Csir-net June 2011 (Question Paper) Part c(II) Life Science

8/6/2019 Csir-net June 2011 (Question Paper) Part c(II) Life Science

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90. siRNAs andmiRNAs are used or achievinggenesilencing. Although, major

stepsare similar therearedistinct differencesn the key playersof the two

processingpathways. Following statementselate to some characteristic eatures

, of genesilencing.

A) Both siRNAs andmiRNAs areprocessed y cytoplasmicendonuclease

Dicer.

B) 'Drosha'isneeded or processingmiRNAs andprecursor iRNAs.

C) Both siRNAs andmiRNAs show associationwith Argonauteprotein.

D) Both heprocessingathwaysnvolveRISCcomplex.

Which of the following combinationss NOT correct?

(1)A and C (2) C andD (3) A and B (4) D andA

91. Glucose s mobilized n musclewhen epinephrine ctivatesG..s. n an experiment

in which musclecellswerestimulatedwith epinephrine, lucosemobilizationwas

observedeven afterwithdrawal of epinephrine. his could be

( ) due o thepresencef a cAMP phosphodiesterasenhibitor.

(2) very low ratesof cyclic AMP formation. .(3) due o thepresence f a cAMP phosphodiesterasectivator.

(4) due o the absence f protein kinaseA'

92. In eukaryoticchromatin, 30 nm fiber (solenoid)can open up to give rise to two

kinds of chromatin.In one type (A), the promoterof a genewithin the open

chromatin s occupiedby a nuclesonne hereas n the other (B), the pr'omoter s

occupied y histoneHl.

The following possibilitiesaresuggested.

A) Thegene n (A) is repressed.

B) Thegene n (B) is repressed.

C) The geno n (A) is active.

D) The gene n (B) is active.

Which of the following sets s correct?

(1)AandD (2)AandB (3)BandD (4)CandD

S/10 Tele/1{-3AE-2



93.

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IntracellLrlarathogensike M1'cobcteriu,Srtlmonellu,eishmania ndListeritt

survive n rnacrophagesy.modulating ostcellularmachinery.n order o study

the ateof thesentracellular athogensn rnacrophages,ellswere abelledwith

lysotracker ed and nfectedwith GFP-labelled rganisms.After 2 hoursat 37oC.

cells vere ixed,stained ith anti-transferrineceptor ntibody ndprobed vith

secondary ntibody onjugated-blueyes.Cellswerevierved ndercotlfocal

microscope.

Obsen'ation:GFP-iabelled t,cohucterin,alntonellaandListerir,twere ocalizecin

tlresanre ompaftmentabelledwith bluedyes,u'hereas FP-l,eishmtutitt

colocalizewith recl abelled omparlnrent.Whichof the ollorving tateulents true

basecl n these bsen,ations?

(1) M.t,cobucteria,aln'tonellun'J isteria esiden the ysosonles.

(2 ) Leishnrcmiaesiden lysosomeike compartment

(3) Leishnruniaesiden a comparlment hicirbears haracteristicsf earlv

endoc ticcolnpartment.(4 ) lVh,cobut:terict,alntonella ndListeria yse thephagosomalllerrtbrtrtle

and eside n cytosol.

Macrophages ere collected ront BALBic nrice,CD4O-deficientlt icc. CD86-

ileficientmice and CAM-1-deficient rice.Tltese nacrophages\ere go-ctt l l t tr. 'd

rvith LCMV peptide-specific ceils in presence f the LCMV peptide br three

days. The iells were recovered and co-cultured with BALB/c-derivecl

pracrophagesn presence f the peptide.During the last tr,r.'elveour of 1heco-

;ffi;;tfi-rfry-iJi". rvasadded o the cultures. he cellsn'creharvcsted nd

IJ-

thymidine ncorporation as assessed.hehighest rtcorporatioll,r sobs.-t'r.d tl

(1 ) BALB/c macrophage-Tel l co-culture.(2) CD40-deficientmacrophage-Tceilco-culture.(3) CD86-deficientmacrophage-Tceilco-culture.(4) ICAM-1-deficientmacrophage-Tcellco-culture.

Genctic tudies 'emonstratedhatTBP mutant el l extracts redeficientn

tralscriptionof genes rom all threepromoters iz. class ,II and IL Follori'ing

statententsescribe haracteristiceatures f TBP.

A) TBP is considered s an universalbasal ranscription actor.

B) TBP is not requiredor transcription f archaeal enes'

C) TBP is involved n recognizing ATA box'

D) TBP operates t al l pronlotersegardlessf theirTATA content.

Which of the bllowins cornbinationss NOT correct

9.1.

95.

(1) A andD (2) C andD (3) B andD (4) A arrdC



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96. CAMPsignallingplSysa very important ole in the development nddifferentiation

of Dictyostetiui'diicoideum. This morphogen s synthesized y different adenyl

cyclases xpressed t differentstages f its life cycle' The following statementsA-

O; refer o ihe effectof mutations n differentadenylcyclasegenes:

A) aca deficient ellscanbe allowed o aggregate y exposing hem o

Pulses f cAMP.

