CTBE Publications: 2010

8/3/2019 CTBE Publications: 2010

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The Brazilian Bioethanol Science and Technology Laboratory (CTBE) publications printed on 2010and indexed by Web of Sciences (www.isiwebofknowledge.com) are listed below in chronologicalorder.Criteria for articles selectionFill (CTBE OR Bioethanol OR Bioetanol) AND Brazil in the Address Search form.

Total of CTBE Journal Articles15.

15. Thermal-induced conformational changes in the product release area drive the enzymaticactivity of xylanases 10B: Crystal structure, conformational stability and functional

characterization of the xylanase 10B from Thermotoga petrophila RKU-1

Author(s): Santos, CR; Meza, AN; Hoffmam, ZB; Silva, JC; Alvarez, TM; Ruller, R; Giesel, GM; Verli,H; Squina, FM; Prade, RA; Murakami, MT | E-mail:[email protected] Source: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Volume: 403 Issue:2 Pages: 214-219 | DOI: 10.1016/j.bbrc.2010.11.010 | Published: DEC 10 2010

Abstract: Endo-xylanases play a key role in the depolymerization of xylan and recently, they haveattracted much attention owing to their potential applications on biofuels and paper industries. In thiswork, we have investigated the molecular basis for the action mode of xylanases 10B at hightemperatures using biochemical, biophysical and crystallographic methods. The crystal structure ofxylanase 10B from hyperthermophilic bacterium Thermotoga petrophila RKU-1 (TpXyl10B) has been

solved in the native state and in complex with xylobiose. The complex crystal structure showed aclassical binding mode shared among other xylanases, which encompasses the -1 and -2 subsites.Interestingly, TpXyl10B displayed a temperaturedependent action mode producing xylobiose andxylotriose at 20 degrees C, and exclusively xylobiose at 90 degrees C as assessed by capillary zoneelectrophoresis. Moreover, circular dichroism spectroscopy suggested a coupling effect oftemperature-induced structural changes with this particular enzymatic behavior. Molecular dynamicssimulations supported the CD analysis suggesting that an open conformational state adopted by thecatalytic loop (Trp297-Lys326) provokes significant modifications in the product release area (+1,+2and +3 subsites), which drives the enzymatic activity to the specific release of xylobiose at hightemperatures. (C) 2010 Elsevier Inc. All rights reserved.Full Text:http://www.sciencedirect.com/science/article/pii/S0006291X10020589

Addresses: [Santos, CR; Meza, AN; Murakami, MT] Ctr Nacl Pesquisa Energia & Mat, Lab NaclBiociencias LNBio, BR-13083970 Campinas, SP, Brazil[Hoffmam, ZB; Silva, JC; Alvarez, TM; Ruller, R; Squina, FM] Ctr Nacl Pesquisa Energia & Mat, LabNacl Ciencia & Tecnol Bioetanol CTBE, BR-13083970 Campinas, SP, Brazil[Giesel, GM; Verli, H] Univ Fed Rio Grande do Sul, Ctr Biotecnol, Porto Alegre, RS, Brazil[Prade, RA] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 USA

14. Involvement of the Aspergillus nidulans protein kinase C with farnesol tolerance is related
http://www.isiwebofknowledge.com/http://www.isiwebofknowledge.com/http://www.isiwebofknowledge.com/mailto:[email protected]:[email protected]:[email protected]://www.sciencedirect.com/science/article/pii/S0006291X10020589http://www.sciencedirect.com/science/article/pii/S0006291X10020589http://www.sciencedirect.com/science/article/pii/S0006291X10020589http://www.sciencedirect.com/science/article/pii/S0006291X10020589mailto:[email protected]://www.isiwebofknowledge.com/


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to the unfolded protein response

Author(s): Colabardini, AC; de Castro, PA; de Gouvea, PF; Savoldi, M; Malavazi, I; Goldman, MHS;Goldman, GH | E-mail:[email protected] Source: MOLECULAR MICROBIOLOGY Volume: 78 Issue: 5 Pages: 1259-1279 | DOI:10.1111/j.1365-2958.2010.07403.x | Published: DEC 2010

Abstract: P>Previously, we demonstrated that the Aspergillus nidulans calC2 mutation in proteinkinase C pkcA was able to confer tolerance to farnesol (FOH), an isoprenoid that has been shown toinhibit proliferation and induce apoptosis. Here, we investigate in more detail the role played by A.nidulans pkcA in FOH tolerance. We demonstrate that pkcA overexpression during FOH exposurecauses increased cell death. FOH is also able to activate several markers of endoplasmic reticulum(ER) stress and the unfolded protein response (UPR). Our results suggest an intense cross-talkbetween PkcA and the UPR during FOH-induced cell death. Furthermore, the overexpression of pkcAincreases both mRNA accumulation and metacaspases activity, and there is a genetic interactionbetween PkcA and the caspase-like protein CasA. Mutant analyses imply that MAP kinases areinvolved in the signal transduction in response to the effects caused by FOH.Full Text:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2010.07403.x/abstract

