Curriculum Vitae and Bibliography Roshini S. … University, India - BSc, Chemistry and Biology 1983 - 1986 Bangalore University, India - MSc, Microbiology 1986 - 1988 National Institute

Curriculum Vitae and Bibliography Roshini S. Abraham, PhD

Personal InformationPlace of Birth:

Citizenship:

Work Address: Mayo Clinic200 First Street SWRochester, MN 55905507-266-9292

Email Address: [email protected]

Present Academic Rank and Position Director - Cellular & Molecular Immunology Laboratory, Division of ClinicalBiochemistry & Immunology, Department of Laboratory Medicine and Pathology,Mayo Clinic, Rochester, Minnesota

07/2005 - Present

Master's Faculty Privileges in Biochemistry & Molecular Biology - MayoGraduate School, Mayo Clinic College of Medicine

03/2006 - Present

Consultant - Division of Clinical Biochemistry & Immunology, Department ofLaboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota

06/2007 - Present

Consultant (Joint Appointment) - Division of Allergic Diseases, Department ofInternal Medicine, Mayo Clinic, Rochester, Minnesota

06/2007 - Present

Director - Jeffrey Modell Foundation for Primary Immunodeficiencies Diagnosticand Research Center at the Mayo Clinic, Rochester, Minnesota

09/2009 - Present

Professor of Laboratory Medicine & Pathology - Mayo Clinic College ofMedicine

09/2014 - Present

Professor of Medicine - Mayo Clinic College of Medicine 09/2014 - Present

Education Jabalpur University, India - BSc, Chemistry and Biology 1983 - 1986

Bangalore University, India - MSc, Microbiology 1986 - 1988

National Institute of Immunology, New Delhi, India - PhD, Immunology 1991 - 1996

Department of Immunology, Mayo Clinic - Post Doctoral Fellowship, Immunology 1996 - 2000

Division of Clinical Biochemistry & Immunology, Department of LaboratoryMedicine and Pathology, Mayo Clinic - Clinical Post Doctoral Fellowship, ClinicalBiochemistry and Immunology

2000 - 2002

Additional Education

Flow CytometryBD BioSciences Advanced Course on Flow CytometrySan Diego, California

08/2005

NIH Grant Writing WorkshopNIH WorkshopTucson, Arizona

08/2002

RE-AIMS Sep 29, 2014

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Biacore BasicsOperation of the Biacore instrument and data analysis of surface plasmonresonance experimentsBIABASICSNew Jersey

07/2002

CertificationsBoard Certifications

American Board of Medical Laboratory Immunology (ABMLI)

Diplomate 2007 - 2016

New York State Department of Health

Certificate of Qualification (COQ) in Cellular and Diagnostic Immunology, GeneticTesting for Primary Immunodeficiencies

2005 - 2015

Honors/Awards Junior Research Fellowship, Department of Biotechnology - Government ofIndia

07/1991 - 06/1993

Senior Research Fellowship, Department of Biotechnology - Government ofIndia

07/1993 - 09/1996

Postdoctoral Fellowship - Juvenile Diabetes Foundation International 07/1998 - 06/2000

George Grannis Award for Excellence in Research and ScientificPublication - National Academy of Clinical Biochemistry

2003

Award for Outstanding Scientific Achievements by a Young Investigator -American Association for Clinical Chemistry

2006

Doctorate Award - Association of Medical Laboratory Immunologists 2006

Travel scholarship for the IUIS/WHO meeting on PrimaryImmunodeficiencies - US Immunodeficiency Network, Jackson Hole, Wyoming

2007

Fellow - American Academy of Allergy, Asthma and Immunology 2010 - Present

Previous Professional Positions and Major Appointments Project Associate - Department of Microbiology and Cell Biology, Indian Instituteof Science, Bangalore, India

1989 - 1991

Senior Associate Consultant - Division of Hematology, Department of InternalMedicine, Mayo Clinic, Rochester, Minnesota

07/2002 - 06/2005

Assistant Professor of Medicine - Mayo Clinic College of Medicine 10/2002 - 02/2009

Associate Faculty Privileges in Cell Biology/Genetics - Mayo GraduateSchool, Mayo Clinic College of Medicine

06/2004 - 03/2006

Senior Associate Consultant (Joint Appointment) - Division of AllergicDiseases, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota

07/2005 - 06/2007

Senior Associate Consultant - Division of Clinical Biochemistry & Immunology,Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester,Minnesota

07/2005 - 06/2007

Assistant Professor of Laboratory Medicine & Pathology - Mayo ClinicCollege of Medicine

01/2006 - 02/2009

Associate Professor of Laboratory Medicine & Pathology - Mayo ClinicCollege of Medicine

03/2009 - 08/2014

Associate Professor of Medicine - Mayo Clinic College of Medicine 03/2009 - 08/2014

Professional & Community Memberships, Societies and Services Professional Memberships & ServicesAmerican Association of Immunologists


Roshini S. Abraham, PhD Page 2 of 40

Member 10/1996 - Present

Sigma Xi, The Scientific Research Society

Mayo Foundation Chapter

Executive Committee Member 2006 - 2008

Member, Rochester, Minnesota 01/1997 - 2013

Association of Medical Laboratory Immunologists

Member 2005 - Present

Councilor 2008 - 2011

Scientific Program Organizer 2008 - 2014

Clinical Immunology Society

Member 01/2005 - Present

Elected Councilor 2013 - 2016

President-Elect 2016 - 2017

President 2017 - 2018

Past President 2018 - 2019

School on Diagnostic Assessment of Immune phenotyping and function inPrimary Immunodeficiencies

Program Chair, Baltimore, Maryland 04/2014

American Academy of Allergy, Asthma and Immunology


Basic & Clinical Immunology (BCI)Section/Primary ImmunodeficiencyDiseases Committee

Member 2008 - 2011

Primary Immunodeficiency Subcommittee Working Group on NewbornScreening for Severe Combined Immunodeficiency (SCID)

Member 2010 - 2012

American Board of Medical Laboratory Immunologists

Member 2011 - 2017

Board member

Examination Validation Committee

Member 2007 - 2010

Young Diplomates Committee

Member 2008 - 2010

Credentials Committee

Chairman 2012 - 2015

Member 2009 - 2012

Exam Development Committee

Member 2010 - 2013

European Society for Immunodeficiencies


Jeffrey Modell Foundation for Primary Immunodeficiencies

Director 09/2009 - Present

Director, Diagnostic and Research Center at the Mayo Clinic



Research Committee

Research Faculty Member, New York, New York 07/2013 - Present

International Society for Advancement of Cytometry


American Society of Transplantation


International Clinical Cytometry Society


Newborn Screening Translational Research Network (NBSTRN) and Region 4Stork International Database for Severe Combined Immunodeficiency

Co-Coordinator 2010 - Present

Clinical Laboratory Standards Institute (CLSI)

Document Development Committee - On Severe combinedImmunodeficiencies

Vice Chair 2011 - 2013

Journal Responsibilities Journal Editorial ResponsibilitiesJournal of Immunological Research

Special Issue Editor 2013 - 2014

Lead Guest Editor for special edition on Primary Immunodeficiencies

Journal Other ResponsibilitiesClinical Immunology

Reviewer 2007 - Present

Journal of Clinical Immunology


Annals of Allergy, Asthma & Immunology


Blood


American Journal of Transplantation


PLoS ONE


Journal of Immunological Methods


Journal of Pediatric Infectious Diseases


Human Immunology


Journal of Allergy and Clinical Immunology


Transplant Infectious Diseases




Educational ActivitiesCurriculum/Course Development

Humman Immunology/PathologyCourse on clinical immunology using a case-based approach to learn basicconcepts for 1st year medical students.Mayo Medical SchoolRochester, Minnesota

10/2013 - 02/2014

Teaching IntramuralAllergy/Immunology Fellows Quarterly Case Conference SeriesAllergy/Immunology Fellows (Quarterly Lecture Series on Clinical Immunology,Primary Immunodeficiencies and Transplant Immunology) - Clinical ImmunologyLectures - 1Mayo Clinic - RochesterRochester, Minnesota

03/2014

Allergy Immunology Case ConferenceTest Utilization: Improving the diagnostic testing for Primary ImmunodeficienciesMayo Clinic - RochesterRochester, Minnesota

02/2014

Case studies in Primary Immunodeficiencies-1Mayo Medical School

02/2014

Case studies in Primary Immunodeficiencies-2Mayo Medical School

02/2014

Core rotation in Cellular and Molecular Immunology for Clinical Chemistry FellowsMayo Graduate School Of Medicine

01/2014

Principles of clinical immunology diagnostic flow cytometry-1Mayo Medical School

01/2014

Principles of clinical immunology diagnostic flow cytometry-2Mayo Medical School

01/2014

Therapeutic Antibodies and Introduction to Primary ImmunodeficienciesMayo Medical School

01/2014

Clinical Pathology/Clinical Chemistry Seminars for Pathology Residents andClinical Chemistry FellowsMayo Graduate School of Medicine

12/2013

Flow cytometry for Primary ImmunodeficienciesWeb-based lectureInternational Clinical Cytometry Society

10/2013

Allergy/Immunology Fellows Quarterly Case Conference SeriesAllergy/Immunology Fellows (Quarterly Lecture Series on Clinical Immunology,Primary Immunodeficiencies and Transplant Immunology) - Clinical ImmunologyLectures - 1Mayo Clinic - RochesterRochester, Minnesota

09/2013

Laboratory rotation in Cellular and Molecular Immunology for ClinicalAllergy/Immunology FellowsMayo Graduate School of Medicine

09/2013

Medical Laboratory Science Program, Clinical Immunology CoursePrimary Immunodeficiencies Immunoproliferatiove disorders and transplantMayo School of Health SciencesRochester, Minnesota

09/2013




08/2013

Allergy/Immunology Fellows Quarterly Case Conference SeriesAllergy/Immunology Fellows (Quarterly Lecture Series on Clinical Immunology,Primary Immunodeficiencies and Transplant Immunology) - Clinical ImmunologyLectures - 1Mayo Clinic - RochesterRochester, Minnesota

06/2013


01/2013

Mayo Medical Laboratories Web-based s(Webinar) - Hot Topic Seminar seriesTwo-part seriesMayo Medical LaboratoriesRochester, Minnesota

01/2013

Clinical Biochemistry & Immunology, Lab Genetics Seminar SeriesHemophagocytic Lymphohistiocytosis: Not a Happy Meal!Mayo Clinic - RochesterRochester, Minnesota

12/2012

Clinical Laboratory Science Program, Clinical Immunology CoursePrimary Immunodeficiencies Immunoproliferatiove disorders and transplantMayo School of Health SciencesRochester, Minnesota

10/2012


09/2012

Immunology-B cell tutorial/Dep[artment of Immunology Graduate School ClassTwo lecture series every two yearsMayo Graduate School

04/2012

Flow cytometry for the diagnosis of Primary ImmunodeficienciesWeb-based lectureAssociation of Medical Lab Immunologists (AMLI) and American Association ofClinical Chemistry (AACC) joint educational conference

02/2012


01/2012

Allergy Immunology Case ConferenceUpdate on Common Variable Immunodeficiency (CV(D)Mayo Clinic - RochesterRochester, Minnesota

12/2011

Mayo DLMP Business Development Group PresentationFlow cytometry for the diagnosis of Primary ImmunodeficienciesMayo Clinic - RochesterRochester, Minnesota

12/2011


09/2011


09/2011



Clinical Laboratory Science Program, Clinical Immunology CoursePrimary Immunodeficiencies Immunoproliferatiove disorders and transplantMayo School of Health SciencesRochester, Minnesota

09/2011

Allergy/Immunology Journal Club SeriesSCID (Severe Combined Immunodeficiency) and Immunomodulatroy therapiesand Biological agentsMayo Clinic RochesterRochester, Minnesota

06/2011

Clinical Biochemistry & Immunology, Lab Genetics Seminar SeriesMayo Clinic - Rochester

05/2011

Pharmacology CourseTwo lectures on Immune monitoring and biological immunomodulatorsMayo Medical School

04/2011

Clinical Laboratory Science Program, Clinical Immunology CourseMayo School of Health Sciences

11/2010


09/2010

Immunological testing to enhance transplant practiceTransplant Grand Rounds, Mayo ClinicRochester, Minnesota

06/2010

New guidelines for interpretation of pneumococcal antibody testing and analysisof B cell subsets after pneumococcal vaccinationCollaborative seminar with Ospedale Pediatrico Bambino Gesu, Rome, ItalyRochester, Minnesota

05/2010

Allergy Immunology Case ConferenceMayo Clinic - Rochester

04/2010

Immunology-B cell tutorial/Dep[artment of Immunology Graduate School ClassTwo lecture series every two yearsMayo Graduate School

04/2010

Advances in Immunological Analysis for Cardiac TransplantationDivision of Cardiothoracic Transplantation Seminar series Mayo Clinic - Rochester

03/2010

Induction therapy in lung transplantation: relevance of immunological analysesDivision of Pulmonary Medicine Research seminar series Mayo Clinic - Rochester

03/2010

Advances in diagnostic approaches for Primary ImmunodeficienciesPediactric Grand Rounds, Mayo ClinicRochester, Minnesota

03/2010


01/2010

Update on Clinical Immunology Tests: Immune Competence and ImmuneReconstitutionClinical Immunology & Immunotherapeutics Program (CIIP)Mayo Clinic -Rochester

12/2009

Allergy/Immunology Case ConferenceDivision of Allergic DiseasesRochester, Minnesota

12/2009



Mayo Medical Laboratories Web-based seminar series (Webinar) - Hot TopicsMayo Medical LaboratoriesRochester, Minnesota

11/2009

Clinical Laboratory Science Program, Clinical Immunology CourseMayo School of Health Sciences

10/2009


09/2009


09/2009

Allergy/Immunology Case ConferenceDivision of Allergic DiseasesRochester, Minnesota

04/2009

Allergy/Immunology Journal ClubDivision of Allergic DiseasesRochester, Minnesota

04/2009


03/2009

Clinical Biochemistry and Immunology Division Seminar SeriesMayo Clinic - Rochester

