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MCB 186 CIRCADIAN BIOLOGY Clock genes & Biochemical Mechanisms: Kai genes October 10, 2007 J. W. Hastings. CORE CLOCK COMPONENTS IN FEEDBACK LOOPS OF 3 SYSTEMS. TEMPORAL SEPARATION OF CELL BIOCHEMICAL ACTIVITIES IN CYANOBACTERIA ( OXYGEN, NITROGENASE ETC) MITSUI ET AL 1986. - PowerPoint PPT Presentation
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MCB 186CIRCADIAN BIOLOGY
Clock genes & Biochemical Mechanisms: Kai genes
October 10, 2007
J. W. Hastings
TEMPORAL SEPARATION OF CELL BIOCHEMICAL ACTIVITIES IN CYANOBACTERIA (OXYGEN, NITROGENASE ETC) MITSUI ET AL 1986
Cyanobacterial in vivo Rhythm with Bacterial Luciferase as a Reporter
24016814412096720
Hours in Constant Light
2000
4000
6000
8000
12 60 84 108 132 156 180
228
192
204
10000
48
Lu
min
esce
nce
Kondo, Johnson Golden et al., 1993, PNAS
LUCIFERASE REPORTER RANDOMLY INSERTED IN FRONT OF PROMOTERS IN
BACTERIA.
ABOUT 800 LUMINOUS COLONIES ISOLATED
ALL EXHIBITED CIRCADIAN RHYTHMS
ACROPHASES NOT ALL THE SAME
Cyanobacterial Clockworks TTO Model -1998
Circadian rhythms
C
CA
ABB
Protein Interactions
A B C
C
KaiC
B
KaiB
A
KaiA
rhythmicmRNAs
Kai proteins
kaiBCpkaiAp
Ishiura et al., 1998
Cyanobacterial Clockwork Model TRANSCRIPTIONAL CONTROL OF MANY GENES 2003
Xu et al., EMBO J. 22: 2117-2126, 2003
Kai C PHOSPHORYLATION RHYTHM
in vitro
in a SOLUTION CONTAINING only ATP with Kai A, B and C
Phase is not determined by light Nakajima et al, Science, 308: 414-415 (2005 )
In vitro phosphorylation rhythm is temperature compensated
Nakajima, M et al Kondo, T (2005) Reconstitution of circadian oscillation in vitro Science 308: 414-415
In vitro phosphorylation rhythm is temperature compensated
Nakajima, M et al Kondo, T (2005) Reconstitution of circadian oscillation in vitro Science 308: 414-415
Period of phosphorylation rhythm corresponds to mutant period
Tomita, Nakajima, Kondo & Iwasaki (2005) Science 307: 251-254
Tau of in vitro P-oscillation corresponds to mutant tau
Nakajima, M et al Kondo,T (2005) Reconstitution of circadian oscillation in vitro Science 308: 414-415
Simplified model for the posttranslational oscillator Nakajima, M et al Kondo,T (2005) Science 308: 414-415
EVIDENCE THAT TRANSCRIPTION and TRANSLATION are not
NECESSARY for CIRCADIAN OSCILLATIONS
IN CYANOBACTERIA Tomita, Nakajima, Kondo & Iwasaki (2005) No transcription-translation feedback
in circadian rhythm of KaiC phosphorylation Science 307: 251-254
NO RHYTHM OF kaiA or kaiBC mRNAs in DD
Tomita, Nakajima, Kondo & Iwasaki (2005) No transcription-translation feedbackin circadian rhythm of KaiC phosphorylation Science 307: 251-254
RHYTHM OF KaiC PROTEIN PHOSPHORYLATION in DD Tomita, Nakajima, Kondo & Iwasaki (2005) Science 307: 251-254
Period Temperature Compensation of in vivo Rhythm of
KaiC Phosphorylation in DD
Tomita, Nakajima, Kondo & Iwasaki (2005) No transcription-translation feedbackin circadian rhythm of KaiC phosphorylation Science 307: 251-254
Model for the posttranslational oscillator coupled with TTO
Tomita, Nakajima, Kondo & Iwasaki (2005) No transcription-translationfeedbackin circadian rhythm of KaiC phosphorylation Science 307: 251-254