

Low and flat

Circular or elliptical

Broad and square

U-shaped

Small and triangular

Wide

Well developed

Short and broad

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Determination of Age

The determination of the age of osseous remains can be a complicated step in the

construction of a biological profile. Forensic anthropologists must be familiar with the

concept that there is a difference between physiological age and chronological age.

Physiological age refers to the age of an individual’s skeleton when aligned with the typical

sequence of morphological changes it undergoes from the birth of the person until their

death, while chronological age refers to the amount of time that has passed from the birth of

a person until their death (1, 2).

There is a multitude of methods a forensic anthropologist can utilize to determine the age of

osseous remains, and the method used is often dependent on what bones of the deceased

individual’s skeleton are present (1, 2). The indicators a forensic anthropologist may use to

determine the age of a skeleton are a result of the morphological changes that are a

consequence of growth (including dentition) or changes that occur during the aging process

that appears degenerative, often associated with age-related pathologies (1). The Scientific

Working Group for Forensic Anthropology (SWGANTH) (24) has identified criteria that are

required for indicators of age to be acceptable. These include: (i) morphological changes

must proceed unidirectionally with age; (ii) features must have a high correlation with

chronological age; (iii) changes must occur at roughly the same age for all individuals (at least

within a distinguishable sub-population); and (iv) the characteristics are measured or

classified with known intra- and inter-observer error rates. Methods of aging osseous

remains can be separated into those that determine the age of subadults (individuals that are

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still in a stage of growth) and adults (individuals that have completed growth and the

skeleton has begun to deteriorate) (1, 2).

Due to being in a state of growth, there are several methods forensic anthropologists can use

to identify the age of subadults. These methods include the analysis of dentition, long bone

measurements, and the appearance of the epiphyseal union and ossification centers (1, 2).

SWGANTH (24) protocol suggests that if possible, the analysis of dentition should be utilized

first to determine the age of subadults because dentition has a high correlation with

chronological age. Researchers have identified characteristics of tooth formation that allow

forensic odontologists to determine the age of a subadult. The development of an

alphanumeric scoring system allowed the identification of the level of formation of the

crown, root, and apex of deciduous and permanent teeth through the understanding of

tooth mineralization and eruption sequence characteristics (25, 26). Tooth eruption refers to

the process teeth undergo upon reaching a certain size causing them to erupt from their

crypts into the mouth. Over time there have been adjustments made to this scoring system,

and in 2010 Al Qahtani et al. (27) used dry bone specimens and radiographs to develop an

atlas that linked the age of individuals to the developmental and eruption stages of teeth (21,

28, 29).

In circumstances where dentition is not present forensic anthropologists can use long bone

measurements to estimate the age of osseous remains. This method is particularly accurate

on fetal bones as the length of a fetus’s femoral shaft has a linear relationship with the age of

the fetus (17). After birth, bone growth slows resulting in this method being less accurate

(30).

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The appearance of the epiphyseal union and ossification centers is another method forensic

anthropologists can utilize to estimate the age of remains. Ossification centers are regions of

the bone that begin ossifying during the growth of an individual (1, 2). When they begin to

fuse with one another, the cartilage that joins the epiphyseal caps to the diaphysis of long

bones beings to ossify leaving a visible line. The fused bone undergoes remodeling, which

progresses with age and can be used to estimate the age of skeletons between 10 and 25

years old (21, 31). Studies have noted that the estimated age range obtained using the

epiphyseal union method is dependent on the sex of the remains (31).

The methods available to a forensic anthropologist to age adult remains differ from those

used for subadults because dentition has fully developed and skeletal growth has ceased (1,

2). In 2012 a study conducted by Garvin and Passalacqua (32) established four of the best

methods to age osseous remains of adults. The first of these methods involves the analysis of

the symphyseal surface of the pubis. Alterations occur to this surface that causes

morphological changes throughout adult life that can be used to estimate chronological age

(33). The second of these methods involves the analysis of sternal rib ends. Studies have

shown that the cartilage that joins the ribs to the sternum ossifies over time which causes

changes to the surface bone that forensic anthropologists can utilize to estimate age (34, 35).

Another of these methods involves the analysis of articular surfaces. The sacroiliac joint

contains an articular surface that changes during aging, and this was first noticed and related

to chronological age by Lovejoy et al. (36). Articular surfaces can be particularly useful as age

indicators because these surfaces usually survive destructive deaths and are applicable

regardless of sex (37). The last of these methods involves the analysis of cranial surfaces.

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Various studies have linked changes in morphology of the cranial vault to the chronological

age of individuals (33, 36). Buirkstra and Ubelaker (21) identified that the sutures of three

regions of the skull (endocranium, palate, and ectocranium) ossified in a manner that

allowed the stage of their ossification to be linked roughly to chronological age. In 2011

Shirley and Jantz (38) identified that the spheno-occipital suture closes during puberty and

has a strong relationship to the chronological age of young adults. Recently these

observations have been confirmed in a study on an Australian population conducted by

Lottering et al. (39).

Calculation of Stature

There are two standard methods a forensic anthropologist may use to calculate the

antemortem stature of a deceased individual. When the majority of the skeleton is present

and the sex and ancestry of the deceased are not known the anatomical method is used to

calculate stature (1). This approach was first suggested by Dwight (40), however since then it

has been revised by various researchers (41). The parts of the skeleton that are required to

perform this method include the cranium, all vertebrae (except the atlas), sacrum, femur,

tibia, talus, and calcaneus. The stature of the deceased is calculated after the bones are

measured, and the correction factor for soft tissue is incorporated (1, 2). This method is the

most accurate method of the two available.

The mathematical or regression method is the second technique a forensic anthropologist

can utilize when calculating the stature of a deceased individual when the skeleton is

incomplete. This method requires the measurement of long bones and the application of the

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relevant formula with reference to the sex of the skeleton and information based on the

temporal population it belongs to (1, 2, 42). Wilson et al. (42) have shown that the lower limb

long bones are more appropriate for this method compared to the upper limb long bones.

The construction of a biological profile of a deceased individual is a crucial objective that

forensic anthropologists aim to achieve after the discovery of osseous remains (1, 4). The

information from the techniques and methods briefly discussed above can be instrumental in

police investigations, possibly resulting in the identification of the remains. If the information

gathered from a biological profile does not initially lead to identification, there are different

methods professionals can utilize to achieve this. It is important to note that even though the

information gathered from a biological profile may not be directly responsible for the

identification, forensic anthropologists utilize it in the other methods they employ to attain

identification.

Personal Identification:

As discussed, the identification of unknown remains is a critical issue in medico-legal

investigations. A forensic anthropologist or forensic odontologist is often tasked with

identification when the remains are in a state where the soft tissue is degraded or absent (1,

2). The methods a forensic odontologist uses to obtain identification when dentition is

present were briefly discussed. This section of the literature review will focus on the methods

forensic anthropologists can utilize to achieve identification. Although the process of

constructing a biological profile may appear to be lengthy, a significant portion of the

information can be gleaned from the remains by a trained forensic anthropologist during a

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gross visual examination (1, 2). The methods available for identification can be performed in

parallel with the construction of a biological profile as the information required becomes

available. In a coordinated investigation, there would be strong communication channels

between the investigative team and the forensic team that enabled this.

Craniofacial Superimposition

Craniofacial superimposition is a method of identification that does not have to be

conducted by a forensic anthropologist. However, the practitioner that is conducting the

analysis needs to know human cranial anatomy and the variation that exists on the cranium

between human populations. An investigative team can utilize this method in circumstances

where there are suspected individuals the remains belong to and where antemortem data of

those people exist (1, 2, 43). A practitioner can then compare an antemortem image of a

known individual to a skull, or a representation of a skull (44). It is standard that two digitized

images are used to complete this which enables them to be superimposed and aligned using

cranial landmarks. Following this, a practitioner can analyze the lines and curvature of the

face and dental structure which reveals any discrepancies between the postmortem and

antemortem materials (1, 2). This method is primarily used to substantiate other

identification methods, however, in rare circumstances, it can be used to attain identification

(43).

