Curriculum Vitae Dr. Claus-Dieter Kuhn Personal data: Name: Dr. Claus-Dieter Kuhn Date of birth: July 19, 1978 Nationality: German Family status: married, 2 children CURRENT POSITION ________________________________________________________________________________ Since 09/2014 Junior Research Group Leader within the Elite Network of Bavaria at the

University of Bayreuth, URL: www.kuhnlab.uni-bayreuth.de EDUCATION ________________________________________________________________________________ 10/2003 - 02/2008 PhD Student at the Gene Center of the Ludwig-Maximilians-University in

Munich, in the Group of Prof. Dr. Patrick Cramer 08/2002 - 07/2003 Master of Science with a Major in Chemistry, Department of Biochemistry and

Biophysics, Stockholm University, Sweden 10/1999 - 07/2002 Undergraduate Studies in Biochemistry at the University of Regensburg PROFESSIONAL CAREER ________________________________________________________________________________ 03/2010 - 07/2014 Postdoctoral Fellow at Cold Spring Harbor Laboratory, NY, USA, in the

Group of Prof. Dr. Leemor Joshua-Tor 10/2009 - 02/2010 X-ray crystallographer with Proteros Biostructures, Martinsried 03/2008 - 09/2009 Postdoctoral Fellow at Cold Spring Harbor Laboratory, NY, USA, in the

Group of Prof. Dr. Leemor Joshua-Tor SCHOLARSHIPS, AWARDS AND GRANTS ________________________________________________________________________________ 2018 Grant from the Oberfrankenstiftung for our work on "The impact of Neuronal enhancer RNAs on Neuronal plasticity and Neurogenesis" 2017 DFG Grant as part of the Priority Program 1738 (Emerging roles of non- coding RNAs in nervous system development, plasticity and disease)

2016 Recipient of the Paul Ehrlich and Ludwig Darmstaedter Prize for Young Researchers (together with the Heinz-Maier Leibniz Prize the highest prize for Biomedical Research in Germany)

2016 DFG Grant for our work on the "Role of neuronal eRNAs in transcription activation"

2014 Junior Group Leader within the Elite Network of Bavaria at the University of Bayreuth

2008 - 2012 Postdoctoral Fellowship from the Jane Coffin Childs Memorial Fund for Medical Research, Yale University Medical School, Yale, USA

2005 - 2008 Member of the Elite Network of Bavaria Graduate Programs ‘Protein Dynamics in Health and Disease’ and ‘Nano-Biotechnology’

2008 Publication Award from the Center for Nanoscience, Ludwig-Maximilians-University Munich

2007 Römerprize from the Department of Chemistry and Biochemistry of the Ludwig-Maximilians-University Munich

2004 - 2006 Kekulé Scholarship from the Fund of German Chemical Industry (FCI) 1999 - 2003 Scholarship for highly gifted students from the Wilhelm-Narr-Foundation in

Kirchheim/Teck PUBLICATIONS ________________________________________________________________________________ Original articles 1. Iana V. Kim, Elisabeth M. Duncan, Eric J. Ross, Vladyslava Gorbovytska, Stephanie Nowotarski,

Sarah A. Elliott, Alejandro Sánchez Alvarado and Claus-D. Kuhn (2019). Planarians recruit piRNAs for mRNA turnover in adult stem cells. Genes & Development 33, 1575-1590

2. Iana V. Kim, Eric J. Ross, Sascha Dietrich, Kristina Döring, Alejandro Sánchez Alvarado and Claus-D. Kuhn (2019). Efficient ribodepletion for RNA sequencing in planarians. BMC Genomics, in press.

3. Dirk Kostrewa*, Claus-D. Kuhn*, Christoph Engel, and Patrick Cramer (2015). An alternative RNA polymerase I structure reveals a dimer hinge. Acta Crys. Section D 71, 1850-1855. (* contributed equally)

4. Claus-D. Kuhn, Jeremy E. Wilusz, Yuxuan Zheng, Peter A. Beal, and Leemor Joshua-Tor (2015). On-Enzyme Refolding Permits Small RNA and tRNA Surveillance by the CCA-Adding Enzyme. Cell 160, 1-15.

