CUKIERMAN, Edna

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CURRICULUM VITAE Date: June, 2018

NAME: Edna Cukierman

CONTACT: Cancer Biology Fox Chase Cancer Center 333 Cottman Ave. Room W428 Philadelphia, PA. 19111. edna.cukierman@fccc.edu Tel: 215-214-4218 Web: http://www.fccc.edu/research/pid/cukierman/index.html Twitter: @ednacukierman CITIZENSHIP: USA & México EDUCATION: Technion-Israel Institute of Technology, B.S., Biology, 1991 Technion-Israel Institute of Technology. M.Sc., Biochemistry

(under the mentorships of Drs. Dan Zilberstein and Dan Cassel) 1993 Technion-Israel Institute of Technology. D.Sc. (Ph.D.)., Molecular and Cell Biology (under the mentorship of Dr. Dan Cassel) 1997 PROFESSIONAL EXPERIENCE: Teaching Assistant at the Technion-Israel Institute of Technology 1991-1997 Postdoctoral Fellow, NIH/National Institute of Dental and Craniofacial Research, 1997-2002 Craniofacial Developmental Biology and Regeneration Branch (Mentor- Dr. Kenneth M. Yamada) Assistant Professor (Associate Member), Institute for Cancer Research, 2002-2009 Fox Chase Cancer Center, Philadelphia, PA Adjunct Associate Professor, Drexel University School of Medicine, 2008-date

Philadelphia, PA Associate Professor (Member) with Tenure, Institute for Cancer Research, 2009-date

Fox Chase Cancer Center, Philadelphia, PA Adjunct Associate Professor, Faculty of Health Sciences 2009-date

Ben-Gurion University of the Negev in Israel. Faculty Affiliate Member, Institute for Translational Medicine and Therapeutics 2012-date Clinical and Translational Research Center/Perelman School of Medicine, UPenn Adjunct Associate Professor, Faculty in the Bioengineering Department 2012-date Temple University College of Engineering. Philadelphia, PA Interim Co-director of the FCCC/Drexel University graduate programs 2017-2018

in Cancer Biology, MCBG, and Biochemistry. Philadelphia, PA

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Adjunct Associate Professor in the Lewis Katz School of Medicine 2017-date at Temple University. Philadelphia, PA Co-Leader of Marvin & Concetta Greenberg Pancreatic Cancer Institute 2017-date

Fox Chase Cancer Center. Philadelphia, PA Co-Director of Immune Monitoring Facility 2018-date

Fox Chase Cancer Center. Philadelphia, PA FELLOWSHIPS, HONORS, AWARDS: The Dean’s List of the Faculty of Biology (Technion Institute of Technology) 1989-1990 The Dean’s List of the Faculty of Biology (Technion Institute of Technology) 1990-1991 Fellowship from the government of Israel 1988-1991 Feldman Fellowship 1990-1993 Fund of the Technion Institute of Technology Fellowship 1993-1997 Fogarty International Center, Fellowship for postdoctoral training at the NIH 1997-2002 Fellows Award for Research Excellence at the NIH 2002 National Pancreas Foundation Award 2004-2005 AACR Career Development Award 2004-2006 Nikon Small World Competition (Image Distinction Award) 2005 W.W. Smith Charitable Trust Award 2005-2008 Olympus BioScapes Digital Imaging Competition (Honorable Mention Award) 2006 AACR Career Development Award (continuation from 2004) 2006-2007 Temple-FCCC Nodal Award 2013-2015 Member of NIH/NCI’s Tumor Progression and Metastasis Study Section 2013-2019 Image featured at Dulles and PLH Airports (“Life Magnified” by NIH/ASCB) 2014 DOD Idea Award with Special Focus in Pancreatic Cancer 2015-2017 NCI Cancer Close Up Project, Pancreatic Cancer Image, for AACR and ASCO 2016 NCI Cancer Close Up Project, Lung Cancer Image, online showing 2016 Distinguished Achievement Award in Cancer Research and Clinical Management 2016 -Pancreas Focus- by the Chinese Society of Clinical Oncology (Chinese’s ASCO) Translational Clinical Protocol Development Award (FCCC) 2018 PanCAN’s Honorary Co-Chair of PurpleStride Philadelphia. 2018 SOCIETIES: The American Society for Cell Biology 2000-date American Association for Cancer Research 2004-date International Cancer Microenvironment Society 2007-2010 American Gastroenterological Association 2010-date Metastasis Research Society 2018-date ADVISORY BOARDS / REVIEW PANELS and TEACHING: Israel Science Foundation Academic and Executive Boards 2005-date Scientific Reviewer. (ad hoc). DOD’s Breast Cancer Research Program, Scientific Reviewer 2006-2009 New and Synergistic Idea grants, Cell Biology-2 panel. (Member). DOD’s Breast Cancer Research Program, Scientific Reviewer 2007 Concept Idea grants. (ad hoc).

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North Carolina Biotechnology Center, Scientific Reviewer, 2007 Biotechnology Research Grant Program. (ad hoc). ACS’s Cell Structure and Metastasis Committee, Scientific Reviewer 2007-2016 (Permanent Member since January 2010). Drexel University College of Medicine, Ph.D. Program 2007-2010 Advisor in the Faculty Committee; Keneshia Turner (Dr. Reginato, mentor). NIH/NCI’s Tumor Micro Environment Study Section, Scientific Reviewer 2008 (ad hoc). NIH’s Diversity Pre-doctoral Fellowship Study Section, Scientific Reviewer 2008 (ad hoc). Graduate course at Drexel; Cancer Biology, 2008-2012 lecture in Tumor Microenvironment Graduate course at Temple; Molecular, Cellular and Systems Signal Transduction 2008-date (course #: BMSC 8206). Lectures in “signaling by integrins and non-receptor tyrosine kinases”

and in “actin dynamics and small GTPAse signaling” since 2014. Canadian Breast Cancer Foundation – Ontario, Canada 2009 Panel for Research Project Grant Programs, Scientific Reviewer. (ad hoc). DOD’s Ovarian Cancer Research Program OC-3, Scientific Reviewer 2009 (ad hoc) Hebrew University Jerusalem Israel, Ph.D. Program. 2009 Graduate Thesis Reviewer; Nilly Shimony (Drs Panet and Haviv, mentors). NIH/NCI’s F09 Fellowship Panel Study Section, Scientific Reviewer 2009 (ad hoc) NIH/NCI’s Tumor Progression and Metastasis Study Section, Scientific Reviewer 2009-2010 (ad hoc) AACR’s Tumor Microenvironment Subcommittee/ Tumor Biology Section 2009-2010 Annual Meeting 2010 Program Committee (Member) Swiss Federal Institute of Technology Zurich or ETH, Ph.D Program 2009-2011 Department of Materials, co-supervisor; Maria Håkanson (M. Textor, supervisor). Italian Association for Cancer Research, Preferred Reviewer 2009-date (Member) Author-in-Chief to Springer Reference Live: Cancer 2009-date Encyclopedia of Cancer University of Pennsylvania, School of Medicine, Ph.D. Program 2009-2012 Advisor in the Faculty Committee; Gabrielle. Wong (Dr. Rustgi, mentor). DOD’s Breast Cancer Review Panel/Concept Awards MBG-3, Scientific Reviewer 2010 (ad hoc) DOD’s Breast Cancer Research Program, Scientific Reviewer 2010-2011 "Functional Study of Biological Molecules," Idea Awards (CBY#5,2, ad hoc). Florida Department of Health, Scientific Reviewer (Member) 2010-date James & Esther King Biomedical and Bankhead-Coley Cancer Research Program Susan G. Komen for the Cure Research Programs, Scientific Reviewer 2011 Post-doctoral Fellowship-Basic Research (PDF-BR). NIH Small Business: Biomaterials, Delivery Systems, and Nanotechnology 2011

[IMST-13] Study Section, Scientific Reviewer (ad hoc). NIH/NCI’s Oncology I - Basic Translational (OBT) IRG Online 2011 Special Emphasis Panel, Cancer Biology/Signaling, Scientific Reviewer (ad hoc). The Breast Cancer Campaign, Scientific Reviewer (ad hoc) PhD grant applications 2011

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Louisiana Board of Regents Support Fund (RCS; Mail Reviewer) 2011 NIH/NCI’s Tumor Progression and Metastasis Study Section (Feb & Oct meetings), 2012 Scientific Reviewer (ad hoc) NSF-NCI Physical and Engineering Sciences in Oncology panel 2012 Scientific Reviewer (ad hoc) Roswell Park Cancer Institute, Ph.D. Program 2012 External Reviewer in the Thesis Defense of Anna Bianchi-Smiraglia (Dr. Bakin, mentor). Aberrant Pathways session/Pacific Symposium on Biocomputing 2013 (mail ad hoc) 2012 University of Pennsylvania, Cell & Molecular Biology Ph.D. Program 2012-2016 Advisor in the Faculty Committee; Bridget Sackey (Dr. Rebecca Wells, mentor). New Jersey Commission on Cancer Research-NJCCR, pre- and postdoctoral awards 2013 Scientific Reviewer NIH/NCI Special Emphasis Panel/Provocative Questions (March meeting), 2013 Group C - Cancer detection, diagnosis, and prognosis - Scientific Reviewer American Association for the Advancement of Science - Research Competitiveness 2013 Program - University of Kansas Medical Center’s Office of Scholarly, Academic

and Research Mentoring. Scientific Reviewer NIH/NCI’s Tumor Progression and Metastasis Study Section, 2013-2019 Scientific Reviewer (Member) American Urological Association (AUA) Research Scholar Grants Program 2013-2014

