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Demystifying the Laboratory
2
Outline• Role of a regulatory laboratory• Where do samples come from?• What do we test?• Laboratory programs• Rapid Response Team program• RRT investigations• Investigations involving environmental samples
3
Food Laboratory Mission:• To provide expert state of the art
analytical testing in support of food safety and security programs, and consumer and agricultural interests in New York State.
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Division of Food Laboratory
Albany, NY
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Food Laboratory sections:• Food and Dairy Microbiology: analyze over 15,000
food samples/yr. ~ 2,500 of these for foodborne pathogens and the remainder for other indicators
• Food and Dairy Chemistry: analyze over 5,000 food samples/yr. ~ 1,500 for chemical hazards
• Pesticide residues: analyze over 2,500 produce samples/yr. for 165 different pesticide residues and pharmaceutical products
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What do we test? • Primarily testing for specific health hazards and/or
accuracy of labeling
• Focus on high risk, ready-to-eat food, feed, andenvironmental samples
• Collaborate and coordinate work planning with other allied public health agencies (FDA, USDA, EPA, NYSDOH, etc.)
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Division of Food Laboratory • PulseNet Ag laboratory
• Genome Trakr laboratory
• FDA certified for milk testing laboratory
• Member of Food Emergency Response Network (FERN)
• Participant of the Pesticide Data Program (USDA)
Division of Food Laboratory
2008 Accredited to• ISO/IEC 17025 Standard
• AOAC ALACC Criteria: Guidelines for Laboratories Performing Microbiological and Chemical Analysis of Food, Dietary Supplements, and Pharmaceuticals
https://www.a2la.org/scopepdf/2749-01.pdfhttps://www.a2la.org/scopepdf/2749-02.pdf
Division of Food Laboratory
Scope of Accreditation - Chemical• Ash in Feed• Determination of Crude Fiber in Animal Feed• Determination of Cyclamate by UPLC/MS• Determination of Fat, Fiber, Protein and Moisture in Animal Feed by NIR• Determination of Lasalocid in Feed by HPLC/MS/MS• Determination of Minerals in Animal Feed• Determination of Monensin in Feed by HPLC/MS/MS• Determination of Nitrogen in Fertilizer• Determination of Patulin by HPLC/MSMS• Determination of Potash in Fertilizer• Determination of Sulfites• Metal Analysis by Closed Bessel Microwave Digestion an ICP/MS• Multiple Residue Screening of Pesticide Data Program (PDP) Samples• Percent Moisture in Feed and Pet Food• Toxic Metal Analysis in Foods by Closed Vessel Microwave Digestion and ICP/MS
Division of Food Laboratory
Scope of Accreditation - Biological
• Campylobacter jejuni/lari /coli species on BAX
• Campylobacter species USDA Method
• Escherichia coli O157:H7 on ABI 7500 Fast
• Escherichia coli O157:H7 Modified BAM Method
• Detection of Shiga-toxin producing E. coli (STEC) by rtPCR on 7500 ABI Fast
• Listeria monocytogenes on ABI 7500 Fast
• Listeria monocytogenes BAM Method
• Salmonella species on ABI 7500 Fast
• Salmonella species BAM Method
• Phosphatase Testing using the Advanced Instruments Fluorophos
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Where do the samples come from?
• Within Ag and Markets (surveillance)• Division of Food Inspection • Division of Milk Control/Dairy Services• Division of Plant Industry
Collected by field staff from farms, food processing plants, food/feed/fertilizer manufacturers, distribution centers, farmers markets, and retail stores.
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Where do the samples come from?
• Other states • NE Region
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Where do the samples come from?
• Federal agencies• AMS Pesticide Data Program • Food Emergency Response Network (FERN)• Feed Safety Program
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Where do the samples come from?
• Develop and maintain multi-jurisdictional RRTs that operate under ICS/NIMS and Unified Command to support integrated all-hazards prevention, response and recovery efforts for food/feed. Unify and coordinate federal/state/local food/feed emergency response efforts, including:• Strengthening the link among epidemiology, lab and environmental health components.
• Foodborne illness/outbreak investigations, removing tainted food from commerce, root cause investigations.
• Supporting components: training, data sharing, data analysis and communications.
• Capture/develop and support adoption of best practices (mentorship)
• Ensure alignment with national priorities (FSMA, IFSS, PFP)
Rapid Response Teams
“The RRT Five-Legged Stool”
Food
Program Feed
Program
FDA
District
LaboratoryEpidemiology
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Outbreaks & Recall Investigations• Salmonella associated with deli meats/spices (2010)
• Shiga-toxin producing E. coli O145 in lettuce (2010)
• Listeria monocytogenes in potato salad (2011)
• Salmonella in chicken livers (2011)
• E. coli O157:H7 associated with produce (2013)
• Listeria monocytogenes in imported seafood (2012-13)
• Salmonella in pet foods/treats (2013)
• Salmonella in chia seeds (2014)
• Listeria monocytogenes in soft cheese (2016-17)
• Listeria monocytogenes in deli markets (ongoing)
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RRT Investigations• From December- October 2017
• Six events investigating presence of Salmonella Enteriditis and Listeria monocytogenes• Four joint investigation with FDA (NYSDAM-product samples and FDA-environmental)• Most samples tested in the laboratory were negative except for one…..
