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CURRICULUM VITAE BIOGRAPHICAL: Name: Mark S. Kuhlenschmidt Birthplace: Evansville, Indiana Citizenship: USA Marital Status: Married, Theresa Banet Kuhlenschmidt Two children, Matthew Scott and Michael Stuart Business Address: Department of Pathobiology College of Veterinary Medicine University of Illinois 2840 VMBSB 2001 South Lincoln Avenue Urbana, Illinois 61801 Telephone: (217) 333-9039 Home Address: 1406 Cobblefield Rd Champaign, Illinois 61821 Telephone: (217) 398-1270 EDUCATION AND TRAINING: Undergraduate: 1965-1969 Purdue University, West Lafayette, IN BS (Biology-Chemistry) Graduate: 1969-1971 University of Louisville, School of MS (Biochemistry) Medicine, Dept. Biochemistry, Louisville, KY 1971-1975 University of Pittsburgh, School of PhD (Biochemistry) Medicine, Dept. Biochemistry, Pittsburgh, PA Thesis for MS: Glycoprotein Synthesis During Mitochondria Biogenesis in the Germinating Castor Bean. Dissertation for PhD: The Structure and Metabolism of Normal and Variant Forms of Human Serum Alpha-1-Antitrypsin. APPOINTMENTS AND POSITIONS: Academic: 1975-1977 The Johns Hopkins University, Department Postdoctoral Fellow

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of Biology and the McCollum-Pratt Institute, Baltimore, MD 1977-1984 The Johns Hopkins University, Department Associate Research of Biology and the McCollum-Pratt Scientist Institute, Baltimore, MD 1984-1990 University of Illinois, College of Assistant Professor Veterinary Medicine, Department of of Microbiology Pathobiology, Urbana, IL 1990-1999 University of Illinois, College of Associate Professor Veterinary Medicine, Department of of Microbiology Pathobiology, Urbana, IL 1999-present University of Illinois, College of Professor of Microbiology Veterinary Medicine, Department of Pathobiology, Urbana, IL 2000-2011 University of Illinois, College of Professor of Microbiology Veterinary Medicine, Department of Assistant Department Head Pathobiology, Urbana, IL 2003-present University of Illinois, College of ACES, Professor of Microbiology Department of Agricultural and Biological Department Affiliate Engineering, Urbana, IL 2004-present University of Illinois, College of ACES, Professor of Microbiology Division of Nutritional Sciences Urbana, IL 2004-2008 University of Illinois, Office Vice Chancellor Director, IACUC Program for Research 2011 - University of Illinois, College of Professor of Microbiology Veterinary Medicine, Department of Interim Department Head Pathobiology, Urbana, IL HONORS AND AWARDS NIH-USPHS Predoctoral Fellow Olive V. Levin Memorial Fellow of the Leukemia Society of America Sigma Xi Who's Who in Veterinary Science and Medicine Morris Animal Foundation Award for Outstanding Research Omega Tau Sigma Veterinary Honor Fraternity (Honorary Member - elected 1985) Phi Zeta National Veterinary Honor Society, (Honorary Member - elected April 1993) University of Illinois, College of Veterinary Medicine Research Award -1994 University of Illinois Incomplete List of Teachers Ranked Excellent by their students –1995-1999 University of Illinois College of Veterinary Medicine Nomination for All-Round Excellence-1996, 1999 Engineering Open House/Agricultural Engineering, First Place Award for Original Research, 2001

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University of Illinois, College of Veterinary Medicine All-Round Excellence Award, 2006 MEMBERSHIPS IN PROFESSIONAL SOCIETIES American Society for Biochemistry and Molecular Biology American Society for Microbiology American Chemical Society Illinois State Veterinary Medical Association (Associate) TEACHING EXPERIENCE: University of Pittsburgh School of Medicine: Teaching Assistant for undergraduate laboratory courses in basic biochemistry and molecular bi-ology. Teaching Assistant responsible for a recitation class in Introductory Organic Chemistry. Teaching Assistant for medical school biochemistry conference sections (recitation classes taught in Journal Club format that were part of the core biochemistry course in the professional curricu-lum. University of Illinois College of Veterinary Medicine: 1983 - Member of the Graduate Faculty of University of Illinois. 1983-1987 - Responsible 1/3 of the 4-credit hour core microbiology course (lecture and laborato-ry) taught to Veterinary Students (PATH 331). This course presents introductory microbiology and biochemistry, molecular mechanisms of disease, and individual pathogenic genera of bacteria, and fungi 1988-1992 - Course Coordinator to above-described course (PATH 331). In 1988 this course be-came eligible to be taught in the graduate curriculum as well as the professional curriculum. 1985-1986 - VP 452. Invited lecturer in graduate course on Tumor Biology of Cell Surfaces 1985-1986 - Coordinator of Microbiology/Immunology Journal Club 1986 - Invited lecturer in graduate biochemistry course, Department of Biochemistry, School of Medicine, University of Illinois at Chicago - Cell-Cell Recognition and Adhesion. 1990-1993 - Chemistry 440/Biochem. 440/Biophys. 440. Invited lecturer in graduate course on biomembranes/cell-cell communication. 1990 - Author and Consultant to Development of “Bacteriology Case Studies” - NovaNet, Univer-sity of Illinois 1992 - 1998 - Responsible 1/2 of the core microbiology lecture and lab course taught to Veteri-nary Students (PATH 331).

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1992 - present - Author and Creator of "HyperBug-DVM: An Interactive, Hypercard Based Elec-tronic Database and Case Simulator for Teaching Diagnostic Veterinary Microbiology" 1993-1994 - Coordinator of Microbiology/Immunology Journal Club 1993 - present - Invited lecturer in graduate course (VP 419) Free Radicals in Biology - College Vet. Med., Dept. Pathobiology 1994 - 2001 - VPD 199 “Biomedical Science in Health and Disease” - a multidisciplinary discovery course for first semester undergraduates at the University of Illinois 1994 - present - Selected as a Visiting Scientist in the American Society for Microbiology spon-sored Miniority Student Science Careers Support Program (MSSCSP). 1994 - Invited lecturer in Departmental Clinical/Basic Science Correlation Conferences. 1994 - Invited lecturer in graduate course (VP 450) Concepts in Pathology-Mechanism of Dis-ease, Spring 1994 - present - Member, Core Faculty - Interdepartmental Toxicologic Pathology Training Pro-gram 1995 - Chemistry 441/Biochem. 441/Biophys. 441. Invited lecturer in graduate course on biomembranes/cell-cell communication. 1995 - Chemistry 440/Biochem. 440/Biophys. 440. Invited lecturer in graduate course on Recep-tors and Signals (topics: Coated-Pits and Receptor-mediated endocytosis; LDL receptor).

1995 -1996 - Co-Designer and implementation of VetNet, an internet program for network presen-tation Clinical Correlation Conferences and VP331 Case studies.

1995 – present - Invited Participant in graduate course (VP 413) on Grant Writing

1997 - 1998 - Participant in the development of VetBac - an international consortium of scientists for development of a web based course on Veterinary Bacteriology. 1998 – 2000 - Course Coordinator for Veterinary Microbiology (VP 331), a core required course in the DVM professional curriculum. In 1988 this course became eligible to be taught in the gradu-ate curriculum as well as the professional curriculum.

2000 – present – Responsible for 1/2 of the lectures and laboratories in Veterinary Microbiology (VP 331), a core required course in the DVM professional curriculum. 2004 – Guest Lecturer, VB 394: Research Development (Elective in DVM Curriculum), Mock IACUC meeting and Proposal Review. 2008 – present – consultant and trainer on laboratory diagnosis of Especially Dangerous Patho-gens (EDP), Defense Threat Reduction Agency (DTRA), Biological Threat Reduction Program (DTRP), Joint University Partnership, Baku, Azerbaijan and Tbilisi, Georgia

2010 – Course Coordinator, Pathobiology 631, Bacteriology and Mycology, fall 2010, 4 credit hours. 2011 – Course Coordinator for VM607 (Pathobiology II, Infectious Diseases), core veterinary cur-riculum (10 credit hours), Spring 2011

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Teaching Support-Grants Awarded

One of several selected faculty collectively awarded a USDA PhD Training Grant, Molecular Mechanisms Of Infectious Diseases , 1986 -1991 Apple Seedlings Program - "HyperBug: An Interactive, Hypercard Based Electronic Microbiology Lab and Case Simulator for Teaching Diagnostic Veterinary Microbiology." PI: Mark S. Kuhlenschmidt, $8,500. Computer Equipment Award (funded 5/90). 1993 - 1994 - U of I Educational Technologies Board (ETB), HyperBug/DVM, Continuation of Support, (1/2 time graduate assistantship, 2 mo. summer, full time), PI: Mark S. Kuhlenschmidt 1994-1995 - Enhanced computer technology to support problem-oriented instruction in veterinary medicine. PI: RD Smith, Co-Investigators: JC Eurell, GL Foley, RA Hess, R. Ott, GD Koritz, DR Krawiec, M. Kuhlenschmidt, RM Weigel, and JF Zachary. Computer Fund Advisory Committee, University of Illinois, $30,000.00 1995-1996 - Educational Technologies Board, University of Illinois, “An Interactive, Problem-Based Electronic Network Module for Veterinary Microbiology and Clinicopathological Confer-ences. PI: Mark S. Kuhlenschmidt, Co-Investigators: JF Zachary, RD Smith. $5850.00 1995-1997 - University of Illinois Learning Technologies in Higher Education Program. “Asyn-chronous Learning Network in Support of a Virutual Veterinary Curriculum”. PI: RD Smith, Co-Investigators: VR Beasley, WH Hascheck, RA Hess, RE Isaacson, MS Kuhlenschmidt, GY Mil-ler, J Messick, RS Ott, VE Valli, RL Wallace, M Williams and JF Zachary. $43,537.00 1996-1997 - Advanced Learning Tecnologies in Higher Education Program, “Grand Rounds on the World Wide Web, PI: RD Smith, Co-Investigators: RB Clarkson, RE Isaacson, MS Kuhlenschmidt, JB Messick, VE Valli, EL Whittem and FE Zuckermann. $41,400.00 1997 - Development of UI-Online Distance Learning Modules for Continuing Education and Certi-fication Programs in Food Safety - Proposal submitted to UI-Online coordinator, Dr. Burks Oakley, CoPIs: MS Kuhlenschmidt and Fred Troutt. 1997 - Partnership to Provide Internet-Based Distance Learning for Illinois Veterinarians, A Part-nership Illinois Seed Grand Proposal, PI: RD Smith, CoI: MS Kuhlenschmidt, RL Wallace (pend-ing) Professional (DVM) Students Research Advising Stephen Hadley, Todd Duffy, Debra Barnes, Rachel Boyce, Karen Crownhart, Salianne Schlacks, Katie Parent, Derrick Hall, Carrie Sherwod, Charlie Deutsch, Johanna Salzer Undergraduate Students Research Advising Tracy Odvody (Hughes Undergraduate Research Fellow) Paul Shao (Colgate-Palmolive Fellow) Dan Bergner (Hughes Undergraduate Research Fellow, American Society Microbiology Fellow)Nathan Vos (Hughes Undergraduate Research Fellow, American Society Microbiology Fellow) Sally Ladsaria (Honors Biology Student – School of Integrative Biology) MS Thesis Advisory Committee Member (Department of Veterinary Pathobiology):

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Sheree Guiffaut Steve Hadley John Urbance