Scientific Reviewer Temple University – Bioengineering at the College of Engineering, 2013-2016 Ph.D. Program Advisor in the Faculty Committee; Seda Karamil (Dr. P Lelkes, mentor). Oak Ridge Associated Universities - The University of Virginia Cancer Center 2014 Team Science Awards. Scientific Reviewer North Dakota’s Research ND - North Dakota's Research Universities and companies 2014 Grant Proposal. Scientific Reviewer NIH/NCI Special Emphasis Panel/Provocative Questions (May meeting), 2014 Mechanisms of Tumor Development or Recurrence, Cancer Therapy and Outcomes, and Clinical Effectiveness.- Scientific Reviewer (ad hoc) NIH/NCI Physical Sciences Oncology Centers & Division of Cancer Biology. 2014 - U54 - Center of Excellence Grants. Scientific Reviewer (ad hoc) University of Pennsylvania Teaching “Cancer (and similar)” topic 2014-date in Course "The ECM ,Adhesion Receptor Signaling, and Translational Biomechanics" directed by Drs. Wells and Assoian (in 2014) and Mauck (starting in 2015) . NIH/NCI Physical Sciences Oncology Projects -UO1- Scientific Reviewer (ad hoc) 2015 Drexel University, Teaching “Advance Cell Biology” Course, 2015-date Topic "Cell-ECM Interactions" directed by Dr. Michael Bouchard. University of Pennsylvania, Pharmacology Ph.D. Program 2015-date Advisor in the Faculty/Thesis Committee; Diana Avery (Dr. Ellen Pure, mentor). Drexel University, College of Medicine MD/Ph.D Program 2015-2016 Advisor in the Faculty/Thesis Committee; Kristina Shahriari (Dr. A Fatatis, mentor). Temple University Department of Bio-Engineering 2015-2018 External Advisor Board Member U.S.-Israel Binational Science Foundation – Scientific Reviewer (ad hoc) 2016 Memorial Sloan Kettering Cancer Center –U54. Scientific Reviewer (ad hoc) 2016 Drexel University College of Medicine, M.Sc. Cancer Biology Program 2016 Advisor in the Faculty/Thesis Committee; Meghan Kopp (Dr. Golemis, mentor).

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University of Strasbourg, Institute of Genetics and Molecular and Cellular Biology 2016 Strasbourg, France. Laboratory of Cell Physics -Ph.D. Program Advisor/Jury in Thesis Defense Committee; Amélie Godeau (Dr. Riveline, mentor). Memorial Sloan Kettering Cancer Center; External Reviewer of U54 applications 2016 Singapore Ministry of Education, Scientific Reviewer 2016 for the Academic Research Fund, (multi PI grants) Oak Ridge Associated Universities - Florida Department of Health, 2016 Grant

Scientific Reviewer (ad hoc). Drexel University, College of Medicine Ph.D Program 2016-2017 Advisor in the Faculty/Thesis Committee; Erica Dalla (Drs. Fatatis & Meucci, co-mentors). NIH/NCI Consortium for Pancreatic Ductal Adenocarcinoma Translational 2017 studies on the Tumor Microenvironment -UO1- Scientific Reviewer (ad hoc) Genesis Oncology Trust of New Zealand dedicated cancer research funding 2017 Grant Scientific Reviewer (ad hoc) NIH/NCI Special Emphasis Panel (REP) of Neural Regulation of Cancer 2017 ZRG1OBT-S(55) Grant Scientific Reviewer (ad hoc), November 2017 meeting Drexel University, College of Medicine Ph.D Program 2017-date Advisor in the Faculty/Thesis Committee; Asurayya Worrede-Mahdi (Dr. Fatatis, mentor). Drexel University, College of Medicine Ph.D Program 2017-date Advisor in the Faculty/Thesis Committee; Matthew Scott (Drs. P Campbell mentor). Tenure and Promotions Committee; Bio-Engineering Department Temple University 2017-date Temple University, Lewis Katz School of Medicine Ph.D Program 2017-date Advisor in the Faculty/Thesis Committee; Sophie Lazar (Dr. L Glodfinger) Drexel University Graduate Course; Cancer Biology, 2018-date lecture in Tumor Microenvironment with an emphasis on Pancreatic Cancer Fox Chase Activities/Committees Tumor Cell Biology Working Group (Member) 2002-2009 Chaired by J. Chernoff Signal Transduction Interest Group (Member) 2002-2017 Initiative of Dr. E. Golemis Faculty Committee Oversight of Russian Graduate Student 2003-2007 Nina Makhortova Breast Cancer Research Program (Collaborating Member) 2003-2012 Chaired by Dr. L. Goldstein Pancreatic Cancer Interest Group (Member) 2003-2014 Initiative of Dr. N. Meropol and S. Cohen Cell Imaging Facility, Oversight Committee 2004-2010 (Member) Faculty Committee Oversight of Russian Graduate Student 2004 Yuliya V. Skorobogatko FCCC-Russian State Medical University Sister Institution Program, 2004-date (Member) 10th Annual Postdoctoral Day (Organizer) 2005 Faculty Committee Oversight of Russian Graduate Student 2006-2008 Andrey Parkhitko.

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Ovarian Cancer Research Program (Collaborating Member) 2006-2012 Chaired by Drs. T. Hamilton and A. Godwin. Junior Faculty at FCCC Advisor Committee (Adjunct Member) 2007-2008 Initiative of Dr. E. Golemis Ben Gurion University Collaborative Effort (participant) 2007-2009 Keystone Program in Personalized Kidney Cancer Therapy (Member) 2008-date Chaired by Drs. Hudes, Testa and Uzzo Institutional Biosafety Committee, (Member) 2008-2011 Gynecologic Cancer Therapeutic Trials (GCTT) Working Group (Member) 2008-date Cancer Biology Program (Primary Member) 2009-date Chaired by Drs. J. Chernoff and J. Testa. Molecular Translational Medicine Program (Secondary Member) 2009-2011 Chaired by Drs. E. Golemis, R. Cohen, and G. Adams. Women's Cancer Program (Secondary Member) 2009-2012 Chaired by Drs. L. Goldstein and M. Cristofanilli. Ben Gurion University Collaborative Effort (Co-organizing Member at FCCC) 2009-date Community College of Philadelphia Collaborative Effort 2010-date Safety Committee (Member) 2011-2013 Chaired by J. Rawson Institutional Bio-safety Committee (Chair) 2011-date Lincoln University – Fox Chase Partnership in Cancer Research and Training (P20) 2012-2013 (Committee Member) Co-mentor (together with Massimo Cristofanilli). Graduate Student 2012-2013 Xiaoshen Dong Mentor. Graduate Student (Master). Russian National Research Medical University 2012-2013 Alexander Zenin Safety Committee (ad hoc) 2013-2015 Chaired by J. Rawson (Daniel Rudolph/chair since 2014) Women Scientists and Physicians Leadership Roles - Interest Group (Member) 2013-date Faculty Committee Oversight of Russian Graduate Student 2013-date Oxana Dmitrieva (Sergei Grivennikov mentor) Committee to Assist and Advice Junior Faculty at FCCC (Member) 2014-2016 Faculty Senate Representative of Cancer Biology Department/Group 2014 Pancreatic Cancer Interest Group at FCCC (co-Leader) 2014-date Cell Imaging Facility, Oversight Committee (Member) 2015-date Early Phase Clinical Research Support Review Committee 2015-date Mentor. Graduate Student (Master). Drexel University. Dustin Rollins 2015-2016 Mentor. Graduate Student (PhD candidate). Drexel University. Jennifer Alexander 2015-date Faculty Committee Oversight of Russian Graduate Student 2016-date Marya Bondarenko Kozinova (Lori Rink mentor) Cancer Biology Faculty Search Committee (Chair) 2016 Translational Medicine Faculty Search Committee (Member) 2016-date Faculty Committee Oversight of Russian Graduate Student 2016-date Alena Klochkova (Igor Astsaturov mentor) Mentor. Graduate Student (PhD candidate). Drexel University. Kris Raghavan 2016-date Cancer Biology Scientific Retreat (Co-organizer) 2017 Faculty Committee Undergraduate Summer Research Fellowship Competition 2017 Cancer Center Support Grant, CCSG, supported Pilot Project Review Committee 2017