5. Jeremy E. Wilusz, Courtney K. JnBaptiste, Laura Y. Lu, Claus-D. Kuhn, Leemor Joshua-Tor, and Phillip A. Sharp (2012). A triple helix stabilizes the 3' ends of long noncoding RNAs that lack poly(A) tails. Genes and Development 26, 2392-2407.

6. Elad Elkayam, Claus-D. Kuhn, Ante Tocilj, Astrid D. Haase, Emily M. Greene, Gregory J. Hannon, and Leemor Joshua-Tor (2012). The Structure of Human Argonaute-2 in Complex with miR-20a. Cell 150, 100-110.

7. Sebastian R. Geiger, Claus-D. Kuhn, Christoph Leidig, Jörg Renkawitz, and Patrick Cramer (2008). Crystallization of the RNA polymerase I subcomplex A14/43 by iterative prediction, probing, and removal of multiple flexible regions. Acta Crys. Section F 64, 413-418.

8. Jochen Gerber, Alarich Reiter, Robert Steinbauer, Steffen Jakob, Claus-D. Kuhn, Patrick Cramer, Joachim Griesenbeck, Philipp Milkereit, and Herbert Tschochner (2008). Site specific phosphorylation of yeast RNA polymerase I. Nucleic Acids Res. 36, 793-802.

9. Claus-D. Kuhn, Sebastian R. Geiger, Sonja Baumli, Marco Gartmann, Jochen Gerber, Stefan Jennebach, Thorsten Mielke, Herbert Tschochner, Roland Beckmann, and Patrick Cramer (2007). Functional architecture of RNA Polymerase I. Cell 131, 1260-1272.

10. Shashi Bhushan, Claus-D. Kuhn, Anna-Karin Berglund, Christian Roth, and Elzbieta Glaser (2006). The role of the N-terminal domain of chloroplast targeting peptides in organellar protein import and miss-sorting. FEBS Letters 580, 3966-7.

Articles submitted or in revision 11. Felix Klatt, Alexander Leitner, Iana V. Kim, Xuan-Hung Ho, Elisabeth V. Schneider, Franziska

Langhammer, Robin Weinmann, Melanie R. Müller, Robert Huber, Gunter Meister and Claus-D. Kuhn (2019). MED12 wraps around CDK8 and utilizes an activation helix to stimulate its kinase activity. PNAS, in revision.

12. Longhua Guo, Fengli Guo, Shasha Zhang, Kexi Yi, Melainia McClain, Claus-D. Kuhn, Tari Parmely and Alejandro Sánchez Alvarado (2019). Subcellular analyses of planarian meiosis implicate a novel, double-membraned vesiculation process in nuclear envelope breakdown. Molecular Biology of The Cell, in revision. (bioRxiv doi: 10.1101/620609)

Review articles 13. Iana Kim, Vladyslava Gorbovytska and Claus-D. Kuhn (2017). Wie RNA die Genregulation

beeinflusst. Biospektrum 23 (5), 513–515

14. Claus-D. Kuhn (2016). RNA flexibility governs tRNA function. BioEssays 38 (5), 465-73.

15. Claus-D. Kuhn and Leemor Joshua-Tor (2013). Eukaryotic Argonautes come into focus. Trends in Biochemical Sciences 38, 263-271.

16. Patrick Cramer, Karim J. Armache, Sonja Baumli, Stefan Benkert, Florian Brueckner, Claudia Buchen, Gerke E. Damsma, Stefan Dengl, Sebastian R. Geiger, Anja J. Jasiak, Anass Jawhari, Stefan Jennebach, Tomislav Kamenski, Hubert Kettenberger, Claus-D. Kuhn, Elisabeth Lehmann, Kristin Leike, Jasmin Sydow, and Alessandro Vannini (2008). Structure of Eukaryotic RNA Polymerases. Annual Review of Biophysics 37, 337-352.

REVIEWING ACTIVITY ________________________________________________________________________________ EMBO Journal, eLIFE, RNA, German Research Foundation (DFG), Wellcome Trust