Carrie Arygilon Susanne St. Denis Anisha Misra

Paul Davidson

PhD Dissertation Advisory Committee Member (Department of Pathobiology): Pete Didier Brian Knight Mark Rolsma Julie Johnson Annette Schleuter Dan Schabacker Philip Solter Lois Lawrisuk Chuzhao Lin Michael Cieslewicz Thomas J. Reilly Tetsuya Furuya Nicole Vanderhyden Laura Gumprecht Brain Bailey Robin Sanecki Peter Rohloff Steve Hoosier Michael Okura Hue Duy Kapil Vashisht Namit Ghildyal Joann Schmidt Jean Petter Artem Shkumatov David Coleman Robin Holland Graduate Committees (outside Department of Pathobiology) PhD Advisory for Jian Zhang (Department of Bioacoustics and Biophysics) MS Thesis for Jennifer Trask (Department of Agricultural Engineering) MS Thesis Advisory Committee for Stephen McLaughlin (Ag Engineering) PhD Dissertation Committee for Aileen Andres (Food Science and Human Nutrition) MS Thesis Advisory Committee for Karen Ochonicky (Food Science and Human Nutrition) MS Thesis Advisory Committee for Rose Ann Mathai (Division of Nutritional Sciences) MS Thesis Advisory Committee (Major Advisor) for Jennifer Lee (Division of Nutrition Sciences) MS Dissertation Committee for Paul Davidson (Department of Agricultural and Biological EngineerPing) PhD Dissertation Committee for Paul Davidson (Department of Agricultural and Biological Engi-neering) MS Thesis Committee for Daniel Koch (Department of Agricultural and Biological Engineering) PhD Dissertation Committee for Hannah Holscher (Division of Nutritional Sciences) Thesis/Dissertations Directed (serving as major advisor): James Hosek (MS). “Purification and Characterization of Acid Phosphatases of Eimeria vermiformis”. Currently a practicing veterinarian

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Marian Rippy (PhD- 1989). “A Pathobiochemical Investigation of Glucocorticoid Hepatopathy and Alkaline Phosphatase Isozymes in the Dog”. Currently employed in industry

Thomas Reilly (PhD - 1994). “Identification and Characterization of the Acid Phosphatase from Francisella tularensis and its Potential role in Pathogenesis”. Currently a postdoctoral fellow, University of Maryland School of Medicine. Mark Rolsma (PhD - 1995) “Identification, Isolation and Characterization of an Enterocyte Re-ceptor for Porcine Rotavirus”. Currently an Assistant Professor of Pathology, Auburn University College of Veterinary Medicine Julie Johnson (PhD) “Characterization of an Enterocyte Receptor for Cryptosporidium Parvum (Ph.D awarded 2001) Nathan Markward (PhD) “Molecular Epidemiology of Cryptosporidiosis parvum (started fall 1998) – transferred to Johns Hopkins and then Tulane University JoAnn Schmidt (PhD) – Mechanism of Cryptosporidium parvum Host Cell Interactions (started fall 2000, advanced to candidacy spring 2002) Jennifer Trask (MS) - Transport of Cryptosporidium parvum in Overland and Near-surface Flow, MS degree awarded 2002, Co-advisor with Dr. Prasanta Kalita, Agricultural and Biological Engi-neering Stephen McLaughlin (MS) – Adsorption Kinetics of Cryptosporidium parvum to Soils and Vegeta-tion, MS degree awarded 2003, Co-advisor with Dr. Prasanta Kalita, Agricultural and Biological Engineering Aline Andres (PhD) – (stated spring 2003) – Effects of Soy Isoflavones Intake on Rotavirus Infec-tion Recovery in Piglets, Co-advisor with Dr. Sharon Donovan, Nutritional Sciences Karen Ochonicky (MS) – Efficacy of Porcine Milk Oligosaccharide for the Prevention and Treat-ment of Rotavirus Disease (DVM , MS student, started fall 2003, co-advisor with Dr. Sharon Do-novan, Nutritional Sciences Jennifer Lee (MS) – Long-Chain Polyunsaturated Fatty Acid-Mediated Inhibition of Infectivity of Apicomplexan Parasites. Rose Ann Mathai (MS) – Impact of Soy Components on Intestinal Function in Healthy Piglets Daniel Koch (MS) – Fate and Transport of Cryptosporidium parvum under Small-Scale Rainfall Simulator (Co-major advisor with Dr. Prasanta Kalita, Department of Agricultural and Biological Engineering), 2009. Paul Davidison (PhD) – Characterization of Pathogen Transport in Overland Flow (Co-major ad-visor with Dr. Prasanta Kalita, Department of Agricultural and Biological Engineering), 2010. Rabin Bhattarai (PhD) - Development of a physically-based model for transport of Cryptosporidi-um parvum in overland flow; Department of Agricultural and Biological Engineering Lei Lu (MS) – Effect of Human Milk and Infant Formula-derived Oligosaccharides and Lipids on Human Influenza Virus (H1N1) Infectivity

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Xin Tang (PhD) co-mentor with Professor Taher M. Saif, Department of Mechanical Engineeering and Sciences PostDoctoral Fellows/Visiting Scholars Yao Li (PhD), Visiting Scholar, (in collaboration with Dr. Sharon Donovan, Food Science and Human Nutrition, University of Illinois) Animal Nutrition and Feed Science, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China, People’s Republic of Min Li (PhD), Postdoctoral Fellow, (in collaboration with Dr. Sharon Donovan, Dept. Food Sci-ence and Human Nutrition, Division of Nutritional Sciences, University of Illinois), 2009-2010 Yongzheng Peng (MD), visiting scholar, 2010-2011, First Military Medical University, Zhujiang Hospital, Guangzhou, Guangdong PR, China Bahaa Fadl-Alla (DVM, PhD) 2013- COMMITTEE AND SERVICE ACTIVITIES: DEPARTMENTAL

1984 Inter-departmental Molecular Genetics Search Committee 1984 Mammalian Biotechnology Seminar Committee 1984-present Consultant on purchase and operation of major departmental equipment 1984 -1986 Graduate Admissions Committee 1984 -1987 Capricious Grading Committee 1986 -1987 Chair - Graduate Admissions Committee 1985 -1986 Chair - Seminar Committee 1987-1988 Ad hoc Committee for evaluation of promotion and tenure procedures 1988 - 1990 Chair - Courses and Curriculum Committee 1988 - 1989 Committee to evaluate the foreign language requirement for the PhD 1988 - 1989 Divisional Representative to the Graduate Student Admission Committee 1988 - present Faculty advisor for College of Veterinary Medicine Open House 1989 - 1992 Chair - Graduate Admissions Committee 1990 - 1992 Chair - Graduate Admissions Committee 1990 - present Nontraditional Veterinary Medicine Student Admissions Committee 1990 - present Member (Chair of 2) of 3 faculty promotion and tenure committees 1991 - 1993 Courses and Curriculum Committee 1992 - 1993 Chair, Courses and Curriculum Committee 1994 Representative to the 13th Annual Veterinary Medical Education Symp. 1994 Committee to Evaluate Promotion and Tenure Procedures 1995-1998 Teaching Evaluation Committee 1995-1996 Chair, Microbiology/Immunology Faculty Search Committee 1996 Capricious Grading Committee 1997 Chair, ad hoc Committee on Faculty Evaluation 1998 Retreat on Teaching - Section Chair 1998 Participant in Departmental co-sponsored Grant Writer’s Workshop 1999-2000 Chair, Division of Microbiology and Immunology 2000-2001 Chair, Graduate Advisory Committee 2001 – present Advisory Committee 2006- Chair, Faculty Search Committee 2006 Chair, Pathobiology/Entymology Faculty Search Committee 2008 Chair, Pathobiology Faculty Search Committee

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2010 – present Course Coordinator, VM607 (10 credit hour, Pathobiology II, IIVPC) COLLEGE OF VETERINARY MEDICINE 1986-1989 Library Committee 1986-1987 Student Affairs Committee 1987-1988 Chairman - Student Affairs Committee 1988 - 1989 Committee to establish the L. E. Boley Clinical Resident Research Award 1988 - 1990 Chair - Library Committee 1989 - 1991 Ad hoc member, Courses and Curriculum Committee 1991 - 1994 Courses and Curriculum Committee 1994 - 2000 Scholastic Petitions Committee 1994 - 1995 ad hoc Committee on Instructional Strategies 1994 Invited Speaker, Continuing Education and Public Service (CEPS), Col-

lege of Vet. Med., on Research Careers in Veterinary Medicine- 1994 Core Faculty - Toxicologic Pathology Training Program 1995 - 1998 Chair, ad hoc Committee on Instructional Strategies 1996 Chair, Scholastics Petition Committee 1996 Participant/Panel Member - Teaching Seminar Series 1996 Student Discipline Committee, Chair 1997 1996 Research Advisory Committee 1997 Dean’s Committee on Development of an Interdisciplinary PhD Program 1997-1998 Scholastics Petition Committee 1998 Chair Student Discipline Committee (served 2 months) 1998 CVM Admissions Committee (served 2 months) 1999-2003 Research Advisory Committee 2006-2008 Courses and Curriculum Committee, (Co-Chair) 2008 Deans High Performance Graduate Review Team 2008-2011 Courses, Curriculum, and Educational Policy Committee 2010-2013 Promotion and Tenure Committee UNIVERSITY 1989 - present Biotechnology/Cell Science Center Faculty Advisory Committee 1992 - 1993 Elected to University of Illinois Faculty Senate 1994 - 1996 Elected to University of Illinois Faculty senate 1992 - present Cell and Molecular Biology Training Grant Faculty 1993 - 1997 University of Illinois Biotechnology Center 1995 - 1997 Senate Committee on Student Discipline 1996 - 1997 Immunology Resource Center Advisory Committee 1997 - 1998 Chair, Senate Committee on Student Discipline 2001 – 2002 Institutional Animal Care and Use Committee 2001 - present Chair, Institutional Animal Care and Use Committee 2004 - 2008 Director, Institutional Animal Care and Use Program, Office Vice Chancel-

lor for Research (50% service appt) EXTRAMURAL- PEER REVIEWER ACTIVITIES 1985 - present Ad hoc reviewer for the journal Analytical Biochemistry 1988 - present Ad hoc grant reviewer for Research Board, University of Illinois 1988 - 1989 Ad hoc grant reviewer for National Science Foundation-Molecular Mecha-

nisms of Cell-Cell Recognition and Adhesion 1989 - 1990 Ad hoc reviewer for Journal of Parasitology 1991 - present External Grant Reviewer for USDA Competitive Grants

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1995 USDA Review Panel (Virology), National Research Initiative (NRI) Com-petitive Grants Program, Washington D.C.

1997 USDA Review Panel (Virology), National Research Initiative (NRI) Com-petitive Grants Program, Washington D. C.

1994 - 1996 Scientific Advisor for Trends in Glycoscience and Glycotechnology ( 1997 Ad hoc reviewer for Proceedings National Academiy of Science 1998 Ad hoc reviewer for Journal of Veterinary Research 1998 USDA Review Panel, Panel Manager (Virology), National Research Initi-

ative (NRI) Competitive Grants Program, Washington D.C 1998 Ad hoc reviewer for Journal of Veterinary Pathology 2000 USDA Review Panel, Panel Manager (Virology), National Research Initi-

ative (NRI) Competitive Grants Program, Washington D.C. 2001 – 2002 National Board of Veterinary Medical Examiners (NBVME) 2005 USDA Review Panel, Panel Manager (Animal Protection), National Re-

search Initiative (NRI) Competitive Grants Program, Washington D.C. 2007, 2008 USDA Review Panel Member (Water and Watersheds), National Re-

search Initiative (NRI) Competitive Grants Program, Washington D.C EDITORIAL BOARDS 1996 - present Trends in Glycoscience and Glycotechnology 1999 – 2002 Journal of Veterinary Pathology 2010 – present Nutrients COMMUNITY 1991 - 1999 Champaign Park District Youth Coach/Assistant Coach (Baseball, Soccer,

Baskeball) 1994 Guest Speaker - Elementary School (Champaign Unit 4 Magnet School)

- 3rd Grade Class-Science Unit 1994 Guest Speaker - Booker T. WashingtonSchool (Champaign Unit 4 Mag-

net School) - 5rd Grade Class Science Unit 1994 Guest Speaker - Franklin Middle School (Champaign Unit 4 Magnet

School) - 6rd Grade Class Science Unit on the HumanSkeleton: Compar-ison with various animal species - presented to 60 students (2 classes).

