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Robert Tampé ORCID: 0000-0002-0403-2160

Goethe University Frankfurt Professor of Biochemistry, Director tampe@em.uni-frankfurt.de Institute of Biochemistry, Biocenter Phone: +49-(0)69-798 29475 Max-von-Laue-Str. 9 Fax: +49-(0)69-798 29495 60438 Frankfurt/M., Germany www.biochem.uni-frankfurt.de Positions | Academic Training 2001-present Full Professor and Director, Institute of Biochemistry, Goethe University Frankfurt 1998-2001 Full Professor and Director, Physiological Chemistry, Medicine, University Marburg 1996-98 Assistant Professor of Biochemistry and Biophysics, TU Munich 1996 Habilitation in Biochemistry, TU Munich 1992-98 Independent Max Planck Research Group Leader, MPI of Biochemistry, Martinsried 1992-98 Research Group Leader, Department of Biophysics, TU Munich 1990-91 Max Kade Fellow, Stanford University (with Harden M. McConnell), USA 1987-89 PhD in Biochemistry with highest honors (summa cum laude), TU Darmstadt 1981-87 Diploma in Chemistry with highest honors (summa cum laude), TU Darmstadt

Scientific Awards | Honors (selection) 2019 Director, ISMB and Head of Joint Research Departments, University College London

(UCL) and Birkbeck College London (gratefully declined) 2019 Elected EMBO Member, Structural Biology, Membrane/Transport, Immunology 2018 Reinhart Koselleck Project Award, German Research Foundation (DFG) 2017 ERC Investigator, Advanced Grant, Life Sciences, European Research Council 2017-present Appointed Honorary Visiting Fellow, Merton College, University of Oxford, UK 2017-18 Visiting Professor, Department of Biochemistry, University of Oxford, UK 2010-present Award (3x), Excellence in Teaching, Goethe University Frankfurt 2010-present Honorary Professorship, iCeCM, Kyoto University, Japan 2009-10 Adjunct Professor, University of California San Francisco (UCSF), USA 1996-98 Heisenberg Fellow, German Research Foundation (DFG), Germany 1989-91 Postdoctoral Fellowship of DFG and Max Kade Foundation, New York, USA 1987-89 PhD Fellowship, awarded by the Chemical Industry (FCI), Germany 1985 Anton Keller Prize in Chemistry, Germany 1984-87 Fellow, Studienstiftung des Deutschen Volkes, Germany 1983-87 Fellow, Cusanuswerk, Germany

Academic Leadership (selection) 2008-20 Head of the Collaborative Research Center (CRC 807) Membrane Transport 2007-19 Cofounder, Board of Directors and PI, Cluster of Excellence Frankfurt (EXC 115) 2007-10 Spokesperson, Biomembranes, German Society Biochemistry and Molecular Biology 2005-10 Head of the European Membrane Biology Network (EMBN-Train) 2003-07 Head of the Collaborative Research Center (CRC 628) Membrane Proteomics 2001-16 Founder and Member of Board of Directors, Center for Membrane Proteomics (CMP)

Trusts | Editorial Boards (selection) 2020-present Board Member, EMBO Fellowships 2018-present Chair, ERC Advanced Grants, Life Sciences, European Research Council (ERC) 2016 Panel Member, Life Sciences, ERC Advanced Grants, European Research Council (ERC) 2015-16 Chair, Centre National de la Recherche Scientifique (CNRS), France 2014-present Trustee, German Research Foundation (DFG), Goethe University Frankfurt 2010-present Chair, Paul-Ehrlich Young Investigator Award, Paul Ehrlich Foundation 2008-11 Review Board Member, Basic Research in Biology and Medicine, DFG 2009 Review Board Member, Excellence Initiative, Germany 2003-08 Editorial Board, The Journal of Biological Chemistry (ASBMB) 2001-03 Editor of Faculty 1000