B) qcb deficientcellswould form normal fruiting bodiesand the spores

,cangerminatewhenexposedtofavourablecondi t ions'

C) acg ieficient cellsdeveiopnormally and he sporesgerminate n

thesPore ead tself.

D) .por", formed from the acg deficientcells will germinate rrespective

of theosmoticconditions'

Whichof theabovestatements reconect?

The otalvariancen aphenotypic haracteranbe split nto two cornponents

geneticVc) andenuironmentaiVr). Theheritability f aphenotft:llTl:T9t

!*pr.rr.d quantitativell,aseritability oefficienth2)which s calculatedsh' :

(1)A andD. (2)A only. (3 )A andB. (a)C andD.

97. Fill in theblanlis a,b, c andd) in the following statements ith a proper

combinationof m, n, o andP.

\&herein m represents longer

n rePresentsshorter

o rePresentsPrevents

P rePresents induces

Short day (sD) plants flower when night lengthsare a than a critical dark

period. tntemrption of the dark period by a brief light treatment-b-flowering

in SD plants.Long day (LD) plants flower when night length is c than a

critical period. Stio.tettitrg of- the night with a brief light treatment d

flowering in LD Plants.

(1)(2)

(3)(4)

monp

npmo

nompmpno

98.

(1) Vc - VE (3)# (4)#

Sl10 Tete/l1-3AE-3A

(2) VE vc



99.

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Mutations in CONSTAffS(CO) of Arabidopsis thalianaphenotype' Transcript revels"l

ao;;r" determined nseedlings.Which of the followin! *ouiO fitefv represent

results n late flowerinslong day and short daithe transcriptprofiie of

Long-day

seedlings(1)

-

Short-day

seedlings

a)(3)

-r

(4) |-E

k

100' Followingaresomestatementsor synthesis f secondarymetabolitesn planis.

A) Terpeaesare syntheszed,byshikimic acidpathway andmevalonic cidpathway.- r* .

fitt?f i: :ffi ;;t,fffl;"ntainigcompoundsndr s nthesizedy

c) phenoliccompounds'aresyntheszed.byshikimicacidpathwayandevaionic cidpathwav.D) Both arkalord-r-yj terplnesaresynthesized y mevaronicacidathway andMEp putlrro,uy.

srhich oneof thefoilowing combinations f theabovesraiements true?

(1 ) A andD (2) A and C (3) B andC (4) B andD101' During earry creavageof caeno.rabtlitis regansembryos,eachasl,rnmetricarivisionproduces ne oundercelJ- hichpr"&;;; d"ifferentiateddescendantsndne stemcelr' The very first ceil divisiont;J;;; one anterior oundercell,amelyAB andont pottt'ior stemcell,namelypi. wrr"n these lastomeresre

f l'"Jil:ll1'ffffii'j"3;g,:lg:i,::,"#i *nn". ihd veopmnt,n

(r) tt r"'l::rld dwelop autonomousryhire heAB wourdshowonditionaldevelopment.(2) p1 cellswould showconditionar evelopmentwhile AB wourdshowutonomous evelopment.(3) Both wourdshowautono*"u, specification nd esurt n mosaicdevelopment.(4)

}JJil"J:Xshowconditionalpecificationndesulrnregurative

SJ10Teieill-3AE-38



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102. ln caseof seaurchin,which of the following is theeorrectsequence f events

takingplaceduing the interactionof spermand egg?

(1) Chernoattracticn f sperrn o theeggby solublemolecules ecreted

by the egg-'exooytosisof thespermacrosomal esicle o releasets

enzymes---rbindingf the spenn o the extracellularmatrix of theegg-+passage f sperm hrough this extracellularmatrix-+fusion of egg

and spermcell membranes.

(2) Chemcattraciionof speryno ihe eggby soluble moleculessecreted y the

egg+binding of the sperm o the extracellular natrix of the egg->

exocytcsis f the spermacrosomal esicle o releasets enzymes-+passage

of sperm hroughthis extracellularmatrix->fusion of eggand spermcell

rnembranes

(4)

Chemoattraction f sperm o the eggby solublemolectilessecreted y the

egg-+binding of the spennto the extracellularmatrix of the egg->passage

of sperm through this extracellular matrix-+exocytosis of ihe spefin

acrcsarnal esicle'to release ts enzymes-+fusion f egg and spermcell

rnembranss.

Chemoattraction f spenn o the eggby solublemoleculessecreted y the

egg-+passage f spermthrough this extracellular matrix-+biriding of the

spemr tc the extracellularmatrix of the egg-'€xocytosis of the sperrn

acrosomalvesicle to release ts enzlmes-+fusion of egg and spermcell

membranes.

103. The time taken for atrial systoleand diastole n a normal heart are u, andt.o

seccnds,espectively. f ventricularsystoleakes u,seconds, aiculatehe

ventriculardiastolic ime (seconds).

(3)

(2)

(4,

(1)

(3)

( t"r*tua)-tu '

(1"6-tur)- to,

( tur-1"6)+tu,

( t".*tr6)xt"t

104. A patient undergoes iver transplantationand during the course of post-operative

treatment, becomessusceptible o infection. The patient can be treated in twodifferent modes and can have alternative outcomes. Which of the following

statementss correct?

