CURRICULUM VITAE - Gavin Edmund REID

Associate Professor Department of Chemistry

Department of Biochemistry and Molecular Biology Michigan State University

229 Chemistry Building East Lansing, MI, USA, 48824-1322

Phone: 517-355-9715x198

Fax: 517-353-1793 Email: reid@chemistry.msu.edu

http://www.chemistry.msu.edu/faculty/reid/main.html D.O.B: June 10, 1969 BIOGRAPHICAL SKETCH:

Gavin E. Reid is an Associate Professor in the Department of Chemistry and the Department of Biochemistry and Molecular Biology at Michigan State University. Over the past 25 years, he has held a variety of technical research positions and academic appointments in the USA and Australia. From 1987-1997 he worked as a Research Assistant with Prof. Richard Simpson in the Joint Protein Sequencing Laboratory at the Ludwig Institute for Cancer Research in Melbourne, Australia, while studying part time to obtain an Associate Diploma in Applied Science. He then obtained a Post Graduate Diploma in Science in 1997 and a PhD in Chemistry in 2000 from the University of Melbourne, under the joint supervision of Prof. Richard O’Hair and Prof. Simpson. From 2000-2002, he carried out post-doctoral research with Prof. Scott McLuckey at Purdue University, prior to establishing his independent research career as an Assistant Member at the Ludwig Institute for Cancer Research. He then moved to Michigan State in 2004 as an Assistant Professor, where he was promoted to the rank of Associate Professor with tenure in 2009.

Research in the Reid laboratory at Michigan State is broadly focused on (i) the development of novel chemical, bio-analytical and mass spectrometry based methods and instrumentation for proteome and lipidome analysis, and (ii) the application of these strategies to identify biomarkers for the onset and progression of disease. To date, this work has resulted in 125 peer reviewed publications (50 as an independent researcher), presentations at 65 national and international conferences, 40 invited seminars and 3 patents. Funding to pursue this research has been received from a variety of state and federal agencies, including the National Institutes of Health, the National Science Foundation, the Australian Research Council, the Multiple Myeloma Research Foundation, and several industry groups. Gavin is currently a member of the editorial advisory boards of the Journal of the American Society for Mass Spectrometry and the European Journal of Mass Spectrometry. He has served as an ad hoc study section panel member to several national and international funding agencies, and is a regular reviewer for multiple analytical chemistry, protein chemistry and mass spectrometry journals. EDUCATION: 1998-2000 PhD. School of Chemistry. University of Melbourne.

“Studies on the fragmentation of amino acids and peptides by tandem mass spectrometry and gas-phase ion-molecule reactions” Advisors: Dr Richard A.J. O’Hair and Professor Richard J. Simpson.

1997 Post Graduate Diploma in Science. School of Chemistry. University of

Melbourne. “A mass spectrometric and ab initio study of the pathways for dehydration of simple glycine and cysteine containing peptide [M+H]+ ions” Advisors: Dr Richard A.J. O’Hair and Professor Richard J. Simpson.

1990-1991 Associate Diploma of Applied Science - (Laboratory Technology). Swinburne

College of TAFE. PROFESSIONAL APPOINTMENTS: 2009 - Associate Professor. Department of Chemistry. Michigan State University. East

Lansing, MI, USA. 2009 - Associate Professor. Department of Biochemistry and Molecular Biology.

Michigan State University. East Lansing, MI, USA. 2004 - 2009 Assistant Professor. Department of Chemistry. Michigan State University. East

Lansing, MI, USA. 2004 - 2009 Assistant Professor. Department of Biochemistry and Molecular Biology.

Michigan State University. East Lansing, MI, USA. 2002 - 2004 Assistant Member. Joint Proteomics Laboratory. The Ludwig Institute for Cancer

Research. Parkville, Victoria, Australia. 2000 - 2002 Post Doctoral Research Associate. Purdue University. Department of

Chemistry. West Lafayette, Indiana, USA. Mentor: Professor Scott A. McLuckey.

1987 – 1998 Junior Research Assistant / Research Assistant / Research Officer / Senior

Research Officer. Joint Protein Structure Laboratory. The Ludwig Institute for Cancer Research and The Walter and Eliza Hall Institute for Medical Research, Parkville, Victoria, Australia.

HONORARY APPOINTMENTS: 2004 Honorary Senior Fellow. Department of Surgery. University of Melbourne.

Parkville, Victoria, Australia 2003 - 2004 Honorary Fellow. School of Chemistry. University of Melbourne. Parkville,

Victoria, Australia PROFESSIONAL DUTIES / SERVICE:

Departmental:

Dept. of Chemistry. Michigan State University.

• Graduate Admissions Committee (Fall 2007 – Spring 2009, Fall 2010 – Spring 2012) • Faculty Advisory Committee (Fall 2005 – Spring 2007) • Chair, Analytical Faculty Search Committee (Fall 2006) • Analytical Chemistry Seminar Coordinator (Spring 2007, Fall 2008 - Fall 2009) • Member, Membrane Protein Chemistry Faculty Search Committee (Fall 2011)

Dept. of Biochemistry and Molecular Biology. Michigan State University.

• Student Judiciary Committee (2008 - )

Ludwig Institute for Cancer Research • Member, Postgraduate Appointment and Review Committee (2003 - 2004) • Seminar Program Coordinator (2003 - 2004)

University/Local/National/International:

Michigan State University:

• University Hearing Board, Michigan State University (Fall 2008 – Fall 2009) • All University Traffic and Transportation Committee, Michigan State University (Spring

2012 – present) American Chemical Society – Michigan State University Local Section

• Treasurer, (2011 – present) • Chair (2007 - 2009)

American Society for Mass Spectrometry

• Board of Directors, Member at Large for Education (2012 – 2014) • Instructor, Ion Trap Mass Spectrometry Short Course (2008 – 2010) • Chair, Ion Trap Mass Spectrometry Interest Group (2007 - 2008) • Chair, Peptide Fragmentation Interest Group. (2010 - 2011)

Michigan Mass Spectrometry Discussion Group

• Chair, (2006 - 2010) Conference Session Chair:

• ‘Peptides with PTM’s’. 23rd ASMS Sanibel Conference, St. Pete Beach, FL (January 2011)

• ‘Innovations in Bioanalytical Mass Spectrometry’. 2009 Anachem/SAS Symposium, Livonia, MI (November 2009)

• ‘Mass Spectrometry of Lipids’. 57th Conference of the American Society for Mass Spectrometry, Philadelphia, PA (June 2009) • ‘Technologies for Biology’. 14th Lorne Proteomics Symposium, Lorne, Victoria,

Australia (February 2009) • ‘Role of Mass Spectrometry in Biomarker Discovery, Development, and Clinical Studies’. LabAutomation 2009, Palm Springs, CA (January 2009) • ANACHEM award symposium honoring Prof. Scott McLuckey. 35th Conference of the

Federation of Analytical Chemistry and Spectroscopy Societies. Reno, NV (September 2008)

• ‘Mass Spectrometry’. 2007 Anachem/SAS Symposium, Livonia, MI (November 2007) • ‘Applications in Ion Trap Mass Spectrometry’. 55th Conference of the American Society for Mass Spectrometry, Indianapolis, IN (June 2007)

Member, Editorial Board:

• Journal of the American Society for Mass Spectrometry. (2011 – present) • Scientific Reports (Nature Publishing group) (2011 – present) • European Journal of Mass Spectrometry. (2008 – present) • The Open Spectroscopy Journal, Open Spectroscopy Reviews, Open Spectroscopy Letters (Bentham Science Publishers) (2007 – 2011)

Conference Organizing Committees:

• Co-chair, 2009 Midwestern Universities Analytical Chemistry Conference. Michigan State University.

• Program Committee. 58th Conference of the American Society for Mass Spectrometry (2010, 2011) • 19th Conference of the Australian and New Zealand Society for Mass Spectrometry. (2003) • Program Committee International Representative, Lorne Proteomics Symposium.

Australasian Proteomics Society, Australia (2011 – present) Ad hoc Review Panel Member:

• Early Detection Research Network - Biomarker Discovery Laboratories, Scientific Review Group, National Cancer Institute. (May 2010)

• Enabling Bioanalytical and Biophysical Technologies Study Section. National Institutes of Health. (October 2009)

• National Research Council, Associateship Programs Life Sciences Review Panel (2009, 2011) • Special Emphasis Scientific Review Group. National Cancer Institute. (July 2006, March 2007, June 2007, October 2008) • Instrumentation and Systems Development Study Section. National Institutes of Health. (October 2006, February 2007)

Ad hoc Reviewer:

• National Science Foundation. Division of Chemistry. (March 2007) • Discovery Projects Research Proposal. Australian Research Council (May 2007, May 2009) • Cottrell College Science Award Proposal. Research Corporation. (June 2007)

Manuscript Reviewer:

• Analytical Chemistry, Analyst, Biotechnology Progress, BioTechniques, Chemical

Communications, Experimental Eye Research, FEBS Journal, International Journal of Mass Spectrometry, International Journal of Molecular Sciences, Journal of the American Chemical Society, Journal of the American Society for Mass Spectrometry, Journal of Mass Spectrometry, Journal of Natural Products, Journal of Neurochemistry, Journal of Physical Chemistry, Journal of Proteome Research, Mass Spectrometry Reviews, Nature, Physical Chemistry Chemical Physics, Proteomics, Rapid Communications in Mass Spectrometry.

