Contents Introduction - Bohemia Genetics

Dongsheng Biotech Co., Ltd. was established in 2005 in

Guangzhou China.

We focus on R&D and produce fundamental molecular

biology reagents. In the past 5 years, Dongsheng Biotech

has successfully grown up from a start-up to a professio-

nal organization. Our products have worldwide sales and

establish an excellent reputation.

We were ISO 9001:2008 certificated by BSI. We are also

authorized import and export manufacture in China. With

professional R&D group and strict control of the production

process, we now have customers all over the world.

Our mission is to provide professional and high quality

products for life science research. To make your research

more professional and more accurate, we are always

making our best efforts.

Introduction

Contents

DNA Laddes & Markers

For technical and sales inquiries:

Dongsheng Biotech Co., Ltd

Tel: +86-20-87791356, +86-20-28983724

Fax: +86-20-87791381

E-mail: [email protected]

Website: www.dongshengbio.com

Ordering Information

5

PCR, qPCR & RT-PCR 20

DNA Purification Kits 38

RNA Purification Kits 46

Silica Spin Column 50

1

2

3

4

Dongsheng Biotech Co., Ltd. was established in 2005 in

Guangzhou China.

We focus on R&D and produce fundamental molecular

biology reagents. In the past 5 years, Dongsheng Biotech

has successfully grown up from a start-up to a professio-

nal organization. Our products have worldwide sales and

establish an excellent reputation.

We were ISO 9001:2008 certificated by BSI. We are also

authorized import and export manufacture in China. With

professional R&D group and strict control of the production

process, we now have customers all over the world.

Our mission is to provide professional and high quality

products for life science research. To make your research

more professional and more accurate, we are always

making our best efforts.

Introduction

Contents

DNA Laddes & Markers

For technical and sales inquiries:

Dongsheng Biotech Co., Ltd

Tel: +86-20-87791356, +86-20-28983724

Fax: +86-20-87791381

E-mail: [email protected]

Website: www.dongshengbio.com

Ordering Information

5

PCR, qPCR & RT-PCR 20

DNA Purification Kits 38

RNA Purification Kits 46

Silica Spin Column 50

1

2

3

4

Key Products :

•

•

•

•

•

Taq DNA Polymerases

PCR Mix

DNA Ladders

PCR & Gel Extraction Kits

TRAzol

Product Line:

1. DNA Electrophoresis Products

2. PCR Products

3. DNA Purification Products

4, RNA Purification Products

5. Other Associative Reagents

.

Dongsheng Biotech provides wide range of molecular

biology products for life science research.

Additionally, all product provides custom synthesis.

Make Your Research

More Professional

More Accurate (1) Laboratory reagent kits producers, a smaller group of custo-

mers (<50 major customers worldwide) who purchase substa-

ntial quantities of nucleotide monomers as raw material for

specialized laboratory kits for use in research laboratories.

(2) Diagnostic kits producers who use our nucleotide monomers

as diagnostic agents in hospitals, blood collection agencies

and pathology laboratories primarily for amplification.

(3) Research laboratories in universities, industries, governments

and hospitals & medical schools.

Serve three major customer groups:

Dongsheng Biotech Product Application area

Key Products :

•

•

•

•

•

Taq DNA Polymerases

PCR Mix

DNA Ladders

PCR & Gel Extraction Kits

TRAzol

Product Line:

1. DNA Electrophoresis Products

2. PCR Products

3. DNA Purification Products

4, RNA Purification Products

5. Other Associative Reagents

.

Dongsheng Biotech provides wide range of molecular

biology products for life science research.

Additionally, all product provides custom synthesis.

Make Your Research

More Professional

More Accurate (1) Laboratory reagent kits producers, a smaller group of custo-

mers (<50 major customers worldwide) who purchase substa-

ntial quantities of nucleotide monomers as raw material for

specialized laboratory kits for use in research laboratories.

(2) Diagnostic kits producers who use our nucleotide monomers

as diagnostic agents in hospitals, blood collection agencies

and pathology laboratories primarily for amplification.

(3) Research laboratories in universities, industries, governments

and hospitals & medical schools.

Serve three major customer groups:

Dongsheng Biotech Product Application area

10bp ladder

20bp ladder

low ladder

50bp ladder

50bp ladder plus

100bp ladder

100 bp ladder plus

1kb ladder

1kb ladder plus

200 bp ladder

500bp ladder

?DNA/Hind

Marker 1

Marker 2

Marker 3

Marker 4

TMDS 2000

TMDS 5000

TMDS 10000

TMDS 15000

M1011

M1012

M1021

M1022

M1031

M1032

M1041

M1042

M1051

M1052

M1061

M1062

M1071

M1072

M1181

M1182

M1191

M1192

M1151

M1152

M1251

M1252

M1201

M1202

M1081

M1082

M1091

M1092

M1121

M1122

M1231

M1232

M1101

M1102

M1111

M1112

M1221

M1222

M1161

M1162

80-300bp

60-300bp

25-700bp

50-500bp

50-1,000bp

100-1,500bp

100-3,000bp

500-10,000bp

100-10,000bp

200-4,000bp

500-5,000bp

125-23,130bp

100-600bp

100-1,200bp

200-4,500bp

500-15,000bp

100-2,000bp

100-5,000bp

250-10,000bp

250-15,000bp

Product name Cat # Size Range

20x Swift DNA Electrophoresis Buffe

50xTAE Buffer

10xTBE

Gel Loading Dye(6x)

Gel Loading Dye, Blue(6x)

Gel Loading Dye, Three-colour (6x)

Gel Loading Dye, Orange(6x)

Gel Loading Dye, SDS (6x)

DSView Nucleic Acid Stain

Agarose

TM

M9011

M9021

M9031

M9041

M9051

M9061

M9071

M9081

M7011

N9051

500ml

500ml

500ml

5x1ml

5x1ml

5x1ml

5x1ml

5x1ml

1ml

500g

DNA Ladders & Markers Product List

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http://dongshengbio.com Tel: +86-20-877913565

1. DNA Ladders & Markers

E-mail: [email protected] Fax: +86-20-87791381 6

10bp ladder

20bp ladder

low ladder

50bp ladder

50bp ladder plus

100bp ladder

100 bp ladder plus

1kb ladder

1kb ladder plus

200 bp ladder

500bp ladder

?DNA/Hind

Marker 1

Marker 2

Marker 3

Marker 4

TMDS 2000

TMDS 5000

TMDS 10000

TMDS 15000

M1011

M1012

M1021

M1022

M1031

M1032

M1041

M1042

M1051

M1052

M1061

M1062

M1071

M1072

M1181

M1182

M1191

M1192

M1151

M1152

M1251

M1252

M1201

M1202

M1081

M1082

M1091

M1092

M1121

M1122

M1231

M1232

M1101

M1102

M1111

M1112

M1221

M1222

M1161

M1162

80-300bp

60-300bp

25-700bp

50-500bp

50-1,000bp

100-1,500bp

100-3,000bp

500-10,000bp

100-10,000bp

200-4,000bp

500-5,000bp

125-23,130bp

100-600bp

100-1,200bp

200-4,500bp

500-15,000bp

100-2,000bp

100-5,000bp

250-10,000bp

250-15,000bp

Product name Cat # Size Range

20x Swift DNA Electrophoresis Buffe

50xTAE Buffer

10xTBE

Gel Loading Dye(6x)

Gel Loading Dye, Blue(6x)

Gel Loading Dye, Three-colour (6x)

Gel Loading Dye, Orange(6x)

Gel Loading Dye, SDS (6x)

DSView Nucleic Acid Stain

Agarose

TM

M9011

M9021

M9031

M9041

M9051

M9061

M9071

M9081

M7011

N9051

500ml

500ml

500ml

5x1ml

5x1ml

5x1ml

5x1ml

5x1ml

1ml

500g

DNA Ladders & Markers Product List

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1. DNA Ladders & Markers


PCR and plasmid digestion by restriction enzymes are used for

the production of DNA fragments for our ladders/markers.

These products are stable during prolonged incubations at

Distinguishing features:

DNA Markers and Ladders

room temperature and multiple freeze-thaw cycles.

Storage

Bright, sharp bands for highly accurate sizing

Precise DNA concentration, quantitative

High resolution, low-background

Stable and uniform with different batches

Stable for 3 months at RT, 2 years -20℃.

Guideline for DNA Electrophoresis

Table 1. Different kinds of Agarose for different size of DNA range.

Agarose (%) Standard agarose High EEO agarose Low melt agarose Low melt low EEO agarose

Table 2. Different concentration of SDS-PAGE gel for different size of DNA range

SDS-PAGE gel (Polyacrylamide gel) is for separation of small size DNA range (≤1000bp)

SDS-PAGE gel (%)

3.55.

Effective DNA range

100 bp~1 kb80 bp~5

1. Electrophoresis gel

1.1 Agarose gel

1.2 SDS-PAGE gel


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2. Gel electrophoresis system

2.1 Electrophoresis buffer

Table 3.Guideline for electrophoresis buffer

Type

Swift DNAelecctrophoresis buffer

Feature

High speed

Low buffer capabilityHigh speed for ds linear DNATAE Buffer

TBE Buffer High buffer capabilityGood DNA separation, but low recovery rate

Application

High voltage and quick electrophoresisSmall amount but needs recovery DNA

Recommend for genomic DNAMacromolecular super helical DNADNA or RNA above 1500 bp

Recommend for DNA or RNA below 1500 bp

2.2 Electrophoresis condition

Table 4. Guideline for voltage and buffers for agarose gel electrophoresis

DNA size

＜1 kb

1~12 kb

＞12 kb

High throughput electrophoresis (~10kb)

Voltage

5~10 V/cm

4~10V/cm

1~3 V/cm

~23 V/cm

Buffer

TEB or Swift DNA electrophoresis buffer

TAE or TBE or Swift DNA electrophoresis buffer

TAE or Swift DNA electrophoresis buffer

Swift DNA electrophoresis buffer

DNA mobility in electrophoresis is effective by DNA size and structure, electrophoresis voltage, etc.

3. Guideline for DNA Ladders choice

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DNA Ladders & Markers

PCR and plasmid digestion by restriction enzymes are used for

the production of DNA fragments for our ladders/markers.

These products are stable during prolonged incubations at

Distinguishing features:

DNA Markers and Ladders

room temperature and multiple freeze-thaw cycles.

Storage

Bright, sharp bands for highly accurate sizing

Precise DNA concentration, quantitative

High resolution, low-background

Stable and uniform with different batches

Stable for 3 months at RT, 2 years -20℃.

Guideline for DNA Electrophoresis

Table 1. Different kinds of Agarose for different size of DNA range.

Agarose (%) Standard agarose High EEO agarose Low melt agarose Low melt low EEO agarose

Table 2. Different concentration of SDS-PAGE gel for different size of DNA range

SDS-PAGE gel (Polyacrylamide gel) is for separation of small size DNA range (≤1000bp)

SDS-PAGE gel (%)

3.55.

Effective DNA range

100 bp~1 kb80 bp~5

1. Electrophoresis gel

1.1 Agarose gel

1.2 SDS-PAGE gel


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2. Gel electrophoresis system

2.1 Electrophoresis buffer

Table 3.Guideline for electrophoresis buffer

Type

Swift DNAelecctrophoresis buffer

Feature

High speed

Low buffer capabilityHigh speed for ds linear DNATAE Buffer

TBE Buffer High buffer capabilityGood DNA separation, but low recovery rate

Application

High voltage and quick electrophoresisSmall amount but needs recovery DNA

Recommend for genomic DNAMacromolecular super helical DNADNA or RNA above 1500 bp

Recommend for DNA or RNA below 1500 bp

2.2 Electrophoresis condition

Table 4. Guideline for voltage and buffers for agarose gel electrophoresis

DNA size

＜1 kb

1~12 kb

＞12 kb

High throughput electrophoresis (~10kb)

Voltage

5~10 V/cm

4~10V/cm

1~3 V/cm

~23 V/cm

Buffer

TEB or Swift DNA electrophoresis buffer

TAE or TBE or Swift DNA electrophoresis buffer

TAE or Swift DNA electrophoresis buffer

Swift DNA electrophoresis buffer

DNA mobility in electrophoresis is effective by DNA size and structure, electrophoresis voltage, etc.

3. Guideline for DNA Ladders choice

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5 µl/lane, 8 cm gel0.5×TBE, 5 V/cm, 1h

─ 300 ? 280? 260

? 240? 220

? 200

? 180

? 160

? 140

? 120

? 100

? 60

? 80

40

40

40

100

40

40

40

40

100

40

40

40

40

10

% S

DS

-PA

GE

bp ng/5l

10bp ladder M1011/M1012

─ 300

bp ng/5l

? 200? 190

? 160

? 140

? 150

? 130

? 120

? 110

? 100

? 90

? 80

4040

100

40

40

100

40

40

40

40

40

40

─ 280 ─ 260 ─ 240

─ 220

? 180? 170

4040

40

404040

10

% S

DS

-PA

GE

10bp ladder is ideal for determining the size of double-stranded DNA from 80 to

300bp base pairs. The ladder consists of 18 linear double-stranded fragments.

The and p fragment are present at increased intensity to allow easy

identification. All fragments are precisely quantified and mixed during the

production. For 5 l loading, all fragments except 100bp and 200bp are 40ng. The

100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye

100bp 200b

Description

and is ready to use.

Contents(bp)

80, 90, , 110, 120, 130, 140, ,

220, 240,260, 280, 300

100 150, 160,170,180, 190, 200

Storage:

3 months at room temperature, 2 years at -20℃.

Concentration: 168ng/ l

Recommended Electrophoresis Condition

8cm 5% Agarose Gel, 0.5x TBE, 5V/cm, 1h

20cm 10% SDS-PAGE Gel, 1x TBE, 8V/cm, 3h

Recommended Loading : 2-5 l/lane

Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l

10 bp ladder Cat: M1011-50µg, M1012-5x50µg

20 bp ladder Cat. # M1021-50µg, M1022-5x50µg







100bp 200b

Description


Contents(bp)

60, 80, , 120, 140, 160, 180, , 220, 240,260, 280, 300100 200

Storage:









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The fragment is present at increased intensity to allow easy identification.

All fragments are precisely quantified and mixed during the production. For

5 l loading, all fragments except 250bp is 40ng. The 250bp is 100ng. This ladder

250bp

Description

is pre-mixed with loading dye and is ready to use.

Contents(bp)

50, 100, 200, , 300, 400,500150, 250

Storage:








50 bp ladder plus Cat. # M1051-50µg, M1052-5x50µg

50bp ladder plus is ideal for determining the size of double-stranded DNA from 50 to





250bp and 500bp fragment are 100ng. This ladder is pre-mixed wi th loading dye

250bp 500b

Description


Contents(bp)

50, 100, ,300, 400, , 600, 700,800, 900, 1000 150, 200, 250 500

Storage:







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5 µl/lane, 8 cm gel0.5×TBE, 5 V/cm, 1h

─ 300 ? 280? 260

? 240? 220

? 200

? 180

? 160

? 140

? 120

? 100

? 60

? 80

40

40

40

100

40

40

40

40

100

40

40

40

40

10

% S

DS

-PA

GE

bp ng/5l

10bp ladder M1011/M1012

─ 300

bp ng/5l

? 200? 190

? 160

? 140

? 150

? 130

? 120

? 110

? 100

? 90

? 80

4040

100

40

40

100

40

40

40

40

40

40

─ 280 ─ 260 ─ 240

─ 220

? 180? 170

4040

40

404040

10

% S

DS

-PA

GE







100bp 200b

Description


Contents(bp)

80, 90, , 110, 120, 130, 140, ,

220, 240,260, 280, 300

100 150, 160,170,180, 190, 200

Storage:









20 bp ladder Cat. # M1021-50µg, M1022-5x50µg







100bp 200b

Description


Contents(bp)

60, 80, , 120, 140, 160, 180, , 220, 240,260, 280, 300100 200

Storage:









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250bp

Description


Contents(bp)

50, 100, 200, , 300, 400,500150, 250

Storage:









50bp ladder plus is ideal for determining the size of double-stranded DNA from 50 to





250bp and 500bp fragment are 100ng. This ladder is pre-mixed wi th loading dye

250bp 500b

Description


Contents(bp)

50, 100, ,300, 400, , 600, 700,800, 900, 1000 150, 200, 250 500

Storage:







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500bp

Description


Contents(bp)

100, 300,400, , 600,700,800,900,1000,1500200, 500

Storage:




8cm 1.7% Agarose Gel, 0.5x TBE, 5V/cm, 1h




100bp ladder plus is ideal for determining the size of double-st randed DNA from 100 to

3000bp base pairs. The ladder consists of 14 linear double-st randed fragments.