02/2009


02/2009

Clinical Laboratory Science Program, Clinical Immunology CourseMayo Scool of Health Sciences

11/2008


09/2008


09/2008

Immunology Testing: Relevance to the Rheumatology PracticeRheumatology Research Forum, Mayo Clinic - Rochester

09/2008

Genetic Defects in CVID: Understanding the Immunological MechanismsMedical Genetics Research Forum

02/2008

Update on Immunology Testing for Clinical PracticeClinical Immunology & Immunotherapeutics Program (CIIP), Mayo Clinic -Rochester

12/2007

Hematologic Malignancies ForumMayo Clinic - Rochester

10/2007

Allergy/Immunology Case ConferenceMayo Clinic - Rochester

09/2007

Clinical Pathoology/Clinical Chemistry Seminars for Pathology Residents andClinical Chemistry FellowsOne to two lectures every two yearsMayo Graduate School of Medicine

09/2007



Core rotation in Cellular and Molecular Immunology for Clinical Chemistry FellowsMayo Graduate School of Medicine

09/2007


09/2007

New Opportunities for Clinical Diagnostic Testing in ImmunologyDermatology Hospital Practice Group, Mayo Clinic - Rochester

12/2006

Bone Marrow Transplant GroupMayo Clinic - Rochester

10/2006


09/2006


08/2006


05/2006

CME Lecture for Clinical Immunology Laboratory Allied Health StaffMayo Clinic - Rochester

03/2006

Bone Marrow Transplant GroupMayo Clinic - Rochester

02/2006

Division of Clinical Biochemistry and Immunology Seminar SeriesMayo Clinic - Rochester

02/2006


01/2006

Allergy/Hypersensitivity Reserach ForumMayo Clinic - Rochester

09/2005

Hematology Research GroupMayo Clinic - Rochester

06/2005

Cell Biology and Genetics CourseMayo Graduate School

02/2005

Dysproteinemia Research ForumMayo Clinic - Rochester

06/2002

Mentorship) (Hematology/Oncology Fellow)

Current Status:Second-year Pediatric Hematology-Oncology fellow Outcomes:First author on abstract presented at the Primary ImmunodeficiencyTreatment Consortium meeting on "Role of immunological and genetic testing inthe management of X-linked thrombocytopenia and Wiskott Aldrich syndrome".Mentoring for future career options as well as publishing the national presentationas a brief report.

02/2014 - 05/2014

(Pathology Resident) Description: is working in my lab as part of one of his electiverotations due to his interest in clinical immunology. He will be working ondeveloping a new clinical assay for NK cell function to measureantibody-dependent cellular cytotoxocity (ADCC). Current Status:1st year Pathology resident Outcomes:He is currently in pre-elective training and will complete the elective

01/2014



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by the end of June 2014.

(Neurology Resident) Description:May 2013 – Present, Dept. of Neurology, Mayo Clinic.

is working under my guidance on a research study (retrospective chartreview) to characterize autoimmune or other neurological complications inpatients with primary immunodeficiencies. Outcomes:Current mentee

05/2013 - 01/2014

(Consultant) Description:Primary mentor for 20% FTE, Department of Medicine ScholarlyTime Award. Outcomes:Consultant and Assistant Professor of Medicine, Division ofHematology, Department of Laboratory Medicine and Pathology, MayoClinic-Rochester.

04/2013 - 01/2014

(Allergy/Immunology Fellow) Description:Working under my supervision on a collaborative project withDuke University entitled, “Molecular andImmunological characterization of Common Variable Immunodeficiency”.Manuscript in preparation.

Identification of Novel BTK Gene Mutations in Patients with X-linkedAgammaglobulinemia and Female Carriers. Manuscript in preparation. ResearchMentor for 2nd year A/I fellowship project on longitudinal analysis of humoralimmunity to pneumococcal vaccination in healthy adults. 2 Abstracts submitted forpresentation in 2014. Additionally, 2 manuscripts are in preparation. Outcomes:2nd year Allergy Immunology Fellow

12/2012 - 06/2014

(Allergy/Immunology Fellow) Description:Worked under my guidance on a collaborative project with

, Dept of Immunology on clinical and immunological data correlation inpatients with Common Variable Immunodeficiency (CVID) with Flt-3 levels.Manuscript in preparation. Outcomes:Completed Allergy/Immunology Felllowship - June 2013; PrivatePractice Allergist/Immunologist.

11/2012 - 05/2013

(Undergraduate Student) Description:July –Sept 2012, 2 month visiting internship.

2 months in the Cellular and Molecular ImmunologyLaboratory during her graduate training (M.S. in Biotechnology). She learnt thefundamentals of cell culture, flow cytometry, clinical HIV diagnostic testing, genesequencing for inherited defects. Outcomes:Currently doing Ph.D. in Biology in

07/2012 - 09/2012

(Pathology Resident) Description: worked in my lab on a project to develop a new clinicaltest to assess NK cell cytotoxic function by flow cytometry. This project iscurrently in the validation phase and will be implemented as a panel of 2 newdiagnostic tests early 2015. Additionally, she also did a Lab Directorapprenticeship with me in the summer of 2013. Current Status:Chief Resident, Pathology Residency Program Outcomes: has been selected Chief Resident in the PathologyProgram and has gained admission to the Hematopathology Fellowship for 2015.

01/2012 - 01/2013

(Allergy/Immunology Fellow) Description:Dec 2011 to Feb 2012 – Present, Division of Allergic Diseases,Dept of Medicine, Mayo Clinic. worked with me on writing a review

01/2011 - 01/2012



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Outcomes:Completed Allergy/Immunology Fellowship - June 2013

(Molecular Genetic Pathology Fellow) Description:1 week elective rotation in Transplant Immunology Outcomes:Consultant Pathologist -

01/2011

(Clinical Chemistry Fellow) Description:Jan 2010 – present, Division of Clinical Biochemistry andImmunology, Dept of Laboratory Medicine and Pathology, Mayo Clinic,Rochester, MN has been working with me on a research projectevaluating the analytical variability in lymphocyte quantitation by flow cytometry.The mentoring relationship has included project design and elucidation ofconcepts, reviewing data and providing guidance and support on data analysis,abstract preparation and manuscript preparation. This is an ongoing interactionand research project. The following abstract has been submitted for the

, Abraham RS.

This study is alsopresently being written up for publication. Outcomes:Consultant, Division of Clinical Biochemistry & Immunology,Department of Laboratory Medicine and Pathology, Mayo Clinic-Rochester.

01/2010 - 06/2012

(Mayo Medical Student) Description:Working on a project to develop, validate and introduce a newfluorescent method for TB detection in sputum samples in a mission hospital in

. Outcomes:4th Year Medical student.

01/2010 - 01/2011

(Mayo Medical Student) Description:Working on a project to develop, validate and introduce a newfluorescent method for TB detection in sputum samples in a mission hospital in

. Outcomes:Resident - Internal Medicine - Mayo (Rochester)

01/2010 - 01/2011

(Visiting Clerk) Description:July – Oct 2009 (3 month temporary research traineeappointment), Cellular & Molecular Immunology Laboratory, Dept of LaboratoryMedicine and Pathology. was jointly mentored with ,Consultant in Endocrinology and the Transplant Center on a project evaluating

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Outcomes:Unknown

07/2009 - 10/2009

(Consultant) Description:July – Oct 2009 (3 month elective as part of the Master’s degreein Clinical Research, CTSA, Mayo Clinic, Rochester, MN). spent 3months in my laboratory as part of the elective – Introduction to LaboratoryMethods – to learn flow cytometry and immunology. He gained both practicallaboratory experience along with 10 hours of didactic discussions and mentoringin these areas. Outcomes:Consultant - Division of Endocrinology, Department of Medicine,Mayo Clinic-Rochester.

07/2009 - 10/2009



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(Neurology Fellow) Description:April 2009 (1 week elective rotation in Clinical Cellular andMolecular Immunology), Neurology Fellow, Dept of Neurology and Dept of LabMedicine and Pathology. rotation in my laboratory (Cellular &Molecular Immunology) was primarily focused on learning clinical immunology,clinical and laboratory diagnosis, interpretation and pathology of primaryimmunodeficiencies and transplant immunology. 07/2013 - Present: Collaborationand mentoring for a project related to immunophenotpying of B cells in patientswith autoimmune neurological disorders. Outcomes:Consultant, Department of Neurology, Department of LaboratoryMedicine and Pathology, Mayo Clinic - Rochester.

04/2009

(MS Student) Description:Member, MS Thesis Review Committee, Mayo Graduate School(thesis topic: role of alpha-defensins and NOD2 in the pathogenesis of ilealCrohn’s disease). was awarded his MS degree in 2010 from theMayo Clinic Graduate School. I was member of his Master's thesis committee. Outcomes:Quality specialist, Cellular & Molecular Immmunology ClinicalLaboratory, Department of Laratory Medicine and Pathology, Mayo Clinic -Rochester.

01/2009 - 01/2010

(Allergy/Immunology Fellow) Description:Dec – Jan 2009 (supervision of abstract preparation on casepresentation for the Primary Immunodeficiency Diseases Consortium meeting,

), Allergy/ Immunology Fellow, Division of Allergic Diseases, Dept ofMedicine. , Abraham RS.

braham RS.

Outcomes:Consultant, Allergist/Immunologist - Mayo Health System-

12/2008 - 01/2009

(Allergy/Immunology Fellow) Description:October 2008 (1 week elective rotation in Molecular Immunology),Allergy/ Immunology Fellow, Division of Allergic Diseases, Dept of Medicine. Inthis rotation, she was focusing on developing skills in molecular immunologyfocusing on an assay related to evaluating thymic reconstitution post-transplant. Outcomes:Private practice as an Allergist/Immunologist

10/2008

(Pathology Resident) Description:July 2008 (1 week elective rotation in Clinical Chemistry/ ClinicalPathology), Pathology Resident, Dept of Lab Medicine and Pathology.

rotation in my laboratory (Cellular & Molecular Immunology) wasprimarily focused on learning clinical and laboratory diagnosis, interpretation andpathology of primary immunodeficiencies. He reviewed primary immunodeficiencycases and sign-outs of patient reports. Outcomes:Pathologist, Anatomic and Clinical Pathology at

07/2008

(Graduate Student) Description:July 2008 (1 week rotation in Clinical Laboratory Immunology),MD student at the PhD student in the Mayo Graduate School.

focus during her week rotation in my laboratory (Cellular &Molecular Immunology) was learning the technical aspects of flow cytometry, flowdata analysis, specifically in the context of clinical immunology. Outcomes:Resident in Pediatric Neurology,

07/2008



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(Consultant) Description:July 2008 –Present, Division of Pulmonary Diseases, Dept ofMedicine, Mayo Clinic, Rochester, MN. has been working with meto develop a research project to use a flow cytometric immunologic approach toidentify patients with latent tuberculosis. The mentoring relationship has focusedon experimental design, laboratory support for initial flow experiments, support forgrant applications. This is an ongoing interaction and research project (Member,KO-8, KL2 and other research funding Advisory Committee). Outcomes:Consultant, Division of Pulmonary Diseases, Department ofMedicine, Mayo Clinic-Rochester.

01/2008 - Present

(Pathology Resident) Description:June 2007 (1 week elective rotation in Clinical Chemistry/ ClinicalPathology), Pathology Resident, Dept of Lab Medicine and Pathology. worked on a research project on molecular data analysis related to identifyingTACI genetic mutations in patients with common variable immunodeficiency(CVID) in my laboratory (Cellular & Molecular Immunology). Outcomes:Consultant, Department of Laboratory Medicine and Pathology,Mayo Clinic-Rochester.

06/2007

(Allergy/Immunology Fellow) Description:Mentored over the course of 1 year for discussions on careerplanning and clinical/patient issues related to primary immunodeficiencies, clinicalimmunology and transplant immunology. presented 2 lectures at theDivision of Allergy Case Conference related to the clinical discussion on twopatient cases:-

Outcomes:Consultant, Allergist/Immunologist -

01/2007 - 01/2008

(Senior Associate Consultant) Description:Clinical laboratory and research mentor for Department ofMedicine Faculty Development Award in my laboratory (Cellular & MolecularImmunology Laboratory), Department of Laboratory Medicine and Pathology.Research Project: Assessment of humoral immune responses and alterations inmemory B-cell populations in adult healthy individuals in response to thepneumococcal polysaccharide vaccine. Clinical laboratory mentor for PrimaryImmunodeficiencies. Laboratory), Dept of Lab Medicine and Pathology Dong XD,

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Outcomes:Consultant, Division of Allergic Diseases, Department of Medicine,

01/2007 - 01/2008



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Mayo Clinic - Rochester

(Pediatric Resident) Description:Pediatric Infectious Diseases/Pedeiatric Allergy & ImmunologyFellow, Division of Pediatric Infectious Diseases and Division of AllergicDiseases, Mayo Graduate School of Medicine. Member of thesisresearch committee for her Master's thesis in the Clinical and Research TrainingProgram (CRTP). Master’s Research Project:

Abraham RS,

Abraham RS.

,Abraham RS. .Manuscript in preparation. Abraham RS. Mayo Medical LaboratoriesWeb-based educational seminar on

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Outcomes:Consultant, Department of Pediatrics, Medicine and Lab Medicineand Pathology, Mayo Clinic-Rochester.

01/2007 - Present

(Ph.D. Student) Description:Ph.D. student laboratory rotation in my laboratory (Cellular &Molecular Immunology), Department of Laboratory Medicine and Pathology.Ongoing mentoring (since June 2007) as collaborator in research project onsepsis/SIRS in the neonatal and pediatric ICU. Research porject in lab:Development and clinical validation of multiparametric flow cytometry assay usingCD64, CD 163 and HLA DR along with cytokines for evaluating/differentiatingsepsis and non-infectioussystemic inflammation in the pediatric and neonatalintensive care unit (ICU). No presentations or publications hae come from thiswork as yet since this is an ongoing collaboration. The development of the flowcytometry assay will result in its availability as a clinical test through the Cellularand Molecular Immunology Lboratory for evaluating patients with SIRS and spesisupon completion of the clinical validation. AbrahamRS,

Outcomes:Completing M.D.-Ph.D. program, Mayo Medical School in .