1994 Guest Speaker - Franklin Middle School (Champaign Unit 4 Magnet School) - 6rd Grade Class Science Unit on comparison of the Human and Animal Gastrointestinal Tract -Presented to 60 students (2 classes).

1994 Illinois Science Olympiad (Regional) - Event Supervisor 1995 Illinois Science Olympiad (State) - Event Supervisor 1995 Awarded Life Membership in Illinois PTA 1996 Illinois Science Olympiad (State) - Event Supervisor 1995 Judge, Franklin Middle School Invent-America competition 1996 Judge, Franklin Middle School Invent-America competition 1996 Guest Speaker - Franklin Middle School (Champaign Unit 4 Magnet

School) - 6rd Grade Class Science Unit on the Human Skeleton: Com-parison with various animal species - presented to ~250 students (7 clas-ses)

1998 Invited speaker - Villa Grove High School, Villa Grove, IL - Science Ca-reers

RESEARCH EXPERIENCE:

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University of Louisville (with Professor Robert H. Glew): Metabolic Studies: 1. The measurement of mitochondrial biogenesis and glycoprotein synthesis in the ger-

minating Castor Bean. a. Use of radioactive precursors and cytoplasmic and mitochondrial protein synthesis

inhibitors. b. Measurement of respiration using the Warburg Apparatus and oxygen electrodes. 2. Subcellular fractionation and purification of mitochondria. 3. Fractionation and purification of radiolabeled natural products (complex carbohydrates)

using conventional chromatographic, acrylamide gel, and high voltage paper electro-phoresis techniques.

University of Pittsburgh School of Medicine (with Professor Robert H. Glew): 1. Subcellular fractionation and marker enzyme analyses: a. Purification of various subcellular ogranelle. b. Mitochondrial subfractionation. 2. Protein/glycoprotein purification: The development of a large scale purification proce-

dure for human serum alpha-1-antitrypsin; this procedure resulted in the resolution of three isomeric forms of alpha-a-antitrypsin based on sialic acid microheterogeneity.

3. Physical-Chemical characterization of proteins/glycoproteins using the following meth-odology:

a. Ultracentrifugation analyses (sedimentation velocity and equilibrium procedures. b. Ion-exchange, gel filtration, affinity, adsorption, and paper chromatography.

c. Electrophoresis: acrylamide slab, disc gel, crossed antigen/antibody, immuno- and high voltage paper.

d. Purification of glycopeptides, amino acid analyses, carbohydrate compositional analyses, gas chromatography, and peptide mapping techniques.

4. Enzymology: a. Quantitation of human serum glycoprotein glycosyltransferase activities in normal

individuals and patients with alpha-1-antitrypsin deficiency and juvenile hepatic cir-rhosis.

b. Comparison of the kinetic properties of human serum and liver alpha-1-antitrypsin sialic acid transferases.

These studies resulted in the first demonstration of a generalized glycoprotein sialic acid transferase deficiency associated with liver cirrhosis due to alpha-1-antitrypsin deficiency in humans

The Johns Hopkins University (with Professor Saul Roseman):

Studies on the Specific Intercellular Adhesion of Hepatocytes

Cell Biology: 1. Isolation, purification, and characterization of chicken and rat liver hepatocytes: a. In vivo liver perfusion techniques. b. Light microscopic techniques including phase contrast, darkfield, Nomarski optics,

and epi-fluorescence. 2. Isolation and purification of Kupffer cells from rat and chicken liver. 3. Isolation (by in vivo perfusion) and purification of heart myocytes from rats and chick-

ens.

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4. Tissue culture techniques: a. Normal procedures for maintaining and transferring sterile fibroblast and epithelial

cell cultures. b. development of a procedure for confluent static monolayer culture of primary

hepatocytes from rats and chickens. 5. Development of in vitro biological assays for the quantitation and differentiation of spe-

cific and nonspecific intercellular adhesion among chicken and rat hepatocytes and heart myocytes.

6. Surgical procedures for partial hepatectomies in rats and chickens and isolation of hepatocytes from regenerating liver.

Biochemistry: 1. Metabolic studies: a. Measurement of the kinetic of synthesis of sulfated glycosaminoglycans and protein

during in vitro re-adhesion of chicken hepatocytes. b. Measurement of the kinetics of uptake of glycoproteins and neoglycoproteins by iso-

lated chicken and rat hepatocytes. 2. Development of a zonal centrifugation procedure for the large scale isolation and purification of plasma membranes from chicken liver. 3. Development of automated enzyme analyses for the use in measurement of cell num-

ber and quantitation of cell adhesion rates. 4. Isolation, purification, and characterization of cell-surface molecules

involved in specific and nonspecific adhesion of chicken hepatocytes. 5. Preparation and use of monospecific antibodies made against the chicken hepatocyte

carbohydrate binding protein. 6. Studies on the binding of chicken hepatocytes to monosaccharides immobilized on

polyacrylamide gels. 7. Lipid biochemistry - the quantitative isolation, purification, and characterization of

phospholipids and gangliosides from various tissues using the following methodology: a. Large scale solvent extraction and partitioning column chromatography (ion-

exchange, adsorption, HPLC and gel-filtration). b. Analytical and preparative thin-layer chromatography including HPTLC, reverse-

phase, and quantitative densitometry. c. Structural analyses of gangliosides. University of Illinois: 1. Development of procedures for the isolation, purification and culture of primary dog

hepatocytes. 2. Development of procedures for repeated in vivo liver biopsy in dogs and microscale

quantitiation of a various biomolecules and liver enzyme activities. 3. Development of rapid, computer-assisted morphometric techniques for kinetic quantita-

tion of hepatocellular swelling in dogs with glucocorticoid hepatopathy. 4. Production of monoclonal antibodies and development of ELISA (microtiter and dot)

assays for identification and quantitation of a variety of antigen (dog intestinal alkaline phosphatase, chicken serum mannose-binding protein, chicken liver cell-cell adhesive ganglioside).

5. Development of high-yield purification procedures for the isolation of infective sporozoites from Coccidian parasites.

6. Purification and characterization of acid phosphatase activity of Eimeria vermiformis. 7. Determination of the effects of glucocorticoids on hepatic asialoglycoprotein receptor-

mediated endocytosis.

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8. Determination of the information role of complex carbohydrates on ligand routing and sorting following receptor-mediated endocytosis.

9. Purification and characterization of bacterial acid phosphatases and evaluation of their role as virulence factors.

10. Identification and characterization of intestinal epithelial cell surface receptors involved in binding protozoan and viral pathogens.

11. Determination of carbohydrate composition and fine structure of gangliosides. 12. Development of methods for collection, storage and purification of large-scale amount

of Cryptosporidium parvum oocysts and sporozoites 13. Development of assays for measurement of microbial adhesion, invasion and growth of

Cryptosporidium parvum in human and animal intestinal cells 14. Development and use of intestinal xenografts as model systems for studying

enteropathogens. 15. Discovery of the porcine intestinal receptor for porcine Group A rotavirus 16. Synthesis of a receptor mimetic for treatment of porcine rotavirus disease 17. Development of methodology for quantitative measurement of overland transport, re-

covery, fate, and microbial adhesion of rotavirus and Cryptosporidium parvum oocysts to soil and vegetation in collaboration with the Department of Agricultural Engineering

18. Development of nanomechanical approachs to understanding metastasis through cell adhesion measurement

19. Discovery of the influence of mechanical force in regulation of cancer cell metastasis

RESEARCH INTERESTS: Microbial Adhesion: Initial events in host recognition and establishment of infectious disease Host-Parasite Interactions: As model systems for investigating the molecular mechanisms of cell recognition and adhesion, and complex carbohydrate structure and function. Cell Surface Carbohydrate Biochemistry: The structure, function, and metabolism of cell surface glycoconjugates: as they relate to cell-cell recognition, cell communication, and infectious dis-ease. Nutriceuticals: Development for the prevention and treatment of gastrointestinal infectious dis-ease Mechanosensing: Role of in cancer cell adhesion and metastasis CURRENT RESEARCH SUPPORT: NIH RO1 HD08-004 – Mechanism by which Human Milk Oligosaccharides Protect Against Rota-

virus (Sharon M. Donovan PI; Mark S. Kuhlenschmidt, CoPI) – 7-01-09 - 6-03-13 $1,505,750,00

NSF - NSF Science and Technology Center: Emergent Behaviors of Integrated Cellular Systems

(EBICS), Roger D.Kamm (PI), Mark S. Kuhlenschmidt (Senior Personnel, one of 24 scien-tists within three participating institutions (Massachusetts Institution of Technology University of Illinois at Urbana-Champaign, Georgia Institute of Technology), $25,000,000.00/5yrs, 9-1-10 – 8-30-15. (each senior personnel receives ~ $100,00.00/year direct costs.

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NIH/NCI - Training the next generation of researchers in cancer nanotechnology at the NCI M-CNTC (Midwest Cancer Nanotechnology Training Center) at the University of Illinois at Ur-bana-Champaign. Funded by the Alliance for Nanotechnology in Cancer. 10/1/2010-9/30/2015. PI: Rashid Bashir and Ann Nardulli, $388,800/year.

NSF – Regulation of cancer metastasis by mechanical force, Taher A. Saif (PI), Mark S.

Kuhlenschmidt (CoPI), NSF 1002165, $360,000.00.5-1-10 – 4-30-13, NIH – 1RO1AI094098-01A1 - Designing Selective Inhibitors of Calcium-Dependent Kinases in

Parasites, Mark S. Kuhlenschmidt (subcontract award ($218,136/3y) with Dr. David Si-bley, PI, Washington University School of Medicine (7/1/2012-6/30/15).

IN3 – Metastasis on a Dish; Taher Saif, Mark Kuhlenschmidt, Davendra Ramkumar, Tangella

Krishnarao. University of Illinois Interdisciplinary Innovation Initiative, $100,000.00 (7/1/12 – 6/30/13)

NIH RO1 HD08-004 Mechanism by which Human Milk Oligosaccharides Protect Against Ro-

tavirus 7/1/09-11/3/13 Mark S. Kuhlenschmidt, CoPI; Sharon M Donovan (PI) USDA-CSRS NC1041 Intestinal Disease of Pigs and Cattle; Mark S. Kuhlenschmidt (PI)

12/1/07-11/30/15 NIH – MCRE for Biodefense and Emerging Infectious Diseases; Targeting the double-stranded

RNA viral symbiont of Cryptosporidium parvum, Mark S. Kuhlenschmidt (University of Illi-nois Subaward), Steven Beverley, Washington University PI, 03/01/2013 - 07/28/2014

GRANT PROPOSALS SUBMITTED - PENDING REVIEW: None GRANT PROPOSALS in preparation: NIBIB T32: Imaging and Engineering Tissue Microenvironments NIH: Mechanism of Mechanical Microenvironmental Control of Cancer Metastasis PREVIOUS RESEARCH SUPPORT: 1. 1983 - BRSG: "Cell-Cell Recognition and Tumor Metastasis: The Role of Cell Surface

Gangliosides." PI: Mark S. Kuhlenschmidt; $3,000. 2. 1984 - BRSG: "Mechanism of Target-Cell Recognition in Eimeria vermiformis." PI: Mark

S. Kuhlenscmidt; $3,000. 3. 1985 - Research Board, University of Illinois: "Cushing's Syndrome: Mechanism of Produc-

tion of Corticosteroid-Induced Alkaline Phosphatase in Liver." PI: Mark S. Kuhlenschmidt; $5,500.

4. 1984 - Hatch Funds: "Antigenic Relatedness Among Three Stains of Anaplasma

marginale." PI: Ron D. Smith; CoPI: Mark S. Kuhlenschmidt; $32,000.

