Page 1 of 10Research Use Only

Cytometer Baseline Report

Cytometer: FACSAriaII User: BDServiceCytometer Name: FACSAriaII Institution: N/ASerial Number: 1 Software: BD FACSDiva 8.0.1Input Device: Manual Date: 01/07/2015 04:21 PMCytometer Configuration: 100 microns Standard

Setup Beads

Bead Product: CST Setup Beads Part #: 910858Lot ID: 24628 Expiration Date: 08/31/2015Bead Lot Information: Available

Detector Settings

Laser Detector Parameter PMTVNew Target

ValueOld Target

ValueBright Bead %Robust CV

Mid Bead Median Channel

Mid Bead %Robust CV

Dim Bead Median Channel

Dim Bead %Robust CV

Blue FSC FSC 367 125000 N/A 3.83 127210 3.74 15016 8.36

Blue F SSC 381 125000 N/A 4.69 121118 4.8 57305 4.73

Blue E FITC 636 29655 N/A 4.41 686 17.65 124 48.12

Blue D PE 586 44479 N/A 4.31 1003 17.39 208 46.57

Blue CPE-Texas

Red721 49988 N/A 5.17 1143 22.9 240 53.67

Blue B PerCP-Cy5-5 838 85836 N/A 6.09 3201 23.83 393 72.28

Blue A PE-Cy7 812 72140 N/A 7.88 2060 36.79 182 139.38

Red B APC 706 86986 N/A 5.51 3158 13.62 577 30.02

Red A APC-Cy7 685 76432 N/A 12.63 3113 17.36 335 42.88

Detector Settings (Continued)

Laser Detector ParameterLinearity Min

ChannelLinearity Max

ChannelSlope Intercept

Electronic Noise Robust

SDQr Br

Blue FSC FSC N/A N/A 0.0039 3.7 N/A N/A N/A

Blue F SSC N/A N/A 7.4216 -14.0 N/A N/A N/A

Blue E FITC 372 229069 7.4987 -16.6 46.7 0.0225 0

Blue D PE 314 232071 7.4817 -16.1 43.2 0.0904 46

Blue C PE-Texas Red 257 226378 7.5016 -16.7 51.2 0.0075 41

Blue B PerCP-Cy5-5 252 220112 7.5545 -17.1 52.7 0.0046 87

Blue A PE-Cy7 174 133655 7.7930 -17.8 50.1 0.0074 25

Red B APC 420 226380 7.5486 -16.6 42.1 0.0215 18

Red A APC-Cy7 281 156828 7.6465 -16.8 40.7 0.0026 527

Specifications

Red Laser Primary Channel Bright Bead %Robust CV: 6.00 (Recommended)Blue Laser Primary Channel Bright Bead %Robust CV: 6.00 (Recommended)

Laser Settings

LaserDelay

(Trigger on FSC)Delay

(Trigger on Fluorescence)Area Scaling Factor

Red -49.84 -49.32 0.91

Blue 0.00 0.00 1.09

Page 2 of 10Research Use Only

Cytometer Baseline Report

Cytometer: FACSAriaII User: BDServiceCytometer Name: FACSAriaII Institution: N/ASerial Number: 1 Software: BD FACSDiva 8.0.1Input Device: Manual Date: 01/07/2015 04:21 PMCytometer Configuration: 100 microns Standard

Window Extension: 2.00FSC Area Scaling Factor: 0.92

Threshold

Threshold (FSC): 12000

Fluidics

Sheath Pressure: 24.95 Sample Pressure: 23.79Nozzle Size: 100

Comments

Page 3 of 10Research Use Only

Cytometer Baseline Report

Cytometer: FACSAriaII User: BDServiceCytometer Name: FACSAriaII Institution: N/ASerial Number: 1 Software: BD FACSDiva 8.0.1Input Device: Manual Date: 01/07/2015 04:21 PMCytometer Configuration: 100 microns Standard

PMTV Optimization Results

Page 4 of 10Research Use Only

Cytometer Baseline Report

Cytometer: FACSAriaII User: BDServiceCytometer Name: FACSAriaII Institution: N/ASerial Number: 1 Software: BD FACSDiva 8.0.1Input Device: Manual Date: 01/07/2015 04:21 PMCytometer Configuration: 100 microns Standard

Page 5 of 10Research Use Only

Cytometer Baseline Report

Cytometer: FACSAriaII User: BDServiceCytometer Name: FACSAriaII Institution: N/ASerial Number: 1 Software: BD FACSDiva 8.0.1Input Device: Manual Date: 01/07/2015 04:21 PMCytometer Configuration: 100 microns Standard

Page 6 of 10Research Use Only

Cytometer Baseline Report

Cytometer: FACSAriaII User: BDServiceCytometer Name: FACSAriaII Institution: N/ASerial Number: 1 Software: BD FACSDiva 8.0.1Input Device: Manual Date: 01/07/2015 04:21 PMCytometer Configuration: 100 microns Standard

Page 7 of 10Research Use Only

Cytometer Baseline Report

Cytometer: FACSAriaII User: BDServiceCytometer Name: FACSAriaII Institution: N/ASerial Number: 1 Software: BD FACSDiva 8.0.1Input Device: Manual Date: 01/07/2015 04:21 PMCytometer Configuration: 100 microns Standard

Linearity Results

Page 8 of 10Research Use Only

Cytometer Baseline Report

Cytometer: FACSAriaII User: BDServiceCytometer Name: FACSAriaII Institution: N/ASerial Number: 1 Software: BD FACSDiva 8.0.1Input Device: Manual Date: 01/07/2015 04:21 PMCytometer Configuration: 100 microns Standard

Page 9 of 10Research Use Only

Cytometer Baseline Report

Cytometer: FACSAriaII User: BDServiceCytometer Name: FACSAriaII Institution: N/ASerial Number: 1 Software: BD FACSDiva 8.0.1Input Device: Manual Date: 01/07/2015 04:21 PMCytometer Configuration: 100 microns Standard

Page 10 of 10Research Use Only

Cytometer Baseline Report

Cytometer: FACSAriaII User: BDServiceCytometer Name: FACSAriaII Institution: N/ASerial Number: 1 Software: BD FACSDiva 8.0.1Input Device: Manual Date: 01/07/2015 04:21 PMCytometer Configuration: 100 microns Standard