426 Persoonia–Volume36,2016

Coprinus pinetorum

427Fungal Planet description sheets

© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures

FungalPlanet454–4July2016

Coprinus pinetorum G.Moreno,Carlavilla,Heykoop&Manjón, sp. nov. Etymology.Namereflectsthehabitat,humusofPinus halepensis, from whichthisfunguswascollected.

Classification —Agaricaceae, Agaricales, Agaricomycetes.

Cap35–45×25–35mmwhenstillclosed,ellipsoidtosubcylin-drical,becomingrevoluteatmarginwhenmature,firstwhitish,laterwithpinkishtinges,veilfirstfibrillosetoflocculose,thenbreaking up into small and fragile whitish upturned scales, except at centre, that stays smooth and becomes creamy to strawishcreamy.Gillscrowded,firstwhite,andthenpinkishtoblack, the gill-edge whitish, probably due to the presence of numerouscheilocystidia,stronglydeliquescentwhenmature.Stem60–120×10–17mm,whitish,withmovablefragileringonlower part, upperside strawish whitish, underside greyish flesh coloured, with up to 22 mm wide bulbous to subbulbous base, hollow,withcentralstrand.Spores8–11×(5–)5.5–8µmav.8.1–10.6×5.9–6.9µm(4collections),Qav.=1.38–1.62,smooth,ellipsoid, sometimes slightly broadened base, dark black, germ porecentral,upto1–1.5µmdiam.Basidia14–35×10–13µm(excl.sterigmata),sterigmataupto4µminlength,4-spored,hyaline, sometimes with brownish vacuolar pigment toward thebase,surroundedby6–8pseudoparaphyses.Cheilocys­tidia probably present in young specimens but the material studied was always very mature, with the gill-edge completely deliquesced.Pleurocystidianotobserved.Clamp­connections absent, only pseudoclampspresent.Elements of veil35–220×9–35µm,consistingofcylindricalbranchedhyphae,septate,denselypackedandveryvariableinsizeandshape. Habit,Habitat&Distribution —Growinggregariousonbasicsoil under Pinus halepensis.Rareinthestudiedarea.

Typus.sPain,Madrid,RivasVaciamadrid,inhumusofPinus halepensis, 18Nov.2011,M. Martín, L. Rubio­Casas, L. Rubio­Roldán & G. Moreno (holotypeAH44094, ITSsequenceGenBankKU686924,LSUsequenceGenBankKU686907,MycoBankMB815824).

Additional specimens examined.Coprinus pinetorum: sPain, Madrid, RivasVaciamadrid,inhumusofPinus halepensis,18Nov.2011,M. Martín, L. Rubio­Casas, L. Rubio­Roldán & G. Moreno,paratypeAH45797(ITS,LSUsequencesGenBank,KU686925,KU686908);idem,22Nov.2014,M. Martín, paratypeAH45798(ITS,LSUsequencesGenBank,KU686926,KU686909);Almería,SierradelosFilabres,inhumusofPinus halepensis,30Nov.2002,G. Moreno & R. Galán,paratypeAH45815(ITS,LSUsequencesGenBank,KU686927,KU686910). Coprinus comatus: sPain,Alcalá deHenares,Campusuniversitario,FacultaddeBiología,inagarden,15Nov.2008,J. Rejos & G. Moreno,AH44095(ITS,LSUsequencesGenBank,KU686915,KU686898);Madrid,Valdemorillo,inopenareainaforestofQuercus ilex subsp.ballota,10Apr.2010,M. Hinojosa & J.C. Campos,AH45823(ITS,LSUsequencesGenBank,KU686913,KU686896);Madrid,Canillejas, inparkingoftheCaprichoPark,23Mar.2014, J.L. Domingo,AH44089(ITS,LSUsequencesGenBank,KU686914,KU686897);Madrid,LasMatas,on