01/2007 - Present

(Clinical Chemistry Fellow) Description:Sept –Dec 2006 (3 month, part-time research project in ClinicalImmunology), Clinical Chemistry Fellow, Dept of Lab Medicine and Pathology. .

worked on two clinical laboratory-related research projectsfocused on (1) developing and validating a flow cytometry assay for evaluatingCD40 expression on B-cells as a clinical diagnostic test, (2) developing andvalidating a functional component (flow cytometry) using a soluble chimericreceptor for a clinical diagnostic assay measuring expression of CD40L onactivated T-cells. These 2 assays are now part of the diagnostic flow cytometrypanel for Hyper IgM syndromes. Outcomes:Consultant - Division of Clinical Biochemistry and Immunology,Department of Laboratory Medicine and Pathology, Mayo Clinic-Rochester.

09/2006 - 12/2006



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Bellavia, Diego (Research Fellow) Description:Research co-mentor on the immunology/hematology portion on aproject related to a NIH R21 grant started in my laboratory in the Division ofHematology. Research project in lab: Myocardial Doppler imaging, strain rateanalysis and immunoglobulin variable gene usage in determining prognosis andoutcomes in patients with light chain amyloidosis (AL) following hematopoieticstem cell transplantation. Bellavia D, Abraham RS,

.

Bellavia D, Abraham RS,

Bellavia D, Abraham RS,

Outcomes:Last known position, Resident in Internal Medicine residencyprogram, .

08/2006 - 06/2010

(Pathology Resident) Description:June 2006 (3 week elective rotation in Clinical Chemistry/ ClinicalPathology), Pathology Resident, Dept of Lab Medicine and Pathology

worked on 2 projects in my laboratory (Cellular & MolecularImmunology): (1) he initiated clinical data retrieval in a database created forpatients seen in the Adult Immunodeficiency Clinic, (2) preliminary developmenton a flow cytometric assay to measure ICOS expression on activated T-cells. Outcomes:Consultant, Division of Hematopathology, Department ofLaboratory Medicine and Pathology, Mayo Clinic-Rochester.

06/2006

(Clinical Chemistry Fellow) Description:Co-supervised one of her research projects during fellowship. Outcomes:Consultant - Department of Laboratory Medicine and Pathology,Mayo Clinic

05/2006 - 06/2007

(Allergy/Immunology Fellow) Description:Allergy/Immunology fellow, Division of Allergic Diseases, MayoGraduate School of Medicine. Research mentor for work done in my laboratory(Cellular & Molecular Immunoology), Department of Laboratory Medicine andPathology. Abraham RS.

Outcomes:Last known position, Private Practice

04/2006 - 12/2006

(Graduate Student) Description:Graduate student enrolled in the Mayo Graduate School. Rotationthrough my laboratory in the Division of Hematology. No presentation sinceobjective of the rotation was to learn specific immunological techniques for use inher selected graduate laboratory. Outcomes:Present position unknown. Previously completed Ph.D. in theDepartment of Biochemistry and Molcular Biology, Mayo Graduate School.

01/2005



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(Summer Undergraduate Student) Description:Selected hrough the SURF (Summer Undergraduate ReserachFellowship) Program at Mayo Clinic for summer position in my laboratory in theDivision of Hematology. Poster presentation at the SURF Reserach and PosterPresentation Forum on: Development of a transgenic mouse expressingt anamyloidogenic human immunoglobulin light chain as a potential model for lightchain amyloidosis. Outcomes:Unknown

06/2004 - 08/2004

(Summer Undergraduate Student) Description:Selected through the SURF (Summer Undergraduate ResearchFellowship) Program at the Mayo Clinic for summer research position in mylaboratory in the Division of Hematology.

Abraham RS.

Abraham RS.

Outcomes:Post-doctoral fellow, Dept. of Cell Biology, .

06/2004 - 08/2004

(Postdoctoral Fellow) Description:Presentation of work done under my supervision in the Division ofHematology was given as a talk in partial fulfillment of the Master's thesisrequrements in Cellular and Molecular Biology form the

Abraham RS,

Abraham R,

Outcomes:Last known position - Completed Ph.D. in Department of Cell andMolecular Biology,

.

05/2003 - 10/2003

Institutional/Departmental Administrative Responsibilities, CommitteeMemberships and Other Activities

Mayo ClinicMayo Clinic Cancer Center

Associate Member

2002 - present

Mayo Clinic Center for Translational Science Activities

Associate Member, Rochester, Minnesota 2002 - Present

Mayo Clinic Clinical Research

Grant Reviewer 2005

Mayo Clinic in RochesterMayo Clinic Rochester Programs

Clinical Immunology and Immunotherapeutics Program

Core Faculty Member 2005 - 2011

This program has been discontinued at Mayo.



(b) (6)

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Department of Laboratory Medicine and Pathology

Research Committee

Division Representative 2007 - Present

Clinical Biochemical and Immunology Division

Flow Cytometry Validation Working Group

Chair 02/2010 -09/2010

Activities at Other InstitutionsRosalind Franklin Univeristy of Health Sciences and Northwestern University

Clinical Laboratory Immunology Fellowship Program

Reviewer, Chicago, Illinois 2011

Invited as external inspector by Committee for Postgraduate EducationPrograms, American College of Microbiology

National Institutes of Health

NIH Clinical Center

Department of Laboratory Medicine and Pathology

Reviewer, Bethesda, Maryland 2012

Invited as member of external review panel to inspect Department ofLaboratory Medicine at NIH Clinical Center

Presentations ExtramuralNational/International

Invited

Cell-based TherapiesCo-chairAssociation of Medical Laboratory Immunologists (AMLI)Annual MeetingSeattle, Washington

08/2014

Adult Primary Immunodeficiencies: expanding the spectrum and advances indiagnostic testing for PIDs.Immunodeficiency Think Tank Program at the Massachusetts General HospitalBoston, Massachusetts

06/2014

Update on newborn screening for SCID in the US and advanced diagnosticimmunology testing: breaking the frontiers.Immunodeficiency Think Tank Program at the Massachusetts General HospitalBoston, Massachusetts

06/2014

The national experience of newborn screening for Severe CombinedImmunodeficiencies (SCID) and T cell lymphopeniaPrimary Immunodeficiency Treatment Consortium MeetingSeattle, Washington

05/2014

New BiologicsCo-chairAssociation of Medical Laboratory Immunologists (AMLI)Annual MeetingSavannah, Georgia

08/2013



Translational research in newborn screening: Development and implementationof a tool for the clinical validation of Severe Combined Immunodeficiency (SCID)newborn screening.Lorey F, Abraham RS, Puck J, Caggana M, Rinaldo P, Brower A.Newborn Screening Translational Research Network (NBSTRN) MeetingBethesda, Maryland

04/2013

What's New in Testing for Primary ImmunodeficienciesSeminar series; Co-chair/presenterAAAAI annual meetingSan Antonio, Texas

02/2013

Advanced Laboratory Studies for Primary Immunodeficiency disordersProblem-based Learning workshop; Co-chair/presenterAAAAI annual meetingSan Antonio, Texas

02/2013

Immunodefiency Treatment and ManagementCo-ChairAssociation of Medical Laboratory Immuologists (AMLI) Annual MeetingSan Diego, California

08/2012

Diagnostic testing for Primary immunodeficienciesCo-chair/presenterAAAAI annual meetingOrlando, Florida

03/2012

Immune monitoring approaches for transplantationDept of Medicine Lecture, Christian Medical College, Punjab, IndiaLudhiana, Punjab, India

10/2011

Advances in diagnostic approaches for primary immunodeficienciesGrand Rounds, Department of Medicine, Christian Medical College, Punjab, IndiaLudhiana, Punjab, India

10/2011

Advances in Primary ImmunodeficienciesCo-chairAssociation of Medical Laboratory Immunologists (AMLI) Annual MeetngSan Diego, California

08/2011

Case Studies in Clinical ImmunologyCo-ChairAssociation of Medical Laboratory Immuologists (AMLI) Annual MeetingSan Diego, California

08/2011

Case Studies in Clinical ImmunologyCo-chairAssociation of Medical Laboratory Immunologists (AMLI)Orlando, Florida

08/2010

Clinical immunology and diagnostic testing for primary immunodeficienciesGrand Rounds, Department of Laboratory Medicine, Yale University School ofMedicineNew Haven, Connecticut

04/2010

Understanding Clinical Laboratory ImmunologyLecture 1 of 3Baptist Mission Hospital, Cote d'IvoireFerkessedougou, Cote D'Ivoire

09/2009



Laboratory Testing for Diabetes and Liver DiseasesLecture 2 of 3Baptist Mission Hospital, Cote d'IvoireFerkessedougou, Cote D'Ivoire

09/2009

Immunology Testing for Infectious DiseasesLecture 3 of 3Baptist Mission Hospital, Cote d'IvoireFerkessedougou, Cote D'Ivoire

09/2009

Primary Immunodeficiencies and Flow CytometryAssociation of Medical Laboratory Immunologists (AMLI) Annual MeetingBoston, Massachusetts

08/2009

Primary Immunodeficiencies: Models for Understanding Human ImmunobiologyJoint Symposium of the Association of Medical Laboratory Imunnologists(AMMLI)and the American Association of Immunologists (AAI)Seattle, Washington

05/2009

Well-defined Immunodeficiency diseasesCo-ChairPan-American Meeting on Primary Immunodeficiencies Meeting CincinnatiChildren's HospitalCincinnati, Ohio

11/2008

DiGeorge Syndrome: The T-cells have a story to tell!Association of Medical Laboratory Immunologists Annual MeetingSeattle, Washington

08/2008

Relevance and role of immunological monitoring and assessment of immunereconstitution in clinical trialsInternational Society of Cell Therapy Annual MeetingMiami, Florida

05/2008

Clinical Immunology in Underserved Areas: Relevance and Clinical UtilityKibogora Mission Hospital, RwandaKibogora, Rwanda

10/2007

Basic Laboratory Diagnotic Testing in a Community Hospital SettingKibogora Mission Hospital, RwandaKibogora, Rwanda

10/2007

New advances in understanding the pathogenesis of primary systemicamyloidosis (AL)Grand Rounds, Department of Medicine, Worcester Medical Center and St.Vincent's HospitalWorcester, Massachusetts

05/2005

Understanding the Immunobiology of Primary Light-chain Amyloidosis (AL)Grand Rounds, Department of Laboratory Medicine and Pathology GrandRounds, University of WashingtonSeattle, Washington

01/2005

The Clinical and Immunological Spectrum of Light-chain Amyloidosis (AL)Medical School Lecture, University of Washington Medical SchoolSeattle, Washington

01/2005

Serum free light chains for diagnosis and monitoring patients with light chainamyloidosisThe Binding Site Symposium on Free Light ChainsBirmingham, United Kingdom

03/2001

Oral



Rapid - onset fatal EBV-associated hemophagocytic lymphohistiocytosis2nd Annual Clinical Immunology Society MeetingChicago, Illinois

04/2012

An Edu®-based flow cytometry assay to nmeasure lymphocyte proliferation in theclinical laboratory: replacement of the "gold standard" 3H-thymidine method.Great Lakes International Imaging and Flow Cytometry Association (GLIIFCA)meetingMilwaukee, Wisconsin

10/2010

Anti-TNFa therapy results in marginal zone B cell upregulation in human Crohn'sdiseaseKhanna S, Abraham RS, Faubion W.Digestive Diseases WeekNew Orleans, Louisiana

05/2010

Decrease in class-switched memory B cells is associated with malignancy incommon variable immunodeficiency (DVID)FOCIS Annual meeting and PID Consortium meetingSan Francisco, California

06/2009

Accumulation of specific memory B-cell subsets in peripheral blood of healthyadults in response to pneumococcal vaccinationXIIIth meeting of the European Society for Immunodeficiencies's-Hertogenbosch, Netherlands

11/2008

Assessmemt of thymic reconstitution in the clinical laboratory using TRECs as amarkerKeystone Symposium on Immunological Intervention in Human DiseaseBig Sky, Montana

01/2007

Development and validation of a clinical assay to measure thymic reconstitutionand output using T cell receptor excision circles(TREC) by real-time PCR19th Annual Meeting of the Association of Medical Laboratory Immunologists(AMLI)Washington, District of Columbia

08/2006

Analysis of serum immunoglobulin free light chains in primary systemicamyloidosis and light chain deposition disease by nephelometryIXth Int Symp. on AmyloidosisBudapest, Hungary

07/2001

Utility of serum free light chain measurements for assessing response followingautologous stem cell transplantation for primary amyloidosisIXth International Symposium on AmyloidosisBudapest, Hungary

07/2001

T and B cell epitope mapping of human RO60 in HLA DR 2 and DR3 transgenicmiceLupus Foundation of America MeetingBethesda, Maryland

09/1997

Poster

Unusual presentation of possible NCF4-associated Chronic GranulomatousDisease (CGS) in two siblingsInternational Primary Immunodeficiency CongressLisbon, Portugal

11/2013



Familial, non-zinc finger region, GATA2-mutated myelodysplastic syndrome:associated with shortened telomere lengths, viral warts, profound monocyte, Band NK cell deficieincy.Patnaik M, Jackson A, Abrahan RS, Rodriguez V, Howard M, Hsu A, Holland S.European Society of Hematology MeetingStockholm, Sweden

06/2013

Clinical spectrum and outcomes of patients with inherited bone marrow failuresyndromes: a Mayo Clinic series of 55 patients.Kenderian SS, Litzow MR, Gastineau DA, Letendre L, Rodriguez V, Gourde J,Khan SP, Hogan WJ. Abraham RS, Patnaik MM.European Society of Mematology MeetingStockholm, Sweden

06/2013

Abnormal neutrophil oxidaztive burst (NOXB) in a 72y old male with secondaryhemophagocytic lymphohistiocytosis (HLH).Samant S, Joshi AY, Abraham RS.Clinical Immunology Society Annual MeetingMiami, Florida

04/2013

Whole-exome sequencing for identification of a potential novel defect in a patientwith rapid-onset fatal virus-associated hemophagocytic syndrome (VAHS).Abraham RS,Dong X, Eckloff B, Middha S, Bram R.European Society of Immunodeficiencies (ESID) MeetingFlorence, Italy