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Keynote Lectures | Organization of International Meetings (selection) 2023 Chair, Organizer, Gordon Research Conference, Membrane Transport, USA 2020 Arnold D. Welch Lecture, Yale University, New Haven, USA (postponed) 2019 Keynote Lecture, NWO CHAINS, The Dutch Chemistry Society, NL 2019 Baruch S. Blumberg Lecture (Nobel Prize 1976), Science Museum, Oxford, UK 2019 Keynote Lecture, Texas Center Membrane Protein Research Symposium, USA 2019 Keynote Lecture, FEBS International Conference, Budapest, Hungary 2018 Keynote Lecture, FEBS International Conference, Innsbruck, Austria 2017 Keynote Lecture, Symposium SFB 35, Vienna, Austria 2017 Plenary Lecture and Session Chair, IUBAP & EBSA Congress, Edinburgh, UK 2012 Chair, FASEB, Mechanism & Diseases of Transport ATPases, Aspen, USA 2011 Organizer, Int. Mol. Life Science Conference, GBM, Frankfurt, Germany 2010-present Organizer, International FEBS Conference, ABC Proteins, Innsbruck, Austria Publications >280 peer reviewed publications; 30 review articles; 4 patents; 15 editorials; 8 book articles Supervision of Graduate Students and Postdoctoral Fellows 1992-present 25 postdocs, 68 graduate students, >100 undergraduate students at Goethe

University Frankfurt, Philipps University Marburg, TU Munich, MPI of Biochemistry Current Teaching Activities and Involvement in Graduate Schools

• Bachelor and Master Biochemistry, Goethe University Frankfurt • Initiator, Integrative Graduate School Membrane Transport (TRAM) • Coordinator, International European Membrane Biology Training Network (EMBNTrain) • Member, DFG Graduate School Complex Light-triggered Reactions (CLiC) • Member, Int. Max Planck Research School for Heart and Lung Research (MPI-HLR) • Member, Int. Max Planck Research School for Structure and Function of Membranes (MPI-BP)

Other

• Additional Committee Memberships Member of numerous thesis advisory committees at the Goethe University Frankfurt, Max Planck Institute of Biophysics, Philipps University Marburg, TU Munich, and Max Planck Institute for Biochemistry; member of the selection committee for PhD fellowships of the Max Planck Research School. Member and reviewer of numerous advisory panels for the German Research Foundation DFG (including Excellence Clusters, Collaborative Research Centers, Priority and Graduates Programs) and for the Centre National de la Recherche Scientifique (CNRS, France). External member of evaluation boards for professorships in the USA, UK, Switzerland, Denmark, The Netherlands, Spain, and Germany. Chair of the evaluation panel in Life Sciences for ERC Advanced Grants of the European Research Council.

• Research Interests. Structure and mechanism of membrane proteins, macromolecular assemblies, molecular machines, intracellular transport and maturation of proteins, ribosome recycling and mRNA surveillance, opto/chemical biology, live-cell protein labeling techniques, nanobiotechnology.

• Reviewer Activities. Long-standing reviewer, but also editorial board membership, for a number of journals including Nature, Science, Science Advance, PNAS, EMBO J, Nat Struct Mol Biol, Nat Meth, Nat Chem Biol, Nat Communs, Nat Nanotechnol, Mol Cell, JBC, JACS, Angew Chem, eLife, and others.

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Major Achievements and Breakthroughs (2020-14)

• Transmembrane signaling by receptor confinement (2021 Science) • First structure of an archaeal ribosome quality control complex at 2.8 Å (2020 EMBO J) • Mechanistic basis of epitope proofreading and quality control by MHC I chaperones (2020 eLife) • Power-stroke mechanism of an ABC transporter by single-turnover analysis (2020 eLife) • Full conformational landscape of an ABC transporter under turnover conditions (2019 Nature) • Structure of the human MHC I peptide-loading complex (2017 Nature) • First X-ray structure of an MHC I chaperone and editing complex (2017 Science) • Structure of the post-splitting complex 40S-ABCE1 in ribosome recycling (2017 NSMB) • First high-resolution structure of a transport complex by single-particle cryo-EM (2015 Nature) • Native mass spectrometry of membrane protein complexes (2015 Nature Chem) • Single-cell based antigen compartmentalization in primary immune cells (2015 Nature Comm) • Mechanistic determinants of the antigen translocation complex TAP (2014 Nature Comm) • Inhibition mechanism of antigen processing by a poxviral immune evasin (2014 PLoS Pathogens)

Leadership in Large Research Networks

• Coordinator, Research Center CRC 807 – Membrane Transport and Communication • Cofounder, Board of Directors, Cluster of Excellence – Macromolecular Complexes • Section Head, European Drug Initiative on Channels and Transporters (EDICT), EU 7FP • Coordinator, European Membrane Biology Network (EMBNTrain), EU 7FR • Coordinator, Research Center CRC 628 – Functional Membrane Proteomics

Invited Lectures at International Conferences (2020-15, selection out of >250)

