Upload
vuongliem
View
231
Download
6
Embed Size (px)
Citation preview
SAPPHiRE BIOSCIENCE
~~
() GeneTex ~
Dengue Virus Antibodies
The Dengue virus (DEN) is of the genus Flavivirus and possesses a
posit ive-strand RNA genome of approximately 10.6 kb that conta ins a ~
single open-reading frame encoding for a polyprote in, which can be I ' further cleaved by cellular and viral proteases into three structural
proteins (capsid prote in C, membrane protein M, envelope protein
E) , and seven non-structural proteins (NS1 , NS2a , NS2b , NS3 ,
NS4a , NS4b, NS5). The re are four serotypes (DEN1 -4) , though
there is certa inly genotypic variability in individual viral genomes
within each serotype . This variability is thought to have minimal
clinical significance, as human infection from any of the four serotypes
results in long -term immunity to that serotype . However, cross-i mmun ity between serotypes is of much shorter
duration , meaning that many people are victims of sequential infection by different serotypes . This is clinically
very important , as sequential infection appears to be a risk factor for more serious disease manifestations.
Dengue Virus Protein Schematic Diagram
II. ___ .11 Capsid Prm Envelop NS1 NS28 NS3 NS4A NS48
(GTX103343) (GTX 128093) (GTX629116) (GTX630556) (GTX 124246) (GTX629477) (GTX 124249) (GTX124250)
kDa
NS5 (GTX629447)
kDa 72-55-43-
34-26 -
17-
kDa 72- A B 55-43-34-
26- -17-
kDa A B
70 -55-40-
35-25-
WB analysis of virus-infected BHK21 cells.
kDa i30- A B
100 -
70-
55 -
40 -
35-
-kDa A B 95-72 -55 -43 -
34-
26 -
WB analysis with GeneTex dengue virus antibodies on virus-infected BHK21 cells.
NS1 (Dengue virus) antibody [GT1061] (GTX630557) ICC/IF with NSl (Dengue virus) antibody on BHK-21 cells infected with Dengue 2 virus.
kDa 170- A B
130-
95-
72-
55--
kDa 130- ABC 72 -55-43-34-26-
- +-17-
10-
Mock
i30- A B
95-72 -
55-
43 -
34-
26-
130 -100-70-
55-
40-
35-
25_
A B
Dengue 2 virus infected
NS3 (Dengue virus) antibody [GT2811] (GTX629477) ICC/IF with NS3 (Dengue virus) antibody on BHK-21 cells infected with Dengue 2 virus.
www.genetex.com
•
• •
• • • • • •
I
I
I , , ,
I
Mock
20~m
20~m
NS4B (Dengue virus) antibody (GTX1 03349) ICC/IF with NS4B (Dengue virus) antibody on BHK-21 cells infected with Dengue 2 virus.
N
'" o <Xl N ,....,
~ \!l
I
I I
I
I
Mock
20~m
20~m
PrM (Dengue virus) antibody (GTX128092) ICC/IF with PrM (Dengue virus) antibody on BHK-21 cells infected with Dengue 2 virus.
C!malH!l. 1;Ojf'!DIiI1
., GTX29202 Dengue virus 1, 2, 3 &4 antibody [D1·11(3)]
GTX41428 Dengue Virus Type 2 antibody [NYRDeng2]
GTX43295 Dengue Types 1,2,3,4 antibody [BDI419]
GTX41437 Dengue Virus antibody [5161]
GTX41430 Dengue Virus Type 2 antibody [3H5.1]
GTX43296 Dengue Type 2, envelope antibody [B932M]
~ GTX124247 Capsid Protein (Dengue virus) antibody
GTX103343 Capsid Protein (Dengue virus) antibody
GTX128092 PrM (Dengue virus) antibody
~ GTX128093 PrM (Dengue virus) antibody
GTX127277 Envelope protein (Dengue virus) antibody
GTX629116 Envelope protein (Dengue virus) antibody [GT214]
GTX629117 Envelope protein (Dengue virus) antibody [GT643]
GTX630557 NS1 (Dengue virus) antibody [GT1061]
GTX630556 NS1 (Dengue virus) antibody [GT4212]
~ GTX103346 NS1 (Dengue virus) antibody
GTX124280 NS1 (Dengue virus) antibody
~ GTX124246 NS2B (Dengue virus) antibody
~ GTX124252 NS3 (Dengue virus) antibody
GTX629477 NS3 (Dengue virus) antibody [GT2811]
~ GTX124249 NS4A (Dengue virus) antibody
GTX103349 NS4B (Dengue virus) antibody
~ GTX124250 NS4B (Dengue virus) antibody
~ GTX103350 NS5 (Dengue virus) antibody
GTX124253 NS5 (Dengue virus) antibody
GTX629446 NS5 (Dengue Virus 2) antibody [GT353]
GTX629447 NS5 (Dengue virus 2) antibody [GT361]
Mock Dengue 2 virus infected
Envelope protein (Dengue virus) antibody [GT643] (GTX629117) ICC/IF with Envelope protein (Dengue virus) antibody on BHK-21 cells infected with Dengue 2 virus.
Mock Dengue 3 virus infected
Capsid Protein (Dengue virus) antibody (GTX124247) ICC/IF with Capsid Protein (Dengue virus) antibody on BHK-21 cells infected with Dengue 3 virus.
.. ~ • ' .. • DEN Ms mAb Dot, ELISA, ICCIIF, WB
DEN2 Ms mAb ELISA, WB
DEN Ms mAb Dot, ELISA, ICCIIF
DEN Ms mAb ELISA
DEN2 Ms mAb ICCIIF
DEN2 Ms mAb ICC/IF
Capsid (DEN) Rb pAb ICCIIF, WB
Capsid (DEN) Rb pAb ICCIIF, WB
PrM (DEN) Rb pAb ICC/IF , WB
PrM (DEN) Rb pAb ICCIIF, WB
Envelope (DEN) Rb pAb ICCIIF, WB
Envelope (DEN) Ms mAb ICC/IF, WB
Envelope (DEN) Ms mAb ICCIIF, WB
NS1 (DEN) Ms mAb ICCIIF, WB
NS1 (DEN) Ms mAb ICCIIF, WB
NS1 (DEN2) Rb pAb ELISA, ICCIIF, WB
NS1 (DEN) Rb pAb ICCIIF, WB
NS2B (DEN) Rb pAb ICCIIF, WB
NS3 (DEN) Rb pAb ICC/IF , IHC·P, WB
NS3 (DEN) Ms mAb ICCIIF, WB
NS4A (DEN) Rb pAb ICC/IF , WB
NS4B (DEN) Rb pAb ICC/IF, WB
NS4B (DEN) Rb pAb ICCIIF, WB
NS5 (DEN) Rb pAb ICC/IF , WB
NS5 (DEN) Rb pAb ICC/IF , WB
NS5 (DEN) Ms mAb ICCIIF, WB
NS5 (DEN) Ms mAb ICCIIF, WB
Distributed by: SAPPHIRE In Australia : Sapphire Bioscience Ply. Ltd. Tel: +61 296982022 Fax: +61 296981022 Email: [email protected] BIOSCIENCE