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CurriculumVitae TereseM.Bergfors(néeHirth)
Dept.ofCellandMolecularBiology,BiomedicalCenter,Box596UppsalaUniversity,SE‐75124Uppsala,SwedenDateofBirth:January29,1955Telephone:+46(18)471‐4543Fax:+46(18)536971E‐mail:[email protected]:http://xray.bmc.uu.se/terese SynopsisMy area of expertise is protein crystallization. I am part of the structural biologygroup of Prof. Alwyn Jones at Uppsala University where I have headed thecrystallizationactivitiessince1984. AsoneoftheeightPrincipal InvestigatorsonagrantfundedbytheSwedishFoundationforStrategicResearch,Iexpress,purify,andcrystallize tuberculosis proteins and work on the development of crystallizationmethodology.Education 1974B.A.inNaturalSciences,BellarmineCollege,LouisvilleKentucky,
USAEmploymentHistorySince1992 Department of Cell and Molecular Biology, Biomedical Center, Box
596,UppsalaUniversity,Sweden(HeadResearchEngineer)1984‐1991 DepartmentofMolecularBiology,BiomedicalCenterBox590,
Uppsala University,Sweden1982‐1984 CellMotilityGroup,StockholmUniversity,Sweden1980‐1981 DepartmentofPathology,UppsalaUniversity,Sweden1977‐1980 Biology Department, Southern Methodist University, Dallas Texas,
USA Consultancies2007 Microlytic,A/S;www.microlytic.com1992‐1996 AstraHässle,Mölndal,SwedenOther NovozymesA/S,Denmark;KaroBio,Stockholm;Bioventia,Uppsala.ProfessionalActivitiesSince2006 Co‐editor,ActaCrystallographicaFSince2004 Scientific Advisory Board, International Organization for Biological
Crystallization(IOBC)2007 GuestCo‐editor,ICCBM11Proceedings,JournalofCrystalGrowth
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2007 Thesisdefensecommittee:MichaelNielsen,“Expression,Purificationand Characterization of Tryptophan Hydroxylases”, Dept. ofChemistry,TheTechnicalUniversityofDenmark.
2005 Thesisdefensecommittee“Cristalizacióndeproteínasdeuncampoeléctricointerno”NuritMirkin,Dept.ofChemistry,UNAM,MexicoCity.
PublicationsBooksBergfors, T., Editor. Protein Crystallization: Second Edition. 2009. InternationalUniversityLine,LaJolla,California,500pp.Bergfors,T.,Editor.ProteinCrystallization:Techniques,Strategies,andTips.1999.InternationalUniversityLine,LaJolla,California,300pp.Journalarticlesorbookchapters20081. Bergfors, T. The RAPID crystallization strategy for structure‐based inhibitor
design. Integrating Crystallography in Drug Discovery. Editor: Sussman, J.SpringerPress,1‐10.
2. Ericsson,D.,Kasrayan,A.,Johansson,P.,Bergfors,T.,Sandström,A,Bäckvall,J‐E,Mowbray, S. X‐ray structure ofCandida antarctica lipase A shows a novel lidstructureandalikelymodeofinterfacialactivation.J.Mol.Biol.376,109‐119.
3. Covarrubias, A. S., Högbom, M., Bergfors, T., Carroll, P., Mannerstedt, K.,
Oscarson,S.,Parish,T., Jones,T.A.,Mowbray,S.Structural,biochemical,and invivo investigationsof thethreoninesynthase fromMycobacteriumtuberculosis.J.Mol.Biol.381,622‐633.
20074. Ubhayasekera,W.,Tang,C.M.,Ho,S.,Berglund,G.,Bergfors,T.,Chye,M‐L,and
Mowbray, S. Crystal structures of a family 19 chitinase from Brassica junceashowflexibilityofbindingcleftloops.FEBSLetters.274,3695‐3703.
5. Bergfors,T.Automatedliquid‐handlingsystemsforsubmicrolitercrystallization.
Protein Crystallization for Structural Genomics. Ed. Chayen, N. InternationalUniversityLine,LaJolla,California.
6. Bergfors,T.Succeedingwithseeding:somepracticaladvice.EvolvingMethods
forMacromolecularCrystallography. Editors:Read,R.&Sussman, J. SpringerPress,1‐10.