Treatrnent ith immunostimulatorymgs educinghe nfectionbut

rejecting he ransplant.(1)

(2)

(3)

(4)

Treatrnent ith immunostimulatoryretaining he ransplant.

drugs educing he nfectionand

Treatmeni with antibiotics reducing the infeetion but retaining the

transplant.Treairvls$twirh antibictics reducing he infesttcn but rejectingthe

tra:lspia:tt.



22

105' Thedependence f the rateof sucrose ptake.with espect o sucrose oncentrationn plant cell was studiedanddataar. ,ho*n in the foitowing graph.

I6

Eo.poJ(t)

nt

Sucrce concentration

From the abovedata t can be inferredthat

(1) the sucroseuptake s energy ridependentandno specialcarrier is

involved.the sucrose ptake s energydependentand a specialcarrier s involved.at lower concentration f sucrosetreup1at. of .o..or.lr;;"rgydependentand carriermediated.at higherconcentrationofsucrosethe uptake s energydependentandcarriermediated.

(2)(3)

(4)

105' DruingepisodesfAo*i." in plants, ymvateproducedn glycolysiss initiallyermentedo.lactat'e' uring aterstage,heres an ncreasen the ermentationothmoranddecreasen the-fermr.ntulrgno lactateaph"no_"nawhichhelpslarsssurviveanoxia'which of the ollowingsi"t".mu is correctabout hishange

f fermentationlux fromractateowards thanor?(l) Thecytosolic H increases,husactivating oth actate ehydrogenaseandpynrvate decarboxylasectivity.(2) Thecytosolic H inc.eales,husinhibitingactateehydrogenaseactivitya1{.actlyajingymvate ecarboxilasectivity.(3) ThecytosoricH orrirur"r, thus.activatirig-i"fi,ractatedehydrogenaseandpynrvate decarboxylasectivity.

------

(4) Thecytosolic H decrea"ses,hus nhibiting lactate ehydrogenasendctivatingyruvate ecarboxyrasectivitv]

107. Bacteriaften,foreign,*;;;,::Hili:ffi"rL:::,Tli,F:;,;:"fif.ffiT:,rT#i;ittontent, s comparedo 'native'genes.Supposehe genomicGC contentof aacteriums 40%.GeneA ortrusorganism'contains1000 ases ith 225G and15c. Another ene-Bf rengthoobur.r.onr*n.160G and 40c. whichonef the ollowingwouldbe helnost cceptableypothesisgiven hatx2: 3.g41 t.05 ignificanceevel)?

(1 ) A: native,B: foreign

(3) A: foreign,B: foreicn

(2) A: foreign,B: native

(a) A: native,B: native



23

108. lnjectionof nogginmRNA into a l-cell, uV-irradiated embryosof frog completely

rescuesdorsal"dirr"lop*ent

and allows the formation of a completeembryo' Some

of the following statementsA-D) could possibly explainthis observation.

A) Noggin is a secretedprotein which inducesfursal ec'to'dermo form

neural tissueand it dorsalizesthe mesodermcells which worildotherwise contribute to ventral mesoderm'

B) Noggrn binds directly to BMP4 andBMP2 thrs prerrentingcomplex

formation witr their recePtors'

C) Noggin along with other moleculesprevent BMP from binding to an{d

inducing ectodermand mesodermcellsnear he organizer.

D) Noggin-is a secretedprotein which inducesftle dorsal ectodermto form

the epidermisand t ventralizes he mesodermcellswhich would

otherwisecontribute o dorsalmesoderm'

Which of the abovestatements recorrect?

(1) A, B andC. (2) A andB. (3) B andC. (a)A andD

109. AhnosphericCOzcsrtains the naturallyoccurringstablecarbon sotopes2Cand

'rC in ilre proportionof 98.9% and .lYo,respectively.Following aresomeof the

statementsegardingCOz assimilation:

A) Both Cl andC+plantsassimilateess13COzthan

r2COz'

B) Both C: and C+plantsassimilateess2-COzdran

3COz'

Ci C3plantsassimilate esser'CO, than

l2COzas connparedo C4plants'

Di Caplants assimilateesser

'COr thanr2COz:15 ompared o C: plants.

Which onq of the following combinationsof above statementss true ?

(1) A andB (2) A andC (3) C andD (a) A andD

110. Spinalcord of an animal was transectedatCllC2level. The respirationof the

animal stoppedand t neededartificial respiration. However, the heartcontinued

to beat althoughat a slowerrate.Someof the explanations given were:

A) respiration egulatorycentres located n the medulla.

B) respiration egulatory entres locatedabove he Cl/C2 cut'C) heart egulatorycentre s above he C1lC2cut'

D) hearthasautoregulation.

Which oneof the following is mostappropriate

(1)A only.

(3) A. B andD only.

(2)BandConly.

(4) B, C andD only.

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Csir-net June 2011 (Question Paper) Part c(II) Life Science