SOCIETY MEMBERSHIPS:

• American Society for Mass Spectrometry • American Chemical Society • Australian and New Zealand Society for Mass Spectrometry • Australasian Proteomics Society

AWARDS / SCHOLARSHIPS: 2011 Bowie Medal, Australian and New Zealand Society for Mass Spectrometry 2008 Michigan State University College of Natural Sciences Teacher-Scholar Award 2007 American Society for Mass Spectrometry Research Award 2006 National Science Foundation CAREER award Sigma Xi Meritorious Junior Faculty Award (Michigan State University Chapter) 2002 Victorian Young Tall Poppy Award – Australian Institute of Policy and Science 2001 The Monica Reum Memorial Prize. School of Chemistry. University of Melbourne. 1999 University of Melbourne Travel Abroad Scholarship Best Student Oral Presentation Award- 17th Conference of the Australian and New

Zealand Society for Mass Spectrometry 1998-2000 Australian Post Graduate Research Award 1998 Dixson Research Scholarship in Chemistry. University of Melbourne. Student Poster Presentation Prize - 22nd Lorne Conference on Protein Structure

and Function. 1997 RACI Industry Sponsored Student Presentation Award 1985-1986 James Fairnie Scholarship for Chemistry TEACHING Michigan State University: Semester Year Course Course Description Fall 2012 CEM 434 (Scheduled) Analytical Chemistry (Undergraduate) (40 lectures, 3 credits) Spring 2012 CEM832 Mass Spectrometry (Graduate) (40 lectures, 3 credits) Fall 2011 CEM 434 Analytical Chemistry (Undergraduate) (40 lectures, 3 credits) Fall 2010 CEM834 Advanced Analytical Chemistry – Electronics module (Graduate) (13 lectures, 3 credits) Spring 2010 CEM832 Mass Spectrometry (Graduate) (40 lectures, 3 credits) Fall 2009 CEM834 Advanced Analytical Chemistry – Electronics module

(Graduate) (13 lectures, 3 credits) CEM938 Analytical Seminar (Graduate) Spring 2009 BMB495 Undergraduate Seminar (Sophomore) (1 lecture, 2 credits) CEM938 Analytical Seminar (Graduate) Fall 2008 CEM 835 Advanced Analytical Chemistry - Mass Spectrometry module (Graduate) (13 lectures, 3 credits) BMB101 Frontiers in Biochemistry (Freshman seminar) (1 lecture, 1 credit) CEM938 Analytical Seminar (Graduate) Spring 2008 CEM832 Mass Spectrometry (Graduate) (40 lectures, 3 credits) Fall 2007 CEM 434 Analytical Chemistry (Undergraduate) (40 lectures, 3 credits) Spring 2007 CEM938 Analytical Seminar (Graduate seminar) BMB495 Undergraduate Seminar (Sophomore) (1 lecture, 2 credits) Fall 2006 CEM 434 Analytical Chemistry (Undergraduate) (40 lectures, 3 credits) BMB101 Frontiers in Biochemistry (Freshman seminar) (1 lecture, 1 credit) Spring 2006 CEM 832 Mass Spectrometry (Graduate) (40 lectures, 3 credits) Fall 2005 CEM 434 Analytical Chemistry (Undergraduate) (40 lectures, 3 credits) BMB829 Methods of Macromolecular Analysis and Synthesis (Graduate) (5 lectures, 2 credits) Spring 2005 CEM 832 Mass Spectrometry (Graduate) (40 lectures, 3 credits) University of Melbourne: Year Course Course Description 2004 610-497 Structure Elucidation by Mass Spectrometry (Graduate) School of Chemistry (10 lectures) 2003 521-322 Protein Biochemistry and Proteomics (Undergraduate) Department of Biochemistry (3 lectures) 2003 610-497 Structure Elucidation by Mass Spectrometry (Graduate) School of Chemistry (10 lectures) 2002 521-322 Protein Biochemistry and Proteomics (Undergraduate) Department of Biochemistry (3 lectures) GRADUATE / UNDERGRADUATE STUDENT ADVISOR Ph.D. and M.S. advisees: Current: Ms Cassie Fhaner (3rd yr, PhD) Ms Li Cui (3rd yr, PhD) Mr Shuai Nie (1st yr, PhD)

Ms Kristen Reese (1st yr, PhD) Ms Fatmata Jollah (1st yr, PhD)

Previous:

Dr Xiao Zhou (PhD, 2012) Dr Yali Lu (PhD, 2010) Ms Sichang Liu (MSc, 2010) Mr Zachary Keltner (MSc, 2009)

Dr Amanda Palumbo (PhD, 2009) Dr Xi Zhang (PhD, 2009) Dr Jennifer Froelich (PhD, 2008) Ms Yali Lu (MSc, 2007) Ms Gwynyth Scherperel (MSc, 2006) Mr Mahasilu Amunugama (MSc, 2006)

Undergraduate Research Students: Current:

Ms Caitlin Mester (Fall 2011 - ) Mr Steven Townsend (Spring 2012-) Previous: Mr Kyle Safran (Fall 2009 – Fall 2010)

Mr David Broome (Fall 2009) Mr James Sierakowski (Fall 2004 - Spring 2006) Mr Colin Blakely (Fall 2006) Ms Emily Blanco (Fall 2006) Ms Katherine Rank (Spring 2005 - Fall 2008) Mr Matthew Ruiz (Fall 2007 - Spring 2009) Ms Rebecca Renis (Spring 2008 - Spring 2009)

Ms Jaya Gupta (Fall 2009 – Spring 2010) Ms Olivia Hudson (Fall 2011)

Post Doctoral Research Associate: Current:

Dr Todd Lydic (Summer 2012 - )

Previous: Dr Yali Lu (Fall 2010 – Spring 2011) Dr Scott Smith (Spring 2009 – Fall 2010) Dr Jennifer Froelich (Spring 2009) Dr Kade Roberts (Fall 2004 – Spring 2005)

Other: 2007 Student Mentor. High School Honors Science/Mathematics/Engineering Program.

Michigan State University. 2002 Undergraduate Student advisor. School of Chemistry. University of Melbourne. 2002 High School Science Student Mentor. Dept. Education and Training Victoria. FUNDING Current: 1. National Institutes for Health. RO1 RR025386

Mass Spectrometry Strategies to Identify Lipid Biomarkers of Disease. Gavin E. Reid (PI), Julia Busik (co-PI) and Rheal Towner (subaward co-PI). Funding Period: 07/31/2009-08/01/2013.

This proposal involves the development and application of mass spectrometry methods to monitor changes in lipid abundances as potential markers for the onset and progression of disease in animal models of diabetic retinopathy and hepatocellular carcinoma.

2. National Institutes of Health. RO1 EY016077

Dyslipidemia and Diabetic Retinopathy Julia Busik (PI), Gavin Reid (co-PI) and Walter Esselman (co-PI) Funding Period 12/01/2010 - 11/30/2015. The major goal of this project is to determine the role of fatty acids in the development of diabetic retinopathy in type 2 diabetes.

3. Dow Chemical. Advancing Mass Spectrometry Analysis with Fundamental Gas-Phase Ion Chemistries of

Analytes. Gavin Reid (PI). Funding Period: 01/20/2010 - 01/19/2012 (no cost extension to 08/31/2012) The major goal of this proposal is to develop improved chemical derivatization and tandem mass

spectrometry based analysis strategies for lipid analysis. Completed: 1. National Science Foundation (CHE-0547940)

CAREER: Chemical methods for selective proteome analysis. Gavin E. Reid (PI) Funding period: 02/01/2006 - 01/31/2011 (no cost extension to 07/31/2011) (8.3% annual effort) 0 academic, 1.0 summer

2. Multiple Myeloma Research Foundation.

Phosphoproteomic analysis of NF-kB signaling in multiple myeloma. Jetze Tepe (PI), Merlin Bruening (co-PI), Gavin E. Reid (co-PI), Ronald Henry (co-PI). Funding Period: 06/01/2008 – 5/30/2010.

3. 21st Century Jobs Fund. Michigan Economic Development Corporation.

Application of laser-based molecular scalpel technology for proteomic and metabolomic analysis. Marcus Dantus (PI), A. Dan Jones (co-PI) and Gavin E. Reid (co-PI).

Funding Period: 07/01/2006 – 06/30/2009. 4. Michigan State University. Health and Biomedical Research Initiative II (06-HBRI-639)

Cancer Proteomics Center of Excellence. Jetze Tepe (PI), Merlin Bruening (co-PI), Ronald Henry (co-PI), John Lapres (co-PI), Thomas Pinnavaia (co-PI) and Gavin E. Reid (co-PI).

