Determination of Glycidyl Esters and 3-MCPD Esters in Edible Oils by

Sample Pretreatment with the Combination of Lipase Hydrolysis and

Modified QuEChERS for GC-MS Analysis

Hsin-Ya Tsai, Jhih-Ning Hsu, Chun-Jen Fang and Nan-Wei Su*

Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan

*Corresponding author:

*Nan-Wei Su, Ph.D., Professor

Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan

Tel: +886-2-33664806

Fax: +886-2-23632714

E-mail: snw@ntu.edu.tw

Figure S1. Chemical stability of glycidol in lipase reaction mixture during different time (0-2 h).

Data are triplicate determinations.10 mL Mcllvaine buffer (pH 7) spiked with glycidol at 1 mg/kg

was shaken vigorously at 1000 strokes/min at room temperature for 0-2 h.

Figure S2. Selective ion monitoring (SIM) chromatograms of (A) quantifier (m/z 44), and (B)

qualifier (m/z 31) of glycidol and (C) imposed SIM chromatograms of quantifier and qualifier of

glycidol at 0.02 mg/kg (LOD). SIM chromatograms of (D) quantifier, and (E) qualifier of glycidol

and (C) imposed SIM chromatograms of quantifier and qualifier of glycidol at 0.1 mg/kg (LOQ) in

oil matrix.

Figure S3. Calibration curves for glycidol and 3-MCPD in solvent

Figure S4. Selective ion monitoring (SIM) chromatograms of (A) glycidol (10 ppm) and (B) furfuryl

alcohol (internal standard, IS) (1 ppm) in solvent. Mass spectra of (C) glycidol and (D) furfuryl

alcohol in solvent.

Figure S5. Selective ion monitoring (SIM) chromatograms of (A) PBA derivatives of 3-MCPD (1

ppm) and (B) PBA derivatives of 3-MCPD-d5 (internal standard, IS) (100 ppb) in solvent. Mass

spectra of (C) PBA derivatives of 3-MCPD and (D) PBA derivatives of 3-MCPD-d5 in solvent.

Figure S6. Selective ion monitoring (SIM) chromatograms of (A) glycidol (0.5 mg/kg) and IS (1.0

mg/kg), (B) glycidol (1.0 mg/kg) and IS (1.0 mg/kg), (C) 3-MCPD (0.5 mg/kg) and IS (0.1 mg/kg),

(D) 3-MCPD (1.0 mg/kg) and IS (0.1 mg/kg) in oil matrix (2 g purified extra-virgin olive oil).