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Detailed Dietary Fibre Comparisons for Fermentation & Microbial Stimulation

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happiGut™ – WHOLE PLANT / VIRGIN SUGARCANE FIBRE

Kfibre™ (happiGut™ ) is a natural whole-plant prebiotic fibre (sugar reduced) made of both soluble and insoluble fibres that are not isolated or purified. HappiGut™ possess the biochemical complexity of fruits and vegetable cellular materials that closely reflect the natural whole food products in contrast to fibre fractions of purified ingredients.

Kfibre™ (happiGut™) is prepared using technology that focuses on minimal processing and preserving the nutritional components of the source plant material—sugarcane. happiGut™ retains both soluble and insoluble fibre fractions that have higher and lower fermentable properties at ratios that more closely represent natural whole plant foods.

The complex biochemical fibre structure in happiGut™ supports stimulation of diverse microbiota and consequently enhanced production of major SCFAs – acetate, propionate, and butyrate. Its complex biochemical structure allows it to be fermented more uniformly along the entire colon length allowing to be progressively absorbed, unlike the isolated DF that gets used up in the fore gut/proximal section of the colon. This indicates the stimulation of microorganisms along the entire colon length leading to enhanced production of metabolites benefiting the whole colon.

happiGut™ is confirmed to stimulate microbial diversity (in-vitro and in-vivo) and to support the growth of beneficial bacteria including Bifidobacteria and Akkermansia

In addition to its DF content, happiGut™ is also confirmed to retain the inherent phytonutrients that are known to possess health effects including antioxidant flavonoids, chromium, tricin and silica.

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Insoluble & Soluble Prebiotic Fibre

HOW DOES happiGut™ STAND OUT?

ü Natural, complete whole-plant complex dietary fibre

ü Retains biochemical complexity of plant cellular materials that closely reflect those in natural whole fruits and vegetables in contrast to fibre fractions of purified ingredients

ü Fermented more uniformly along the entire colon length allowing to be progressively absorbed thus reducing the gas pressure that usually leads to bloating, abdominal pain, and indigestion.

ü Supports microbial diversity and consequently enhanced production of SCFAs along the entire gut length thus benefiting the whole gut.

ü Minimal “virgin” processing retains both soluble and insoluble fibre fractions that have higher and lower fermentable properties at ratios that more closely represent natural whole plant foods

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ü Made from virgin sugarcane fibre

ü Low heat, mechanical & water processing only

ü Supports digestive regularity & maintenance of intestinal health

ü Provides benefits of vital phytonutrients including flavonoids, chromium, tricin and silica

ü Low FODMAP & Gluten Free make HappiGut™ suitable for IBS & Coeliac users

Please read on to see our scientific comparison

of other fibre options

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INULIN – EXTRACT OF CHICORY ROOT (FOS/GOS)

Inulin is a soluble only, highly fermentable fibre. Fast-fermenting substrates may not provide as much SCFAs to the distal colon as slow-fermenting substrates. Inulin may be a trigger for FODMAP sensitivities or IBS, and specifically inulin sensitivity has been described.

Inulin does not accelerate gastric transit. In fact, higher doses ≥ 7g delays gastric emptying.

This delay in gastric emptying, together with a possible impairment of nutrient absorption in the small intestine may delay intragastric redistribution, which normally occurs as nutrients enter the duodenum.

Short-chain FOS/GOS (FOS - fructo-oligosaccharide / GOS - galacto-oligosaccharide) promotes only selective growth of specific microbiota e.g., Bifidobacteria. This study has shown that GOS/FOS only favours Bifidobacteria and reduce other specific butyrate-producing bacteria.

Fibre fermentation produces gas (majorly carbon dioxide, hydrogen, and methane) and SCFAs. In fact, Inulin tends to produce more hydrogen and total gas compared to other soluble fibres (wheat dextrin, psyllium and PHGG)[1,2]. Inulin fermentation majorly tends to produce butyrate.

Due to the presence of Fructans and other inherent components the gas produced by the fermentation of inulin fibres may cause undesirable cramps, bloating, and flatus [3].

FOS/GOS - weak laxative effect / Inulin – mild laxative effect

Soluble Only Fibre

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PSYLLIUM – THE HUSK OF PLANTAGO OVATA PLANT SEEDS

Psyllium is a soluble fibre only. It consists of highly branched and gel-forming arabinoxylan, a polymer rich in arabinose and xylose which has limited digestibility in humans. [4]. The gel forming properties can pose an inconvenience for preparation due to the more limited time to consume drinks. Several members of the intestinal microbiota can at least partially utilize these oligosaccharides and their constituent sugars as an energy source as shown in some studies [5-8].