The and fragment are present at increa sed intensi ty to allow easy

identif ication. Al l fragments are precisely quantif ied and mixed during the

product ion. For 5 l loading, all fragments except 500bp and 1200bp are 40ng. The


500bp 1200bp

Description


Contents(bp)

100, 200, ,600,700,800,900,1000, , 1500,2000,3000300, 400,500 1200

Storage:








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1kb ladder is ideal for determining the size of double-stranded DNA from 500 to


The and fragments are present at increased intensity to allow easy

identification. Al l fragments are precisely quantified and mixed during the production.

For 5 l loading, all fragments except 2000bp and 5000bp are 40ng. The 2000bp

and 5000bp are 100ng. Th is ladder

2000bp 5000bp

is pre-mixed wi th loading dye and is ready to use.

Description

Contents(bp)

500, 1000, , 3000, 4000, 1500, , 6000, 8000, 100002000 5000

Storage:




8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min



1 kb ladder Cat: M1181-50µg, M1182-5x50µg

1kb ladder plus is ideal for determining the size of double-stranded DNA from 100 to

10000bp base pa irs. The ladder consists of 15 linear double-stranded fragments.

The and p fragment are present at increased intensity to al low easy

ident ificat ion . Al l fragments are prec isely quantified and mixed during the

production. For 5 l loading, al l fragments except 500bp and 3000bp are 40ng. The

500bp and 3000bp fragment are 100ng. Thi s ladder is pre-mixed wi th loading dye

500bp 3000b

Description


Contents(bp)

100, ,700,1000,1500,2000, , 4000, 5000,6000, 8000, 10000 200, 300,400, 500 3000

Storage:





1kb ladder plus Cat. # M1191-50µg, M1192-5x50µg


Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l1

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500bp

Description


Contents(bp)

100, 300,400, , 600,700,800,900,1000,1500200, 500

Storage:








100bp ladder plus is ideal for determining the size of double-st randed DNA from 100 to

3000bp base pairs. The ladder consists of 14 linear double-st randed fragments.

The and fragment are present at increa sed intensi ty to allow easy

identif ication. Al l fragments are precisely quantif ied and mixed during the

product ion. For 5 l loading, all fragments except 500bp and 1200bp are 40ng. The


500bp 1200bp

Description


Contents(bp)

100, 200, ,600,700,800,900,1000, , 1500,2000,3000300, 400,500 1200

Storage:








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1kb ladder is ideal for determining the size of double-stranded DNA from 500 to


The and fragments are present at increased intensity to allow easy

identification. Al l fragments are precisely quantified and mixed during the production.

For 5 l loading, all fragments except 2000bp and 5000bp are 40ng. The 2000bp

and 5000bp are 100ng. Th is ladder

2000bp 5000bp

is pre-mixed wi th loading dye and is ready to use.

Description

Contents(bp)

500, 1000, , 3000, 4000, 1500, , 6000, 8000, 100002000 5000

Storage:







1 kb ladder Cat: M1181-50µg, M1182-5x50µg

1kb ladder plus is ideal for determining the size of double-stranded DNA from 100 to


The and p fragment are present at increased intensity to al low easy

ident ificat ion . Al l fragments are prec isely quantified and mixed during the

production. For 5 l loading, al l fragments except 500bp and 3000bp are 40ng. The

500bp and 3000bp fragment are 100ng. Thi s ladder is pre-mixed wi th loading dye

500bp 3000b

Description


Contents(bp)

100, ,700,1000,1500,2000, , 4000, 5000,6000, 8000, 10000 200, 300,400, 500 3000

Storage:





1kb ladder plus Cat. # M1191-50µg, M1192-5x50µg



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Low ladder is ideal for det ermining the size of doubl e-st randed DNA from 25bp to

700bp base pai rs. The ladder cons ists of 10 linear doubl e-st randed fragm ent s.

The and fragm ent s are presen t at incr ease d intensi ty to al low easy

ident ificat ion. Al l fragm ent s are preci sel y quant ified and mixed dur ing the produc tion.

For 5 l loadi ng, al l fragm ent s exc ept 100bp and 300bp are 40ng. The 100bp and

100bp 300bp

Description

Contents(bp)

25, , , 150,200, ,400,500,70050,75 100 300

Storage:







Low ladder Cat: M1031-50µg, M1032-5x50µg


4000bp base pa irs. The ladder consists of 12 linear double-stranded fragments. The

fragment is present at increased intensity to al low easy ident ificat ion. Al l

fragments are prec isely quantified and mixed during the production. For 5 l loading,

al l fragments except 1000bp is 40ng. The 1000bp fragment is 100ng. Thi s ladder is

1000bp

Description

pre-mixed with loading dye and is ready to use.

Contents(bp)

200, ,1200,1400,1600,1800,2000,2200,4000400, 600,800,1000

Storage:







200bp ladder Cat. # M1151-50µg, M1152-5x50µg

300bp are 100ng. This ladder is pre-mixed with loading dye and is ready to use.

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All fragments are precisely quantified and mixed during the production. For 5 l

loading, all fragments except 2000bp are 40ng. The 2000bp is 100ng. This ladder

2000bp

Description

Contents(bp)

500,1000, , 2500, 3000,3500,4000, 1500, 4500, 5000, 2000

Storage:







500bp ladder Cat: M1251-50µg, M1252-5x50µg

The Hind III digest of lambda DNA yields 8 fragments suitable for use as

molecular weight standards for agarose gel electrophoresis. Lambda

DNA/Hind III is pre-mixed with loading dye and is ready to use.

Description

Contents(bp)

125, 564, 2027,2322,4361,6557,9461,23130

Storage:



8cm 0.7% Agarose Gel, 1x TAE, 7V/cm, 45min

Lambda DNA/Hind III Cat. # M1201-50µg, M1202-5x50µg



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Low ladder is ideal for det ermining the size of doubl e-st randed DNA from 25bp to


The and fragm ent s are presen t at incr ease d intensi ty to al low easy

ident ificat ion. Al l fragm ent s are preci sel y quant ified and mixed dur ing the produc tion.

For 5 l loadi ng, al l fragm ent s exc ept 100bp and 300bp are 40ng. The 100bp and

100bp 300bp

Description

Contents(bp)

25, , , 150,200, ,400,500,70050,75 100 300

Storage:







Low ladder Cat: M1031-50µg, M1032-5x50µg


4000bp base pa irs. The ladder consists of 12 linear double-stranded fragments. The

fragment is present at increased intensity to al low easy ident ificat ion. Al l

fragments are prec isely quantified and mixed during the production. For 5 l loading,

al l fragments except 1000bp is 40ng. The 1000bp fragment is 100ng. Thi s ladder is

1000bp

Description

pre-mixed with loading dye and is ready to use.

Contents(bp)

200, ,1200,1400,1600,1800,2000,2200,4000400, 600,800,1000

Storage:







200bp ladder Cat. # M1151-50µg, M1152-5x50µg

300bp are 100ng. This ladder is pre-mixed with loading dye and is ready to use.

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All fragments are precisely quantified and mixed during the production. For 5 l

loading, all fragments except 2000bp are 40ng. The 2000bp is 100ng. This ladder

2000bp

Description

Contents(bp)

500,1000, , 2500, 3000,3500,4000, 1500, 4500, 5000, 2000

Storage:







500bp ladder Cat: M1251-50µg, M1252-5x50µg

The Hind III digest of lambda DNA yields 8 fragments suitable for use as

molecular weight standards for agarose gel electrophoresis. Lambda

DNA/Hind III is pre-mixed with loading dye and is ready to use.

Description

Contents(bp)

125, 564, 2027,2322,4361,6557,9461,23130

Storage:



8cm 0.7% Agarose Gel, 1x TAE, 7V/cm, 45min

Lambda DNA/Hind III Cat. # M1201-50µg, M1202-5x50µg



1

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3%

ag

aro

se

Mar ker 1 is ideal for determining the size of double-stranded DNA from 100 to

600bp base pairs. The ladder consists of 6 linear double-stranded fragmen ts.

The fragmen t is present at increased intensity to allow easy identification.

Al l fragmen ts are precisely quantified and mixed during the production. For

5 l loading, all fragmen ts except 400bp are 50ng. The 400bp is 125ng. Th is ladder

400bp

Description


Contents(bp)

100, 300, , 500, 600200, 400

Storage:




8cm 3% Agarose Gel, 0.5x TBE, 7V/cm, 40min



Marker 1 Cat: M1081-50µg, M1082-5x50µg

Marker 2 is ideal for determining the size of double-stranded DNA from 100 to


The fragment is present at increased intensity to al low easy ident ificat ion.

Al l fragments are prec isely quantified and mixed during the production. For

5 l loading, al l fragments except 700bp are 50ng. The 700bp is 125ng. Thi s ladder

700bp

Description


Contents(bp)

100, , 900,1200300, 500, 600, 700






Marker 2 Cat. # M1091-50µg, M1092-5x50µg

1.7

% a

ga

rose


1

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Ma

rke

rs

Storage:


1%

ag

aro

se





5 l loading, al l fragments except 1200bp are 75ng. The 1200bp is 150ng. Th is ladder

1200bp

Description

Contents(bp)

200, 500, , 2000, 3000,800, 45001200

Storage:








Marker 4 is ideal for det ermining the size of doubl e-st randed DNA from 500 to


The fragm ent is present at incr eased intensi ty to al low easy ident ificat ion.

Al l fragm ent s are preci sel y quant ified and mixed dur ing the product ion. For

5 l loadi ng, al l fragm ent s exc ept 2500bp are 50ng. The 2500bp is 125ng. Thi s ladder

2500bp

Description


Contents(bp)

500, , 5000, 8000,15000 1200, 2500






Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l1

% a

ga

rose


1

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ers



Storage:


3%

ag

aro

se

Mar ker 1 is ideal for determining the size of double-stranded DNA from 100 to





400bp

Description


Contents(bp)

100, 300, , 500, 600200, 400

Storage:




8cm 3% Agarose Gel, 0.5x TBE, 7V/cm, 40min









700bp

Description


Contents(bp)

100, , 900,1200300, 500, 600, 700







1.7

% a

ga

rose


1

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ad

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Ma

rke

rs

Storage:


1%

ag

aro

se





5 l loading, al l fragments except 1200bp are 75ng. The 1200bp is 150ng. Th is ladder

1200bp

Description

Contents(bp)

200, 500, , 2000, 3000,800, 45001200

Storage:








Marker 4 is ideal for det ermining the size of doubl e-st randed DNA from 500 to


The fragm ent is present at incr eased intensi ty to al low easy ident ificat ion.

Al l fragm ent s are preci sel y quant ified and mixed dur ing the product ion. For


2500bp

Description


Contents(bp)

500, , 5000, 8000,15000 1200, 2500







1%

ag

aro

se


1

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A L

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ers

& M

ark

ers



Storage:


DS 2000 is ideal for determining the size of double-stranded DNA from 100 to





750bp

Description


Contents(bp)

100, 500, , 1000, 2000200, 750

Storage:




8cm 1.7% Agarose Gel, 0.5x TBE, 7V/cm, 40min



DS 2000 Cat: M1101-50µg, M1102-5x50µg1

.7%

ag

aro

se

TM

TM






1000bp

Description


Contents(bp)

100, 250, 500, 750, , 1500, 2000, 3000, 50001000

Storage:







DS 5000 Cat: M1111-50µg, M1112-5x50µgTM

TM

1%

ag

aro

se


1

DN

A L

ad

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rs &

Ma

rke

rs

1%

ag

aro

se

1%

ag

aro

se

DS 10000 is ideal for det ermining the size of doubl e-st randed DNA from 250 to


The fragm ent is presen t at incr ease d intensi ty to al low easy ident ificat ion.

Al l fragm ent s are preci sel y quant ified and mixed dur ing the produc tion. For


2000bp

Description


Contents(bp)

250, 1000, , 4000,7000, 10000500, 2000

Storage:







DS 10000 Cat: M1221-50µg, M1222-5x50µgTM

TM






2500bp

Description


Contents(bp)

250, 1000, , 5000, 7500, 10000, 150002500

Storage:







DS 15000 Cat: M1161-50µg, M1162-5x50µgTM

TM

1

DN

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ad

ers

& M

ark

ers








750bp

Description


Contents(bp)

100, 500, , 1000, 2000200, 750

Storage:




8cm 1.7% Agarose Gel, 0.5x TBE, 7V/cm, 40min



DS 2000 Cat: M1101-50µg, M1102-5x50µg

1.7

% a

ga

rose

TM

TM






1000bp

Description


Contents(bp)

100, 250, 500, 750, , 1500, 2000, 3000, 50001000

Storage:







DS 5000 Cat: M1111-50µg, M1112-5x50µgTM

TM

1%

ag

aro

se


1

DN

A L

ad

de

rs &

Ma

rke

rs

1%

ag

aro

se

1%

ag

aro

se

DS 10000 is ideal for det ermining the size of doubl e-st randed DNA from 250 to


The fragm ent is presen t at incr ease d intensi ty to al low easy ident ificat ion.

Al l fragm ent s are preci sel y quant ified and mixed dur ing the produc tion. For


2000bp

Description


Contents(bp)

250, 1000, , 4000,7000, 10000500, 2000

Storage:







DS 10000 Cat: M1221-50µg, M1222-5x50µgTM

TM






2500bp

Description


Contents(bp)

250, 1000, , 5000, 7500, 10000, 150002500

Storage:







DS 15000 Cat: M1161-50µg, M1162-5x50µgTM

TM

1

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& M

ark

ers



TMDS is a collection of 30 highly-purified individual DNA fragmentsranging from 10-15,000 bp.

Custom DNA Ladders Service

We offer exceptionally pure DNA fragments that

are free of any truncated or degraded molecules.10 bp

15 bp

20 bp

25 bp

35 bp

50 bp

75 bp

100 bp

150 bp

200 bp

250 bp

300 bp

400 bp

500 bp

600 bp

700 bp

800 bp

900 bp

1000 bp

1200 bp

1500 bp

2000 bp

2500 bp

3000 bp

4000 bp

5000 bp

6000 bp

8000 bp

10000 bp

15000 bp

Storage Buffer (TE Buffer)

10 mM Tris-HCl (pH 7.6) with 1 mM EDTA

Quality Control

Tested on an Agilent 2100 bioanalyzer and by ap-

propriate gel electrophoresis. DNA concentration

is determined by spectrophotometer. The abse-

nce of nucleases confirmed by appropriate tests..

Storage

2 years at -20℃

Bulk orders can be customized to fit your needs:

Individual DNA fragments or mixtures.

Custom ladders of any combination and conce-

ntration.

Reference bands in a custom-made DNA ladder.

Flexible formulations.

Ready-to-use versions premixed with any load-

ing dye.

From microgram to gram quantities.

M8011

M8021

M8031

M8041

M8051

M8061

M8071

M8081

M8091

M8101

M8111

M8121

M8131

M8141

M8151

M8161

M8171

M8181

M8191

M8201

M8211

M8221

M8231

M8241

M8251

M8261

M8271

M8281

M8291

M8301

DNA Fragnent Size Cat # Amount

0.1

DNA Ladder/Marker Customized Service


1

DN

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Ma

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rs

目录目录目录目录

PCR相关产品1 PCR概述 ---------------------------------------------1.1 PCR原理及示意图 -----------------------------------------------

1.2 PCR实验注意事项 -----------------------------------------------

1.3 PCR产品选择指南 -----------------------------------------------

1.4 PCR产品简介 ----------------------------------------------------

2 嗜热DNA聚合酶 ------------------------------------Taq DNA 聚合酶 -------------------------------------------------------

TM HS Taq DNA 聚合酶 -------------------------------------------------

Pfu DNA 聚合酶 -------------------------------------------------------

Taq Plus DNA 聚合酶 -------------------------------------------------

Long Taq DNA 聚合酶 -------------------------------------------------TM FS Taq DNA 聚合酶 --------------------------------------------------

3 PCR Mix ---------------------------------------------PCR Mix ----------------------------------------------------------------

TM HS Taq Mix -----------------------------------------------------------

Pfu Mix ------------------------------------------------------------------

Plus Mix ----------------------------------------------------------------

Long Taq Mix ----------------------------------------------------------

SYBR Green qPCR Mix ---------------------------------------------- +Power SYBR Green PCR Master Mix（Rox ） ----------------------

TM FS Mix -----------------------------------------------------------TM FS Mix Direct for Blood ---------------------------------------TM FS Mix Direct for Tissue --------------------------------------

4 PCR Kit ----------------------------------------------PCR Kit -----------------------------------------------------------------

TM HS Kit -----------------------------------------------------------------

Pfu Kit ------------------------------------------------------------------

Long Taq Kit -----------------------------------------------------------TM FS Kit -----------------------------------------------------------------

5 PCR教学试剂盒 - - - - - - - - - - - - - - - - - - - - - - - - - - - -500 bp PCR反应试剂盒 ----------------------------------------------

1000 bp PCR反应试剂盒 --------------------------------------------

6 重组菌落PCR检测试剂盒 -------------------------通用菌落PCR检测试剂盒 --------------------------------------------

7 PCR辅助试剂 ---------------------------------------dNTPs ------------------------------------------------------------------

超纯水 ------------------------------------------------------------------

25 mM MgCl ----------------------------------------------------------2

PCR Enhancer --------------------------------------------------------

10×PCR Buffer --------------------------------------------------------

PCR标本处理液 -------------------------------------------------------

8 PCR常见问题分析 ---------------------------------

2626

26

29

31

3232

34

36

38

40

42

4444

46

48

50

52

54

56

58

60

62

6464

66

68

70

72

7474

74

7575

7676

76

76

77

77

78

79

2. PCR, qPCR & RT-PCR

TMDS is a collection of 30 highly-purified individual DNA fragmentsranging from 10-15,000 bp.