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Faculty Committee Oversight of Graduate Student from China 2017-date Peishan Zhang (Erica Golemis mentor; collaboration with a Chinese institution) AD HOC REVIEWER FOR: Acta Biomaterialia. Advanced Drug Delivery Reviews. American Journal of Pathology. Bio-macromolecules. Bio-materials. Bio-Techniques. BMC Cancer. Cancer Biomarkers. Cancer Cell International. Cancer Microenvironment. Cancer Research. Cancer Treatment Reviews. Carcinogenesis. Cell Adhesion & Migration. Cell Biochemistry and Biophysics. Cell Repports. Cellular and Molecular Biology. Clinical Cancer Research. Critical Reviews in Oncology/Hematology. Current Cancer Drug Targets. Current Drug Delivery. Current Opinion in Cell Biology. eLife. European Journal of Biochemistry. Experimental Cancer research. Experimental Cell Research. Faculty 1000. Food and Bioproducts Processing. Interface Focus. Integrative Biology. International Journal of Cancer. International Journal of Molecular Sciences. Journal of Anatomy. Journal of Biological Chemistry. Journal of Cell Biology. Journal of Cell Science. Journal of Clinical Investigation. Journal of Controlled Release. Journal of Cytotechnology. Journal of Mammary Gland Biology and Neoplasia. Journal of Molecular, Cellular, and Biochemical Toxicology. Matrix Biology. Molecular Biology of the Cell. Molecular Cancer. Molecular Cancer Research. Molecular Carcinogenesis. Molecular Cell Biology. Nanomedicine. Nature Cell Biology. Nature Materials. Nature Methods. Nature Protocols. Physical Biology (Physical Oncology). Physiological Genomics. PLoS ONE. PNAS. Regenerative Medicine. Trends in Biotechnology. Science. EDITORIAL: PLoS ONE Academic Editor 2012-date Physiological Genomics Editorial Board Member 2013-2015 Special Issue Tumor Engineering/Journal Advanced Drug Delivery Reviews 2014 Invited Co-Scientific Editor together with Professor Dietmar W. Hutmacher Tumor & Microenvironment Editorial Board Member 2015-date Matrix Biology Editorial Board Member 2018-date CONSULTING: NIH’s development of the first artificial Salivary Gland 1997-2003 Consultant in Cell Biology.

DROIA Oncology Ventures. Scientific consultant services on possible 2016 development of oncology therapies and clinical trials (emphasis in pancreas).

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RESEARCH INTERESTS: The research interests of my laboratory focus on studies of the implication of the natural fibroblastic 3D microenvironment progression due to tumor development in a bi-directional exchange of information at cell-matrix contacts such as “three-dimensional matrix adhesions” also known as ‘3D-adhesions.’ For this, I have developed a primary fibroblast-derived and in vivo-like 3D system that mimics stroma human progression (i.e., desmoplasia) in assorted epithelial cancers such as pancreas, kidney, inflammatory and regular breast, as well as ovary and lung. We use this physiologically relevant 3D system to investigate the mechanisms of matrix induced myofibroblastic differentiation (e.g., desmoplastic activation) and the tumorigenic effects imparted by tumor-associated extracellular matrices (i.e., tumor cell invasion, growth, etc) as well as its effects in neo-neurogenesis and perineural cancer cell invasion. The physiological/pathological relevant progressive stroma 3D system also serves as basis for a platform investigating tumor-associated matrix induced drug responsiveness and discovery of activated stromal (including neural) and cancer biomarkers. Tissue engineering methodologies are used to question the topographical/architectural vs. biochemical aspects of tumor-associated stromal microenvironments. Novel pre-clinical therapeutic approaches are assessed using human implanted surgical tissues in immune-compromised mice as well as via orthotopic and isogenic murine xenograft and spontaneous genetic models. Validations are conducted using simultaneous multi-channel spectral immunofluorescence using human surgical tissue microarrays that include well-annotated clinical information and patient outcomes; we developed special analysis software needed for this approach https://github.com/cukie/SMIA-CUKIE. Studies incorporate molecular biology, biochemical and cell-based assays, as well as laser scanning confocal immunofluorescence, real time multi-channel microscopy, digital imaging analyses, genetic manipulations, tissue patterning, mathematical modeling and more. PUBLICATIONS: Peer-Reviewed Publications Cassel, D., Shouvi, S., Glusman, G., Cukierman, E., Rotman, M., Zilberstein, D. Leishmania

donovani: characterization of a 38 kDa membrane protein that cross-reacts with the mammalian G-protein transducin. Exp. Parasitol. 72:411-417, 1991.

Makler, V., Cukierman, E., Rotman, M., Admon, A., Cassel, D. ARF-directed GTPase activating protein: purification and partial characterization. J. Biol. Chem. 270:5232-5237, 1995.

Cukierman, E., Huber, I., Rotman, M., Cassel, D. The ARF GTPase activating protein: Zinc finger motif and Golgi complex localization. Science. 270:1999-2001, 1995.

Poon, P., Wang, X., Rotman, M., Huber, I., Cukierman, E., Cassel, D., Singer, R.A., Johnston, G.C. Saccharomyces cerevisiae Gcs1 is an ADP-ribosylation factor GTPase-activating protein. Proc. Natl. Acad. Sci. 93:10074-10077, 1996.

Aoe, T., Cukierman, E., Lee, A., Cassel, D., Peters, P. J., Hsu, V. W. The KDEL receptor, ERD2, regulates membrane traffic by recruiting a GTPase-activating protein for ARF1. EMBO J. 16:7305-7316, 1997.

*Huber, I., *Cukierman, E., Rotman, M., Aoe, T., Hsu, V. W., Cassel, D. Requirement for both the amino-terminal catalytic domain and a noncatalytic domain for in vivo activity of ADP-ribosylation factor GTPase-activating protein. J. Biol. Chem. 273:24786-24791, 1998 (*Contributed equally).

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Wang, S., Cukierman, E., Swaim, W.D., Yamada, K.M., Baum, B.J. Extracellular matrix protein-induced changes in human salivary epithelial cell organization and proliferation on a model biological substratum. Biomaterials 20:1043-1049, 1999.

Aframian, D.J., Cukierman, E., Nikolovski, J., Mooney, D.J., Yamada, K.M., Baum, B.J. The growth and morphological behavior of salivary epithelial cells on matrix protein-coated biodegradable substrata. Tissue Eng. 6:209-216, 2000. (

Pankov, R., Cukierman, E., Katz, B.-Z., Matsumoto, K., Lin, D.C., Lin, S., Hahn, C., Yamada, K.M. Integrin dynamics and matrix assembly: Tensin-dependent translocation of α5β1 integrins promotes early fibronectin fibrillogenesis. J. Cell Biol. 148:1075-1090, 2000. (Cover article)

Aframian, D.J., Zheng, C., Goldsmith, C.M., Nikolovski, J., Cukierman, E., Yamada, K.M., Mooney, D.J., Birkedal-Hansen, H., Baum, B. J. Using HSV-thymidine kinase for safety in an allogeneic salivary graft cell line. Tissue Eng. 7:405-413, 2001.

Cukierman, E., Pankov, R., Stevens, D.R., Yamada, K.M. Taking cell-matrix adhesions to the third dimension. Science. 294:1708-1712, 2001. (Featured in Faculty 1000)

Aframian, D.J., Redman, R.S., Yamano, S., Nikolovski, J., Cukierman, E., Yamada, K.M., Kriete, M.F., Swaim, W.D., Mooney, D.J., Baum, B.J. Tissue compatibility of two biodegradable tubular scaffolds implanted beneath the skin of buccal mucosa in mice. Tissue Eng. 8:649-659, 2002.

Aframian, D.J., Tran, S.D., Cukierman, E., Yamada, K.M., Baum, B.J. Absence of tight junction formation in an allogeneic graft cell line used for developing an artificial salivary gland. Tissue Eng. 8:871-878, 2002.

Engelholm, L. H., List, K., Netzel-Arnett, S., Cukierman, E., Mitola, D.J., Aaronson, H., Kjøller, L., Jørgen K. L., Yamada, K. M., Strickland, D. K., Holmbeck, K., Danø, K., Birkedal-Hansen, H., Behrendt, N., Bugge, T. H. uPARAP/Endo180 is essential for cellular uptake of collagen and promotes fibroblast collagen adhesion. J. Cell Biol. 160:1009-1015, 2003.

Pankov, R., Cukierman, E., Clark, K., Matsumoto, K., Hahn, C., LaFlamme, S. E., Poulin, B., Yamada, K. M. Specific β1 integrin site selectively regulates Akt/PKB signaling via local activation of PP2A. J. Biol. Chem. 278:18671-18681, 2003.

Zajaczkowski, M.B., Cukierman, E., Galbraith, C.G., Yamada, K.M. Cell-matrix adhesions on poly(vinyl alcohol) hydrogels. Tissue Eng. 9:525-33, 2003.

Katz, B.-Z., Romer, L., Miyamoto, S., Volberg, T., Matsumoto K., Cukierman, E., Geiger, B., and Yamada, K. M. Targeting membrane-localized FAK to focal adhesions: Roles of tyrosine phosphorylation and Src family kinases. J. Biol. Chem. 278:29115-29120, 2003.

Einarson, M.B., Cukierman, E., Compton, D.A., Golemis, E.A. Human enhancer of invasion-cluster, a coiled-coil protein required for passage through mitosis. Mol. Cell Biol. 24:3957-3971, 2004. (Cover article)

Amatangelo, M.D., Bassi, D.E., Klein-Szanto, A.J., Cukierman, E. Stroma-derived 3D matrices are necessary and sufficient to promote desmoplastic differentiation of normal fibroblasts. Am. J. Pathol. 167:475-488, 2005. (Featured in Faculty 1000)

Johnson, J.E., Varkonyi, R.J., Schwalm, J., Cragle, R., Klein-Szanto, A., Patchefsky, A., Cukierman, E., von Mehren, M., Broccoli, D. Multiple mechanisms of telomere maintenance exist in liposarcomas. Clin. Can. Res., 11:5347-5355, 2005.

