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DBS Collection Kit- Collection, Storage and Transportation of Dried Blood Spot Samples
AdvantagesSimpler, relatively painless sampling procedure: •
Ideal for Home sampling: No trained Phlebotomist required •and samples can be collected by the patients’ themselves.
No need for maintaining a cold chain for transportation and •storage of the samples: Most analytes in DBS are stable at room temperature for at least a week.
Reduced biohazard: DBS sampling poses reduced biohazard •as many viruses known to be present in serum or plasma lose infectivity as a consequence of disruption of their envelope on drying.
Significant Cost-reduction: Cost reduction for tech time, •sample collection, storage and transportation by DBS as compared to wet samples can be several folds.
Applications
DBS testing is widely used within the fields of Neonatal Metabolic Screening, Diagnostics, Forensics, Health and Wellness, Clinical and Preclinical Pharmacokinetics and Toxicokinetics studies and Therapeutic Drug Monitoring. It is particular useful for field research. DBS is routinely used to measure metabolites (lipids and amino acids), nucleic acid (DNA and mRNA) and proteins (hemoglobin, cytokines etc.) both qualitatively and quantitatively.
www.lipidx.com/dbs-application/ lists a selected number of analytes that can be measured using DBS kits.
Customized ServiceTo expand your options for data collected on a card, LipiDx can customize DBS kits so that it is more suited for your specific application. We can customize DBS kits to include your Institution specific information such as address and logo on printed boxes and envelopes. Instructions for sample collection and shipping procedures can be customized to suit your specific purpose. If required, we can include documents like Informed Consent Form, Case Report Form, Lab Request Form or Patient Release Form in the kit suiting your purpose. Any specific barcode preferences (sequential and non-sequential) can be included and data sheets can be customized for optical character recognition (OCR).
GeneralLipiDx DBS collection kit contains all components necessary for cost-effective collection, storage and transportation of dried blood spot (DBS) samples. It can be conveniently used by both patients and practitioners.
Over the past several years DBS sampling has emerged as a potent sampling method for both qualitative and quantitative bioanalysis. Blood sample is directly soaked on to collection card, dried and transported for analysis to a laboratory equipped with modern analytical, immunological or genomic detection systems. Several studies show that DBS sampling provides compatible results as with those performed with fresh blood samples obtained by conventional blood draws.
www.lipidx.comDBS Collection ProtocolDrops of whole blood sample are collected by finger, toe or heel prick with the aid of sterile disposable lancet. A typical drop of bloodcontainsabout50µlofwholeblood.Itisnecessarythatthebloodiscollectedensuringitisabsorbedevenlyacrossthefilter paper and the blood spot is symmetric and circular.
After sample collection, the DBS cards are dried, packed and shipped to analytical laboratories through mail, courier or express mail delivery services. DBS samples can be shipped and stored at ambient temperature.
Please read this User Manual prior to blood spot collection. Complete any accompanied forms, if provided.
First check the content of the Kit. It should contain:
1 Instruction Manual1 lancet1 Sample collection Card1 alcohol cleaning pad1 dry swab1 silica pouch1 sheet barcodes1 metal bag1 Envelope for postage
Note: Do not touch the paper flaps on the Sample Collection Card
Instruction Manual
DBS Collection Kit
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Lipi
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Air-dry blood spots for at least 10 minutes before closing Sample Collection Card. Affix barcode A in window A. Insert the sample Card under the edge to protect the two flaps of collection paper.
Put the closed Sample Collection Card with dried blood into the metal bag together with silica pouch and seal. Affix barcode B to the unmarked side of the metal bag.
Two extra barcodes (C & D) have been provided. These can be affixed on the accompanied docu-ments such as Laboratory Request Form, Case Report Forms, or Informed Consent Form, if required.
Put the sealed metal bag and any document that must be completed and signed into the return envelope and seal. Affix a postage stamp and mail to the address.
