CORRELATION BETWEEN HUMAN VIRAL CORRELATION BETWEEN HUMAN VIRAL CONTAMINATION AND CONTAMINATION AND

PHYSICOCHEMICAL PROFILES IN A PHYSICOCHEMICAL PROFILES IN A SURFACE WATER LAGOONSURFACE WATER LAGOON

Célia Regina Monte Barardicelia.barardi@ufsc.br

Laboratório de Virologia Aplicadawww.lvapli.ufsc.br

Universidade Federal de Santa Catarina, Brasil

Peri Lagoon: Florianópolis, Santa Catarina, Brazil

Surface area of 5.7 km²

Water is distributed to the inhabitants (110,000 people)

Recreational activities are allowed in the lagoon

More than 2,000 visitors/spring and summer seasons

Gastroenteritis, diarrhea, and other diseases can be caused by

viruses transmitted by fecal/urino-oral route

HAdV – RVA – HAV - JCPyV

Introduced into aquatic environments by

human – industrial and agricultural activities

Studies indicate these viruses as bioindicators of environmental contamination

Introduction

Evaluate the presence of HAdV, JCPyV, HAV and RVA in the largest source of drinking

water of the city of Florianópolis, Santa Catarina,

Brazil (Peri Lagoon);

Investigate their correlation to the water’s

physicochemical parameters and seasonal distribution of

these viruses;

Evaluate the integrity and infectivity of detected HAdV.

Objectives

Site 1: Center of the lagoon

Site 2: Preserved environment

Site 3: Degraded environment

Site 4: Where water is collected for treatment and human consumption and also recreational activities

Enzymatic Assay (Integrity)

Plaque Assay and ICC-et-RT-qPCR (Infectivity)

Methods

HAdV

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HAdV JCPyV HAV RVA0

20

40

60

80

100

Winter Spring Summer Autumn

Vir

al D

istr

ibu

tio

n /

Seaso

n

(%)

1.01000

1.01002

1.01004

1.01006

1.01008

1.01010HAdV

RVA

JCPyV

HAV

Virus: Water Lagoon

Gen

ome

copi

es p

er li

ter

(GC

/L)

(log

10)

95.83% - HAdV (46/48)

64.5% - RV-A (31/48)

20.83% - JCPyV (10/48)

12.5% - HAV (6/48)

• Viral Distribution• The seasonal distribution

Results

Site 1: Center of the lagoon

Site 2: Preserved environment

Site 3: Degraded environment

Site 4: Where water is collected for treatment and human consumption and also recreational activities

Winter: HAdV – HAV - RVA

Summer and Spring: JCPyV

Positive Correlation JCPyV x Nitrite Lagoa do Peri

Jun/1

0

Jul/1

0

Aug/10

Sep/1

0

Oct/1

0

Nov/10

Dec/1

0

Jan/11

Feb/11

Mar

/11

Apr/11

May

/11

1.0100

1.0101

1.0102

1.0103

1.0104

1.0105

1.0106

1.0107

0.0

0.1

0.2

0.3

0.4

NitriteJCPyV

**

JC

Py

V G

C/L

Nitrite (u

g/L

)

Correlation: Virus and physicochemical parameters

Results

Jun/1

0

Jul/1

0

Aug/10

Sep/1

0

Oct/1

0

Nov/10

Dec/1

0

Jan/1

1

Feb/1

1

Mar

/11

Apr/11

May

/11

1.0100

1.0102

1.0104

1.0106

1.0108

Viability (PFU/L) - PATreated (GC/L)

Untreated (GC/L) Viability (CG/L) - ICC-et-RT-qPCR

HA

dV

Integrity and viability of detected HAdV

HAdV: Undamaged: 83.3% (10/12)

Viability by PA: 66.6% (8/12)

Viability by ICC-et-RT-qPCR 83.3% (10/12)

Results

Surface water sources used for human consumption and recreational activities are being

contaminated by human enteric viruses;

The divergence among total genomic copy number, genomic copies of intact virus and

the number of infectious viruses highlights the importance of including molecular and

cell culture techniques during the environmental monitoring of viruses;

ICC-et-RT-qPCR assay was sensible and, fast to detect viral particles infectious (viral

mRNA) in water samples;

Emphasizes the urgency of monitoring and implementation efficient methods for

removing viral.

Conclusions

Financial support:

CNPq Project 470808/2009-8

CTAgro/CNPq/- 25/2010

CAPES: Process : AEX 11419/12-1

Team:

Gislaine Fongaro, MSc studentMariana de Almeida do Nascimento, MSc studentAline Viancelli, PhDDenise Tonetta, MScMaurício Mello Petrucio, PhDCélia Regina Monte Barardi, PhD

celia.barardi@ufsc.brLaboratório de Virologia Aplicada

www.lvapli.ufsc.brUniversidade Federal de Santa Catarina

Gislaine

Mariana N.

Lucas ElMahdy NatáliaDóris

Camila Vanessa Mariana R.