Nonimaged Records Comparison

It is likely in a human lifespan that an individual will sustain an injury to the body or be

affected by a pathology. Many injuries and pathologies leave identifying traits on bones in

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the form of lesions, wounds, defects, or anomalies which can be used as a comparative tool

to identify osseous remains (2). Fortunately, in our society, it is a priority to keep detailed

medical records when a patient receives treatment or undergoes procedures for injuries or

pathologies. Often these records are stored digitally in the form of notes, or as radiographs

that a forensic anthropologist can use to identify remains (1, 2). When identifying traits are

discovered on remains a standard procedure for a forensic anthropologist is to obtain

medical records, with the help of law enforcement, on the suspected individuals, which can

be used to identify consistencies or inconsistencies between the materials. For these to be of

use SWGANTH (24) protocol states that they must meet two criteria, that (i) the trait must

have been documented in vivo, and (ii) the frequency of the condition must be known.

In some cases, it is possible that no identifying traits exist on an individual’s skeleton, or a

lack of antemortem data prevents conducting comparisons. Under these circumstances, a

forensic anthropologist may use the information from a biological profile to create a

template of the biological characteristics of an individual that an investigating team may use

to include or exclude suspects from further investigation (1, 2).

Comparative Radiography

Due to radiographs being a standard diagnostic tool in medicine, they are readily available to

use as comparative materials in forensic investigations (2). They can be used to achieve

identification when postmortem and antemortem radiographs are compared to match

individualizing features on osseous remains (45). This method can be performed with other

medical imaging materials such as CT and MRI and Pfaeffli et al. (46) demonstrated that this

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comparison is not limited to images derived from the same method. This study was

preliminary and included limitations including a small sample size, and in rare circumstances,

pathological processes masked or changed identifying features on the bone in the time

between antemortem and postmortem imaging.

It is standard procedure for forensic anthropologists to attain a medical history of individuals

the remains are suspected to belong to when individualizing features are identified on

remains. This process is often aided by law enforcement as legal authority is required to

obtain private information (1, 2). If an antemortem radiograph of an individualizing feature

exists, a forensic anthropologist should take a radiograph of the identifying feature on the

remains that is suspected to be a match, at the same magnification and orientation as the

original radiograph. This method of identification is a visual method, and SWGANTH (24)

protocol suggests that only practitioners with adequate experience should perform the

comparisons (2).

As discussed, there are various methods a forensic anthropologist can utilize to obtain

identification of unknown osseous remains. However, it is possible that identification cannot

be achieved using these methods as they all require antemortem information about the

deceased or suspected persons. It is possible that the deceased individual is unknown and

the investigative team has no suspicions of who the individual may be. Additionally, the

investigative team may have suspicions about who the remains are, however, no identifying

traits, antemortem data, or data on national databases exists that can be used for

comparisons that may achieve an identification (3). In these circumstances, a forensic

anthropologist may perform a facial reconstruction, which an investigative team may utilize

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in their investigation to aid in achieving identification of the remains (12). The following

section of this review will discuss in depth the processes involved in generating facial

reconstructions and how modern technology is enabling possible new and more efficient and

accurate methods.

Facial Reconstructions:

Facial reconstructions were first attempted in the late 19th century and were known to be a

combined effort of an anatomist and a sculptor (2, 3). As a result, early methods of facial

reconstructions were associated with subjectivity, since the soft tissue of the skull, and the

facial organs were represented with an artistic influence rather than being an objective

representation generated from statistically reliable data (13, 47, 48). Researchers in the field

have been developing ways to minimize the subjectivity of facial reconstructions as the

reliability of forensic methods must be maintained against the scrutiny of courtroom

proceedings. Ultimately, researchers aim to eliminate all subjectivity from the method (48).

Currently, facial reconstructions are developed using a multitude of techniques (49, 50).

Several well-established methods exist which include the American, Russian, and Combined

methods. The American method was developed by Wilton Krogman and is known for utilizing

FSTT data (15). Scientific literature contains FSTT data from various populations which in

combination with the American method can be used to build facial reconstructions by

applying a soft tissue analog to craniofacial landmarks that have been identified on a skull

(47). Mikhail Gerasimov developed the Russian method which requires knowledge of the

muscles of the face and neck and is characterized by modeling these muscles one by one to

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generate a facial reconstruction (1, 2). The last method was developed by Richard Neave and

is known as the Combined method. This method utilizes data from FSTT tables as well as

knowledge of facial muscle anatomy (3, 47).

FSTT data are the foundation of most methods of facial reconstructions as they serve as

guidelines for establishing the facial contour of skulls. Therefore, it is important that FSTT

data be correctly quantified and standardized, so they are statistically reliable (12, 51-53).

Scientists have collected FSTT data over the years by a variety of means, ranging from old

techniques where a needle was used to puncture the skin of cadavers to take measurements,

to current non-invasive methods such as computed tomography (CT) and magnetic

resonance imaging (MRI) scanning (53, 54). When FSTT data are used in a facial

reconstruction, it is important that the practitioner select FSTT data that represent the whole

population best. A standard strategy in research is to use the arithmetic means of FSTT

datasets as the central tendency indicator (49). Over time, numerous studies have been

conducted that have collected FSTT data from various populations. In these studies, FSTT

data are usually stored in tables that are organized according to population, sex, age, and

body mass index (53). The data are arranged in this way as these factors directly influence

FSTT measurements, which means a practitioner needs to take this information into account

when selecting FSTT data. The relationship between the information gathered from a

biological profile, and FSTT highlights the importance of constructing a biological profile,

following the discovery of unknown human remains.

Recently the validity of FSTT data was brought into question by Herrera (53), as mathematical

and statistical inconsistencies in previous literature were revealed to be a result of poor

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methodological practice and small sample sizes. In response, researchers developed methods

to improve the accuracy of using the arithmetic means as the central tendency indicator by

adjusting the statistical method by which FSTT data are selected, with an overall aim to

increase the reliability of facial reconstructions. A study done by Stephan (51) revised FSTT

data from 2008 onwards to solidify that T-Tables were still the optimal standard for

identifying the arithmetic mean of FSTT tables. The law of large numbers and central limit

theorem suggest that as a dataset increases in size it will more accurately represent the

group as a whole, and therefore Stephan (51) suggested the inaccuracies associated with

FSTT data will be reduced as more FSTT data had become available. Between 2008 and 2014

Stephan (51) discovered the differences in arithmetic mean between new larger datasets and

previous datasets was negligible, suggesting that T-Tables are still suitable for selecting FSTT

data.

In 2013 Stephan et al. (55) developed two methods for calculating the arithmetic mean of

skewed datasets to represent the groups best. These were the shorth (the mean of the

densest half of the data) and the 75-shormax (the 75th percentile between the short and the

maximum value) methods. The identification of the central tendency of FSTT data was

improved, however, to obtain the measurements required for these methods, raw data are

needed. As a result, FSTT data calculated using the shorth and 75-shormax technique

currently have small sample sizes, and it is therefore still practical to utilize T-Tables to obtain

the arithmetic mean (51, 56).

As well as FSTT, facial sensory organs (eyes, nose, mouth, nose, and ears) are very important

elements in facial reconstructions, and it is often quite difficult to estimate their appearance,

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location, and size (52). A study conducted by Herrera (47) described and cited methods

developed by various authors used to predict the appearance and location of facial organs

(refer Appendix 1). As the nature of these features cannot be solely predicted through

markers on the skeleton, there is known to be a degree of subjectivity in these methods

(Appendix 1). The terminus of facial reconstructions is to use specific guidelines and careful

analysis of the skull to estimate the most likely antemortem representation of osseous

remains (47, 48, 57). SWGANTH (24) protocol states three criteria that facial reconstructions

should aim to achieve. These include: (i) estimate antemortem facial appearance; (ii) suggest

the identity of the individual; and (iii) capture public attention. Facial reconstructions alone

are not a tool for identification; they are a tool of recognition and investigative teams

regularly achieve identification when the resulting images of the deceased individual are

used in a publicity campaign aimed to jog the memories of members of the public (52).

As discussed, when standard channels of inquiry fail to achieve identification, forensic facial

reconstructions are a powerful tool that can be used to significantly increase the chances of

identifying the remains of an unknown individual (3, 12, 48). The importance of facial

reconstructions can be seen in investigative processes, and humanitarian work, such as in

disaster victim identification (DVI) and its utility extends beyond the field of forensic science

to archaeology, where it is used for the identification of historical remains (48). Literature

covers a variety of techniques used to perform facial reconstructions that are often

distinguished from one another according to space (2D or 3D), tools (pencils, modeling clay,

or computer), and method adopted (47). Recently an opinion has formed amongst

researchers that suggests that the manual methods of facial reconstructions are no longer

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suitable in an investigative context as the process relies too heavily on subjective decisions

and artistic judgment (12, 47).

Anthropometry:

Craniofacial anthropometry is an important aspect of generating facial reconstructions.

