Crude Fiber 3107

8/3/2019 Crude Fiber 3107

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Crude Fiber



GROUP NUMBER :3 and 4

GROUP MEMBERS :

-Lim Wee Chun 159687

-Gina Yew Swan Jing 157690-Muhammad Naim Muhammad Khatib 158467-Nor Dalila Hashim 156293-Low Jun Ying 156493

-Lim Hui Xian 156432-Ku Izzaira Ku Ishak 159360-Mohd Faisal Abd. Ghani Hisyam 158120



DEFINITION

Analysis of crude fibre involves oven-dryingthe fiber to be analyzed after exposing it to aseries of sulfuric acid and sodium hydroxidesolutions.

What's left is crude fiber: a mixture ofinsoluble fibers that have no nutritional value.

Crude fibre is a measure of the quantity ofindigestible cellulose, pentosans, lignin, and

other components of this type in food.



Crude fiber is also referred as insoluble

fiber.

Crude fiber is different from dietary fiberwhich is a soluble fiber.

Most crude fiber contains one-seventh toone-half dietary fiber.



COMPONENT OF CRUDE FIBRE

Made up primarily of plant structural

carbohydrates such as cellulose andhemicellouse and lignin, which is a highly

indigestible material.

Found in the tissues of plants and cellulose,basically a plant's skeleton.



These components have little food value

but provide the bulk necessary for properperistaltic action in the intestinal tract.

In short, most people need some crudefiber in their diets.

ADVANTAGE OF HAVING CRUDEFIBER



SOURCE OF CRUDE FIBER

Excellent sources of crude fiber include :- Vegetables like leafy greens- Whole grains like whole wheat

- Rye- Beans such as kidney beans- Black beans.

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This method suitable for by-product of

cereal industry.

Overall, in determining the crude fiber thesample characteristics should be considersuch as:

- particle size- defatted or undefatted sample- the rate of heat given to the sample

Crude fiber can be use to evaluatenutrition, manufacturer performance andthe degree of fruit senescence.



• The sample will be hydrolyzed with an aqueousH2SO4. Then, the sample residue will be rinsedwith water and dried in oven for 3 hrs at 105 oC.Then cool in desiccators and repeat the step for

several time until constant weight is obtained.The remaining residue, will be ash at 550 oC ina muffle furnace until completed. The ash isweighed after it is cooled down to room temp.The weight of crude fiber is the differencesbetween the weight of dried sample residue tothe weight of ash.

PRINCIPLE



Aim of experiment :

• To comprehend the determination of crudefibre in food systems.

• To collect,analyze and evaluate theexperimental data.

• To demonstrate the ability to handle theequipment and chemical effeciently.

• To demonstrate the advantages anddisadvantages of various method.



Apparatus and Materials

• Materials:

•

Sulphuric Acid : 0.25N

• Sodium Hydroxide :0.313 N

•

Alcohol• Sample given

Apparatus :

Muffle furnace (550 C)Buchner funnel/Glass

funnelSpatula

HeaterConical flaskDesiccator

Oven (105 C)

CrucibleFilter paperCondenser

Ashless filter paperNo.541

Aspirator pump



Procedure

Dry the crucible and ashless filter paper(105 C,1hour) ,cool it.

Calculate weight of filter paper,Weigh 2g of defattes sample-conical flask (500ml)

+ boiled sulphuric acid (200ml),

dissolve it (spatula),attach conical flask-Reflux condenser

Boil sample(30 mins),turn the conical flask

every 5mins.

Filter hydrolyzed mixture(filter paper Whatman no.541),Rinse residue (boiled distilled

water)

Place residue-conical flask,+200 ml boiled NaOH,attach

to condenser,boil(30 mins)

Filter hydrolyzed mixtures,

Rinse residue(boiled distilledWater),rinse again(alcohol)

Dry residue+filter paper(105 C),place crucible-muffle

furnace(550 C),place crucible-Dessicator,calculate constantWeight.



• Weight of Sample = W

• Weight of filter paper without ash = K

• Wt. crucible + filter paper + dried residue= S

• Wt. crucible + ash = A

•

• Wt. residue without ash

• % crude fiber = × 100

Wt. sample• (S – K) – A

• = × 100

W

Calculation

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Crude Fiber 3107