20067. Bergfors, T. Crystallization strategy at the Uppsala University RAPID Center.
SynchrotronRadiationScience&Technology13,5‐9.
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8. Bergfors, T. Screening and optimization methods for non‐automatedcrystallizationlaboratories.MethodsMol.Biol.363,131‐151.
20059. Covarrubias,A.,Larsson,A.,Högbom,M.,Lindberg,J.,Bergfors,T.,Björkelid,C.,
Mowbray, S., Unge, T. and Jones, T.A. Structure and function of carbonicanhydrassesfromMycobacteriumtuberculosis.J.Biol.Chem.280,18782‐18789.
10. Johansson, P., Unge, T., Cronin, A., Arand, M., Bergfors, T., Jones, T.A. and
Mowbray, S. Structure of an atypical epoxide hydrolase fromMycobacteriumtuberculosisgivesinsightsintoitsfunction.J.Mol.Biol.351,1048‐1056.
11. Larsson,A.,Bergfors,T.,Dultz,E., Irwin,D.,Roos,A.,Driguez,H,Wilson,D.and
Jones, T.A. Crystal structure of Thermobifida fusca endoglucanase Cel6A incomplexwithsubstrateand inhibitor: the roleof tyrosineY73 in substrate ringdistortion.Biochemistry44,12915‐12922.
200412. Roos,A.,Anderson,C.E.,Bergfors, T., Jacobsson,M,Karlen,A,Unge, T., Jones,
T.A., and Mowbray, S. Mycobacterium tuberculosis ribose‐5‐phosphateisomerasehasaknownfold,butanovelactivesite.J.Mol.Biol.335,799‐809.
200313. Bergfors,T.SeedstoCrystals.J.Struct.Biol.142,66‐76.14. Johansson, P. Denman, S., Brumer, H., Kallas, Å., Henriksson, H., Bergfors, T.,
Teeri, T., and Jones, T.A. Crystallization and preliminary X‐ray analysis of axyloglucanendotransglycosylasefromPopulustremulaxtremuloides.ActaCrystD59,535‐537.
15. Jakobsson,E.,Alvite,G.,Bergfors,T., Esteves,A., andKleywegt,G. Thecrystal
structureofEchinococcusgranulosus fatty‐acid‐bindingprotein1.BBA‐ProteinsandProteonomics49,40‐50.
16. Arand,M.,Hallberg,B.,Zou,J.,Bergfors,T.,Oesch,F.,vanderWerf,M.,deBont,
J., Jones, T. & Mowbray, S. 1.2 Å structure of Rhodococcus erythropolislimonene‐1,2‐epoxidehydrolaserevealsanovelmechanism.EMBOJ.22,2583‐2592.
17. Arand,M.,Cronin,A.,Oesch,F.,Hallberg,M.,Zou,JY,Bergfors,T.,Mowbray,S.,
andJones,T.A.Thetell‐talestructuresofepoxidehydrolases.DrugMetabolismReviews35:7Suppl.1.
2002‐198418. Van Aalten, D.M.F.,Milne, K.G., Zou, J.Y., Kleijwegt, G., Bergfors, T., Ferguson,
M.A.J.,Knudsen,J.andJones,T.A.Bindingsitedifferencesrevealedbycrystal
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structures of Plasmodium falciparum and bovine Acyl‐CoA binding protein. J.Mol.Biol.(2001)309,181‐192.
19. Zou,J.,Hallberg,B.M.,Bergfors,T.,Oesch,F.,Arand,M.,Mowbray,S.andJones,
T.A. Structure of Aspergillus niger epoxide hydrolase at 1.8Å resolution:implicationsforthestructureandfunctionofthemammalianmicrosomalclassofepoxidehydrolases.Structure(2000)8,111‐122.
20. Hallberg, B.M., Bergfors, T., Backbro, K., Pettersson, G., Henriksson, G., and
Divne, C. A new scaffold for binding haem in the cytochrome domain of theextracellularflavocytochromecellobiosedehydrogenase.Structure(2000)8,79‐88.
21. Chaudhuri,B.N.,Kleywegt,G.J.,Broutin‐L'Hermite, I., Bergfors,T., Senn,H., Le
Motte, P., Partouche, O., and Jones, T.A. Structures of cellular retinoic acidbindingproteinsIandII incomplexwithsyntheticretinoids. ActaCryst.(1999)D55,1850‐1857.