Anthropometric measurements provide practitioners with a consistent system of

measurement which enables them to objectively describe a patient’s phenotype while also

generating a list of individualizing anomalies (58). There are a variety of methods available

that practitioners can utilize to quantify craniofacial surface morphology, which includes

traditional methods such as direct anthropometry and 2D photogrammetry, and new indirect

methods such 3D photogrammetry and 3D laser scanning (59). Anthropometric

measurements allow forensic anthropologists to construct biological profiles with accurate

metric data and determine features of an individual that could later be used to attain

identification.

There are a multitude of craniofacial landmarks that can be utilized in craniofacial analysis

(60) (Figure 1.) It is the ubiquitous nature of human cranial anatomy, that enables facial

reconstructions to be used as an identification method in forensic science (61). Accurate

craniofacial measurements enable a forensic anthropologist to determine the ancestry of

unknown osseous remains and establish a foundation on which FSTT measurements can be

selected and placed. The new indirect methods of obtaining craniofacial anthropometric data

obtain accurate results as 3D models can be rotated in a virtual space to allow easy access to

the landmarks being measured (58). In a study conducted by Fourie (59) two methods of

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indirect anthropometry were used, namely laser scanning and 3D photogrammetry, and they

were shown to yield very accurate measurements as the error for each method was under

one millimeter. The aim of developing new technology to obtain accurate craniofacial

measurements is to solidify anthropometric evidence in court, since as for the technique to

be scientifically defensible, the reliability of these methods must be demonstrated through

large databases with statistical stability (61).

Figure 1. Craniometric landmarks for 3D analysis. Definitions are provided in Appendix 2. a Norma Lateralis; b Norma Frontalis; c Norma Medialis; d Norma. From (60).

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New Technology and Facial Reconstructions:

The recent development of 3D and CAD has had significant application to forensic science as

it has allowed the process of facial reconstructions to be completed in a virtual space. The

process requires the rendering of a 3D model of a deceased individual’s skull, which can be

achieved using photography techniques and laser or CT scanning, followed by importing the

model into 3D/CAD modeling software where a practitioner can model and layer soft tissue.

The aim of this is to increase the objectivity of the process and allow the possibility of it to be

standardized (12, 52). With a reduction in measurement inaccuracies and errors associated

with the current manual methods, this process should increase the value of facial

reconstruction evidence in court as the reliability of the method can be statistically defined

(12).

Research conducted by Lee (54) considered determining the accuracy of 3D facial

reconstruction processes by comparing the results with the faces of the participants of the

study. The 3D model skulls in this study were generated using CT scans and were augmented

in Geomagic Freeform Plus software. This was a blind study where the authors only knew the

ancestry of the participants. Information obtained from constructing a biological profile was

used to estimate FSTT, and this was applied to the 3D model using the Combination method

(Figure 2). The comparison stage of this study involved importing both the facial

reconstruction and CT scans of the subject’s faces into Geomagic Freeform Plus software

where they were overlaid and aligned using facial landmarks which highlighted the

discrepancies between the two 3D models. This study successfully demonstrated that

updated FSTT data can be used in facial reconstructions and can facilitate its reliability

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through quantitively accurate results. It should be noted that only three subjects (two males

and one female) were used in this study and they were all of Korean descent. Additionally,

facial components were predicted using guidelines from European ancestry groups which

were deemed inappropriate by the authors. Therefore, further studies should develop

prediction protocol for facial components for specific ancestral groups.

Figure 2. 3D computerized facial reconstruction procedure following the Combination method. From (54).

While new technology has allowed the facial reconstruction process to occur within a virtual

space, elements of the traditional manual methods are still utilized. In a study conducted by

Herrera (47), the skulls of two subjects (one male and one female) were used to compare the

difference of two manual methods, the American method, and the Combined method. The

authors were unaware of the identity of the individual, and FSTT data were obtained through

MRI scanning. The authors each generated facial reconstructions using both methods (four

facial reconstructions in total). The results of this study demonstrated that the American

method represented the female subject better while the Combination method was better

suited for the male subject. A possible explanation for this is that, as described above, the

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Combination method utilizes knowledge of facial muscle and FSTT, and males have stronger

facial muscles (3, 47). It was noted that the time taken to conduct the American method and

Combined method facial reconstructions were similar. While the sample size used was small,

this study indicates that some skulls have a propensity to be represented more accurately by

specific facial reconstruction methods.

CT and MRI technology is often the standard method of obtaining the data required to

generate a 3D model of a subject’s skull. In the studies described (47, 54) the data were

retrieved from subjects that were living. Most of a forensic anthropologists work requires the

identification of deceased victims meaning that CT and MRI technology are not the only

option to obtain data of the skull to create a 3D representation with (1, 2). With the

accelerated development of imaging technology, it is now possible to efficiently obtain high-

resolution images of osseous remains that can be impactful in an investigative context (62). A

study by Jo (62) indicated that imaging devices now can catalog macro- and micro-level

features of forensically relevant objects, such as a skull, and consequently has led to the

ability to store detailed data photographically.

Photogrammetry:

Photogrammetry is a photography technique that can be utilized to determine the shape and

location of an object using one or more photographs of that object. It employs methods of

image measurement, and can be applied to any situation in which an object is able to be

photographically recorded (63). The primary purpose of this technique is to generate a 3D

reconstruction of an object or area using a central projection imaging mathematical model

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that generates accurate reproductions in a virtual space. In simple terms photogrammetry

can measure and represent 3D objects using data that is stored in 2D photographs (Figure 3).

This technique has a wide scope of application such as in deformation surveys, modeling of

buildings, medicine and engineering, accident reconstructions, and crime scene

investigations (64). Additionally, it is also utilized in sectors of industry such as in Unmanned

Aerial Vehicles (UAV) where it is used to map regions of land for earthwork projects (63-65).

Photogrammetry is a non-contact technique which is a desirable quality in a forensic context

as this prevents evidence from being disturbed (5-9, 64, 66). This technique also enables the

storage of information allowing it to be re-assessed at any time which is favorable in a

forensic context where examination times may take place a period of time after the

discovery of evidence (64).

Figure 3. Principle of photogrammetric measurement. From (63).

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Close Range Photogrammetry

Close range photogrammetry (CRP) is a technique that is effective in documenting small

objects in detail (9). In an investigative context, this technique is widely applicable and is

therefore frequently used by law enforcement to aid in traffic accidents and crime scene

measurements (10). CRP is a relatively low cost method of storing data as it only requires a

camera and photogrammetry software, however Osman and Tahar (64) suggests that this

method is best applied with a high quality digital camera. CRP is also very accurate, Luhmann

et al. (63) noted that inaccuracies ranged from 0.1mm to 1cm on objects between 1m to

200m in size (64). To achieve the best results, it is favorable that a practitioner follows

specific protocol for photographically documenting forensically relevant objects as this will

greatly affect the accuracy of the resulting 3D output (6, 10). Fortunately, is it standard

practice in a forensic investigation to photograph objects with a scale present as this is

required in photos to accurately measure the forensically relevant objects using CRP. A

critical logistic advantage of this technique is that a trained practitioner is not required on

scene, the method of capturing photographs for photogrammetry purposes can be explained

to and performed by personnel already present at the scene (5). CRP also offers an extremely

effective way of presenting evidence in court as objects can be displayed in 3D with detail

and can be moved and rotated to be easily visualized by a jury which could not be achieved

using 2D photographs.

Between 1997 and 2003 several authors published a range of research relating CRP to

forensically relevant objects for comparative purposes (5-9). The earliest article published by

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Brüschweiler et al. (66) considered how CRP could be used to investigate injuries to skin, soft

tissues, and bones. This technique allowed the authors to overlay and compare injured

surfaces to objects in a 3D/CAD environment that may have caused the injuries (Figure 4).

Brüschweiler et al. (9) later expanded on this research and developed a manual for the

documentation process of forensically relevant objects using CRP. They demonstrated the

need for a new approach of photographing objects by taking a series of photos to capture

the amount of detail necessary for a comparative analysis. The authors noted that a point in

space is defined by the intersection of three vectors and so must be photographed from at

least three angles, they also suggested that a series of photos from a fourth angle

(perpendicular) should be captured to increase accuracy. Brüschweiler et al. (9) also

demonstrated that initially an object is defined as a cluster of points in virtual space which

grows in density depending on the number of reference points on that object, resulting in

objects of complex shape being represented as a dense point cloud. When the points are

connected by lines the point becomes a mesh which more clearly represents the forensically

relevant object. Since this research the foundation of the photogrammetry method has

remained unchanged, however with higher quality cameras and new photogrammetry

software it has allowed more flexibility with the series photography protocol.