10/2012

Validation and generation of adult and pediatric reference values for a new 7-colorT, B and NK quantation assayAbraham RS, League S, Dong X, Jenkins S.International Society of Flow Cytometry MeetingLeipzig, Germany

06/2012

Evaluation of a novel flow cytometric method for the diagnosis of latenttuberculosis infection.Escalante P, Peikert T, Erskine C, Bornhorst C, McKey C, Andreist B, BinnickerM, Feske M, Graviss E, Abraham RS, Limper AH, Knutson KL.American Thoracic Society MeetingSan Francisco, California

05/2012

Translational research in newborn screening: Development of a tool for theclinical validation of Severe Combined Immunodeficiency (SCID) NewbornScreening.Lorey F, Abraham RS, Puck J, Caggana M,Rinaldo P, Brower A.American College of Medical Genetics Annual MeetingCharlotte, North Carolina

03/2012

An atypical and novel presentation of Omenn syndrome due to ADA deficiency ina Somali infantHam E, Abraham RS, Khan S, SHah NB, Joshi AJAmerican College of Allergy, Asthma and ImmunologyBoston, Massachusetts

11/2011

Pulmonary manifestations in patients with common variable immunodeficiencyNelson DR, Moua T, Midthun D, Abraham RS, Vassallo RAmerican College of Chest Physicians Annual MeetingHonolulu, Hawaii

10/2011

Clinical utility of a quantitative and functional flow cytometry assay forCMV-specific CD8 T cell immunity in transplant patientsDong XD, Abraham RS.European Society of Organ Transplantation (ESOT) MeetingGlasgow, Scotland

09/2011



Molecular characterization of a novel BTK mutation (C502Y) in a patient withX-linked agammaglobulinemia.Dong XD, Hagan JB, Abraham RSFederation of Clinical Immunology Societies (FOCIS) Annual MeetingWashington, District of Columbia

06/2011

Epidemiology of CMV (cytomegalovirus) infection after pancreas transplantationParsaik A, Kremers W, Abraham RS, Razonable RR, Kudva YS.International Pancrease and Islet Transplantation Association (IPITA) MeetingPrague, Czech Republic

06/2011

A clinically validated flow cytometric method using Edu to measure lymphocyteproliferative responses: alternatives for the clinical immunology laboratoryErickson JR, Abraham RS2nd Annual Clinical Immunology Society MeetingChicago, Illinois

05/2011

Assessment of the association of asthma status with selective IgA deficiency andcommon variable immunodeficiency: a population-based control studyUrm S-H, Yun HD, Fenta Ya, Yoo KH, Abraham RS, Hagan JB, Juhn YjAmerican Academy of Allergy, Asthma and Immunology Annual MeetingSan Francisco, California

03/2011

Analytical and clinical validation of a diagnostic test for evaluation of NK and NKTcell subsetsAbraham RS, Dong X.Annual Meeting of the American Academy of Allergy, Asthma, and ImmunologySan Francisco, California

03/2011

Efficacy, durability and immunological analyses following Rituximab therapy inidiopathic membranous nephropathy: a 2 year study.Fervenza F, Abraham R, Erickson SB, Irazabal-MIra MV, Eirin A, Specks U,Nachman PH, Bergstralh EJ, Leung N, Cosio FG, Hogan M, Dillon JJ, Hickson LJ,Cattran DC.American Society of Nephorologu - Renal Week meetingDenver, Colorado

11/2010

Molecular characterization of a novel BTK mutation (C502Y) and identification ofadditional unreported BTK mutations in X-linked agammaglobulinemia (XLA)Dong X, Hagan JB, Yilmaz-Demirdag Y, Pozos T, Bahna S, Kobrynski L,Abraham RS.XIVth Meeting of the European Society for Primary Immunodeficiencies (ESID)Istanbul, Turkey

10/2010

Development and validation of a multicolor flow cytometry assay for clinicallaboratory assesment of CD4 recent thymic emigrants (RTEs).Dong X, Hoeltzle MV, Abraham RS.American Association for Clinical Chemistry (AACC) Annual MeetingAnaheim, California

07/2010

Assessment of the association of asthma status with IgA deficiency and commonvariable dificiency disorders: a population-based case control studyUrm S-H, Yun HD, Fenta YA, Abraham RS, Hagan J, Juhn YJ.European Academy of Allergy and Clinical Immunology (EAACI)London, England

06/2010

Use of the Electronic Medical Record (EMR) and clinical and translational science(CTSA) resources for research involving biomarkers of sepsis in critically illneonates and childrenJuskewitch JE, Prasad S, Grande JP, Abraham RS, Huskins WC.Pediatric Academic Societies (PAS)Vancouver, British Columbia, Canada

06/2010



Serological CMV mismatch in pancrease transplantation recipients posesseve-fold increased risk of CMV infectionBhalla T, ROstambeigi N, Dean P, Prieto M, Cosio F, Abraham R, Kudva Y.American Transplant CongressSan Diego, California

05/2010

Immune monitoring demonstrates profound immunological anomalies in pancreasafter kidney (PAK) transplant recipientsKudva YC, Rostamebeigi N, Erickson JR, Abraham RS.American Transplant CongressSan Diego, California

05/2010

Two brothers with hypogammaglobulinemia and normal Btk protein expressionYilmaz-Demirdag Y, Dong X, Hackett M, Abraham RS, Ochs HD, Bahna S.First North American Conference on Primary ImmunodefienciesPhiladelphia, Pennsylvania

05/2010

Assessment of CMV CD8 T cell immunity in renal transplant patientsDong XD, Kudva Y, Abraham RS.IXth International Conference on New Trends in Immunosuppression andImmunotherapyGeneva, Switzerland

02/2010

Quantitation of T and B cell subsets do not predict response to Rituximab therapyin patients with idiopathic membranous nephropathy: a prospective studyEirin A, Irazabal MV, Abraham RS, Erickson SB, Dillon JJ, Nachman P, CattranDC, Fervenza FC.American Society of Nephrology meetingSan Diego, California

10/2009

A compound heterozygote mutation in the ITGB2 gene resulting in leukocyteadhesion deficiency type 1(LAD-1)Shah KM, Pratt EL, Abraham RS.PID Consortium meetingSan Francisco, California

06/2009

Pulmonary Alveolar Proteinosis (PAP) with recurrent infections: a case if primaryimmunodeficiency?Iyer VN, Joshi AY, Lewis JC, Abraham RS.PID Consortium meetingSan Francisco, California

06/2009

CD4 and CD8 recent thymic emigrants (RTEs) analysis: validation of a clinicaldiagnostic multi-color flow cytometric assay for assessment of thymic outputDong X, Hoeltzle MV, Abraham RS.Keystone Symposium on Primary ImmunodeficienciesBeijing, China

05/2009

Evaluation of the frequency and phenotypic heterogeneity of the TNFRSF13B(TACI) A181E mutation in a cohortof CVID and lymphoma patientsDong X, Hoeltzle M, Nandakumar V, Dunker J, Ansell S, Bram R, Hagan J, ParkM, Abraham RS.FOCIS annual Meeting and PID Consortium MeetingBoston, Massachusetts

06/2008

Elevated serum immunoglobulin E: when to suspect Hyper IgE syndromeJoshi AY, Boyce TG, Abraham RS, Hagan JB, Park MA.Pediatric Academic Societies' & Asian Society for Pediatric Research JointMeetingHonolulu, Hawaii

05/2008



Identification of putative CTL epitopes on BK virus VP1 protein for assessment ofimmune competence in transplant-associated BK nephropathyAbraham RS.American Academy of Asthma, Allergy and ImmunologyPhiladelphia, Pennsylvania

03/2008

Resolution of hypogammaglobulinemia after resection of renal cell carcinomaMcClimon BJ, Abraham RS, Park MA, Hagan JB.American College of Allergy, Asthma and Immunology Annual MeetingDallas, Texas

11/2007

Development of a quantitative flow cytometric assay for assessment of functionalCMV-specific CD8 T-cell immune competence in the clinical laboratoryNandakumar, V, Abraham, RS.13th International Congress of ImmunologyRio de Janeiro, Brazil

08/2007

Clonal B cells in blood and bone marrow of patients with a plasma cell dyscrasia-light chain amyloidosisManske MK, Maiden S, Abraham RS.FOCIS Annual MeetingBoston, Massachusetts

05/2005

Circulating soluble light chain oligomers in sera of patients with light chainamyloidosisHenon KT, Dispenzieri A, Katzmann JA, Lacy MQ, Ramirez M, Gertz MA, KyleRA, Abraham RS.Xth International Myeloma MeetingSydney, Australia

04/2005

Clonal B cells in blood and bone marrow of patients with a plasma cell dyscrasia-light chain amyloidosisManske MK, Maiden S, Abraham RS.Keystone Symposia on B Cell Immunology

03/2005

Molecular classification of light chain (AL) amyloidosis by gene expressionprofilingAbraham RS, Manske M, Ballman K, DIspenzieri A, Price-Troska T, Gertz MA,Fonseca R.Proceedings of the Xth International Symposium on Amyloid and AmyloidosisTours, France

04/2004

Analysis of B cell clonal evolution in BM and PBL sequences of AL and MMpatients using graphical quantification of linage treesAbraham RS, Manske MK, Sohni A, Edelman H, Zuckerman N, Shahaf G,Dispenzieri A, Lacy MQ, Gertz MA, Mehr R.Xth International Symposium on Amyloid and AmyloidosisTours, France

04/2004

Regulation of SDF-1-CXCR4 interactions by SOCS3 in myeloma and MGUSbone marrowGonzalez-Paz N, Manske M, Price-Troska TL, TImm M, Ahmann G, Fonseca R,Abraham RS.IXth International Workshop on Multiple MyelomaSalamanca, Spain

05/2003

Immunoglobulin variable (V) region germline genes and mutational hotspotspotentially influence protein misfolding in light chain amyloidosis (AL)Abraham RS, Geyer SM, Ramirez-Alvarado M, Price-Troska TL, Allmer C, KyleRA, Gertz MA, Fonseca R.American Society for Hematology Meeting

12/2002



Gene expression profiling of clonal plasma cells in light chain (AL) amyloidosis bymicroarray analysisAmerican Society for Hematology Meeting

12/2002

Correlation of serum immunoglobulin free light chain quantification with urinaryBence Jones protein in light chain myelomaAbraham RS, Clark RJ, Bryant SC, Lump JF, Larson T, Kyle Ra, Katzmann JA.American Association of Clinical Chemistry Meeting

07/2002

Immunoglobulin light chain variable (V) region gene usage determines organtropism and overall survival in light chain-associated (AL) amyloidosisAbraham RS, Price-Troska TL, Allmer C, Geyer SM, Kyle RA, Gertz MA, FonsecaR.Summer FASEB MeetingSnowmass, Colorado

06/2002

Characterization of free immunoglobulin light chains (LC) by mass spectrometryin light chain-associated (AL) amyloidosisAbraham RS, Bergen HR, Naylor S, Katzmann JA, Bradwell AR, Kyle RA,Fonseca R.American Society for Hematology Annual Meeting

12/2001

Association of rearranged light chain variable (V) region genes with organ tropismin light chain-associated (AL) amyloidosisAbraham RS, Price-Troska TL, Gertz MA, Kyle RA, Fonseca R.American Society for Hematology Annual Meeting

12/2001

Detection of serum immunoglobulin free light chains in primary amyloidosis andlight chain deposition disease by nephelometryAbraham RS, Katzmann JA, Clark RJ, Dispenzieri A, Lust JA, Bradwell AR, KyleRA.American Association of Clinical Chemistry Meetng

07/2001

Light chain myeloma: Correlation of serum nephelometic analysis for thequantitation of immunoglobulin free light chain with urine Bence Jones proteinAbraham RS, Katzmann JA, Clark RJ, Lymp JF, Dispenzieri A, Lust JA, BradwellAR, Kyle RA.American Association of Clinical Chemistry Meetng

07/2001

Analysis of immunoglobulin light chains in primary amyloidosis --direct on-lineassay for amyloidogenic Kappa and Lambda light chains directly from serumNaylor S, Abraham RS, Bradwell AR, Fonseca R, Katzman J, Kyle RA, BergenHR III.IXth Intl Symp on AmyloidsisBudapest, Hungary

07/2001

Spontaneous T-cell autoreactivity to rat GAD65 in HLA transgenic mice with Type1 diabetes-susceptible MHC haplotypes but not with diabetes-resistant haplotypesAbraham RS, Wen L, David CS.American Diabetes Association Annual MeetingSan Antonio, Texas

05/2000

Modulation of insulitis and type 1 diabetes in NOD mice lacking MHC class IIgenes in the presence or absence of HLA DR3Kudva YC, Abraham RS, David CS.American Diabetes Association MeetingSan Antonio, Texas

05/2000

HLA transgenic mice as models for Type 1 diabetes mellitusJuvenille Diabetes Association Annual MeetingLansdowne, Virginia

03/2000



Type 1 diabetes-susceptible and resistant HLA Class II molecules modulateinsulitis in transgenic miceAbraham RS, Kudva YC, David CS.American Diabetes Association Annual MeetingSan Diego, California

05/1999

T cell responses to GAD65 in NOD/DQ8/A beta knock-out transgenic mice: roleof background genesAbraham RS, Wilson SB, Strominger JL, Munn SR, David CS.American Diabetes Association Annual MeetingChicago, Illinois

05/1998

Regional

Invited

Update newborn screening for SCID and T cell lymphopenia in MN and nationally.Immune Deficiency Foundation Minnesota Chapter MeetingSt. Paul, Minnesota

10/2013

Genetic Testing for Primary ImmunodeficienciesImmune Deficiency Foundation Minnesota Chapter MeetingSt. Paul, Minnesota

08/2012

Immune Monitoring for Organ and Bone Marrow Transplantation: Role of theClinical LaboratoryClinical Laboratory Collaborative for Minnesota Annual Meeting talkRochester, Minnesota

05/2009

Laboratory testing for CVID and other humoral immunodeficienciesImmune Deficiency Foundation Minnesota Chapter meeting

07/2008

Clinical Practice, Interests, and AccomplishmentsINTERESTS Primary immunodeficiencies - diagnosis, managment and understanding the pathophysiology ofprimary immunodeficiencies, study of the underlying genetic defects, effect of particular immunologicaldefects on components and function of the immune system, laboratory and clnical correlation betweenimmunological tests and specific primary immunodeficiency phenotypes

Transplant immunology - understanding the effects of immunosupresion on immune competence andimmunological reconstitution, using laboratory -based immunological testingt to develop personalizedimmunosupressive regimens, reducing infectious co-morbidity due to immunosuprresion

Immune monitoring - serial and systematic evaluation of immune reconstitution and functional recoveryin relevant clinical contexts, e.g. use of biological immuno-modulatory drugs, immunotherapy orchemotherapy for the treatment of autoimmunity, cancer, transplatation

ACCOMPLISHMENTS

Primary immunodeficiency clinical laboratory (Cellular and Molecular Immunology) - The primaryimmunodeficiencyu clinical diagnostic laboratory was started in July 2005 and has since thensupported the practice of the Adult Immunodeficiency Cllinic and Pedicatric Immunodeficiency Clinicwithin the Division of Allergic Diseases and Dept of Pediatrics respectively. In the past, certainimmunodeficiency-related tests were done within the Hematopathology clinical laboratory; however,the setting of a clinical laboratory whose emphasis is on primary immunodeficiencies has allowedexpansion of our diagnostic capabilities in this area both for our internal and extramural practice. Since2005, approximately 30 new or modified clinical flow cytometry, cellular and molecular tests related toprimary immunodeficiencies or the assesment of immune competence have been developed andimplemented or will be implemented. The laboratory is constantly expanding its test "menu" forprimary immunodeficiencies and evaluation of immune competence.