• February 2017• Listeria monocytogenes in raw milk cheese
• Joint investigation with FDA (NYSDAM-product samples;FDA-environmental and product samples)• 6 samples were taken. Three samples positive.
Listeria monocytogenes in Raw Milk Soft Cheese
• Firm issues nationwide recall of product 3/7/17
• PFGE performed by NYS Ag and Markets Food Laboratory on cheese isolates patterns matched to clinical isolates 3/8/17
• WGS performed by NYS Ag and Markets Food Laboratory on cheese isolates and FDA matches sequence to ≤3 SNPs difference to those of the clinical isolates 3/13/17
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Investigations involving Environmental Sampling• From January - October 2017
• Seven environmental sampling events investigating presence of Listeria monocytogenes in deli markets
• Swabs are collected from different zones
• Five events resulted in positive results (environmental or food)
• Once positive samples are identified, Food Safety inspectors return to the establishment and request compliance of the “Listeria protocol”.
Listeria monocytogenes –environmental samples
PFGE-ApaI100
90
80
PFGE-ApaI PFGE-AscI
FCF__0968336-S002-001
FCF__0968336-S022-001
17B01532M-1
CO___HUM-2016023857
USDA_210261727
USDA_210262277
FCF__0968336-S005-001
FCF__0968336-S013-001
FCF__0968336-S014-001
FCF__0968336-S023-001
FCF__0968336-S027-001
FCF__0968336-S029-001
FCF__0968336-S030-001
FCF__0968336-S031-001
FCF__0968336-S035-001
FCF__0968336-S046-001
Serving toolHuman (2016)
Listeria monocytogenes –environmental samples
PFGE-AscI
10
0
90
80
70
PFGE-AscI
20
.00
40
.00
10
0.0
0
15
0.0
0
20
0.0
0
25
0.0
0
30
0.0
0
35
0.0
0
40
0.0
0
50
0.0
0
60
0.0
0
20
00
1.0
0E
4
5.0
0E
4
PFGE-ApaI
20
.00
40
.00
10
0.0
0
15
0.0
0
20
0.0
0
25
0.0
0
30
0.0
0
35
0.0
0
40
0.0
0
50
0.0
0
60
0.0
0
20
00
1.0
0E
4
5.0
0E
4
15B07268-10
15B07268-6
17B06335L-5
17B06335L-6
17B06335P-7
17B06335P-8
17B06335T-5
17B07453-9
17B10701-D1
17B10701-D10
17B10701-F9
17B10701-H10
17B10701-H8
17B10701-K1
17B10701-K8
17B10701-M4
17B10701-M7
17B10701-N7
17B10701-O1
LM-1.2W4-103116
LM-2 WK1 080817
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
NYAG
USA
USA
USA
USA
USA
USA
USA
USA
USA
USA
USA
USA
USA
USA
USA
USA
USA
USA
USA
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Bronx
Deli meat
Deli meat
Sponge
Sponge
Sponge
Sponge
Sponge
Deli Meat
Sponge
Sponge
Sponge
Sponge
Sponge
Sponge
Sponge
Sponge
Sponge
Sponge
Sponge
Food
Food
Environmental
Environmental
Environmental
Environmental
Environmental
Food
Environmental
Environmental
Environmental
Environmental
Environmental
Environmental
Environmental
Environmental
Environmental
Environmental
Environmental
mortadella w/ pistachio
mortadella w/ pistachio
Ware wash sink
Ware wash sink
Deli case #1
Deli case #1
Deli case #4
mortadella w/pistachio
back cooler #1 top
back cooler #1 top
back cooler #3 top
ware wash sink
ware wash sink
slicer machine #3 (for me.
slicer machine #3 (for me.
deli slicer #5 (for cheese)
deli slicer #5 (for cheese)
deli case #7 (by exit)
cutting board #4 (front, w.
2015-07-15
2015-07-15
2017-06-06
2017-06-06
2017-06-06
2017-06-06
2017-06-06
2017-06-27
2017-09-26
2017-09-26
2017-09-26
2017-09-26
2017-09-26
2017-09-26
2017-09-26
2017-09-26
2017-09-26
2017-09-26
2017-09-26
2015-07-21
2015-07-21
2017-06-21
2017-06-21
2017-06-21
2017-06-21
2017-06-21
2017-07-06
2017-10-05
2017-10-05
2017-10-05
2017-10-05
2017-10-05
2017-10-05
2017-10-05
2017-10-05
2017-10-05
2017-10-05
2017-10-05
2015-07-23
2017-06-23
2017-06-23
2017-06-23
2017-07-11
2017-10-12
2017-10-12
2017-10-12
2017-10-12
2017-10-12
2017-10-12
2017-10-12
1
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GX6A16.01.
GX6A16.01.
GX6A16.01.
GX6A16.01.
GX6A16.01.
GX6A16.01.
GX6A16.01.
GX6A16.01.
GX6A16.01.
GX6A16.01.
GX6A16.01.
GX6A16.01.
GX6A12.0186
GX6A12.0186
GX6A12.0186
GX6A12.0186
GX6A12.0186
GX6A12.0186
GX6A12.0186
GX6A12.0186
GX6A12.0186
GX6A12.0186
GX6A12.0186
GX6A12.0186
7/15/17
6/6/17
6/27/17
9/26/17
Isolation Date
Acknowledgements
Thank you!
Maria Ishida, PhDDirector of Food Laboratory
NYS Department of Agriculture and MarketsEmail: [email protected]