Bassi, D.E., Lopez DeCicco, R., Cenna, J., Litwin, S., Cukierman, E., Klein-Szanto, A.J. PACE4 expression in mouse basal keratinocytes results in basement membrane disruption and acceleration of tumor progression. Cancer Res. 65:7310-7319, 2005.

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Pankov, R., Endo, Y., Even-Ram, S., Araki, M., Clark, K., Cukierman, E., Matsumoto, K., Yamada, K.M. A Rac switch regulates random versus directionally persistent cell migration. J. Cell Biol. 170:793-802, 2005. (Cover article and Featured in Faculty 1000)

Okawa, T., Michaylira, C. Z., Kalabis, J., Stairs, D. B., Nakagawa, H., Andl, C., Johnstone, C. N., Klein-Szanto, A. J., El-Deiry, W. S., Cukierman, E, Herlyn M, Rustgi AK. The functional interplay between EGFR overexpression, hTERT activation and p53 mutation in esophageal epithelial cells with activation of stromal fibroblasts induce tumor development, invasion and differentiation. Genes Dev., 21:2788-2803, 2007. PMCID: PMC2045132

Damianova, R., Stefanova, N. Cukierman, E. Momchilova, A. Pankov, R. Three-dimensional matrix induces sustained activation of ERK1/2 via Src/Ras/Raf signaling pathway. Cell Biol. Int., 32:229-234, 2008. (Accepted prior to April 2007)

Quiros, RM., Valianou, M., Kwon, Y., Brown, KM., Godwin, AK., and Cukierman, E. Ovarian normal and tumor-associated fibroblasts retain in vivo stromal characteristics in a 3-D matrix-dependent manner. Gynecologic Oncology., 110:99-109, 2008. PMCID: PMC2612536

Serebriiskii, I., Castelló-Cros, R., Lamb, A., Golemis, EA., Cukierman, E: Fibroblast-derived 3D matrix differentially regulates the growth and drug-responsiveness of human cancer cells. Matrix Biol., 27:573-585, 2008. PMCID: PMC2603546

Castelló-Cros R, Khan DR, Simons J, Valianou M, Cukierman E: Staged stromal extracellular 3D matrices differentially regulate breast cancer cell responses through PI3K and beta1-integrins. BMC Cancer, 9:94, 2009. PMCID: PMC2669806.

Bassi DE, Zhang J, Cenna J, Litwin S, Cukierman E, Klein-Szanto A: Proprotein Convertase Inhibition Results in Decreased Skin Cell Proliferation, Tumorigenesis, and Metastasis. Neoplasia 12(7):516-26, 2010. PMCID: PMC2907578.

Goicoechea SM, Bednarski B, Stack C, Cowan DW, Volmar K, Thorne L, Cukierman E, Rustgi AK, Brentnall T, Hwang RF et al: Isoform-specific upregulation of palladin in human and murine pancreas tumors. PLoS ONE 5(4):e10347. 2010. PMCID: PMC2859948.

Shatalova E, Klein-Szanto A, Devarajan K, Cukierman E, Clapper ML: Estrogen and Cytochrome P450 1B1 Contribute to Both Early- and Late-Stage Head and Neck Carcinogenesis, Cancer Prev Res 4(1):107-15. 2011. PMCID: PMC3043603.

Kwon Y, Cukierman E* and Godwin AK*: Differential Expression of Adhesive Molecules and Proteases Define Mechanisms of Ovarian Tumor Cell Matrix Penetration/Invasion. PLoS ONE 6(4):e18872. 2011 PMCID: PMC3079735 (*co-corresponding author).

Lee H-O, Mullins SR, Franco-Barraza J, Valianou M, Cukierman E*, Cheng JD*: FAP-overexpressing fibroblasts produce an extracellular matrix that enhances invasive velocity and directionality of pancreatic cancer cells, BMC Cancer, 11:245. 2011. PMCID: PMC3141768 (*co-corresponding author)

Gupta V, Bassi DE, Simons JD, Al-Saleem T, Devarajan K, Uzzo RG, Cukierman E: Elevated expression of stromal palladin predicts poor clinical outcome in renal cell carcinoma, PLoS ONE 6(6):e21494. 2011. PMCID: PMC3125241

Goetz JG, Minguet S, Navarro-Lerida I, R JJL, Samaniego R, Calvo E, Tello M, Osteso-Ibanez T, Pellinen T, Echarri A, Cerezo A, Klein-Szanto AJ, Garcia R, Keely PJ, Sánchez-Mateos P, Cukierman E, Del Pozo MA: Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis, Cell 146(1):148-63. July 8, 2011. PMCID: PMC3244213

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Håkanson, M; Kobel, S; Lutolf, MP; Textor, M; Cukierman, E*; Charnley, M*: Controlled breast cancer microarrays for the deconvolution of cellular multilayering and density effects upon drug responses, PLoS ONE, 7(6):e40141. 2012 PMCID: PMC3387021 (*co-corresponding author)

Wong, GS, Lee, JS, Park, YY, Klein-Szanto, AJ, Waldron, TJ, Cukierman, E., Herlyn, M, Gimotty, P, Nakagawa, H, and Rustgi AK: Periostin cooperates with mutant p53 to mediate invasion through the induction of STAT1 signaling in the esophageal tumor microenvironment. Oncogenesis, 5(2):e59. 2013. PMCID: PMC3759121

Bassi DE., Cenna J., Zhang J., Cukierman E. and Klein-Szanto A.J: Enhanced aggressiveness of benzopyrene-induced squamous carcinomas in transgenic mice overexpressing the proprotein convertase PACE4 (PCSK6). Molecular Carcinogenesis doi: 10.1002/mc.22183. 2014 PMCID: PMC4240754

Chang YC, Zhang H, Franco-Barraza J, Brennan ML, Patel T, Cukierman E, Wu J. Structural and Mechanistic Insights into the Recruitment of Talin by RIAM in Integrin Signaling. Structure. 22(12):1810-20. doi: 10.1016/j.str.2014.09.020. 2014 PMCID:PMC4255149

Cao X., Lin Y., Driscoll T.P., Franco-Barraza J., Cukierman E., Mauck R.L. and Shenoy V.B. A chemo-mechanical model for extracellular matrix and nuclear rigidity regulated size of focal adhesion plaques. J Biophy. 109(9):1807-17. 10.1016/j.bpj.2015.08.048. 2015 PMCID: PMC4643201.

Roy I, Boyle KA, Vonderhaar EP, Zimmerman NP, Gorse E, Mackinnon AC, Hwang R, Franco-Barraza J, Cukierman E, Tsai S, Evans DB, Dwinell MB: Cancer cell chemokines direct chemotaxis of activated stellate cells in pancreatic ductal adenocarcinoma. Lab Invest. 97(3):302-317. DOI:10.1038/labinvest.2016.146. 2017 PMCID: PMC5334280

Franco-Barraza, J., Francescone R., Luong, T., Shah, N., Madhani, R., Cukierman, G., Dulaimi, E., Devarajan, K., Egleston, B.L., Nicolas, E., Alpaugh, K.R., Malik, R., Uzzo, R.G., Hoffman, J., Golemis E.A. and Cukierman, E. Matrix-regulated integrin αvβ5 maintains α5β1-dependent desmoplastic traits prognostic of neoplastic recurrence. eLife 6:e20600. 2017. PMCID: PMC5283834.

Fragoso MF, Romualdo GR, Vanderveer LA, Franco-Barraza J, Cukierman E, Clapper ML, Carvalho RF, Barbisan LF: Lyophilized açaí pulp (Euterpe oleracea Mart) attenuates colitis-associated colon carcinogenesis while its main anthocyanin has the potential to affect the motility of colon cancer cells. Food and Chemical Toxicology 2018, 121:237-45.

Shafi, A.A., Schiewer, M.J., de Leeuw, R., Dylgjeri, E., McCue, P.A., Shah, N., Gomella, L.G., Lallas, C.D., Trabulsi, E.J., Centenera, M.M., Hickey, T.E., Butler, L.M., Raj, G.V., Tilley, W.D., Cukierman, E., Knudsen, K.E. Patient-derived Models Reveal Impact of the Tumor Microenvironment on Therapeutic Response. European Urology Oncology 2018 (in press).

Publications under preparation, preprints and/or submitted Malik, R., Luong, T., Cao, X., Han, B., Shah, N. Han, L., Shenoy, V.B. Lelkes, P.I., and

Cukierman, E. Rigidity controls human desmoplastic matrix anisotropy to enable pancreatic cancer invasion via extracellular signal-regulated kinase 2. arXiv Preprint submitted in May 2018 https://arxiv.org/abs/1805.02760

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Francescone, R. III., Vendramini-Costa Barbosa, D., Franco-Barraza, J., Wagner, J., Muir, A., Gabitova, L., Pazina, T., Luong, T., Shah, N., Rollins, D., Gupta, S., Thapa, R., Restifo, D., Lau, A., Zhou, Y., Cai, K.Q., Hensley, H., Nicolas, E., Kruger, W., Devarajan, K., Balachandran, S., El-Deiry, W.S., Vander Heiden, M., Campbell, K.S., Astsaturov, I and Cukierman E. NetrinG1/NGL-1 Axis promotes pancreatic tumorigenesis through cancer associated fibroblast derived nutritional supply and immunosuppression. bioRxiv Preprint submitted in June 2018. 330209; doi: https://doi.org/10.1101/330209

EDITORIALS, SOLICITED REVIEWS & TECHNICAL ARTICLES: Editorials: Cukierman, E. Tumour development due to stroma permissiveness. J. Biosci 30:551-552, 2005. Hutmacher DW & Cukierman E. Engineering of tumor microenvironments. Adv Drug Deliv Rev.