• Arnold D. Welch Lecture, Yale University, New Haven, USA, 2020 (postponed) • Keynote Lecture, NWO CHAINS, The Dutch Chemistry Society, NL, 2019 • Baruch S. Blumberg (Nobel Prize 1976) Lecture, Science Museum, Oxford, UK, 2019 • Keynote Lecture, Texas Center Membrane Protein Research Symposium, USA, 2019 • Keynote Lecture, FEBS International Conference, Budapest, Hungary, 2019 • EMBO Lecture, Antigen Processing and Presentation, Paris, 2019 • Gordon Research Conference, Archaea, Les Diablerets, Switzerland, 2019 • Keynote Lecture FEBS Symposium, Budapest, Hungary, 2019 • Annual Meeting of the Biophysical Society, Baltimore, USA, 2019 • Plenary Lecture, Conference of the Hellenic Crystallographic Association, Greece, 2018 • Plenary Lecture, Biochemical Society Conference, Birmingham, UK, 2018 • Bill and Melinda Gates Foundation Symposium, Oxford, UK, 2018 • Keynote Lecture, FEBS Symposium, Innsbruck, Austria, 2018 • IUBAP & EBSA International Biophysics Congress, Edinburgh, UK, 2017 • Gordon Research Conference: Membrane Transport, New London, NH, USA, 2017 • EMBO Conference, Chemical Biology, Heidelberg, Germany, 2016 • FASEB Conference: Molecular Biophysics of Membranes, Snow Mass, CO, USA, 2016 • Gordon Research Conference: Membrane Transport, Barga/Lucca, Italy, 2016 • International Cystic Fibrosis Conference, Washington D.C., USA, 2015 • Cold Spring Harbor Conference (CSH Asia), Shanghai/Suzhou, China, 2015

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10 Selected Publications (within the last 5 years) Google Scholar: >270 publications, 20,600 citations; h-index 76 (08/2021) | Research Gate | Research Loop

1) Sánchez MF, Els-Heindl S, Beck-Sickinger AG, Wieneke R, Tampé R (2021) Photo-induced receptor confinement drives ligand-independent GPCR signaling. Science, in press. (First Release in Science; Research Highlight in Nature Chemical Biology; Highlight in Faculty 1000 Recommended 9.8) – IF 41.8

2) Nürenberg-Goloub E, Kratzat H, Heilemann H, Heuer A, Kötter P, Berninghausen O, Becker T, Tampé R*,#, Beckmann R* (2020) Molecular analysis of the ribosome recycling factor ABCE1 bound to the 30S post-splitting complex. EMBO J, 39, e103788. (#lead contact, *corr. author) (Front Cover Story in EMBO J 2020) – IF 11.6

3) Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Brüchert S, Kuhn BT, Geertsma ER, Hummer G, Tampé R*,#, Moeller A* (2019) Conformation space of a heterodimeric ABC exporter under turnover conditions. Nature 471, 580-3. (#lead contact, *corr. author) – IF 42.8 (Front Cover Story in Nature 2019)

4) Blees A, Januliene D, Hofmann T, Koller N, Schmidt C, Trowitzsch S, Moeller A, Tampé R (2017) Structure of the human MHC-I peptide-loading complex. Nature 551, 525-8. – IF 42.8 (Front Cover Story and News & View in Nature 2017; Dispatch in Current Biology 2018; Highlight in F1000 2018; Viewpoint in Mol Immunol 2018; most cited paper (Web of Science)

5) Thomas C, Tampé R (2017) Structure of the TAPBPR-MHC I complex defines the mechanism of peptide loading and editing. Science 358, 1060-4. – IF 41.8 (Insights and Perspectives in Science 2017; News & View in Nature 2017; Highlight in F1000 2018; Viewpoint in Biochemistry 2018)

6) Heuer A, Gerovac M, Schmidt C, Trowitzsch S, Preis A, Kötter P, Berninghausen O, Becker T, Beckmann R, Tampé R (2017) Structure of the 40S-ABCE1 post-splitting complex in ribosome recycling and translation initiation. Nat Struct Mol Biol 24, 453-60. – IF 15.4 (Cover Page in NSMB; Preview in Mol Cell 2017)

7) Nöll A, Thomas C, Herbring V, Zollmann T, Barth K, Mehdipour AR, Tomasiak TM, Brüchert S, Joseph B, Abele R, Olieric V, Wang M, Diederichs K, Hummer G, Stroud RM, Pos KM, Tampé R (2017) Structure and mechanistic basis of a functional homolog of the antigen transporter TAP. Proc Natl Acad Sci USA 114, E438-E447. – IF 11.2