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5. 1985-1988 - Morris Animal Foundation Grant: "Cushing's Syndrome: Production of Alkaline Phosphatase and its Association with Hepatocellular Vacuolation." PI: Mark S. Kuhlenschmidt; Direct costs: $76,600.

6. 1987-1988 - Biomedical Research Support Grant: "Septicemic Erysipelas in Mice: A Model

System for Evaluation of Neuraminidase as a Virulence Factor." PI: Mark S. Kuhlenschmidt; $3,000.

7. 1988-1992 - U.S. Army Medical Research and Development Command - "Pathophysiology

and Toxicokinetic Studies of Blue Green Algae Intoxication in the Swine Model." PI: Val Beasley; Direct costs: $1,198,773.00; coinvestigators: Dr. Wanda Haschek-Hock and Mark S. Kuhlenschmidt.

8. Illinois Agriculture Experiment Station - Prevention and Control of Enteric Diseases of

Swine." PI: Gail Scherba; CoPI: Mark S. Kuhlenschmidt; $70,000; 10/1/87-9/30/92. 9. 1991-1992 - Illinois Department of Agriculture - Evaluation of the Therapeutic Potential of an

Enterocyte Receptor for Porcine Rotavirus. PI: Mark S. Kuhlenschmidt; Co-PI: Howard Gelberg; Direct Costs: $33,500.

10. 1989-1993 - NIH (National Eye Institute) - "Use of Assays to Investigate Acanthamoeba -

Cornea Interactions." PI: Gerald McLaughlin; CoPI: Mark S. Kuhlenschmidt; Direct costs: $368,728/4 years.

11. 1990-1993 - USDA - "Identification, Isolation, and Characterization of the Porcine Enterocyte

Receptor for Group A Rotavirus." Co-PI: Mark S. Kuhlenschmidt; Howard B. Gelberg; Direct costs: $149,878.

12. 1993 - 1994 - Biomedical Research Development Corporation (BDRC): Efficacy of

Sialyloligosaccharides For Control of Rotavirus Disease in Swine. Direct Costs: $60,000 - PI: Mark S. Kuhlenschmidt, CoPI: Howard Gelberg. .

13. 1993 - 1994 - U of I Research Board: Cloning of the Acid Phosphatase Gene of Francisella

tularensis, PI: Mark S. Kuhlenschmidt, $16,635.00, 14. 1992-1995 - Illinois Department of Agriculture (IDA) - “Characterization of the Porcine Enter-

ocyte Receptor for Group A Rotavirus”, Co-PI: Mark S. Kuhlenschmidt, Howard B. Gelberg; Direct costs: $60,000.

15. USDA-NRI - An Enterocyte For Porcine rotavirus: Sturcture and Therapeutic Efficacy. Direct

Costs: $181,000 (6/1/93 - 5/31/96). PI: Mark S. Kuhlenschmidt, CoPI: Howard Gelberg 15. IDA (Illinois Department of Agriculture): Preliminary Isolation and Characterization of a Bo-

vine Enterocyte Receptor for Cryptosporidium parvum Infection: PI: Mark S. Kuhlenschmidt $40,000 1/94 - 12/95.

16. USDA -NRI (Renewal) - “Receptor Therapy for Porcine Group A Rotavirus Disease”, PI:

Mark S. Kuhlenschmidt, Co PI: Howard Gelberg $294,000.00 (9/30/96 - 9/29/99) - ranked #1 proposal in Virology Panel Study Section)

17. USDA -NRI (Renewal) - “Receptor Therapy for Porcine Group A Rotavirus Disease”, PI:

Mark S. Kuhlenschmidt, Co PI: Howard Gelberg $298,000.00 (9/30/99 - 9/29/02) - ranked #3 proposal in Virology Panel Study Section)

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18. IDA (Illinois Department of Agriculture) - Contribution of Dairy Cattle Fecal Waste to the En-vironmental Prevalence of Cryptosporidium parvum PI: MS Kuhlenschmidt, CoPI: RD Smith,., 1998-1999, $8000

19. IDA (Illinois Department of Agriculture) - Survival and Viability of Cryptosporidium parvum

under simulated Farm Waste Management Conditions, PI: RD Smith, CoPI: MS Kuhlenschmidt, 1999-2000, $8900.

20. NIH PHS 1 R21AI44967 - Anti-Adhesive Receptor Mimetics for Cryptosporidiosis $241,000

PI: Mark S. Kuhlenschmidt, (1999 – 2002). 21. C-FAR (Illinois Council on Food and Agriculture Research) - An Integrated Approach to Re-

duce Pathogen and Nutrients in Runoff from Animal Production Systems, PI: Prasanta Kalita, CoPI: Mark S. Kuhlensdhmidt, $149,750, 2001-2003,

22. USDA -NRI (Competitive Renewal) - “Receptor Therapy for Porcine Group A Rotavirus Dis-

ease”, PI: Mark S. Kuhlenschmidt, $256,000.00 (9/30/02 - 9/29/05. 23. USDA – Hatch - Differentially Expressed Early Invasion Genes of Cryptosporidium parvum PI: Mark S. Kuhlenschmidt. 60,000/3 yr. (2002-2005) 24. USDA – MGET/IFAFS– 2001-52100-11527- Agricultural Genome Sciences and Public Poli-

cy Training Program. CoPI: Lawrence B. Schook, Harris Lewin, Lila Vodkin;,CoI: Mark S. Kuhlenschmidt. $2,200,000, 10/1/01-9/30/05

25. USDA – MGET/IFAFS – Graduate Student Research Fellowship for Joann Schmidt (2 years

support (covers tuition, fees and stipend) 26. USDA – Animal Health - Efficacy of Porcine Milk Oligosaccharides for the Prevention and

Treatment of Rotavirus Disease, Mark S. Kuhlenschmidt, PhD (PI), Sharon M. Donovan, PhD (Nutritional Sciences) (CoPI) and Karen Ochonicky (graduate student), $60,000/3yrs, includes research assistantship for Karen Ochonicky (DVM, MS student).

27. Illinois Department of Agriculture (IDA) - Clinical and Clinicopathological Effects of

Cryptospordial Infection in Dairy Calves, Peter D. Constable (PI), Mark S. Kuhlenschmidt (CoPI), $19,000/1 year

28. USDA-CSRS Regional Research Funds – NC 1007 - Enteric Disease of Swine. PI: Mark S.

Kuhlenschmidt. 14,000/yr. (2000-2005). 29. Bristol-Myers-Squibb, Freedom to Discover Grant, PI: Sharon Donovan, CoI: Mark S. Kuhlenschmidt, "Defining the Nutritional Requirements of the Developing Intestinal Eco-

system", $500,000.00/5yrs 1/2005 – 12/2010.

30. USDA-NRI - Control Cryptosporidium and rotavirus contamination of agricultural water-sheds, Mark S. Kuhlenschmidt (PI), Prasanta Kalita (CoPI), $400,000/3yrs, 8/06-7/10.

31. USDA-Animal Health & Disease - Mechanisim of Cryptosporidium parvum Sporozoite-Host

Cell Invasion, Mark S. Kuhlenschmidt (PI), $20,000.00 10/1/2006-9/30/2007. 32. CFAR - A Novel Method for Disease Control in Livestock: DDGS, James Pettigrew (PI),

Mark S. Kuhlenschmidt, (CoI), $285,000.00, 12/06 – 11/09.

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33. NSF – A nanomechanical approach to understanding metastasis through cell adhesion measurement, Taher A Saif (PI), Mark S. Kuhlenschmidt (CoPI), NSF 0725831, $392,863, 9-1-07 – 7-31-11.

34. NIH – Mechanisms of Pseudomonas Resistance to SP-A Mediated Membrane

Permeabilization; G. Lau (PI), Mark S. Kuhlenschmidt, (CoPI), $1,891,894.00, 12/1/07-11/30/12

35. Abbott Nutrition, Inc – Effect of Human Milk Oligosaccharides on Infectivity of Selected Res-

piratory Virus Pathogens: Mark S. Kuhlenschmidt (PI), $275,000.00, 10-1-09 - 12-30-10. INVITED PRESENTATIONS (University of Illinois) 1. June 27, 1983 - Cell Contact and Adhesion Gordon Conferences: Isolation and Partial

Characterization of a Ganglioside Fraction that Participates in Cell-Cell Recognition Among Chicken Hepatocytes.

2. November 29, 1983 - American Society for Cell Biology 23rd annual meeting, Minisymposia

#7: The Involvement of A Ganglioside Fraction in Chicken Hepatocyte Cell-Cell Recogni-tion.

3. May 1985 - Minisymposium on Tropical Disease Research, Allerton Park, University of Illi-

nois: Mechanism of Cell-Cell Recognition among Chicken Hepatoctyes. 4. March 17-20, 1985 - Invited Consultant, National Institute of Neurological and Communica-

tive Disorder and Stroke: The Amyotrophic Lateral Sclerosis Workshop on Amyotrophic Lateral Sclerosis.

5. May 12, 1989 - Invited Speaker, GlycoMed, Inc., Alameda, CA: Cushing's Syndrome: A

Model System for the Reutilization of Glycoproteins by Receptor-Mediated Endocytosis. 6. May 18, 1989 - Invited Speaker, Department of Biology, The Johns Hopkins University:

Cushing's Syndrome: A Model System for the Reutilization of Glycoproteins by Receptor-Mediated Endocytosis.

7. October 3, 1991 - Invited Speaker, University of Illinois College of Veterinary Medicine Fall

Conference: Preliminary Identification of a Receptor for Porcine Rotavirus.

18. 1994 - Invited Speaker - “Friends of Francisella” meeting (organizer Dr. Francis Nano, Universtiy of Victoria, Victoria B. C.), Identification and Purification of a major acid phospha-tase of Francisella tularensis: Role as a putative virulence factor. May 24, Las Vegas, Ne-vada.

19. 1994 - Invited Speaker - Biotechnology Research and Development Corporation Annual

Workshop; Efficacy of Sialyloligosaccharides for control of rotavirus disease in swine. Au-gust 22-23, Ames, Iowa.

20. 1995 - Gordon Research Conference on Structure and Function of Glycolipids and

Sphingolipids, January 15-20, Ventura, CA.

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21. 1995 - Invited Speaker - First international Mt. Rushmore conference on Mechanisms in the Pathogenesis of Enteric Diseases, “Characterization of the porcine enterocyte receptor for Group A Rotavirus”, September 27-30, Rapid City, South Dakota

22. 1995 - Invited Session Chairperson - First Internation Mt. Rushmore conference on Mecha-

nisms in the Pathogenesis of Enteric Diseases, “Mechanisms of Identity and Interaction Be-tween Host and Pathogen.

23. 1997 - Invited Speaker - American Society of Biochemistry and Molecular Biology Satelite

Symposium., “Spingolipids as Mediators of Molecular Events.”

24. 1998 - Invited Session Chairperson., Second International Rushmore Conference on Mech-anisms in the Pathogenesis of Enteric Diseases, Rapid City, South Dakota. “Mechanisms of Identity and Interaction Between Host and Pathogen.”

25. 1999 - Second Annual International Conference on New and Re-Emerging Infectious Dis-

eases. University of Illinois, College of Veterinary Medicine, “Partial Purification of a Cryp-tosporidium parvum Sporozoite Host Cell Receptor from Plasma Membrane Vesicles.”

26. 2000 - Veterinary Pathobiology Seminar Series, Department of Pathobiology, College of

Veterinary Medicine, University of Illinois,“Receptor Therapy for Porcine Group A Rotavirus Disease.”

27. 2000 - Proceedings, 9th Symposium of the International Society for Veterinary Epidemiology

and Economics, Aug 6-11, 2000; Breckenridge, CO., “Critical control points for reducing en-vironmental contamination with Cryptosporidium parvum oocysts from dairy cattle.”