Funding Period: 01/07/2006 – 06/30/2009. 5. American Society for Mass Spectrometry Research Award.

Cross-linking reagents and multistage tandem mass spectrometry for the analysis of protein-protein interactions. Gavin Reid (PI).

Funding period: 08/01/2007 - 07/31/2008.

6. Agilent Technologies Inc.

Mass spectrometry instrumentation for 'top down' proteomics. Gavin Reid (PI). Funding Period: 02/17/2005 - 02/17/2008.

7. Dow Chemical. Unrestricted research in bioanalytical mass spectrometry. Gavin Reid (PI).

Funding Period: 01/08/2007 - 01/07/2008. 8. Michigan State University. Intramural Research Grant Program (04-IRGP-117)

Integrating 'bottom-up' and 'top-down' mass spectrometry approaches to quantitative proteome analysis. Gavin Reid (PI).

Funding Period: 12/15/2004 - 06/15/2006. 9. GE Healthcare.

Development of "modular" stable isotope labelled derivatization reagents for quantitative protein analysis by ion trap mass spectrometry. Gavin Reid (PI).

Funding Period: 2/1/2005 - 7/31/2005. 10. Australian Research Council. (DP0342771)

Differential Expression Proteomics: Identification and quantitation of peptides and proteins by fixed charge derivatization and tandem mass spectrometry. Gavin Reid (PI).

Funding Period: 01/01/2003 – 06/30/2004. (Note: this ARC funding was awarded for 3 years but was relinquished after 18 months due to my relocation to the USA in June 2004).

PUBLICATIONS: (H-index = 34; total citations without self citations = 4,134 (05/08/12)) † Corresponding Author Associate Professor - Michigan State University (2009 – ) †125. Zhang, X, Fhaner, C.J., Ferguson-Miller, S.M. and Reid, G.E. (2012) Evaluation of ion activation

strategies and mechanisms for the gas-phase fragmentation of sulfoquinovosyldiacylglycerol lipids from Rhodobacter sphaeroides. Int. J. Mass Spectrom. 316-318: 100-107.

Invited article to ‘Alex Harrison 80th Birthday’ Special Issue.

124. Kalcic, C.L., Reid, G.E., Lozovoy, V.V. and Dantus, M. (2012) Mechanism Elucidation for Non-

Stochastic Femtosecond Laser-Induced Ionization/Dissociation: from Amino Acids to Peptides. J. Phys. Chem. 116: 2764-2774. PMID: 22141398

123. Mathivanan, S., Fhaner, C.J., Reid, G.E. and Simpson, R.J. (2012) ExoCarta 2012: database of

exosomal proteins, RNA and lipids. Nucleic Acids Res. 40: D1241-D1244. PMID: 21989406 †122. Lu, Y., Zhou, X., Stemmer, P.M. and Reid, G.E. (2012) Sulfonium Ion Derivatization, Isobaric Stable

Isotope Labeling and Data Dependent CID- and ETD-MS/MS for Enhanced Phosphopeptide

Quantitation, Identification and Phosphorylation Site Characterization. J. Am. Soc. Mass Spectrom. 23: 577-593. PMID: 21952753

Invited article to ‘2011 ASMS Sanibel Conference’ and ‘Bela Paizs, Biemann Medal’ Special Issue.

121. Opreanu, M., Tikhonenko, M., Bozack, S., Lydic, T.A., Reid, G.E., McSorley, K.M., Sochacki, A.,

Perez, G.I., Esselman, W.J., Kolesnick, R., Grant, M.B. and Busik, J.V. (2011) The unconventional role of acid sphingomyelinase in regulation of retinal microangiopathy in diabetic human and animal models. Diabetes. 60: 2370-2378. PMID: 21771974

†120. Palumbo, A.M., Smith, S.A., Kalcic, C.L., Dantus, M., Stemmer, P.M. and Reid, G.E. (2011)

Tandem Mass Spectrometry Strategies for Phosphoproteome Analysis. Mass Spectrom. Rev. 30: 600-625. PMID: 21294150

119. Zhang, X., Hiser, C., Tamot, B., Benning, C., Reid, G.E. and Ferguson-Miller, S.M. (2011)

Combined Genetic and Metabolic Manipulation of Lipids in Rhodobacter sphaeroides Reveals Non-phospholipid Substitutions in Fully Active Cytochrome c Oxidase. Biochemistry. 50: 3891-3902. PMID: 21476580

118. Zhang, X., Tamot, B., Hiser, C., Reid, G.E., Benning, C. and Ferguson-Miller, S.M. (2011)

Cardiolipin-Deficiency in Rhodobacter sphaeroides Alters the Lipid Profile of Membranes and of Crystallized Cytochrome Oxidase, but Structure and Function are Maintained. Biochemistry. 50: 3879-3890. PMID: 21476578

†117. Smith, S.A., Kalcic, C.L., Safran, K.A., Stemmer, P.M., Dantus, M. and Reid, G.E. (2010) Enhanced

Characterization of Singly Protonated Phosphopeptide Ions by Femtosecond Laser-induced Ionization/Dissociation Tandem Mass Spectrometry (fs-LID-MS/MS). J. Am. Soc. Mass Spectrom. 21: 2031-2040. PMID: 20888783

116. Griffitts, J., Saunders, D., Tesiram, Y.A., Reid, G.E., Salih, A., Liu, S., Lydic, T.A., Busik, J.V., Kang,

J.X. and Towner, R. (2010) Non-Mammalian FAT-1 Gene Prevents Neoplasia when Introduced to a Mouse Hepatocarcinoma Model. Biochim. Biophys. Acta. Mol. Cell Biol. Lipids. 1801: 1133-1144. PMID: 20620224

†115. Zhou, X., Lu, Y., Wang, W., Borhan, B. and Reid, G.E. (2010) ‘Fixed Charge’ Chemical

Derivatization and Data Dependant Multistage Tandem Mass Spectrometry for Mapping Protein Surface Residue Accessibility. J. Am. Soc. Mass Spectrom. 21: 1339-1351. PMID: 20452239

Invited article to ‘2009 ASMS Distinguished Contribution in Mass Spectrometry award’ Special Issue.

†114. Wang, W-H., Palumbo, A.M., Tan, Y-J., Reid, G.E., Tepe, J.J. and Bruening, M.L. (2010)

Identification of p65-associated Phosphoproteins by Mass Spectrometry after On-plate Phosphopeptide Enrichment Using Polymer-oxotitanium Films. J. Prot. Res. 9: 3005-3015. PMID: 20380454

113. Opreanu, M., Lydic, T.A., Reid, G.E., McSorley, K.M., Esselman, W.J. and Busik, J. (2010)

Inhibition of cytokine signaling in human retinal endothelial cells through downregulation of sphingomyelinases by docosahexaenoic acid. Invest. Ophthalmol. Vis. Sci. 51: 3253-3263. PMID: 20071681

†112. Keltner, Z., Meyer, J.A., Johnson, E.M., Palumbo, A.M., Spence D.M and Reid, G.E. (2010) Mass

Spectrometric Characterization and Activity of Zinc Activated Proinsulin C-peptide and C-peptide Mutants. The Analyst.135: 278-288. PMID: 20098759

111. Tikhonenko, M., Lydic, T.A., Wang, Y., Chen, W., Opreanu, M., Sochacki, A., McSorley, K.M., Renis, R.L., Kern, T., Jump, D.B., Reid, G.E. and Busik, J.V. (2010) Remodeling of Retinal Fatty Acids in a Animal Model of Diabetes: a Decrease in Long Chain Polyunsaturated Fatty Acids is Associated with a Decrease in Fatty Acid Elongases Elovl2 and Elovl4. Diabetes. 59: 219-27. PMID: 19875612

110. Dunn, J.D., Reid, G.E. and Bruening, M.L. (2010) Techniques for phosphopeptide enrichment prior

to analysis by mass spectrometry. Mass Spectrom. Rev. 29: 29-54. PMID: 19263479 Assistant Professor - Michigan State University (2004 – 2009) †109. Lydic, T.A., Renis, R., Busik, J.V. and Reid, G.E. (2009) Analysis of Retina and Erythrocyte

Glycerophospholipid Alterations in a Rat Model of Type 1 Diabetes. JALA. 14: 383-399. PMID: 20161420

Invited article to ‘The Best of Lab Automation 2009’ Special Issue.

108. Meyer, J.A., Subasinghe, W., Sima, A.F.A., Keltner, Z., Reid, G.E., Daleke, D. and Spence, D.M.

(2009) Zinc-activated C-peptide Resistance to the Type 2 Diabetic Erythrocyte is Associated with Hyperglycemia-induced Phosphatidylserine Externalization and Reversed by Metformin. Mol. Biosystems. 5: 1157-1162. PMID: 19756305

†107. Froelich, J.M., Lu, Y. and Reid, G.E. (2009) Chemical Derivatization and Multistage Tandem Mass

Spectrometry for Protein Structural Characterization. In: Practical Aspects of Trapped Ion Mass Spectrometry. Vol. 5: Applications. (R.E. March and J.F.J. Todd. Ed), CRC Press.