sideofapath,8May2014,I. Morales,AH45796(ITS,LSUsequencesGen-Bank,KU686916,KU686899);Guadalajara,inagarden,6Dec.2014,J.R. Carlavilla,AH45795(ITS,LSUsequencesGenBank,KU686911,KU686894);AlcaládeHenares,Campusuniversitario,ResidenciaCrusa,inagarden,28Oct.2015,P. Rosario,AH45832(ITS,LSUsequencesGenBank,KU686912,KU686895);idem,4Nov.2015,AH45831(ITS,LSUsequencesGenBank,KU686918,KU686901);AlcaládeHenares,Campusuniversitario,EscuelaPolitécnica, inagarden,6Nov.2015,A. López­Villalba, J.R. Carlavilla & G. Moreno,AH 45830 (ITS, LSU sequences GenBank, KU686917,KU686900). Coprinus littoralis: sPain,Huelva,PlayaCoto deDoñana,NationalParkofDoñana,psammophilousindunes,5Apr.2013,A. Sánchez, holotypeAH45819(ITS,LSUsequencesGenBank,KU686920,KU686903);Huelva,PlayadeDoñana,NationalPark ofDoñana, psammophilous indunes,6Apr.2013,A. Sánchez,AH45860(ITS,LSUsequencesGenBank,KU686921,KU686904),idem,7Apr.2013,AH45859(ITS,LSUsequencesGenBank,KU686922,KU686905).Coprinus vosoustii: sPain, Madrid, Ciu-dadUniversitaria,FacultaddeFarmaciayMedicina,inagarden,13May1976, K. Tabba,AH1284(ITS,LSUsequencesGenBank,KU686919,KU686902);idem,8May1977,G. Moreno,AH556 (ITS,LSUsequencesGenBank,KU686923,KU686906).

Notes—Coprinus pinetorumischaracterisedbyitssmallsize(ascomparedwithC. comatus),itssporeswithslightlybroad- enedbase(8–11×5.5–8µm)andbyfruitingamongneedlesof Pinus halepensis.InourITSphylogeny(MycoBanksupplementarydata)Coprinus pinetorumissignificantlyrelatedtoC. comatus, C. sterquilinus, C. vosoustii and C. littoralis.Theyallbelongtosubsect.Copri­nuss.Uljé(theC. comatusgroup).Coprinus comatus differs from C. pinetorum by its more robust habit and by fruiting on strongly nitrified sites (gardens, roadsides and paths or onlawns).Coprinus sterquilinus differs from C. pinetorum by its solitarybasidiocarps,verylargespores(17–26×10–15µm)andthehabitatondung.Coprinus vosoustii differs from C. pine­ torum by its ovoid cap, thick ochraceous and persistent veil forming a star-shaped layer at centre, and much larger spores (17–20×10–12µm).Coprinus littoralis differs from C. pine­torumbyitslargerbasidiocarps,largerspores(13–20×8–12µm)withslightlyeccentricgermporeandthepsammophiloushabitatinlittoraldunes.Macroscopically, C. spadiceisporus, a very rare species de-scribedfromtheStateofWashingtonbyVandeBogart(1976),is also a C. comatus-likefungus.Nevertheless,itdiffersfromC. pinetorum by fruiting on dung and by its spores with eccen-tricgermpore(VandeBogart1976).AnotherC. comatus-like fungus which resembles Coprinus pinetorum is C. levisticolens.However,C. levisticolens differs from C. pinetorum by its scaly cap,largerspores(11–14.5×7–8µm)witheccentricgermporeand by fruiting on sandy soil under Populus alba and Crataegus spp.(Ludwig&Roux1995).

GabrielMoreno,JuanRamónCarlavilla,MichelHeykoop&JoséLuisManjón,DepartamentodeCienciasdelaVida(ÁreadeBotánica),UniversidaddeAlcalá,E-28805AlcaládeHenares,Madrid,Spain;

e-mail:gabriel.moreno@uah.es,juan.carlavilla@gmail.com,michel.heykoop@uah.es&josel.manjon@uah.es

Colour illustrations.Spain,RivasVaciamadrid,humusofPinus halepen­sis,wheretheholotypewascollected;basidiomataandfruitbodysection,cylindricalhyphaeofveil,4-sporedbasidia,sporesunderLM,smoothsporeswithcentralgermporeunderSEM(fromtheholotype).Scalebars=1cm(basidiomata),10µm(veil,basidia,sporesunderLM),2µm(sporesunderSEM).