28. 2000 - Proceedings, 81st Annual Meeting of the Conference of Research Workers on Ani-

mal Diseases (CRWAD). Nov 12-14, 2000; Chicago, IL.,“Simulation model to predict envi-ronmental contamination with Cryptosporidium parvum oocysts from a dairy herd.”

29. 2001 - American Society of Agricultural Engineers Annual Meeting, Sacramento, CA Over-

land and Near-surface Transport of Cryptosporidium parvum”. J. R. Trask, P. K. Kalita, M. S. Kuhlenschmidt, R. D. Smith, and T. L. Funk.

30. 2001 - American Society for Microbiology Annual Meeting, May 20-24, 2001, Orlando, FL

“Inhibition of Porcine (OSU) Rotavirus Binding and Infectivity by a Sialyllactose Neoglycolipid”, D. W. Bergner, T. B. Kuhlenschmidt, M. S. Kuhlenschmidt

31. 2003 – Department of Pathobiology Seminar Series, College of Veterinary Medicine, Octo-

ber 8, 2003, Complex Carbohydrate Analyses by HPAEC-PAD (High pH Anion Exchange Chromatography-Pulsed Amperometric Detection

32. 2003 – Division of Nutritional Sciences, College of ACES, University of Illinois, Nov 19,

2003, Receptor Therapy for Porcine Rotavirus Disease

33. 2005 – BMRC Infectious Disease Symposium, Microbial Adhesion of Cryptosporidium parvum Sporozoites, January 19, 2005, Singapore

34. 2005 – National University of Singapore, Department of Microbiology, January 20, 2005,

Singapore

35. 2005 – National Dairy Council, University of Illinois, Dietary Intervention for Prophylaxis of Cryptosporidiosis, October 25, 2005.

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36. 2005 - University of Illinois, Department of Agricultural Engineering, Microbial Adhesiion of

Cryptosporidium parvum Sporozoites. Nov. 18, 2005.

37. 2005 – Kenote Speaker , Congress of Research Workers on Animal Diseases (CRWAD), Gastroenteric Diseases Division, Host Cell invasion by Cryptosporidium parvum sporozoites: A potential dietary intervention strategy. Dec 5, 2005, St. Louis, MO.

38. 2006 – Oregon State University College of Veterinary Medicine, Department of Biomedical

Sciences, Microbial Adhesiion of Cryptosporidium parvum Sporozoites, April 3-6, 2006. 39. 2007 – Invited speaker, USDA National Water Conference, Savannah, GA. 40. 2008 – Invited speaker, USDA National Water Conference, Reno, NV. 41. 2009 – Invited speaker, USDA National Water Conference, St. Louis, MO. ABSTRACTS (from the University of Illinois): 1. 1985 - 61st Annual Meeting of the American Society of Parasitologists, Denver, CO: Im-

proved Purification of Infective Sporozoites of Eimeria species. M. S. Kuhlenschmidt, J. E. Hosek, and K. S. Todd.

2. 1985 - Congress of Research Workers in Animal Diseases, Chicago, IL: Purification and

Biochemical Comparison of the Steroid-Induced and Intestinal Alkaline Phosphatase Isoenzymes. R. Sanecki, W. E. Hoffmann, M. S. Kuhlenschmidt, and J. L. Dorner.

3. 1986 - Annual Meeting of the American Association of Clinical Chemists, Chicago, IL: Puri-

fication and Biochemical Comparison of Corticosteroid- Induced and Intestinal Alkaline Phosphatase Isoenzymes. R. Sanecki, W. E. Hoffmann, M. S. Kuhlenschmidt, and J. L. Dorner.

4. 1986 - Federation of American Societies for Experimental Biology (American Society of Bio-

logical Chemists) annual meeting in Washington D.C.: Isolation and Characterization of Hepatocytes from Dogs with Canine Cushing's Syndrome. M. S. Kuhlenschmidt, W. E. Hoffmann, R. Sanecki, and M. K. Rippy.

5. 1986 - Annual Meeting of the American College of Veterinary Pathologists, New Orleans,

LA: Kinetics of Biochemical and Histological Changes Associated with Glucocorticoid-Induced Hepatopathy in the Dog. M. K. Rippy, W. E. Hoffmann, and M. S. Kuhlenschmidt.

6. 1987 - Annual Meeting of the American College of Veterinary Pathologists, Monterey, CA:

Alkaline Phosphatase Isozymes in the Dog are Covalently Attached to Membranes by a Specific Phospatidylinositol Anchor. M. K. Rippy, W. E. Hoffmann, and M. S. Kuhlenschmidt.

7. 1989 - 70th Annual Meeting of the Congress of Research Workers in Animal Disease, No-

vember 1989, Chicago, IL: Effects of Glucocorticoids on the Intestinal Alkaline Phospha-tase Isozyme in the Dog. M. S. Kuhlenschmidt, M. K. Rippy, and W. E. Hoffmann. Ab-stract number 219, p. 40.

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8. 1988 - Proceedings of the International Society of Toxicology (August 1988), Microcystin-A Induces Morphologic and Cytoskeletal Hepatocyte Changes In Vitro. S. B. Hooser, L. L. Waite, V. R. Beasley, W. W. Carmichael, M. S. Kuhlenschmidt, and W. M. Haschek.

9. 1989 - Proceedings of the Society of Toxicology - Microcystin-LR Induces Morphologic and

Cytoskeletal Changes In Vitro and In Vivo. S. B. Hooser, V. R. Beasley, M. S. Kuhlenschmidt, and W. M. Haschek.

10. 1990 - Proceedings of the American College of Veterinary Pathologists, 43rd annual meet-

ing, p. 205 - December 11-15, “Development, Characterization and Utilization of an Intesti-nal Xenograft Model for Infectious Disease Research”, Thulin, J. D., Gelberg, H. B. and Kuhlenschmidt, M. S.

11. 1990 - Hoffmann W. E. , Kuhlenschmidt, M. S., Solter, P. F., Rippy, M. K., Alpers, D. L.

Proposed mechanisms of the release of the corticosteroid-induced alkaline phaophatase isoenzyme into blood of dogs. IV. Congress of the International Society for Animal clinical Biochemistry.

12. 1991 - Proceedings of the American College of Veterinary Pathologists, 44th annual meet-ing, p. 182, December 12th, “Preliminar Characterization of a cellular receptor for Group A Porcine Rotavirus, Rolsma, M. D., Gelberg, H. B. and Kuhlenschmidt, M. S.

13. 1992 - Annual Meeting of the American Society of Microbiology, New Orleans “Identification

of a Ganglioside Fraction Involved in Porcine Rotavirus Recognition of MA-104 Cells and Enterocytes” Rolsma, M. D., Gelberg, H. B., and Kuhlenschmidt, M. S.

14. 1992 - Annual Meeting of the American Society of Microbiology, New Orleans, Purification

and Partial Characterization of an Acid Phosphatase from Fransciella tularensis that Inhib-its Superoxide Production by Neutrophils. Reilly, T., Williams, J., and Kuhlenschmidt, M.S.

15. 1992 - Proceedings of the American College of Veterinary Pathologists, 43rd annual meet-

ing, San Diego. Identification of a cellular receptor for group A porcine rotavirus. Rolsma, M. D., Gelberg, H. B. and Kuhlenschmidt, M. S. Vet. Pathol. 29:460.

16. 1992 - Proceedings of the American College of Veterinary Pathologists, 43rd annual meet-

ing, San Diego. An intestinal xenograft model for Cryptosporidium parvum infection. Thulin, J. D., Kuhlenschmidt, M. S., Rolsma, M. D., Muelbroek, J., Current, W. L., and Gelberg, H. B. Vet. Pathol. 29:461.

17. 1993 - Proceedings of the American College of Veterinary Pathologists, 44th annual meet-

ing, San Antonio, Role of enterocyte gangliosides in the pathogenesis of gastrointestinal disease. Rolsma, M. D., Gelberg, H. B. and Kuhlenschmidt, M. S. Vet. Pathol. 30:471

18. 1994 - American Society of Biological Chemists annual meeting in Washington D.C.: A

novel, respiratory burst-inhibiting acid phosphatase is a major surface antigen of Francisella tularensis. T. Reilly and M. Kuhlenschmidt.

19. 1994 - Proceedings: American College of Veterinary Pathologists, 45th Annual Meeting,

Montreal. Biochemical and temporal characterization of porcine enterocyte gangliosides that mediate binding of group A rotavirus. Vet Pathol 31:611 Rolsma, M. D., Kuhlenschmidt, M.S. and Gelberg, H.B.

20. 1995 - Gordon Research Conference on Structure and Function of Glycolipids and

Sphingolipids, January 15-20, Ventura, CA., Two closely related monosialogangliosides

21

with potent blocking activity for Group A Porcine Rotaviruses on enterocytes, M. S. Kuhlenschmidt, M.D. Rolsma, and H.B. Gelberg.

21. 1995 - Proceedings: American College of Veterinary Pathologists, 46th Annual Meeting,

Atlanta. Vet Pathol 32:589. Porcine enterocyte gangliosides: temporal changes in concen-tration and inhibition of group A rotavirus infectivity. Rolsma MD, Kuhlenschmidt MS, Gelberg HB.

22. 1996 - Proceedings: American College of Veterinary Pathologists, 47th Annual Meeting,

Seattle, WA. An in vitro cell-suspension assay to quantify binding of Cryptosporidium parvum sporozoites to host cells. Vet. Pathol. 33:612, J. K. Johnson, M. S. Kuhlenschmidt, and H. B. Gelberg

23. 1997 - Ringenberg M, Vimr E, Kuhlenschmidt T, Kuhlenschmidt M, Gelberg H. Structural

and quantitative aspects of glomerular sialylation utilizing an ex vivo sialidase release mod-el. Proceedings: American College of Veterinary Pathologists, 48th Annual Meeting, Albu-querque, Vet Pathol 34:, 1997.

24. 1997 - Johnson JK, Kuhlenschmidt MS, Gelberg HB. Glycoconjugates and plasma mem-

brane vesicles inhibit Cryptosporidium parvum sporozoite-host cell recognition. Proceed-ings: American College of Veterinary Pathologists, 48th Annual Meeting, Albuquerque, Vet Pathol 34: 1997.

25. 1997 - Ringenberg M, Gelberg H, Kuhlenschmidt T, Kuhlenschmidt M, Vimr E.Structure

and function of N-acylneuraminosyl residues in sialidase (neuraminidase)-induced renal failure XIVth International Symposium on Glycoconjugates, Zurich, Switzerland

26. 1998 - Gumprecht, L, Wollenberg, GK, Kuhlenschmidt, MS, Constable, P, Parker, HM,

Tumbleson, ME, Simutis, CA and Haschek, WM. The myotoxin fumonisin B1 alters sphingolipid biodynthesis and inhibits bacterial phagocytosis. Conference on New and Re-emerging Infectious Diseases, University of Illinois, Urbana, IL.

27. 1998 - Johnson, JK, Gelberg, H and Kuhlenschmidt, M. Glycoconjugates and plasma

membrane vesicles inhibit Cryptosporidium parvum sporozoite-host cell recognition Con-ference on new and Re-emerging Infectious Diseases, University of Illinois, Urbana, IL.

28. 1999 - Johnson, HB Gelberg, and MS Kuhlenschmidt - Partial Purification of a Cryptospor-

idium parvum Sporozoite Host Cell Receptor from Plasma Membrane Vesicles Second An-nual Conference on New and Re-Emerging Infectious Diseases, Urbana, Illinois.

29. 1999 - Gumprecht, GK Wollenberg, MS Kuhlenschmidt, PD,Constable HM Parker,,

ME,Tumbleson, CA Simutis and WM Haschek,- The Mycotoxin Fumonisin B1 Alters Sphingolipid Biosynthesis and Inhibits Bacterial Phagocytosis, Toxicological Sciences 48:54.