6 7 8 97
Please read this User Manual prior to blood spot collection. Complete any accompanied forms, if provided.
First check the content of the Kit. It should contain:
1 Instruction Manual1 lancet1 Sample collection Card1 alcohol cleaning pad1 dry swab1 silica pouch1 sheet barcodes1 metal bag1 Envelope for postage
Note: Do not touch the paper flaps on the Sample Collection Card
Instruction Manual
DBS Collection Kit
Copy
right
© 2
014
Lipi
Dx
AS A
ll rig
hts
rese
rved
.
Air-dry blood spots for at least 10 minutes before closing Sample Collection Card. Affix barcode A in window A. Insert the sample Card under the edge to protect the two flaps of collection paper.
Put the closed Sample Collection Card with dried blood into the metal bag together with silica pouch and seal. Affix barcode B to the unmarked side of the metal bag.
Two extra barcodes (C & D) have been provided. These can be affixed on the accompanied docu-ments such as Laboratory Request Form, Case Report Forms, or Informed Consent Form, if required.
Put the sealed metal bag and any document that must be completed and signed into the return envelope and seal. Affix a postage stamp and mail to the address.
6 7 8 9 8
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© 2
013
BioA
ctiv
e Fo
ods A
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Choose a finger tip (ring finger recommended) and clean well with the alcohol cleaning pad. Allow finger tip to dry. To stimulate blood flow from your selected finger, hang and shake the hand for 25 seconds.
Open Sample Collection Card containing two collection paper flaps. Do not touch the flaps with your finger.
Take out the green, single use lancet. Turn the narrow green plastic stopper 1/4 turn counter-clockwise. Pull out the stopper and dispose. The lancet is ready to use.
Place the lancet on the finger tip and push the top of the lancet against finger until you hear a click; the lancet will prick the finger and make a small incision.
Place at least 3 drops of blood onto each of the two paper flaps in the sample collection card. Wipe off any extra blood on finger with dry swab. Ensure that blood has soaked through both flaps.
1 2 3 4 51
Copy
right
© 2
013
BioA
ctiv
e Fo
ods A
S . A
ll rig
hts r
eser
ved.
Choose a finger tip (ring finger recommended) and clean well with the alcohol cleaning pad. Allow finger tip to dry. To stimulate blood flow from your selected finger, hang and shake the hand for 25 seconds.
Open Sample Collection Card containing two collection paper flaps. Do not touch the flaps with your finger.
Take out the green, single use lancet. Turn the narrow green plastic stopper 1/4 turn counter-clockwise. Pull out the stopper and dispose. The lancet is ready to use.
Place the lancet on the finger tip and push the top of the lancet against finger until you hear a click; the lancet will prick the finger and make a small incision.
Place at least 3 drops of blood onto each of the two paper flaps in the sample collection card. Wipe off any extra blood on finger with dry swab. Ensure that blood has soaked through both flaps.
1 2 3 4 52
Copy
right
© 2
013
BioA
ctiv
e Fo
ods A
S . A
ll rig
hts r
eser
ved.
Choose a finger tip (ring finger recommended) and clean well with the alcohol cleaning pad. Allow finger tip to dry. To stimulate blood flow from your selected finger, hang and shake the hand for 25 seconds.
Open Sample Collection Card containing two collection paper flaps. Do not touch the flaps with your finger.
Take out the green, single use lancet. Turn the narrow green plastic stopper 1/4 turn counter-clockwise. Pull out the stopper and dispose. The lancet is ready to use.
Place the lancet on the finger tip and push the top of the lancet against finger until you hear a click; the lancet will prick the finger and make a small incision.
Place at least 3 drops of blood onto each of the two paper flaps in the sample collection card. Wipe off any extra blood on finger with dry swab. Ensure that blood has soaked through both flaps.
1 2 3 4 53
Copy
right
© 2
013
BioA
ctiv
e Fo
ods A
S . A
ll rig
hts r
eser
ved.