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Figure 4. 3D visualization of the match and the impact angle of the of the injury-causing tool with the two impression fractures. From (7).

There is a multitude of methods of wound documentation that have been published by

dermatologists and forensic pathologists (7). Currently the standard procedure in forensic

medicine for documenting injuries inflicted upon a deceased person’s body remains to be

photography with precise measurements (7). The disadvantage of this process is that

photography reduces a 3D wound to a 2D level which limits a person’s ability to accurately

visualize and compare a wound to possible implements that caused it. This is because

reducing a 3D object to a 2D photograph can distort its shape and therefore lead to

misinterpretation. Photographic documentation, when utilized with forensic CAD-supported

photogrammetry, is in a stage of growth in the field of forensic medicine. Studies have shown

that CRP is a powerful tool that can be used for patterned wound and weapon analysis, and

identification (5). It was found that in comparisons of patterned injuries and injury-causing

implements that even though there can be a disparity in size between identifying features a

physical match can still be attained by means of CRP. Thali et al. (8) also applied CRP to

bitemark documentation (Figure 5). Currently, the traditional documentation method for bite

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mark analysis includes, colour, black and white, ultraviolet, and infrared photography, with

additional swabbing of DNA. The authors found that they could identify the topographical

features of each individual tooth which could potentially lead to an identification of the

individual who bit the victim.

Figure 5. The bite mark and the digitized casts of the suspects were examined with respect to matching shapes, angles and dimensions in the 3D. From (8).

In 2016 Becker (12) demonstrated that a 3D representation of a skull can be generated using

CRP. He was then able to use open-source software to conduct a facial reconstruction in

virtual space. While the method he used was not explicitly stated, it was mentioned that

Blender® has the capacity to store saved muscles that could be used later, suggesting the

Russian or Combination method was used. The software used to generate the 3D model of

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the skull was Virtual Structure from Motion® and MeshLab® and, when all the steps of the

process of generating a facial reconstruction were combined, it took no longer than 20 hours

to complete. As this study was a case study, Becker (12) had access to information regarding

the female victims’ biological profile, FSTT data and an antemortem image. A high-quality

digital camera was used in this study as well as the camera of an Apple iPhone 4, however it

was noted that only the digital camera photos were of high enough quality to execute the

method. Becker (12) found that the method could successfully represent the deceased

individuals antemortem appearance with minimal error. Scientific literature has therefore

proven that FSTT data can be used to accurately estimate an individual’s appearance and

that facial reconstructions can be conducted in a virtual space. What it has not addressed is if

accurate facial reconstructions using FSTT data estimations can be conducted in a virtual

space. This is what this research aims to address.

Local Applications:

Remote regions of Western Australia are common areas for discovering osteological remains.

After the discovery, the police are often contacted, resulting in general duties (GD) police

officers attending the site. GD police officers often seek advice to ensure they capture

detailed photographs of the remains which they can then digitally transfer to a forensic

anthropologist. The forensic anthropologist will then be able to identify the species of the

remains, and in some cases their significance. For example, in Australia, there is significance

in discovering remains of Australian Aboriginals as it can define an area of land as an

Aboriginal site or territory that is significant according to Aboriginal tradition. The Aboriginal

Heritage Act 1972 prevents further disruption of the land (e.g., mining) until the Minister of

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Aboriginal Affairs provides the authority to do so. If the remains contain historical

significance or damage that warrants further analysis, a forensic team will be sent to the site

to acquire the remains. The identification of osteological remains is often achieved later

using radiography, DNA analysis, analysis of dental records, or facial reconstructions (3).

With the recent development and documented success of CRP it would be possible for law

enforcement to begin and strengthen their efforts of identification at an earlier stage of

investigations. With new photogrammetry software, it would be possible for an expert to

describe the specific method of photography required to properly document a forensically

relevant object, such as a skull. These photographs would then be digitally transferred to a

forensic anthropologist or individual with sound knowledge of human cranial anatomy and

the morphological variation that exists between human populations to construct a 3D

representation of the discovered skull. After the application of precise craniofacial

anthropometric measurements to determine ancestry, the selection of FSTT data and

modeling of facial organs can be done to generate accurate facial representation of the

unknown remains using population data. These facial reconstructions can then be utilized in

a publicity campaign by law enforcement to jog the publics memory and potentially lead to

the identification of the remains through a low-cost and efficient method.

Broad Applications and the Future:

As discussed above photogrammetry has a wide scope of application. With the development

of drone technology aerial photogrammetry could become a method of capturing large

outdoor crime scenes in an efficient manner (10). This could become a streamlined process

as drones have access to global positioning systems (GPS) which would make the mapping

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process automated. A review by Colwill (10) noted that aerial drone photogrammetry has

been utilized by companies to scan areas such as farmland, golf courses, and historic

buildings however it does not appear to have been utilized in a forensic context.

The development of non-direct methods of craniofacial anthropometry has led to an increase

in the accuracy of craniofacial measurements. Mallett (61) noted that due to the ubiquitous

nature of the face, anthropometric measurements may potentially surpass dermatoglyphic

fingerprints, dental analysis, and DNA in being a remotely detectable method of

identification. Due to the advancement in mobile camera technology, evidence containing

the face of individuals suspected of a crime is becoming far more common in investigative

contexts. Currently accurate facial recognition is a challenging issue as the quality of material

that contains facial information is usually poor (67-70). It is possible that the future direction

of identification will move towards identification though biometrics as the quality of facial

material increases and the accuracy of craniofacial anthropometry increases.

Being able to manipulate 3D representations of objects in a virtual space is an extremely

powerful tool in an investigative context. When forensically relevant objects are

photographed and represented in a 2D format important information can be lost. There is a

significant amount of literature which demonstrates that comparisons of 3D objects can yield

valuable information and can be used for demonstrative purposes in a courtroom. It is likely

that in the future digitally storing forensically relevant objects will be standard practice in an

investigative context, additionally it is likely that evidence will be presented in a 3D format.

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EXPERIMENTAL AIMS AND DESIGN

Aims and Objectives:

The aim of this research is to develop an efficient method of generating 3D facial

reconstructions using photogrammetry, and open-source 3D/CAD software. To achieve this

several objectives must be completed, and these are listed below.

1. To generate an accurate 3D representation of a model skull utilizing CRP.

2. To classify the ancestry of a model skull using open-source 3D/CAD software by

identifying and measuring anthropometric craniofacial landmarks used in ancestry

identification.

3. To generate several facial reconstructions of different races using FSTT data based on

the ancestral classification of the model skull.

Experimental Design:

Photogrammetry

A 3D representation of a model skull will be generated using AgiSoft Photoscan® software.

The protocol required to photographically document, and process the model skull within the

software will be followed as per the AgiSoft Photoscan® User Manual, Professional Edition,

Version 1.3. Photographs will be taken with a Nikon D5500 SLR camera in an area with

diffused light to reduce shadowing. A black background will be used in accordance with the

methodology of Becker (12). The resulting 3D model of the skull will then be exported in a

format that is compatible with 3D/CAD software.

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3D/CAD classification and modeling

The 3D representation of the model skull will be imported into Blender® V2.78 (3D/CAD

software). Within Blender® V2.78, craniofacial landmarks and structures used to classify

ancestry will be identified, marked and measured. Data from studies that have identified the

average FSTT of soft tissue landmarks will be used to generate facial reconstructions

representing the different races the model skull may be, due to its ancestral classification.

The arithmetic mean of FSTT data will be identified using T-Tables in accordance with the

results of (51).

Experimental Design

The model skull has a complete history of the individual the skull was modeled from,

including photographs of when they were alive. A blind study will be undertaken where 3D

facial reconstructions will be generated and compared to both the photographs of the

deceased and a facial reconstruction generated using the traditional manual method.

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CONCLUSION

The construction of a biological profile is an important task a forensic anthropologist must

complete after the discovery of osseous remains. This literature review covered a variety of

methods available that can be used to obtain information of the individual’s ancestry, sex,

age, and stature. Literature containing population information regarding the FSTT often

stores FSTT measurements by these categories as there is a strong relationship between the

two. FSTT measurements are the foundation of generating facial reconstructions as they

establish the lines and contours of the face. Unfortunately, it was realized that poor

methodological practice and small sample sizes lead to the arithmetic mean of FSTT datasets

not accurately representing the average of the population from which they were derived.