Custom DNA Ladders Service

We offer exceptionally pure DNA fragments that

are free of any truncated or degraded molecules.10 bp

15 bp

20 bp

25 bp

35 bp

50 bp

75 bp

100 bp

150 bp

200 bp

250 bp

300 bp

400 bp

500 bp

600 bp

700 bp

800 bp

900 bp

1000 bp

1200 bp

1500 bp

2000 bp

2500 bp

3000 bp

4000 bp

5000 bp

6000 bp

8000 bp

10000 bp

15000 bp

Storage Buffer (TE Buffer)

10 mM Tris-HCl (pH 7.6) with 1 mM EDTA

Quality Control

Tested on an Agilent 2100 bioanalyzer and by ap-

propriate gel electrophoresis. DNA concentration

is determined by spectrophotometer. The abse-

nce of nucleases confirmed by appropriate tests..

Storage

2 years at -20℃

Bulk orders can be customized to fit your needs:

Individual DNA fragments or mixtures.

Custom ladders of any combination and conce-

ntration.

Reference bands in a custom-made DNA ladder.

Flexible formulations.

Ready-to-use versions premixed with any load-

ing dye.

From microgram to gram quantities.

M8011

M8021

M8031

M8041

M8051

M8061

M8071

M8081

M8091

M8101

M8111

M8121

M8131

M8141

M8151

M8161

M8171

M8181

M8191

M8201

M8211

M8221

M8231

M8241

M8251

M8261

M8271

M8281

M8291

M8301

DNA Fragnent Size Cat # Amount

0.1

DNA Ladder/Marker Customized Service


1

DN

A L

ad

de

rs &

Ma

rke

rs

目录目录目录目录

PCR相关产品1 PCR概述 ---------------------------------------------1.1 PCR原理及示意图 -----------------------------------------------

1.2 PCR实验注意事项 -----------------------------------------------

1.3 PCR产品选择指南 -----------------------------------------------

1.4 PCR产品简介 ----------------------------------------------------

2 嗜热DNA聚合酶 ------------------------------------Taq DNA 聚合酶 -------------------------------------------------------

TM HS Taq DNA 聚合酶 -------------------------------------------------

Pfu DNA 聚合酶 -------------------------------------------------------

Taq Plus DNA 聚合酶 -------------------------------------------------

Long Taq DNA 聚合酶 -------------------------------------------------TM FS Taq DNA 聚合酶 --------------------------------------------------

3 PCR Mix ---------------------------------------------PCR Mix ----------------------------------------------------------------

TM HS Taq Mix -----------------------------------------------------------

Pfu Mix ------------------------------------------------------------------

Plus Mix ----------------------------------------------------------------

Long Taq Mix ----------------------------------------------------------

SYBR Green qPCR Mix ---------------------------------------------- +Power SYBR Green PCR Master Mix（Rox ） ----------------------

TM FS Mix -----------------------------------------------------------TM FS Mix Direct for Blood ---------------------------------------TM FS Mix Direct for Tissue --------------------------------------

4 PCR Kit ----------------------------------------------PCR Kit -----------------------------------------------------------------

TM HS Kit -----------------------------------------------------------------

Pfu Kit ------------------------------------------------------------------

Long Taq Kit -----------------------------------------------------------TM FS Kit -----------------------------------------------------------------

5 PCR教学试剂盒 - - - - - - - - - - - - - - - - - - - - - - - - - - - -500 bp PCR反应试剂盒 ----------------------------------------------

1000 bp PCR反应试剂盒 --------------------------------------------

6 重组菌落PCR检测试剂盒 -------------------------通用菌落PCR检测试剂盒 --------------------------------------------

7 PCR辅助试剂 ---------------------------------------dNTPs ------------------------------------------------------------------

超纯水 ------------------------------------------------------------------

25 mM MgCl ----------------------------------------------------------2

PCR Enhancer --------------------------------------------------------

10×PCR Buffer --------------------------------------------------------

PCR标本处理液 -------------------------------------------------------

8 PCR常见问题分析 ---------------------------------

2626

26

29

31

3232

34

36

38

40

42

4444

46

48

50

52

54

56

58

60

62

6464

66

68

70

72

7474

74

7575

7676

76

76

77

77

78

79

2. PCR, qPCR & RT-PCR


PCR (polymerase chain reaction) is a technique that is central to molec-

ular biology research. It is used in applications such as cloning, gene ex-

DNA amplification.

Dongsheng Biotech offers different series of products to help you achi-TM

eve PCR success. For enzymes, our Taq Polymerase, HS Taq DNATM

Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and

Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity

PCR performance. We also have Long Taq DNA Polymerase for long

We provi de four ser ies of PCR product s for di fferent uses:

PCR Polymerase Series

PCR Mix Series

PCR Kit Ser ies

qPCR Mix Series

RT-PCR Series

PC

R, q

PC

R &

RT

-PC

R

2

21

pression analysis, genotyping, sequencing, and mutant genes.

PCR Products

PCR, qPCR & RT-PCR


Propertie s of PCR enzymes

HS TaqTM

TMFS Taq

Enzyme

Taq

Pfu

Feature Application

General PCR/RT-PCR

DNA Mark/DNA Sequence

TA Clone

++

++

Fidelity

+ ++

PCR Product ends

3'-dA+++

+ ++++ 3'-dA

+++ + ++++ 3'-dA

5'→3' Activity

5'? 3' Activity

5'→3'Activity

5'→3' Activity

Fast-3 times of Taq

Amplification size

High fidelity PCR

Blunt end vector cloning

RT-PCR/cDNA clone and

express/SNP

+++++ +++

5'-3' Exonclease activity

Taq Plus

LongTaq

Fusion pfu High fidelity PCR

Long fragment PCR

++++

+++++++++

+++

++++++++

+++

++

++++

3'-dA/

Blunt end

GC rich template

Complicated template

High repeat sequencesHigh fidelity-4 times of Taq

Blunt end

General PCR/RT-PCR


TA Clone


5'? 3' Activity

High fidelity-4 times of Taq

5'→3' Activity

5'-3' Exonclease activity3'-dA/

Blunt end

GC rich template

Long template

Sequencing

Blunt end5'? 3' Activity


Sensivity

PC

R, q

PC

R &

RT

-PC

R

2

22

General PCR/RT-PCR


TA Clone

More and more convenient

Mo

re a

nd

mo

re re

find

fun

tion Cored by genetic engineering technology

Based by Taq DNA Polymerase


PCR (polymerase chain reaction) is a technique that is central to molec-

ular biology research. It is used in applications such as cloning, gene ex-

DNA amplification.

Dongsheng Biotech offers different series of products to help you achi-TM

eve PCR success. For enzymes, our Taq Polymerase, HS Taq DNATM

Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and

Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity

PCR performance. We also have Long Taq DNA Polymerase for long

We provi de four ser ies of PCR product s for di fferent uses:

PCR Polymerase Series

PCR Mix Series

PCR Kit Ser ies

qPCR Mix Series

RT-PCR Series

PC

R, q

PC

R &

RT

-PC

R

2

21

pression analysis, genotyping, sequencing, and mutant genes.

PCR Products

PCR, qPCR & RT-PCR


Propertie s of PCR enzymes

HS TaqTM

TMFS Taq

Enzyme

Taq

Pfu

Feature Application

General PCR/RT-PCR


TA Clone

++

++

Fidelity

+ ++

PCR Product ends

3'-dA+++

+ ++++ 3'-dA

+++ + ++++ 3'-dA

5'→3' Activity

5'? 3' Activity

5'→3'Activity

5'→3' Activity

Fast-3 times of Taq

Amplification size

High fidelity PCR

Blunt end vector cloning

RT-PCR/cDNA clone and

express/SNP

+++++ +++


Taq Plus

LongTaq

Fusion pfu High fidelity PCR

Long fragment PCR

++++

+++++++++

+++

++++++++

+++

++

++++

3'-dA/

Blunt end

GC rich template

Complicated template

High repeat sequencesHigh fidelity-4 times of Taq

Blunt end

General PCR/RT-PCR


TA Clone


5'? 3' Activity

High fidelity-4 times of Taq

5'→3' Activity

5'-3' Exonclease activity3'-dA/

Blunt end

GC rich template

Long template

Sequencing

Blunt end5'? 3' Activity


Sensivity

PC

R, q

PC

R &

RT

-PC

R

2

22

General PCR/RT-PCR


TA Clone

More and more convenient

Mo

re a

nd

mo

re re

find

fun

tion Cored by genetic engineering technology

Based by Taq DNA Polymerase


Product Name Cat # Size Description

Taq DNA Polymerase

TM HS Taq DNA Polymerase

Pfu DNA Polymerase

Taq Plus DNA Polymerase

Long Taq DNA Polymerase

TMFS Taq DNA Polymerase

P1011

P1012*

P1013

P1014*

P1015

P1081

P1082

P1083

P1084

P1021

P1022*

P1023

P1024*

P1031

P1032*

P1033

P1034*

P1061

P1062*

P1063

P1064*

P1071

P1072*

P1073

P1074*

500U

500U

1,000U

1,000U

18,000U

250U

500U

1,000U

18,000U

250U

250U

1,000U

1,000U

250U

250U

1,000U

1,000U

250U

250U

1,000U

1,000U

250U

250U

1,000U

1,000U

Robust Taq DNA Polymerase for routine

use incl.10x PCR Buffer, 6xloading buffer,

Mg2+ plus or free. Supply with 5U/ and

2.5U/ packages.

µl

µl

TM High specificity, incl. 10x HS PCR buffer,

6x loading buffer, Mg2+ plus or free.

Supply with 5U/ and 2.5U/ two

packages.

µl µl

High fidelity, incl.10x pfu buffer,

6xloading buffer, Mg2+ plus or free.


packages.

µl µl

Mix with Taq Polymerase and Pfu

Polymerase, long amplification and

high fidelity. Incl. 10xTaq Plus buffer,

Mg2+ plus or free. Supply with 5U/

and 2.5U/ two packages.

µl

µl

For long PCR, incl. 10xLong Taq buffer,


Supply with 5U/ and 2.5U two

packages.

µl /µl

TMFast, incl. 10xFS PCR buffer, 6xloading

buffer, Mg2+ plus or free. Supply with

5U/ and 2.5U/ two packages.µl µl

Taq Mix (2x)

TM HS Mix (2x)

Pfu Mix (2x)

P2011

P2011

P2081

P2082

P2021

P2022

1ml

5x1ml

1ml

5x1ml

1ml

5x1ml

Pre mix with Taq DNA Polymerase, dNTPs,

Mg2+ and Reaction buffer at optional conc-

entrations

TM Pre mix with HS Taq DNA Polymerase,

dNTPs, Mg2+ and Reaction buffer at optional

concentrations

. Pre mix with Pfu DNA Polymerase, dNTPs,


entrations.

PC

R, q

PC

R &

RT

-PR

2

23

TMFS Taq Mix Direct for Blood (2x)

TM FS Taq Mix Direct for Tissue (2x)

P3021

P3022

P3031

P3032

1ml

5x1ml

1ml

5x1ml

Pre mix with Long Taq DNA Polymerase, dNTPs,

Mg2+ and Reaction buffer at optional concentrations.

TM Pre mix with FS Taq DNA Polymerase, dNTPs, Mg2+

and Reaction buffer at optional concentrations.

TM Contains FS Taq Mix T(2x) and sample preparation

solution (Extraction solution and Neutralization solution

PCR, qPCR & RT-PCR

Long Mix (2x)P2061

P2062

1ml

5x1ml

TM FS Mix (2x)P3011

P3012

1ml

5x1m



Plus Mix (2x) P2031

P2031

1ml

5x1ml

Pre mix with Taq Plus DNA Polymerase, dNTPs,


PCR Products


dNTP Mix (2.5mM)

dNTP Mix (10mM)

dATP (100mM)

dTTP (100mM)

dCTP (100mM)

dGTP (100mM)

P9011

P9012

P9013

P9071

P9081

P9091

P9101

1ml

0.5ml

1ml

0.5ml

0.5ml

0.5ml

0.5ml

Contains 250U Taq DNA Polymerase and

5x1ml CR Reaction Mix (2x)

Contains 250U HS Taq DNA Polymerase

and 5x1ml HS Reaction Mix (2x)

Contains 250U FS Taq DNA Polymerase

and 5x1ml FS Reaction Mix (2x)

Contains 250U Long Taq DNA Polymerase

and 5x1ml Long Reaction Mix (2x)

With 15mM Mg2+

Without 15mM Mg2+

With Mg2+ concentration set

To prepare blood or tissue sample for

fast PCR

PC

R, q

PC

R &

RT

-PC

Rs

2

PCR, qPCR & RT-PCR

24

PCR Kit

TMHS Kit

Pfu Kit

Long Taq Kit

TMFS Kit

P3012

P3082

P3022

P3062

P3072

5x1ml

5x1ml

5x1ml

5x1ml

5x1ml

Contains 250U Pfu DNA Polymerase

and 5x1ml Pfu Reaction Mix (2x)

10xPCR buffer

MgCl (25mM)2

Nuclease-free Water

PCR Enhancer

PCR Sample Preparation Solution

P5011

P5011a

P5011b

P9031

P9021

P9022

P9023

P9041

P9051

P9052

4x1.25ml

4x1.25ml

6x1ml

4x1.25ml

5x1ml

100ml

500ml

500ul

50 preps

250 preps


RT-PCR Products


Mix reaction (2x) for real-time PCR

With low Rox+

With high Rox+

SYBR Green qPCR Mix

P2091

P2092

P2091a

P2092a

P2091b

P2092b

1ml

5x1ml

1ml

5x1ml

1ml

5x1ml

qPCR Products


®

®SYBR Green qPCR Mix (Rox )+

M-MLV (recombinant)

RT-PCR

RNasin (RNase inhibitor)

Oligo d(T) Primer15

Random Primer (6mer)

DEPC-treated Water

RNA Stabilization Solution

R1041

R1042

R1011

R1012

R2011

R2021

R2031

R2041

R2042

R2071

R2072

5000U

10000U

20 rxns

100 rxns

1000U

20µl

20µl

30ml

100ml

20ml

100ml



Taq DNA Polymerase

TM HS Taq DNA Polymerase

Pfu DNA Polymerase

Taq Plus DNA Polymerase

Long Taq DNA Polymerase


P1011

P1012*

P1013

P1014*

P1015

P1081

P1082

P1083

P1084

P1021

P1022*

P1023

P1024*

P1031

P1032*

P1033

P1034*

P1061

P1062*

P1063

P1064*

P1071

P1072*

P1073

P1074*

500U

500U

1,000U

1,000U

18,000U

250U

500U

1,000U

18,000U

250U

250U

1,000U

1,000U

250U

250U

1,000U

1,000U

250U

250U

1,000U

1,000U

250U

250U

1,000U

1,000U

Robust Taq DNA Polymerase for routine

use incl.10x PCR Buffer, 6xloading buffer,

Mg2+ plus or free. Supply with 5U/ and

2.5U/ packages.

