Centre of Applied Research of Vegetables and Special Crops of Crop Research Institute (CRI) in Praha-Ruzyně

Department of Genetic Resources for Vegetables, Medicinal and Special Plants in Olomouc

Czech Republic

Czech National Collection of Leafy Vegetables

Ivana Doležalová

Plant Genetic Resources in the Czech Republic • The systematic collecting, study and conservation of plant

germplasm is supported by the Ministry of Agriculture • The National Programme on Conservation and Utilization of

Plant Genetic Resources and Agrobiodiversity (2012-2016), Law No. 148/2003

• 12 institutions located in 15 places • Coordinated by the Crop Research Institute in Prague (CRI)

Genetic resources of vegetables (9,245 acc.), medicinal, aromatic and culinary plants (MAPs,

828 acc.) are maintained in Olomouc

Prague

ca 20% of the Czech germplasm Olomouc

Main activities of the Dept. of Genetic Resources for Vegetables, Medicinal and Special Plants in Olomouc

• Gathering (including collection missions) • Documentation and characterization • Regeneration and conservation of GR • Distribution to users • International cooperation (within

ECPGR, AEGIS) • Research activities (e.g. utilization of

neglected crops, MAPs)

Collection of leafy vegetables Family Species EVIGEZ Holding

institution

Asteraceae Lactuca sp. 1408 Dpt. Gen. Res. Veg.

Cichorium endivia 26 Dpt. Gen. Res. Veg. Cichorium intybus 24 Dpt. Gen. Res. Veg.

Cynara scolymus 3 Dpt. Gen. Res. Veg. Chrysanthemum coronarium 1 Dpt. Gen. Res. Veg.

Brasicaceae Eruca sativa 14 OSEVA PRO Opava

Lepidium sativum 1 Dpt. Gen. Res. Veg.

Chenopodiaceae Spinacia oleracea 17 Dpt. Gen. Res. Veg.

Liliaceae Asparagus officinalis 17 Mendel Univ. Brno

Polygonaceae Rheum rhabarbarum 15 Mendel Univ. Brno

Tetragoniaceae Tetragonia tetragonoides 15 Dpt. Gen. Res. Veg.

Total 1571

Collection of the genus Lactuca L.(1408 acc.)

• Lactuca sativa (841 acc.) L. sativa L. 142 acc. L. sativa L. var. capitata L. 612 acc. L. sativa L. var. acephala Alef. 34 acc. L. sativa L. var. crispa L. 17 acc. L. sativa L. var. secalina Alef. 17 acc. L. sativa L. var. longifolia Lam. 17 acc. L. sativa L. var. angustana Irish ex Bremer 2 acc.

41, 5%

772, 92%

24, 3% 4, 0%

landraces cultivars breeding resources not determined

Collection of the genus Lactuca L.(1408 acc.)

• Wild Lactuca spp. (567 acc.) L. serriola 354 acc. L. saligna 90 acc. L. virosa 78 acc. other species (13) 35 acc. Lactuca spp. 6 acc. L. serriola x L. sativa 4 acc.

http://genbank.vurv.cz/genetic/resources/

Regeneration • L. sativa – isolation cages • Wild Lactuca spp. – greenhouse • 2006 – 2009: regenerated 218 acc. of leafy veg.

Species 2010 2011 2012 2013 Lactuca sativa 12 9 13 20 Lactuca spp. 8 7 3 12

Regeneration Species Available Regenerated % Lactuca sativa 841 785 93.3 Lactuca spp. 567 181 31.9 Cichorium endivia 26 9 34.6 Cichorium intybus 24 1 4.2 Cynara scolymus 3 0 0 Chrysanthemum

coronarium

1 1 100

Eruca sativa 14 14 100 Lepidium sativum 1 1 100 Spinacia oleracea 17 17 100 Asparagus officinalis 17 1 5.9 Rheum rhabarbarum 15 1 6.7 Tetragonia

tetragonoides

15 15 100

Maintenance

• Regenerated accessions are stored in the Dept. of Gene Bank of CRI in Prague under climatic conditions -18°C

• Accessions are also stored in Olomouc as a working collection under -20°C

• 39 Lactuca sativa cultivars (Czech varieties as Lednický, Safír, Smaragd, Kamýk, Valašský ...) are stored as safety

duplication in Slovak Gene Bank in Piešťany

Characterization and evaluation • National descriptor list for Lactuca sativa – 55 descriptors (Křístková et al. 2008) • National descriptor list for wild Lactuca sp. – 88 descriptors (Doležalová et al. 2002) • List of 26 basic descriptors for wild Lactuca sp. – prepared for the purposes of the

GENE-MINE project (Doležalová et al. 2003) • National descriptor list for Asparagus officinalis (18 desc.), Cichorium intybus (5

desc.), Cichorium endivia (6 desc.), Spinacia oleracea (7 desc.) (Chytilová et al. 2004)

• National descriptor list for Rheum rhabarbarum – 33 descriptors (Turečková et al.

2001)

Broadening of collection

Distribution of samples

• 2006 – 2009: 165 acc. of lettuce • 2010 – 2013: 68 acc. of L. sativa, 34 acc. of wild Lactuca spp., 11

acc. of rocket, 2 acc. of chicory (total 115)

• 2005 – 2013: 28 acc., recently not priority

Priorities

• Regeneration of wild Lactuca spp. (ca 60 % of collection) including taxonomical redetermination

• Regeneration of chicory collection (ca 60 % of collection) • Insert data on evaluation to EVIGEZ database

Thank you for your attention