39. 1999 - M. Ringenberg, MS Kuhlenschmidt, T Kuhlenschmidt, G Koritz, E Vimr and H

Gelberg - Absorption and Disposition of Recombinant 125Iodine Labeled Vibrio Cholerae and Salmonella typhimurium strain LT2 Neuraminidase in the Rat, Veterinary Pathology 35:420.

40. 1999 - JK Johnson, HB Gelberg, and MS Kuhlenschmidt - Partial Purification of a Cryptos-poridium parvum Sporozoite Host Cell Receptor from Plasma Membrane Vesicles Second Annual Conference on New and Re-Emerging Infectious Diseases, Urbana, Illinois.

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41. 1999 - J. Johnson-Ward, RD Smith, and MS Kuhlenschmidt, Evaluation of the Premier Cryptosporidium by Meridian Diagnostics for the detection of C. parvum in dairy cattle. Con-ference for Research Workers in Animal Diseases Proceedings

42. 1999 - Johnson-Ward J, Smith RD, and Kuhlenschmidt, MS., Critical control points for re-ducing environmental contamination with Cryptosporidium parvum oocysts from dairy cattle, Procedings, 9th Symposium of the International Society for Veterinary Epidemiology and Economics, Aug 6-11, 2000; Breckenridge, CO.

43. 2000 - Johnson-Ward J, Smith RD, and Kuhlenschmidt, MS., Simulation model to predict environmental contamination with Cryptosporidium parvum oocysts from a dairy herd”, Pro-ceedings, 81st Annual Meeting of the Conference of Research Workers on Animal Diseases (CRWAD). Nov 12-14, 2000; Chicago, IL.

44. 2001 - J. Trask, S. McLaughlin, MS Kuhlenschmidt and P. Kalita 2003 Overland and Near-surface Transport of Cryptosporidium parvum, American Society of Agricultural Engi-neers, Sacramento, CA – July 30-Aug. 1, 2001

45. 2002 - Salto, M.L,, Kuhlenschmidt, T., Kuhlenschmidt, M., de Lederkremer, R.M. and Docampo, R. (Presence of glycoconjugates in acidocalcisomes from Trypanosoma cruzi. 5th Annual Conference on New and Re-Emerging Infectious Diseases. Urbana, April 18-19, p. 22.

46. 2002 - Salto, M.L., Kuhlenschmidt, T., Kuhlenschmidt, M., de Lederkremer, R.M. and Docampo, R. Presence of glycoconjugates in acidocalcisomes from Trypanosoma cruzi. XIII Molecular Parasisitology Meeting, Wood Hole, MA, September 22-26, p. 234A.

47. 2003 – SJ McLaughlin, PK Kalita, RA Cooke, SP Fox and MS Kuhlenschmidt. Adsorption Kinetics of Cryptosporidium parvumto Soils. American Society of Agricultural Engineers, Las Vegas, Nevada – July 27-30, 2003.

48. 2004 - Salto, M.L., Kuhlenschmidt, T., Kuhlenschmidt, M., de Lederkremer, R. M., and Docampo, R. Lipid composition of acidocalcisomes of Trypanosoma cruzi. 7th Annual Con-ference on New and Re-Emerging Infectious Diseases. Urbana, April 15-16, p. 23. http://www.cvm.uiuc.edu/idc/

49. 2005 - Ochonicky K, Donovan, S, Kuhlenschmidt T. and Kuhlenschmidt M. Inhibitory ef-fects of human and porcine milk oligosaccharides on sialic acid dependent and sialic acid independent strains of rotavirus. American Dairy Science Association Annual Meeting, Cincinatti, OH, July 24-28, 2005.

50. 2005 - Ochonicky K, Donovan S. Kuhlenschmidt T, Jimenez-Flores R, Kuhlenschmidt M. Inhibitory activity of bovine milk fat globule membrane against sialic acid-dependent and –independent strains of rotavirus American Dairy Science Association Annual Meeting, Cincinatti, OH, July 24-28, 2005.

51. 2005 - Andres A., Donovan S.M., Kuhlenschmidt T.B., Kuhlenschmidt M.S. Isoflavones at concentrations present in soy-based infant formula inhibit rotavirus infectivity in vitro. FASEB Jorunal 2005; 19:A446

52. 2005 – Andres A, Donovan, SM, Kuhlenschmidt TB, Kuhlenschmidt, MS. Genistin at con-centrations present in soy-based infant formula inhibits rotavirus infectivity in vitro through inhibition of protein tyrosine kinase activity. 6th International Symposium on the role of Soy in Preventing and Treating Chronic Disease, Oct. 30-Nov. 2, 2005, Chicago, IL.

53. 2007 - Gastrointestinal Aadaptive and Innate Immunological Responses to Rotavirus Infec-tion in the Neonatal Piglet. A. Andres, S.A. Taylor, B.A. Helregel, T.B. Kuhlenschmidt, M.S. Kuhlenschmidt, and S.M. Donovan; Digestive Disease Week, Los Angeles, CA; May 20-25

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54. 2007 - Gastrointestinal Structural and Functional Responses to Rotavirus Infection in the Neonatal Piglet. R.A. Mathai, A. Andres, B.A. Helregel, T.B. Kuhlenschmidt, M.S. Kuhlenschmidt, and S.M. Donovan; Digestive Disease Week, Los Angeles, CA; May 20-25

55. 2008 - Leonardo A. Gutierrez, Xuan Li, Jinwen Wang, Gordon N. Nangmeny, James Economyj, Theresa B. Kuhlenschmidt, Mark S. Kuhlenschmidt, and Thanh H. Nguyen; Study of sorption and inactivation of ms2 phage and rotavirus using iron oxide-coated glass fiber. ACS National Meeting, New Orleans, LA, April 6-10, 2008.

56. 2009 - Rose Ann Mathai, Kelly A. Tappenden, Theresa B. Kuhlenschmidt, Mark S. Kuhlenschmidt, SharonM. Donovan; Gastrointestinal structural and functional responses of piglets to soy based infant formulas with varying isoflavone content in compared to cow milk based formula. Annual Meeting of the Society for Pediatric Research. FASEB J. 22:896

57. 2009 - Yuanyuan Liu, Mark S. Kuhlenschmidt, Theresa B Kuhlenschmidt, Peter M. Yau, Thanh H. Nguyen; Role of Oocyst Wall Protein on Deposition Kinetics of Oocysts on Natural Organic Matter Surface: A Proteomics and Deposition Study. WaterCAMPWS Meeting, Uni-versity of Illinois, April 2009.

58. 2009 – Jennifer Lee, Milton McAllister, Theresa Kuhlenschmidt, and Mark S. Kuhlenschmidt; Long-chain polyunsaturated fatty acids inhibit infectivity of Apicomplexan parasites, Experimental Biology, April 2009

59. 2009 - V. Perez, C. Jacobs, C. Parsons, J. Barnes, M. Kuhlenschmidt, M. Jenkins, J. Pet-tigrew; Changes in gut microbiota of broiler chicks fed distillers dried grains with solubles (DDGS) during a coccidial infection, National Meeting of the American Society of Animal Science

60. 2009 - Paul Davidson, Daniel Koch, Theresa B. Kuhlenschmidt, Prasanta Kalita, and Mark S. Kuhlenschmidt; Interaction of Rotavirus and Cryptosporidium parvum Oocysts with Soil Particles and Vegetative Filter Strips, National Water Conference, St. Louis, MO.

61. 2009 - Measuring the surface adhesion of cancer cells: A novel way to characterize and

understand cancer metastasis. Xin Tang, Tony Cappa, Theresa B. Kuhlenschmidt, Mark S. Kuhlenschmidt, Taher A. Saif (Society of Engineering Science, Oct. 12-15.

62. 2011 - Min Li, Laura L. Bauer, Xin Chen, Theresa Kuhlenschmidt, Mark S. Kuhlenschmidt, George C. Fahey Jr., Sharon M. Donovan. In vitro fermentation of Human milk oligosaccha-rides by ascending colon microbiota from formula-fed and sow-reared piglets. FASEB J March 17, 2011 25:985.3

63. 2011 - Xin Chen, Min Li, Theresa B. Kuhlenschmidt, Mark S. Kuhlenschmidt, and Sharon M. Donovan. Effect of human milk oligosaccharides on rotavirus infectivity in MA104 cells. FASEB J March 17, 2011 25:584.1

64. 2011 - Yao Li, Theresa B. Kuhlenschmidt, Mark S. Kuhlenschmidt, and Sharon M. Donovan. Isoflavones reduce rotavirus infectivity in MA104 cells through inhibition of protein kinases in the JNK and p70 signaling pathways. FASEB J March 17, 2011 25:584.18

65. 2011 – Xin Tang, Theresa B. Kuhlenschmidt, Jiaxi Zhou, Philip Bell, Fei Wang, Mark S.

Kuhlenschmidt and Taher A. Saif. Discovery of an Intracellular-force-regulated Metastasis-like Phenotype in HCT-8 Cells. Gordon Research Conferences, June 19-24.

66. 2011 - Xin Tang, Theresa B. Kuhlenschmidt, Jiaxi Zhou, Philip Bell, Fei Wang, Mark S.

Kuhlenschmidt, Taher A.Saif. Mechanical force affects expression of an in vitro metastasis-like phenotype in HCT8 cells. Biophysical Society Annual Meeting, Baltimore, MD March 5-9.

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67. 2011 – Xin Tang, Theresa B. Kuhlenschmidt, Mark S. Kuhlenschmidt, and Taher A.Saif.

Cancer cell force evolves throughout in vitro cancer metastasis. Biomedical Engineering Society Annual Meeting, October 12-15, Hartford, CT.

68. 2012 - Shelly N. Hester, Sarah S. Comstock, Marcia H. Monaco, Theresa B. Kuhlenschmidt,

Mark S. Kuhlenschmidt, and Sharon M. Donovan. Acidic human milk oligosaccharides inhibit acute rotavirus infection with no effect of nucleotides in neonatal piglets, Experimental Biol-ogy Annual Meeting.

69. 2013 – Li Min, Monaco Marcia H, Wang Mei, Comstock Sarah S. Kuhlenschmidt Theresa B,

Kuhlenschmidt Mark S, Donovan Sharon M. Human milk oligosaccharides and prebiotics reduce the duration of rotavirus-induced diarrhea in piglets, European Society for Paediatric Gastroenterology, Hepatology, and Nutrition (ESPGHAN), May 8-11, London, England.

70. 2013 – Tang X, Saif TM, Kuhlenschmidt TB, and Kuhlenschmidt MS. Genetic Pathway

Analysis for Mechanics-induced Colon Cancer Metastasis, Biomedical Engineering Society (BMES) Annual Meeting, September 25-28, 2013 in Seattle, Washington

PUBLICATIONS: 1. Glew, RH, Kayman, SC, and Kuhlenschmidt, MS (1973) Studies of the Binding of

Concanavalin A to Rat Liver Mitochondria. J. Biol. Chem. 248:3137. 2. Kuhlenschmidt, MS, Yunis, EJ, Iammarino, RJ, Turco, SA, Peters, SP, and Glew, RH

(1974) Demonstration of Sialyltransferase Deficiency in the Serum of a Patient with Alpha-1-Antitrypsin Deficiency and Hepatic Cirrhosis. Lab. Invest. 31:413.

3. Kuhlenschmidt MS, Coffee CJ, Peters SP, and Glew, RH (1975) Serum Sialyltransferase

Activity in Alpha-a-Antitrypsin Deficiency and Hepatic Cirrhosis. In: Proteases and Biologi-cal Control, Cold Spring Harbor Laboratory, eds., E. Reich, D. B. Rifkin and E. Shaw, p. 415.

