Culture Techniques

8/2/2019 Culture Techniques

1/17

Microbiology

Class Three

February 7-9

Culture Characteristics


2/17

Class schedule( Part One)

Check results from week 2.

Record results in templates.

Organisms studied

week 2 S. marcescens

B. subtilis

M. luteus E. coli


3/17

Culture characteristics

Use supplement and handout toobserve the growth of the fourorganisms in the slant, deep, broth, andon the plate.

Do the organisms look like one of theexamples on your sheet?

Try to record their appearance on yourtemplates


4/17

Culture observations on the agarplate

Color production( chromogenesis). Anexample of this is the pink color ofSerratia

Growth pattern and characteristics

Amount of growth( scant or heavy)


5/17

Comparison of E. coliand Micrococcusluteus


6/17

Colony morphology


7/17

Colony morphology


8/17

Margin of the colonies


9/17

Elevation


10/17

Broth culture( refer to supplement)

Cloudy

Turbid( Flocculent)

Sediment formation

Pellicle formation


11/17

Slants

Is there growth in thebottom ?

Is there growth on theslant itself

What are the growthcharacteristics on theslant?

Key words

AerobicAnaerobic

Facultative
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12/17

Isolation of Pure culture

Observe your dilution streak of your mixedculture

On the bottom of your Petri dish circle colonies

of two organisms Example

ML/SM mixture circle yellow and pink cultures

With your inoculating loop lift cells from circledcolonies and streak on new plate or inoculate aslant per detailed instructions in class
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13/17

Part One Completion of week 2work

When you have finished observing allof your cultures

Place all tubes in rack in hazardouswaste

Place all plates in cans in hazardouswaste

Wipe down desk top
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14/17

New work( supply table)

Eight Organisms for Study/Table

8 Plates

8 Deeps( if available) 8 Slants

8 Broths
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15/17

Preparation

Label all tubes and plates carefully

Assign each member of the group 2

organisms Transfer the organisms to the culture

media using aseptic techniques used inweeks one and two
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16/17

Organisms for study

Gram negative organisms PA - Pseudomonas aeruginosa PV- Proteus vulgaris EC- Escherichia coli EA- Enterobacter aerogenes

Gram Positive Organisms BS - Bacillus subtilis

SA - Staphylococcus aureus SE - Staphylococcus epidermidis SS- Streptococcus salivarius
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17/17

Review for Quiz One

1. Aseptic techniques

2. Culture transfer techniques3. Media

4. Safety precautions

5. Microscope identification
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Culture Techniques