Invited book chapter.

†106. Busik, V.M., Reid, G.E. and Lydic, T.A. (2009) Global Analysis of Retinal Lipids by Complementary

Precursor Ion and Neutral Loss Mode Tandem Mass Spectrometry. Meth. Mol. Biol. 579: 33-70. PMID: 19763470

105. Frawley Cass, S.M., Reid, G.E. and Tepe, J.J. (2009) Synthesis of diazo ketone solid supports

and their application towards the enrichment of phosphorylated peptides. Org. Biomol. Chem. 7: 3291 - 3299. PMID: 19641788

104. Scherperel, G., Reid, G.E. and Waddell Smith, R. Characterization of Smokeless Powders Using

Nanoelectrospray Ionization Mass Spectrometry (nESI-MS). (2009) Anal. Bioanal. Chem. 394: 2019-2028. PMID: 19242680

†103. Lydic, T.A., Busik, J.V. Esselman, W.J. and Reid, G.E. Complementary Precursor Ion and Neutral

Loss Scan Mode Tandem Mass Spectrometry for the Comprehensive Analysis of Phosphatidylethanolamine Lipids from Whole Rat Retina. (2009) Anal. Bioanal. Chem. 394: 267-275. PMID: 19277613

Invited article to ‘Young Investigators in Analytical and Bioanalytical Science’ Special Issue.

102. Griffitts, J., Tesiram, Y., Reid, G.E., Saunders, D., Floyd, R. and Towner, R. (2009) In Vivo

magnetic resonance spectroscopy (MRS) assessment of altered fatty acyl unsaturation in liver tumor formation of a TGFα/c-myc transgenic mouse model. J. Lipid. Res. 50: 611-622. PMID: 19065002

†101. Froelich, J.M. and Reid, G.E. (2009) The Effect of Post-translational and Process-induced

Modifications on the Multistage Gas-Phase Fragmentation Reactions of Protonated Peptide Ions. Combinatorial Chemistry and High Throughput Screening. 12: 175-184. PMID: 19199885

Invited review to ‘New Frontiers in Biological Mass Spectrometry’ special issue. †100. Kalcic, C.L., Gunaratne, T., Jones, A.D., Dantus, M. and Reid, G.E. Femtosecond Laser-induced

Ionization/Dissociation of Protonated Peptides. (2009) J. Am. Chem. Soc. 131: 940-942. PMID: 19128059

Highlighted as a ‘Science and Technology Concentrates’ article in ‘Chemical and Engineering News’ (2009), 87(3), p57.

†99. Zhang, X., Ferguson-Miller, S.M. and Reid, G.E. (2009) Characterization of Ornithine and

Glutamine Lipids Extracted from Cell Membranes of Rhodobacter sphaeroides. J. Am. Soc. Mass Spectrom. 20: 198-212. PMID: 18835523

†98. Palumbo, A.M and Reid, G.E. (2008) Evaluation of Gas-Phase Rearrangement and Competing

Fragmentation Reactions on Protein Phosphorylation Site Assignment using CID-MS/MS and MS3. Anal. Chem. 80: 9735-9747. PMID: 19012417

†97. Lu, Y., Tanasova, M., Borhan, B. and Reid, G.E. (2008) An Ionic Reagent for Controlling the Gas-

Phase Fragmentation Reactions of Cross-Linked Peptides. Anal. Chem. 80: 9279-9287. PMID: 19551991

†96. Froelich, J.M., Kaplinghat, S. and Reid, G.E. (2008) Automated Neutral Loss and Data Dependent

Energy Resolved “Pseudo MS3” for the Targeted Identification, Characterization and Quantitative Analysis of Methionine-Containing Peptides. Eur. J. Mass Spectrom. 14: 219-229. PMID: 18756020

95. Dunn, J.D., Igrisan, E.A., Palumbo, A.M., Reid, G.E. and Bruening, M.L. (2008) Phosphopeptide

Enrichment Using MALDI Plates Modified with High-capacity Polymer Brushes. Anal. Chem. 80: 5727-5735. PMID: 18578546

94. Hohmann, L.J., Eng, J.K., Gemmill, A., Klimek, J., Vitek, O., Reid, G.E. and Martin D. B. (2008)

Quantification of the Compositional Information Provided by Immonium Ions on a Quadrupole-TOF Mass Spectrometer. Anal. Chem. 80: 5596-5606. PMID: 18564857

†93. Froelich, J.M. and Reid, G.E. (2008) The Origin and Control of Process-Induced Oxidative Peptide

Modifications Prior to Mass Spectrometry Analysis. Proteomics. 8: 1334–1345. PMID: 18306178 †92. Palumbo A.M., Tepe, J,J. and Reid, G.E. (2008) Mechanistic Insights into the Multistage Gas-

Phase Fragmentation Behavior of Phosphoserine- and Phosphothreonine-containing Peptides. J. Prot. Res. 7: 771 - 779. PMID: 18181561

91. Meyer, J., Froelich, J.M., Reid, G.E., Karunarathne, W. and Spence, D. (2008) Metal-activated C-

peptide Facilitates Glucose Clearance and the Release of a Nitric Oxide Stimulus via the GLUT1 Transporter. Diabetologia. 51: 175-182. PMID: 17965850

90. Lioe, H., Laskin, J., Reid, G.E. and O’Hair, R.A.J. (2007) Energetics and Dynamics of the

Fragmentation Reactions of Protonated Peptides containing Methionine Sulfoxide or Aspartic Acid via Energy- and Time-Resolved Surface Induced Dissociation. J. Phys. Chem. A. 111: 10580-10588. PMID: 17914758

†89. Froelich J.M. and Reid, G.E. (2007) Mechanisms for the Proton Mobility Dependant Gas-Phase

Fragmentation Reactions of S-alkyl Cysteine Sulfoxide-containing Peptide Ions. J. Am. Soc. Mass Spectrom. 18: 1690-1705. PMID: 17689096

†88. Lioe, H., O'Hair, R.A.J. Gronert, S., Austin, A. and Reid, G.E. (2007) Experimental and Theoretical Proton Affinities of Methionine, Methionine Sulfoxide and their N- and C-terminal Derivatives. Int. J. Mass Spectrom. 267: 220-232.

†87. Scherperel, G. and Reid, G.E. (2007) Emerging methods in Proteomics: Multistage Tandem Mass

Spectrometry for Top-down Protein Characterization. The Analyst. 132: 500-506. PMID: 17525804

Invited review to Education i-section. Highlighted Research Article in ‘Chemical Biology’. (2007), vol 7.

†86. Sierakowski, J., Amunugama, M., Roberts, K.D. and Reid, G.E. (2007) Substituent Effects on the

Fragmentation Reactions of Methionine Side Chain Fixed Charge Phenacylsulfonium Ion Containing Peptides. Rapid Commun. Mass Spectrom. 21: 1230-1238. PMID: 17330214

†85. Roberts, K.D. and Reid, G.E. (2007) Leaving Group Effects on the Selective Gas Phase

Fragmentation Reactions of Cysteine Side Chain Fixed Charge Containing Peptide Ions. J. Mass Spectrom. 42: 187-198. PMID: 17154347

†84. Amunugama, M., Roberts, K.D. and Reid, G.E. (2006) Mechanisms for the Selective Gas-phase

Fragmentation Reactions of Methionine Side Chain Fixed Charge Sulfonium Ion Containing Peptides. J. Am. Soc. Mass Spectrom. 17: 1631-1642. PMID: 16935522

†83. Wang, Y., Scherperel, G., Roberts, K.D., Jones, A.D., Reid, G.E. and Yan, H. (2006) A Point

Mutation Converts Dihydroneopterin Aldolase to a Cofactor-Independent Oxygenase. J. Am. Chem. Soc. 128: 13216-13223. PMID: 17017801

†82. Zhang, X., and Reid, G.E. (2006) Multistage Tandem Mass Spectrometry of Anionic

Phosphatidylcholine Lipid Adducts Reveals Novel Dissociation Pathways. Int. J. Mass Spectrom. 252: 242-255.

†81. Scherperel, G., Yan, H., Wang, Y. and Reid, G.E. (2006) 'Top-down' characterization of site-

directed mutagenesis products of Staphylococcus aureus dihydroneopterin aldolase by multistage tandem mass spectrometry in a linear quadrupole ion trap. The Analyst. 131: 291-302. PMID: 16440096

Invited article to 'Emerging Investigators' special issue.

Assistant Member - Ludwig Institute for Cancer Research (2002 – 2004) †80. Reid, G.E., Roberts, K.D., Simpson, R.J., O'Hair, R.A.J. (2005) Selective Identification and

Quantitative Analysis of Methionine Containing Peptides by Charge derivatization and Tandem mass spectrometry. J. Am. Soc. Mass Spectrom. 16: 1131-1150.