Addresses: [Colabardini, AC; de Castro, PA; de Gouvea, PF; Savoldi, M; Goldman, GH] Univ SaoPaulo, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo, Brazil[Goldman, MHS] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Sao Paulo, Brazil[Malavazi, I] Univ Fed Sao Carlos, Dept Genet & Evolucao, CCBS, BR-13560 Sao Carlos, SP, Brazil[Goldman, GH] Lab Nacl Ciencia & Tecnol Bioetanol CTBE, BR-13083970 Sao Paulo, Brazil

13. The roles played by Aspergillus nidulans apoptosis-inducing factor (AIF)-like mitochondrialoxidoreductase (AifA) and NADH-ubiquinone oxidoreductases (NdeA-B and NdiA) in farnesolresistance

Author(s): Dinamarco, TM; Pimentel, BDF; Savoldi, M; Malavazi, I; Soriani, FM; Uyemura, SA;

Ludovico, P; Goldman, MHS; Goldman, GH | E-mail:[email protected] Source: FUNGAL GENETICS AND BIOLOGY Volume: 47 Issue: 12 Pages: 1055-1069 | DOI:10.1016/j.fgb.2010.07.006 | Published: DEC 2010

Abstract: Farnesol (FOH) is a nonsterol isoprenold produced by dephosphorylanon of farnesylpyrophosphate a catabolite of the cholesterol biosynthetic pathway These isoprenoids inhibitproliferation and induce apoptosis Here we show that Aspergillus nidulans MA encoding theapoptosis-Inducing factor (AIF)-like mitochondrial oxidoreductase plays a role in the function of themitochondrial Complex I Additionally we demonstrated that ndeA B and ndiA encode external andinternal alternative NADH dehydrogenases respectively that have a function in FOH resistance Whenexposed to FOH the Delta aifA and Delta ndeA strains have increased ROS production while DeltandeB Delta ndeA Delta ndeB and Andul mutant strains showed the same ROS accumulation than in

the absence of FOH We observed several compensatory mechanisms affecting the differentialsurvival of these mutants to FOH (C) 2010 Elsevier Inc All rights reservedFull Text:www.sciencedirect.com/science/article/pii/S1087184510001325

Addresses: [Dinamarco, TM; Pimentel, BDF; Savoldi, M; Soriani, FM; Uyemura, SA; Goldman, GH]Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14040903Sao Paulo, Brazil[Malavazi, I] Univ Fed Sao Carlos, CCBS, Dept Genet & Evolucao, BR-13560 Sao Carlos, SP, Brazil[Ludovico, P] Univ Minho, Sch Hlth Sci, Life & Hlth Sci Res Inst ICVS, Braga, Portugal[Goldman, MHS] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Sao Paulo, Brazil
mailto:[email protected]:[email protected]:[email protected]://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2010.07403.x/abstracthttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2010.07403.x/abstracthttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2010.07403.x/abstractmailto:[email protected]:[email protected]:[email protected]://www.sciencedirect.com/science/article/pii/S1087184510001325http://www.sciencedirect.com/science/article/pii/S1087184510001325http://www.sciencedirect.com/science/article/pii/S1087184510001325http://www.sciencedirect.com/science/article/pii/S1087184510001325mailto:[email protected]://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2010.07403.x/abstractmailto:[email protected]


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[Goldman, GH] Univ Sao Paulo, Lab Nacl Ciencia & Tecnol Bioetanol, Sao Paulo, Brazil

12. Seed Cell Wall Storage Polysaccharides: Models to Understand Cell Wall Biosynthesis andDegradation

Author(s): Buckeridge, MS | E-mail:[email protected]

Source: PLANT PHYSIOLOGY Volume: 154 Issue: 3 Pages: 1017-1023 | DOI:10.1104/pp.110.158642 | Published: NOV 2010

Abstract: Cell wall storage polysaccharides (CWSPs) are found as the principal storage compoundsin seeds of many taxonomically important groups of plants. These groups developed extremelyefficient biochemical mechanisms to disassemble cell walls and use the products of hydrolysis forgrowth. To accumulate these storage polymers, developing seeds also contain relatively high activitiesof noncellulosic polysaccharide synthases and thus are interesting models to seek the discovery ofgenes and enzymes related to polysaccharide biosynthesis. CWSP systems offer opportunities tounderstand phenomena ranging from polysaccharide deposition during seed maturation to the controlof source-sink relationship in developing seedlings. By studying polysaccharide biosynthesis anddegradation and the consequences for cell and physiological behavior, we can use these models to

develop future biotechnological applications.Full Text:http://www.plantphysiol.org/content/154/3/1017.full

