Contribution of the Academy of Sciences

of the Czech Republic to the European Danube Strategy

4th Conference of the Danube Academies Bucharest, Romania, April 9-10, 2013

presented byJan Zima

Environmental Research

• Institute of Experimental Medicine AS CR, Prague http://www.iem.cas.cz

• Biology Centre AS CR, České Budějovice (http://www.bc.cas.cz)

• Institute of Vertebrate Biology AS CR, Brno http://www.ivb.cz

Institute of Experimental Medicine

• Danube Air Nexus – Assessing impacts of carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) on health

• Effects of air pollution on genetic material, on the mechanisms of changes induced by environmental factors as well as modeling the relationships between individual factors (e. g. air pollution vs. life style),

coordinator Dr. R. J. Sram

Project introduced at the meeting

Scientific Support to the Danube Strategy,

Joint Research Centre, Ispra,

Italy, March 22, 2013

RISK FOR HUMAN HEALTH

pregnancy outcome(IUGR, LBW)

act as ED

child morbidity,mental

development

menfertility

cardiovascular diseases, diabetes,

cancer

R. J. Sram 2013

Impact of c-PAHs in polluted air

Institute of Experimental Medicine AS CR, Prague,Czech Republic (Radim J. Sram)

Institute for Environmental Health, Medical University,Wien, Austria (Hanns Moshammer)

Vinca Institute of Nuclear Sciences, Beograd, Serbia(Milena Jovasevic-Stojanovic)

Institute of Medical Research and Occupational Health,Zagreb, Croatia (Alexandra Fucic)

Environmental Health Center, Babes Bolai University,Cluj Napoca, Romania (Eugen S. Gurzau)

Participants

Newborns from normal deliveries (week 38-41) fromnonsmoking mothers

Cord blood collected in the summer and winter season

In each location 100 samples/season will be collected (total = 5 x 2 x 100 = 1 000 samples)

From each group in each location a cohort for further follow-up will be created

These children will be monitored up to 2 years of age (if possible longer) for their morbidity via questionnaires filled out by their pediatrician)

Subjects

Increased exposure to c-PAHs during pregnancy affects gene expression in newborns and their morbidity in early life

DNA damage in newborns is related to exposure to c-PAHs occurring in the ambient air

Gene expression changes induced by the exposure to c-PAHs during pregnancy influence pathways affecting immunity

Expression of genes in cord blood affected by c-PAHs could be used as a new biomarker of exposure to c-PAHs

Hypothesis

R. J. Sram 2013

Gene expression

DNA adducts by 32P-postlabeling

Chromosomal aberrations,micronuclei

Oxidative damage8-oxodG, 15-F2t-isoP, proteins

Metabolomics

PM2.5 Stationary monitoring

c-PAHs Stationary monitoring

VOC Stationary monitoring

Cotinine

Vitamins A, C, E

Interleukins 2, 6, 7, 8

Proposed methods

Biology Centre

Danube Water Nexus; Research effort focused on water (PA 4).

Catchment-scale modeling of nutrient transport and water quality in river and lake systems exposed to climate changes impacts. The scenario results are used in socio-economic analyses for planning mitigation measures for WFD river basin operation plans (PA7).

Environmental Risks (PA5)

Biodiversity, landscapes and quality of air and soil (PA6)

coordinator prof. František Sehnal

Institute of Vertebrate Biology

Danube Water Nexus (PA 4)

Biodiversity, landscapes and quality of air and soil (PA 6)Introduced and invasive fish species in the Danubian river catchment c

coordinator Dr. Pavel Jurajda

Austria

Bulgaria

Germany

Slovakia

Serbia

Romania

Croatia

Ukraine

Hungary

Gobies of the genus Neogobius