†79. Reid, G.E., Roberts, K.D., Kapp, E.A. and Simpson, R.J. (2004) Statistical and Mechanistic

Approaches to Understanding the Gas-phase Fragmentation Behaviour of Methionine Sulfoxide Containing Peptides. J. Prot. Res. 3: 751-759.

†78. Lioe, H., O’Hair, R.A.J. and Reid, G.E. (2004) A Mass Spectrometric and Molecular Orbital Study

of H2O Loss from Tryptophan and Oxidized Tryptophan Derivatives. Rapid Commun. Mass Spectrom. 18: 978-988.

†77. Lioe, H., O’Hair, R.A.J. and Reid, G.E. (2004) Gas-Phase Reactions of Protonated Tryptophan. J.

Am. Soc. Mass Spectrom. 15: 65-76.

†76. Reid, G.E. Characterization of Proteins by Mass Spectrometry. (2003) In: 'Purifiying Proteins: A Laboratory Manual' (Simpson, R.J. Ed.) Cold Spring Harbor Laboratory Press. Chapter 21, pp 489-516.

Invited book chapter. 75. Kapp, E.A., Schütz, F., Reid, G.E., Eddes, J.S., Moritz, R.L., O’Hair, R.A.J., Speed, T.P. and

Simpson, R.J. (2003) Mining a tandem mass spectrometry database to determine the trends and global factors influencing peptide fragmentation. Anal. Chem. 75: 6251-6264.

Postdoctoral Research Associate – Purdue University (2000 – 2002) 74. Pitteri, S., Reid, G.E. and McLuckey, S.A. (2004) Affecting Proton Mobility in Activated Peptides

and Whole Protein Ions via Lysine Guanidination. J. Prot. Res. 3: 46-54. 73. Reid, G.E., Wells, J.M., Badman, E.R. and McLuckey S.A. (2003) Performance of a quadrupole

ion trap mass spectrometer adapted for ion-ion reaction studies. Int. J. Mass Spectrom. 222: 243-258.

†72. Reid, G.E. and McLuckey, S.A. (2002) "Top down" protein identification and characterization via

tandem mass spectrometry. In: 'Proteomics: A Laboratory Manual' by Simpson, R.J. Cold Spring Harbor Laboratory Press.

Invited contribution. 71. He, M, Reid, G.E. Shang, H-S., Lee, G.U. and McLuckey, S.A. (2002) Product ion formation from

multiple parent ion charge states following gas-phase protein ion purification and concentration. Anal. Chem. 74: 4653-4661.

70. Reid, G.E. and McLuckey, S.A. (2002) “Top down” protein characterization via tandem mass

spectrometry. J. Mass Spectrom. 37: 663-675. 69. Reid, G.E., Shang, H., Hogan, J., Lee, G.U. and McLuckey S.A. (2002) Gas-phase concentration,

purification and identification of whole proteins from complex mixtures. J. Am. Chem. Soc. 124: 7353-7362.

68. Engel, B.J. Pan, P. Reid, G.E., Wells, J.M. and McLuckey, S.A. (2002) Charge state dependent

fragmentation of gaseous protein ions in a quadrupole ion trap: Bovine ferri-, ferro-, and apo-cytochrome c. Int. J. Mass Spectrom. 219: 171-187.

67. Stephenson, J.L., McLuckey, S.A., Reid, G.E., Wells, J.M. and Bundy, J.L. (2002) Ion/ion

chemistry as a top-down approach for protein analysis. Curr. Opin. Biotechnol. 13: 57-64. †66. Reid, G.E. and McLuckey, S.A. (2002) Tandem mass spectrometry of ribonuclease A and B: N-

linked glycosylation site analysis of whole protein ions. Anal. Chem. 74: 577-583. 65. McLuckey, S.A. Reid, G.E. Wells, J.M. (2002) Ion parking during ion/ion reactions in

electrodynamic ion traps. Anal. Chem. 74: 336-346. 64. Newton, K.A., Chrisman, P.A., Reid, G.E., Wells, J.M. and McLuckey, S.A. (2001) Gaseous

apomyoglobin ion dissociation in a quadrupole ion trap: [M+4H]4+ - [M+21H]21+. Int. J. Mass Spectrom. 212: 359-376.

63. Chrisman, P.A., Newton, K.A., Reid, G.E., Wells, J.M. and McLuckey, S.A. (2001) Loss of

charged versus neutral heme from gaseous holomyoglobin ions. Rapid. Commun. Mass Spectrom. 15: 2334-2340.

62. Wells, J.M., Reid, G.E., Engel, B.J., Pan, P. and McLuckey, S.A. (2001) Dissociation reactions of

gaseous ferro-, ferri-, and apo-cytochrome c ions. J. Am. Soc. Mass Spectrom. 12: 873-876. 61. Reid, G.E., Wu, J., Chrisman, P.A., Wells, J.M. and McLuckey, S.A. (2001) Charge state

dependance on the sequence analysis of protonated ubiquitin ions by tandem mass spectrometry and ion/ion reactions. Anal. Chem. 73: 3274-3281.

Post Graduate Diploma in Science (1997) / PhD – University of Melbourne (1998 – 2000) 60. Farrugia, J.M., O'Hair, R.A.J. and Reid, G.E. (2001) Do all b2 ions have oxazolone structures?

Results from multistage mass spectrometry and ab initio studies on protonated N-acyl amino acid model systems. Int. J. Mass. Spectrom. 210/211: 71-87.

†59. Reid, G.E., Tichy, S.E., Pérez, J., O'Hair, R.A.J., Simpson, R.J. and Kenttämaa, H.I. (2001) N-

terminal derivatization and fragmentation of neutral peptides via ion-molecule reactions with acylium ions: Towards gas-phase Edman degradation? J. Am. Chem. Soc. 123: 1184-1192.

58. Reid, G.E., Simpson, R.J. and O’Hair, R.A.J. (2000) Leaving group and gas phase neighboring

group effects in the side chain losses from protonated serine and its derivatives. J. Am. Soc. Mass Spectrom. 11: 1047-1060.

57. O’Hair, R.A.J., Androutsopoulos, N.K. and Reid, G.E. (2000) Do amines react with protonated

peptides in the gas phase via transacylation reactions to induce peptide bond cleavage? Rapid. Commun. Mass Spectrom. 14: 1707-1716.

56. O’Hair, R.A.J. and Reid, G.E. (2000) The search for stable gas phase b1 ions derived from

aliphatic amino acids: A combined experimental and ab initio study. Rapid. Commun. Mass Spectrom. 14: 1220-1225.

55. O’Hair, R.A.J. and Reid, G.E. (2000) Derivatization of protonated peptides via gas phase ion

molecule reactions with acetone. J. Am. Soc. Mass Spectrom. 11: 244-256. 54. Vrkic, A.K., O’Hair, R.A.J., Foote, S. and Reid, G.E. (2000) Fragmentation reactions of all 64

protonated trimer oligonucleotides and 16 mixed base tetramer oligonucleotides via tandem mass spectrometry in an ion trap. Int. J. Mass Spectrom. 194: 145-164.

53. O’Hair, R.A.J. and Reid, G.E. (1999) Neighboring group versus cis elimination mechanisms for

side chain loss from protonated methionine, methionine sulfoxide and their peptides. Eur. Mass Spectrom. 5: 325–334.

52. Reid, G.E., Simpson, R.J. and O’Hair, R.A.J. (1999) Probing the fragmentation of protonated

glycine oligomers via multistage mass spectrometry and gas phase ion molecule reactions. Int. J. Mass Spectrom. 190/191: 209-230.

51. Reid, G.E., O’Hair, R.A.J., Styles, M.L., McFadyen, W.D., and Simpson, R.J. (1998) Gas phase

ion-molecule reactions in a modified ion trap: H/D exchange of non-covalent complexes and coordinatively unsaturated platinum complexes. Rapid Commun. Mass Spectrom. 12: 1701-1708.

50. O’Hair, R.A.J., Styles, M.S. and Reid, G.E. (1998) The role of the sulfhydryl group on the gas

phase fragmentation reactions of protonated cysteine and cysteine containing peptides. J. Am. Soc. Mass Spectrom. 9: 1275-1284

49. O’Hair, R.A.J. and Reid, G.E. (1998) Does side chain water loss from protonated threonine yield

N-protonated dehydroamino-2-butyric acid? Rapid Commun. Mass Spectrom. 12: 999-1002.

48. Reid, G.E., Simpson, R.J. and O'Hair, R.A.J. (1998) A mass spectrometric and ab initio study of the pathways for dehydration of simple glycine and cysteine containing peptide [M+H]+ ions. J. Am. Soc. Mass Spectrom. 9: 945-956.

Junior Research Assistant / Research Assistant / Research Officer / Senior Research Officer – Ludwig Institute for Cancer Research (1987 – 1998) 47. Elso, C., Toohey, B., Reid, G.E., Poetter, K., Simpson, R.J. and Foote, S.J. (2002) Mutation

detection using mass spectrometry separation of tiny oligonucleotide fragments. Genome Research. 12: 1428-1433.