Addresses: [Buckeridge, MS] Univ Sao Paulo, Dept Bot, BR-05422970 Sao Paulo, Brazil[Buckeridge, MS] Natl Ctr Res Energy & Mat, Brazilian Bioethanol Sci & Technol Lab, BR-13083970Campinas, SP, Brazil

11. Electric and spectroscopic properties of argon-hydrogen RF microplasma jets atatmospheric pressure

Author(s): Souza-Correa, JA; Oliveira, C; Gomes, MP; Amorim, J | E-mail:

[email protected]: JOURNAL OF PHYSICS D-APPLIED PHYSICS Volume: 43 Issue: 39 Article Number:395203 | DOI: 10.1088/0022-3727/43/39/395203 | Published: OCT 6 2010

Abstract: Microplasma jets of argon-hydrogen (Ar-H(2)) gas mixture were generated by 144.0 MHzradio-frequency (RF) waves at powers of 5W, 10W, 20W and 50W. The experimental setup employedcreates stable microplasmas at atmospheric pressure from 5.0mm up to 20.0mm visual glow lengths.We have determined the rms voltages, the rms electric currents and the power absorptions of thesemicroplasma jets. By making use of optical spectroscopy, the emission spectra of Ar-H(2)microplasma jets were recorded in the range 3060-8200 angstrom, in order to estimate the axialdistribution profiles of electron density, rotational temperature, excitation temperature and hydrogenatomic temperature.

Full Text:http://iopscience.iop.org/0022-3727/43/39/395203/

Addresses: [Souza-Correa, JA; Oliveira, C; Amorim, J] Lab Nacl Ciencia & Tecnol Bioetanol CTBE,BR-13083970 Campinas, SP, Brazil[Souza-Correa, JA; Gomes, MP] ITA, Dept Fis, BR-12228900 Sao Jose Dos Campos, SP, Brazil

10. Possibility of nonexistence of hot and superhot hydrogen atoms in electrical discharges

Author(s): Loureiro, J; Amorim, J | E-mail:[email protected]
mailto:[email protected]:[email protected]:[email protected]://www.plantphysiol.org/content/154/3/1017.fullhttp://www.plantphysiol.org/content/154/3/1017.fullhttp://www.plantphysiol.org/content/154/3/1017.fullmailto:[email protected]:[email protected]://iopscience.iop.org/0022-3727/43/39/395203/http://iopscience.iop.org/0022-3727/43/39/395203/http://iopscience.iop.org/0022-3727/43/39/395203/mailto:[email protected]:[email protected]:[email protected]:[email protected]://iopscience.iop.org/0022-3727/43/39/395203/mailto:[email protected]://www.plantphysiol.org/content/154/3/1017.fullmailto:[email protected]


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Source: PHYSICAL REVIEW E Volume: 82 Issue: 3 Article Number: 035401 | DOI:10.1103/PhysRevE.82.035401| Published: SEP 3 2010

Abstract: Recently, the existence of extremely energetic hydrogen atoms in electrical discharges hasbeen proposed in the literature with large controversy, from the analysis of the anomalous broadeningof hydrogen Balmer lines. In this paper, the velocity distribution of H atoms and the profiles of theemitting atom lines created by the exothermic reaction H(2)(+) + H(2) -> H(3)(+) + H + Delta E are

calculated, as a function of the internal energy defect Delta E. The shapes found for the non-Maxwell-Boltzmann distributions resulting in non-Gaussian line profiles raise serious arguments against theexistence of hot and superhot H atoms as it has been proposed, at least with those temperatures.Full Text:http://pre.aps.org/abstract/PRE/v82/i3/e035401

Addresses: [Loureiro, J] Univ Tecn Lisbon, Inst Super Tecn, Inst Plasmas & Fusao Nucl, LabAssociado, P-1049001 Lisbon, Portugal[Amorim, J] Lab Nacl Ciencia & Tecnol Bietanol CTBE, BR-13083970 Campinas, SP, Brazil

9. Substrate cleavage pattern, biophysical characterization and low-resolution structure of anovel hyperthermostable arabinanase from Thermotoga petrophila

Author(s): Squina, FM; Santos, CR; Ribeiro, DA; Cota, J; de Oliveira, RR; Ruller, R; Mort, A;Murakami, MT; Prade, RA | E-mail:[email protected] Source: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Volume: 399 Issue:4 Pages: 505-511 | DOI: 10.1016/j.bbrc.2010.07.097 | Published: SEP 3 2010