µl

µl

TM High specificity, incl. 10x HS PCR buffer,

6x loading buffer, Mg2+ plus or free.


packages.

µl µl

High fidelity, incl.10x pfu buffer,



packages.

µl µl

Mix with Taq Polymerase and Pfu

Polymerase, long amplification and

high fidelity. Incl. 10xTaq Plus buffer,

Mg2+ plus or free. Supply with 5U/

and 2.5U/ two packages.

µl

µl

For long PCR, incl. 10xLong Taq buffer,


Supply with 5U/ and 2.5U two

packages.

µl /µl

TMFast, incl. 10xFS PCR buffer, 6xloading

buffer, Mg2+ plus or free. Supply with

5U/ and 2.5U/ two packages.µl µl

Taq Mix (2x)

TM HS Mix (2x)

Pfu Mix (2x)

P2011

P2011

P2081

P2082

P2021

P2022

1ml

5x1ml

1ml

5x1ml

1ml

5x1ml

Pre mix with Taq DNA Polymerase, dNTPs,


entrations

TM Pre mix with HS Taq DNA Polymerase,

dNTPs, Mg2+ and Reaction buffer at optional

concentrations

. Pre mix with Pfu DNA Polymerase, dNTPs,


entrations.

PC

R, q

PC

R &

RT

-PR

2

23

TMFS Taq Mix Direct for Blood (2x)

TM FS Taq Mix Direct for Tissue (2x)

P3021

P3022

P3031

P3032

1ml

5x1ml

1ml

5x1ml

Pre mix with Long Taq DNA Polymerase, dNTPs,




TM Contains FS Taq Mix T(2x) and sample preparation

solution (Extraction solution and Neutralization solution

PCR, qPCR & RT-PCR

Long Mix (2x)P2061

P2062

1ml

5x1ml

TM FS Mix (2x)P3011

P3012

1ml

5x1m



Plus Mix (2x) P2031

P2031

1ml

5x1ml

Pre mix with Taq Plus DNA Polymerase, dNTPs,


PCR Products


dNTP Mix (2.5mM)

dNTP Mix (10mM)

dATP (100mM)

dTTP (100mM)

dCTP (100mM)

dGTP (100mM)

P9011

P9012

P9013

P9071

P9081

P9091

P9101

1ml

0.5ml

1ml

0.5ml

0.5ml

0.5ml

0.5ml

Contains 250U Taq DNA Polymerase and

5x1ml CR Reaction Mix (2x)

Contains 250U HS Taq DNA Polymerase

and 5x1ml HS Reaction Mix (2x)

Contains 250U FS Taq DNA Polymerase

and 5x1ml FS Reaction Mix (2x)

Contains 250U Long Taq DNA Polymerase

and 5x1ml Long Reaction Mix (2x)

With 15mM Mg2+

Without 15mM Mg2+

With Mg2+ concentration set

To prepare blood or tissue sample for

fast PCR

PC

R, q

PC

R &

RT

-PC

Rs

2

PCR, qPCR & RT-PCR

24

PCR Kit

TMHS Kit

Pfu Kit

Long Taq Kit

TMFS Kit

P3012

P3082

P3022

P3062

P3072

5x1ml

5x1ml

5x1ml

5x1ml

5x1ml

Contains 250U Pfu DNA Polymerase

and 5x1ml Pfu Reaction Mix (2x)

10xPCR buffer

MgCl (25mM)2

Nuclease-free Water

PCR Enhancer


P5011

P5011a

P5011b

P9031

P9021

P9022

P9023

P9041

P9051

P9052

4x1.25ml

4x1.25ml

6x1ml

4x1.25ml

5x1ml

100ml

500ml

500ul

50 preps

250 preps


RT-PCR Products


Mix reaction (2x) for real-time PCR

With low Rox+

With high Rox+

SYBR Green qPCR Mix

P2091

P2092

P2091a

P2092a

P2091b

P2092b

1ml

5x1ml

1ml

5x1ml

1ml

5x1ml

qPCR Products


®

®SYBR Green qPCR Mix (Rox )+

M-MLV (recombinant)

RT-PCR

RNasin (RNase inhibitor)

Oligo d(T) Primer15

Random Primer (6mer)

DEPC-treated Water


R1041

R1042

R1011

R1012

R2011

R2021

R2031

R2041

R2042

R2071

R2072

5000U

10000U

20 rxns

100 rxns

1000U

20µl

20µl

30ml

100ml

20ml

100ml

Taq DNA Polymerase -routine Taq

Storage:

2 years at -20℃.

Code

P1011

P1012*

P1013

P1014*

P1015

Size

500U

500U

1000U

1000U

18,000U

* means supplied with dNTPs.

Description

Taq DNA Polymerase is a themostable recombinant DNA Polymerase derived

from Thermos aquaticus. It is a robust Taq for routine PCR use. Its molecular

weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5kb

(simple template). The elongation velocity is 0.9~1.2kb/min (70~75℃ ). It has

5' ? 3' exonuclease activity but lacks 3' ? 5’exonuclease activity that results

in a 3'-dA overhangs PCR product.

Description

Storage:

2 years at -20℃.

Contents:

Taq DNA Polymerase(5U/ l), 10xPCR Buffer, 6xLoading buffer,

dNTPs*(P1012, P1014)

Code

P1081

P1082

P1083

P1084

Size

250U

500U

1000U

18,000U

Contents:

, 10x PCR Buffer, 6xLoading bufferTM TM HS Taq DNA Polymerase(5U/ l) HS

TMHS Taq DNA Polymerase -high specificity

TM HS Taq DNA Polymer ase is a thermos table recombi nant DNA Polymer ase

derived from Thermos aquaticus. It contents the different reaction buffer that

is designed to provide highly specific ampl ification of nucleic acid templ ates

by PCR . The elongation velocity is 0.9~1.2kb/min .It is heat-stable

and wi ll synthesize DNA at elevated

(70~75 )

tempe ratures from single-stranded templ ates

Pfu DNA Polymerase -high fidelity

Storage:

2 years at -20℃.

Code

P1021

P1022*

P1023

P1024*

Size

250U

250U

1000U

1000U


Description

Pfu DNA Polymerase has superior thermostability and proofreading properties

compared to other thermostable polymerase. It can amplify DNA target up to

2kb (simple template). Its fidelity can reach 10 time of Taq DNA Polymerase.

The elongation velocity is 0.2~0.4kb/min (70~75℃ ). It results in blunt-ended

PCR products, which are ideal for cloning into blunt-ended vectors.Pru DNA

Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Contents:

Pfu DNA Polymerase(2.5U/ l), 10xPfu Buffer, 6xLoading buffer,

dNTPs* (P1022, P1024)

in the presence of a primer.


PC

R, q

PC

R &

RT

-PC

R

2

25

Taq Plus DNA Polymerase -high fidelity, high efficiency

Storage:

2 years at -20℃.

Code

P1031

P1032*

P1033

P1034*

Size

250U

250U

1000U

1000U


Description

Taq Plus DNA Polymerase is a mixture of Taq and Pfu polymerase, blends the

processivity of taq with the high fidelity of pfu. The two enzymes act synergis-

tically during PCR to generate more accurate, longer and greater yield PCR

products. It can amplify DNA target up to 20kb (simple template).

The error rate

Description

Storage:

2 years at -20℃.

Contents:

Taq Plus DNA Polymerase(2.5U/ l), 10xPCR Buffer, 6xLoading buffer,

dNTPs*(P1032, P1034)

Code

P1061

P1062*

P1063

P1064

Size

250U

250U

1000U

1000U

Contents:

, 10x Long PCR Buffer I, 10x Long PCR Buffer II

PCR Enhancer, 6xLoading buffer

Long Taq DNA Polymerase(5U/ l)

Long Taq DNA Polymerase -for complex templates

Long Taq DNA Polymerase, a combination of Taq and Pfu, is a special formulation

desigen for amplifying large fragment. This specially formulated Long Taq was

shown to amplify long templates from λ phage genome of up to 20 kb. It is also a

better choice for amplifying , such as . It will

complex templates GC-rich templates


Storage:

2 years at -20℃.

Code

P1071

P1072*

P1073

P1074*

Size

250U

250U

1000U

1000U


Description TM FS Taq DNA Polymerase, developed by Dongsheng Biotech, is the latst

generation Taq-based DNA Polymerase. It processes high amplification

efficiency as Taq polymerase does, and fast elongation ability as KOD

polymerase does,can be used as a variety of PCR. The elongation rate of TM FS Taq is 2 times higher than regular Taq. It will save the amplification time

Contents:

, 10x PCR Buffer, 6xLoading bufferTM TM FS Taq DNA Polymerase(5U/ l) FS

result in 3’-dA overhangs PCR products, which can be used In TA clone.

-fast Taq

-5is 7.5x10 per nucleotide per cycle.

by half.

PCR, qPCR & RT-PCR


PC

R, q

PC

R &

RT

-PC

R

2

26

Taq DNA Polymerase -routine Taq

Storage:

2 years at -20℃.

Code

P1011

P1012*

P1013

P1014*

P1015

Size

500U

500U

1000U

1000U

18,000U


Description

Taq DNA Polymerase is a themostable recombinant DNA Polymerase derived

from Thermos aquaticus. It is a robust Taq for routine PCR use. Its molecular

weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5kb

(simple template). The elongation velocity is 0.9~1.2kb/min (70~75℃ ). It has

5' ? 3' exonuclease activity but lacks 3' ? 5’exonuclease activity that results

in a 3'-dA overhangs PCR product.

Description

Storage:

2 years at -20℃.

Contents:

Taq DNA Polymerase(5U/ l), 10xPCR Buffer, 6xLoading buffer,

dNTPs*(P1012, P1014)

Code

P1081

P1082

P1083

P1084

Size

250U

500U

1000U

18,000U

Contents:

, 10x PCR Buffer, 6xLoading bufferTM TM HS Taq DNA Polymerase(5U/ l) HS

TMHS Taq DNA Polymerase -high specificity

TM HS Taq DNA Polymer ase is a thermos table recombi nant DNA Polymer ase

derived from Thermos aquaticus. It contents the different reaction buffer that

is designed to provide highly specific ampl ification of nucleic acid templ ates

by PCR . The elongation velocity is 0.9~1.2kb/min .It is heat-stable

and wi ll synthesize DNA at elevated

(70~75 )

tempe ratures from single-stranded templ ates

Pfu DNA Polymerase -high fidelity

Storage:

2 years at -20℃.

Code

P1021

P1022*

P1023

P1024*

Size

250U

250U

1000U

1000U


Description

Pfu DNA Polymerase has superior thermostability and proofreading properties

compared to other thermostable polymerase. It can amplify DNA target up to

2kb (simple template). Its fidelity can reach 10 time of Taq DNA Polymerase.

The elongation velocity is 0.2~0.4kb/min (70~75℃ ). It results in blunt-ended

PCR products, which are ideal for cloning into blunt-ended vectors.Pru DNA

Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Contents:

Pfu DNA Polymerase(2.5U/ l), 10xPfu Buffer, 6xLoading buffer,

dNTPs* (P1022, P1024)

in the presence of a primer.


PC

R, q

PC

R &

RT

-PC

R

2

25

Taq Plus DNA Polymerase -high fidelity, high efficiency

Storage:

2 years at -20℃.

Code

P1031

P1032*

P1033

P1034*

Size

250U

250U

1000U

1000U


Description

Taq Plus DNA Polymerase is a mixture of Taq and Pfu polymerase, blends the

processivity of taq with the high fidelity of pfu. The two enzymes act synergis-

tically during PCR to generate more accurate, longer and greater yield PCR

products. It can amplify DNA target up to 20kb (simple template).

The error rate

Description

Storage:

2 years at -20℃.

Contents:

Taq Plus DNA Polymerase(2.5U/ l), 10xPCR Buffer, 6xLoading buffer,

dNTPs*(P1032, P1034)

Code

P1061

P1062*

P1063

P1064

Size

250U

250U

1000U

1000U

Contents:

, 10x Long PCR Buffer I, 10x Long PCR Buffer II

PCR Enhancer, 6xLoading buffer

Long Taq DNA Polymerase(5U/ l)

Long Taq DNA Polymerase -for complex templates

Long Taq DNA Polymerase, a combination of Taq and Pfu, is a special formulation

desigen for amplifying large fragment. This specially formulated Long Taq was

shown to amplify long templates from λ phage genome of up to 20 kb. It is also a

better choice for amplifying , such as . It will

complex templates GC-rich templates


Storage:

2 years at -20℃.

Code

P1071

P1072*

P1073

P1074*

Size

250U

250U

1000U

1000U


Description TM FS Taq DNA Polymerase, developed by Dongsheng Biotech, is the latst

generation Taq-based DNA Polymerase. It processes high amplification

efficiency as Taq polymerase does, and fast elongation ability as KOD

polymerase does,can be used as a variety of PCR. The elongation rate of TM FS Taq is 2 times higher than regular Taq. It will save the amplification time

Contents:

, 10x PCR Buffer, 6xLoading bufferTM TM FS Taq DNA Polymerase(5U/ l) FS

result in 3’-dA overhangs PCR products, which can be used In TA clone.

-fast Taq

-5is 7.5x10 per nucleotide per cycle.

by half.

PCR, qPCR & RT-PCR


PC

R, q

PC

R &

RT

-PC

R

2

26

Taq Mix (2x) -routine PCR

Storage:

2 years at -20℃.

Code

P2011

P2012

Size

1ml

5x1ml

Description

Description

Storage:

2 years at -20℃.

Contents: 2xTaq Mix, Nuclease-free Water

Code

P2081

P2082

Size

1ml

5x1ml

Contents: 2x TMHS Mix, Nuclease-free Water

TMHS Mix (2x) -high specificity

Pfu Mix (2x) -high fidelity

Storage:

2 years at -20℃.

Code

P2021

P2022

Size

1ml

5x1ml

Description

Pfu Mix (2x) is a premixed, ready-to-use solution containing Pfu Taq DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for

efficient PCR amplification. To prepare the final PCR, only primers and template

DNA are added. Mix contributes to highly reproducible PCR by reducing the

risk of pipetting errors, miscalculation and contamination. It also contributes to

Pfu

Contents: 2x Pfu Mix, Nuclease-free Water

Taq Mix (2x) is a premixed, ready-to-use solution containing Taq DNA Polym-2+erase, dNTPs, Mg and Reaction Buffer at optional concentrations for efficient

PCR amplification. To prepare the final PCR, only primers and template DNA

are added. This pre-mixed formulation saves time and reduces contamination

due to the fewer pipetting steps required for PCR set up. The mix retains all

features of Taq DNA Polymerase.

TM TM HS HS

TM HS

Mix (2x) is a premixed, ready-to-use solution containing Taq DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for


DNA are added. Mix provides increased sensitivity, specificity, and yield,

while allowing assembly of reactions at RT. This mix contributes to highly rep-

roducible PCR by reducing the risk of pipetting errors, miscalculation and

contamination. It is convenient and also save time.

higher sensitivity by adding intersifier and optimizer. Pfu Taq is superior for tech-

nique that requires high fidelity DNA synthesis.


PC

R, q

PC

R &

RT

-PC

R

2

27

Plus Mix (2x) -high fidelity, high efficiency

Storage:

2 years at -20℃.

Code

P2031

P2032

Size

1ml

5x1ml

Description

Description

Storage:

2 years at -20℃.

Contents: 2xPlus Mix, Nuclease-free Water

Code

P2061

P2062

Size

1ml

5x1ml

Contents: 2xLong Taq Mix, Nuclease-free Water

Long Taq Mix (2x) -for complex templates

TMFS Mix (2x) -fast PCR

Storage:

2 years at -20℃.

Code

P2071

P2072

Size

1ml

5x1ml

Description

Contents: 2x Mix, Nuclease-free WaterTMFS

Plus Mix (2x) is a premixed, ready-to-use solution containing Taq Plus DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for


DNA are added. Plus Mis contributes to highly reproducible PCR by reducing

the risk of pipetting errors, miscalculation and contamination. It contains both

Long Taq Mix (2x) is a premixed, ready-to-use solution containing Long Taq DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for



the risk of pipetting errors, miscalculation and contamination. It also contributes

higher sensitivity by adding enhancer. It is good for complex templates, such as

GC-rich templates.

TM TM FS FS

The elongation rate of

.



DNA are added.This mix contributes to highly reproducible PCR by reducing the

risk of pipetting errors, miscalculation and contamination.