4. Miller RR, Kuhlenschmidt MS, Coffee CJ, Kuo I, and Glew RH. (1976) Comparison of the

Chemical, Physical and Survival Properties of Normal and Z-Variant Alpha-1-Antitrypsins. J. Biol. Chem. 251:4751.

5. Miller RR, Peters SP, Kuhlenschmidt MS, and Glew RH (1976) The Use of Ion-exchange

Resins in the Application of Protein Samples to Gel Filtration Columns. Anal. Biochem. 72:45.

6. Kuhlenschmidt MS, Peters SP, Pinkard OD, Glew RH, and Sharp H. (1977) Cytidine-5'-

monophosphate N-Acetylneuraminic Acid Asialoglycoprotein Sialic Acid Transferase Activity in Liver and Serum of Patients with Juvenile Hepatic Cirrhosis and Alpha-1-Antitrypsin Defi-ciency. Biochem. Biophys. Acta 429:359.

7. Peters SP, Coyle P, Coffee CJ, Glew, RH, Kuhlenschmidt MS, Rosenfeld L, and Lee YC

(1977) Purification and Properties of a Heat-Stable Glucocerebroside Activating Factor from Control and Gaucher Spleen. J. Biol. Chem. 252:563.

8. Obrink B, Kuhlenschmidt MS, and Roseman S. (1977) Adhesive Specificity of Juvenile

Rat and Chicken Liver Cells and Membranes. Proc. Natl. Acad. Sci. USA 74:1077.

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9. Schnaar RL, Weigel PH, Kuhlenschmidt MS, Lee YC, and Roseman S. (1978) Adhesion of Chicken Hepatocytes to Polyacrylamide Gels Derivatized with N-acetylglucosamine. J. Biol. Chem. 253:7940.

10. Weigel PH, Schnaar RL, Kuhlenschmidt MS, Schmell E, Lee RT, Lee YC, and Roseman

S. (1979) Adhesion of Hepatocytes to Immobilized Sugars: A Threshold Phenomenon. J. Biol. Chem. 254:10830.

11. Slife CW, Kuhlenschmidt MS, and Roseman S. (1980) Interaction Between Hepatocytes

and Immobilized Sugar Derivatives. Journal of Biological Chemistry 255:1230-1241.

12. Kawaguchi K, Kuhlenschmidt MS, Roseman S, and Lee, YC (1980) Synthesis of Some Cluster Galactosides and Their Effect on the Hepatic Galactose-Binding System. Arch Biochem. Biophys. 205:388.

13. Slife CW, Kuhlenschmidt, MS, and Roseman S. (1980) Interaction Between Hepatocytes and Immobilized Sugar Derivatives. In: Communications of Liver Cells, eds., H. Popper, F. Gudat, L., Bianchi, and W. Reutter, p. 403.

14. Kawaguchi K, Kuhlenschmidt, MS, Roseman S, and Lee YC (1981) Differential Uptake of D-Galactosyl- and D-Glucosyl-neoglycoproteins by Isolated Hepatocytes. J. Biol. Chem. 256:2230.

15. Guarnaccia SP, Kuhlenschmidt MS., Slife CW, and Schnaar RL (1982) Hepatocyte Ad-

hesion to Immobilized Carbohydrates. II. Cellular Modification of the Carbohydrate Sur-face. J. Biol. Chem. 257:14293.

16. Kuhlenschmidt MS, Schmell E, Slife CW, Kuhlenschmidt TB, Sieber F, Lee YC, and

Roseman S. (1982) Studies on Intercellular Adhesion of Rat and Chicken Hepatocytes: Conditions Affecting Cell-Cell Specificity. J. Biol. Chem. 257:3157.

17. Kuhlenschmidt MS, Albanese J, Schmell E, Slife CW, and Roseman S. Studies on Inter-

cellular Adhesion of Rat and Chicken Hepatocytes: Demonstration of Tissue-Specific Adhe-sion in Mixtures of Hepatocytes and Heart Myocytes. J. Biol. Chem. 257:3165.

18. Schmell E, Slife CW, Kuhlenschmidt MS, and Roseman S. (1982) Studies on Intercellular

Adhesion of Rat and Chicken Hepatocytes: Conditions For Stimulation by Liver Plasma Membranes. J. Biol. Chem. 247:3171.

19. Kuhlenschmidt MS, Briggs, D, and Roseman S. (1982) Specific Chicken Hepatocyte Ad-

hesion Mediated by a Ganglioside Fraction from Plasma Membranes. Fed. Proc. 41:754. 20. Roseman S, Kuhlenschmidt MS, Slife CW, Zhou R, Park L, and Cox D. (1983) Studies

on Cell-Cell Adhesion. In: Structural Carbohydrates of the Liver, eds., H. Popper, W. Reut-ter, F. Gudat, and E. Kottgen, p. 237.

21. Kuhlenschmidt TB, Kuhlenschmidt MS, Roseman S, and Lee YC (1984) Binding and En-

docytosis of Glycoproteins by Isolated Chicken Hepatocytes. Biochemistry 23:6437. 22. Smith RD, Levy MG, Kuhlenschmidt MS, Adams JH, Rzechula DL, Hardt TA, and Kocan

KM (1986) Isolate of Anaplasma marginale not Transmitted by Ticks. Am. J. Vet. Res. 47:127.

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23. Adams, J. H., Smith, R. D., and Kuhlenschmidt, M. S. (1986) Identification of Common and Unique Antigens of Two Isolates of Anaplasma marginale Using Western Blots. Am. J. Vet. Res. 47:501.

24. Hosek, J., Todd, K., and Kuhlenschmidt, M. S. (1988) Improved Method for High-Yield

Excystation and Purification of Infective Sporozoites of Eimeria spp. J. Protozoology 35:534.

25. Hosek, J., Todd, K., and Kuhlenschmidt, M. S. (1988) Demonstration of Acid Phospha-

tase in Eimeria spp.: Partial Characterization of the Enzyme in E. vermiformis. J. Protozo-ology 35:524.

26. Hoffmann, W. E., Sanecki, R. K., Dorner, J. L., Rippy, M. K., Soeter, D. F., and

Kuhlenschmidt, M. S. (1988) Isoenzymes of Alkaline Phosphatase from Liver and Intes-tines of Dogs and Rabbits. In Animal Clinical Biochemistry (D. J. Blackmore, ed.), Cam-bridge University Press, pp. 181-187.

27. Hadley, S. P., Hoffmann, W. E., Kuhlenschmidt, M. S., Dorner, J. L., and Sanecki, R. K.

(1990) Effect of Glucocorticoids on Alkaline Phosphatase, Alkaline Phosphatase and Gamma-Glutamyltranspeptidase in Cultured Dog Hepatocytes. (Enzyme 43, 89-98.)

28. RK Sanecki, WE Hoffmann, JL Dorner and Kuhlenschmidt MS (1990) Purification and

comparison of corticosteroid-induced and intestinal isoenzymes of alkaline phosphatase in dogs. American Journal of Veterinary Research 51:1964-1968.

29. McLaughlin, G. L., Stimac, J. F., Luke, J. M., Kuhlenschmidt, M. S., Vemon, R. A., Meton,

D. L., Visvesvara, G. S., and Whiteley, H. E. (1990) Development of Acanthamoeba-cornea coincubation assays. Reviews in Infectious Diseases 13, 397-398.

30. Kuhlenschmidt, M. S., Hoffmann, W. E., Rippy, M. K., and Hadley, S. P. (1991) Isolation

and Characterization of Hepatocytes from Glucocorticoid-treated Dogs: Analysis of the Asialoglycoprotein Receptor. Biochem. Med. Met. Biol.46, 152-168.

31. Hooser, S. B., Beasley, V. R., Waite, L. L., Kuhlenschmidt, M. S., Carmichael, W. W., and

Haschek-Hock, W. M. (1991) Alterations in Rat Hepatocytes Induced In Vitro and In Vitro Microcystin-LR, a Hepatotoxin from the Blue-Green Alga, Microcystis aeurginosa. Vet. Pathol. 28, 259-266.

32. Sanecki, R. K., Hoffmann, W. E., Kuhlenschmidt, M. S., Felsburg, P., Hardy, M. R., Lee, Y.

C., and Dorner, J. L. (1991) Canine Corticosteroid Alkaline Phosphatase Isozyme: Purifi-cation and Comparison to the Intestinal Isozyme. Am. J. Vet. Res. 51, 1964-1968.

33. Gelberg, H., Whiteley, H., Ballard, G., Scott, J. and Kuhlenschmidt, M. S (1991) Temporal

Lectin Histochemical Characterization of Brush Border of Porcine Small Intestine. Am. J. Vet. Res. 53: 1873-1880.

34. Hooser, S. B., Kuhlenschmidt, M. S., Dahlem, A. M., Beasley, V. R., Carmichael, W. W.,

and Haschek, W. M. (1991) Hepatic Uptake and Subcellular Localization of Tritiated-Microcystin-LR in Perfused Rat Liver and Hepatocyte Suspensions. Toxicon. 29, 589-601.

35. Zhang, J., Kuhlenschmidt, M. S., and Dunn, F. (1991) Influences of Structural Factors of

Biological Media on the Acoustic Nonlinearity Parameter B/A. J. Acoust. Soc. Am. 89, 80-91.

27

36. Thulin, J. D., Kuhlenschmidt, M. S. and Gelberg, H. B. (1991),. An Intestinal Xenograft Model for Studying Enteropathogens. Lab Invest. 65, 719-731.

37. Gelberg H.B., Whiteley H., Ballard, G, Scott J, and Kuhlenschmidt, M.S. (1992) Temporal

Lectin Histochemical Characterization of Brush Border of Porcine Small Intestine. American Journal of Veterinary Research 53: 1873-1880.

38. Schleuter, A. J., Segre, D., Kuhlenschmidt, M. S., and Segre, M. (1992) Behavior of the

Idiotypic Network in a Conventional Immune Response. I. Kinectics of idiotypic and anti-idiotypic antibodies following immunization with T-independent and T-dependent antigens. Cellular Immunology 144:311-323.

39. Hayase, T., Rice, K. G., Dziegielewska, K. M., Reilly, T. and Kuhlenschmidt, M. S., and

Lee, Y. C. (1992). Comparison of N-Glycosides of Fetuins from Different Species and Hu-man a2-HS-Glycoprotein. Biochemistry 31:4915-4921.

40. Rolsma, M., Gelberg, H., and Kuhlenschmidt, M. S. 1994. Assay for Evaluation of Rota-

virus-Cell Interactions: Identification of an enterocyte ganglioside fraction that mediates Group A porcine rotavirus recognition. J. Virology 68:258-268.

41. Thulin, J. D., Kuhlenschmidt, M. S., Rolsma, M. D., Muelbroek, J., Current, W. L., and

Gelberg, H. B. 1994. An intestinal xenograft model for Cryptosporidium parvum infection. In-fection and Immunity 62:329-331.

42. Solter, P. F., Hoffmann, W. E., Chambers, M. D., Schaeffer, D. J. and Kuhlenschmidt, M.

S. (1994) Hepatic Tissue bile acids concentrations and enzyme activities in prednisone treated dogs Am. J. Vet. Res. 55:1086-1092.

43. Reilly, T. and Kuhlenschmidt, M. S. (1996) Characterization and sequencing of a respira-

tory burst-inhibiting acid phosphatase from Fransciella tularensis : Identification as a Puta-tive Virulence Factor J. Biol. Chem. 271: 10973-10983.

44. Kuhlenschmidt M.S., Rolsma M.D., Kuhlenschmidt T.B., Gelberg H.B. (1997) Characteriza-

tion of the Porcine Enterocyte Receptor for Group A Rotavirus. Adv. Exp. Med. Biol. 412: 135-143.