The clinical laboratory, besides supporting the immunodeficiency practices, also provides a keyresource for the clinical and research assessmen tof immune reconstitution and immune competence



in several other clinical contexts, including autoimmune diseases, hematological Malignancies,solid-organ transplantation and bone marrow (stem cell) transplantation.

Mulidisciplinary clinical collaborations - Collaboration with clinicians in Pediatrics, Hematology,Gastroenterology, Rheumatology, Allergic Diseases, Transplantation, Cardiology, Nephrology,Infectious Diseases to support clinical use and interpretatin of immunological tests, implementation ofclinical research protocols to test and improve clinical practice and patient care related to reducinginfectious co-morbidities and evidence-based use of immunological intervention or therapy

Research InterestsPrimary immunodeficiencies - Identifying and developing state-of-the-art techniques to daignoseprimary immunodeficiencies, study of the pathophysiology of common variable immunodeficiency(CVID), identification of genotype-phenotype corerlations with TACI gene mutatins in CVID, laboratorycorrelation of specific immunological parameters with clinical presentation in CVID and other humoralimmunodeficiencies, identifying new methods to improve diagnostic criteria for impaired functionalantibody responses in humoral immunodeficiencies. Transplant immunology - Evaluation of immune reconstitution and assessmen tof immune competiencein hematopoietic stem cell transplantation, the effect of various conditioning regiments on immunereconstitution, the use of immune reconstitution and immune competence as the criteria for directingvaccination strategies post-transplant, developing evidence-based strategies based on immunologicalfunction to determine immunosuppression in solid-organ transplant recepients.

Immunobiology of primary systemic (light chain) amyloidosis - studing immunoglobulin light chainvariable gene usage in light chain amyloidosis, the effect of light chain gene usage on survivalpost-hematopoietic stem cell transplant, light chain gene usage and prediction of cardiac dysfunction inprimary amyloidosis.

Identification of biomarkers for sepsis and systemic inflammatory response syndrome (SIRS) - use ofquantitative CD64 expression on neutrophils and HLA DR and CD163 on monocytes to differentiatiebetween sepsis and SIRS in the intensifve care unit setting, evaluation of immunosuppressivemonocytes in neoplastic diseases.

Research Grants AwardedActive Grants

OtherCo-ProgramDirector /PrincipalInvestigator

Development and Implementation of Newborn Screening forSevere Combined Immune Deficiency Screening in Minnesota.Funded by Centers for Disease Control and Prevention. (U01EH000930-01)

10/01/2011 -10/01/2013

Completed GrantsFederalPrincipalInvestigator

Modulation of Cardiac Mechanics in AL Amyloidosis. Funded byNational Heart, Lung, and Blood Institute. (R21 HL 76513)

09/2004 - 08/2007

FoundationPrincipalInvestigator

The Contribution of HLA Class II Genes in the Analysis of theGenetic Component of Systemic Lupus Erythematosus. Fundedby Lupus Foundation of America, Inc., Minnesota Chapter.(Lupus #11)

01/1998 - 12/1999

PrincipalInvestigator

Immune Responses of NOD/DQB Transgenic Mice to GlutamicAcid Decarboxylase (GAD) and Its Relevance to HumanInsulin-Dependent Diabetes Mellitus. Funded by JuvenileDiabetes Foundation International. (JDF #12)

06/1998 - 06/2000


The Contribution of HLA Class II Genes in the Analysis of theGenetic Component of Systemic Lupus Erythematosus. Fundedby Arthritis Foundation North Central Chapter.

07/1998 - 06/1999

Co-Investigator Research grant. Funded by American Diabetes Association. 01/2000 - 01/2003



Mayo ClinicProgramDirector /PrincipalInvestigator

Study of the Mechanisms of AMyloid Formation and Depositionin AL AMyloidosis Study of the Mechanisms of AMyloidFormation and Deposition in Light chain Amyloidosis. Funded byDepartment Discretionary Funds and the HematologicMalignancies Fund.

07/2002 - 07/2005


Study of the Mechanisms of Amyloid Formation and Depositionin AL Amyloidosis. Funded by Department Discretionary Funds.

01/2003 - 12/2004

ProgramDirector /PrincipalInvestigator

Functional and biochemical characterization of the novel pointmutation - C502Y in the Btk gene in a patient with X-linkedagammaglobulinemia. Funded by Small Grants Award, Divisionof Allergic Diseases, Department of Internal Medicine.

04/2008 - 04/2010

Co-ProgramDirector /PrincipalInvestigator

Towards personalized immunosuprression for pancreasetransplantation: development of an evidence-based strategy.Funded by Department of Laboratory Medicine and Pathology.

08/2008 - 08/2009

Co-ProgramDirector /PrincipalInvestigator

Evaluating immunosuppressive strategies in pancreastransplantation and development of immune monitoringmodalities. Funded by Division of Endocrinology.

01/2009 - 12/2009

Co-Investigator Identification and Characterization of ImmunosuppressiveMonocytes. Funded by AS-Administrative Services.

02/2009 - 01/2010

PatentsTitle Patent Number Date filed Date issued

Methods to increase and modify immuneresponsesInventors: Rath, S., Abraham, R. S., Singh, N.,Basu, S. K., Mukhopadhyay, A., and Bal, V.

EP9630846371270

11/1997

Method to increase and modify immuneresponsesInventors: Rath, S., Abraham, R. S., Singh, N.,Basu, S. K., Mukhopadhyay, A., and Bal, V.

AU 698380 03/1996 04/1997



BibliographyPeer-reviewed Articles

Tebo AE, Detrick B, Hamilton RG, Khanolkar A, O'Gorman MR, Schmitz JL, AbrahamRS . Clinical Laboratory Immunology: An Indispensable Player in Laboratory Medicine.Am J Clin Pathol. 2014 Oct; 142(4):437-44. PMID:25239409. DOI:10.1309/AJCPX25MFWNEYRIG.

1.

Kwan A, Abraham RS , Currier R, Brower A, Andruszewski K, Abbott JK, Baker M,Ballow M, Bartoshesky LE, Bonilla FA, Brokopp C, Brooks E, Caggana M, Celestin J,Church JA, Comeau AM, Connelly JA, Cowan MJ, Cunningham-Rundles C, Dasu T,Dave N, De La Morena MT, Duffner U, Fong CT, Forbes L, Freedenberg D, Gelfand EW,Hale JE, Hanson IC, Hay BN, Hu D, Infante A, Johnson D, Kapoor N, Kay DM, Kohn DB,Lee R, Lehman H, Lin Z, Lorey F, Abdel-Mageed A, Manning A, McGhee S, Moore TB,Naides SJ, Notarangelo LD, Orange JS, Pai SY, Porteus M, Rodriguez R, Romberg N,Routes J, Ruehle M, Rubenstein A, Saavedra-Matiz CA, Scott G, Scott PM, Secord E,Seroogy C, Shearer WT, Siegel S, Silvers SK, Stiehm ER, Sugerman RW, Sullivan JL,Tanksley S, Tierce ML 4th, Verbsky J, Vogel B, Walker R, Walkovich K, Walter JE,Wasserman RL, Watson MS, Weinberg GA, Weiner LB, Wood H, Yates AB, Puck JM.Newborn screening for severe combined immunodeficiency in 11 screening programs inthe United States. JAMA. 2014 Aug 20; 312(7):729-38. PMID:25138334.DOI:10.1001/jama.2014.9132.

2.

Urm SH, Yun HD, Fenta YA, Yoo KH, Abraham RS , Hagan J, Juhn YJ. Asthma and riskof selective IgA deficiency or common variable immunodeficiency: a population-basedcase-control study. Mayo Clin Proc. 2013 Aug; 88(8):813-21. PMID:23910409. PMCID:3753684.DOI:10.1016/j.mayocp.2013.05.021.

3.

Gustafson MP, Lin Y, LaPlant B, Liwski CJ, Maas ML, League SC, Bauer PR, AbrahamRS , Tollefson MK, Kwon ED, Gastineau DA, Dietz AB. Immune monitoring using thepredictive power of immune profiles. Journal for ImmunoTherapy of Cancer. 2013 Jun27; 1(7). DOI:10.1186/2051-1426-1-7.

4.

Kudva YC, Erickson JR, Parsaik A, Rostambeigi N, Thapa P, Abraham RS .Comprehensive immune monitoring reveals profound immunological changes inpancreas after kidney (PAK) transplant recipients. Hum Immunol. 2013 Jun;74(6):738-45. Epub 2013 Mar 07. PMID:23474226. DOI:10.1016/j.humimm.2013.02.004.

5.

* Juskewitch JE, Enders FT, Abraham RS , Huskins WC. Novel infrastructure for sepsisbiomarker research in critically ill neonates and children. Clin Transl Sci. 2013 Feb;6(1):21-5. Epub 2013 Jan 14. PMID:23399085. PMCID:3578308. DOI:10.1111/cts.12025.

6.

Tschumper RC, Dispenzieri A, Abraham RS , Henderson KJ, Jelinek DF. Molecularanalysis of immunoglobulin genes reveals frequent clonal relatedness in doublemonoclonal gammopathies. Blood Cancer J. 2013; 3:e112. Epub 2013 Apr 19.PMID:23599024. PMCID:3641320. DOI:10.1038/bcj.2013.12.

7.

Trushin SA, Carena AA, Bren GD, Rizza SA, Dong X, Abraham RS , Badley AD.SDF-1alpha degrades whereas glycoprotein 120 upregulates Bcl-2 interacting mediatorof death extralong isoform: implications for the development of T cell memory. JImmunol. 2012 Aug 15; 189(4):1835-42. Epub 2012 Jul 16. PMID:22802411. PMCID:3530261.DOI:10.4049/jimmunol.1100275.

8.

Abraham RS . Relevance of antibody testing in patients with recurrent infections. JAllergy Clin Immunol. 2012 Aug; 130(2):558-9.e6. PMID:22846755. DOI:10.1016/j.jaci.2012.06.013.

9.

* Podjasek JC, Abraham RS . Autoimmune cytopenias in common variableimmunodeficiency. Front Immunol. 2012; 3:189. Epub 2012 Jul 24. PMID:22837758.PMCID:3402902. DOI:10.3389/fimmu.2012.00189.

10.

Katoh N, Poshusta TL, Manske MK, Dispenzieri A, Gertz MA, Abraham RS ,Ramirez-Alvarado M. A reappraisal of immunoglobulin variable gene primers and itsimpact on assessing clonal relationships between PB B cells and BM plasma cells in ALamyloidosis. J Clin Immunol. 2011 Dec; 31(6):1029-37. Epub 2011 Sep 10. PMID:21909811.

11.



PMCID:3224190. DOI:10.1007/s10875-011-9582-y.

Parsaik AK, Bhalla T, Dong M, Rostambeigi N, Dierkhising RA, Dean P, Abraham R ,Prieto M, Kremers WK, Razonable RR, Kudva YC. Epidemiology of cytomegalovirusinfection after pancreas transplantation. Transplantation. 2011 Nov 15; 92(9):1044-50. PMID:21904269. DOI:10.1097/TP.0b013e31823015c1.

12.

* Joshi AY, Abraham RS , Snyder MR, Boyce TG. Immune evaluation and vaccineresponses in Down syndrome: evidence of immunodeficiency? Vaccine. 2011 Jul 12;29(31):5040-6. Epub 2011 May 17. PMID:21596078. PMCID:3909669.DOI:10.1016/j.vaccine.2011.04.060.

13.

* Bellavia D, Abraham RS , Pellikka PA, Dispenzieri A, Burnett JC Jr, Al-Zahrani GB,Green TD, Manske MK, Gertz MA, Miller FA Jr, Abraham TP. Utility of Dopplermyocardial imaging, cardiac biomarkers, and clonal immunoglobulin genes to assess leftventricular performance and stratify risk following peripheral blood stem celltransplantation in patients with systemic light chain amyloidosis (Al). J Am SocEchocardiogr. 2011 Apr; 24(4):444-54. Epub 2011 Feb 18. PMID:21315556. PMCID:3065954.DOI:10.1016/j.echo.2011.01.003.

14.

Abraham RS . Relevance of laboratory testing for the diagnosis of primaryimmunodeficiencies: a review of case-based examples of selected immunodeficiencies.Clin Mol Allergy. 2011; 9:6. Epub 2011 Apr 09. PMID:21477322. PMCID:3080807.DOI:10.1186/1476-7961-9-6.

15.

