Rat Dual Pressure Telemetry 1

Contraction and Relaxation in Aortic Rings

CONTRACTION – RELAXATION IN AORTIC RINGS

CorDynamics can use in vitro approaches to examine effects of test articles on vascular reactivity

Vascular ring option

- constriction or relaxation

- multiple species

- appx. 5mg test article TOTAL

- data available in DAYS

Data

-amplitude, time of contraction

-time to half-contraction (relax)

-average/max activity

CONTRACTION – RELAXATION IN AORTIC RINGS

QC #1: Add 5 µM Phenylephrine

QC # 2: Add 10 µM Acetylcholine

Equilibration : 45 minutes(15-minute washouts)

Thoracic aorta cut into ringsVertical organ bath

Contraction

No contraction

Discard and replace tissues

≥ 30% relaxation

No relaxation

Protocol

CONTRACTION – RELAXATION IN AORTIC RINGS

BL 0.001 0.01 0.03 0.1 0.3 1-20

0

20

40

60

80

100

120

140

160

180

Percent Contraction with Phenylephrineon Rat Thoracic Aorta

Concentration (µM)

% C

ontr

actio

n

CONTRACTION – RELAXATION IN AORTIC RINGS

Phe 0.003 0.01 0.03 0.1 0.3 CCh-25

0

25

50

75

100

125

150

175

200

225

250

Relaxation with Sodium Nitroprusside on Rat Thoracic Aorta Pre-contracted by 400nM of Phenylephrine

DMSO (0.006, 0.004, 0.01, 0.08 and 0.1 %, n=4)

Sodium Nitroprusside(0.003, 0.01, 0.03, 0.1 and 0.3 uM, n=4)

Concentration (µM)

Rel

axat

ion/

g of

aor

ta