22. Kleywegt,G.J.,Bergfors,T.,Senn,H.,LeMotte,P.,Gsell,B.,Shudo,K.,andJones,
T.A. Crystal structures of cellular retinoid acid binding proteins I and II incomplexwith all‐trans retinoic acid and a synthetic retinoid. Structure (1994)2:1241‐1258.
23. Bergfors,T.,Kleywegt,G.J.,andJones,T.A. CrystallizationandX‐rayanalysisof
recombinant bovine cellular retinoic acid binding protein. Acta Cryst. (1994)D50,370‐374.
24. Uppenberg, J., Patkar, S., Bergfors, T., and Jones, T.A. Crystallization and
preliminary studies of lipase B fromCandida antarctica . J.Mol. Biol. (1994)235,790‐792.
25. Kuehn, M.J., Ogg, D.J., Kihlberg, J., Slonim, L. Flemmer, K., Bergfors, T., and
Hultgren,S.StructuralbasisofpilussubunitrecognitionbythePapDchaperone.Science(1993)272,1234‐1241.
26. Rouvinen,J.,Bergfors,T.,Teeri,T.andJones,A.Thethreedimensionalstructure
ofthecoreproteinofcellobiohydrolaseII.Science(1990)249,380‐386.27. Jones,T.A.,Cowan,S.,Newcomer,M.andBergfors,T. Crystallographicstudies
ontwofamiliesofretinoidbindingproteins.FrontiersinDrugResearch,AlfredBenzonSymposium28,Munksgaard,Cophenhagen.(1990).
28. Cowan, S., Bergfors, T., Jones, T.A., Tibbelin, G., Olin, B., Board, P., and
Mannervik, B. Crystallization of GST2, a human class alpha glutathionetransferase.J.Mol.Biol.(1989)208,369‐370.
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29. Bergfors,T.,Rouvinen, J., Lehtovaara,P.,Caldentey,X.,Tomme,P.,Claeyssens,M.,Pettersson,G.,Teeri,T.,Knowles,J.andJones,T.A.CrystallizationofthecoreproteinofcellobiohydrolaseIIfromTrichodermareesei.J.Mol.Biol.(1989)209,167‐169.
30. Jones,T.A.,Bergfors,T.,Sedzik,J.andUnge,T.Thethreedimensionalstructure
ofP2myelinprotein.EMBOJ.(1988)7,1597‐1604.31. Sedzik,J.,Bergfors,T.,Jones,T.A.andWeise,M.BovineP2myelinbasicprotein
crystallizesinthreedifferentforms.J.Neurochem.(1988)50,1908‐1913.32. Bergfors, T., Sedzik, J., Unge, T., Fridborg, K., Weise, M. and Jones, T.A.
CrystallizationofP2myelinprotein.J.Mol.Biol.(1987)198,357‐358.33. Hultcrantz,R.,Ericsson,J.andHirth,T.Levelsofmalondialdehydeproductionin
ratliverfollowingloadingandunloadingwithiron.VirchowsArch(CellPathol)(1984)45,135‐146.