* Shah KM, Pratt EL, Al-Rahawan MM, Abraham RS . Novel combination of ITGB2mutations causing leukocyte adhesion deficiency type 1 (LAD-1). Journal of PediatricInfectious Diseases. 2011; 6(2):141-8.

16.

Fervenza FC, Abraham RS , Erickson SB, Irazabal MV, Eirin A, Specks U, NachmanPH, Bergstralh EJ, Leung N, Cosio FG, Hogan MC, Dillon JJ, Hickson LJ, Li X, CattranDC, Mayo Nephrology Collaborative Group. Rituximab therapy in idiopathic membranousnephropathy: a 2-year study. Clin J Am Soc Nephrol. 2010 Dec; 5(12):2188-98. Epub2010 Aug 12. PMID:20705965. PMCID:2994079. DOI:10.2215/CJN.05080610.

17.

Rostambeigi N, Kudva YC, John S, Mailankody S, Pedersen RA, Dean PG, Prieto M,Cosio FG, Kremers WK, Walker RC, Abraham RS , Stegall MD. Epidemiology ofinfections requiring hospitalization during long-term follow-up of pancreas transplantation.Transplantation. 2010 May 15; 89(9):1126-33. PMID:20164817.DOI:10.1097/TP.0b013e3181d54bb9.

18.

* Dong X, Hoeltzle MV, Hagan JB, Park MA, Li JT, Abraham RS . Phenotypic andclinical heterogeneity associated with monoallelic TNFRSF13B-A181E mutations incommon variable immunodeficiency. Hum Immunol. 2010 May; 71(5):505-11. Epub 2010Mar 12. PMID:20156508. DOI:10.1016/j.humimm.2010.02.002.

19.

Zuckerman NS, McCann KJ, Ottensmeier CH, Barak M, Shahaf G, Edelman H,Dunn-Walters D, Abraham RS , Stevenson FK, Mehr R. Ig gene diversification andselection in follicular lymphoma, diffuse large B cell lymphoma and primary centralnervous system lymphoma revealed by lineage tree and mutation analyses. Int Immunol.2010; 22(11):863-73.

20.

Cummins NW, Deziel PJ, Abraham RS , Razonable RR. Deficiency of cytomegalovirus(CMV)-specific CD8+ T cells in patients presenting with late-onset CMV disease severalyears after transplantation. Transpl Infect Dis. 2009 Feb; 11(1):20-7. Epub 2008 Sep 21. PMID:18811629. DOI:10.1111/j.1399-3062.2008.00344.x.

21.

* Joshi AY, Iyer VN, Boyce TG, Hagan JB, Park MA, Abraham RS . Elevated serumimmunoglobulin E (IgE): when to suspect hyper-IgE syndrome-A 10-year pediatric tertiarycare center experience. Allergy Asthma Proc. 2009 Jan-Feb; 30(1):23-7. PMID:19331717.DOI:10.2500/aap.2009.30.3193.

22.

* Park MA, Li JT, Hagan JB, Maddox DE, Abraham RS . Common variable23.



immunodeficiency: a new look at an old disease. Lancet. 2008 Aug 9;372(9637):489-502. PMID:18692715. DOI:10.1016/S0140-6736(08)61199-X.

Shepard BD, De Forni D, McNamara DR, Foli A, Rizza SA, Abraham RS , Knutson K,Wettstein PJ, Lori F, Badley AD. Beneficial effect of TRAIL on HIV burden, withoutdetectable immune consequences. PLoS One. 2008; 3(8):e3096. Epub 2008 Aug 28.PMID:18769477. PMCID:2517653. DOI:10.1371/journal.pone.0003096.

24.

* Martin W, Abraham R , Shanafelt T, Clark RJ, Bone N, Geyer SM, Katzmann JA,Bradwell A, Kay NE, Witzig TE. Serum-free light chain-a new biomarker for patients withB-cell non-Hodgkin lymphoma and chronic lymphocytic leukemia. Transl Res. 2007 Apr;149(4):231-5. PMID:17383597.

25.

Abraham RS , Manske MK, Zuckerman NS, Sohni A, Edelman H, Shahaf G, Timm MM,Dispenzieri A, Gertz MA, Mehr R. Novel analysis of clonal diversification in blood B celland bone marrow plasma cell clones in immunoglobulin light chain amyloidosis. J ClinImmunol. 2007 Jan; 27(1):69-87. Epub 2006 Dec 28. PMID:17192818.DOI:10.1007/s10875-006-9056-9.

26.

Abraham RS , Manske MK, Sohni A, Zuckerman NS, Edelman H, Shahaf G, Timm MM,Dispenzieri A, Gertz MA, Mehr R. Graphical quantification of B cell lineage trees revealsclonal diversification in immunoglobulin light chain amyloidosis. J Clin Immun. 2007;27(1):69-87.

27.

Steiman-Shimony A, Edelman H, Hutzler A, Barak M, Zuckerman NS, Shahaf G,Dunn-Walters D, Stott DI, Abraham RS , Mehr R. Lineage tree analysis ofimmunoglobulin variable-region gene mutations in autoimmune diseases: chronicactivation, normal selection. Cell Immunol. 2006 Dec; 244(2):130-6. Epub 2007 Apr 16. PMID:17434468. DOI:10.1016/j.cellimm.2007.01.009.

28.

Katzmann JA, Dispenzieri A, Kyle RA, Snyder MR, Plevak MF, Larson DR, Abraham RS, Lust JA, Melton LJ 3rd, Rajkumar SV. Elimination of the need for urine studies in thescreening algorithm for monoclonal gammopathies by using serum immunofixation andfree light chain assays. Mayo Clin Proc. 2006 Dec; 81(12):1575-8. PMID:17165636.DOI:10.4065/81.12.1575.

29.

* Manske MK, Zuckerman NS, Timm MM, Maiden S, Edelman H, Shahaf G, Barak M,Dispenzieri A, Gertz MA, Mehr R, Abraham RS . Quantitative analysis of clonal bonemarrow CD19+ B cells: use of B cell lineage trees to delineate their role in thepathogenesis of light chain amyloidosis. Clin Immunol. 2006 Jul; 120(1):106-20. Epub2006 Mar 03. PMID:16515886. DOI:10.1016/j.clim.2006.01.008.

30.

Dispenzieri A, Lacy MQ, Katzmann JA, Rajkumar SV, Abraham RS , Hayman SR,Kumar SK, Clark R, Kyle RA, Litzow MR, Inwards DJ, Ansell SM, Micallef IM, Porrata LF,Elliott MA, Johnston PB, Greipp PR, Witzig TE, Zeldenrust SR, Russell SJ, Gastineau D,Gertz MA. Absolute values of immunoglobulin free light chains are prognostic in patientswith primary systemic amyloidosis undergoing peripheral blood stem cell transplantation.Blood. 2006 Apr 15; 107(8):3378-83. Epub 2006 Jan 05. PMID:16397135. PMCID:1895763.DOI:10.1182/blood-2005-07-2922.

31.

Steiman-Shimony A, Edelman H, Barak M, Shahaf G, Dunn-Walters D, Stott DI, Abraham RS , Mehr R. Immunoglobulin variable-region gene mutational lineage treeanalysis: application to autoimmune diseases. Journal of Autoimmunity Reviews. 2006;5:242-251.

32.

Katzmann JA, Abraham RS , Dispenzieri A, Lust JA, Kyle RA. Diagnostic performance ofquantitative kappa and lambda free light chain assays in clinical practice. Clin Chem.2005 May; 51(5):878-81. PMID:15774572.

33.

Abraham RS , Ballman KV, Dispenzieri A, Grill DE, Manske MK, Price-Troska TL, PazNG, Gertz MA, Fonseca R. Functional gene expression analysis of clonal plasma cellsidentifies a unique molecular profile for light chain amyloidosis. Blood. 2005 Jan 15;

34.



105(2):794-803. PMID:15388584.

Abraham RS , Geyer SM, Ramirez-Alvarado M, Price-Troska TL, Gertz MA, Fonseca R.Analysis of somatic hypermutation and antigenic selection in the clonal B cell inimmunoglobulin light chain amyloidosis (AL). J Clin Immunol. 2004 Jul; 24(4):340-53. PMID:15163890.

35.

Bergen HR, Abraham RS , Johnson KL, Bradwell AR, Naylor S. Characterization ofamyloidogenic immunoglobulin light chains directly from serum by on-line immunoaffinityisolation. Biomed Chromatogr. 2004 Apr; 18(3):191-201. PMID:15103706.

36.

Abraham RS , Geyer SM, Price-Troska TL, Allmer C, Kyle RA, Gertz MA, Fonseca R.Immunoglobulin light chain variable (V) region genes influence clinical presentation andoutcome in light chain-associated amyloidosis (AL). Blood. 2003 May 15;101(10):3801-8. PMID:12515719.

37.

Abraham RS , Katzmann JA, Clark RJ, Bradwell AR, Kyle RA, Gertz MA. Quantitativeanalysis of serum free light chains - A new marker for the diagnostic evaluation of primarysystemic amyloidosis. Am J Clin Pathol. 2003 Feb; 119(2):274-8. PMID:12579999.

38.

Kudva YC, Rajagopalan G, Raju R, Abraham RS , Smart M, Hanson J, David CS.Modulation of insulitis and type 1 diabetes by transgenic HLA-DR3 and DQ8 in NODmice lacking endogenous MHC class II. Hum Immunol. 2002 Nov; 63(11):987-99. PMID:12392851.

39.

Abraham RS , Charlesworth MC, Owen BAL, Benson LM, Katzmann JA, Reeder CB,Kyle RA. Trimolecular complexes of lambda light chain dimers in serum of a patient withmultiple myeloma. Clin Chem. 2002 Oct; 48(10):1805-11. PMID:12324506.

40.

Katzmann JA, Clark RJ, Abraham RS , Bryant S, Lymp JF, Bradwell AR, Kyle RA.Serum reference intervals and diagnostic ranges for free kappa and free lambdaimmunoglobulin light chains: relative sensitivity for detection of monoclonal light chains.Clin Chem. 2002 Sep; 48(9):1437-44. PMID:12194920.

41.

Abraham RS , Clark RJ, Bryant SC, Lymp JF, Larson T, Kyle RA, Katzmann JA.Correlation of serum immunoglobulin free light chain quantification with urinary BenceJones protein in light chain myeloma. Clin Chem. 2002; 48(4):655-7. PMID:11901068.

42.

Naumov YN, Bahjat KS, Gausling R, Abraham R , Exley MA, Koezuka Y, Balk SB,Strominger JL, Clare-Salzer M, Wilson SB. Activation of CD1d-restricted T cells protectsNOD mice from developing diabetes by regulating dendritic cell subsets. Proc Natl AcadSci U S A. 2001 Nov 20; 98(24):13838-43. Epub 2001 Nov 13. PMID:11707602. PMCID:61128.DOI:10.1073/pnas.251531798.

43.

Kudva YC, Deng YJ, Govindarajan R, Abraham RS , Marietta EV, Notkins AL, David CS.HLA-DQ8 transgenic and NOD mice recognize different epitopes within the cytoplasmicregion of the tyrosine phosphatase-like molecule, IA-2. Hum Immunol. 2001 Oct;62(10):1099-105. PMID:11600216.

44.

Abraham RS , Wen L, Marietta EV, David CS. Type 1 diabetes-predisposing MHCalleles influence the selection of glutamic acid decarboxylase (GAD) 65-specific T cells ina transgenic model. J Immunol. 2001 Jan 15; 166(2):1370-9. PMID:11145722.

45.

Abraham RS , Kudva YC, Wilson SB, Strominger JL, David CS. Co-expression of HLADR3 and DQ8 results in the development of spontaneous insulitis and loss of tolerance toGAD65 in transgenic mice. Diabetes. 2000 Apr; 49(4):548-54. PMID:10871191.

46.

Abraham RS , David CS. Identification of HLA-class-II-restricted epitopes ofautoantigens in transgenic mice. Curr Opin Immunol. 2000 Feb; 12(1):122-9. PMID:10679410.

47.



Das P, Abraham R , David C. HLA transgenic mice as models of human autoimmunediseases. Rev Immunogenet. 2000; 2(1):105-14. PMID:11324683.

48.

Abraham RS , Wilson SB, de Souza NF Jr, Strominger JL, Munn SR, David CS. NODbackground genes influence T cell responses to GAD 65 in HLA-DQ8 transgenic mice.Hum Immunol. 1999 Jul; 60(7):583-90. PMID:10426275.

49.

Das P, Abraham RS , David CS. HLA transgenic mice as models of human autoimmunediseases. Reviews in Immunogenetics. 1999 2; 2:105-11.

50.

Abraham RS , Kudva YC, Wilson SB, Strominger JL, David CS. NOD background genesinfluence T cell responses to GAD 65 in HLA-DQ8 transgenic mice. Diabetes. 1999;49:548-55.

51.

Singh N, Bhatia S, Abraham RS , Basu SK, George A, Bal V, Rath S. Modulation of Tcell cytokine profiles and peptide-MHC complex availability in vivo by delivery toscavenger receptors via antigen maleylation. Journal of Immunology. 1998;160:4869-880.

52.

Abraham RS , Choudhury A, Basu SK, Bal V, Rath S. Disruption of T cell tolerance bydirecting a self-antigen to macrophage-specific scavenger receptor. Journal ofImmunology. 1997; 158:4029-35.

53.

Abraham RS , Singh N, Mukhopadhyay A, Basu S K, Bal V, Rath, S. Modulation ofimmunogenicity and antigenicity of proteins by maleylation to target scavenger receptorson macrophages. Journal of Immunology. 1995; 154:1-8.

54.

Books

Chang A, Husain AN, Gordon IO, Ranganathan S, Cornell LD, Farris AB III, Pogoriler J,Seshan SV, Pai RK, Gandhi MJ, Westerhoff M, Ahn J, Hart J, Wang HL, Yerian L,Ananthanarayanan V, Abraham RS , Kambham N, Bracamonte ER, Meehan SM,Langman LJ, Colvin RB, Akki A, Cendales L, Yeh MM. Diagnostic pathology. Transplantpathology. First Edition. Salt Lake City: Amirsys; 2014. (Book)

1.