Choose a finger tip (ring finger recommended) and clean well with the alcohol cleaning pad. Allow finger tip to dry. To stimulate blood flow from your selected finger, hang and shake the hand for 25 seconds.
Open Sample Collection Card containing two collection paper flaps. Do not touch the flaps with your finger.
Take out the green, single use lancet. Turn the narrow green plastic stopper 1/4 turn counter-clockwise. Pull out the stopper and dispose. The lancet is ready to use.
Place the lancet on the finger tip and push the top of the lancet against finger until you hear a click; the lancet will prick the finger and make a small incision.
Place at least 3 drops of blood onto each of the two paper flaps in the sample collection card. Wipe off any extra blood on finger with dry swab. Ensure that blood has soaked through both flaps.
1 2 3 4 54
Copy
right
© 2
013
BioA
ctiv
e Fo
ods A
S . A
ll rig
hts r
eser
ved.
Choose a finger tip (ring finger recommended) and clean well with the alcohol cleaning pad. Allow finger tip to dry. To stimulate blood flow from your selected finger, hang and shake the hand for 25 seconds.
Open Sample Collection Card containing two collection paper flaps. Do not touch the flaps with your finger.
Take out the green, single use lancet. Turn the narrow green plastic stopper 1/4 turn counter-clockwise. Pull out the stopper and dispose. The lancet is ready to use.
Place the lancet on the finger tip and push the top of the lancet against finger until you hear a click; the lancet will prick the finger and make a small incision.
Place at least 3 drops of blood onto each of the two paper flaps in the sample collection card. Wipe off any extra blood on finger with dry swab. Ensure that blood has soaked through both flaps.
1 2 3 4 5 5
Please read this User Manual prior to blood spot collection. Complete any accompanied forms, if provided.
First check the content of the Kit. It should contain:
1 Instruction Manual1 lancet1 Sample collection Card1 alcohol cleaning pad1 dry swab1 silica pouch1 sheet barcodes1 metal bag1 Envelope for postage
Note: Do not touch the paper flaps on the Sample Collection Card
Instruction Manual
DBS Collection Kit
Copy
right
© 2
014
Lipi
Dx
AS A
ll rig
hts
rese
rved
.
Air-dry blood spots for at least 10 minutes before closing Sample Collection Card. Affix barcode A in window A. Insert the sample Card under the edge to protect the two flaps of collection paper.
Put the closed Sample Collection Card with dried blood into the metal bag together with silica pouch and seal. Affix barcode B to the unmarked side of the metal bag.
Two extra barcodes (C & D) have been provided. These can be affixed on the accompanied docu-ments such as Laboratory Request Form, Case Report Forms, or Informed Consent Form, if required.
Put the sealed metal bag and any document that must be completed and signed into the return envelope and seal. Affix a postage stamp and mail to the address.
6 7 8 96
Choose a finger tip (ring finger recommended) and clean well with the alcohol cleaning pad. Allow finger tip to dry.
Place the lancet on the finger tip and push the top of the lancet against finger until you hear a click.
Put the closed Sample Collection Card with dried blood into the metal bag together with silica pouch and seal.
Open Sample Collection Card containing two collection paper flaps.
Placeatleast3dropsofblood onto each of the two paper flaps in the sample collection card.
Take out the green, single use lancet. Turn the narrow green plasticstopper1/4turncounterclockwise. Pull out the stopper and dispose.
Air-dry blood spots before closing Sample Collection Card.
Put the sealed metal bag and any document that must be completed and signed into the return envelope and seal.
*More information is provided in the DBS Collection Kit.
Contact Information
International CustomersLipiDx AS
LilleBorgenVei1ANO–0370Oslo,Norway
E-mail: [email protected]:+4799575325
US CustomersLipiDx Healthcare LLC
10FordhamCourtBEARDE19701USA
E-mail: [email protected]:+1(302)8320502