8) Lehnert E, Mao J, Mehdipour AR, Hummer S, Abele R, Glaubitz C, Tampé R (2016) Antigenic peptide recognition on the human ABC transporter TAP resolved by DNP-enhanced solid-state NMR spectroscopy. J Am Chem Soc 138, 13967-74. – IF 15.4

9) Kollmannsperger A, Sharei A, Raulf A, Heilemann M, Langer R, Jensen KF, Wieneke R, Tampé R (2016) Live-cell protein labelling with nanometre precision by cell squeezing. Nature Commun 7, 10372. – IF 14.9

10) Kim JM, Wu, S, Tomasiak T, Mergel C, Winter MN, Stiller S, Robles-Colmanares Y, Stroud RM*, Tampé R*, Craik CS*, Cheng Y* (2015) Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter. Nature 517, 396-400. (*corr. author) – IF 42.8

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Selected Reviews (within the last 3 years)

1) Thomas C, Tampé R (2020) Structural and mechanistic principles of ABC transporters. Annu Rev Biochem 89, 605-36.

2) Trowitzsch S, Tampé R (2020) Multifunctional chaperone and quality control complexes in adaptive immunity. Annu Rev Biophys,49, 135-161.

3) Wieneke R, Tampé R (2019) Multivalent chelators for in vivo protein labeling. Angew Chem 58, 8278-90.

4) Gerovac M, Tampé R (2018) Control of mRNA translation by versatile ATP-driven machines. Trends Biochem Sci 44, 167-80.

5) Abele R, Tampé R (2018) Moving the cellular peptidome by transporters. Fronts Cell Dev Biol 6, 43.

6) Thomas C, Tampé R (2017) Proofreading of peptide-MHC complexes through dynamic multivalent interactions. Front Immunol 8, 65.

Selected Collaborative Work

1) Barbet G, Nair-Gupta P, Schotsaert M, Yeung ST, Moretti J, Seyffer F, Metreveli G, Tardner T, Choi A, Tortorella D, Tampé R, Khanna KM, Garcia-Sastre A, Blander JM (2021) TAP dysfunction redirects subcellular MHC-I traffic to enable non-canonical cross-presentation and CD8 T cell priming. Nat Immunol 22, 497-509.

2) Khoo KK, Galleano I, Gasparri F, Wieneke R, Harms H, Poulsen MH, Chua HC, Wulf M, Tampé R, Pless SA (2020) Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing. Nature Communications 11, 2284.

3) Guesdon A, Bazile F, Buey R, Mohan R, Monier S, Rodríguez-García R, Angevin M, Heichette C, Wieneke R, Tampé R, Duchesne L, Akhmanova A, Steinmetz M, Chrétien D (2016) EB1 interacts with outwardly curved and straight regions of the microtubule lattice. Nature Cell Biology 8, 1102-8.

4) Kaur H, Lakatos-Karoly A, Vogel R, Nöll A, Tampé R, Glaubitz C (2016) Coupled ATPase-adenylate kinase activity in ABC transporters. Nature Communications 7, 13864.

5) Bechara C, Nöll A, Morgner, N, Degiacomi, MT, Tampé R*, Robinson CV* (2015) A subset of annular lipids is linked to the flippase activity of an ABC transporter. Nature Chemistry 7, 255-62. (*corr. author)

6) Pfreundschuh M, Alsteens D, Wieneke R, Zhang C, Coughlin S, Tampé R, Kobilka B, Müller D (2015) Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFM. Nature Communications 6, 8857.

7) Zollmann T, Moiset G, Tumulka F, Tampé R, Poolman B, Abele R (2015) Single liposome analysis of peptide translocation by the ABC transporter TAPL. Proc Natl Acad Sci USA 112, 2046-51

8) Nair-Gupta P, Baccarini A, Tung N, Seyffer F, Florey O, Huang, Y, Banerjee M, Overholtzer M, Roche PA, Tampé R, Brown DB, Amsen D, Whiteheart SW, Blander JM (2014) TLR signals induce phagosomal MHC-I delivery from endosomal recycling compartment to allow cross-presentation. Cell 185, 506-14.

9) Rossier O, Octeau V, Sibarita JB, Leduc C, Tessier B, Nair D, Gatterdam V, Destaing O, Albiges-Rizo C, Tampé R, Cognet L, Choquet D, Lounis B, Giannone G. (2012) Integrins function as homotypic stable adhesion units transiently trapped in adhesion sites. Nature Cell Biology 14, 1057-1067

10) Burgdorf S, Schölz C, Kautz A, Tampé R, Kurts C (2008) Spatial and mechanistic separation of cross-presentation and endogenous antigen presentation. Nature Immunology 9, 558-66.