Book Chapters

Abraham RS , Barnidge D. Protein Evaluation. In: Manual of Molecular and ClinicalLaboratory Immunology, 8th ed. Washington DC: ASM Press; 2014. (Book chapter)

1.

Abraham RS . Lymphocyte Activation. In: Manual of Molecular and Clinical andLaboratory Immunology. 8th ed. Washington DC: ASM Press; 2014. (Book chapter)

2.

Abraham RS . NK cells. In: Chang A; et al editor. Diagnostic pathology. Transplantpathology. First Edition. Salt Lake City: Amirsys; 2014. p. 1-12-1-17. (Book chapter)

3.

Abraham RS . Laboratory-based immune monitoring in organ transplantation. In: ChangA; et al editor. Diagnostic pathology. Transplant pathology. First Edition. Salt Lake City:Amirsys; 2014. p. 1-18-1-25. (Book chapter)

4.

Abraham RS . Immune response in organ transplantation. In: Chang A; et al editor.Diagnostic pathology. Transplant pathology. First Edition. Salt Lake City: Amirsys; 2014.p. 1-2-1-5. (Book chapter)

5.

Abraham RS . Regulatory immune cells and transplant tolerance. In: Chang A; et aleditor. Diagnostic pathology. Transplant pathology. First Edition. Salt Lake City: Amirsys;2014. p. 1-6-1-11. (Book chapter)

6.

Abraham RS . Immune Response in Organ Transplantation. In: Chang, A et al.

7.



Transplant Pathology Amirsys; 2013. (Book chapter)

Abraham RS . Laboratory-based immune monitoring in Organ Transplantation. In:Chang, A et al. Transplant Pathology. Amirsys; 2013. (Book chapter)

8.

Abraham RS . NK cells in Solid-organ Transplantation (SOT. In: Chang, A et al.Transplant Pathology. Amirsys; 2013. (Book chapter)

9.

Abraham RS . Regulatory Immune cells and Transplant Tolerance. In: Chang A et al,Eds. Transplant Pathology Amirsys; 2013. (Book chapter)

10.

Abraham RS , Barnidge DR, Lanza IR. Assessment of proteins of the immune system.In: Rich RR; et al, editor. Clinical immunology: principles and practice. 4th Edition.Oxford: Elsevier/Saunders; 2013. p. 1145-59. (Book chapter)

11.

Abraham RS , Manske M, Ballman K, Dispenzieri A, Price-Troska T, Gertz MA, FonsecaR. Molecular classification of light chain (AL) amyloidosis by gene expression profiling. In:G Grateau, RA Kyle, M Skinner. Amyloid and Amyloidosis. CRC Press, New York; 2005.p. 58-60. (Book chapter)

12.

Abraham RS , Manske MK, Sohni A, Edelman H, Zuckerman N, Shahaf G, DispenzieriA, Lacy MQ, Mehr R. Analysis of B cell clonal evalution in BM and PBL of AL and MMpatients using graphical quantification of linage trees. In: G Grateau, RA Kyle, M Skinner.Amyloid and Amyloidosis. CRC Press, New York.; 2005. p. 61-63. (Book chapter)

13.

Letters

Abraham RS , Recher M, Giliani S, Walter JE, Lee YN, Frugoni F, Maddox DE, KirmaniS, Notarangelo LD. Adult-onset manifestation of idiopathic T-cell lymphopenia due to aheterozygous RAG1 mutation. J Allergy Clin Immunol. 2013 May; 131(5):1421-3. Epub2012 Nov 02. PMID:23122631. PMCID:3725988. DOI:10.1016/j.jaci.2012.09.016.

1.

Gustafson MP, Abraham RS , Lin Y, Wu W, Gastineau DA, Zent CS, Dietz AB.Association of an increased frequency of CD14+ HLA-DR lo/neg monocytes withdecreased time to progression in chronic lymphocytic leukaemia (CLL). Br J Haematol.2012 Mar; 156(5):674-6. Epub 2011 Nov 3 PMID:22050346. PMCID:3433277.DOI:10.1111/j.1365-2141.2011.08902.x.

2.

Bauer PR, Kashyap R, Abraham RS , Peikert T, Gajic O. Accuracy of waste bloodmeasurement in critically ill patients. Intensive Care Med. 2011 Apr; 37(4):721-2. Epub2011 Jan 18. PMID:21243335. DOI:10.1007/s00134-010-2118-8.

3.

Kumar N, Hagan JB, Abraham RS , Aksamit AJ. Common variableimmunodeficiency-associated myelitis: report of treatment with infliximab. J Neurol. 2008Nov; 255(11):1821-4. Epub 2008 Jul 21. PMID:18677641. DOI:10.1007/s00415-008-0898-3.

4.

* Park MA, Li JT, Hagan JB, Maddox DE, Abraham RS . Primary immunodeficiency: acall for multidisciplinary care Reply. Lancet. 2008 Nov-Dec; 372(9653):1878.

5.

* Jaleel A, Owen BA, Manske MK, Katzmann JA, Kyle RA, Abraham RS . Bence Jonescryoglobulinuria: characterization of a urinary kappa light chain cryoglobulin. Clin Chem.2006 Jul; 52(7):1435-6. PMID:16798972. DOI:10.1373/clinchem.2006.066753.

6.

Abstracts

Walter JE, Rosen LB, Chen K, Lee YN, Tirosh I, Csomos K, Ujhazi B, Al-Herz W, CowanMJ, Puck J, Bleesing JJ, Malech H, DeRavin SS, Gennery AR, Abraham RS , Butte MJ,Nadeau KC, Sullivan KE, Giliani S, Niehues T, Schuetz C, Kuijpers T, Holland SM,Browne SK, Notarangelo LD. Distinct anti-cytokine autoantibody profile is likely driven byinnate hyperinflammation among patients with primary immunodeficiency secondary to

1.



rag mutations. J Clin Immunol. 2014 Apr; 34(3):353-4.

* Pozos TC, Abraham RS , Smith M, Leidal K, Hsu A, Bagatikar J, McCormick S, HindeS, Austin A, Dinauer M, Holland SM, Nauseef W. Novel clinical presentation of completemyeloperoxidase (mpo) deficiency: should mpo deficiency be re-instated as a primaryimmunodeficiency? J Clin Immunol. 2014 Apr; 34(3):369.

2.

Kuhlen JL Jr, Giallourakis CP, Gibbons F, Hesterberg P, Murali M, Abraham RS , WalterJE. A case of autoimmunity in combined immunodeficiency. is this beyond commonvariable immunodeficiency (cvid)? J Clin Immunol. 2014 Apr; 34(3):371.

3.

Kochuparambil S, Jackson A, Litzow MR, Khan SP, Rodriguez V, Abraham R , HoganW, Patnaik M. Allogeneic transplantation for mds/aml patients with germline gata2mutations. Biol Blood Marrow Transplant. 2014 Feb; 20(2):S236-7.

4.

Pyle R, Hagan JB, Martinez A, Joshi AY, Boyce T, Volcheck GW, Yilmaz-Demirdag Y,Bahna SL, Abraham RS . Clinical, immunological and genetic spectrum of novel btkgene mutations in x-linked agammaglobulinemia patients and female carriers. J AllergyClin Immunol. 2014 Feb; 133(2):AB8.

5.

* Bidadi B, May S, Boyce T, Joshi A, Kirmani S, Khan S, Rodriguez V, Kobrinsky N, de laMorena M, Abraham R . Role of immunological and genetic testing in directing themanagement of X-linked thrombocytopenia and Wiskott-Aldrich Syndrome. PrimaryImmunodeficiency Treatment Consortium meeting. 2014.

6.

League SC, Smith M, Martinez A, Jenkins SM, Abraham RS . Validation of alaboratory-developed multi-parametric flow cytometry assay for differentiation betweeninfectious and non-infectious systemic inflammatory response syndrome in critically illpatients. Cytometry B Clin Cytom. 2013 Nov; 84(6):413.

7.

Dietz A, Gustafson MP, Lin Y, LaPlant BR, Liwski CJ, Maas ML, League SC, Bauer PR,Abraham RS , Tollefson MK, Kwon ED, Gastineau DA. Immune monitoring using thepredictive power of immune profiles. Cytotherapy. 2013 Apr; 15(4):S48.

8.

* Samant SA, Joshi AY, Abraham RS . Abnormal neutrophil oxidative burst (noxb) in a72-year old male with secondary hemophagocytic lymphohistiocytosis (hlh). J ClinImmunol. 2013 Apr; 33(3):702.

9.

Tschumper RC, Dispenzieri A, Abraham RS , Henderson KJ, Jelinek DF. Molecularinterrogation of biclonal multiple myeloma for clonal relatedness. Blood. 2012 Nov 16;120(21).

10.

Abraham RS , Dong X, Eckloff B, Middha S, Bram R. Whole-exome sequencing foridentification of a potential novel defect in a patient with rapid-onset fatal virus-associatedhemophagocytic syndrome (VAHS). J Clin Immunol. 2012 Sep; 32:38.

11.

Walter JE, Matthew D, Lee YN, Recher M, Patrizi L, Al-Herz W, Cowan M, Puck J,Bleesing J, Filipovich L, Niehues T, Schuetz C, Drucker G, Malech H, De Ravin SS, UzelG, Facchetti F, Gennery A, Alenezi HM, Chinen J, Dbaibo G, El Ghazali G, Pasic S, ChenK, Nadeau K, Abraham R , Giliani S, Balboni M, Browne S, Notarangelo LD.Characterization of autoantibody profile among patients with primary immunodeficiencysecondary to rag mutations. J Clin Immunol. 2012 Sep; 32:44.

12.

Abraham RS , Dong XY, Nguyen P, Rodriguez V, Khan S, Boyce TG, Joshi AY, Bram R.Rapid-onset fatal EBV-associated hemophagocytic lymphohistiocytosis (HLH) in a childwith heterozygous A91V mutation in the perforin (PRF1) gene. J Clin Immunol. 2012 Apr;32(2):359.

13.

* League SC, Dong XY, Thapa P, Abraham RS . Improvement in accuracy of clinicallymphocyte subset quantitation by flow cytometry using a 7-color assay incorporating

14.



CD14. Cytometry B Clin Cytom. 2011 Nov; 80B(6):384.

* Dong XY, Abraham RS . Clinical utility of a quantitative and functional flow cytometryassay for CMV-specific CD8 T cell immunity in transplant patients. Transpl Int. 2011 Sep;24(2):276.

15.

Juhn Y, Yun H, Urm S, Fenta Y, Yoo K, Abraham R , Hagan J. Assessment of theassociation of asthma status with selective IgA deficiency and common variableimmunodeficiency: a population-based case-control study. J Allergy Clin Immunol. 2011Feb; 127(S):AB153.

16.

Abraham RS , Dong X. Analytical and clinical validation of a diagnostic test forevaluation of NK cell subsets and NKT cells. J Allergy Clin Immunol. 2011 Feb;127(S):AB91.

17.

Khanna S, Abraham R , Faubion W. An immuno-regulatory protein profile induced byanti-TNFa therapy in human Crohn's disease. Inflamm Bowel Dis. 2011 Jan; 17(Suppl1):S14-5.

18.

Zent CS, Abraham R , Lin Y, Gustafson M, Wu W, Dietz A. Immune phenotyping andnaive t cells as a predictor of response to therapy in chronic lymphocytic leukemia (CLL).Blood. 2010 Nov 19; 116(21):585-6.

19.

Murray DL, Theis JD, Vrana JA, Ramirez-Alvarado M, Abraham R , Dispenzieri A,Katzmann JA, Dogan A. Proteomic analysis of immunoglobulin light chains (LC) in alamyloidosis shows that the sequence of clonal LC secreted by the neoplastic plasmacells is identical to the LC deposited in the amyloid plaques. Blood. 2010 Nov 19;116(21):796.

20.

Gustafson MP, Lin Y, League SC, Bulur PA, Abraham RS , Vuk-Pavlovic S, GastineauDA, Dietz AB. Loss of HLA-DR expression on CD14+Cells; a common marker ofimmunosuppression in cancer patients. J Immunother. 2010 Oct; 33(8):869-70.

21.

* Block DR, Jenkins SM, League SC, Snyder MR, Abraham RS . Lymphocyte subsetquantitation by flow cytometry: analytical insights for the clinical laboratory. Am J ClinPathol. 2010 Sep; 134(3):38.

22.

Bauer PR, Kashyap R, Trillo CA, Abraham RS , Balsanek JG, Snyder MR, Peikert T,Gajic O. Biomarkers of acute lung injury in fresh and "waste" blood specimens. IntensiveCare Med. 2010 Sep; 36(Suppl 2):1071.

23.

* Park MA, Snyder MR, Smith C, Jenkins S, Ryu E, Therneau T, Hagan JB, Li JT, Abraham RS . New guidelines for interpretation of IgG pneumococcal antibody data:results from a cohort study of healthy adults. Clin Immunol. 2010 May; 135(2):38.

24.

* Joshi A, Abraham R , Boyce T. Is Down syndrome a primary immunodeficiencydisorder? A population-based cohort study. Clin Immunol. 2010 May; 135(2):96.

25.

* Joshi A, Abraham RS , Boyce TG. Immunological analsis in patients with Downssyndrome. Clin Immunol. 2010 May; 135(2):96.

26.

Kudva Y, Rostambeigi N, Erickson J, Abraham R . Immune monitoring shows profoundimmunological anomalies in pancreas after kidney (PAK) transplant recipients. Am JTransplant. 2010 Apr; 10(Sp Iss SI Suppl 4):490.

27.

Rostambeigi N, Bhalla T, Dean P, Prieto M, Cosio F, Abraham R , Kudva Y. SerologicalCMV mismatch in pancreas transplantation poses seven-fold increased risk of CMVinfection. Am J Transplant. 2010 Apr; 10(Sp Iss SI Suppl 4):546.

28.

Dogan A, Theis JD, Gamez JD, Murray DL, Abraham RS , Grogg KL, Katzmann JA,29.



Dispenzieri A, Zeldenrust SR, Ramirez-Alvarado M, Vrana JA. Proteome immunoglobulinlight chain amyloidosis (AL): insights into the pathogenesis. Amyloid. 2010 Apr; 17(Suppl1):54.

