DAFTAR PUSTAKA - Diponegoro University | Institutional ...eprints.undip.ac.id/43997/9/BAB_VIII.pdf · Ketaren S. Pengantar teknologi minyak dan lemak pangan ... Jusup I, Johan A,

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LAMPIRAN 3. CARA PEMELIHARAAN, PEMBERIAN MINYAK, PEMBERIAN UBI UNGU, DEKAPITASI, DAN PENGAMBILAN SAMPEL

a) Cara pemeliharaan

Tikus wistar berumur 12 minggu dengan berat badan 150-220 gram,

dipelihara pada kandang individual selama 35 hari. Seluruh tikus diberi pakan P-

594 dan air matang ad libitum selama penelitian. Alas kandang terbuat dari serutan

kayu yang diganti secara teratur.

b) Cara memegang tikus dan pemberian minyak goreng pemanasan berulang

Pengambilan tikus dari kandang dilakukan dengan memegang ekornya lalu

tikus diletakkan diatas kawat kasa, selanjutnya bagian punggung tikus dipegang

dengan telapak tangan dengan jari-jari memegang bagian leher sehingga kepala

tikus dalam posisi terangkat ke atas untuk pemberian minyak per oral sonde

sebanyak 3ml.

c) Cara pemberian ubi ungu kukus

Ubi ungu yang telah dikukus dipotong dadu kemudian ditimbang. Masing-

masing tikus kelompok K3 dan P1 mendapat 30 gram ubi ungu diletakkan pada

tempat makanan.

d) Cara dekapitasi dan pengambilan darah

1. Teknik anestesi yang digunakan adalah dengan menggunakan eter. Tikus

wistar dimasukkan ke dalam wadah yang berisi kapas yang sudah dibasahi

dengan eter, kemudian ditutup rapat.

2 Setelah dianestesi, tikus wistar diterminasi dengan dekapitasi leher, dengan

cara meletakkan tikus di atas permukaan rata. Sebuah pinset diletakkan

diatas kuduk tikus. Sambil menekan pinset, ekor tikus ditarik kuat dan

pinset diarahkan ke atas kepala tikus.

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3. Tikus kemudian diletakkan pada posisi terlentang dan seluruh permukaan

abdomen disemprot dengan alkohol 70%. Kemudian dilakukan insisi

vertikal pada regio abdomen menggunakan scalpel. Setelah itu tulang iga

dipotong sehingga dapat terlihat jantung wistar. Darah langsung diambil

dari ventrikel jantung dan aorta abdominalis dengan spuit dan ditampung di

dalam tabung reaksi.

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LAMPIRAN 4. KOMPOSISI DIET STANDAR P-594

Kandungan Persentase

Kadar air Maksimal 13,0%

Protein 17,5-19,5%

Lemak Minimal 3,0%

Serat Maksimal 8,0%

Abu Maksimal 7,0%

Kalsium Minimal 0,9%

Fosfor Minimal 0,6%

Bahan-bahan yang dipakai, antara lain:

1. Jagung

2. Dedak

3. Tepung ikan

4. Bungkil

5. Tepung daging

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LAMPIRAN 5. HASIL UJI LABORATORIUM

No Kelompok Kode Tikus Kadar TG

(mmol/L)

Kadar HDL

(mmol/L)

1 K1 A 49.0 47.0

2 K1 B 98.0 36.0

3 K1 C 59.0 45.0

4 K1 D 98.0 43.0

5 K1 E 75.0 64.0

6 K2 A 109.0 54.0

7 K2 B 72.0 48.0

8 K2 C 140.0 32.0

9 K2 D 119.0 40.0

10 K2 E 73.0 45.0

11 K3 A 116.0 36.0

12 K3 B 135.0 45.0

13 K3 C 152.0 37.0

14 K3 D 150.0 76.0

15 K3 E 137.0 56.0

16 P1 A 132.0 38.0

17 P1 B 99.0 33.0

18 P1 C 165.0 39.0

19 P1 D 99.0 65.0

20 P1 E 102.0 58.0

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LAMPIRAN 7. HASIL UJI STATISTIK