15;79-80:1-2. doi: 10.1016/j.addr.2014.11.001. Epub 2014 Nov 8. PMID: 25446131 Reviews: Baum, B.J., Wang, S., Cukierman, E., Delporte, C., Kagami, H., Marmary, Y., Fox, P.C., Mooney,

D.J., Yamada, K. M. Re-engineering the functions of a terminally differentiated epithelial cell in vivo. Bioartificial Organs II. (Annals. N.Y. Acad. Sci.) 875:294-300, 1999.

Cukierman, E., Pankov, R., Yamada, K.M. Cell interactions with three-dimensional matrices. Curr. Opin.Cell Biol. 14:633-640, 2002. (Cover Review, the cover image was requested and is featured in the new edition of Cancer Biology by Robert A. Weinberg).

Yamada, K.M., Pankov, R., Cukierman, E. Dimensions and dynamics in integrin function. Brazilian J. Med. Biol. Res. 36: 959-966, 2003.

Cukierman, E. A visual-quantitative analysis of fibroblastic stromagenesis in breast cancer progression. J. Mammary Gland Biol. Neoplasia 9:311-324, 2004.

Beacham, D.A., Cukierman, E. Stromagenesis: the changing face of fibroblastic microenvironments during tumor progression. Semin. Cancer Biol. 15:329-341, 2005.

Yamada, K. M., Cukierman, E. Modeling tissue morphogenesis and cancer in 3D. Cell. 130:601-610, 2007. (Accepted prior to April 2007)

Cukierman, E., Khan, D. R. The benefits and challenges associated with the use of drug delivery systems in cancer therapy. Biochem. Pharm 1;80(5):762-770, 2010. PMCID: PMC2897922.

Cukierman E, Bassi DE. Physico-mechanical aspects of extracellular matrix influences on tumorigenic behaviors. Semin. Can Biol. 20:139-145. 2010. PMCID: PMC2941524.

Cukierman E and Bassi DE. The mesenchymal tumor microenvironment: a drug resistant niche. Cell Adhesion & Migration, 6(3):285-296, 2012. PMCID: PMC3427243.

Håkanson, M., Cukierman, E*., and Charnley, M*. Miniaturized pre-clinical cancer models as research and diagnostic tools. Advanced Drug Delivery Reviews, 69-70:52-66. 2014. PMCID: PMC4019677 (*co-corresponding author)

Rybinski, B., Franco-Barraza, J., and Cukierman, E. The wound healing, chronic fibrosis and cancer progression triad, Physiological Genomics, 46(7):223-244, 2014. PMCID: PMC4035661

Malik, R., Lelkes, P.I., Cukierman, E. Biomechanical and biochemical remodeling of stromal extracellular matrix in cancer. Trends in Biotech, 33(4):230-236, 2015. PMCID: PMC4380578

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Alexander, J., and Cukierman, E. Stromal dynamic reciprocity in cancer: Intricacies of fibroblastic-ECM interactions. Curr. Opin.Cell Biol. 20(42):80-93 2016 PMID: 27214794 PMCID: PMC5064819

Book Chapters: Huber, I., Rotman, M., Pick, E., Makler, V., Rothem, L., Cukierman, E., Cassel, D. Expression,

purification, and properties of ADP-ribosylation factor (ARF) GTPase activating protein-1. In: Regulation and Effectors of Small GTPases, Part E: GTPases Involved in Vesicular Traffic. (Balch, W.E., Der, Channing, J., Hall, A. eds.). vol. 329, pp. 307-316, Methods Enzymol. Academic Press, San Diego, CA, 2001.

Huber, I., Cukierman, E., Rotman, M., Cassel, D. ARF GTPase-activating protein 1. In: GTPase Protocols: The Ras Superfamily (Manser, E., Leung, T. eds.) vol. 189, pp. 199-206. Methods Mol. Biol. Humana Press, Totowa, NJ, 2002.

Cukierman, E. Preparation of extracellular matrices produced by cultured fibroblasts. In: Current Protocols in Cell Biology (Bonifacino, J.S., Dasso, M., Lippincott-Schwartz, J., Harford, J.B., Yamada, K.M., eds.), pp. 10.9.1-10.9.14. John K. Wiley & Sons, New York, NY, 2002.

Cukierman, E. Cell migration analyses within fibroblast-derived 3D matrices. In: Cell Migration in Development. (Guan, J., ed.), vol. 294, pp. 79-93. Methods Mol. Biol. Humana Press, Totowa, NJ, 2004.

Beacham, D.A., Amatangelo, M.D., Cukierman, E. Preparation of extracellular matrices produced by cultured and primary fibroblasts. (Bonifacino, J.S., Dasso, M., Lippincott-Schwartz, J., Harford, J.B., Yamada, K.M., eds.) pp. 10.9.1-10.9.21. John K. Wiley & Sons, New York, NY, 2007.

Cukierman, E. Three-dimensional tumor-associated stromal system; preparation of extracellular matrices produced by immortalized and primary fibroblasts. Publication for the 2007 Summer School on Nanobiology of the Center for Functional Nanostructures. In press. Bad Herrenalb Germany, August, 19th – 23rd 2008

Cukierman, E. Stromagenesis. In: Encyclopedia of Cancer. (M. Schwab, ed.), pp 2843-2845. Springer, Heidelberg/Germany, 2009.

Castelló-Cros R, Cukierman E: Stromagenesis during tumorigenesis: characterization of tumor-associated fibroblasts and stroma-derived 3D matrices. In: Methods Mol Biol / Extracellular Matrix Protocols. Edited by Even-Ram S, Artym V, vol. 522, 2nd edn. Totowa, NJ,: Humana Press; 2009: 1-31 (275-305).

Valdivia-Silva, J.; Franco-Barraza, J.; Cukierman, E., Garcia-Zepeda, E., Novel Insights into the Role of Inflammation in Promoting Breast Cancer Development. In Breast Cancer - Focusing Tumor Microenvironment, Stem cells and Metastasis, Gunduz, M.; Gunduz, E., Eds. InTech: 2011; Vol. Book 4, pp 129-164.

Available from: http://www.intechopen.com/articles/show/title/novel-insights-into-the-role-of-inflammation-in-promoting-breast-cancer-development

Campbell, C.B., Cukierman, E., and Artym, V.V. 3D extracellular matrix from sectioned human tissues. Curr Protoc Cell Biol, (Bonifacino, J.S., Dasso, M., Lippincott-Schwartz, J., Harford, J.B., Yamada, K.M., eds.) 3;62:19.16.1-19.16.20. John K. Wiley & Sons, New York, NY, 2014. PMCID:PMC4004966

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Franco-Barraza, J., Beacham, D.A., Amatangelo, M.D., and Cukierman, E. Preparation of extracellular matrices produced by cultured and primary fibroblasts. Curr Protoc Cell Biol. 1(71):10.9.1-10.9.34 2016. PMCID: PMC5058441

Cukierman E. Stromagenesis (desmoplasia). Encyclopedia of Cancer. Edited by Schwab M. Berlin, Heidelberg: Springer Berlin Heidelberg, 2017. pp. 4376-9.

INVITED LECTURES: NHLBI, NIH. Branch Seminar Series. Sponsored by Dr. Warren Leonard, 1996. Bethesda, MD,

USA. NIDCR, NIH. Branch Seminar Series. Sponsored by Dr. Hynda Kleinman, 1996. Bethesda, MD,

USA. Lombardi Cancer Center, Georgetown University Medical Center. Seminar series. Sponsored by

Dr. Susette Mueller, 2001. Washington, DC, USA. 2nd International Conference on Tumor Microenvironment Progression, Therapy & Prevention.

Podium Speaker. Sponsored By Dr. Israel Vlodavsky. Tel-Aviv, 2001 (canceled). NIDCR, NIH. Branch Seminar Series. Sponsored by Dr. Silvio Gutkind, 2001. Bethesda, MD,

USA. Fox Chase Cancer Center. Recruiting Committee Seminar Series. Sponsored by Dr. Jonathan

Chernoff, January 2002. Philadelphia, PA, USA. Technion-Israel Institute of Technology, Department of Biology. Recruiting Committee Seminar

Series. Sponsored by Dr. Rami Sherf, February, 2002. Haifa, Israel. Weizmann Institute of Science, Department of Molecular Cell Biology. Recruiting Committee

Seminar Series. Sponsored by Prof. B. Geiger, February 2002. Rehovot, Israel. Technion-Israel Institute of Technology, School of Medicine. Recruiting Committee Seminar

Series. Sponsored by Dr. Israel Vlodavsky, February 2002. Haifa Israel. The Wistar Institute. Laboratory Seminar series. Sponsored by Professor Meenhard Herlyn, July

21, 2003. Philadelphia, PA, USA. Johns Hopkins University, Department of Biomedical Engineering. BME Seminar Series.

