CULTURE COLLEGE

Presented by:

Celeste Industries Corporation

BIOLOGICAL OPPORTUNITIES

forCLEANING

with CELESTE BIOZYME

Drain Maintenance

Lavatory Odor and Mildew Control

Biozyme II Mildewstat Property

Biozyme IITreated

Biozyme II Treated

Long Term Carpet Odor Control

Airline Opportunities for Biozyme

• Lavatories– Toilet shroud cleaning and odor control– Floors– Drain maintenance

• Cabin– Carpet cleaning additive for odor control

• Galley– Drain & Drain Mast maintenance

Celeste Biozyme provides a bio-film that continues to work after your employees are finished cleaning.

BASIC MICROBIOLOGY

How does Biozyme work?Enzyme function.Microbial life cycle

Bacteria – Enzyme Relationship

BACTERIUM

ENZYMES

BACTERIA, which are living single celled organisms, produce ENZYMES, which are non-living molecules.

Enzymes

Non-living molecules produced by bacteria

Biochemical catalysts

Break down organic waste

Specific activity

Enzyme Activity

Enzyme Substrates

Protease

StarchProtein Lipid (fat)

Enzyme Activity

Protease and

Protein

Protease and

Lipid (fat)

Protease and

Starch

Enzyme Activity

Protease and

Protein

Lipaseand

Lipid (fat)

Amylase and

Starch

Enzymes and Their Substrates

Protease – protein Urease – urea

Amylase – starch

Lipase – fat

Esterase – fat

Xylanase – plant material

Cellulase – cellulose

Protease

Cellulase

Xylanase

Amylase

Lipase

Esterase

Naturally Occurring Bacteria

Single celled, living organisms

Consume organic waste

Found virtually everywhere

Prolific enzyme production

Requirements for Bacterial Activity

Water

Nutrients (organic waste)

Time

Correct environmental conditions

Essential Bacterial Profile

Reproduce quickly

Safe, non-pathogenic, naturally occurring

Form spores for long term stability

High level production of multiple enzymes

Function in or under diverse environmental conditions

The type of bacteria used in Celeste Biozyme

Bacillus Vegetative Cells

Enzyme producers

Consume organic waste

Grow logarithmically

Mobile

Form spores when conditions dictate dormancy is appropriate

Bacillus Spores (Dormant Cells)

Heat resistant

Chemical resistant

Desiccation resistant

UV resistant

Germinate to vegetative cells when environmental conditions are right

Bacillus Lifecycle

VEGETATIVE CELL VEGETATIVE CELL WITH SPORE

FREE SPORE

Spore Formation

VegetativeCell Lysis

SporeGermination

Bacillus Waste Digestion Analogy

Bacterium Enzymes

Enzymes break down waste into

small pieces

CO2 H2O

Bacterium digests waste and release

CO2 and H2O

Pizza (organic waste)

Logarithmic Growth

Growth Curve

PopulationPopulation

TimeTime

Bacterial Respiration

Aerobic - Oxygen required

Anaerobic - Oxygen not required

Facultative Anaerobic - Grows with or without oxygen

Celeste Biozyme is functional with or without oxygen.

Functional Temperature and pH Ranges

41° - 131° F (5° - 55° C)

pH 4.5 - 10

Celeste Biozyme is functional in a wide range of temperature and pH

Complimentary Activity of Celeste Biozyme Bacteria

ATTRIBUTES BACTERIA

Strain 1 Strain 2 Strain 3

pH 5 No No Yes

pH 10 Yes Yes No

55° C Yes Yes No

Anaerobic Yes Yes No

Protease Fair Good Excellent

Lipase Excellent Good Fair

Amylase Good Good Excellent