This led researchers to develop methods to overcome skewed datasets to accurately identify

the central tendency indicator. While these methods were successful, raw data are required

to calculate the new averages resulting in corrected FSTT datasets having even smaller

sample sizes than before. There it should be noted that the accuracy of using FSTT data to

generate facial reconstructions will become more accurate as the datasets are built.

Scientific literature covers a range of uses of CRP within forensic science that includes but is

not limited to the comparison of bone injuries to injury-causing implements and bite marks in

skin to dentition. CRP offers many benefits in an investigative context as the method of

photography required to capture can be described to a law enforcement officer already

present at the scene of a crime. Additionally, CRP is a non-contact method of cataloging

evidence, which is desirable in forensics as it greatly reduces the chances that items of

evidence will be disturbed or contaminated. This is of use in Australia as osseous remains are

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often discovered in remote locations. A photogrammetry expert or a forensic anthropologist

with the knowledge of photogrammetry software would then be able to generate a 3D

representation of the skull of the unknown remains.

Due to the recent development of 3D/CAD software, craniofacial anthropometric

measurements have seen an increase in accuracy. This has allowed practitioners to identify

the ancestry of remains using 3D represented crania. Research has led to the development of

various methods of facial reconstructions which utilize knowledge of FSTT and facial muscles

to generate an antemortem representation of a deceased individual. Scientific literature has

proven that it’s possible to generate a facial reconstruction in virtual space and that FSTT

datasets can accurately represent an individual’s antemortem appearance. This research will

address the use of FSTT datasets to generate facial reconstructions in a virtual space where

the identity of the individual is not known. Successful results will lead to the development of

a streamlined process that law enforcement can utilize to achieving identification in early

stages of investigation.

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REFERENCES

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14. Sholts SB. Identification of Group Affinity from Cross-sectional Contours of the Human Midfacial Skeleton Using Digital Morphometrics and 3D Laser Scanning Technology* HUMAN MIDFACIAL CONTOUR ANALYSIS. Journal of forensic sciences. 2011;56(2):333-8. 15. Krogman WM. The human skeleton in forensic medicine1962. 16. Coleman DA. People and Races. Population Investigation Committee; 1979. p. 379-80. 17. Stewart TD. Essentials of Forensic Anthropology. Thomas CC, editor. IL: Springfield; 1979. 18. Krogman WM, İşcan MY, Steyn M. The human skeleton in forensic medicine. Third ed. Springfield, Illinois, U.S.A: Charles C Thomas Publisher, LTD; 2013. 19. Phenice TW. A newly developed visual method of sexing the os pubis. American journal of physical anthropology. 1969;30(2):297. 20. Klales AR, Ousley SD, Vollner JM. A revised method of sexing the human innominate using Phenice's nonmetric traits and statistical methods. American Journal of Physical Anthropology. 2012;149(1):104-14. 21. Buirkstra J, Ubelaker D. Standards for Data Collection from Human Skeletal Remains. AR: Fayetteville; 1994. 22. Bass W. Human Osteology: A Laboratory and Field Manual. Columbia, MO: Missouri Archaeological Society; 2005. 23. White TD, Folkens PA. The human bone manual. Amsterdam;Boston;: Elsevier Academic; 2005. 24. SWGANTH. Scientific Working Group of Forensic Anthropologists. In: Laboratory DoDCI, editor. U.S. Department of Justice2008. 25. Moorrees CF, Fanning EA, Hunt JEE. Age Variation of Formation Stages for Ten Permanent Teeth. Journal of dental research. 1963b;42:1490. 27. Agisoft PhotoScan User Manual: Standard Edition, Version 1.3. Agisoft LLC; 2017. 28. Schour I, Massler M. The Development of Human Dentition. American Journal of Physical Anthropology. 1941;63(1):39-56. 29. Ciapparelli L. The Chronology of Dental Development and Age Assessment. Clark D, editor. Oxford, England: Wright; 1992.

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30. Hoffman JM. Age estimations from diaphyseal lengths: two months to twelve years. Journal of forensic sciences. 1979;24(2):461. 31. Scheuer L, Black S. Developmental Juvenile Osteology. London, U.K.: Academic Press; 2001. 32. Garvin HM, Passalacqua NV. Current Practices by Forensic Anthropologists in Adult Skeletal Age Estimation. Journal of Forensic Sciences. 2012;57(2):427-33. 33. Todd T. Age Changes in the Pubic Bone: I, the White Male Pubis. American Journal of Physical Anthropology. 1920;3:286-334. 34. Iscan MY, Loth SR, Wright RK. Age estimation from the rib by phase analysis: White females. Journal of Forensic Sciences. 1985;30(3):853-63. 35. İşcan MY, Iscan MY, Loth SR, Loth SR, Wright RK, Wright RK. Metamorphosis at the sternal rib end: A new method to estimate age at death in white males. American Journal of Physical Anthropology. 1984;65(2):147-56. 36. Lovejoy CO, Meindl RS, Meindl RS, Pryzbeck TR, Pryzbeck TR, Mensforth RP, et al. Chronological metamorphosis of the auricular surface of the ilium: A new method for the determination of adult skeletal age at death. American Journal of Physical Anthropology. 1985;68(1):15-28. 37. Murray KA, Murray T. A test of the auricular surface aging technique. Journal of Forensic Sciences. 1991;36(4):1162-9. 38. Shirley NR, Jantz RL. Spheno-Occipital Synchondrosis Fusion in Modern Americans: BASILAR SYNCHONDROSIS. Journal of Forensic Sciences. 2011;56(3):580-5. 39. Lottering N, MacGregor DM, Alston CL, Gregory LS. Ontogeny of the spheno-occipital synchondrosis in a modern Queensland, Australian population using computed tomography: Recalibrated Standards In Spheno-Occipital Synchondrosis Fusion. American Journal of Physical Anthropology. 2015;157(1):42-57. 40. Dwight T. Methods of Estimating Height From Parts of The Skeleton. Med Rec. 1894;46:293-6. 41. Raxter MH, Auerbach BM, Ruff CB. Revision of the fully technique for estimating statures. American Journal of Physical Anthropology. 2006;130(3):374-84. 42. Wilson RJ, Herrmann NP, Jantz LM. Evaluation of stature estimation from the database for forensic anthropology. Journal of Forensic Sciences. 2010;55(3):684-9.

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43. Ubelaker DH. A history of Smithsonian--FBI collaboration in forensic anthropology, especially in regard to facial imagery. Federal Bureau of Investigation; 2000. 44. Ubelaker DH, Bubniak E, O'Donnell G. Computer-assisted photographic superimposition. Journal of Forensic Sciences. 1992;37(3):750-62. 45. Brogdon BG. The scope of forensic radiology. Clinics in laboratory medicine. 1998;18(2):203. 46. Pfaeffli M, Vock P, Dirnhofer R, Braun M, Bolliger SA, Thali MJ. Post-mortem radiological CT identification based on classical ante-mortem X-ray examinations. Forensic Science International. 2007;171(2):111-7. 47. Herrera LM. Comparison Among Manual Facial Approximations Conducted by Two Methodological Approaches of Face Prediction. Journal of forensic sciences. 2017. 48. Wilkinson C. Facial reconstruction - anatomical art or artistic anatomy? Journal of anatomy. 2010;216(2):235-50. 49. Parks CL. Application of Enhanced Point Estimators to a Sample of CT-derived Facial Soft Tissue Thicknesses. Journal of forensic sciences. 2017;62(4):994-7. 50. Parks CL, Richard AH, Monson KL. Preliminary assessment of facial soft tissue thickness utilizing three-dimensional computed tomography models of living individuals. Forensic science international. 2014;237:146.e1-.e10. 51. Stephan CN. The Application of the Central Limit Theorem and the Law of Large Numbers to Facial Soft Tissue Depths: T-Table Robustness and Trends since 2008. Journal of forensic sciences. 2014;59(2):454-62. 52. Guyomarc'h P, Dutailly B, Charton J, Santos F, Desbarats P, Coqueugniot H. Anthropological Facial Approximation in Three Dimensions (AFA3D): Computer‐Assisted Estimation of the Facial Morphology Using Geometric Morphometrics. Journal of Forensic Sciences. 2014;59(6):1502-16. 53. Herrera LM. Forensic facial approximation assessment: can application of different average facial tissue depth data facilitate recognition and establish acceptable level of resemblance? Forensic science international. 2016;266:311-9. 54. Lee W-J. Correlation Between Average Tissue Depth Data and Quantitative Accuracy of Forensic Craniofacial Reconstructions Measured by Geometric Surface Comparison Method. Journal of forensic sciences. 2015;60(3):572-80.