1. Jumlah sampel tiap kelompok

Case Processing Summary

Kelomp

ok

Cases

Valid Missing Total

N Percent N Percent N Percent

Kadar TG K1 5 100.0% 0 .0% 5 100.0%

K2 5 100.0% 0 .0% 5 100.0%

K3 5 100.0% 0 .0% 5 100.0%

P 5 100.0% 0 .0% 5 100.0%

Kadar HDL K1 5 100.0% 0 .0% 5 100.0%

K2 5 100.0% 0 .0% 5 100.0%

K3 5 100.0% 0 .0% 5 100.0%

P 5 100.0% 0 .0% 5 100.0%

2. Analisis deskriptif pengaruh pemberian ubi ungu terhadap kadar trigliserida dan

HDL serum

Descriptives

Kelompok Statistic Std. Error

Kadar TG K1 Mean 75.8000 9.96695

95% Confidence Interval for

Mean

Lower Bound 48.1273

Upper Bound 103.472

5% Trimmed Mean 76.0556

Median 75.0000

Variance 496.700

Std. Deviation 22.28681

Minimum 49.00

Maximum 98.00

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Range 49.00

Interquartile Range 44.00

Skewness -.079 .913

Kurtosis -2.521 2.000

K2 Mean 102.602 13.26876


Mean

Lower Bound 65.7600

Upper Bound 139.442


Median 109.002

Variance 880.300


Minimum 72.00

Maximum 140.00

Range 68.00


Skewness .038 .913

Kurtosis -2.072 2.000

K3 Mean 138.002 6.45755


Mean

Lower Bound 120.072

Upper Bound 155.932


Median 137.002

Variance 208.500


Minimum 116.00

Maximum 152.00

Range 36.00


Skewness -.859 .913

Kurtosis .439 2.000

75

P Mean 119.402 12.98692


Mean

Lower Bound 83.3425

Upper Bound 155.462


Median 102.002

Variance 843.300


Minimum 99.00

Maximum 165.00

Range 66.00


Skewness 1.269 .913

Kurtosis .414 2.000

Kadar HDL K1 Mean 47.0000 4.63681


Mean

Lower Bound 34.1262

Upper Bound 59.8738


Median 45.0000

Variance 107.500


Minimum 36.00

Maximum 64.00

Range 28.00


Skewness 1.312 .913

Kurtosis 2.647 2.000

K2 Mean 43.8000 3.72022


Mean

Lower Bound 33.4710

Upper Bound 54.1290


Median 45.0000

76

Variance 69.200


Minimum 32.00

Maximum 54.00

Range 22.00


Skewness -.406 .913

Kurtosis .023 2.000

K3 Mean 50.0000 7.42294


Mean

Lower Bound 29.3906

Upper Bound 70.6094


Median 45.0000

Variance 275.500


Minimum 36.00

Maximum 76.00

Range 40.00


Skewness 1.160 .913

Kurtosis .661 2.000

P Mean 46.6000 6.26578


Mean

Lower Bound 29.2034

Upper Bound 63.9966


Median 39.0000

Variance 196.300


Minimum 33.00

Maximum 65.00

Range 32.00


77

Skewness .624 .913

Kurtosis -2.338 2.000

3. Hasil uji normalitas data pengaruh pemberian ubi ungu terhadap kadar

trigliserida dan HDL serum

Tests of Normality

Kelomp

ok

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

Kadar TG K1 .240 5 .200* .887 5 .342

K2 .241 5 .200* .900 5 .410

K3 .218 5 .200* .910 5 .467

P .325 5 .090 .796 5 .076

Kadar HDL K1 .300 5 .161 .891 5 .363

K2 .157 5 .200* .990 5 .979

K3 .218 5 .200* .882 5 .317

P .306 5 .141 .867 5 .256

a. Lilliefors Significance Correction

*. This is a lower bound of the true significance.

4. Hasil uji homogenitas varians kadar trigliserida dan HDL serum

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig.

Kadar TG Based on Mean 1.604 3 16 .228

Based on Median .480 3 16 .701

Based on Median and with

adjusted df.480 3 9.147 .704

Based on trimmed mean 1.530 3 16 .245

Kadar HDL Based on Mean 1.447 3 16 .266

Based on Median .473 3 16 .705

Based on Median and with

adjusted df.473 3 12.541 .706

Based on trimmed mean 1.351 3 16 .293

78

5. Hasil uji parametrik One-Way ANOVA kadar trigliserida dan HDL serum

ANOVA

Sum of Squares df Mean Square F Sig.

Kadar TG Between Groups 10461.750 3 3487.250 5.743 .007

Within Groups 9715.200 16 607.200

Total 20176.950 19

Kadar HDL Between Groups 96.550 3 32.183 .199 .896

Within Groups 2594.000 16 162.125

Total 2690.550 19

6. Hasil uji Post hoc LSD kadar trigliserida serum

Multiple Comparisons

Kadar TG

LSD

(I)

Kelomp

ok

(J)

Kelomp

ok

Mean Difference

(I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

K1 K2 -26.80000 15.58461 .105 -59.8379 6.2379

K3 -62.20000* 15.58461 .001 -95.2379 -29.1621

P -43.60000* 15.58461 .013 -76.6379 -10.5621

K2 K1 26.80000 15.58461 .105 -6.2379 59.8379

K3 -35.40000* 15.58461 .037 -68.4379 -2.3621

P -16.80000 15.58461 .297 -49.8379 16.2379

K3 K1 62.20000* 15.58461 .001 29.1621 95.2379

K2 35.40000* 15.58461 .037 2.3621 68.4379

P 18.60000 15.58461 .250 -14.4379 51.6379

P K1 43.60000* 15.58461 .013 10.5621 76.6379

K2 16.80000 15.58461 .297 -16.2379 49.8379

K3 -18.60000 15.58461 .250 -51.6379 14.4379

*. The mean difference is significant at the 0.05 level.