46. Verhagen, A.M., Ekert, P.G., Pakusch, M., Silke, J., Connolly, L.M., Reid, G.E., Moritz, R.L.,

Simpson, R.J. and Vaux, D.L. (2000) Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins. Cell. 102: 43-53.

45. Simpson, R.J. Connelly, L.M., Eddes, J.S., Pereira, J.J., Moritz, R.L. and Reid, G.E. (2000)

Proteomic analysis of the human colon carcinoma cell line (LIM 1215): Development of a membrane protein database. Electrophoresis. 21: 1707-1732.

44. Cole, A.R., Hall, N.E., Trautlein, H.R., Eddes, J.S., Reid, G.E., Moritz, R.L. and Simpson, R.J.

(1999) Disulfide bond structure and N-glycosylation sites of the extracellular domain of the human interleukin-6 receptor. J. Biol. Chem. 274: 7207-7215.

43. Moss, J., Reid, G.E., Mullin, K.A., Zawadzki, J.L., Simpson, R.J. and McConville, M.J. (1999)

Characterization of a novel GDP-mannose:serine-protein mannose-1-phosphotransferase from Leishmania mexicana. J. Biol. Chem. 274: 6678-6688.

42. Simpson, R.J. and Reid, G.E. (1998) Sequence analysis of gel-resolved proteins. Chapter 8 In:

Gel electrophoresis of proteins: A practical approach (3rd Edition) Hames, B.D. (Ed). Oxford University Press.

41. Reid, G.E., Rasmussen, R.K., Dorow, D.S. and Simpson, R.J. (1998) Capillary column

chromatography improves sample preparation for mass spectrometric analysis: Complete characterisation of human α enolase from two-dimensional gels following in-situ proteolytic digestion. Electrophoresis. 19: 946-955.

40. Rasmussen, R.K., Ji, H., Eddes, J.S., Zugaro, L.M., Reid, G.E., Simpson, R.J. and Dorow, D.S.

(1998) Two-dimensional gel database of human breast carcinoma cell expressed proteins: an update. Electrophoresis. 19: 818-825.

39. Rassmussen, R,K., Ji, H., Eddes, J.S., Moritz, R.L., Reid, G.E., Simpson, R.J. and Dorow, D.S.

(1998) Two-dimensional electrophoretic analysis of mixed lineage kinase 2 N-terminal domain binding proteins. Electrophoresis. 19: 809-817.

38. Zugaro, L.M., Reid, G.E., Ji, H., Murphy, A., Burgess, A.W. and Simpson, R.J. (1998)

Characterisation of two-dimensional gel resolved rat brain stathmin isoforms by matrix-assisted laser desorption/ionisation and electrospray ionisation-ion trap mass spectrometry. Electrophoresis. 19: 867-876.

37. Chen. Y.C., Reid, G.E., Simpson, R.J. and Truscott, R.J.W. (1998) Molecular evidence for the

involvement of alpha crystallin in the colouration/crosslinking of crystallins in age-related nuclear cataract. Exp. Eye Res. 65: 835-840.

36. Ji, H., Reid, G.E., Moritz, R.L., Eddes, J.S., Burgess, A.W., and Simpson, R.J (1997) A two-

dimensional gel database of human colon carcinoma proteins. Electrophoresis. 18: 605-613.

35. Ji, H., Moritz, R.L., Reid, G.E., Ritter, G., Catimel, B., Nice, E., Heath, J.K., White, S.J., Welt, S., Old, L.J., Burgess, A.W., and Simpson, R.J. (1997) Electrophoretic analysis of the novel antigen for the gastrointestinal-specific monoclonal antibody, A33. Electrophoresis. 18: 614-621.

34. Rasmussen, R.K., Ji, H., Eddes, J.S., Moritz, R.L., Reid, G.E., Simpson, R.J., and Dorow, D.S.

(1997) Two-dimensional electrophoretic analysis of human breast carcinoma proteins: Mapping of proteins that bind to the SH3 domain of mixed lineage kinase MLK2. Electrophoresis. 18: 588-598.

33. Tong, D., Moritz, R.L., Eddes, J.S., Reid, G.E., Rasmussen, R.K., Dorow, D.S., and Simpson,

R.J. (1997) Fabrication of stable packed capillary reversed-phase columns for protein structural analysis. J. Prot. Chem. 16: 425-431.

32. Hammacher, A., Reid, G.E., Moritz, R.L., and Simpson, R.J. (1997) Identification and

characterisation of recombinant murine interleukin-6 with a C-terminal peptapeptide extension using capillary-MS and Edman degradation. Biomed. Chrom. 11: 337-342.

31. King, C., Simpson, R.J., Moritz, R.L., Reid, G., Thompson, P.J., and Stewart, G.A. (1996) The

isolation and characterisation of a novel collagenolytic serine protease allergen (Der p9) from the dust mite dermatophagoides pteronyssinus. J. Allergy Clin. Immunol. 98: 739-747.

30. Hodder, A.N., Crewther, P.E., Matthew, M.L., Reid, G.E., Moritz, R.L., Simpson, R.J., and

Anders, R.F. (1996) The disulfide structure of Plasmodium apical membrane antigen-1. J. Biol. Chem. 271: 29446-29452.

29. Moritz, R.L., Eddes, J.S., Reid, G.E., and Simpson, R.J. (1995) S-pyridylethylation of intact

polyacrylamide gels and in-situ digestion of electrophoretically-separated proteins: A rapid mass spectrometric method for identifying cysteine-containing peptides. Electrophoresis. 17: 907-917.

28. Simpson, R.J., Zhang, J-G., Dorow, D.S., Reid, G.E., Moritz, R.L., and Tu, G-F. (1995)

Identification of truncated E.Coli expressed proteins with a novel C-terminal 10Sa RNA decapeptide extension. In: Techniques in Protein Chemistry VII. Academic Press. pp 481-492.

27. Heath, R.L., Simpson, R.J., Reid, G.E., Lim, G., Barton, P.A., and Anderson, M.A. (1995)

Processing of a multidomain precursor to produce five homologous proteinase inhibitors in the stigma of Nicotiana Alata. Eur. J. Biochem. 230: 250-257.

26. Reid, G.E., Ji, H., Eddes, J.S., Moritz, R.L and Simpson, R.J. (1995) Non- reducing two

dimensional polyacrylamide gel electrophoretic analysis of human colonic proteins. Electrophoresis. 16: 1120-1130.

25. Simpson, R.J., Reid, G.E., Moritz, R.L., and Ward, L.D. (1995) Isolation of proteins and peptides

for sequence analysis. In: Encyclopedia of Molecular Biology: Fundamentals and Applications. Blackwell Scientific Publications. pp 173-185.

24. Inglis, A.S, Reid, G.E. and Simpson, R.J. (1995) Chemical techniques employed for the primary

structure analysis of protein and peptides, In: Interface between Chemistry and Biochemistry. (Jollés, P. and Jörnvall, H., Eds.). Birkhäuser Verlag A.G., Basel. pp 141-171.

23. Tu, G-F., Reid, G.E., Zhang, J-G., Moritz, R.L., and Simpson, R.J. (1995) C-terminal extention of

truncated recombinant proteins in E.coli with a 10Sa RNA decapeptide. J. Biol. Chem. 270: 9322-9326.

22. Mulhern, T.D., Howlett, G.J., Reid, G.E., Simpson, R.J., McColl, D.J., Anders, R.F. and Norton,

R.S. (1995) Solution structure of a polypeptide containing four heptad repeat units from a metozoite surface antigen of Plasmodium falciparum. Biochemistry. 34: 3479-3491.

21. Moritz, R.L., Eddes, J., Ji, H., Reid, G.E. and Simpson, R.J. (1994). High- speed

chromatographic separation of proteins and peptides: Application to rapid peptide mapping of in-gel digested proteins. J.Prot.Chem. 13: 486-487.

20. Moritz, R.L., Eddes, J., Ji, H., Reid, G.E., and Simpson, R.J. (1994) Rapid separation of proteins

and peptides using conventional silica-based supports: Identification of 2-D gel proteins following in-gel proteolysis. In: Techniques in Protein Chemistry VI. Academic Press. pp 319-321.

19. Moritz, R.L., Reid, G.E., Ward, L.D., and Simpson, R.J. (1994) Capillary HPLC: A method for

protein isolation and peptide mapping. In: Methods: A Companion to Methods in Enzymology. Academic Press. 6: 213-226.

18. Ji, H., Whitehead, R.H., Reid, G.E., Moritz, R.L., Ward, L.D., and Simpson, R.J. (1994) Two-

dimensional electrophoretic analysis of proteins expressed by normal and cancerous human crypts: Application of mass spectrometry to peptide-mass fingerprinting. Electrophoresis. 15: 391-405.