Sponsored by Dr. Jennifer Elisseeff, November 14, 2003. Baltimore, MD, USA. University of Pennsylvania, Department of Pharmacology. Seminar Series. Sponsored by Dr.

David Boettiger, January 24, 2005. Philadelphia, PA, USA Keystone Meeting “The Role of Microenvironment in Tumor Induction and Progression” Podium

Speaker. Sponsored by Dr. Mina Bissell. February 5-10, 2005. Banff, Alberta, Canada. NIDCR, NIH. CDBRB, Branch Seminar Series. Sponsored by Dr. Hynda Kleinman, December 8,

2005. Bethesda, MD, USA. Tufts University, Department of Anatomy & Cell Biology. Department Seminar Series. Sponsored

by Dr. Carlos Sonnenschein, March 8, 2006. Boston, MA, USA. The Wistar Institute, Tumor Microenvironment Interest Group Seminar series. Sponsored by

Professors Ellen Pure and Meenhard Herlyn, August 7, 2006. Philadelphia, PA, USA. University of Pennsylvania, Gastroenterology Division. Seminar series. Sponsored by Dr. Jon

Lynch, October 26, 2006. Philadelphia, PA, USA.

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University of Pennsylvania School of Medicine. Muscle Institute’s Seminar Series. Sponsored by Prof. Dennis Discher, December 4, 2006. Philadelphia, PA, USA.

Ben Gurion University, Shraga Segal Memorial Conference. Podium Speaker. Sponsored by Dr. Ron Apte, February 6, 2007. Ber Sheva Israel,

4th International Conference on Tumor Microenvironment: Progression Therapy and Prevention. Podium Speaker. Sponsored by Professor Isaac Witz, March 6-10, 2007 Florence, Italy.

University of California, San Francisco. Comprehensive Cancer Center/Pancreatic Cancer Research Group Seminar Series. Sponsored by Drs. Martin McMahon and Margaret Tempero. May 07, 2007. California, SF, USA.

Universität Karlsruhe, Center for Functional Nanostructures. Nanobiology Summer School. Selected Speaker. Sponsored by Professor Martin Bastmeyer, August 19-23, 2007. Bad Herrenalb, Germany.

Georgetown University School of Medicine. Tumor Biology Training Program’s Visiting Professorship Seminar Series. Sponsored by Drs. Robert Clarke and Claudine Isaacs. October 26, 2007. Washington DC, USA.

New Jersey Medical School. Department of Pathology and Laboratory Medicine, Seminar Series Sponsored by Dr. Marion Cohen. November 29, 2007. Newark, NJ, USA.

Drexel University, Department of Biochemistry Seminar Series. Sponsored by Dr. Mauricio Reginato. December 10, 2007. Philadelphia PA, USA.

Gordon Conference “Signal Transduction by Engineered Extracellular Matrices.” Podium Speaker. Sponsored by Drs. Molly S. Shoichet and Christopher Chen, July 2008. Bates College in Lewiston, Maine, USA.

Ovarian Cancer Research Fund Meeting. Podium Speaker, July 19, 2008. Chicago, IL, USA NIDCR, NIH. Oral and Pharyngeal Cancer Branch Seminar series. Sponsored by Dr. Silvio

Gutkind. September 24, 2008. Bethesda MD, USA Ohio State University Medical Center. Department of Pathology and Tumor Microenvironment

Program Seminar series. Sponsored by Drs. Steve Qualman and Michael Ostrowski. October 1, 2008. Columbus, OH USA.

Keystone Meeting “Extrinsic Control of Tumor Genesis and Progression” Podium Speaker. Sponsored by Drs. Thea D. Tlsty and Mary J.C. Hendrix. March 15-20, 2009. Vancouver, British Columbia.

Wound Healing Society National Meeting 2009. "Micro-environment: Wound vs. Cancer." Plenary Session, Podium Speaker. Invited by Dr. Tai-Lan Tuan, April 26-29, 2009. Dallas, Texas, USA.

Abbott Laboratories. Institute’s Seminar Series. Sponsored by Vivek C. Abraham, Ph.D. August 2009. Abbott Park , IL.

Swiss Federal Institute of Technology (ETH Zurich). BioInterface Group, Laboratory for Surface Science and Technology Department of Materials. Departmental Seminar series. Sponsored by Dr. Marcus Textor, Ph.D. March 22 and 23, 2010. Zurich, Switzerland.

Centocor Research & Development, Inc. Ortho Biotech Oncology’s Seminar series. Sponsored by Dr. Deborah Marshall Ph.D. June 23, 2010. King of Prussia Radnor, PA.

Gordon Conference “Signal Transduction by Engineered Extracellular Matrices.” Session Chair. Sponsored by Drs. Christopher Chen and Karen Hirschi, June-July 2010. University of New England in Biddeford, Maine, USA.

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University of Massachusetts Medical School, Cancer Biology Program Seminar Series. Sponsored by Dr. Lucia Languino. December 7, 2010. Worcester, MA USA. Postponed by the organizers due to logistic problems.

Temple University, Department of Biochemistry together with the Fels Institute for Cancer Research joint Seminar Series. Sponsored by Dr. Ana Gamero, December 6, 2010. Philadelphia, PA.

Northwestern/Robert H. Lurie Comprehensive Cancer. Tumor Cell Biology Seminar Series. Sponsored by Dr. Jennifer Koblinsky, February 17, 2011. Chicago Il.

Thomas Jefferson University, Jefferson Medical College, Department of Surgery Seminar Series. Sponsored by Dr. Susan Lanza-Jacoby, March 3. 2011. Philadelphia PA.

The 6th International Conference on Microtechnologies in Medicine and Biology (MMB 2011). “Keynote Lecture.” Sponsored by Dr. Shuichi Takayama, May 4-6, 2011. Lucerne, Switzerland.

1st Fox Chase Cancer Center Breast Cancer Scientific Retreat. Invited Speaker. Sponsored by Drs. Massimo Cristofanilli, MD & Lori Goldstein, MD, October 29, 2011.

Thomas Jefferson University, Jefferson Medical College, Department of Cancer biology at the Kimmel Cancer Center Seminar Series. Sponsored by Dr. Lucia Languino, November 17. 2011. Philadelphia PA.

Keystone Symposium in Fibrosis at Big Sky Montana. Selected Short Talk. April 1, 2012. Secant Medical LLC. Special seminar invitation sponsored by Peter Gabriele (Director,

Emerging Technology). June 4, 2012. Perkasie, PA. Institute of Bioengineering and Nanotechnology, The Nanos, Singapore. Institute of

Bioengineering and Nanotechnology Seminar Series. Sponsored by Dr. Andrew C.A. Wan, Summer 2012 (postponed for personal reasons), The Nanos, Singapore.

Temple University – Fox Chase Cancer Center/Cancer Biology Scientific Retreat. Sponsored by Dr. Jonathan Chernoff (FCCC Chief Scientific Officer). November 9, 2012. Philadelphia, PA.

Esperanza College; invited talk delivered in Spanish with the goal to share career experiences with students in the health sciences. November 21, 2012 (postponed and conducted on February 18, 2013). Philadelphia, PA.

University of Delaware; Department of Biological Sciences Seminar Series. Sponsored by Drs. Erica Selva and Carlton R. Cooper, November 28, 2012. Newark, DE.

Louisiana State University; Department of Biochemistry and Molecular Biology, Stanley S. Scott Cancer Center Seminar Series. Sponsored by Dr. Suresh K. Alahari, November 30, 2012. New Orleans, LA.

Springside Chestnut Hill Academy; Science Department. Invited talk to share career experiences with students in AP Biology. Sponsored by Scott Stein, March 8, 2013. Philadelphia, PA.

University of Oklahoma Health Science Center, Department of Physiology 2013-2014 Seminar Series. Sponsored by Dr. Xin Zhang, September 12, 2013. Oklahoma City, OK.

University of Kentucky, Markey Cancer Center Seminar series. Sponsored by Drs. Ren Xu and Mark Evers, October 23, 2013. Lexington, KY.

University of Pennsylvania, Department of Animal Biology/School of Veterinary Medicine, Mari Lowe Center for Comparative Oncology Seminar Series. Sponsored by Dr. Serge Fuchs, October 31, 2013. Philadelphia, PA.

Materials Research Society 2013 Fall Meeting. Invited Speaker at the ‘Multi-scale Materials in the Study and Treatment of Cancer’ Symposia. December 1-6, 2013. Boston, MA.

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Rensselaer Polytechnic Institute, Department of Biological Sciences Seminar Series. Sponsored by Drs. Susan P. Gilbert & Lee Ligon, September 16, 2014. Troy, NY.

Temple University School of Medicine. Fels Institute for Cancer Research & Molecular Biology Seminar Series. Sponsored by Dr. Raza Zaidi. September 23, 2014. Philadelphia PA.

New Frontiers in Cancer research at the University of Texas MD Anderson Cancer Center. Invited Speaker. Sponsored by Dr. Keping Xie, September 25, 2014. Huston, TX.

Temple University Department of Bioengineering Seminar series. Sponsored by Drs. Won Suh & Peter Lelkes. October 10, 2014. Philadelphia, PA.

Brown University, School of Engineering Seminar Series. Sponsored by Dr. Ian Wong, October 16, 2014. Providence, RI.