Abstract: Arabinan is a plant structural polysaccharide degraded by two enzymes: alpha-L-arabinofuranosidase and endo-1,5-alpha-L-arabinanase. These enzymes are highly diversified innature, however, little is known about their biochemical and biophysical properties. We havecharacterized a novel arabinanase (AbnA) isolated from Thermotoga petrophila with uniquethermostable properties such as the insignificant decrease of residual activity after incubation up to 90degrees C. We determined the AbnA mode of operation through capillary zone electrophoresis, which

accumulates arabinotriose and arabinobiose as end products after hydrolysis of arabinan-containingpolysaccharides. Spectroscopic analyses by Far-UV circular dichroism and intrinsic tryptophanfluorescence emission demonstrated that AbnA is folded and formed mainly by beta-sheet structuralelements. In silico molecular modeling showed that the AbnA structure encompasses a five-bladedbeta-propeller catalytic core juxtaposed by distorted up-and-down beta-barrel domain. The low-resolution structure determined by small angle X-ray scattering indicated that AbnA is monomeric insolution and its molecular shape is in full agreement with the model. (C) 2010 Elsevier Inc. All rightsreserved.Full Text:http://www.sciencedirect.com/science/article/pii/S0006291X10014178

Addresses: [Squina, FM; Ribeiro, DA; Cota, J; Ruller, R] CNPEM, Lab Nacl Ciencia & TecnolBioetanol CTBE, Campinas, SP, Brazil

[Santos, CR; de Oliveira, RR; Murakami, MT] CNPEM, Lab Nacl Biociencias LNBio, Campinas, SP,Brazil[Cota, J] Univ Estadual Campinas, Dept Ciencias Alimentos, BR-13081970 Campinas, SP, Brazil[Mort, A] Oklahoma State Univ, Dept Biochem, Stillwater, OK 74078 USA[Prade, RA] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 USA

8. Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies ofthe catalytic domain of a hyperthermostable endo-1,4-beta-d-mannanase from Thermotogapetrophila RKU-1
http://pre.aps.org/abstract/PRE/v82/i3/e035401http://pre.aps.org/abstract/PRE/v82/i3/e035401http://pre.aps.org/abstract/PRE/v82/i3/e035401mailto:[email protected]:[email protected]:[email protected]://www.sciencedirect.com/science/article/pii/S0006291X10014178http://www.sciencedirect.com/science/article/pii/S0006291X10014178http://www.sciencedirect.com/science/article/pii/S0006291X10014178http://www.sciencedirect.com/science/article/pii/S0006291X10014178mailto:[email protected]://pre.aps.org/abstract/PRE/v82/i3/e035401


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Author(s): Santos, CR; Squina, FM; Navarro, AM; Ruller, R; Prade, R; Murakami, MT | E-mail:[email protected] Source: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY ANDCRYSTALLIZATION COMMUNICATIONS Volume: 66 Pages: 1078-1081 | DOI:10.1107/S1744309110029131 | Published: SEP 2010

Abstract: Endo-1,4-beta-d-mannanases play key roles in seed germination and fruit ripening andhave recently received much attention owing to their potential applications in the food, detergent andkraft pulp industries. In order to delineate their structural determinants for specificity and stability, X-ray crystallographic investigations combined with detailed functional studies are being performed. Inthis work, crystals of the catalytic domain of a hyperthermostable endo-1,4-beta-d-mannanase fromThermotoga petrophila RKU-1 were obtained from three different conditions, resulting in twocrystalline forms. Crystals from conditions with phosphate or citrate salts as precipitant (CryP)belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 58.76, b = 87.99, c = 97.34 A,while a crystal from a condition with ethanol as precipitant (CryE) belonged to space groupI2(1)2(1)2(1), with unit-cell parameters a = 91.03, b = 89.97, c = 97.89 A. CryP and CryE diffracted toresolutions of 1.40 and 1.45 A, respectively.Full Text:http://journals.iucr.org/f/issues/2010/09/00/hc5111/index.html

Addresses: [Santos, CR; Navarro, AM; Murakami, MT] Ctr Nacl Pesquisa Energia & Mat, Lab NaclBiociencias LNBio, BR-13083970 Campinas, SP, Brazil[Squina, FM; Ruller, R] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol CTBE,BR-13083970 Campinas, SP, Brazil[Prade, R] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 USA

7. Sustainability of the production of ethanol from sugarcane: The Brazilian experience

Author(s): Leal, MRLV; Walter, AD | E-mail:[email protected] Source: INTERNATIONAL SUGAR JOURNAL Volume: 112 Issue: 1339 Pages: 390-396 |

