8/17/2019 Lesson Plan Project_Having Fun http://slidepdf.com/reader/full/lesson-plan-projecthaving-fun 1/11 CLASS/ TIME/ SUBJECT CONTENT IMPACT Year 3 Day 1 9.00 – 10.00 Engl!" Lang#age $%&#! ' Listening and Speaking T"e(e ' World of Knowledge T%)& ' Having Fun C%n*en* S*an+ar+ ' 1.1 By the end of the 6-year priary s!hooling" pupils will #e a#le to pronoun!e words and speak !on$dently with the !orre!t stress" rhyth and intonation. Learnng S*an+ar+ ' 1.1.1 %#le to listen to" say aloud and re!ite rhyes" tongue twisters and sing songs paying attention to pronun!iation" rhyth and intonation O,-e&*e! ' By the end of the lesson" pupils will #e a#le to& a' (dentify the people they !an see in the pi!ture. #' )espond to tea!her #y answering siple *uestions. A&**e! ' 1. Warers. +. ,ea!her asks the pupils to study the pi!ture. . ive tie to the pupils to dis!uss a#out the pi!ture. /. ,hey ask and answer to their friends. 0. upils and tea!her dis!uss a#out the pi!ture. 6. %sk a#out what they know a#out the persons. Tea&"ng a+!& ,e2t#ook" #la!k#oard" te2t#ook Tea&"ng an+ learnng !*ra*ege!' 1. upils know the pronouns #ased on their previous knowledge A++e+ al#e!' 3onte2tual learning. A!!e!!(en* ' E$LECTION %ttendan!e&44 5444 4445444 of the pupils a#le to a!hieve the o#e!tives 4445444 of the pupils a#le to answer the *uestions !orre!tly 4445444 of the pupils need e2tra guidan!e 4445444 of the pupils a#le to aster today7s lesson ,ea!her8s a!tion & 4444444444444444444444444 4444444444444444444444444 4444444444444444444444444 4444444444444444444444444 9 ,oday7s lesson will #e !arried forward due to 4444444444444444444444444 4444444444444444444444444 4444444444444444444444444 4444444444444444444444444 e(e+al Enr&"(en*

Determination of Folic Acid and its Related Substances of ... · ISSN 0326-2383 555 KEY WORDS: Content determination, Folic acid and related substances,HPLC. * Author to whom correspondence

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Page 1: Determination of Folic Acid and its Related Substances of ... · ISSN 0326-2383 555 KEY WORDS: Content determination, Folic acid and related substances,HPLC. * Author to whom correspondence

555ISSN 0326-2383

KEY WORDS: Content determination, Folic acid and related substances,HPLC.

* Author to whom correspondence should be addressed. E-mail: [email protected]

Latin American Journal of Pharmacy(formerly Acta Farmacéutica Bonaerense)

Lat. Am. J. Pharm. 31 (4): 555-60 (2012)

Regular ArticleReceived: February 4, 2012

Revised version: May 19, 2012Accepted: May 22, 2012

Determination of Folic Acid and its Related Substances of theFerrous Fumarate and Folic Acid Dispersible Tablet by HPLC

Q. CHEN, Y.L. LIU, Y.H. LUO*, M. LUO & L.Y. LUO

College of Pharmaceutical Sciences,Southwest University, Chongqing, China

SUMMARY. The aim of the present study was to establish a method for the determination of folic acid andrelated substances content in dispersible tablets. Method: A Shim-pack VP-ODS C18 reversed phase col-umn (4.6 mm × 250 mm) was used. The mobile phase consisted of methanol-phosphate buffer (20:80) witha pH of 6.3.The flow rate was 1.0 mL/min, and the detection wavelength was 277nm and the column tem-perature was 30 °C. Results: The calibration curve was linear in the range of 5~150 μg/mL (r = 0.9998) forfolic acid. The minimal detection limit was 99.08 %, n = 9 and the related substances were well separated.This method resulted to be convenient, accurate, selective and reliable, and can be applied for the qualitycontrol of folic acid.