PCR, qPCR & RT-PCR


PC

R, q

PC

R &

RT

-PC

R

2

28

TM FS Taq is 2 times higher than regular Taq

Taq Mix (2x) -routine PCR

Storage:

2 years at -20℃.

Code

P2011

P2012

Size

1ml

5x1ml

Description

Description

Storage:

2 years at -20℃.

Contents: 2xTaq Mix, Nuclease-free Water

Code

P2081

P2082

Size

1ml

5x1ml

Contents: 2x TMHS Mix, Nuclease-free Water

TMHS Mix (2x) -high specificity

Pfu Mix (2x) -high fidelity

Storage:

2 years at -20℃.

Code

P2021

P2022

Size

1ml

5x1ml

Description

Pfu Mix (2x) is a premixed, ready-to-use solution containing Pfu Taq DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for


DNA are added. Mix contributes to highly reproducible PCR by reducing the

risk of pipetting errors, miscalculation and contamination. It also contributes to

Pfu

Contents: 2x Pfu Mix, Nuclease-free Water

Taq Mix (2x) is a premixed, ready-to-use solution containing Taq DNA Polym-2+erase, dNTPs, Mg and Reaction Buffer at optional concentrations for efficient

PCR amplification. To prepare the final PCR, only primers and template DNA

are added. This pre-mixed formulation saves time and reduces contamination

due to the fewer pipetting steps required for PCR set up. The mix retains all

features of Taq DNA Polymerase.

TM TM HS HS

TM HS



DNA are added. Mix provides increased sensitivity, specificity, and yield,

while allowing assembly of reactions at RT. This mix contributes to highly rep-

roducible PCR by reducing the risk of pipetting errors, miscalculation and

contamination. It is convenient and also save time.

higher sensitivity by adding intersifier and optimizer. Pfu Taq is superior for tech-

nique that requires high fidelity DNA synthesis.


PC

R, q

PC

R &

RT

-PC

R

2

27

Plus Mix (2x) -high fidelity, high efficiency

Storage:

2 years at -20℃.

Code

P2031

P2032

Size

1ml

5x1ml

Description

Description

Storage:

2 years at -20℃.

Contents: 2xPlus Mix, Nuclease-free Water

Code

P2061

P2062

Size

1ml

5x1ml

Contents: 2xLong Taq Mix, Nuclease-free Water

Long Taq Mix (2x) -for complex templates

TMFS Mix (2x) -fast PCR

Storage:

2 years at -20℃.

Code

P2071

P2072

Size

1ml

5x1ml

Description

Contents: 2x Mix, Nuclease-free WaterTMFS

Plus Mix (2x) is a premixed, ready-to-use solution containing Taq Plus DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for



the risk of pipetting errors, miscalculation and contamination. It contains both

Long Taq Mix (2x) is a premixed, ready-to-use solution containing Long Taq DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for



the risk of pipetting errors, miscalculation and contamination. It also contributes

higher sensitivity by adding enhancer. It is good for complex templates, such as

GC-rich templates.

TM TM FS FS

The elongation rate of

.



DNA are added.This mix contributes to highly reproducible PCR by reducing the

risk of pipetting errors, miscalculation and contamination.

PCR, qPCR & RT-PCR


PC

R, q

PC

R &

RT

-PC

R

2

28

TM FS Taq is 2 times higher than regular Taq

prepared sample

Code

P2071a

P2072a

Size

1ml

5x1ml

Description

TMFS Mix Direct for Blood (2x)

Storage:

2 years at -20℃.

Contents: 2x Mix B, Nuclease-free WaterTMFS

TMFSTM FS

Mix Direct for Blood (2x) is a premixed, ready-to-use solution

containing Taq DNA Polymerase, dNTPs, and all other PCR

components, except DNA template and primers. This mix is specific

for whole blood amplification. It contribute to fast, specific, sensitive

and reproducible PCR by reducing the fisk of contributes to highly

reproducible PCR by reducing the risk of pipetting errors, miscalc-

ulation and contamination. This product cut off the process of com-

TMFS Mix Direct for Tissue (2x)

Contents: 2x Mix T, Extraction Solution, Neutralization Solution

Nuclease-free Water

TMFS

whole or diluted blood

2x Mix B TMFS

primers water

plicated and waste time DNA extraction.

Code

P2071b

P2072b

Size

1ml

5x1ml

Description

Storage:

2 years at -20℃.

TMFSTM FS

Mix Direct for Tissue (2x) is a premixed, ready-to-use solution



for tissue direct amplification. It contribute to fast, specific, sensitive





Sample preparation

2x Mix T TMFS

primers water


PC

R, q

PC

R &

RT

-PC

R

2

29

PCR Kit -routine PCR

Storage:

2 years at -20℃.

Code

P3012

Size

5x1ml

Description

Description

Storage:

2 years at -20℃.

Code

P3082

Size

5x1ml

Contents: 2x TM TMHS Reaction Mix, HS Taq DNA Polymerase (2.5U/ l)

TMHS Kit -high specificity

Pfu Kit -high fidelity

Storage:

2 years at -20℃.

Code

P3022

Size

5x1ml

Description

Pfu Kit contains a Pfu DNA Polymerase, and a premixed, ready-to-use 2xPfu

Reaction Mix to perform 200 PCR reactions.

The PCR Kit contains a recombinant, high purified Taq DNA Polymerase, and

a premixed, ready-to-use 2xPCR Reaction Mix to perform 200 PCR reactions.

TM TM HS HS Kit contains a Taq DNA Polymerase, and a premixed, ready-to-use

2x Reaction Mix to perform 200 PCR reaction. TMHS

2+2xPfu Reaction Mix is a premixed solution containing dNTPs, Mg and Pfu

buffer at optional concentrations for efficient PCR amplification. PCR amplifi-

cation sensitivity is controllable because the amount of Pfu DNA Polymeras

Contents: 2xPCR Reaction Mix, Taq DNA Polymerase(2.5U/ l)

2+2xPCR Reaction Mix is a premixed solution containing dNTPs, Mg and PCR


cation sensitivity is controllable because the amount of Taq DNA Polymeras

is flexible.

2+2x Reaction Mix is a premixed solution containing dNTPs, Mg and

buffer at optional concentrations for efficient PCR amplification. PCR

amplification sensitivity is controllable because the amount of Taq DNA

TMHSTMHS

TM HS

Contents: 2xPfu Reaction Mix, Pfu DNA Polymerase(2.5U/ l)

PCR, qPCR & RT-PCR


PC

R, q

PC

R &

RT

-PC

R

2

30

Polymerase is flexible.

is flexible.

prepared sample

Code

P2071a

P2072a

Size

1ml

5x1ml

Description

TMFS Mix Direct for Blood (2x)

Storage:

2 years at -20℃.

Contents: 2x Mix B, Nuclease-free WaterTMFS

TMFSTM FS

Mix Direct for Blood (2x) is a premixed, ready-to-use solution



for whole blood amplification. It contribute to fast, specific, sensitive




TMFS Mix Direct for Tissue (2x)

Contents: 2x Mix T, Extraction Solution, Neutralization Solution

Nuclease-free Water

TMFS

whole or diluted blood

2x Mix B TMFS

primers water


Code

P2071b

P2072b

Size

1ml

5x1ml

Description

Storage:

2 years at -20℃.

TMFSTM FS

Mix Direct for Tissue (2x) is a premixed, ready-to-use solution



for tissue direct amplification. It contribute to fast, specific, sensitive





Sample preparation

2x Mix T TMFS

primers water


PC

R, q

PC

R &

RT

-PC

R

2

29

PCR Kit -routine PCR

Storage:

2 years at -20℃.

Code

P3012

Size

5x1ml

Description

Description

Storage:

2 years at -20℃.

Code

P3082

Size

5x1ml

Contents: 2x TM TMHS Reaction Mix, HS Taq DNA Polymerase (2.5U/ l)

TMHS Kit -high specificity

Pfu Kit -high fidelity

Storage:

2 years at -20℃.

Code

P3022

Size

5x1ml

Description

Pfu Kit contains a Pfu DNA Polymerase, and a premixed, ready-to-use 2xPfu

Reaction Mix to perform 200 PCR reactions.

The PCR Kit contains a recombinant, high purified Taq DNA Polymerase, and

a premixed, ready-to-use 2xPCR Reaction Mix to perform 200 PCR reactions.

TM TM HS HS Kit contains a Taq DNA Polymerase, and a premixed, ready-to-use

2x Reaction Mix to perform 200 PCR reaction. TMHS

2+2xPfu Reaction Mix is a premixed solution containing dNTPs, Mg and Pfu


cation sensitivity is controllable because the amount of Pfu DNA Polymeras

Contents: 2xPCR Reaction Mix, Taq DNA Polymerase(2.5U/ l)

2+2xPCR Reaction Mix is a premixed solution containing dNTPs, Mg and PCR


cation sensitivity is controllable because the amount of Taq DNA Polymeras

is flexible.




TMHSTMHS

TM HS

Contents: 2xPfu Reaction Mix, Pfu DNA Polymerase(2.5U/ l)

PCR, qPCR & RT-PCR


PC

R, q

PC

R &

RT

-PC

R

2

30


is flexible.

Storage:

2 years at -20℃.

Code

P3062

Size

5x1ml

Description

Description

Storage:

2 years at -20℃.

Code

P3072

Size

5x1ml

Contents: 2x TM TMFS Reaction Mix, FS Taq DNA Polymerase (2.5U/ l)

TMFS Kit -fast PCR

dNTPs

Storage:

2 years at -20℃.

Code

P9011

P9012

P9013

P9014

P9071

P9081

P9091

P9101

R2051

Size

1ml

0.5ml

1ml

4x0.25ml

0.5ml

0.5ml

0.5ml

0.5ml

0.5ml

Description

Long Taq Kit contains a Long Taq DNA Polymerase, PCR Enhancer, and a

premixed, ready-to-use 2xPCR Reaction Mix to perform 200 PCR reactions.

TM TM FS FS Kit contains a Taq DNA Polymerase, and a premixed, ready-to-use

2x Reaction Mix to perform 200 PCR reaction. TMFS

Contents: 2xLong Reaction Mix, PCR Enhancer, Taq DNA Polymerase(2.5U/ l)

2+2xLong Reaction Mix is a premixed solution containing dNTPs, Mg and Long

PCR buffer at optional concentrations for efficient PCR amplification. PCR

amplification sensitivity is controllable because the amount of Lont Taq DNA

.




TMFSTMFS

TM FS

Long Taq Kit -for complex templates

Product Name

dNTP Mix (2.5mM)

dNTP Mix (10mM)

dNTP Mix (10mM)

dNTP Set (100mM)

dATP (100mM)

dTTP (100mM)

dCTP (100mM)

dGTP (100mM)

dNTPs (10mM, RNase-free)

dNTP Mix is a aqueous solutions at PH 7.0

containing dATP, dTTP, dCTP, dGTP each

at a final concentration of 2.5mM (or 10mM).


PC

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PC

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Polymeras is flexible

dNTPs (10mM, RNase-free) is ideal for RT-

PCR reaction.

PCR Buffer

Storage:

2 years at -20℃.

Code

P5011

P5011a

P5011b

P9031

Size

4x1.25ml

4x1.25ml

6x1.25ml

4x1.25ml

Description Product Name2+10xPCR Buffer (with 15mM Mg )

2+10xPCR Buffer (without Mg )

10xPCR Buffer2+ (with Mg concentration set)

25mM MgCl2

10xPCR Buffer is recommended

for all PCR applications with Taq

DNA Polymerase (both recombinant

Nuclease-free Water

Storage:

2 years at -20℃.

Code

P9021

P9022

P9023

Size

5x1ml

100ml

500ml

Description Product Name

Nuclease-free WaterNuclease-free Water is deionized and

0.22 m membrane-filtered. It is suitable

for all molecular biology applications.

PCR Enhancer

Storage:

2 years at -20℃.

Code

P9041

Size

0.5ml


PCR EnhancerPCR Enhancer is used in conjunction with

DNA polymerase to optimized PCR of pro-

blemetic and/or GC-rich templates. The

system offers higher specificity, broader

magnesium concentration, broader anneal-

ing temperature and improved thermostable


Storage:

2 years at room temperature

Code

P9051

P9052

Size

50 pres

200 preps


PCR Sample Preparation SolutionPCR Sample Preparation Solution is widely

used for sample preparation before PCR

reaction. It can be used for samples like

tissue, hair, buccal swab, culture cells,

Contents: Extraction Solution

Neutralization Solution

PCR, qPCR & RT-PCR


PC

R, q

PC

R &

RT

-PC

R

2

32

serum, DNA virus.

DNA polymerase.

or native)

Storage:

2 years at -20℃.

Code

P3062

Size

5x1ml

Description

Description

Storage:

2 years at -20℃.

Code

P3072

Size

5x1ml

Contents: 2x TM TMFS Reaction Mix, FS Taq DNA Polymerase (2.5U/ l)

TMFS Kit -fast PCR

dNTPs

Storage:

2 years at -20℃.

Code

P9011

P9012

P9013

P9014

P9071

P9081

P9091

P9101

R2051

Size

1ml

0.5ml

1ml

4x0.25ml

0.5ml

0.5ml

0.5ml

0.5ml

0.5ml

Description

Long Taq Kit contains a Long Taq DNA Polymerase, PCR Enhancer, and a

premixed, ready-to-use 2xPCR Reaction Mix to perform 200 PCR reactions.

TM TM FS FS Kit contains a Taq DNA Polymerase, and a premixed, ready-to-use

2x Reaction Mix to perform 200 PCR reaction. TMFS

Contents: 2xLong Reaction Mix, PCR Enhancer, Taq DNA Polymerase(2.5U/ l)

2+2xLong Reaction Mix is a premixed solution containing dNTPs, Mg and Long

PCR buffer at optional concentrations for efficient PCR amplification. PCR

amplification sensitivity is controllable because the amount of Lont Taq DNA

.




TMFSTMFS

TM FS

Long Taq Kit -for complex templates

Product Name

dNTP Mix (2.5mM)

dNTP Mix (10mM)

dNTP Mix (10mM)

dNTP Set (100mM)

dATP (100mM)

dTTP (100mM)

dCTP (100mM)

dGTP (100mM)

dNTPs (10mM, RNase-free)

dNTP Mix is a aqueous solutions at PH 7.0

containing dATP, dTTP, dCTP, dGTP each

at a final concentration of 2.5mM (or 10mM).


PC

R, q

PC

R &

RT

-PC

R

2

31


Polymeras is flexible

dNTPs (10mM, RNase-free) is ideal for RT-

PCR reaction.

PCR Buffer

Storage:

2 years at -20℃.

Code

P5011

P5011a

P5011b

P9031

Size

4x1.25ml

4x1.25ml

6x1.25ml

4x1.25ml

Description Product Name2+10xPCR Buffer (with 15mM Mg )

2+10xPCR Buffer (without Mg )

10xPCR Buffer2+ (with Mg concentration set)

25mM MgCl2

10xPCR Buffer is recommended

for all PCR applications with Taq

DNA Polymerase (both recombinant

Nuclease-free Water

Storage:

2 years at -20℃.

Code

P9021

P9022

P9023

Size

5x1ml

100ml

500ml


Nuclease-free WaterNuclease-free Water is deionized and

0.22 m membrane-filtered. It is suitable

for all molecular biology applications.

PCR Enhancer

Storage:

2 years at -20℃.

Code

P9041

Size

0.5ml


PCR EnhancerPCR Enhancer is used in conjunction with

DNA polymerase to optimized PCR of pro-

blemetic and/or GC-rich templates. The

system offers higher specificity, broader

magnesium concentration, broader anneal-

ing temperature and improved thermostable


Storage:

2 years at room temperature

Code

P9051

P9052

Size

50 pres

200 preps


PCR Sample Preparation SolutionPCR Sample Preparation Solution is widely

used for sample preparation before PCR

reaction. It can be used for samples like

tissue, hair, buccal swab, culture cells,

Contents: Extraction Solution


PCR, qPCR & RT-PCR


PC

R, q

PC

R &

RT

-PC

R

2

32

serum, DNA virus.

DNA polymerase.

or native)

Real Time qPCR Master Mix

Keep Features:

·High Specificity

·High Sensitivity

·Universal

eliminate non-specific and formation of primer dimers.

detects low copy number targets.

suitable for most real-time thermal cyclers.