XLIV National Meeting of Clinical Pathology. Invited lecture at the New Discoveries in Cancer Biology and Tumor Microenvironment symposium. Sponsored by the Mexican Federation of Clinical Pathology and the Oaxacan Society of Clinical Pathology (invited by Dr. R. Franco Santillán). October 29 to November 1, 2014, Mexico City.

Cambridge Healthtech Institute’s Second Annual “Screening and Functional Analysis of 3D Models” meeting– invited seminar. This meeting is part of the Second Annual FAST: Functional Analysis & Screening Technologies Congress. Sponsored by Dr. Julia Boguslavsky. November 18-19, 2014, Boston, MA.

Mayo Clinic Cancer Center Seminar Series. Sponsored by Dr. Derek Radisky, December 16, 2014. Jacksonville, FL.

Temple Univ. School of Medicine. Center for Inflammation, Translational and Clinical Lung Research Seminar Series. Sponsored by Dr. Thomas J. Rogers, March 27, 2015. Philadelphia, PA.

University of Pennsylvania’s 1st Fibrosis Symposium. Scientific Talk Invited by Drs. Ellen Puré, Rebecca Wells and Katalin Susztak. April 17, 2015. Philadelphia, PA.

West Virginia University. Biochemistry and MBR Cancer Center Seminar Series. Sponsored by Dr. Elena N. Pugacheva, September 2, 2015. Morgantown, WV.

Wistar Institute. Molecular and Cellular Oncogenesis Seminar Series. Sponsored by Drs. Maureen Murphy and Ashani Weeraratna. September 8, 2015. Philadelphia, PA.

Roswell Park Cancer Institute. Department of Cancer Genetics Seminar Series. Sponsored by Dr. Jianmin Zhang, September 15, 2015. Buffalo, NY.

University of Pennsylvania Perelman School of Medicine Division of Gastroenterology and NIDDK P30 Center for Molecular Studies in Digestive and Liver Diseases’ Seminar Series. Sponsored By Dr. Anil Rustgi. October 1, 2015. Philadelphia PA.

Fox Chase Cancer Center Symposium, “Adjuvant therapy and borderline resectable neoadjuvant” honoring Dr. John P. Hoffman. Invited Speaker. October 9, 2015. Philadelphia, PA.

VII International Conference on Tumor Microenvironment Progression, Therapy & Prevention by the International. Cancer Microenvironment Society. Invited Seminar/Talk. Sponsored by the Cancer Microenvironment Society (Invited by the Society’s President Dr. Isaac Witz). October 11-15, 2015. Tel-Aviv, Israel.

The Hebrew University of Jerusalem, Institute for Drug Research Faculty of Medicine Seminar Series. Sponsored by Dr. Philip Lazarovici. October 18, 2015. Jerusalem Israel.

Technion Institute of Technology, Biology Department Seminar Series. Sponsored by Dr. Dan Cassel. October 19, 2015. Haifa Israel

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Iowa State University. Cell Biology Seminar Series. Sponsored by Dr. Ian C Schneider. Invited Speaker. November 12, 2015. Ames, IA.

University of Pennsylvania, Physical Sciences of Oncology Center Seminar Series. Sponsored by Dr. V Shenoy. February 8, 2016. Philadelphia PA.

Medical College of Wisconsin Cancer Center, Pancreas Cancer Research Center Seminar Series. Sponsored by Dr. Ming You. March 17, 2016. Milwaukee WI.

Distinguished Achievement Award in Cancer Research and Clinical Management. Plenary Session Cancer Forum Award with emphasis in Pancreatic Cancer at The Shanghai East Cancer 2016 Symposium of The Chinese Society of Clinical Oncology (Chinese version of ASCO). Awarded by Yong Gao from Shanghai’s East Hospital & Keping Xie from MD Anderson. May 24, 2016. Shanghai China.

Together Facing Pancreatic Cancer by Pancreatic Cancer Action Network. Educational Speaker and Panelist. June 15, 2016. Fox Chase Cancer Center. Philadelphia, PA.

Gordon Research Conference/Signaling by Adhesion Receptors. Invited Speaker. Sponsored by Drs. Alpha Yap and Christopher Chen. Bates College, Lewiston, ME. June 19-24, 2016.

Penn State Cancer Institute, Department of Pharmacology Seminar Series. Sponsored by Dr. Nadine Hempel. September 15 2016. Hershey, PA.

Wayne State University, Department of Biological Sciences Seminar Series. Sponsored by Dr. Karen Beningo. October 17 2016. Detroit, MI.

High Content Analysis, Cambridge Healthtech Institutes 14th Annual Meeting, Invited Speaker and Short Course Modulator at Screening and Functional Analysis of 3D Models Session. October 31st to November 2nd 2016. Boston, MA.

University of Toledo, Department of Biological Sciences Seminar Series. Sponsored by Dr. Rafael Garcia-Mata. November 4, 2016. Toledo, OH.

Sidney Kimmel Cancer Center/TJU, Philadelphia Area Prostate Cancer Working Group Seminar Series. Sponsored by Dr. Alessandro Fatatis. February 8, 2017. Philadelphia, PA.

Gordon Research Conference/ Tissue Repair & Regeneration. Invited Speaker by Dr. Boris Hinz. Colby-Sawyer College, New London, NH. June 4-9, 2017.

Annual Retreat of the Department of Regenerative & Cancer Cell Biology. Keynote Speaker Selected by the Student Committee. Albany Medical College at Albany, NY. July 31, 2017.

Cold Spring Harbor Laboratory’s Biology of Cancer: Microenvironment & Metastasis Meeting. Invited Speaker; Tumor Immunology Session. New Jersey, NY. October 10-14, 2017.

Drexel Symposium on Molecular Medicine and Infectious Disease. Invited Speaker. Hahnemann Hospital, Drexel University. Philadelphia, PA. November 15, 2017.

Tel Aviv University, Neurobiology Department Seminar Series. Invited Speaker; Hosted by Professor Reuven Stein. Tel Aviv, Israel. December 19, 2017.

University of California Davis, Comprehensive Cancer Center Seminar Series. Sponsored by Dr. Su Hao Lo. February 7, 2018. Sacramento, CA.

The Icahn School of Medicine 2018 Translational Oncology Seminar Series. Invited by Dr. Javier Bravo-Cordero. Division of Hem/Onc Mt. Sinai Hospital. New York City, NY March 6, 2018.

Gordon Conference “Signaling By Adhesion Receptors.” Evolving Concepts in Adhesion Signaling and Cancer Progression Session Chair/Discussion Leader. Sponsored by Drs. Christopher Chen and Anne Miller, June 24-29, 2018. University of New England in Biddeford, Maine, USA.

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17th International Biennial Congress of the Metastasis Research Society. Invited Speaker by Session/Meeting Chairs Drs. Weeraratna, Kang & Yu. Princeton Univ., NJ. August 1-5, 2018.

University of Bergen Seminar Series. Invited by Donald Gullberg. Bergen Norway. August 30, 2018

2nd CellulART Meeting at the Toledo Art Museum. Featured Scientist/Artist Talk. Invitation by Dr. Raphael Garcia Mata. September 14, 2018. Toledo, OH.

Biology Department Symposium in Honor of Professor Dan Cassel’s Retirement (PhD Mentor). Invited by organizer, Professor Benjamin Podbilewicz. Technion Institute of Technology. October 15, 2018. Haifa, Israel

Ben Gurion University Wound Healing Hub Workshop Speaker. Invited by Drs. Eli Lewis & Ronen Shuster. October 18 & 19, 2018, Ber Sheva, Israel.

Distinguished Scientist Seminar Series, Department of Molecular and Cellular Oncology (2018-2019) at The University of Texas M.D. Anderson Cancer Center. Invited by Drs. Mien-Chie Hung and Dihua Yu. November 7, 2018. Huston, TX.

Indiana University 2018-2019 Cancer Biology Seminar Series. Medical Science Program, Indiana University Medical School. Invited by Anirban K Mitra. January 14, 2019. Bloomington, IN.

Special Banbury Center of Cold Spring Harbor Laboratory Meeting on Cancer Fibroblasts and Therapies. Invited by Dr. David Tuveson. March 10-13, 2019. Lloyd Harbor, Ney York.

Speaker at the Circulating Biomarkers/Liquid Biopsies Meeting. Invited by Drs. Lucia Languino and Enal Razvi. March 27-29, 2019, San Diego, CA.

GRANT SUPPORT: Ongoing Research Support FCCC Translational Clinical Protocol Development (MPI: Cukierman) 04/15/2018 – 04/14/2019 Utilizing pulsed Low-dose-rate radiation to prevent radiation-induced stromal activation; a neoadjuvant pancreatic adenocarcinoma phase I trial Role: Co-Principal Investigator (with Joshua Meyer MD)

T32 CA009035 (PI: Chernoff) 8/01/2017 - 7/31/2019 NIH Role: Cukierman Mentor for Gardiner Training Program in Cancer Research The overall goal of this program is to prepare postdoctoral scientists for independent careers in basic and/or translational research focused on cancer.