Khanna S, Abraham RS , Faubion WA. Anti-TNF alpha therapy results in marginal zoneB-cell up-regulation in human Crohn's disease. Gastroenterology. 2010; 138(5 Suppl1):S-130.

30.

* Bellavia D, Abraham RS , Pellikka PA, Dispenzieri A, Burnett JC, Al-Zahrani GB, GertzMA, Miller FA, Abraham TP. Utility of Doppler myocardial imaging, cardiac biomarkersand clonal immunoglobulin genes to assess left ventricular performance and stratify riskfollowing peripheral blood stem cell transplantation in patients with systemic light chainamyloidosis J Am Coll Cardiol. 2010; 55(10 Suppl A):A24.

31.

* Erickson JR, League SC, Rostambeigi N, Kudva Y, Abraham RS . Analytical andclinical validation of an EDU (R)-based flow cytometry assay to measure lymphocytefunction in the clinical laboratory. Cytometry Part B-clinical Cytometry. 2009 Nov;76B(6):398-9.

32.

Clark RJ, Bryant SC, Abraham RS , Snyder MR, Katzmann JA. Adult and pediatricreference ranges for IgG subclasses. Clin Chem. 2009 Jun; 55(Suppl S):A130-1.

33.

Balsanek JG, Peters BE, Abraham RS , Snyder MR. Analytical validation of abead-based multiplex cytokine assay. Clin Chem. 2009 Jun; 55(Suppl S):A134.

34.

Kudva YC, Rostambeigi N, John S, Mailankody S, Pedersen RA, Larson TS, Prieto M,Cosio FG, Dean PG, Kremers WK, Abraham RS , Stegall MD. Epidemiology ofinfections requiring hospitalization after pancreas transplantation. Am J Transplant. 2009;9(Suppl 2):709.

35.

Yilmaz-Demirdag Y, Dong X, Abraham RS , Bahna SL. Novel splice site mutation in theBTK gene in 2 brothers with hypogammaglobulinemia. Ann Allergy Asthma & Immun.2009; 103(Supp 3):A108.

36.

* Joshi A, Park M, Hagan J, Boyce T, Li J, Reed A, Maddox D, Hartz M, Volcheck G,Rank M, Katzmann J, Snyder M, Abraham R . Decrease in class-switched memory Bcells is associated with malignancy in common variable immunodeficiency (CVID). ClinImmunol. 2009; 131:S136-7.

37.

* Park MA, Li JT, Hagan JB, Maddox DE, Abraham RS . Primary Immunodeficiency: acall for multidisciplinary care. Lancet (Reply). 2009; 55(supp S):A134.

38.

Abraham RS , Dong X, Park MA, Hagan JB, Li JT. Accumulation of specific memoryB-cell subsets in peripheral blood of healthy adults in response to pneumococcalvaccination. Clin Exp Immunol. 2008 Nov; 154(Suppl 1):14-5.

39.

Abraham RS . Identification of CTL Epitopes on BK virus VP1 protein for assessment ofimmune competence in transplant-associated BK nephropathy. J Allergy Clin Immunol.2008 Feb; 121(2 Suppl 1):S170.

40.

* Dong XY, Hoeltzle M, Nandakumar V, Dunker J, Ansell S, Bram R, Hagan J, Park M, Abraham R . Evaluation of the frequency and phenotypic heterogeneity of theTNFRSF13B (TACI) A181E mutation in a cohort of CVID patients. Clin Immunol. 2008;127(Suppl S):S65.

41.

* Bellavia D, Abraham RS , Pellikka PA, Dispenzieri A, Oh JK, Al-Zaharani GB, Green T,Miller FA, Abraham TP. The influence of immunoglobulin gene usage on myocardialmechanics and mortality in systemic light chain amyloidosis (AL). J Am SocEchocardiogr. 2008; 21:529.

42.



* Snyder M, Matteson EL, Abraham RS , Homburger HA. Lymphocyte tyrosinephosphatase PTPN22 genotype enhances the specificity of cyclic citrullinated peptideantibodies for rheumatoid arthritis (Abstract C-141). Clin Chem. 2007 Jun; 53(6 SupplS):A147.

43.

* Chenworth EC, Nandakumar V, Abraham RS . Development of a clinical flowcytometry assay to measure in vitro lymphocyte responses. J Allergy Clin Immunol. 2007Jan; 119(1 Suppl 1):S98.

44.

Hagan JB, Abraham RS , Park MA, Volcheck GW, Maddox DE, Butterfield JH, Li JTC.Why are patients referred to a non HIV adult immunodeficiency clinic? J Allergy ClinImmunol. 2007 Jan; 119(1 Suppl S):S251.

45.

Katzmann JA, Abraham RS , Dispenzieri A, Kyle RA, Lust JA, Snyder MR, Plevak MF,Larson DR, Melton LJ, Rajkumar SV. Serum immunofixation and free light chain assayseliminate the need for urine studies during diagnostic work up of monoclonalgammopathies. Amyloid. 2006 Nov; 13(Suppl 1):36.

46.

Katzmann JA, Dispenzieri A, Kyle R, Snyder MR, Plevak MF, Larson DR, Abraham RS ,Lust JA, Melton LJ, Rajkumar SV. Elimination of the need for urine studies duringdiagnostic studies of monoclonal gammopathies by the combined use of a serumimmunofixation and serum free light chain assays (Abstract 5011). Blood. 2006 Nov;108(11 part 2):341b.

47.

Dispenzieri A, Lacy MQ, Katzmann JA, Rajkumar SV, Abraham RS , Hayman SR,Kumar SK, Clark R, Kyle RA, Litzow MR, Inwards DJ, Elliott MA, Micallef IV, Ansell SM,Porrata LF, Johnston P, Zeldenrust SR, Witzig TE, Greipp PR, Lust JA, Russell SR, GertzMA. Absolute values of serum immunoglobulin free light chains predict for survival inpatients with primary systemic amyloidosis undergoing peripheral blood stem celltransplant (Abstract 422). Blood. 2005 Nov; 106(11):127a.

48.

Katzmann JA, Dispenzieri A, Abraham RS , Lust JA, Kyle RA. Diagnostic performance offree light chain assays in clinical practice (Abstract D-16). Clin Chem. 2005; 51(6Suppl):A161.

49.

* Manske MK, Maiden S, Abraham RS . Clonal B cells in blood and bone marrow ofpatients with a plasma cell dyscrasia- light chain amyloidosis (Abstract Sa2.113). ClinImmunol. 2005; (Suppl 1):S179-S180.

50.

* Henon K, Dispenzieri A, Katzmann J, Lacy M, Ramirez-Alvarado, Gertz M, Kyle R, Abraham R . Circulating soluble light chain oligomers in sera of patients with light chainamyloidosis. (Abstract P0.1406). Haematologica. 2005; 90(S1):200.

51.

Katzmann JA, Dispenzieri A, Abraham RS , Kyle RA. Performance of free light chainassays in clinical practice. Blood. 2004 Nov 16; 104(11 Part 1):216A-7A.

52.

Abraham RS , Manske MK, Sohni A, Edelman H, Zuckerman N, Shahaf G, DispenzieriA, Lacy MQ, Gertz MA, Mehr R. Analysis of B cell clonal evolution in BM and PBLsequences of AL and MM patients using graphical quantification of lineage trees.Proceedings of the Xth INternational Symposium on Amyloid and Amyloidosis ToursFrance. 2004 Apr 19.

53.

Dispenzieri A, Lacy MQ, Therneau T, Larson D, Rajkumar SV, Fonseca R, Greipp PR,Lust JA, Zeldenrust S, Snow D, Abraham R , Plevak M, Gertz MA. Case control studysuggests that survival is superior in amyloid patients undergoing peripheral blood stemcell transplant. Blood. 2003 Nov 16; 102(11):118a.

54.

* Martin W, Clark RJ, Shanafelt T, Katzmann JA, Bradwell A, Abraham R , Kay NE,Witzig TE. Detection of serum free light chains in patients with B-cell non-Hodgkinlymphoma (NHL) and chronic lymphocytic leukemia (CLL). Blood. 2003 Nov 16;102(11):276b-7b.

55.



* Manske MK, Gonzalez-Paz NC, Ballman KV, Dispenzieri A, Price-Troska TL, FonsecaR, Abraham RS . Gene expression profiling of clonal plasma cells in light chain (AL)amyloidosis by microarray analysis. Blood. 2003 Nov 16; 102(11):369a.

56.

Abraham RS , Geyer SM, Ramirez-Alvarado M, Price-Troska TL, Allmer C, Kyle RA,Gertz MA, Fonseca R. Immunoglobulin variable (V) region germline genes andmutational hotspots potentially influence protein misfolding in light chain amyloidosis (AL)(Abstract 2375). Blood. 2002 Nov 16; 100(11):603a-604a.

57.

Katzmann JA, Abraham RS , Clark RC, Kyle RA, Gertz MA. Utility of free light chainquantitation in assessment of response following autologous stem cell transplantation forprimary amyloidosis. Clin Chem. 2002 Jun; 48(6 Suppl):A163.

58.

Abraham RS , Price-Troska TL, Allmer C, Geyer SM, Kyle RA, Gertz MA, Fonseca R.Immunoglobulin light chain variable (V) region gene usage determines organ tropism andoverall survival in light chain-associated (AL) amyloidosis. FASEB J. 2002 Mar 20;16(4):A351.

59.

Kyle RA, Gertz MA, Katzmann JA, Clark RJ, Abraham RS . Utility of serum free lightchain measurements for assessing response following autologous stem celltransplantation for primary amyloidosis (AL). (Abstract 4.2.4.). Amyloid. 2001 Dec;8(Suppl. 2):71-2.

60.

Naylor S, Abraham RS , Bradwell AR, Fonseca R, Katzmann J, Kyle RA, Bergen HR III.Analysis of immunoglobulin light chains in primary amyloidosis--direct on-line assay foramyloidogenic kappa and lambda light chains directly from serum. (Abstract 4.3.6.).Amyloid. 2001 Dec; 8(Suppl. 2):86.

61.

Abraham RS , Price-Troska TL, Gertz MA, Kyle RA, Fonseca R. Association ofrearranged light chain variable (V) region genes with organ tropism in lightchain-associated (AL) amyloidosis. Blood. 2001 Nov 16; 98(11 Part 2):151b.

62.

Abraham RS , Bergen HR, Naylor S, Katzmann JA, Bradwell AR, Kyle RA, Fonseca R.Characterization of free immunoglobulin light chains (LC) by mass spectrometry in lightchain-associated (AL) amyloidosis. Blood. 2001 Nov 16; 98(11 Part 2):31b.

63.

Abraham RS , Katzmann JA, Clark RJ, Dispenzieri A, Lust J, Bradwell AR, Kyle RA.Analysis of serum immunoglobulin free light chains in primary systemic amylaoidosis andlight chain deposition disease by nephelometry. Amyloid and Amyloidosis. 2001Jul:503-505.

64.

Clark RJ, Katzmann JA, Abraham RS , Lymp JF, Kyle RA, Bradwell AR. Detection ofmonoclonal free light chains by nephelometry: Normal ranges and relative sensitivity. ClinChem. 2001 Jun; 47(S6):A27.

65.

Abraham RS , Katzmann JA, Clark RJ, Dispenzieri A, Lust JA, Bradwell AR, Kyle RA.Detection of serum immunoglobulin free light chains in primary amyloidosis and lightchain deposition disease by nephelometry. Clin Chem. 2001 Jun; 47(S6):A32-A33.

66.

Abraham RS , Katzmann JA, Clark RJ, Lymp JF, Dispenzieri A, Lust JA, Bradwell AR,Kyle RA. Light chain myeloma: Correlation of serum nephelometric analysis for thequantitation of immunoglobulin free light chain with urine Bence Jones protein. ClinChem. 2001 Jun; 47(S6):A33.

67.

Abraham RS , Wen L, David CS. Spontaneous T cell autoreactivity to rat GAD65 in HLAtransgenic mice with Type 1 diabetes-susceptible MHC haplotypes but not withdiabetes-resistant haplotypes. Diabetes. 2000 May; 49(suppl 1):A69.

68.

Kudva YC, Abraham RS , David CS. Modulation of insulitis and type 1 diabetes mellitus69.



in NOD mice lacking MHC class II genes in the presence or absence of HLA DR3. 60thScientific Sessions, American Diabetes Association, San Antonio. 2000.

Abraham RS , Kudva YC, David CS. Type I diabetes-susceptible and resistant HLAClass II molecules modulate insulitis in transgenic mice. Diabetes. 1999 May;48(suppl):A209.

70.

Kudva YC, Abraham RS , David CS. Identification of HLA DR3 restricted GAD 65epitopes in transgenic mice. Diabetes 48 (supplement 1): 39A, 1999.

71.

Abraham RS , David CS. Characterization and kinetic analysis of spontaneous insulitisin HLA DR3/DQ8 transgenic mice. Hum Immunol. 1999; 60(Suppl 2):S87.

72.

Abraham RS , Wilson SB, Strominger JL, Munn SR, Munn SR, David CS. T cellresponses to GAD 65 in NOD/D!8/A beta knock-out transgenic mice: role of backgroundgenes. Diabetes. 1998 May (suppl); 47:232A.

73.

Abraham RS , Bradley DS, Deshmukh U, Fu SM, Luthra HS, David CS. T and B cellepitope mapping of human ro60 in HLA-DR2 and DR3 transgenic mice. (Abstract 1080).Arthritis Rheum. 1997 Sep; 40(9 Suppl):S211.

74.

Miscellaneous

Abraham RS , JT. Book review for JAMA: Handbook of Human Immunology Eds:O'Gorman M and Donnenberg ACRC Press, Taylor and Francis Group Boca Raton, FLHandbook of Human Immunology. 2008. (Book review)

1.

* Indicates that the primary author was a mentee of this author.



Documents

Curriculum Vitae and Bibliography Roshini S. … University, India - BSc, Chemistry and Biology 1983 - 1986 Bangalore University, India - MSc, Microbiology 1986 - 1988 National Institute