Plasmid DNA 10ng, 1ng, 0.1ng, 0.01ng, 0.001, 0.0001ng/ul in BIO-RAD CFX96

DongshengDongsheng

Plasmid DNA 1ng, 0.1ng, 0.01ng, 0.001ng/ul in BIO-RAD CFX96

Company ACompany A

SYBR Green qPCR Mix

SYBR Green qPCR Mix (High Rox+)

SYBR Green qPCR Mix (Low Rox+)

Probe qPCR Mix

Probe qPCR Mix (High Rox+)

Probe qPCR Mix (Low Rox+)

Product

Machine

Instrument guide table

Rox Reference Dye


PC

R, q

PC

R &

RT

-PC

R

2

33

SYBR Green qPCR Mix -realtime PCR

Storage:

Protect from light, 2 years at -20℃.

Code

P2091

P2092

Size

1ml

5x1ml

Description

Contents: 2xSYBR Green qPCR Mix , Nuclease-free Water

SYBR Green qPCR Mix (2x) is designed for high-performance, high-through-

put, real-time PCR. The kit contains a Hot Start Taq DNA Polymerase engineered

a process of molecular evolution. This results in a unique enzyme, specifically

designed for qPCR using SYBR Green I dye chemistry.

SYBR Green qPCR Mix (2x) is a convenient premix of the components (except

primers, template, and water) necessary to perform real-time polymerase chain

reaction (PCR) using SYBR Green I dye with enhanced sensitivity and specificity.

The SYBR Green dye binds to double-strand (ds) DNA, thus providing a fluore-

sent signal that reflects the amount of dsDNA product generated during PCR.

Amplification Melt Curve

Above are with Dongsheng’s SYBR Green qPCR Mix in STRATAGENE Mx3005.

Sample: HBV Serum, Objective band: 210bp.

Ampification condition: 95C 20s in the first cycle, 95C 10s, 55C 20s and 72C 30s in the less 40 cycles.

Recommended PCR

Reaction ( 20µl

reaction system)

Components

2xSYBR Green qPCR Mix

Forward primer (10µM)

Reverse primer (10 µM)

Template DNA

Nuclease-free, Water

Volume

10 µl

0.4 µl

0.4 µl

variable

to 20 µl

Final concentration

1X

0.2 µM

0.2 µM

<100ng

-

Recommend PCR

Cycling System

Initial Denaturation

Denature

Anneal

Extend*

Melting Curve Analysis

94℃

95℃

60℃

72℃

3 min

15 sec

15 sec

20 sec

40 cycles

*Dongsheng cat #P9021/P9022 work better for 3 step cycling protocol. We don’t

recommend to use 2 step cycling protocol with this product.

PCR, qPCR & RT-PCR


PC

R, q

PC

R &

RT

-PC

R

2

34

®

®

®

®

®

®

®

®

Real Time qPCR Master Mix

Keep Features:

·High Specificity

·High Sensitivity

·Universal

eliminate non-specific and formation of primer dimers.

detects low copy number targets.

suitable for most real-time thermal cyclers.

Plasmid DNA 10ng, 1ng, 0.1ng, 0.01ng, 0.001, 0.0001ng/ul in BIO-RAD CFX96

DongshengDongsheng

Plasmid DNA 1ng, 0.1ng, 0.01ng, 0.001ng/ul in BIO-RAD CFX96

Company ACompany A

SYBR Green qPCR Mix

SYBR Green qPCR Mix (High Rox+)

SYBR Green qPCR Mix (Low Rox+)

Probe qPCR Mix

Probe qPCR Mix (High Rox+)

Probe qPCR Mix (Low Rox+)

Product

Machine

Instrument guide table

Rox Reference Dye


PC

R, q

PC

R &

RT

-PC

R

2

33

SYBR Green qPCR Mix -realtime PCR

Storage:


Code

P2091

P2092

Size

1ml

5x1ml

Description

Contents: 2xSYBR Green qPCR Mix , Nuclease-free Water

SYBR Green qPCR Mix (2x) is designed for high-performance, high-through-

put, real-time PCR. The kit contains a Hot Start Taq DNA Polymerase engineered

a process of molecular evolution. This results in a unique enzyme, specifically

designed for qPCR using SYBR Green I dye chemistry.

SYBR Green qPCR Mix (2x) is a convenient premix of the components (except

primers, template, and water) necessary to perform real-time polymerase chain

reaction (PCR) using SYBR Green I dye with enhanced sensitivity and specificity.

The SYBR Green dye binds to double-strand (ds) DNA, thus providing a fluore-

sent signal that reflects the amount of dsDNA product generated during PCR.

Amplification Melt Curve

Above are with Dongsheng’s SYBR Green qPCR Mix in STRATAGENE Mx3005.

Sample: HBV Serum, Objective band: 210bp.

Ampification condition: 95C 20s in the first cycle, 95C 10s, 55C 20s and 72C 30s in the less 40 cycles.

Recommended PCR

Reaction ( 20µl

reaction system)

Components

2xSYBR Green qPCR Mix



Template DNA


Volume

10 µl

0.4 µl

0.4 µl

variable

to 20 µl

Final concentration

1X

0.2 µM

0.2 µM

<100ng

-

Recommend PCR

Cycling System


Denature

Anneal

Extend*


94℃

95℃

60℃

72℃

3 min

15 sec

15 sec

20 sec

40 cycles

*Dongsheng cat #P9021/P9022 work better for 3 step cycling protocol. We don’t

recommend to use 2 step cycling protocol with this product.

PCR, qPCR & RT-PCR


PC

R, q

PC

R &

RT

-PC

R

2

34

®

®

®

®

®

®

®

®

Melt CurveAmplification

Above are with Dongsheng’s SYBR Green qPCR Mix (High Rox+) in STRATAGENE Mx3005

+SYBR Green qPCR Mix (Rox ) -high accurate real-time PCR

Storage:


Code

P2091a

P2092a

P2091b

P2092b

Size

1ml

5x1ml

1ml

5x1ml

Description

Contents:

#P2091a/P2092a: 2xSYBR Green qPCR Mix (Low Rox ) , Nuclease-free Water+

SYBR Green qPCR Mix (Rox ) (2x) is designed for high-performance, high-

throughput, real-time PCR. The kit contains a Hot Start Taq DNA Polymerase

engineered a process of molecular evolution. This results in a unique enzyme,

specifically designed for qPCR using SYBR Green I dye chemistry. It also

contains rox reference dye, which is ideal for using in amplification and detec-

tion of DNA in qPCR on real-time instruments (e.g. ABI) that supports normali-

zation with rox reference dye .

+

Recommended PCR

Reaction ( 20µl

reaction system)

Components

2xSYBR Green qPCR Mix (Rox )



Template DNA


+

Volume

10 µl

0.4 µl

0.4 µl

variable

to 20 µl

Final concentration

1X

0.2 µM

0.2 µM

<100ng

-

Recommend PCR

Cycling System


Denature

Anneal

Extend*


94℃

95℃

60℃

72℃

3 min

15 sec

15 sec

20 sec

40 cycles

*Dongsheng cat #P9021a/P9022a/P9021b/P9022b work better for 3 step cycling

protocol. We don’t recommend to use 2 step cycling protocol with this product.

#P2091b/P2092b: 2xSYBR Green qPCR Mix (High Rox ) , Nuclease-free Water+


PC

R, q

PC

R &

RT

-PC

R

2

35

®

®

®

®

®

®

M-MLV (recombinant) Cat.# R1041-5000U, R1042-10000U

Storage:

2 years at -20℃.

R1041

25 µl

100 µl

R1042

50 µl

200 µl

Description

Contents

M-MLV (200U/ µl)

5xfirst-strand buffer (5x)

Moloney Murine Leukemia Virus Reverse Trans-

criptase (M-MLV RT) uses single-stranded RNA

or DNA in the presence of a primer to synthesize

a complementary DNA strand. This enzyme is

isolated from E. coli expressing a portion of the

pol gene of M-MLV on a plasmid. The enzyme is

used to synthesize first-strand cDNA up to 5kb.

RT-PCR Kit Cat.# R1011-20 rxns, R1012-100 rxns

Storage:

2 years at -20℃.

R1011

20µl

12µl

20µl

20µl

80µl

1ml

50µl

R1011

100µl

60µl

100µl

100µl

400µl

5x1ml

250µl

Description

Contents

M-MLV (200U/ µl)

RNasin (40U/µl)

(50µM)

Random primer (50µM)


RNase-free ddH2O

dNTPs (10mM each)

Oligo d(T) 15

RT-PCR Kit is optimized to synthesize first-strand

cDNA from purif ied poly (A)+ or total RNA.

The kit Reverse Transcriptase is a version of M-MLV

RT that has been engineered to reduce RNase H

activity and provide increased thermal stability. The

enzyme is used to synthsize cDNA at a temperature

range of 42-55℃ , providing increased specificity,

higher yields of cDNA, and more full-length product

R1041can be used for 25 reactions, R1042 can

be used for 50 reactions (20 µl PCR system).

Rnasin (RNase inhibitor) Cat.# R2011-1000U

Storage:

2 years at -20℃.

R2011

25µl

Description

Contents

RNasin (40U/µl)

Rnase is a ribonuclease inhibitor purified form

human placenta. RNase recuces the activity of

RNase A-type enzymes in a variety of organisms.

It is free of endodeoxyribonucleases, exodeoxy-

ribonucleases, ribonucleases and phosphatases.

PCR, qPCR & RT-PCR


PC

R, q

PC

R &

RT

-PC

R

2

36

Reverse Transcriptase

than other reverse transcriptased.

Melt CurveAmplification

Above are with Dongsheng’s SYBR Green qPCR Mix (High Rox+) in STRATAGENE Mx3005

+SYBR Green qPCR Mix (Rox ) -high accurate real-time PCR

Storage:


Code

P2091a

P2092a

P2091b

P2092b

Size

1ml

5x1ml

1ml

5x1ml

Description

Contents:

#P2091a/P2092a: 2xSYBR Green qPCR Mix (Low Rox ) , Nuclease-free Water+

SYBR Green qPCR Mix (Rox ) (2x) is designed for high-performance, high-

throughput, real-time PCR. The kit contains a Hot Start Taq DNA Polymerase

engineered a process of molecular evolution. This results in a unique enzyme,

specifically designed for qPCR using SYBR Green I dye chemistry. It also

contains rox reference dye, which is ideal for using in amplification and detec-

tion of DNA in qPCR on real-time instruments (e.g. ABI) that supports normali-

zation with rox reference dye .

+

Recommended PCR

Reaction ( 20µl

reaction system)

Components

2xSYBR Green qPCR Mix (Rox )



Template DNA


+

Volume

10 µl

0.4 µl

0.4 µl

variable

to 20 µl

Final concentration

1X

0.2 µM

0.2 µM

<100ng

-

Recommend PCR

Cycling System


Denature

Anneal

Extend*


94℃

95℃

60℃

72℃

3 min

15 sec

15 sec

20 sec

40 cycles

*Dongsheng cat #P9021a/P9022a/P9021b/P9022b work better for 3 step cycling

protocol. We don’t recommend to use 2 step cycling protocol with this product.

#P2091b/P2092b: 2xSYBR Green qPCR Mix (High Rox ) , Nuclease-free Water+


PC

R, q

PC

R &

RT

-PC

R

2

35

®

®

®

®

®

®

M-MLV (recombinant) Cat.# R1041-5000U, R1042-10000U

Storage:

2 years at -20℃.

R1041

25 µl

100 µl

R1042

50 µl

200 µl

Description

Contents

M-MLV (200U/ µl)


Moloney Murine Leukemia Virus Reverse Trans-

criptase (M-MLV RT) uses single-stranded RNA

or DNA in the presence of a primer to synthesize

a complementary DNA strand. This enzyme is

isolated from E. coli expressing a portion of the

pol gene of M-MLV on a plasmid. The enzyme is

used to synthesize first-strand cDNA up to 5kb.

RT-PCR Kit Cat.# R1011-20 rxns, R1012-100 rxns

Storage:

2 years at -20℃.

R1011

20µl

12µl

20µl

20µl

80µl

1ml

50µl

R1011

100µl

60µl

100µl

100µl

400µl

5x1ml

250µl

Description

Contents

M-MLV (200U/ µl)

RNasin (40U/µl)

(50µM)

Random primer (50µM)


RNase-free ddH2O

dNTPs (10mM each)

Oligo d(T) 15

RT-PCR Kit is optimized to synthesize first-strand

cDNA from purif ied poly (A)+ or total RNA.

The kit Reverse Transcriptase is a version of M-MLV

RT that has been engineered to reduce RNase H

activity and provide increased thermal stability. The

enzyme is used to synthsize cDNA at a temperature

range of 42-55℃ , providing increased specificity,

higher yields of cDNA, and more full-length product

R1041can be used for 25 reactions, R1042 can

be used for 50 reactions (20 µl PCR system).

Rnasin (RNase inhibitor) Cat.# R2011-1000U

Storage:

2 years at -20℃.

R2011

25µl

Description

Contents

RNasin (40U/µl)

Rnase is a ribonuclease inhibitor purified form

human placenta. RNase recuces the activity of

RNase A-type enzymes in a variety of organisms.

It is free of endodeoxyribonucleases, exodeoxy-

ribonucleases, ribonucleases and phosphatases.

PCR, qPCR & RT-PCR


PC

R, q

PC

R &

RT

-PC

R

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36

Reverse Transcriptase

than other reverse transcriptased.

Oligo d(T) Primer Cat.# R2021-20µl 15

Storage:

2 years at -20℃.

R2021

20 µl

Description

Contents

(50µM)Oligo d(T) Primer 15

Oligo d(T) Primer is a synthetic single-stranded 15-15

mer oligobucleotide with 5’-and 3’-hydroxyl ends. This

primer is supplied as an aqueous solution and can be

Random Primer Cat.# R2031-20µl (6mer)

DEPC-treated Water Cat.# R2041-30ml, R2042-100ml

Storage:

2 years at 4℃.

R2041

30ml

Description

Contents

DEPC-treated Water

DEPC-treated Water is deionized, diethylpyrocarbonate

(DEPC) treated and 0.22 µM membrane-filtered. It is

recomme nded to be used in any application where RNA

Storage:

2 years at -20℃.

R2031

20 µl

Description

Contents

(50µM)Random Primer (6mer)

Random Primer is a mixture of single-stranded 6-(6mer)

mer random hexanucleotides with 5’-and 3’-hydroxyl

ends. This primer is supplied as an aqueous solution

R2042

100ml

RNA Stabilization Solution Cat.# R2071-20ml, R2072-100ml

Storage:

2 years at room temperature.

R2071

20ml

Description

Contents


RNA Stabi lization Sol ut ion is an aqu eou s tissue storage

reag ent that stabi lize and protects ce llular RNA in intact,

unf rozen tissue samples. It el iminat es the nee d to imm-

edi ately process tissue samples or to freez e samples in

R2072

100ml

used for first strand cDNA synthesis.

and can be used for first strand cDNA synthesis.

is involed.

liquid nitrogen for later processing.


PC

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PC

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RT

-PC

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2

375

2

3. DNA Purification Kits

Oligo d(T) Primer Cat.# R2021-20µl 15

Storage:

2 years at -20℃.

R2021

20 µl

Description

Contents

(50µM)Oligo d(T) Primer 15

Oligo d(T) Primer is a synthetic single-stranded 15-15

mer oligobucleotide with 5’-and 3’-hydroxyl ends. This

primer is supplied as an aqueous solution and can be

Random Primer Cat.# R2031-20µl (6mer)

DEPC-treated Water Cat.# R2041-30ml, R2042-100ml

Storage:

2 years at 4℃.

R2041

30ml

Description

Contents

DEPC-treated Water

DEPC-treated Water is deionized, diethylpyrocarbonate

(DEPC) treated and 0.22 µM membrane-filtered. It is

recomme nded to be used in any application where RNA

Storage:

2 years at -20℃.

R2031

20 µl

Description

Contents

(50µM)Random Primer (6mer)

Random Primer is a mixture of single-stranded 6-(6mer)

mer random hexanucleotides with 5’-and 3’-hydroxyl

ends. This primer is supplied as an aqueous solution

R2042

100ml

RNA Stabilization Solution Cat.# R2071-20ml, R2072-100ml

Storage:

2 years at room temperature.

R2071

20ml

Description

Contents


RNA Stabi lization Sol ut ion is an aqu eou s tissue storage

reag ent that stabi lize and protects ce llular RNA in intact,

unf rozen tissue samples. It el iminat es the nee d to imm-

edi ately process tissue samples or to freez e samples in

R2072

100ml

used for first strand cDNA synthesis.

and can be used for first strand cDNA synthesis.

is involed.

liquid nitrogen for later processing.