Greenberg Fund for Pancreatic Cancer Research (PI: Cukierman) 11/15/2014 – 11/14/2020 A multi-PI study to promote Pancreatic Cancer collaborative research at FCCC. Role: co-Principal Investigator

Ongoing research/contracts throughout 2018-2019 with: -Esai Inc -Geistlich Pharma -Amal Therapeutics -Oncoceutics

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Pending Research Support R21 CA223539-01A1 (PI Cukierman) expected for 2019-2020 NIH/NCI (obtained 8% on A0, A1 submitted) Investigating how desmoplasia gets on pancreatic cancer’s nerves. RFA CA-93-003. Role: Principal Investigator

R01 CA232256 (MPI Cukierman; A0 scored in 14%) expected for 2019-2023 NIH/NCI (to be reviewed in Oct 2018) An integrated approach to melanoma metastasis and therapy resistance: effects of age-related changes in the ECM and the biomechanics of the skin. Role: Co-Principal Investigator

R21 CA231252-01A1 (MPI Cukierman) expected for 2019-2020 NIH/NCI Provocative Questions (A1 submitted) Oncogenic Synapses: cell-cell contacts enabling trogocytic-based metabolic interactions between pancreatic cancer and fibroblastic stromal cells. FOA: [CA18-020] Role: Co-Principal Investigator

R01 CA232582 (PI Cukierman) expected for 2019-2023 NIH/NCI (to be reviewed in Spring 2019) A desmoplastic integrin switch that supports pancreatic cancer. Role: Principal Investigator

R01 CA186564 (PI Cukierman) expected for 2019-2023 NIH/NCI (to be re-submitted in Spring 2019) Preclinical study of ITPKA roles in renal cell carcinoma Role: Principal Investigator

In Preparation U01 (MPI Cukierman) expected for 2020-2024 NIH/NCI (in preparation for submission in Fall 2019) This is a multidisciplinary collaboration with PIs from Drexel, UNC, UPenn and FCCC. Role: Co-Principal Investigator

Completed Research Support RO1 CA113451 (PI: Cukierman) 07/01/2006 – 03/31/2018 NIH/NCI 3D-adhesion Stromagenesis in Cancer Permissiveness The major goals of this project are: 1) Study structural and biochemical components of the tumor-associated ECM; 2) establish the role for palladin in the matrix induced stromal phenotypic switch; and 3) Assess the clinical importance of stromal activation in human pancreatic cancer and renal cell carcinoma. Role: Principal Investigator

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R01 CA113451 Supp 2 (PI: Cukierman) 4/1/2015 - 3/31/2018 NCI/NIH 3D-adhesion Stromagenesis in Cancer Permissiveness Research supplement to promote diversity; associated to the parent grant.

R01 CA113451 Supp 1 (PI: Cukierman) 8/1/2014 - 3/31/2018 NCI/NIH 3D-Adhesion Stromagenesis in Cancer Permissiveness Administrative Supplement to support ongoing efforts aligned with the parent grant.

DOD W81XH-15-1-0170 (PI: Cukierman) 09/15/2015-09/15/2017 Idea Award with Special Focus in Military Personnel-Affected Pancreatic Cancer. Pancreatic Cancer’s Desmoplasia: The Possible Bridge Impending Nerve Infiltration and Neoplastic Escape. Role: Principal Investigator PanCan (PI: Astsaturov) 07/1/2015 – 06/30/2017 Drug conjugates as a “Trojan horse” approach to treat pancreatic cancer Role: co-Investigator

Funds in Support of Esophageal Cancer Research (PI: Cukierman) 05/01/2106 – 04/30/2017

T32 CA009035 (PI: Chernoff) 8/18/2014 - 8/17/2017 NIH Role: Cukierman Mentor for Francescone

Nodal Temple-FCCC multi PI Grant (PIs: Lelkes & Cukierman) 08/01/2013 – 07/31/2015 Temple/FCCC. Mechanotransduction and the regulation of human pancreatic cancer progression Role: Principal Investigator

Kidney Keystone - Pilot Project (PI: Cukierman) 07/01/2009 – 06/30/2014 A study of renal primary and secondary tumor-associated stroma The major goal of this project is to establish and characterize a human primary and secondary 3D renal-stroma system. Role: Principal Investigator

Bucks County Board of Directors – 18 month Project (PI: O’reilly) 01/01/2013 – 06/30/2014 A multi-PI study to address sHh influences in pancreatic tumor-stromal interactions. Role: co-Principal Investigator

Centocor/Janssen (J&J) Contract – 3 year Project (PI: Cukierman) 01/01/2011 – 12/31/2013 Lung Stromagenesis The goal of the study is to better understand lung cancer associated stroma progression Role: Principal Investigator

American Urology Association Foundation (PI: Gupta) 07/01/2011 – 06/01/2012 The role of activated tumor-associated stroma in supporting renal cell carcinoma progression. Role: Mentor

Ovarian Cancer Research Fund Program project (OCRF) (PI: Godwin) 10/01/2005 – 09/30/2011 Therapeutic targeting of the tumor microenvironment in ovarian cancer Role: Co-Principal Investigator

CUKIERMAN, Edna

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Ewing Trust, Pancreatic Cancer (PI: Cukierman) 09/01/2007 – 05/31/2010 Pancreatic stroma progression: microenvironmental-induced tumorigenesis. Role: Principal Investigator

R21 CA109442 NCI (PI: Cukierman) 09/07/2004 – 08/31/2007 Primed Stroma: A Tumor Permissive Microenvironment Role: Principal Investigator

Susan G. Komen Foundation grant (PI: Maffini, Tufts) 05/01/2006 – 04/30/2008 The tumor suppressing effect of pregnancy: can the mammary stroma do the job? Given the new evidence regarding the role of the stroma in suppressing tumor formation in parous rats, we propose to use a new model system that will allow us to study the effect of pregnancy-associated hormones locally in the mammary stroma. Role: Collaborator at FCCC (This project is a subcontract to Tufts University)

W.W. Smith Charitable Trust (PI: Cukierman) 11/01/2005 – 12/31/2008 3D Matrix Cultures for Better Anti-Metastatic Drug Screening Role: Principal Investigator

AACR (PI: Cukierman) 07/01/2006 – 06/30/2007 The Role of Progressive Stroma Signaling in Cancer Permissiveness The major goal of this Career Development Award in Basic Cancer Research was to develop a 3D matrix-based novel high throughput system, which will score tumor cell invasiveness. Role: Principal Investigator

NCI/NIH Ovarian SPORE Pilot Project (PI: Cukierman) 06/01/2006 – 05/31/2007 Ovarian stroma progression; targeting the tumor microenvironment. Aimed to investigate whether previous in vitro observations could be validated through multi-protein labeling tumor specimens from women with ovarian cancer, as well as to test whether matrices derived from human fibroblasts at different stages of tumor progression, could differentially induce ovarian tumorigenesis. Role: Principal Investigator

Ewing Trust, Pancreatic Cancer (PI: Cukierman) 09/01/2005 – 08/31/2006 An in vivo-like 3D system to study stroma permissiveness in pancreatic neoplasia This study was based on effort to develop a human staged pancreatic stromagenic 3D system. Role: Principal Investigator

AACR (PI: Cukierman) 07/01/2004 – 06/30/2006 In Vivo-Like 3D System to Assess Stroma Permissiveness in Tumor Cell Invasion The major goal of this award was to develop a 3D matrix-based novel high throughput system, which will score tumor cell invasiveness and will assess effectiveness of cancer drugs in metastases. Role: Principal Investigator

NCI/NIH Ovarian SPORE Pilot Project (PI: Cukierman) 04/01/2005 – 05/31/2006 The three-dimensional signaling pathway and its implication on the study of ovarian cancer Aimed to utilize ovarian cancer-associated fibroblasts to produce 3D matrices. The matrices were used as a model system to study stromagenic cancer permissiveness in ovarian cancer. Role: Principal Investigator

CUKIERMAN, Edna

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National Pancreas Foundation (PI: Cukierman) 05/01/2004 – 04/30/2005 A Pilot Study to Model the Role of Fibroblasts in the Extracellular Matrix of Patients with Pancreatic Cancer. This pancreas cancer project proposed to utilize fine needle aspirates of pancreatic cancer of patients to establish a human stromagenic 3D system for the study of pancreatic cancer permissiveness. Role: Principal Investigator

Tobacco Formula Research Fund Grant (PI: Leedy) 01/01/2003 – 06/30/2004 Pennsylvania Health Department Interactions among Cells and Natural Surroundings The goal of this project was to understand the structural interactions among cancerous cells and their natural surroundings in order to prevent them from invading other tissue and stop cancer progression at the cellular level. Role: Co-Principal Investigator

PATENTS -Baum, B.J., Yamada, K.M., Cukierman, E., Mooney, D. Patent No. 6,743,626 “Artificial Salivary Gland”, 2004. The present invention generally relates to the field of oral prosthetics and tissue engineering. More specifically, a novel, artificial fluid secreting prosthesis for non-invasive insertion is disclosed. Further, methods of use of the foregoing are provided.

-Cukierman, E. Franco-Barraza, J., Shah, N. (and Humphries, M.J.). WO2018005355A1 “Active alpha5-beta1 integrin as a biomarker for enhancing tumor treatment efficacy.” January 1, 2018 -

- Cukierman, E., Lelkes, P.I., and Malik, R. "Preparation of 3D cell-derived extracellular matrices on polyacrylamide gels for studying cancer-ECM interactions in pancreatic carcinoma." Provisional invention was filed 2/13/2018

-Another provisional (therapeutic) invention is pending and remains confidential.