17. Zhang, J.G., Reid, G.E., Moritz, R.L., Ward, L.D., and Simpson, R.J. (1993) Specific covalent

modification of the tryptophan residues in murine interleukin-6: Effect on biological activity and confirmational stability. Eur. J. Biochem. 217: 53-59.

16. Lackmann, M., Cornish, C.J., Simpson, R.J., Moritz, R.L., Reid, G.E., and Geczy, C.L. (1992)

Isolation and structural analysis of a murine chemotactic protein CP-10. In: Pathophysiology and Pharmacology of Cytokines. Biomedical Press. pp 273-279.

15. Ramsay, R.G., Thompson, M.A., Hayman, J.A., Reid, G., Gonda, T.J., and Whitehead, R.H.

(1992) Myb expression is higher in Malignant human carcinoma and premalignant adenomatous polyps than in normal mucosa. Cell Growth and Differentiation. 3: 723-730.

14. Zhang, J.G., Moritz, R.L., Reid, G.E., Ward, L.D., and Simpson, R.J. (1992) Purification and

characterization of a recombinant murine interleukin-6: Isolation of N- and C- terminally truncated forms. Eur. J. Biochem. 207: 903-913.

13. Reid, G.E., and Simpson, R.J. (1992) Automated solid phase peptide synthesis: Use of 2-(1H-

Benzotriazol-yl)1,1,3,3,-tetramethyluronium tetrafluoroborate (TBTU) for coupling of Boc amino acids. Anal. Biochem. 200: 301-309.

12. Reid, G.E., and Simpson, R.J. (1992) Use of 2-(1H-Benzotriazol-yl)1,1,3,3,-tetrametyluronium

tetrafluoroborate (TBTU) in rapid coupling of Boc-amino acids: Adaptation to automated solid phase-peptide synthesis. In: Peptides: Chemistry and Biology. Smith, J.A. and Rivier, J.E. (Eds). Escom, Leidon. pp 647-648.

11. Norton, R.S., Macek, P., Reid, G.E., and Simpson, R.J. (1992) Relationship between the

cytolysins tenebrosin-C from Actinia tenebrosa and equinatoxin II from Actinia equina. Toxicon. 30: 13-23.

10. Layton, J.E., Morstyn, G., Fabri, L.J., Reid, G.E., Burgess, A.W., Simpson, R.J., and Nice, E.C.

(1991) Identification of a functional domain of Human Granulocyte Colony Stimulating Factor using neutralizing monoclonal antibodies. J. Biol. Chem. 266: 23815-23823.

9. Inglis, A.S., Moritz, R.L., Begg, G.S., Reid, G.E., Simpson, R.J., Graffunder, H., Matschull, L. and

Wittmann-Liebold, B. (1991) C-terminal sequence analysis. In: Methods in Protein Sequence Analysis. H. Jornvall (Ed). Birkhauser Verlag AG, Switzerland. pp 23-34.

8. Simpson, R.J., Moritz, R.L., Reid, G.E. and Ward, L.D. (1991) Current strategies for microscale purification of protein and peptides for sequence analysis. In: Methods in Protein Sequence Analysis. H. Jornvall (ed). Birkhauser Verlag AG, Switzerland. pp 67-77.

7. Ward, L.D., Reid, G.E., Moritz, R.L. and Simpson, R.J. (1990) Strategies for internal amino acid

sequence analysis of proteins separated by polyacrylamide gel electrophoresis. J. Chromatogr. 519: 199-216.

6. Simpson, R.J., Reid, G.E., Moritz, R.L., Morton, C. and Norton, R.S. (1990) Complete amino acid

sequence of tenebrosin-C, a cardiac stimulatory and haemolytic protein from the sea anemone Actinia tenebrosa. Eur. J. Biochem. 190: 319-328.

5. Jahnen, W., Ward, L.D., Reid, G.E., Moritz, R.L. and Simpson, R.J. (1990) Internal amino acid

sequencing of proteins by in situ cyanogen bromide cleavage in polyacrylamide gels. Biochem. Biophys. Res. Comm. 166: 139-145.

4. Ward, L.D., Reid, G.E., Moritz, R.L. and Simpson, R.J. (1990) Peptide mapping and internal

sequencing of proteins from acrylamide gels. In: Current Research in Protein Chemistry: Techniques, Structure and Function. J.J. Villafranca (ed). Academic Press. pp. 179-190.

3. Ward, L.D., Mortiz, R.L., Reid, G.E. and Simpson, R.J. (1989) 2D gel electrophoresis of proteins

from LIM1863 cells: identification of proteins by protein sequencing. In: Protein Engineeering. M. Ikehara (ed) Japan Scientific Societies Press,Tokyo and Springer-Verlag, Berlin. pp. 295-297.

2. Simpson, R.J., Ward, L.D., Reid, G.E., Batterham, M.P. and Moritz, R.L. (1989) Peptide mapping

and internal sequencing of proteins electroblotted from two-dimensional gels onto polyvinylidene difluoride membranes. A chromatographic procedure for separating proteins from detergents. J. Chromatogr. 476: 345-361.

1. Simpson, R.J., Begg, G.S., Reid, G.E., Rubira M.R., Ward L.D., and Moritz R.L. (1989) Application

of Microbore HPLC for purifying proteins and peptides for microsequence analysis. In: Methods in Protein Sequence Analysis. (ed. B. Wittmann-Liebold) Springer-Verlag, Berlin, pp. 256-263.

PATENTS AND DISCLOSURES: Granted: 1. Foote, Simon; Elso, Colleen; Simpson, Richard; Reid, Gavin. Nucleic acid diagnostics based on

base specific cleavage and MALDI-TOF mass spectrometry. US Patent 6,994,960. 2. McLuckey, Scott A.; Reid, Gavin E.; Wells, James Mitchell. Method of selectively inhibiting

reaction between ions. US Patent 7,355,169. 3. Hansen, Stuart C., Li, Gangquiang and Reid, Gavin E. Segmented rod multipole as ion

processing cell. US Patent 7,557,343. PCT: 1. Reid, Gavin E.; Hogan, Jason M.; Mcluckey, Scott A. Protein identification from protein product

ion spectra. PCT Int. Appl. (2003), WO 2003/098190 2. McLuckey, Scott A., Reid, Gavin E. and Wells, James Mitchell. Method of selectively inhibiting

reaction between ions for use in a three-dimensional quadrupole ion trap. PCT Int. Appl. (2003), WO 2003/017319

3. Reid, Gavin; Simpson, Richard; O'Hair, Richard. Method for analyzing amino acids, peptides and proteins. PCT Int. Appl. (2004), WO 2004/046731

4. Neu, Henrik; Olsen, Lee; Reid, Gavin E.; Roberts, Kade D. Substituted acetophenone as ms reagents for identification of proteins or quantitative determination of proteins. PCT Int. Appl. (2005), WO 2005/110955

5. Reid, Gavin E.; Roberts, Kade D.; Neu, Henrik; Liminga, Maria. Carbon-13 labeled phenacylamide compounds for us as fixed charge reagents. PCT Int. Appl. (2006), WO 2006/019354

6. Reid, Gavin E.; Roberts, Kade D. Modular isotope labelled mass spectrometry reagents and methods for quantitation of amino acids, peptides and proteins. PCT Int. Appl. (2006), WO 2006/023398

7. Spence, Dana and Reid, Gavin Edmund. Erythrocyte ATP-release modulators, compositions, and use for the treatment of glucose processing or vascular disorders. PCT Int. Appl. (2008) WO 2008/118387

8. Dantus, Marcos and Reid, Gavin E. Ultrafast Laser System for Biological Mass Spectrometry. PCT Int. Appl. (2010). US 2010/0123075

CONFERENCE PRESENTATIONS: 2012 Keynote Lecture 19th International Mass Spectrometry Conference, Kyoto, Japan

(Scheduled, September) Speaker 24th ASMS Sanibel Conference, St. Pete Beach, FL, USA.

2011 Invited Speaker 38th Conference of the Federation of Analytical Chemistry and Spectroscopy Societies. Reno, NV, USA.

Featured oral American Society for Mass Spectrometry 2011 Asilomar Conference. presentation Invited Speaker Gordon Research Conference Conference on Biological Molecules in the

Gas Phase. Andover, NH, USA. Invited Speaker Latin American Mass Spectrometry workshop. Universidad Industrial de

Santander. Bucaramanga, Columbia Speaker 59th Conference of the American Society for Mass Spectrometry.

Denver, CO, USA. Invited Speaker 16th Annual Proteomics Symposium. Lorne, Victoria, Australia Keynote Lecture 23rd Conference of the Australian and New Zealand Society for Mass

Spectrometry. Freemantle, Western Australia Invited Speaker 23rd ASMS Sanibel Conference, St. Pete Beach, FL, USA.

2010 Speaker 23rd Annual Lake Louise Tandem Mass Spectrometry Workshop. Alberta, Canada

Panel Member US-China Analytical workshop. Purdue University, West Lafayette, IN. USA

Speaker Peptide Fragmentation Interest Group workshop. 58th Conference of the American Society for Mass Spectrometry. Salt Lake City, UT. USA.