PC

R, q

PC

R &

RT

-PC

R

2

375

2

3. DNA Purification Kits


DNA Purification Kits

Dongsheng Biotech offers a broad range of kits for the isolation and

purification of genomic and plasmid DNA from a wide variety of samples,

including tissue, cells , blood, serum, bacterial, yeast, and plants samples .

We provide three series for different materials purification:'

Plasmid DNA Extraction Kits

DNA Product Purification Kits

Genimic DNA Purification Kits

DN

A P

uri

fic

ati

on

Kit

s

3

39



Guides of DNA Purification Kits

Quick Genomic DNA Extraction

Kit

Standard

Agarose Gel Safe, Healthy

Gel Extraction Kit

Eco-Friendly Gel Extraction Kit

PCR Product/

DNA Fragment

SolutionSafe, Healthy

PCR and DNA Fragment

Purification Kit

Eco-Friendly PCR and DNA

Fragment Purification Kit

DNA Product

Purification

Genomic DNA

Purification

Insect/ Animal

Nematoda

Sample size: less than

10mg tissue

Blood

Sample size: 0.l-1ml

flesh blood/ frozen blood

Blood Genomic DNA

Extraction Kit


200ul whole bloodQuick Blood Genomic DNA

Extraction Kit

Tissue/

Cell

Bacteria

Yeast

Sample size: less than6 710mg tissue/ 10 -10

culture cell

Sample size: 0.5-2ml/6less than 10 -10 bacteria

Sample size: less than71-5x10 Yeast

Quick Tissue/Culture Cells

Genomic DNA Extraction Kit

Quick Bacteria Genomic

DNA Extraction Kit

Quick Yeast Genomic DNA

Extraction Kit

Application Material Experimental requirement Product Choose

Plasmid DNA

ExtractionBacteria

Sample size:1-5ml

Sample size:1-5ml Safe, Healthy

High Purity Plasmid Miniprep Kit

Sample size:100-200ml

Simple, Convenient

Eco-Friendly Plasmid Miniprep Kit

Plasmid DNA Maxiprep Kit (High

Purifity, Silica-membrant Spin

Column)

Sample size:100-200ml Plasmid DNA Maxiprep Kit (High

Purifity, Silica-precipitation)


Endotoxin-Free

Plasmid DNA Maxiprep Kit

(Endotoxin-Free)

Yeast Sample size:1-5ml Yeast Plasmid DNA Miniprep Kit

DN

A P

uri

fic

ati

on

Kit

s

3

40



Dongsheng Biotech offers a broad range of kits for the isolation and

purification of genomic and plasmid DNA from a wide variety of samples,

including tissue, cells , blood, serum, bacterial, yeast, and plants samples .

We provide three series for different materials purification:'

Plasmid DNA Extraction Kits

DNA Product Purification Kits

Genimic DNA Purification Kits

DN

A P

uri

fic

ati

on

Kit

s

3

39



Guides of DNA Purification Kits

Quick Genomic DNA Extraction

Kit

Standard

Agarose Gel Safe, Healthy

Gel Extraction Kit

Eco-Friendly Gel Extraction Kit

PCR Product/

DNA Fragment

SolutionSafe, Healthy

PCR and DNA Fragment

Purification Kit

Eco-Friendly PCR and DNA

Fragment Purification Kit

DNA Product

Purification

Genomic DNA

Purification

Insect/ Animal

Nematoda


10mg tissue

Blood

Sample size: 0.l-1ml

flesh blood/ frozen blood

Blood Genomic DNA

Extraction Kit


200ul whole bloodQuick Blood Genomic DNA

Extraction Kit

Tissue/

Cell

Bacteria

Yeast

Sample size: less than6 710mg tissue/ 10 -10

culture cell

Sample size: 0.5-2ml/6less than 10 -10 bacteria

Sample size: less than71-5x10 Yeast

Quick Tissue/Culture Cells

Genomic DNA Extraction Kit

Quick Bacteria Genomic

DNA Extraction Kit

Quick Yeast Genomic DNA

Extraction Kit

Application Material Experimental requirement Product Choose

Plasmid DNA

ExtractionBacteria

Sample size:1-5ml

Sample size:1-5ml Safe, Healthy



Simple, Convenient


Plasmid DNA Maxiprep Kit (High

Purifity, Silica-membrant Spin

Column)

Sample size:100-200ml Plasmid DNA Maxiprep Kit (High

Purifity, Silica-precipitation)


Endotoxin-Free

Plasmid DNA Maxiprep Kit

(Endotoxin-Free)

Yeast Sample size:1-5ml Yeast Plasmid DNA Miniprep Kit

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Plasmid Extraction Series

Genomic DNA Purification Serious

Quick Genomic DNA Extraction Kit

Blood Genomic DNA Extraction Kit

Quick Blood Genomic DNA Extraction Kit

Quick Tissue/Culture Cells Genomic DNA Extraction Kit

Quick Bacteria Genomic DNA Extraction Kit

Quick Yeast Genomic DNA Extraction Kit

Marine Animal Tissue Genomic DNA Extraction Kit

Product Name Cat # Size

N1111

N1112

N1121

N1122

N1131

N1132

N1141

N1142

N1151

N1152

N1161

N1162

N1171

N1172

50 preps

100 preps

50 preps

100 preps

50 preps

100 preps

50 preps

100 preps

50 preps

100 preps

50 preps

100 preps

50 preps

100 preps



High Purity Plasmid Miniprep kit


Plasmid DNA Maxiprep Kit (High Purity, Silica-mimbrane

Spin Column)

Plasmid DNA Maxiprep Kit (High Purity, Silica preciptation)

Plasmid DNA Maxiprep Kit (Endotoxin-free)

Yeast Plasmid DNA Miniprep Kit

N1011

N1012

N1021

N1022

N1031

N1041

N1051

N1061

N1062

50 preps

100 preps

50 preps

100 preps

10 preps

10 preps

10 preps

50 preps

100 preps

PCP Product Purification Series

Gel Extraction Kit

Eco-Feiendly Gel Extraction Kit

PCR and DNA Fragment Purification Kit


N1071

N1072

N1081

N1082

N1091

N1092

50 preps

100 preps

50 preps

100 preps

50 preps

100 preps

3

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Related Products


Proteinase K (20mg/ml)

Proteinase K

(Lyophilized powder)

RNase A (20mg/ml)

RNase A (100mg/ml)

RNase A


Dnase I

N9011

N9016

N9017

N9041

N9042

N9046

N9047

N9066

1ml

100mg

1g

1ml

1ml

100mg

1g

1g


Resuspend CellsLyse andNeutralize

Bind DNA

Wash column

Elute pure DNA

Description

High Purity Plasmid Miniprep Kit is designed

for rapid and cost-effective small-scale pre-

paration of high quality plasmid DNA from

recombinant E. Coli cultures. The kit utilizes

an exclusive silicabased membrane techno-

logy in the form of a convenient spin column.

Each spin column can recover up to 40ug of

plasmid DNA. The kit can be used for any size

plasmid and cosmids purification efficiently.

Content

Size

RNase A

Resuspension Solution

Lysis Solution


Wash Buffer PB

Wash Buffer W

Elution Buffer

(10mM Tris-HCl, pH 8.5)

Mini Spin Columns

N1011

50preps

150

15ml

15ml

20ml

30ml

30ml

5ml

50each

µl

For the initial use of the kit, dilute the Wash

Buffer W with ethanol 1:1.5 (v/v).

Figure .A 5 kb PCR fragment was cloned into pCMV vector. Lane M: 1kb ladderLane 1and 2 : Plasmid DNA from overnight bacterial cultures of recombinant clones was purified using the Plasmid Miniprep Kit (N1011). Lane 3 and 4 : analyzed by double digestion with EcoRI and HindIII in 30 min at 37℃.

M 1 2 3 4 M

Feature

Fast-procedure takes only 30 min

Efficient-can get 10-40 g high copy plas-

mid DNA in one prep

High purity-purified DNA is ready for down-

stream applications

µ

Application

Fast isolat ion of high purity plasmid DNA are

ready for di rect use in al l appl icat ions, inc lud ing:

Res triction digestion

DNA Seq uencing

In vitro transcript ion

Store RNase at -20℃.

Other solutuions can be stored for up to

1 year at RT (15-25℃）

Storage

3



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N1012

100preps

300µl

30ml

30ml

40ml

60ml

2x30ml

10ml

100each

N1013

200preps

600µl

60ml

60ml

80ml

120ml

3x40ml

20ml

200each

Plasmid Extraction Series

Genomic DNA Purification Serious

Quick Genomic DNA Extraction Kit

Blood Genomic DNA Extraction Kit


Quick Tissue/Culture Cells Genomic DNA Extraction Kit

Quick Bacteria Genomic DNA Extraction Kit

Quick Yeast Genomic DNA Extraction Kit

Marine Animal Tissue Genomic DNA Extraction Kit


N1111

N1112

N1121

N1122

N1131

N1132

N1141

N1142

N1151

N1152

N1161

N1162

N1171

N1172

50 preps

100 preps

50 preps

100 preps

50 preps

100 preps

50 preps

100 preps

50 preps

100 preps

50 preps

100 preps

50 preps

100 preps



High Purity Plasmid Miniprep kit


Plasmid DNA Maxiprep Kit (High Purity, Silica-mimbrane

Spin Column)

Plasmid DNA Maxiprep Kit (High Purity, Silica preciptation)

Plasmid DNA Maxiprep Kit (Endotoxin-free)

Yeast Plasmid DNA Miniprep Kit

N1011

N1012

N1021

N1022

N1031

N1041

N1051

N1061

N1062

50 preps

100 preps

50 preps

100 preps

10 preps

10 preps

10 preps

50 preps

100 preps

PCP Product Purification Series

Gel Extraction Kit

Eco-Feiendly Gel Extraction Kit

PCR and DNA Fragment Purification Kit


N1071

N1072

N1081

N1082

N1091

N1092

50 preps

100 preps

50 preps

100 preps

50 preps

100 preps

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Related Products



Proteinase K


RNase A (20mg/ml)

RNase A (100mg/ml)

RNase A


Dnase I

N9011

N9016

N9017

N9041

N9042

N9046

N9047

N9066

1ml

100mg

1g

1ml

1ml

100mg

1g

1g



Bind DNA

Wash column

Elute pure DNA

Description

High Purity Plasmid Miniprep Kit is designed

for rapid and cost-effective small-scale pre-

paration of high quality plasmid DNA from

recombinant E. Coli cultures. The kit utilizes

an exclusive silicabased membrane techno-

logy in the form of a convenient spin column.

Each spin column can recover up to 40ug of

plasmid DNA. The kit can be used for any size

plasmid and cosmids purification efficiently.

Content

Size

RNase A

Resuspension Solution

Lysis Solution


Wash Buffer PB

Wash Buffer W

Elution Buffer


Mini Spin Columns

N1011

50preps

150

15ml

15ml

20ml

30ml

30ml

5ml

50each

µl


Buffer W with ethanol 1:1.5 (v/v).

Figure .A 5 kb PCR fragment was cloned into pCMV vector. Lane M: 1kb ladderLane 1and 2 : Plasmid DNA from overnight bacterial cultures of recombinant clones was purified using the Plasmid Miniprep Kit (N1011). Lane 3 and 4 : analyzed by double digestion with EcoRI and HindIII in 30 min at 37℃.

M 1 2 3 4 M

Feature

Fast-procedure takes only 30 min

Efficient-can get 10-40 g high copy plas-

mid DNA in one prep

High purity-purified DNA is ready for down-

stream applications

µ

Application

Fast isolat ion of high purity plasmid DNA are

ready for di rect use in al l appl icat ions, inc lud ing:

Res triction digestion

DNA Seq uencing

In vitro transcript ion

Store RNase at -20℃.

Other solutuions can be stored for up to

1 year at RT (15-25℃）

Storage

3



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N1012

100preps

300µl

30ml

30ml

40ml

60ml

2x30ml

10ml

100each

N1013

200preps

600µl

60ml

60ml

80ml

120ml

3x40ml

20ml

200each

Gel Extraction Kit

Excise gel slice with DNA fragment

Add Binding Solution to the gel slice. Incubate 10 min at 50-60℃

Transfer the mixture to the Quick Gel Extraction Columns

CentrifugeDiscard supernatant

Resuspend in wash bufferCentrifugeDiscard wash bufferRepeat 2X

Resuspend in TE bufferIncubate 5 min at 65

Centrifuge and store the micr-ocentrifuge tube containing theeluted DNA at 4 or 20

Content

Size

Binding Buffer BD

Wash Buffer PE

Elution Buffer

(10mM Tris-HCl,pH 8.5)

Spin Columns

N1071

50preps

20ml

15ml

2.5ml

50each

N1072

100preps

40ml

2x15ml

5ml

100each


Buffer PE with ethanol 1:4 (v/v)Feature

Fast-procedure takes only 15 min.

Efficient-gather up to 85% recoveries in

the range of 50bp-40kb.

Safe-not involve phenol extraction

High Purity-purified DNA is ready for

downstream applications.

Fast and efficient extraction of high purity

DNA fragments are ready for direct use in

all applications, including:

Storage

All solutions can be stored for up to 1year

RT (15-25℃).

Restriction digestion

PCR

DNA sequencing

In vitro transcription

Application

TMFigure . Recovery of DS 5000 individual 100-5000 bpDNA fragments from a 1% agarose gel using the DNA Gel M 1 2 3 4 5 6 7 8 9 M

TMLane M: DS 5000 ; Lane1:100bp; Lane 2: 250bp;Lane 3: 500bp; Lane 4: 750bp; Lane 5: 1000bp; Lane 6: 1500bp; Lane 7: 2000bp; Lane 8: 3000bp; Lane 9: 5000bp.

.Extraction Kit.

Description

Gel Ext raction Ki t is dees igned to extract and

purify DNA fragments of 50bp to 40kb from s

tandard or low- mel t agarose ge ls in ei ther Tris

acetate (TAE) or Tris borate (TBE) . Thi s mem-

brane-based system, whi ch can bind up to

40ug DNA, al lows recovery of isolated DNA

fragments in as little as 20 minutes.

3


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N10732

200preps

80ml

3x20ml

10ml

200each



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Content

Size

Solution DS

Solution QS

Proteinase K

Wash Buffer PS

Wash Buffer PE

Elution Buffer


Spin Columns

N1131

50preps

15ml

20ml

1ml

30ml

15ml

5ml

50each

N1132

100preps

30ml

40ml

2ml

60ml

30ml

10ml

100each


Buffer PE with ethanol 1:3 (v/v).

Feature

Fast--procedure takes only 30 min

High Efficient--5 g D

Simple--no need to remove erythrocyte from

the whole blood

Safe--no phenol extraction step

µ NA from 200µl whole

blood

Fast and efficient extraction of high

purity DNA fragments are ready for

direct use in all applications, including:

Storage

Store proteinase K at -20℃. Other solutions

can be stored for up to 1year at RT (15-25℃).


PCR

labeling


Application


Bind DNA

Wash column

Elute pure DNA

bloodSample preparation

Figure . Extract chicken blood DNA and human blood DNA by using the Quick Blood Genomic DNA Extraction Kit.

Lane M: Lambda DNA/Lane1: chicken blood DNALane 2: Lane 3: human blood DNALane 4:

Hind III

EcoR I restriction product for purified chicken blood DN

EcoR I restriction product for purified human blood DNA

M 1 2 3 4 M

Description


provides a simple and rapid method for high

quality genomic DNA purification from whole

blood. The Genomic DNA system uses the

silica-gel-menbrane technology for simple

and fast isolation for genomic DNA without

phenol/choroform.

Gel Extraction Kit

Excise gel slice with DNA fragment

Add Binding Solution to the gel slice. Incubate 10 min at 50-60℃

Transfer the mixture to the Quick Gel Extraction Columns

CentrifugeDiscard supernatant

Resuspend in wash bufferCentrifugeDiscard wash bufferRepeat 2X

Resuspend in TE bufferIncubate 5 min at 65

Centrifuge and store the micr-ocentrifuge tube containing theeluted DNA at 4 or 20

Content

Size

Binding Buffer BD

Wash Buffer PE

Elution Buffer

(10mM Tris-HCl,pH 8.5)

Spin Columns

N1071

50preps

20ml

15ml

2.5ml

50each

N1072

100preps

40ml

2x15ml

5ml

100each


Buffer PE with ethanol 1:4 (v/v)Feature

Fast-procedure takes only 15 min.