Published: JUL 2010

Abstract: The increase in production of liquid biofuels from different feedstocks is causing concern forpotential importers and users, as an alternative to reduce greenhouse gas (GHG) emissions. Thesustainability of the whole production chain of each biofuel is under review. Some countries, especiallyin the European Union (EU), are jumping ahead in the process of preparing certification procedures toassure the sustainability of the different alternatives under consideration; this is the beginning of theprocess to show that all biofuels are not equal. Ethanol from sugarcane has demonstrated, so far,superiority over other biofuels (ethanol from grains or sugar beet, and biodiesel from soybeans orrapeseed), but the demonstration process must be clear and based on measurable parameters.Surplus power generation is an important point to improve the energy and Green House Gas (GHG)balances, increasing the value of ethanol in the GHG abatement process when it displaces the use of

gasoline as a transport fuel. Brazil as a major producer and exporter of ethanol from sugarcane isworking hard to demonstrate the sustainability of its ethanol, using reliable information, wellestablished procedures and participating in the worldwide effort to produce certification standards. Netenergy ratios above eight and GHG abatement efficiency better than 80% for the Brazilian productionand use chain have been demonstrated. This paper presents a comprehensive assessment of thisBrazilian work toward the demonstration of sugarcane ethanol sustainability, offering some insightsand lessons for other countries starting in this process.

Addresses: [Leal, MRLV; Walter, AD] Ctr Sci & Technol Bioethanol, Campinas, SP, Brazil[Walter, AD] Univ Estadual Campinas, Campinas, SP, Brazil
mailto:[email protected]:[email protected]://journals.iucr.org/f/issues/2010/09/00/hc5111/index.htmlhttp://journals.iucr.org/f/issues/2010/09/00/hc5111/index.htmlhttp://journals.iucr.org/f/issues/2010/09/00/hc5111/index.htmlmailto:[email protected]:[email protected]:[email protected]:[email protected]://journals.iucr.org/f/issues/2010/09/00/hc5111/index.htmlmailto:[email protected]


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6. Low-energy elastic electron scattering from furan

Author(s): Khakoo, MA; Muse, J; Ralphs, KK; da Costa, RF; Bettega, MHF; Lima, MAP | E-mail:[email protected] Source: PHYSICAL REVIEW A Volume: 81 Issue: 6 Article Number: 062716 | DOI:

10.1103/PhysRevA.81.062716 | Published: JUN 30 2010

Abstract: We report normalized experimental and theoretical differential cross sections for elasticelectron scattering by C(4)H(4)O (furan) molecules from a collaborative project between severalBrazilian theoretical groups and an experimental group at California State Fullerton, USA. Themeasurements are obtained by using the relative flow method with helium as the standard gas and athin aperture target gas collimating source. The relative flow method is applied without the restrictionimposed by the relative flow pressure condition on helium and the unknown gas. The experimentaldata were taken at incident electron energies of 1, 1.5, 1.73, 2, 2.7, 3, 5, 7, 10, 20, 30, and 50 eV andcovered the angular range between 10 degrees and 130 degrees. The measurements verify observedpi* shape resonances at 1.65 +/- 0.05eV and 3.10 +/- 0.05 eV scattering energies, in good agreementwith the transmission electron data of Modelli and Burrow [J. Phys. Chem. A 108, 5721 (2004)].

Furthermore, the present results also indicated both resonances dominantly in the d-wave channel.The differential cross sections are integrated in the standard way to obtain integral elastic crosssections and momentum transfer cross sections. The calculations employed the Schwingermultichannel method with pseudopotentials and were performed in the static-exchange and in thestatic-exchange plus polarization approximations. The calculated integral and momentum transfercross sections clearly revealed the presence of two shape resonances located at 1.95 and 3.56 eVand ascribed to the B(1) and A(2) symmetries of the C(2v) point group, respectively, in very goodagreement with the experimental findings. Overall agreement between theory and experimentregarding the differential, momentum transfer, and integral cross sections is very good, especially forenergies below 10 eV.Full Text:http://pra.aps.org/abstract/PRA/v81/i6/e062716

Addresses: [Khakoo, MA; Muse, J; Ralphs, K] Calif State Univ Fullerton, Dept Phys, Fullerton, CA92834 USA [da Costa, RF] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Sao Paulo,Brazil[Bettega, MHF] Univ Fed Parana, Dept Fis, BR-81531990 Curitiba, Parana, Brazil[Lima, MAP] Univ Estadual Campinas, Inst Fis Gleb Wataghin, BR-13083970 Campinas, SP, Brazil[Lima, MAP] CNPEM, Lab Nacl Ciencia & Tecnol Bioetano CTBE, BR-13083970 Campinas, SP, Brazil