In a recent randomised, placebo-controlled, double-blinded study, psyllium supplement had a small, but notable effect on the microbial composition of healthy adults (increasing Veillonella and decreasing Subdoligranulum), in constipated subjects there were greater effects on the microbial composition (increased Lachnospira, Faecalibacterium, Phascolarctobacterium, Veillonella and Sutterella and decreased uncultured Coriobacteria and Christensenella) and alterations in the levels of acetate and propionate. Encouragement in the production of acetate and propionate by Psyllium is also confirmed in-vitro [4].

Psyllium is capable of retaining water in the small intestine, and thereby, increasing water flow into the proximal/ascending colon. The resulting increase in the fluidity of colonic content may explain the success of psyllium husk in managing constipation [7,8,9]. In addition to the relief of symptoms through softening of stool and increasing stool frequency, the increase of free water alters the environmental conditions of the colon [3].

In addition to managing constipation, the high water-holding capacity of non-fermented psyllium is effective for attenuating loose/liquid diarrheal stools [9-11] and reducing faecal incontinence episodes [12].

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Soluble Only Fibre

RESISTANT STARCH – EXTRACT AND MULTIPLE PROCESSING TECHNIQUES

Resistant starch is soluble only, fermentable dietary fibre that generally favours butyrate production.

Various techniques such as heat, enzymatic, heat with enzyme and chemical treatments are being practiced for production of resistant starch.

The granular type RS2 which are extracted and available in pure form are found to increase butyrate production. Is known to influence the abundance of BIFIDOBACTERIUM and BACTEROIDES IN IN-VITRO BATCH FERMENTATION WITH HUMAN FAECES) [13] AND IN ANIMAL MODELS [14,15]. It is unclear whether we can assume that all types of RS offer the same health benefits. More research is needed in this area.

In a study confirming previous rodent work, resistant starch increased intestinal nutrient flow and caused selective proliferation of Bifidobacterium [21] and Bacillus species [22] in pigs. In rats, RS2 selectively increases propionate concentrations and Ruminococcus bromii abundance, whereas RS4 feeding increases butyrate and propionate concentrations and is associated with proliferation of Lactobacillus gasseri and Parabacteroides distasonis [23]. A human study has recently confirmed that RS4, in the form of butyrylated starch, leads to higher butyrate delivery to the colon than RS2, which, overall, increases both butyrate and propionate concentrations in the colon compared to an equivalent dose of RS2 [24].

In this study resistant starch demonstrated its mild laxative properties, thus only minor effect on bowel habit.

RPS (MSPREBIOTIC®) supplementation in a clinical trial in elderly (ELD- ≥ 70 years age) and mid-age adult (MID, 30–50 years age) There was a significant increase in Bifidobacterium in both ELD and MID compared to placebo (P = 0.047 and 0.006, respectively). There was a small, but reported increase in the stool butyrate levels in the ELD on MSPREBIOTIC® versus placebo [25]

.

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Soluble Only Fibre

GUAR GUM - EXTRACTED GALACTO-MANNAN POLYSACCHARIDE

Guar gum is a soluble only fibre, food additive. Partially hydrolysed Guar Gum (PHGG) is non-viscous (though original non-hydrolysed version is viscous) and highly fermentable. Guar gum is a galacto-mannan polysaccharide extracted from beans for its thickening and stabilising properties. The guar seeds are mechanically dehusked, hydrated, milled, and screened according to application.

In a recent clinical trial [28], consumption of PHGG was determined to influence microbial diversity, along with increasing abundances of metabolites including butyrate, acetate and various amino acids. PHGG intake was associated with a bloom in Ruminococcus, Fusicatenibacter, Faecalibacterium and Bacteroides and a reduction in Roseburia, Lachnospiracea and Blautia.

The majority of effects disappeared after stopping the prebiotic and most effects tended to be more pronounced in male participants. The major SCFA produced is butyrate as confirmed by clinical studies [26-28].

Effects on stool frequency is inconsistent with one study supporting the PHGG to improve stool form and frequency [28] while others contradict in that PGHH only showed improvement in stool form but no effect on stool frequency [27]. Interestingly the study that reports improvement with PHGG [28], while there was an increase in stool frequency and consistency (Bristol Stool Score value) in male participants, female participants did not experience such effects upon PHGG supplementation.

At 5g dose in healthy volunteers reported to cause symptoms (bloating, abdominal distension, flatulence) compared to standard meal and psyllium. Symptoms were correlated to gas production.

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Soluble Only Fibre

WHEAT DEXTRIN – CHEMICALLY ALTERED WHEAT STARCH

Wheat Dextrin is a soluble fibre only.

Higher dosage (60 and 80g/day) is reported to cause flatulence and bloating [3,29]. Digestive tolerance threshold is 45g/d [29].

An increase in the abundance of Bifidobacterium, Lactobacilli, Roseburia and Clostridium cluster XIVa upon addition of wheat dextrin has been reported [30]. Induce rapid decrease in pH due to lactic acid SCFA production, leading to possible osmotic effects and laxativity [29].