Efficient-gather up to 85% recoveries in

the range of 50bp-40kb.

Safe-not involve phenol extraction

High Purity-purified DNA is ready for

downstream applications.

Fast and efficient extraction of high purity

DNA fragments are ready for direct use in

all applications, including:

Storage

All solutions can be stored for up to 1year

RT (15-25℃).


PCR

DNA sequencing


Application

TMFigure . Recovery of DS 5000 individual 100-5000 bpDNA fragments from a 1% agarose gel using the DNA Gel M 1 2 3 4 5 6 7 8 9 M

TMLane M: DS 5000 ; Lane1:100bp; Lane 2: 250bp;Lane 3: 500bp; Lane 4: 750bp; Lane 5: 1000bp; Lane 6: 1500bp; Lane 7: 2000bp; Lane 8: 3000bp; Lane 9: 5000bp.

.Extraction Kit.

Description

Gel Ext raction Ki t is dees igned to extract and

purify DNA fragments of 50bp to 40kb from s

tandard or low- mel t agarose ge ls in ei ther Tris

acetate (TAE) or Tris borate (TBE) . Thi s mem-

brane-based system, whi ch can bind up to

40ug DNA, al lows recovery of isolated DNA

fragments in as little as 20 minutes.

3


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N10732

200preps

80ml

3x20ml

10ml

200each



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Content

Size

Solution DS

Solution QS

Proteinase K

Wash Buffer PS

Wash Buffer PE

Elution Buffer


Spin Columns

N1131

50preps

15ml

20ml

1ml

30ml

15ml

5ml

50each

N1132

100preps

30ml

40ml

2ml

60ml

30ml

10ml

100each


Buffer PE with ethanol 1:3 (v/v).

Feature

Fast--procedure takes only 30 min

High Efficient--5 g D

Simple--no need to remove erythrocyte from

the whole blood

Safe--no phenol extraction step

µ NA from 200µl whole

blood

Fast and efficient extraction of high

purity DNA fragments are ready for

direct use in all applications, including:

Storage

Store proteinase K at -20℃. Other solutions

can be stored for up to 1year at RT (15-25℃).


PCR

labeling


Application


Bind DNA

Wash column

Elute pure DNA

bloodSample preparation

Figure . Extract chicken blood DNA and human blood DNA by using the Quick Blood Genomic DNA Extraction Kit.

Lane M: Lambda DNA/Lane1: chicken blood DNALane 2: Lane 3: human blood DNALane 4:

Hind III

EcoR I restriction product for purified chicken blood DN

EcoR I restriction product for purified human blood DNA

M 1 2 3 4 M

Description


provides a simple and rapid method for high

quality genomic DNA purification from whole

blood. The Genomic DNA system uses the

silica-gel-menbrane technology for simple

and fast isolation for genomic DNA without

phenol/choroform.

3

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Proteinase K

Storage

N9011:1 year at room temperature, 2 years at -20℃.

N9016/N9017: 2 years at 4℃

Description

Proteinase K is an endolyt ic prot ease that cleaves

pept ide bonds at the carbox yl ic sides of al iphat ic,

arom at ic or hydrop hobic amino ac ids. The Proteinase

K is classi fied as a serine prot ease. The smal lest

pept ide to be hydrol yze d by this enzym e is a tet rapeptide.

Code

N9011

N9016

N9017

Product


Proteinase K


Proteinase K


Size

1ml

100mg

1g

R

Storage

N9041/N9042:1 year at RT, 2 years at -20℃.

N9016/N9047: 2 years at 4℃

Description

RNase A, Dnase and prot ease-free is an endoribonuclease

that spec ifica lly degrad es sing le-st randed RNA at C and U

res idues. It cleaves the phosphodiester bond between the

5’ -ribose of a nucleotide and the phosphate grou p at tached

Code

N9041

N9042

N9046

N9047

Product

RNase A (20mg/ml)

RNase A (100mg/ml)

RNase A


RNase A


Size

1ml

1ml

100mg

1g

Nase A

to the 3\-ribose of an adjacent primidine nucleotide.

D

Storage

: 2 years at 4℃

Description

Dnase I, (RNase-fre e) is an endonuclease that nonspe-

cifically cleaves DNA to re lease di-, tri - and oligonucleotide

products with 5’-phosphory lated and 3’-hydroxylated ends.

DNase I acts on single- and double- stra nded DNA, chromatin

Code

N9066

Product

DNase I


Size

1g

Nase I

Specificity activity:≥ 200Kunitz units/mg

and RNA:DNA hybrids.

Specificity activity:≥ 100Kunitz units/mg protein

Specificity activity: 40U/mg protein



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4. RNA Purification Kits

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Proteinase K

Storage

N9011:1 year at room temperature, 2 years at -20℃.

N9016/N9017: 2 years at 4℃

Description

Proteinase K is an endolyt ic prot ease that cleaves

pept ide bonds at the carbox yl ic sides of al iphat ic,

arom at ic or hydrop hobic amino ac ids. The Proteinase

K is classi fied as a serine prot ease. The smal lest

pept ide to be hydrol yze d by this enzym e is a tet rapeptide.

Code

N9011

N9016

N9017

Product


Proteinase K


Proteinase K


Size

1ml

100mg

1g

R

Storage

N9041/N9042:1 year at RT, 2 years at -20℃.

N9016/N9047: 2 years at 4℃

Description

RNase A, Dnase and prot ease-free is an endoribonuclease

that spec ifica lly degrad es sing le-st randed RNA at C and U

res idues. It cleaves the phosphodiester bond between the

5’ -ribose of a nucleotide and the phosphate grou p at tached

Code

N9041

N9042

N9046

N9047

Product

RNase A (20mg/ml)

RNase A (100mg/ml)

RNase A


RNase A


Size

1ml

1ml

100mg

1g

Nase A

to the 3\-ribose of an adjacent primidine nucleotide.

D

Storage

: 2 years at 4℃

Description

Dnase I, (RNase-fre e) is an endonuclease that nonspe-

cifically cleaves DNA to re lease di-, tri - and oligonucleotide

products with 5’-phosphory lated and 3’-hydroxylated ends.

DNase I acts on single- and double- stra nded DNA, chromatin

Code

N9066

Product

DNase I


Size

1g

Nase I

Specificity activity:≥ 200Kunitz units/mg

and RNA:DNA hybrids.

Specificity activity:≥ 100Kunitz units/mg protein

Specificity activity: 40U/mg protein



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4. RNA Purification Kits

RNA Purification Kits

Ribonucleic acid (RNA) is a biologically important type of molecule that

consists of a long chain of nucleotide units. RNA is transcribed from DNA

by enzyme called RNA Polymerase and is generally further processed

Obtaining high quality, intact RNA is the first and often the most critical

step in performing many fundamental molecular biology experiments,

including Northern analysis, nuclease protection assays, RT-PCR, RNA

mapping, in vitro translation and cDNA library construction. To be success-

ful, however, the RNA isolation procedure should include some important

Dongsheng Biotech offers an expansive portfolio of RNA Purification

products that now includes purification and stabilization products. Our

unique and trusted TRAzol, General RNA Extraction technologies provide

high yields and quantity demanded by our RNA Research team. We offer

products for RNA extraction and stabilization, as well as for purifying total

by other enzymes. RNA is central to Protein synthesis.

RNA Summarization

steps both before and after and actual RNA purification.

RNA from animals, plants, bacterias and cells.

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TRAzol

TRAzol

R1021

20ml1

R1022

100ml

Storage

Store at 2-8℃, propect from light.

TRAzol is stable for up to 12 months when

properly stored.

The isolated RNA is suitable for any

downstream application, including:

RT-PCR

Amplification for array analysis

Hybridization assays

In vitro translation

Application

Reagents required, but not supplied.

Chloroform

Isopropyl alcohol

75% ethanol (RNA-free）

DEPC-treated water

.

.

RNA yield with different starting materials.

Starting Material

Liver tissue

Spleen tissue

Kidney tissue

Skeletal muscle or

brain tissue

Placenta

Cultured epithelial cells

Fibroblasts

Feature

Classical-used for classical RNA isolation

step

High Efficient-stable RNA yield

High Purity-purified RNA is ready for any

downstream application

Description

TRAzol Reagent is a ready-to-use reagent for

the isolation of total RNA from cells and tiss-

ues. The reagent, a mono-phasic solution of

phenol and guanidine isothiocyanate, is an

improvement the single-step RNA isolation

method. During sample homogenization or

lysis, TRAzol Reagent maintains the integrity

of the RNA, while disrupting cells and dissol-

ving cell components. TRAzol Reagent facil-

itates isolation of a variety of RNA species

of large or small molecular size.

Starting

material can be either fresh or frozen tissue, or cell samples of almost any size.


Ribonucleic acid (RNA) is a biologically important type of molecule that

consists of a long chain of nucleotide units. RNA is transcribed from DNA

by enzyme called RNA Polymerase and is generally further processed

Obtaining high quality, intact RNA is the first and often the most critical

step in performing many fundamental molecular biology experiments,

including Northern analysis, nuclease protection assays, RT-PCR, RNA

mapping, in vitro translation and cDNA library construction. To be success-

ful, however, the RNA isolation procedure should include some important

Dongsheng Biotech offers an expansive portfolio of RNA Purification

products that now includes purification and stabilization products. Our

unique and trusted TRAzol, General RNA Extraction technologies provide

high yields and quantity demanded by our RNA Research team. We offer

products for RNA extraction and stabilization, as well as for purifying total

by other enzymes. RNA is central to Protein synthesis.

RNA Summarization

steps both before and after and actual RNA purification.

RNA from animals, plants, bacterias and cells.

RN

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TRAzol

TRAzol

R1021

20ml1

R1022

100ml

Storage


TRAzol is stable for up to 12 months when

properly stored.

The isolated RNA is suitable for any

downstream application, including:

RT-PCR

Amplification for array analysis


In vitro translation

Application


Chloroform

Isopropyl alcohol

75% ethanol (RNA-free）

DEPC-treated water

.

.

RNA yield with different starting materials.

Starting Material

Liver tissue

Spleen tissue

Kidney tissue

Skeletal muscle or

brain tissue

Placenta

Cultured epithelial cells

Fibroblasts

Feature

Classical-used for classical RNA isolation

step

High Efficient-stable RNA yield

High Purity-purified RNA is ready for any

downstream application

Description

TRAzol Reagent is a ready-to-use reagent for

the isolation of total RNA from cells and tiss-

ues. The reagent, a mono-phasic solution of

phenol and guanidine isothiocyanate, is an

improvement the single-step RNA isolation

method. During sample homogenization or

lysis, TRAzol Reagent maintains the integrity

of the RNA, while disrupting cells and dissol-

ving cell components. TRAzol Reagent facil-

itates isolation of a variety of RNA species

of large or small molecular size.

Starting

material can be either fresh or frozen tissue, or cell samples of almost any size.


4

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General RNA Extraction Kit

Feature

High Efficient-Stable RNA yield.

Reliable performance of high-quality

purified total RNA in downstream appli-

cations.

Storage


Kit contents are stable for up to

12 months when properly stored.

Content

Size

Solution RL

Wash Buffer RPI

Wash Buffer RW

DEPC-treated water

Rnase-free spin culumn

RNase-free

microcentrifuge tube

R1051

50preps

60ml

18ml

12ml

10ml

50each

50each

The isolated RNA is suitable for any down-

stream application, including:

RT-PCR

Amplification for microarray analysis


In vitro transation

Application


Chloroform

Ethanol (96-100%)


Buffer RPI with ethanol 3:2 (v/v), and dilute

the Wash Buffer RW with ethanol 1:4(v/v).

1 2 3 4 Figure . Extract different species of total RNAby using the Quick Genomic DNA Extraction Kit.

Lane1: Mouse tissue RNA

Lane 2: Zebrafish tissue RNA

Lane3: Leguminosae leaf tissue RNA

Lane4: Nicotianatabacum leaf tissue RNA

Description

Gen eral RNA Extraction Ki t can isolate

total RNA from a wide range of sampl e

types and amou nts. In general, sampl es

are lysed and then homog enized in the

presence of guanidinium isothiocyanate,

a chaotropic salt capable of protecting

the RNA from endogenous RNa se. Af ter

homog enization, ethanol is added to the

sampl e. The sampl e is then processed

through a spin column containing a clean

silica-based membr ane to which the RNA

binds. Any impu rities are effectively rem-

oved by subsequent washing. The purified

total RNA is then eluted in RNa se-free

water.

5. Silica Spin Column

Code

Mini Spin Column I C1011 1000pcs 45 µg


Features

Silica membrane based spin column

Compatible with hot brand extraction buffers

Economical for large sample process

High binding capability

High purity of DNA

Wide range of DNA fragment size

Convenient for use

Introduction

Dongsheng Biotech Silica Spin Columns are packed with glass fiber membranes to

rapidly purify cucleic acid using a glass fiber-based extraction method. These columns

can be used to purify single- or double- stranded DNA/RNA from contaminarlts such as

salts, solvents, enzymes or proteins. Nucleic acids are bound to the glass fiber under

high chaotropic salt concentrations, salts and umpurities are removed during the wash

steps. Pure DNA/RNA is eluted from the membrane using water or TE buffer.

Size Binding Ability Application

Purification of PCR products

Extraction of Agarose gel

Extraction of Genomic DNA

Extraction of Plasmid DNA



Extraction of Plasmid DNAMaxi Spin Column C3011 100pcs

Mini Spin Column II C1021 1000pcs 50 µg

Mini Spin Column III C1031 1000pcs 55 µg

1000 µg

Product

5

Sil

ica

Sp

in C

olu

mn

50E-mail: [email protected] Fax: +86-20-87791381

Silica Spin Column


4

RN

A P

uri

fic

ati

on

Kit

s

General RNA Extraction Kit

Feature

High Efficient-Stable RNA yield.

Reliable performance of high-quality

purified total RNA in downstream appli-

cations.

Storage


Kit contents are stable for up to

12 months when properly stored.

Content

Size

Solution RL

Wash Buffer RPI

Wash Buffer RW

DEPC-treated water

Rnase-free spin culumn

RNase-free

microcentrifuge tube

R1051

50preps

60ml

18ml

12ml

10ml

50each

50each

The isolated RNA is suitable for any down-

stream application, including:

RT-PCR

Amplification for microarray analysis


In vitro transation

Application


Chloroform

Ethanol (96-100%)


Buffer RPI with ethanol 3:2 (v/v), and dilute

the Wash Buffer RW with ethanol 1:4(v/v).

1 2 3 4 Figure . Extract different species of total RNAby using the Quick Genomic DNA Extraction Kit.

Lane1: Mouse tissue RNA

Lane 2: Zebrafish tissue RNA

Lane3: Leguminosae leaf tissue RNA

Lane4: Nicotianatabacum leaf tissue RNA

Description

Gen eral RNA Extraction Ki t can isolate

total RNA from a wide range of sampl e

types and amou nts. In general, sampl es

are lysed and then homog enized in the

presence of guanidinium isothiocyanate,

a chaotropic salt capable of protecting

the RNA from endogenous RNa se. Af ter

homog enization, ethanol is added to the

sampl e. The sampl e is then processed

through a spin column containing a clean

silica-based membr ane to which the RNA

binds. Any impu rities are effectively rem-

oved by subsequent washing. The purified

total RNA is then eluted in RNa se-free

water.


Code

Mini Spin Column I C1011 1000pcs 45 µg


Features

Silica membrane based spin column

Compatible with hot brand extraction buffers

Economical for large sample process

High binding capability

High purity of DNA

Wide range of DNA fragment size

Convenient for use

Introduction

Dongsheng Biotech Silica Spin Columns are packed with glass fiber membranes to

rapidly purify cucleic acid using a glass fiber-based extraction method. These columns

can be used to purify single- or double- stranded DNA/RNA from contaminarlts such as

salts, solvents, enzymes or proteins. Nucleic acids are bound to the glass fiber under

high chaotropic salt concentrations, salts and umpurities are removed during the wash

steps. Pure DNA/RNA is eluted from the membrane using water or TE buffer.

Size Binding Ability Application

Purification of PCR products

Extraction of Agarose gel





Extraction of Plasmid DNAMaxi Spin Column C3011 100pcs

Mini Spin Column II C1021 1000pcs 50 µg

Mini Spin Column III C1031 1000pcs 55 µg

1000 µg

Product

5

Sil

ica

Sp

in C

olu

mn

50E-mail: [email protected] Fax: +86-20-87791381

Silica Spin Column

Documents

Contents Introduction - Bohemia Genetics