5. Low-energy electron collisions with pyrrole

Author(s): de Oliveira, EM; Lima, MAP; Bettega, MHF; Sanchez, SD; da Costa, RF; Varella, MTD | E-mail:[email protected]

Source: JOURNAL OF CHEMICAL PHYSICS Volume: 132 Issue: 20 Article Number: 204301 | DOI:10.1103/PhysRevA.81.062716 | Published: MAY 28 2010

Abstract: We report cross sections for low-energy elastic electron scattering by pyrrole, obtained withthe Schwinger multichannel method implemented with pseudopotentials. Our calculations indicate pi(*)shape resonances in the B(1) and A(2) symmetries, and two sigma(*) resonances in the A(1)symmetry (the system belongs to the C(2v) point group). The present assignments of pi(*) resonancesare very close to those previously reported for the isoelectronic furan molecule, in agreement withelectron transmission spectra. The lowest-lying sigma(*) anion is localized on the N-H bond andprovides a dissociation coordinate similar to those found in the hydroxyl groups of organic acids and
mailto:[email protected]:[email protected]://pra.aps.org/abstract/PRA/v81/i6/e062716http://pra.aps.org/abstract/PRA/v81/i6/e062716http://pra.aps.org/abstract/PRA/v81/i6/e062716mailto:[email protected]:[email protected]:[email protected]:[email protected]://pra.aps.org/abstract/PRA/v81/i6/e062716mailto:[email protected]


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alcohols. This sigma(*)(NH) resonance overlaps the higher-lying pi(*) resonance (possibly both pi(*)states) and could give rise to direct and indirect dissociation pathways, which arise from electronattachment to sigma(*) and pi(*) orbitals, respectively. The photochemistry of pyrrole and 9-H adenineis similar, in particular with respect to the photostability mechanism that allows for the dissipation ofthe photon energy, and we believe pyrrole would also be a suitable prototype for studies ofdissociative electron attachment (DEA) to DNA bases. We point out the connection between themechanisms of photostability and DEA since both arise from the occupation of sigma(*) and pi(*)

orbitals in neutral excited states and in anion states, respectively.Full Text:http://link.aps.org/doi/10.1103/PhysRevA.81.062716

Addresses: [da Costa, RF; Varella, MTD] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170Sao Paulo, Brazil [de Oliveira, EM; Lima, MAP] Univ Estadual Campinas, Inst Fis Gleb Wataghin, BR-13083970 Campinas, SP, Brazil[Bettega, MHF; Sanchez, SD] Univ Fed Parana, Dept Fis, BR-81531990 Curitiba, Parana, Brazil[Lima, MAP] CNPEM, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, BR-13083970 Campinas, SP,Brazil

4. The conserved and divergent roles of carbonic anhydrases in the filamentous fungi

Aspergillus fumigatus and Aspergillus nidulans

Author(s): Han, KH; Chun, YH; Figueiredo, BDP; Soriani, FM; Savoldi, M; Almeida, A; Rodrigues, F;Cairns, CT; Bignell, E; Tobal, JM; Goldman, MHS; Kim, JH; Bahn, YS; Goldman, GH; Ferreira, MED |E-mail:[email protected] Source: MOLECULAR MICROBIOLOGY Volume: 76 Issue: 3 Pages: 802-802| DOI: 10.1111/j.1365-2958.2010.07058.x | Published: MAY 2010

Abstract: Carbon dioxide (CO2) and its hydration product bicarbonate (HCO3-) are essential

molecules in various physiological processes of all living organisms. The reversible interconversionbetween CO2 and HCO3

- is in equilibrium. This reaction is slow without catalyst, but can be rapidlyfacilitated by Zn2+-metalloenzymes named carbonic anhydrases (CAs). To gain an insight into the

function of multiple clades of fungal CA, we chose to investigate the filamentous fungi Aspergillusfumigatus and A. nidulans. We identified four and two CAs in A. fumigatus and A. nidulans,respectively, named cafA-D and canA-B. The cafA and cafB genes are constitutively, stronglyexpressed whereas cafC and cafD genes are weakly expressed but CO2-inducible. Heterologousexpression of the A. fumigatus cafB, and A. nidulans canA and canBgenes completely rescued thehigh CO2-requiring phenotype of a Saccharomyces cerevisiaence103mutant. Only the cafAcafBand canB deletion mutants were unable to grow at 0.033% CO2, of which growth defects can berestored by high CO2. Defects in the CAs can affect Aspergilli conidiation. Furthermore, A.fumigatuscafA, cafB, cafC, cafDand cafAcafBmutant strains are fully virulent in a low-dosemurine infection.Full Text:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2010.07058.x/full