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LAMPIRAN 8. DOKUMENTASI

Ubi ungu dibeli dari Pasar Bandungan, Kabupaten Semarang

Hewan coba dikandangkan individual

Ubi dikukus pada suhu 75oC selama 20 menit Ubi dipotong dadu dan ditimbang 30 gram

Proses pemanasam minyak goreng 200oC selama 15 menit

Pemberian minyak 3ml/hari per oral sonde

80

Pemberian ubi ungu kukus ad libitum Sisa ubi ditimbang tiap esok paginya

Pengukuran berat badan akhir Terminasi dan pengambilan darah wistar

Pengepakan sampel darah Pemeriksaan kadar Trigliserida dan HDL menggunakan COBAS INTEGRA

analyzer di Laboratorium CITO Semarang

81

LAMPIRAN 9. BIODATA MAHASISWA

I. IDENTITAS

1. Nama Lengkap : Ivana Yulia Puspowardojo

2. NIM : G2A009078

3. Tempat, Tanggal Lahir : Semarang, 15 Juli 1991

4. Jenis Kelamin : Wanita

5. Alamat Rumah : Jl. Sompok 46 Semarang 50249

6. E-mail : [email protected]

7. Telepon rumah / HP : (024)8314992/+6285725805491

II. PENDIDIKAN

No. Tingkat Nama & Tempat Tahun Lulus

1. Sekolah Dasar SD Kristen 2 YSKI 2003

2. Sekolah Menengah Pertama SMP Kristen YSKI 2006

3 Sekolah Menengah Akhir SMA Kristen YSKI 2009

4. Perguruan Tinggi Fakultas Kedokteran, UNDIP 2009 – sekarang

III. PENGALAMAN ORGANISASI

No. Jabatan Tahun Nama Organisasi

1. Anggota 2010 – 2011 Asian Medical Student Association

2. Anggota 2010 – 2011 Persekutuan Mahasiswa Kristen Kedokteran

3. Anggota 2006 – sekarang Komisi Musik GKI Peterongan

IV. PENGALAMAN KEPANITIAAN

No. Jabatan Tahun Nama Kepanitiaan

1. Sekretaris Februari - Mei

2010

Paskah Bersama PMKK – PRMK

2. Koordinator

Sie Acara

September –

Desember 2010

Natal Bersama PMKK – PRMK, Brayat

Minulyo dan PD Immanuel

3. Anggota September – Bakti Sosial Natal PMKK – PRMK

82

Desember 2010

4. Anggota Juni 2011 Seminar Hidup Lebih Berkualitas Bagi

Thalasemia

5. Sie Acara Agustus –

Oktober

Seminar Cardiorespiratory: Reaching Healthy

Cardiorespiratory System in The Modern Era

6. Anggota Desember 2011 The 6th National Meeting of Indonesian

Network for Bioethics and Medical Humanities

7. Sie

Pengabdian

Masyarakat

Maret 2012 Kemah Bakti Fakultas Kedokteran UNDIP

8. Sie Acara Juli 2013 Pengabdian Masyarakat Bulu Lor

V. PENGALAMAN BEKERJA

No. Jabatan Tahun Nama Instansi

1. Asisten Dosen 2010 – 2012 Biokimia Fakultas Kedokteran UNDIP

VI. PRESTASI

No. Prestasi Bulan, Tahun Nama Kompetisi

1. Juara 1 Desember 2009 Jinggle Malam Puji – Pujian PMK Universitas

Diponegoro

2. Distiction September 2010 The Associated Board of the Royal Schools of

Music, Royal Academy of Music, London

5. Finalis Oktober Hibah Penelitian UP3 Fakultas Kedokteran

UNDIP

7. Juara 3 Oktober –

November 2012

Mahasiswa Berprestasi Jurusan Kedokteran

Umum FK UNDIP

8. Finalis April 2013 Putri Kartini Dharma Wanita Persatuan

UNDIP 2013

83

VII. PENELITIAN

1. Wibawa SR., Puspowardojo IY., Dewi S., Efek Antioksidan dan

Olahraga terhadap Penurunan MDA (Malondialdehid) pada Tikus

dengan Diet Minyak Goreng Pedagang Kaki Lima, 2011.

2. Wibawa SR., Puspowardojo IY., Pramono B., Efek Whole Grain

Cereal terhadap Insiden Penyakit Kardiovaskular, 2011.

3. Ngestiningsih D., Nurhayati D., Puspowardojo IY., Pengaruh

Pemberian Buah Naga Merah, Ubi Ungu, dan Tempe Terhadap Kadar

MDA Darah Mencit yang Diberi Minyak Jelantah, 2012.

4. Puspowardojo IY., Putri AD., Dewi S., Suryajaya C., Potensi Ubi

Ungu sebagai Agen Hipokolesterolemik, Antioksidan dan Penambah

Darah, 2012.

5. Ngestiningsih D., Jusup I., Puspowardojo IY., Hubungan Kadar

MDA Otak dan Kadar TG-HDL Plasma: Studi Pengaruh Antioksida Ubi

Ungu pada Tikus Wistar yang Terpapar Minyak Goreng Berulang, 2013

Documents

DAFTAR PUSTAKA - Diponegoro University | Institutional ...eprints.undip.ac.id/43997/9/BAB_VIII.pdf · Ketaren S. Pengantar teknologi minyak dan lemak pangan ... Jusup I, Johan A,