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55. Stephan CN, Simpson EK. Facial Soft Tissue Depths in Craniofacial Identification (Part I): An Analytical Review of the Published Adult Data. Journal of Forensic Sciences. 2008;53(6):1257-72. 56. Stephan CN, Simpson EK, Byrd JE. Facial Soft Tissue Depth Statistics and Enhanced Point Estimators for Craniofacial Identification: The Debut of the Shorth and the 75‐Shormax. Journal of Forensic Sciences. 2013;58(6):1439-57. 57. Wilkinson C. Facial identification of the dead. Journal of Anatomy. 2008. 58. Farkas LG, Deutsch CK. Anthropometric determination of craniofacial morphology. American journal of medical genetics. 1996;65(1):1-4. 59. Fourie Z. Evaluation of anthropometric accuracy and reliability using different three-dimensional scanning systems. Forensic science international. 2011;207(1-3):127-34. 61. Mallett XDG. An Exploration of Sample Representativeness in Anthropometric Facial Comparison* ANTHROPOMETRIC FACIAL COMPARISON. Journal of forensic sciences. 2010;55(4):1025-31. 62. Jo J. 3D facial shape reconstruction using macro- and micro-level features from high resolution facial images. Image and vision computing. 2017;64:1-9. 63. Luhmann T, Robson S, Kyle S. Close Range Photogrammetry : Principles, Techniques and Applications. Dunbeath, UNKNOWN: Whittles Publishing; 2006. 64. Osman MR, Tahar KN. 3D accident reconstruction using low-cost imaging technique. Advances in engineering software (1992). 2016;100:231-7. 65. Siebert S, Teizer J. Mobile 3D mapping for surveying earthwork projects using an Unmanned Aerial Vehicle system. Automation in construction. 2014;41:1-14. 66. Brüschweiler W, Braun M, Fuchser HJ, Dirnhofer R. Photogrammetrische Auswertung von Haut- und Weichteilwunden sowie Knochenverletzungen zur Bestimmung des Tatwerkzeuges — grundlegende Aspekte. Rechtsmedizin. 1997;7(3):76-83. 67. Bruce V, Healey P, Burton M, Doyle T, Coombes A, Linney A. Recognising Facial Surfaces. Perception. 1991;42(11):1200-14. 68. Jiang D, Hu Y, Yan S, Zhang H. Efficient 3D reconstruction for face recognition. Pattern recognition. 2005;38(6):787-98. 69. Choi K, Toh K-A, Byun H. Realtime training on mobile devices for face recognition applications. Pattern recognition. 2011;44(2):386-400.

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70. Mallett X, Eacute. Forensic Facial Comparison: Issues of Admissibility in the Development of Novel Analytical Technique. Journal of forensic sciences. 2013;58(4):859-65.

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APPENDICIES

Appendix 1. Guidelines for making facial approximations. From (47).

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Appendix 2. Craniometric landmarks including their abbreviations, definitions, and various

classifications. From (60).

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Part Two

Manuscript

Developing an Efficient Method for Generating Facial Reconstructions Using Photogrammetry and Open Source 3D/CAD Software.

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Developing an Efficient Method for Generating Facial Reconstructions Using Photogrammetry and Open Source 3D/CAD Software: A Preliminary Study.

Cahill Hunt1, John Coumbaros1,2, Brendan Chapman1

1Murdoch University, School of Veterinary and Life Sciences, Perth, WA.

2MForensic Science Laboratory, ChemCentre, Perth, WA.

ABSTRACT

The development of 3D technology and software has enabled the process of facial reconstructions to

occur within a virtual space. This study was aimed at using photogrammetry to develop an efficient

method of generating a 3D representation of a deceased individual’s skull that may be utilized by law

enforcement to aid in identification. A series of photographs (n=220) was taken with an Apple iPhone

7 camera and imported into AgiSoft Photoscan® Professional Edition, Version 1.3, to generate a 3D

model. Craniofacial landmarks (n=10) were marked on this model, and inter-landmark distances (ILDs)

(n=5) were measured using a preset scale as a reference measurement. Multiple p-values (where

p>0.05) were calculated using two-sample T-tests suggesting that the 3D model was accurate. This

study provides a practical and low-cost option for the first step in developing an efficient method to

perform facial reconstructions within a virtual space, with an aim to eliminate of subjectivity from the

process.

___________________________________________________________________________________

Keywords: Forensic Science, Photogrammetry, Forensic Anthropology, Facial Reconstruction,

Anthropometry, Skull, Craniofacial, Open-source Software, 3D

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Table of Contents

ABSTRACT ............................................................................................................................................... 61

List of Figures ......................................................................................................................................... 63

List of Tables ........................................................................................................................................... 63

List of Abbreviations ............................................................................................................................... 64

INTRODUCTION ...................................................................................................................................... 65

MATERIALS & METHODS ........................................................................................................................ 69

Photogrammetry ................................................................................................................................ 69

Metric Analysis ................................................................................................................................... 70

RESULTS .................................................................................................................................................. 72

DISCUSSION ............................................................................................................................................ 74

CONCLUSION .......................................................................................................................................... 80

REFERENCES ........................................................................................................................................... 81

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List of Figures

Figure 1: Known distances between two points (red tape) on three regions of the 3-

dimensional model skull generated using AgiSoft Photoscan® Professional Edition, Version

1.3. ................................................................................................................................................. 70

Figure 2: Rendered 3-dimensional model skull using AgiSoft Photoscan® Professional Edition,

Version 1.3, viewed from ten angles. ............................................................................................ 72

List of Tables

Table 1: Craniofacial landmarks used in metric analysis of the 3-dimensional model skull. ........ 71

Table 2: Comparison of measurements between physical and computerized craniofacial

landmark pairs and corresponding p-values obtained from multiple two-sample T-tests. ......... 73

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List of Abbreviations

___________________________________________________________________________________

CAD Computer Aided Design

CRP Close Range Photogrammetry

CT Computed Tomography

DNA Deoxyribonucleic Acid

FSTT Facial Soft Tissue Thickness

GD General Duty

ILDs Interlandmark Distances

L Left

MRI Magnetic Resonance Imaging

R Right

3D Three-dimensional

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INTRODUCTION

The task of identifying unknown human remains is critically important in society as it

facilitates the progression of investigations into suspicious deaths, it brings closure to the

families of the deceased, and it enables the settlement of legal issues, such as the succession

of property (1, 2). There are various methods forensic practitioners can utilize to ascertain

identification, and the most common of these include radiography, analysis of

deoxyribonucleic acid (DNA) or dental records, and facial reconstructions (3). Forensic

anthropologists can ascertain identification of human remains when they are skeletonized,

heavily burned, or the soft tissue has degraded to the point that other professionals, such as

forensic pathologists, cannot obtain information about the individual (1, 2). Forensic

anthropologists acquire a broad range of skills during their training which allows them to

identify the sociocultural and human skeletal biology determinants of human variation. Using

this knowledge forensic anthropologists can offer calculated insights into cases of suspicious

deaths, single and mass grave excavations, mass disasters, and human rights work (1, 2).

In investigations into suspicious deaths, building a biological profile is a crucial task forensic

anthropologists should endeavor to complete (1, 4). The information gathered from this

process often contributes to the resolution of medico-legal investigations as it reveals how

an individual may have lived, but also, the circumstances surrounding their death (2). The

construction of a biological profile involves identifying the ancestry, sex, age, and stature of

the deceased individual. There is a multitude of methods that can be utilized to obtain this

information, and the method utilized is often determined depending on the completeness of

the discovered osseous remains. If the information gathered from a biological profile does

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not initially lead to identification, there are different methods professionals can utilize to

achieve this. It is important to note that even though the information gathered from a

biological profile may not be directly responsible for the identification, forensic

anthropologists utilize it in the other methods they employ to attain identification.

The methods available to a forensic anthropologist that can be used to obtain an

identification include craniofacial superimposition, non-imaged records comparisons,

comparative radiography and facial reconstructions (1, 2). All these methods, except facial

reconstructions, require ante mortem information about the deceased person and therefore

when this information is not available, these methods cannot be performed. Additionally, it is

possible that in some situations, law enforcement has no suspicions of who the deceased

person may be. In these circumstances, a forensic anthropologist would perform a facial

reconstruction which an investigative team may use to achieve an identification of a

deceased individual (5). Facial reconstructions are often the last method used for

identification.