34. Abok, K.,Hirth, T., Ericsson, J., andBrunk,U. Effect of iron on the stability of
macrophagelysosomes.VirchowsArch(CellPathol)(1983)43,85‐101.Chairmanshipatinternationalconferences
• 2007 Co‐chair, Microsymposium on Protein Crystallization, 24th EuropeanCrystallizationMeeting,Marrakkesh,Morocco
• 2004 Sessionchair,Tenth InternationalConferenceon theCrystallizationofBiologicalMacromoleculesCrystallizationCourse,Bejing,China
• 2003 Chairman of the Microsymposium on Protein Crystallization, 21stEuropeanCrystallizationMeeting,Durban,SouthAfrica
Acceptedinvitationsasaspeaker(internationalvenues)2008
• ICCBM12(InternationalWorkshopandConferenceontheCrystallizationofBiologicalMacromolecules12),Cancun,Mexico
• InternationalSchoolofCrystallography,Erice,Italy• IUCr,InternationalUnionofCrystallographyXXIIIWorldCongress,Osaka,
Japan• Dept.ofChemistry,UniversityofTokyo,Tokyo,Japan• PEPC6(ProteinExpression,Purification,&Crystallization6),EMBOCourse,
Hamburg,Germany• AdvancedMethodsinCrystallizationIII,FEBSAdvancedCourse(Federation
ofBiologicalSciences),NoveHrady,CzechRepublic• AustralianCourseinMacromolecularCrystallization,CSIRO(Commonwealth
ScientificandIndustrialResearchOrganization),Melbourne,Australia.• ProteinCrystallizationSymposium,InstituteforMolecularBioscience,
UniversityofQueensland,Brisbane,Australia
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2007
• XIVCongresoIbéroamericanodeCristalografía,Caracas,Venezuela• ProteasePlatform,Novartis,Basel,Switzerland• Hauptmann‐Woodward Medical Research Institute Lecture Series, Buffalo,
NY• 14thCCP4NorthProteinStructureWorkshop,Carlisle,UK
2006
• UniversityofCopenhagen,Dept.ofChemistry• 11th International Conference on the Crystallization of Biological
MacromoleculesCrystallizationCourse,Quebec,CanadaPLENARYSPEAKER• British Crystallographic Association SpringMeeting,University of Lancaster,
UK• CentroNacionaldeInvestigacionesOncológicas(CNIO),Madrid,Spain• InternationalSchoolonBiologicalCrystallization,Granada,Spain• FEBS Course Advanced Methods in Macromolecular Crystallization, Nove
Hrady,CzechRepublic.• EMBO Practical Course in: Protein Expression, Purification, and
Crystallization(PEPC5),EMBL,Hamburg,Germany.
2005• Advances inProteinScienceMeeting,TheChineseUniversityofHongKong,
China.• DepartmentofChemistry,UNAM,MexicoCity,Mexico.• EvolvingMethodsinMacromolecularCrystallography,InternationalSchoolof
Crystallography,Erice,Sicily,Italy.PLENARYSPEAKER
2004• FEBS Course Advanced Methods in Macromolecular Crystallization, Nove
Hrady,CzechRepublic.• EMBO Practical Course in: Protein Expression, Purification, and
Crystallization(PEPC4),EMBL,Hamburg,Germany.• Tenth International Conference on the Crystallization of Biological
MacromoleculesCrystallizationCourse,Bejing,China.
2003• KeynotespeakerattheSixthHeartofEuropeanbio‐CrystallographyMeeting,
Wittenberg,Germany.• AmericanCrystallographicAssociationAnnualMeeting,Cincinnati,OhioUSA.
2002
• EMBO Practical Course in: Protein Expression, Purification, andCrystallization(PEPC3),EMBL,Hamburg,Germany.
• Ninth International Conference on the Crystallization of BiologicalMacromoleculesCrystallizationCourse,JenaGermany.
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2001• RecentAdvancesinMacromolecularCrystallization,LeBischenberg,France.• First Southeast Asian Protein Crystallography Workshop, Suranaree
UniversityofTechnology,NakhonRatchasima,Thailand
2000andpreviousyears• 2000 EMBO course in Protein Expression, Purification, and Crystallization,
Hamburg,Germany• 1999XVIIIMeetingof the InternationalUnionofCrystallography,Glasgow,
Scotland• l997 Recent Advances in Macromolecular Crystallization, Le Bischenberg,
France• 1994 Workshop on Protein Crystallization, Dept. of Organic Chemistry,
TechnicalUniversity,Budapest,Hungary• 1993 Fifth International Conference on the Crystallization of Biological
Macromolecules,SanDiego,USATeachingOrganizerofyearlygraduatelevelcourses
• PracticalProteinCrystallizationGraduateCourse,UppsalaUniversity• WritingaScientificJournalArticleinEnglish,UppsalaUniversity
LecturerforothercoursesinScandinavia• Crystallography Course, Dept. of Chemistry, University of Copenhagen,
Denmark.• Protein Structures Course,UmeåCenter forMolecular Pathogenesis,Umeå
University,Sweden.• Protein Technology Course, Swedish Agricultural University, Uppsala,
Sweden.• Advanced Course in Structural Biochemistry and Bioinformatics, Stockholm
University,Sweden.Prizes2001 "Best practical crystallization tip" at Recent Advances in
MacromolecularCrystallization,LeBischenbergFrance.1997 “Best oral presentation” at Recent Advances in Macromolecular
Crystallization,LeBischenbergFrance.1997 “Best practical crystallization tip” at Recent Advances in
MacromolecularCrystallization,LeBischenbergFrance.