Continuous extraction of fatty acids or their conversion to something non-toxic.

http://link.springer.com/article/10.1385%2FJMN%3A16%3A2-3%3A99

http://www.plefa.com/article/S0952-3278(10)00073-6/abstract

http://www.microbialcellfactories.com/content/11/1/41

1. Stahl A, Gimeno RE, Tartaglia LA, Lodish HF: Fatty acid transport proteins: a current view of a

growing family.

Trends Endocrinol Metab 2001, 12(6):266-273. PubMed   Abstract  | Publisher   Full   Text  

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2. Nunn WD, Colburn RW, Black PN: Transport of long-chain fatty acids in Escherichia coli.

Evidence for role of fadL gene product as long-chain fatty acid receptor.

J Biol Chem 1986, 261(1):167-171. PubMed   Abstract  | Publisher   Full   Text  

3. Wang X, Karbarz MJ, McGrath SC, Cotter RJ, Raetz CRH: MsbA Transporter-dependent Lipid A 1-

Dephosphorylation on the Periplasmic Surface of the Inner Membrane.

J Biol Chem 2004, 279(47):49470-49478. PubMed   Abstract  | Publisher   Full   Text  |

PubMed   Central   Full   Text  

The expression of full-length acyl-ACP thioesterase cDNA from Umbellularia californica in the E. coli fatty

acid-degradation mutant strain (fadD-) resulted in the secretion of free fatty acid into medium [9]. 

http://www.ncbi.nlm.nih.gov/pubmed/17901542

transport in

Modify fatty acids to be recognized to leave cell? Trick the cell?

Export to lipid bilayer

http://www.ncbi.nlm.nih.gov/pubmed/11278265

http://www.jbc.org/content/279/43/45102.long

Or do we want to engineer a method for phospholipid production for biofuel?