Historical methods of facial reconstructions have been associated with a degree of

subjectivity, whereby the soft tissue of the skull, and facial organs are represented with

artistic influence rather than being a purely objective representation generated from

statistically reliable data (6-8). Several well established manual methods exist that are

currently utilized to generate facial reconstructions; these are the American method, Russian

method, and combined method (9, 10). Ultimately, researchers aim to eliminate all

subjectivity from facial reconstruction methods and achieving this relies on utilizing modern

technology, and facial soft tissue thickness (FSTT) data (8). FSTT data serves as the guidelines

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for establishing the facial contour of skulls, and therefore it is important that FSTT data are

correctly quantified and standardized, so the technique is statistically reliable (5, 10-12). The

advancement of three dimensional (3D) computer aided design (CAD) software has allowed

the facial reconstruction process to occur in virtual space using 3D scanning techniques such

as computed tomography CT and magnetic resonance imaging MRI (13, 14). However, these

techniques can be costly and impractical in a commercial setting.

The use of photogrammetry techniques has been successful in the field of forensics. The

primary purpose of this technique is to generate a 3D reconstruction of an object or area

using a central projection imaging mathematic model which generates accurate

reproductions in a virtual space (15, 16). Close range photogrammetry (CRP) is a technique

that is effective in documenting small objects in detail (17). The benefits of CRP are that it is a

relatively low-cost method and that it is very accurate (15, 16). Another advantage of this

method is that a practitioner isn’t required at the scene as the method of photography

required to capture the object or scene can be explained to general duties (GD) police

officers on site (17-20). The success of CRP has been demonstrated through investigating

injuries to the skin, soft tissues, and bones (21, 22). Additionally, CRP has been used for

patterned wound and weapon analysis and identification, patterned injuries and injury

causing implements, and for bite mark documentation (17-20).

Recently, a study conducted by Becker (5), demonstrated that a 3D representation of a skull

could be generated using CRP. Becker was then able to perform a facial reconstruction using

available information about the subject’s biological profile and FSTT data. The reconstruction

was conducted within the open-source 3D modeling software Blender® where Becker was

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able to generate a facial representation that accurately replicated the ante mortem

appearance of the deceased individual. While Becker demonstrated that the process of facial

reconstructions could occur within a virtual space, it is rare in an investigative context that

the antemortem information Becker had access to in his study would be available to a

forensic anthropologist. Anthropometric measurements allow a forensic anthropologist to

construct a biological profile using accurate metric data which can later be used to obtain an

identification. In a study conducted by Fourie (23), photogrammetry was shown to yield

anthropometric measurements, with errors under one millimeter.

Therefore, the aim of this research was to generate a 3D representation of a skull modeled

from a deceased individual and validate its accuracy. The low-cost imaging technique of

photogrammetry was utilized as an alternative to expensive, and impractical CT and MRI

scanning methods and the accuracy of the 3D model was scrutinized by a 3D craniometric

analysis. The high rate of accuracy that photogrammetry has shown to have within the field

of forensics demonstrates the advantages it has for documenting small, forensically relevant

objects with a high degree of accuracy which could be beneficial to the process of 3D facial

reconstructions.

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MATERIALS & METHODS

The 3D model skull used in this research included a full report containing information

regarding the ancestry, age, sex and stature of the deceased individual the skull was modeled

from, as well as including antemortem images of the individual’s face. All photos in this study

were taken with an Apple iPhone 7 (Apple, California) camera (12-megapixels, and f/2.8

aperture). All 3D modeling was completed within AgiSoft Photoscan® Professional Edition

software. This software was installed on a machine running Windows 10 Home with 8GB of

memory, an Intel® Core™ i3-7100 CPU, and an AMD Radeon™ R5 M430 graphics card. All

physical measurements of the skull were performed with a LinearTools® digital caliper.

Photogrammetry

A 3D representation of a skull modeled from a deceased individual was generated with

AgiSoft Photoscan® Professional Edition software using 220 photographs. The protocol

required to photographically document and process the model skull was followed as per the

AgiSoft Photoscan® User Manual, Professional Edition, Version 1.3 with some differences.

The first alteration was made in accordance with the methodology from Brüschweiler et. al.

(21) where photographs were taken from three additional angles, as well as perpendicular to

the object as described in the AgiSoft Photoscan® User Manual. Additionally, a black

background was used in accordance with the methodology of Becker (5) to minimize the

interference of background during the process of alignment. To maintain constant lighting,

the photographs were taken in a room with multiple light sources that equally distributed the

light, and minimized the effects of shadowing. The skull was marked with six pieces of red

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tape (paired) approximately 0.5cm across, that were used to indicate three known

measurements (Figure 1). One set of the paired markers was used to establish a scale within

the AgiSoft Photoscan® software to enable computed measurements of distances between

craniofacial landmarks.

Figure 6: Known distances between two points (red tape) on three regions of the 3-dimensional model skull generated using AgiSoft Photoscan® Professional Edition, Version 1.3.

Metric Analysis

Various physical and computerized measurements were employed to assess the accuracy of

the 3D generated model skull. Within the AgiSoft® Photoscan Professional Addition software,

the measurement of the distance between the paired markers shown in Figure 1 (c) was

established as a reference scale. This scale was then used to measure the distance between

craniofacial landmarks within the software. Craniofacial landmark pairs were selected in

accordance with the methodology of Spradley and Jantz (24) with some differences.

Craniofacial landmarks were identified and marked on the 3D model using the “Create

Marker” tool within the AgiSoft Photoscan® software. Paired landmarks that could not be

(a) (b) (c)

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physically measured using digital calipers were not included. The craniofacial landmark pairs

that were used are presented in Table 1.

Table 3: Craniofacial landmarks used in metric analysis of the 3-dimensional model skull.

Triplicate measurements of both physical and computerized measurements were performed

and analyzed using multiple two-sample T-tests, evaluating the accuracy of the 3D digital

model of the skull compared to the physical model of the skull. All results were expressed as

an average of the triplicated measurements. P-values less than 0.05 were considered

statistically significant.

Paired

Alare L and R

Asterion L and R

Ectoconchion L and R

Mentale L and R

Zygomaxillary L and R

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RESULTS

Using photogrammetry, it was possible to construct an accurate 3D model of a skull. The

generated 3D representation shows a significant amount of detail when viewed from Norma

Frontalis (a), Norma Lateralis (c) and (g), Norma Occipitalis (e), and Norma Verticalis (i).

When viewed from Norma Basalis (j) a significant amount of information has been lost during

image processing (Figure 2).

Figure 7: Rendered 3-dimensional model skull using AgiSoft Photoscan® Professional Edition, Version 1.3, viewed from ten angles.

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The detail of the bone morphology and markings, allowed for the identification of the Alare

left (L) and right (R), Asterion L and R, Ectoconchion L and R, Mentale L and R, and Maxillary L

and R, which were utilized in the craniometric analysis of the 3D model skull.

Paired craniofacial landmark measurements showed the averages for the physical

measurements to be 0.0240 m, 0.1030 m, 0.1040 m, 0.0468 m, 0.0963 m, and the

computerized measurements to be 0.0240 m, 0.1000 m, 0.1050 m, 0.0456 m, 0.0977 m for

the Alare L and R, Asterion L and R, Ectoconchion L and R, Mentale L and R, and Maxillary L

and R, respectively. Multiple two-sample T-tests yielded p-values of 0.817, 0.139, 0.0992,

0.387, and 0.617 for the comparison between the Alare L and R, Asterion L and R,

Ectoconchion L and R, Mentale L and R, and Maxillary L and R, respectively. The data are

summarized in Table 2.

Table 4: Comparison of measurements between physical and computerized craniofacial landmark pairs and corresponding p-values obtained from multiple two-sample T-tests.

Paired Craniofacial

Landmarks

Physical

Measurement

Average (m)

Computerized

Measurement

Average (m)

p-value

Alare L and R 0.0240 0.0240 0.817

Asterion L and R 0.1030 0.1000 0.139

Ectoconchion L and R 0.1040 0.1050 0.099

Mentale L and R 0.0468 0.0456 0.387

Maxillary L and R 0.0963 0.0977 0.617

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DISCUSSION

An accurate 3D model was able to be generated using photogrammetry and AgiSoft

Photoscan® software. In this study 220 photographs were taken with an Apple iPhone 7

camera, which resulted in adequate coverage of the model skull while following the protocol

outlined in the AgiSoft Photoscan® User Manual, Professional Edition, Version 1.3. It is

noteworthy that the photography stage of this experiment showed there to be a degree of

flexibility in the image capturing process as the photographs were taken by hand and by

estimating by eye the amount of overlap that was required in subsequent photographs.