At 5% Nitriose, added to control diet of rats stimulated acetate and butyrate production in caecum [31], and reported to be a very good substrate for caecal fermentation indicated by increase in caecal weight. Wheat dextrin produces higher propionate than inulin [3].

Two fibres (soluble/fermented wheat dextrin and finely ground insoluble wheat bran) have actually been shown to decrease stool water content, resulting in a constipating effect [32-34].

û Not suitable for Coeliac or wheat allergy

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Soluble Only Fibre

WHEAT BRAN – OUTER SHELL OF WHEAT GRAIN

Wheat bran is a by-product of wheat grain milling and grinding. The physiological effects of wheat bran can be split into the following: nutritional effects from its constituent nutrients; mechanical effects in the gastrointestinal tract due to its fibre content; and antioxidant effects arising from its phytochemical constituents. Wheat bran has higher antioxidant activity than other milled fractions, and contains various components such as phytic acid, polyphenols (including lignans and phenolic acids), vitamins, and minerals.

Wheat bran, however, has the disadvantage of containing 3% phytate* which has been considered as an anti-nutrient in humans because of its negative effect on the bioavailability of iron, magnesium, zinc and calcium. An average 2–3 tablespoon serving of commercial wheat bran is estimated to contain 200–300 mg of phytic acid.

When it comes to IBS, wheat bran may not be universally beneficial in this condition due to its high FODMAP content.

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Wheat barn can elicit a good laxative effect however it can increase gas production, feelings of abdominal distension and incomplete emptying of the rectum

û Not suitable for Coeliac or wheat allergy

Insoluble Fibre

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METHYL CELLULOSE – SEMI SYNTHETIC HYDROXYPROPYL METHYLCELLULOSE

Methyl Cellulose is a non/poorly fermentable dietary fibre [35].

Hydroxypropyl methylcellulose, semisynthetic cellulose derivative with hydroxypropyl and methyl side chains was shown to influence microbiota in rats [36]. The data however needs further confirmation in humans.

Hydroxypropyl methylcellulose is not absorbed by the host [37], however, it can modulate the intestinal nutrient environment by selectively increasing the excretion of faecal bile acids and fats [38] and increasing faecal water content [35], and thus could have downstream effects on the intestinal microbiota.

Insoluble Fibre

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SUMMARY TABLE

Fibre type Gut transit time Laxation Effect on Microbiota

SCFA Gas Production

Negative effects

happiGut™ -

Virgin Sugarcane Fibre

Increases gastric emptying rates

Good laxative effect

True insoluble fibre

Promotes non-selective microbial growth & diversity

Uniform rate of fermentation along the entire colon

Broad SCFA type production

Low None

FOS/GOS/

(Soluble/highly fermentable short-chain oligosaccharide)

Does not speed up transit time/delays gastric emptying

Weak laxative effect

Promote selective growth of specific microbiota e.g., Bifidobacteria. May reduce other specific butyrate-producing bacteria

Rapidly fermented in terminal ileum and proximal colon to produce SCFAs, major SCFA is butyrate.

High

Rapid fermentation may contribute to gas, flatulence and GI symptoms.

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Inulin (Soluble/highly fermentable long-chain oligosaccharide)

may delay gastric emptying at higher doses

Mild laxative effect

Increases overall bacterial diversity but could be selective for Bifidobacteria

Rapidly fermented in proximal colon to produce SCFAs., majorly butyrate

high Rapid fermentation may contribute to gas, flatulence and GI symptoms

Fibre type Gut transit time Laxation Effect on Microbiota

SCFA Gas Production

Negative effects

RS, Pectin, Guar Gum

(soluble highly fermentable)

Does not speed up transit time.

Can slow absorption from small intestine.

Mild laxative effect

Increases overall bacterial diversity but could be selective for Bifidobacteria.

RS promotes Bifidobacteria and Ruminococcus

Rapidly fermented in proximal colon to produce SCFAs. RS majorly produces butyrate.

High to moderate

Rapid fermentation may contribute to gas, flatulence and GI symptoms

Psyllium (intermediate soluble fermentable)

Does speed-up transit time

Good laxative effect

increases overall bacterial diversity but little evidence for selective growth

Moderately fermented to produce SCFAs along the length of the colon.

Moderate to high

Side-effects of gas, flatulence, abdominal distention and bloating reported for healthy volunteers.

Wheat bran (insoluble slowly fermentable)

Does speed-up transit time

Good laxative effect

increases overall bacterial diversity but little evidence

slowly fermented to produce SCFA along the

Moderate to high

excessive gas / wind and bloating. May not suit IBS

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for selective growth

length of the colon

Cellulose, methylcellulose (insoluble, non-fermentable)

Does speed-up transit time

Good laxative effect

No evidence for direct selective growth

Poorly fermented

low Less gas / wind forming properties

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