45. Gelberg H.B., Thulin J.D., Kuhlenschmidt M.S. (1997) Application of Intestinal Xenografts

to the Study of Enteropathogenic Infectious Disease. Adv. Exp. Med. Biol. 412:31-35. 46. Rolsma, M. D., Kuhlenschmidt, T. B., Gelberg, H.B. and Kuhlenschmidt, M. S. (1998) Structure and function of a porcine enterocyte ganglioside receptor for group a rotavirus. J. Virol. 72:9079-9091. 47. Kuhlenschmidt TB. Hanafin B and Kuhlenschmidt MS (1999) Sialic Dependence and Inde-

pendence of Group A Rotavirus. Adv. In Experimental Biology and Medicine 473:309-317. 48. Moreno B, Bailey BN, Luo S, Martin MB, Kuhlenschmidt M, Moreno SN, Docampo R, Old-

field E. 2001 (31)P NMR of apicomplexans and the effects of risedronate on Cryptosporidi-um parvum growth. Biochem Biophys Res Commun. 284:632-7.

49. Trask J, Kalita, PK., Kuhlenschmidt MS, Smith, RD. and Funk TL. 2004 Overland and

Near-surface Transport of Cryptosporidium parvum. J. Environ. Qual. 33:983-993.

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50. Johnson JK, Schmidt J., Gelberg HB and Kuhlenschmidt, MS (2004) Microbial Adhesion of Cryptosporidium parvum sporozoites: Purification of an Inhibitory Lipid from Bovine Mucosa. J. Parasitology 90:980-990.

51. Wetzel DM., Schmidt J, Kuhlenschmidt MS., Dubey JP, and Sibley LD (2005) Gliding mo-

tility by Cryptosporidium parvum sporozoites relies on a conserved actin-myosin motor. In-fection and Immunity, 73:5379-5387.

52. Johanna S. Salzer, Innocent B. Rwego, Tony L. Goldberg, Mark S. Kuhlenschmidt, and

Thomas R. Gillespie. Giardia sp. and Cryptosporidium sp. Infections in primates in distrubed and undisturbed forest in western Uganda. (2007) J. Parasitology 93:439-40.

53. A. Andres, S. M. Donovan, T. B. Kuhlenschmidt, and M. S. Kuhlenschmidt (2007)

Isoflavones at Concentrations Present in Soy Infant Formula Inhibit Rotavirus Infection in Vitro., J. Nutr. 137: 2068 - 2073.

54. Marıa Laura Salto, Theresa Kuhlenschmidt, Mark Kuhlenschmidt, Rosa M. de

Lederkremer, and Roberto Docampo (2008) Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi. Molecular & Biochemical Parasitology 158:120–130.

55. JoAnn Schmidt and Mark S. Kuhlenschmidt (2008) Microbial Adhesion of Cryptosporidium

parvum:Identification of a colostrum-derived inhibitory lipid. Molecular and Biochemical Parasitology 162:32-9.

56. M. F. Pineda, L. L. Chan, Theresa B. Kuhlenschmidt, Mark S. Kuhlenschmidt, and Brian.

T. Cunningham (2009) Rapid Label-Free Selective Detection of Porcine Rotavirus using Photonic Crystal Biosensors for Groundwater Monitoring. IEEE Sensors Journal 9:470-477

57. Liu Y, Janjaroen D, Kuhlenschmidt MS, Kuhlenschmidt TB, and Nguyen TH (2009) Depo-

sition of Cryptosporidium Parvum Oocysts on Natural Organic Matter Surfaces: Microscopic Evidence for Secondary Minimum Deposition in a Radial Stagnation Point Flow Cell. Langmuir 25:1594-1605.

58. Pineda MF, Chan LL, Kuhlenschmidt T, Kuhlenschmidt, MS. and Cunningham BT (2009)

Rapid Label-Free Selective Detection of Porcine Rotavirus using Photonic Crystal Biosen-sors. Sensors Journal, IEEE 9: 470-477.

59. Andres A, Donovan, SM and Kuhlenschmidt MS (2009) Isoflavones and Virus Infections

(Invited Review), J. Nutritional Biochemistry 20:563-569. 60. Donovan SM, Andres, A, Mathai, RA, Kuhlenschmidt TB, and Kuhlenschmidt MS (2009)

Soy Formula and Isoflavones and the Developing Intestine. Nutritional Reviews 67(suppl 2) S192-S200.

61. Gutierrez L, X Li, Wang J, Nangmenyi G, Economy J, Kuhlenschmidt TB, Kuhlenschmidt

MS, and Nguyen TH (2009) Adsorption of Rotavirus and bacteriophage MS2 using glass fi-ber coated with hematite nanoparticles. Water Res 43:5198-208.

62. Salzer JS, Rwego IB, Goldberg TL, Kuhlenschmidt MS, Gillespie TR. (2009) A legacy of

low impact logging does not elevate prevalence of potentially pathogenic protozoa in free-ranging chimpanzees and lowland gorillas in the Republic of Congo EcoHealth 6:557-64.

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63. Tang, X, Kuhlenschmidt TB, Zhou J, Bell P, Wang F, Saif TA, and Kuhlenschmidt MS,

Mechanical Force Affects Expression of an In Vitro Metastasis-Like Phenotype in HCT-8 Cells. (2010) Biophysical journal 99:2460-2469 (cover page article) www.biophysj.org/biophysj/supplemental/S0006-3495(10)01029-5.).

64. Janjaroen D, Liu Y, Kuhlenschmidt, MS, Kuhlenschmidt, TB, and Nguyen, TH. (2010)

Role of Divalent Cations on Deposition of Cyrptosporidum parvum Oocysts on Natural Or-ganic Matter Surfaces. Environ. Sci. Technol. 44:4519-24.

65. Liu Y, Kuhlenschmidt, MS, Kuhlenschmidt, TB, and Nguyen, TH. (2011) Composition and

Conformation of Cryptosporidium parvum Oocyst Wall Surface Macromolecules and Their Effect on Adhesion Kinetics of Oocysts on Quartz Surface. Biomacromolecules. 11:2109-15.

66. Bergner DW, Kuhlenschmidt TB, Firkins LD, and Kuhlenschmidt MS, (2011) Inhibition of

rotavirus infectivity by a neoglycolipid receptor mimetic" Nutrients 3:228-244. 67. Kowalewski MM, Salzer JS, Deutsch JC, M Raño,. Kuhlenschmidt MS, and Gillespie, TR.

(2011) Black and gold howler monkeys (Alouatta caraya) as sentinels of ecosystem health: patterns of zoonotic protozoa infection relative to degree of human–primate contact. Am. J. Primatology 73:75-83.

68. Perez VG, Jacobs CM, Barnes J, Jenkins, Kuhlenschmidt MS, Fahey GC, and Pettigrew J

(2011) Effect of corn distillers dried grains with solubles MC and Eimeria acervulina infec-tion on growth performance and the intestinal microbiota of young chicks. J. Poultry Sci. 9:959-964,

69. Bhattarai R, Kalita P, Trask J, Kuhlenschmidt MS (2011) Development of a physically-

based model for transport of Cryptosporidium parvum in overland flow. Environ Modell SoftW 26:1289-1287.

70. Tang X, Cappa T, Kuhlenschmidt TB, Kuhlenschmidt MS. and. Saif TA, “Specific and Non-

Specific Adhesion in Cancer Cells with Various Metastatic Potentials”, (Book Chapter), Mechanobiology of Cell-Cell and Cell-Matrix Interactions, Springer Science, 2011.

71. Liu Y, Zhang C, Hilper M, Kuhlenschmidt, MS, Kuhlenschmidt, TB, Nguyen, TH (2012) Transport of Cryptosporidium parvum oocysts in a silicon micromodel. Environ. Sci. Tech. 46(3):1471-9

72. Brothers M, Ho M, Maharjan R, Clemons, N, Bannai Y, Waites M, Faulkner M,

Kuhlenschmidt T, Kuhlenschmidt MS, Blanke S, Rienstra C, Wilson, Brenda (2012) Mem-brane interaction of Pasteurella multocida toxin involves sphingomyelin. FEBS Journal 23:4633-48.

73. Li M, Bauer LL, Chen X, Wang M, Kuhlenschmidt TB, Kuhlenschmidt MS, Fahey GC, Jr,

and Donovan SM (2012) Microbial Composition and In Vitro Oligosaccharides and Prebiot-ics Differ between Formula-Fed and Sow-Reared Piglets. J. Nutrition 142: 1–10.

74. Tang X, Wen Q, Kuhlenschmidt TB, Kuhlenschmidt MS, Janmey PA, Saif TA (2012)

Attenuation of Cell Mechanosensitivity in Colon Cancer Cells during In Vitro Metastasis.

30

PLOS ONE 7 (11), e50443 75. Fuller KL, Kuhlenschmidt TB, Kuhlenschmidt MS, Jimenez-Flores R, and Donovan SM.

(2013) Milk Fat Globule Membrane Isolated from Buttermilk or Cheese Whey and their Li-pid Component Inhibit Infectivity of Rotavirus In Vitro. J. Dairy Science 2013 96(6):3488-97

76. Hester S, Chen X, Li M, Monaco M, Comstock S, Kuhlenschmidt T, Kuhlenschmidt, MS

and Donovan S. (2013) Human milk oligosaccharides inhibit rotavirus infectivity in vitro and in acutely infected piglets. British Journal of Nutrition 110:1233-1242

77. Liu Y, Zhang C, Hu D, Kuhlenschmidt, MS, Kuhlenschmidt TB, Mylon SE, Kong R,

Bhargava R, Nguyen T H (2013) Role of collector alternating charged patches on transport of Cryptosporidium parvum oocysts in a patchwise charged heterogeneous micromodel. Environ Sci Technol 47 2670-8.

78. Davidson PC, Kuhlenschmidt TB, Rabin Bhattarai, Prasanta K. Kalita, Mark S.

Kuhlenschmidt (2013) Investigation of Rotavirus Survival in Different Soil Fractions and Temperature Conditions. Journal of Environmental Protection 4: 1-9

79. McLaughlin, SJ, Kalita, PK, and Kuhlenschmidt MS. (2013) Fate of Cryptosporidium

parvum oocysts within soil, water, and Plant environment. Journal of Environmental Man-agement 131: 121-128

80. Li M, Monaco MH, Wang M, Comstock SS, Kuhlenschmidt TB., Fahey Jr GC, Miller MJ,

Kuhlenschmidt MS, and Donovan SM (2013) Human milk oligosaccharides shorten the duration of rotavirus-induced diarrhea by modulating mucosal immune response and gut microbiota in piglets. ISME Journal (in press; http://dx.doi.org/10.1038/ismej.2014.10)

81. Davidson PC, Kuhlenschmidt TB, Bhattarai R,. Kalita PK, S. Kuhlenschmidt MS (2014) Ef-

fects of Soil Type and Cover Condition on Cryptosporidium parvum Transport in Overland Flow. Water, Air, & Soil Pollution 225:1882.

82. Xin Tang, Theresa B. Kuhlenschmidt, Qian Li, Shahjahan Ali, Stephane Lezmi, Hong Chen,

Melissa Alves, William W. Laegreid, Taher A. Saif, *, and Mark S. Kuhlenschmidt (2014) Mechanical Microenvironment Regulates Colon Cancer Metastasis. Molecular Cancer (in press)

83. Davidson, PC, Kuhlenschmidt TB, Bhattarai R, Kalita PK, and Kuhlenschmidt MS (2014)

Overland transport of rotavirus and the effect of soil type and vegetation, App Environ Micro (in review)

Manuscripts in Preparation JA Schmidt and MS Kuhlenschmidt. Inhibition of Cryptosporidium parvum sporozoite microneme secretion and host cell invasion by long-chain unsaturated fatty acids.

Jennifer H. Lee, Henrietta Awobode, Theresa B. Kuhlenschmidt, Milton M. McAllister and Mark S. Kuhlenschmidt. Long Chain Polyunsaturated Fatty Acids Inhibit Infectivity of Apicomplexa Parasites.