Photogrammetry typically requires forensically relevant objects to be photogrammetrically

treated where the object is to be photographed in series. In early studies conducted by Thali

and Brüschweiler a complex photogrammetry method was described that used the

conventional Rollie 3003 metric camera equipped with a f2.8/60mm macro-lens, and

required projection of light points onto the forensically relevant objects using an overhead

projector (17-21). This complex technique has become more straightforward as

photogrammetry software and camera technology has advanced. In previous studies

conducted by Colwill (25) using AgiSoft Photoscan® Professional Edition software Colwill

found that a detailed 3D representation of a singular outsole impression was able to be

generated using 16 photographs while the camera was set on automatic, and handheld. He

noted that the need for extensive quality controls in the imaging capturing process might not

be necessary if the final results are indistinguishable. Colwill (25) also noted that an increase

in the number of photos used to generate a 3D model may result in a more accurate

representation of a forensically relevant object that could be more readily measured.

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The documentation of forensically relevant objects has shown to be extremely successful in

generating a significant amount of detail that can utilized in many areas of forensic science.

In this study, the 3D model of a skull that was generated displayed a significant amount of

detail when viewed from Norma Frontalis, Norma Lateralis, Norma Occipitalis, and Norma

Verticalis. However, when viewed from Norma Basalis very little detail of cranial bone

morphology can be seen. This can be explained by the camera positions and the set-up of the

space where the photography took place. The model skull was placed on a stand that had a

square base with a cylindrical piece of wood that went through the foramen magnum

allowing the model skull to balance on top of it. The design of this stand made it difficult to

photograph the base of the skull resulting in poor photographic documentation. Complete

photographic documentation of Norma Basalis may be achieved by altering the design of the

stand, so the skull is elevated without obscuring any viewing angles.

The quality of the generated 3D model enabled the identification of craniofacial landmarks.

The craniofacial landmarks that were focused on in this study were adapted from the

methodology of Spradley and Jantz (24), a study in which standard and non-standard

craniofacial landmarks were compared to assess which set lead to better ancestry estimates

for the current American population structure. In this study, five pairs of the non-standard

set of craniofacial landmarks were used to create five Interlandmark distances (ILDs) that

could be measured using AgiSoft Photoscan® Professional Edition software. These ILDs were

selected as they were easily identifiable on the 3D model skull and they could be measured

physically on the model skull. These ILDs were not selected or utilized to estimate the

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ancestry of the deceased individual, only to provide straight line distances that could be used

to validate the accuracy of the generated 3D model skull.

To facilitate the measurement of ILDs, the skull was marked in accordance with the AgiSoft

Photoscan®, User Manual, Professional Edition, Version 1.3. Six small squares of red tape

were used to mark three random distances on the model skull. Only one of the pair of

markers was used to set a scale within the photogrammetry software as only one distance

between two points was required to utilize this tool. The distance, as depicted in Figure 1 (c),

was used for the scale, as the straight-line distance between points did not intersect the 3D

model of the skull. Once this distance was input into the software, ILDs could be measured by

the software based on the scale set using the physically measured distance.

Both the computed and physical measurements were performed in triplicates. Within the

AgiSoft Photoscan® software performing triplicate measurements involved removing all

craniofacial landmark markers and placing them onto the skull three times. As it is up to the

practitioner’s discretion as to where the landmarks are placed, human error was a factor that

needed to be considered. Two-sample T-tests were used to compare the averages of the sets

of computerized and physical measurements. All calculated p-values were greater than 0.05

suggesting that the null hypothesis was unable to be rejected. This meant that there was no

significant difference between the computerized and physical measurements, suggesting the

generated 3D model of the skull was accurate. In a previous study conducted by Becker (5), a

3D model of a skull was generated using the photogrammetry technique; however, Becker

had access to the biological profile of the deceased individual. The accuracy of the generated

3D model from this study would enable a forensic practitioner to identify the ancestry of the

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individual using craniofacial measurements, making this technique extremely beneficial in

investigative contexts where the deceased individual is unknown to the investigative team or

no ante mortem information of the deceased is available.

In Australia, osteological remains are often discovered in remote regions. After the discovery

of the remains, the police are often contacted, resulting in GD police officers attending the

site. GD police officers often seek advice to ensure they capture detailed photographs of the

remains which they can then digitally transfer to a forensic anthropologist. The forensic

anthropologist will then be able to identify the species of the remains, and in some cases

their significance. In Australia, there is significance in discovering remains of Australian

Aboriginals as it can define an area of land as an Aboriginal site or territory that is significant

according to Aboriginal tradition. The Aboriginal Heritage Act 1972 prevents further

disruption of the land (e.g. mining) until the Minister of Aboriginal Affairs provides the

authority to do so. In these circumstances, the identification of osseous remains is crucial to

the parties involved with the land. The success of the results of this research would allow GD

police officers to capture the skull photographically and digitally transfer the images to a

practitioner that would be able to generate an accurate 3D model of the skull for analysis at

low-cost.

There are significant advantages to this method in comparison to CT and MRI methods.

Photogrammetry offers significant logistical advantages as the equipment required to

capture the information is available in most law enforcement agencies and is extremely

portable. Additionally it should be noted that this study was conducted using the camera of

an Apple iPhone 7 which suggests that newer model phones could be used. In a study

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conducted by Becker (5), the camera of an Apple iPhone 4 was used however it was not

shown to be successful due to the low quality of the photos. If GD police officers have a

newer model phone camera with sufficient resolution this further drops the cost of this

method and increases the logistical advantage of portability. Additionally, with the

advancement of camera technology and photogrammetry software, there is a degree of

flexibility with the method of capturing photographs, meaning that a practitioner skilled in

photography may not be required at the scene as the general method of

photogrammetrically treating a forensically relevant object can be described to a GD police

officer.

This method of generating 3D models of deceased individual’s skulls using photogrammetry

shows significant promise in 3D facial reconstructions. When ante mortem data of the

deceased individual are not available to a forensic practitioner, a forensic anthropologist can

utilize craniofacial anthropometric measurements to objectively describe a deceased

person’s phenotype. This is normally done by physically measuring the distance between

craniofacial landmarks on the deceased individual’s skull; however, the success of this

research suggests that this may now be conducted within virtual space. Facial

reconstructions have previously been performed within virtual spaces using 3D/CAD

software, though in each case, antemortem data of the deceased individual has been

available to the practitioner (5, 14). With the significant detail of bone morphology that can

be seen on the 3D model created for this study, it would be possible to identify cranial

landmarks which can be used to determine the ancestry of the deceased and establish a

foundation on which FSTT measurements can be selected and placed. FSTT measurements

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are the foundation of generating facial reconstructions as they establish the lines and

contours of the face.

The future scope for this area of research would be to develop an efficient method of

generating facial reconstructions all completed within a virtual space. With the development

of software, it may be possible to automate a large part of the process including the

classification of ancestry using craniofacial landmarks measured within photogrammetry or

3D modeling software, the application of FSTT markers, and the layering of soft tissue onto

the 3D model skull within 3D modeling software. FSTT data could be sourced from existing

databanks of FSTT measurements of many populations depending on the ancestral

classification of the deceased individual. It has been the goal of researchers to eliminate all

subjectivity from this method of identification and base results on data that has a rigid

statistical foundation (8). This study provides a low-cost and efficient method of generating

an accurate 3D representation of a deceased individual’s skull which is the first step in

developing an efficient method to perform facial reconstructions within a virtual space.

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CONCLUSION

Facial reconstructions are the preferred method of identification that forensic

anthropologists use when very little antemortem data of the deceased individual exists, or

when law enforcement have no suspicions on who the deceased individual is. The

development and advancement of camera technology has led to the technique of

photogrammetry being utilized within the discipline of forensic science as a way of

documenting crime scenes and objects in 3D space. With the recent success of CRP as a

technique used to digitally document forensically relevant objects, this study aimed to

generate a 3D representation of a model skull and validate its accuracy. The successful

results showed that this technique is a very efficient and low-cost method of digital

documenting small objects. This study provides the first step in performing facial

reconstruction within a virtual space, and offers significant logistical advantages which would

enable law enforcement agencies to execute this form of identification quickly. Future

research should consider using these models to estimate ancestry, and use existing FSTT data

to generate facial approximations within 3D/CAD software.

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REFERENCES

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