Addresses: [Figueiredo, BDP; Soriani, FM; Savoldi, M; Tobal, JM; Goldman, GH; Ferreira, MED] UnivSao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo, Brazil[Han, KH; Chun, YH] Woosuk Univ, Dept Pharmaceut Engn, Wonju, South Korea[Almeida, A; Rodrigues, F] Univ Minho, Sch Hlth Sci, Life & Hlth Sci Res Inst ICVS, Braga, Portugal[Cairns, CT; Bignell, E] Univ London Imperial Coll Sci Technol & Med, Dept Microbiol, London,England[Goldman, MHS] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Sao Paulo, Brazil[Kim, JH] Soongsil Univ, Dept Bioinformat, Seoul, South Korea [Bahn, YS] Yonsei Univ, Ctr FungalPathogenesis, Dept Biotechnol, Seoul 120749, South Korea
http://link.aps.org/doi/10.1103/PhysRevA.81.062716http://link.aps.org/doi/10.1103/PhysRevA.81.062716http://link.aps.org/doi/10.1103/PhysRevA.81.062716mailto:[email protected]:[email protected]:[email protected]://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2010.07058.x/fullhttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2010.07058.x/fullhttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2010.07058.x/fullhttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2010.07058.x/fullmailto:[email protected]://link.aps.org/doi/10.1103/PhysRevA.81.062716


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3. Electron collisions with alpha-D-glucose and beta-D-glucose monomers

Author(s): da Costa, RF; Bettega, MHF; Varella, MTD; Lima, MAP | E-mail:[email protected] Source: JOURNAL OF CHEMICAL PHYSICS Volume: 132 Issue: 12 Article Number: 124309| DOI:10.1063/1.3369646 | Published: MAR 28 2010

Abstract: The development of new alternative routes for production of second generation ethanolfrom sugarcane biomass poses a challenge to the scientific community. Current research in this fieldaddresses the use of a plasma-based pretreatment of the lignocellulosic raw material. With the aim toprovide a theoretical background for this experimental technique we investigate the role of low-energyelectrons from the plasma in the rupture of the matrix of cellulosic chains. In this paper, we reportcalculated cross sections for elastic scattering of low-energy electrons by the alpha- and beta-D-glucose monomers. The calculations employed the Schwinger multichannel method withpseudopotentials and were carried out at the static-exchange and static-exchange plus polarizationlevels of approximation. Through the comparison of the results obtained with inclusion of polarizationeffects we discuss the influence of the different conformations of the hydroxyl group linked to theanomeric carbon on the resonance spectra of these molecules. Resonant structures appearing at

different energies for alpha- and beta-glucose at the low-energy regime of impact energies can beunderstood as a fingerprint of an "isomeric effect" and suggest that distinct fragmentation mechanismsproceeding via sigma(*) shape resonances may become operative depending on the glucose anomerunder consideration. For energies above 15 eV the integral elastic cross sections are very similar forboth monomers. Differential cross sections for the glucopyranose anomers considered in this work aretypically dominated by a strong forward scattering due to the molecules' large electric dipole momentsand, for energies close to the resonances' positions, they display particular features at theintermediate angular region, notably a pronounced f-wave scattering pattern, that are probablyassociated with the presence of those structures.Full Text:http://jcp.aip.org/resource/1/jcpsa6/v132/i12/p124309_s1?isAuthorized=no

Addresses: [da Costa, RF; Varella, MTD] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170

Sao Paulo, Brazil[Bettega, MHF] Univ Fed Parana, Dept Fis, BR-81531990 Curitiba, Parana, Brazil[Lima, MAP] Lab Nacl Ciencia & Tecnol Bioetanol, BR-13083970 Campinas, SP, Brazil[Lima, MAP] Univ Estadual Campinas, Inst Fis Gleb Wataghin, BR-13083970 Campinas, SP, Brazil

2. Identification of possible targets of the Aspergillus fumigatus CRZ1 homologue, CrzA

Author(s): Soriani, FM; Malavazi, I; Savoldi, M; Espeso, E; Dinamarco, TM; Bernardes, LAS; Ferreira,MES; Goldman, MHS; Goldman, GH | E-mail:[email protected] Source: BMC MICROBIOLOGY Volume: 10 Article Number: 12 | DOI: 10.1186/1471-2180-10-12 |Published: JAN 15 2010

