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Copan 4N6 Devices for resolving the mysteries of crime scene investigations
Santina Castriciano, Annalisa Gervasoni, Alice Squassina and Michele Rosso. Copan Italia Spa, Brescia, Italy
IntroductionProperly collected, stored and analyzed forensic specimens are important for maintaining nucleic acids integrity for DNA profiling. Copan developed: 1) The 4N6FLOQSwabs® (4N6FS) to facilitate forensic traces collection; 2) The NAO®Basket a semi-permeable basket used with the 4N6FLOQSwabs® for efficient sample release and nucleic acids optimization. 3) The NUCLEIC-CARD™ White (NCW) and COLOR (NUC), a chemically treated paper that allows room temperature (RT) long term storage of DNA from buccal swabs or blood samples for forensic human identification.
ObjectivesThe objectives of this study were a) to compare the 4N6FS, NAO, NCW and NUC to traditional
devices for recovery, storage and preservation ofDNA traces for genetic and crime scene investigations
b) Report forensic cases investigated with 4N6FLOQSwabs ®.
Results 4N6FS recovered almost 100% of hDNA after storage in the presence of contaminants compared to less than 10% with traditional swabs. NAO recovered over 30% more DNA from swab compared to traditional swabs processing. NUC and NCW enabled RT storage of nucleic acids from saliva and blood for genetic DNA profiling. The 4N6FS isolated the DNA profiles from gloves, hat left at the crime scene and the 4N6 nails FS allowed identification of victim and aggressor profiles.
ConclusionsThe 4N6FLOQSwabs® are increasing hDNA detection and preserving hDNA for forensic genetic and crime scene samples collection. The NAO®s are increasing hDNA recovery, especially from touch DNA samples. The NUCLEIC-CARD™ enables RT storage of nucleic acids from saliva and blood samples for DNA profiling and data banking. The 4N6FLOQSwabs® have successful identified the DNA profiles from evidence left on the crime scenes.
4N6FLOQSwabs®
Nucleic Acid Optimizers
MethodsBuccal/saliva and blood traces were prepared on contaminated surfaces and deposited on NUC and NCW. Samples were collected from each trace using 4N6FS and traditional swabs. After collection, swabs were stored in tubes and tested at baseline and after 2 weeks. Buccal/saliva and blood aliquots were used as reference. Swabs were processed as per nucleic acids extraction method and others with the NAOs for nucleic acid recovery. Disks (1.2mm) were punched from both NUC and NCW cards. Case reports included sample collection from gloves, hat, and left behind in 2 robberies and samples collected with 4N6 sub-ungueal from under the nails of a cadaver. All samples were extracted with the PrepFiler Express on the AutoMate Express, amplified with the Human Quantifiler Trio on the 7500 and profiled with the Identifiler Plus on the 3130 Genetic Analyzer (Thermo Fisher).
0
0,2
0,4
0,6
0,8
4N6FS CS
ng/µLogDN
A
Samples
DNAfromtraceswithMixedMicrobes
T0
After2W
0
500
1 2 3
pg/u
Dilutions
DNAfrom4N6FLOQSwabsspikedwithblood with andwithout NAO
Blood
FS/Blood+NAO
FS/Blood 0
1000
2000
1 2 3
Pg/ul
Dilutions
DNAfrom4N6FLOQSwabsspikedwithSalivawith andwithout NAO
Saliva
FS+Saliva+NAOFS+Saliva
Results
The DNA profile of the thief was detected in the sample collected from the gloves witha 4N6FLOQSwab®
The DNA profile of the thief was detected in the sample collected from the hat with a 4N6FLOQSwab®
The DNA profile of both the aggressor and the vitctim were detected in the sample collected under the sub-unguealof the victim with a nail 4N6FLOQSwab®
151413121110987654321
0,9
0,8
0,7
0,6
0,5
0,4
0,3
0,2
0,1
0,0
Donor
DN
A (n
g/ul
)
DNA extracted from 1.2 mm punches (ng/ul) - Saliva samples
151413121110987654321
0,5
0,4
0,3
0,2
0,1
0,0
Donor
DN
A (n
g/ul
)
DNA extracted from 1.2 mm punches (ng/ul) - Blood samples
Regular 4N6FLOQSwab ®
in long or short storage tubes
NUCLEIC-CARD™ White and COLOR
Puritan 25-806 1WC
FDNA
Vienna May 16-17, 2017
00,20,40,60,81
4N6FS CS
ng/µLogDN
A
Samples
DNAobtainedfromeachswabtypefromblood andsalivatraces
Blood
Saliva