Invited Speaker Pittsburgh Conference. Orlando, FL. USA. 2009 Invited Speaker 36th Conference of the Federation of Analytical Chemistry and

Spectroscopy Societies. Louisville, KY. USA. Invited ‘State of Human Proteome Organization. 8th Annual World Congress. the Art Lecture’ Toronto, Canada. Speaker 57th Conference of the American Society for Mass Spectrometry.

Philadelphia, PA. USA. Invited Speaker. Peptide Fragmentation Interest Group workshop. 57th Conference

of the American Society for Mass Spectrometry. Philadelphia, PA. USA. Invited Speaker ACHEMA 2009 Conference. Frankfurt, Germany. Keynote Lecture 14th Annual Proteomics Symposium. Lorne, Victoria, Australia.

Speaker 14th Annual Proteomics Symposium. Lorne, Victoria, Australia. Invited Speaker LabAutomation 2009, Palm Springs, CA. USA.

2008 Keynote Lecture 21st Annual Lake Louise Tandem Mass Spectrometry Workshop. Alberta, Canada.

Speaker Midwestern Universities Analytical Chemistry Conference.

Bloomington, IN. USA. Invited Speaker 35th Conference of the Federation of Analytical Chemistry and

Spectroscopy Societies. Reno, NV, USA. Speaker 56th Conference of the American Society for Mass Spectrometry.

Denver, CO, USA. 2007 Invited Speaker The Michigan Society of Toxicology. Fall 2007 meeting. East

Lansing, MI, USA. Invited ‘State of Human Proteome Organization. 6th Annual World Congress. the Art Lecture’ Seoul, Korea. Invited Speaker. 55th Conference of the American Society for Mass Spectrometry.

Indianapolis, IN, USA. 2006 Invited Speaker. American Society for Mass Spectrometry 2006 Fall Workshop.

San Diego, CA. USA. Oral Presentation 54th Conference of the American Society for Mass Spectrometry. Seattle,

WA, USA. Invited Speaker. Ion trap Interest Group workshop. 54th Conference of the American

Society for Mass Spectrometry. Seattle, WA. USA. Invited Speaker. Michigan Mass Spectrometry Discussion Group. East Lansing, MI. USA.

2005 Invited Speaker. The Association of Analytical Chemistry. ANACHEM Symposium. Livonia, MI. USA.

Invited Speaker. 32nd Conference of the Federation of Analytical Chemistry and Spectroscopy Societies. Quebec, Canada

Invited Speaker. Ion trap Interest Group workshop. 53rd Conference of the American Society for Mass Spectrometry. San Antonio, TX. USA.

Poster Presentation. 53rd Conference of the American Society for Mass Spectrometry. San Antonio, TX. USA.

2004 Invited Speaker 31st Conference of the Federation of Analytical Chemistry and Spectroscopy Societies. Portland, OR, USA.

2003 Oral Presentation 51st Conference of the American Society for Mass Spectrometry. Montreal, Canada.

Keynote Lecture 19th Conference of the Australian and New Zealand Society for Mass Spectrometry. Lorne, Victoria, Australia.

2002 Invited Speaker Human Proteome Organization. 1st World Congress. Versailles, France Oral Presentation 50th Conference of the American Society for Mass Spectrometry Orlando,

FL, USA 2001 Invited Speaker American Chemical Society. Regional Conference. Grand Rapids, MI,

USA. Poster Presentation 49th Conference of the American Society for Mass Spectrometry.

Chicago, IL, USA. Oral Presentation 6th Annual Proteomics Workshop. Lorne, Victoria, Australia. 1999 Oral Presentation. 47th Conference of the American Society for Mass Spectrometry. Dallas,

TX, USA. Oral Presentation 17th Conference of the Australian and New Zealand Society for Mass

Spectrometry. Thredbo NSW. Oral Presentation Lorne ‘99. Specialist Protein Analysis Workshop. Lorne, Victoria,

Australia. Poster Presentation 24th Lorne Conf. on Protein Structure and Function. Lorne, Victoria,

Australia.. 1998 Oral Presentation 46th American Society for Mass Spectrometry Conference. Orlando, FL,

USA. Oral Presentation Specialist Protein Analysis Workshop. Lorne, Victoria, Australia. Poster Presentation 23rd Lorne Conf. on Protein Structure and Function. Lorne, Victoria,

Australia. 1997 Oral Presentation 3rd Australian Molecular Modelling Workshop, Melbourne, Victoria,

Australia. Oral Presentation Specialist Protein Analysis Workshop. Lorne, Victoria, Australia.

Poster Presentation 22nd Lorne Conf. on Protein Structure and Function. Lorne, Victoria, Australia.

1996 Poster Presentation ABRF '96. Biomolecular Techniques. San Fransisco, CA. USA. Oral Presentation Specialist Protein Analysis Workshop. Lorne, Victoria, Australia. Poster Presentation 21st Lorne Conf. on Protein Structure and Function. Lorne, Victoria,

Australia. Oral Presentation Chromatography ‘96: Separation Sciences. Sydney, NSW. 1995 Poster Presentation 20th Lorne Conf. on Protein Structure and Function. Lorne, Victoria,

Australia. 1994 Poster Presentation 3rd Int. Symposium on Mass Spectrometry in the Health and Life

Sciences. San Francisco, CA. USA. Poster Presentation 19th Lorne Conf. on Protein Structure and Function. Lorne, Victoria,

Australia. 1993 Poster Presentation 18th Lorne Conf. on Protein Structure and Function. Lorne, Victoria,

Australia. 1992 Poster Presentation 17th Lorne Conf. on Protein Structure and Function. Lorne, Victoria,

Australia. 1991 Poster Presentation 16th Lorne Conf. on Protein Structure and Function. Lorne, Victoria,

Australia. 1990 Poster Presentation 15th Lorne Conf. on Protein Structure and Function. Lorne, Victoria,

Australia. INVITED SEMINARS / OTHER PRESENTATIONS: 2012 Seminar Department of Chemistry. University of Akron, OH

Seminar Department of Chemistry. University of North Texas. TX 2011 Seminar Department of Chemistry. University of Toronto, Canada

Department of Chemistry. York University, Canada Department of Chemistry. Calvin College, MI

Seminar Biomolecular Science and Engineering Program, University of California Santa Barbara, CA

Colloquium Department of Chemistry. Ohio University, OH. Seminar Department of Chemistry. University of Wollongong, Australia. 2009 Seminar Department of Chemistry. Indiana University. West Lafayette, IN. Seminar Department of Chemistry. Purdue University. West Lafayette, IN. Seminar Department of Chemistry.University of Western Ontario, London, ON, Canada Colloquium Department of Chemistry. University of Nebraska, Lincoln, NE

Seminar Department of Chemistry. University of California, Davis, CA Seminar Department of Chemistry. Wayne State University, MI.

Colloquium Department of Biochemistry and Molecular Biology. Michigan State University, MI 2008 Colloquium Department of Chemistry. Michigan State University, MI Seminar Department of Chemistry. St. Louis University. MO Seminar Institute for Systems Biology. Seattle, WA Colloquium Department of Chemistry. Northern Illinois University, IL Seminar Department of Chemistry. University of Massachusetts-Amherst MA Seminar Department of Chemistry. University of Michigan. MI Seminar Department of Chemistry. Texas A&M. TX 2007 Seminar Bio21 Institute. University of Melbourne. Vic. Australia.

Seminar Frontiers in Science Weekend Workshop series for Secondary Science Teachers, MSU

Seminar Analytical Sciences Division. Dow Chemical. Midland, MI Seminar Department of Chemistry. The College of William and Mary, VA. Seminar Department of Chemistry. Oakland University, MI 2005 Seminar Department of Biochemistry. Yonsei University, South Korea. Seminar Department of Chemistry. Wayne State University, MI. 2004 Seminar Quantitative Biology and Modeling Initiative. Dept. of Biochemistry

Michigan State University, MI. Seminar Department of Pharmacology. University of Melbourne. Vic. Australia. Seminar Department of Biochemistry. Monash University. Vic. Australia. Seminar Department of Chemistry. University of Wisconsin, WI. Seminar Dept. Chemistry. Michigan State University, MI. 2003 Seminar Austin Research Institute, Vic. Australia. 2002 Speaker RACI Spring Peptide Users Group Symposium. Vic. Australia. Speaker 2002 ANZSMS Annual General Meeting. Vic. Australia. Seminar Ludwig Institute for Cancer Research. Vic. Australia. Seminar Department of Chemistry. University of Wollongong. NSW. Australia. 2001 Speaker Deans Advisory Council. Faculty of Science. Purdue University. IN. 2000 Seminar School of Chemistry. University of Melbourne. Vic. Australia. 1997 Speaker RACI Industry Sponsored Student Presentation, School of Chemistry, University

of Melbourne, Vic. Australia. 1994 Speaker 2-D Electrophoresis Symposium. Macquarie University, NSW. Australia.