Abstract: Background: Calcineurin, a serine/threonine-specific protein phosphatase, plays animportant role in the control of cell morphology and virulence in fungi. Calcineurin regulateslocalization and activity of a transcription factor called CRZ1.Recently, we characterize Aspergillus fumigatus CRZ1 homologue, AfCrzA. Here, we investigatewhich pathways are influenced by A. fumigatus AfCrzA during a short pulse of calcium bycomparatively determining the transcriptional profile of A. fumigatus wild type and.AfcrzA mutantstrains.Results: We were able to observe 3,622 genes modulated in at least one timepoint in the mutant whencompared to the wild type strain (3,211 and 411 at 10 and 30 minutes, respectively). Decreased
mailto:[email protected]:[email protected]://jcp.aip.org/resource/1/jcpsa6/v132/i12/p124309_s1?isAuthorized=nohttp://jcp.aip.org/resource/1/jcpsa6/v132/i12/p124309_s1?isAuthorized=nohttp://jcp.aip.org/resource/1/jcpsa6/v132/i12/p124309_s1?isAuthorized=nomailto:[email protected]:[email protected]:[email protected]:[email protected]://jcp.aip.org/resource/1/jcpsa6/v132/i12/p124309_s1?isAuthorized=nomailto:[email protected]


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mRNA abundance in the Delta crzA was seen for genes encoding calcium transporters, transcriptionfactors and genes that could be directly or indirectly involved in calcium metabolism. Increased mRNAaccumulation was observed for some genes encoding proteins involved in stress response.AfCrzA overexpression in A. fumigatus increases the expression of several of these genes. Thedeleted strain of one of these genes, AfRcnA, belonging to a class of endogenous calcineurinregulators, calcipressins, had more calcineurin activity after exposure to calcium and was lesssensitive to menadione 30 mu M, hydrogen peroxide 2.5 mM, EGTA 25 mM, and MnCl(2) 25 mM. We

constructed deletion, overexpression, and GFP fusion protein for the closely related A. nidulansAnRcnA.GFP::RcnA was mostly detected along the germling, did not accumulate in the nuclei and its locationis not affected by the cellular response to calcium chloride.Full Text:http://www.biomedcentral.com/1471-2180/10/12

Addresses: [Soriani, FM; Savoldi, M; Dinamarco, TM; Bernardes, LAS; Ferreira, MES; Goldman, GH]Univ Sao Paulo, Ctr Ciencia & Tecnol Bioetanol, BR-14040903 Ribeirao Preto, Brazil[Soriani, FM; Savoldi, M; Dinamarco, TM; Bernardes, LAS; Ferreira, MES; Goldman, GH] Univ SaoPaulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, Brazil[Goldman, MHS] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040903Ribeirao Preto, Brazil

[Malavazi, I] Univ Fed Sao Carlos, Dept Genet & Evolucao, CCBS, BR-13565905 Sao Carlos, SP,Brazil[Espeso, E] CSIC, Ctr Invest Biol, Dept Mol Microbiol, Madrid 28040, Spain

1. Simulation of ethanol production from sugarcane in Brazil: economic study of anautonomous distillery

Author(s): Dias, MOS (Dias, Marina O. S.); Cunha, MP (Cunha, Marcelo P.); Jesus, CDF (Jesus,Charles D. F.); Scandiffio, MIG (Scandiffio, Mirna I. G.); Rossell, CEV (Rossell, Carlos E. V.); Maciel,R (Maciel Filho, Rubens); Bonomi, A (Bonomi, Antonio) | E-mail:[email protected] Source: 20TH EUROPEAN SYMPOSIUM ON COMPUTER AIDED PROCESS ENGINEERING -

Book Series: Computer-Aided Chemical Engineering Volume: 28 Pages: 733-738 | DOI:10.1016/S1570-7946(10)28123-3 | Published: 2010

Abstract: Simulation of the production of ethanol from sugarcane in an autonomous distillery wascarried out using software SuperPro Designer and electronic spreadsheet. Analysis of the ethanolproduction costs was performed for different production scenarios, considering improvements on theenergy production from sugarcane bagasse and the selling of surplus electricity. It was verified thatselling of surplus electricity positively influences the ethanol production costs.Full Text:http://www.sciencedirect.com/science/article/pii/S1570794610281233

Addresses: [Dias, MOS; Cunha, MP; Jesus, CDF; Scandiffio, MIG; Rossell, CEV; Bonomi, A] CTBEBioethanol Sci & Technol Natl Lab, BR-13083970 Campinas, SP, Brazil
http://www.biomedcentral.com/1471-2180/10/12http://www.biomedcentral.com/1471-2180/10/12http://www.biomedcentral.com/1471-2180/10/12mailto:[email protected]:[email protected]:[email protected]://www.sciencedirect.com/science/article/pii/S1570794610281233http://www.sciencedirect.com/science/article/pii/S1570794610281233http://www.sciencedirect.com/science/article/pii/S1570794610281233http://www.sciencedirect.com/science/article/pii/S1570794610281233mailto:[email protected]://www.biomedcentral.com/1471-2180/10/12

Documents

CTBE Publications: 2010